MD07G1009000.v1.1

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001633
Physical Location & Seq
Forward (+)
844787 .. 848383
3597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1009000.v1.1.491

Sequence Viewer

Length: 1170 bp
ATGGGGCAAGTGACAATGGTTGATCAGGTTCAACATCCTTCTGTCGTCCAGAAGGTAGCTGGCCAGCTCCTTCAGCAATCTATCCAGGGTTACGATAATGGCTTCCAAAGACCAGGAATGTACCAAAGACGTGCTTATGGGAACTACACTAATGCTGCACTGCAGTATCCCTTCATGCCAGCATGCAGAGCTACCACTGATATGTCTTTGGTTCCATCAACTGCATCACCCATCTTTGTTCAAGCCCCTGCAGAGAAAGGCCACTTTCTGATTGATTTTCTCATGGGTGGAGTCTCTGCTGCTGTGTCTAAAACTGCTGCTGCCCCAATTGAGCGTGTCAAGCTGTTGATTCAAAACCAGGATGAGATGATCAAAACTGGTCGTTTGTCTCAACCCTACAAGGGCATTGGGGAGTGCTTTAGCCGTACAATCAAGGAAGAGGGTTTTGGTTCATTGTGGAGAGGGAACACTGCCAATGTTATCCGTTACTTCCCCACTCAGGCCTTGAACTTTGCATTCAAGGATTACTTCAAGAGGCTGTTCAACTTCAAGAAAGACCGGGACGGTTATTGGAAATGGTTTGCTGGTAACTTGGCTTCAGGAGGTGCTGCTGGTGCTTCTTCTCTTTTCTTTGTCTACTCGTTGGATTATGCTCGTACTCGCTTGGCTAATGATTCTAAAGCTGCAAAGAAGGGAGGAGAAAGACAATTCAATGGTCTGGTTGATGTCTACAGGAAGACTTTGAAATCTGATGGAATTGCTGGTCTTTACCGTGGATTCAACATCTCTTGTGTTGGAATTATTGTCTACCGTGGTCTCTACTTTGGACTGTATGATTCACTGAAGCCTGTTGTCCTGACTGGAGGCTTGCAGGATAGTTTCTTTGCTAGCTTTGCCCTTGGTTGGGTCATCACCAATGGTGCTGGTCTTGCATCCTACCCAATTGACACTGTCCGCAGAAGAATGATGATGACCTCCGGTGAAGCCGTCAAGTACAAGAGTTCCCTGGATGCCTTCCAACAAATCTTGAAGAACGAGGGTGCCAAGTCTCTCTTCAAGGGTGCTGGTGCTAACATCCTCