MD07G1009200.v1.1

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001633
Physical Location & Seq
Forward (+)
861424 .. 864676
3253 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1009200.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGATATGGGCAACAATGGTTGATCAGGTTCAACATCCTTCTGTCGTCCAGAAGGTAGCTGGCCAGCTCCTTCAGCAATCTATCCAGGGTTACGATAATGGCTTCCAAAGACCAGGAATGTACCAAAGACGTGCTTATGGGAACTACACAAATGCTGCACTGCAGTATCCCTTCATGCCAGCATGCAGAGCTACCACTGATATGTCTTTGGTTCCATCAACTGCATCTCCTATCTTTGTTCAAGCCCCTGCGGAGAAAGGCCACTTTCTTATTGATTTTCTCATGGGCGGAGTCTCTGCTGCTGTATCTAAAACTGCTGCTGCCCCAATTGAGCGTGTCAAGCTGTTGATTCAGAACCAGGATGAGATGATCAAAACTGGTCGTTTGTCTCAACCCTACAAGGGCATTGGAGAGTGCTTTAGCCGTACAATCAAGGAAGAGGGTTTTGGTTCATTGTGGAGAGGGAACACTGCCAATGTTATCCGTTACTTCCCCACTCAGGCCTTGAACTTTGCATTCAAGGATTACTTCAAGAGGCTGTTCAACTTCAAGAAAGACAGGGACGGTTATTGGAAATGGTTTGCTGGTAACTTGGCTTCAGGAGGAGAAAGACAATTCAATGGTCTGGTTGATGTCTACAGGAAGACATTGAAATCTGATGGAATTGCCGGTCTTTACCGTGGATTCAACATCTCTTGTGTTGGAATTATTGTCTACCGTGGTCTCTATTTTGGACTGTACGACTCACTGAAGCCTGTTGTCCTGACTGGAGGCTTGCAGTTGCTAGCTCAATTTTGGTTTTTTATTACGTTCTGTGATAGTTTCTTCGCTAGCTTTGCCCTCGGTTGGCTCATCACCAATGGTGCTGGTCTTGCATCCTACCCAATTGACACTGTCCGCAGAAGAATGATGATGACCTCCGGTGAAGCCGTCAAGTACAAGAGTTCCCTGGATGCCTTCCAACAAATCTTGAAGAATGAGGGTGCCAAGTCTCTCTTCAAGGGTGCTGGTGCTAACATCCTCCGTGCCATTGCTGGTGCTGGTGTGCTGTCTGGTTATGACAAGCTACAGCTGATTGTGTTCGGCAAGAAGTACGGATCTGGTTCCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000295 GO:0003674 GO:0005215 GO:0005346 GO:0005347 GO:0005471 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006839 GO:0006855 GO:0006862 GO:0006996 GO:0007005 GO:0007006 GO:0008150 GO:0008509 GO:0008514 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0015075 GO:0015215 GO:0015216 GO:0015217 GO:0015238 GO:0015291 GO:0015297 GO:0015301 GO:0015605 GO:0015711 GO:0015748 GO:0015865 GO:0015866 GO:0015867 GO:0015868 GO:0015893 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0016043 GO:0019866 GO:0022804 GO:0022857 GO:0030312 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034220 GO:0042221 GO:0042493 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046902 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051503 GO:0055085 GO:0061024 GO:0065007 GO:0065008 GO:0070013 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0090559 GO:0098588 GO:0098656 GO:0098805 GO:0099516 GO:1901264 GO:1901505 GO:1901679
KEGG Pathways
Metabolic & Signaling
ko00514 ko04020 ko04022 ko04217 ko04218 ko05012 ko05016 ko05166 map00514 map04020 map04022 map04217 map04218 map05012 map05016 map05166
Pfam Domains
Protein Families
Mito_carr

Protein Analysis

371

Amino Acids

40.96

Weight (kDa)

9.69

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 88 - 184 3.6e-26 Mitochondrial carrier protein
Mito_carr PF00153 203 - 254 7.9e-11 Mitochondrial carrier protein
Mito_carr PF00153 274 - 361 4.9e-17 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 983
AccI GTMKAC 2 cut(s) 636, 714
AciI CCGC 3 cut(s) 251, 288, 898
AclWI GGATC 1 cut(s) 1105
AcoI YGGCCR 1 cut(s) 61
AcuI CTGAAG 3 cut(s) 56, 582, 770
AfaI GTAC 5 cut(s) 122, 427, 740, 938, 1094
AfiI CCNNNNNNNGG 3 cut(s) 401, 499, 846
AjiI CACGTC 1 cut(s) 131
AjnI CCWGG 4 cut(s) 84, 112, 357, 948
AjuI GAANNNNNNNTTGG 4 cut(s) 429, 461, 980, 1012
AluBI AGCT 8 cut(s) 59, 67, 191, 343, 788, 834, 1066, 1072
AluI AGCT 8 cut(s) 59, 67, 191, 343, 788, 834, 1066, 1072
Alw26I GTCTC 4 cut(s) 298, 393, 728, 996
AlwI GGATC 1 cut(s) 1105
AoxI GGCC 3 cut(s) 61, 259, 501
ApeKI GCWGC 4 cut(s) 155, 299, 317, 320
AsuHPI GGTGA 2 cut(s) 847, 935
AsuNHI GCTAGC 2 cut(s) 784, 830
BaeI ACNNNNGTAYC 2 cut(s) 149, 182
BalI TGGCCA 1 cut(s) 63
BanI GGYRCC 1 cut(s) 983
BarI GAAGNNNNNNTAC 2 cut(s) 580, 612
BbsI GAAGAC 1 cut(s) 650
BbvI GCAGC 4 cut(s) 142, 286, 304, 307
BccI CCATC 2 cut(s) 223, 653
BceAI ACGGC 2 cut(s) 408, 914
BciT130I CCWGG 4 cut(s) 86, 114, 359, 950
BciVI GTATCC 1 cut(s) 177
BclI TGATCA 2 cut(s) 22, 369
BcoDI GTCTC 4 cut(s) 298, 393, 728, 996
BfaI CTAG 2 cut(s) 785, 831
BfmI CTRYAG 3 cut(s) 161, 637, 1067
BfuI GTATCC 1 cut(s) 177
BisI GCNGC 4 cut(s) 156, 300, 318, 321
BlsI GCNGC 4 cut(s) 157, 301, 319, 322
Bme1390I CCNGG 4 cut(s) 86, 114, 359, 950
BmgBI CACGTC 1 cut(s) 131
BmiI GGNNCC 3 cut(s) 213, 985, 1105
BmrFI CCNGG 4 cut(s) 86, 114, 359, 950
BmsI GCATC 3 cut(s) 233, 884, 943
BmtI GCTAGC 2 cut(s) 788, 834
BpiI GAAGAC 1 cut(s) 650
BpmI CTGGAG 1 cut(s) 789
BsaI GGTCTC 1 cut(s) 728
BsaJI CCNNGG 5 cut(s) 85, 679, 718, 841, 948
BsaWI WCCGGW 2 cut(s) 920, 1106
BsaXI ACNNNNNCTCC 2 cut(s) 211, 241
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 499, 846
Bse118I RCCGGY 1 cut(s) 668
Bse1I ACTGG 2 cut(s) 382, 772
Bse3DI GCAATG 1 cut(s) 1029
BseBI CCWGG 4 cut(s) 86, 114, 359, 950
BseDI CCNNGG 5 cut(s) 85, 679, 718, 841, 948
BseGI GGATG 5 cut(s) 34, 367, 875, 958, 1017
BseLI CCNNNNNNNGG 3 cut(s) 401, 499, 846
BseMI GCAATG 1 cut(s) 1029
BseMII CTCAG 1 cut(s) 512
BseNI ACTGG 2 cut(s) 382, 772
BseRI GAGGAG 1 cut(s) 618
BseXI GCAGC 4 cut(s) 142, 286, 304, 307
BsgI GTGCAG 1 cut(s) 141
BshFI GGCC 3 cut(s) 63, 261, 503
BshNI GGYRCC 1 cut(s) 983
BsiSI CCGG 3 cut(s) 669, 921, 1107
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 3 cut(s) 401, 499, 846
BsmAI GTCTC 4 cut(s) 298, 393, 728, 996
BsmFI GGGAC 1 cut(s) 575
BsmI GAATGC 1 cut(s) 515
BsnI GGCC 3 cut(s) 63, 261, 503
Bso31I GGTCTC 1 cut(s) 728
Bsp143I GATC 3 cut(s) 22, 369, 1097
BspACI CCGC 3 cut(s) 251, 288, 898
BspANI GGCC 3 cut(s) 63, 261, 503
BspCNI CTCAG 1 cut(s) 511
BspLI GGNNCC 3 cut(s) 213, 985, 1105
BspMAI CTGCAG 1 cut(s) 165
BspOI GCTAGC 2 cut(s) 788, 834
BspPI GGATC 1 cut(s) 1105
BspT107I GGYRCC 1 cut(s) 983
BspTNI GGTCTC 1 cut(s) 728
BsrDI GCAATG 1 cut(s) 1029
BsrFI RCCGGY 1 cut(s) 668
BsrI ACTGG 2 cut(s) 382, 772
BssAI RCCGGY 1 cut(s) 668
BssECI CCNNGG 5 cut(s) 85, 679, 718, 841, 948
BssMI GATC 3 cut(s) 22, 369, 1097
Bst2UI CCWGG 4 cut(s) 86, 114, 359, 950
Bst4CI ACNGT 5 cut(s) 566, 680, 719, 738, 895
Bst6I CTCTTC 2 cut(s) 432, 1001
BstC8I GCNNGC 7 cut(s) 61, 65, 180, 184, 776, 786, 832
BstDEI CTNAG 1 cut(s) 498
BstDSI CCRYGG 2 cut(s) 679, 718
BstF5I GGATG 5 cut(s) 34, 367, 875, 958, 1017
BstKTI GATC 3 cut(s) 25, 372, 1100
BstMAI GTCTC 4 cut(s) 298, 393, 728, 996
BstMBI GATC 3 cut(s) 22, 369, 1097
BstMWI GCNNNNNNNGC 5 cut(s) 73, 188, 340, 836, 872
BstNI CCWGG 4 cut(s) 86, 114, 359, 950
BstNSI RCATGY 1 cut(s) 186
BstSCI CCNGG 4 cut(s) 84, 112, 357, 948
BstSFI CTRYAG 3 cut(s) 161, 637, 1067
BstV1I GCAGC 4 cut(s) 142, 286, 304, 307
BstV2I GAAGAC 1 cut(s) 650
BstX2I RGATCY 1 cut(s) 1097
BstYI RGATCY 1 cut(s) 1097
BsuI GTATCC 1 cut(s) 177
BsuRI GGCC 3 cut(s) 63, 261, 503
BtgI CCRYGG 2 cut(s) 679, 718
BtrI CACGTC 1 cut(s) 131
BtsCI GGATG 5 cut(s) 34, 367, 875, 958, 1017
BtsI GCAGTG 2 cut(s) 158, 468
BtsIMutI CAGTG 5 cut(s) 158, 195, 468, 746, 891
Cac8I GCNNGC 7 cut(s) 61, 65, 180, 184, 776, 786, 832
Cfr10I RCCGGY 1 cut(s) 668
Csp6I GTAC 5 cut(s) 121, 426, 739, 937, 1093
CviAII CATG 3 cut(s) 175, 183, 283
CviQI GTAC 5 cut(s) 121, 426, 739, 937, 1093
DdeI CTNAG 1 cut(s) 498
DpnI GATC 3 cut(s) 24, 371, 1099
DpnII GATC 3 cut(s) 22, 369, 1097
EaeI YGGCCR 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 432, 1001
EarI CTCTTC 2 cut(s) 432, 1001
EciI GGCGGA 1 cut(s) 303
Eco147I AGGCCT 1 cut(s) 503
Eco31I GGTCTC 1 cut(s) 728
Eco57I CTGAAG 3 cut(s) 56, 582, 770
EcoRII CCWGG 4 cut(s) 84, 112, 357, 948
FaeI CATG 3 cut(s) 178, 186, 286
FaiI YATR 7 cut(s) 7, 138, 176, 184, 203, 284, 1059
FalI AAGNNNNNCTT 6 cut(s) 118, 150, 512, 544, 980, 1012
FaqI GGGAC 1 cut(s) 575
FatI CATG 3 cut(s) 174, 182, 282
FbaI TGATCA 2 cut(s) 22, 369
FblI GTMKAC 2 cut(s) 636, 714
Fnu4HI GCNGC 4 cut(s) 156, 300, 318, 321
FokI GGATG 5 cut(s) 21, 374, 862, 965, 1004
Fsp4HI GCNGC 4 cut(s) 156, 300, 318, 321
FspBI CTAG 2 cut(s) 785, 831
GluI GCNGC 4 cut(s) 156, 300, 318, 321
GsuI CTGGAG 1 cut(s) 789
HaeIII GGCC 3 cut(s) 63, 261, 503
HapII CCGG 3 cut(s) 669, 921, 1107
Hin1II CATG 3 cut(s) 178, 186, 286
HinfI GANTC 4 cut(s) 291, 349, 684, 743
HpaII CCGG 3 cut(s) 669, 921, 1107
HphI GGTGA 2 cut(s) 847, 935
Hpy166II GTNNAC 2 cut(s) 637, 715
Hpy188I TCNGA 2 cut(s) 354, 658
Hpy188III TCNNGA 6 cut(s) 49, 532, 550, 600, 763, 970
Hpy8I GTNNAC 2 cut(s) 637, 715
HpyAV CCTTC 5 cut(s) 46, 48, 80, 181, 967
HpyCH4III ACNGT 5 cut(s) 566, 680, 719, 738, 895
HpyCH4IV ACGT 2 cut(s) 130, 809
HpyCH4V TGCA 7 cut(s) 158, 163, 186, 224, 515, 778, 875
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 188, 340, 836, 872
HpyF3I CTNAG 1 cut(s) 498
HpySE526I ACGT 2 cut(s) 130, 809
Hsp92II CATG 3 cut(s) 178, 186, 286
Ksp22I TGATCA 2 cut(s) 22, 369
Kzo9I GATC 3 cut(s) 22, 369, 1097
LmnI GCTCC 1 cut(s) 72
Lsp1109I GCAGC 4 cut(s) 142, 286, 304, 307
LweI GCATC 3 cut(s) 233, 884, 943
MaeI CTAG 2 cut(s) 785, 831
MaeII ACGT 2 cut(s) 130, 809
MaeIII GTNAC 3 cut(s) 89, 485, 587
MalI GATC 3 cut(s) 24, 371, 1099
MboI GATC 3 cut(s) 22, 369, 1097
MboII GAAGA 6 cut(s) 449, 655, 817, 915, 985, 988
MfeI CAATTG 2 cut(s) 327, 885
MflI RGATCY 1 cut(s) 1097
MlsI TGGCCA 1 cut(s) 63
MluCI AATT 6 cut(s) 327, 614, 663, 705, 791, 885
MluNI TGGCCA 1 cut(s) 63
MlyI GAGTC 2 cut(s) 300, 737
MmeI TCCRAC 2 cut(s) 682, 985
MnlI CCTC 9 cut(s) 433, 455, 528, 596, 764, 851, 928, 973, 1031
Mox20I TGGCCA 1 cut(s) 63
MscI TGGCCA 1 cut(s) 63
MseI TTAA 1 cut(s) 1111
MslI CAYNNNNRTG 1 cut(s) 200
Msp20I TGGCCA 1 cut(s) 63
MspA1I CMGCKG 1 cut(s) 1072
MspI CCGG 3 cut(s) 669, 921, 1107
MspR9I CCNGG 4 cut(s) 86, 114, 359, 950
MunI CAATTG 2 cut(s) 327, 885
Mva1269I GAATGC 1 cut(s) 515
MvaI CCWGG 4 cut(s) 86, 114, 359, 950
MwoI GCNNNNNNNGC 5 cut(s) 73, 188, 340, 836, 872
NdeII GATC 3 cut(s) 22, 369, 1097
NheI GCTAGC 2 cut(s) 784, 830
NlaIII CATG 3 cut(s) 178, 186, 286
NlaIV GGNNCC 3 cut(s) 213, 985, 1105
NspI RCATGY 1 cut(s) 186
PaeI GCATGC 1 cut(s) 186
PceI AGGCCT 1 cut(s) 503
PctI GAATGC 1 cut(s) 515
PfeI GAWTC 2 cut(s) 349, 684
PflFI GACNNNGTC 1 cut(s) 893
PkrI GCNGC 4 cut(s) 157, 301, 319, 322
PleI GAGTC 2 cut(s) 299, 737
PpsI GAGTC 2 cut(s) 299, 737
Psp6I CCWGG 4 cut(s) 84, 112, 357, 948
PspGI CCWGG 4 cut(s) 84, 112, 357, 948
PspN4I GGNNCC 3 cut(s) 213, 985, 1105
PstI CTGCAG 1 cut(s) 165
PsuI RGATCY 1 cut(s) 1097
PsyI GACNNNGTC 1 cut(s) 893
PvuII CAGCTG 1 cut(s) 1072
RsaI GTAC 5 cut(s) 122, 427, 740, 938, 1094
RsaNI GTAC 5 cut(s) 121, 426, 739, 937, 1093
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 1 cut(s) 1111
SatI GCNGC 4 cut(s) 156, 300, 318, 321
Sau3AI GATC 3 cut(s) 22, 369, 1097
SchI GAGTC 2 cut(s) 300, 737
ScrFI CCNGG 4 cut(s) 86, 114, 359, 950
SfaNI GCATC 3 cut(s) 233, 884, 943
SfcI CTRYAG 3 cut(s) 161, 637, 1067
SmiMI CAYNNNNRTG 1 cut(s) 200
SphI GCATGC 1 cut(s) 186
Sse9I AATT 6 cut(s) 327, 614, 663, 705, 791, 885
SseBI AGGCCT 1 cut(s) 503
SsiI CCGC 3 cut(s) 251, 288, 898
SspMI CTAG 2 cut(s) 785, 831
StuI AGGCCT 1 cut(s) 503
StyD4I CCNGG 4 cut(s) 84, 112, 357, 948
TaaI ACNGT 5 cut(s) 566, 680, 719, 738, 895
TaiI ACGT 2 cut(s) 133, 812
TasI AATT 6 cut(s) 327, 614, 663, 705, 791, 885
TatI WGTACW 1 cut(s) 936
TfiI GAWTC 2 cut(s) 349, 684
Tru1I TTAA 1 cut(s) 1111
Tru9I TTAA 1 cut(s) 1111
TscAI CASTG 5 cut(s) 165, 202, 475, 753, 898
TseI GCWGC 4 cut(s) 155, 299, 317, 320
TspDTI ATGAA 2 cut(s) 163, 441
TspGWI ACGGA 3 cut(s) 473, 1013, 1110
TspRI CASTG 5 cut(s) 165, 202, 475, 753, 898
Tth111I GACNNNGTC 1 cut(s) 893
XceI RCATGY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 56
XmiI GTMKAC 2 cut(s) 636, 714
XspI CTAG 2 cut(s) 785, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.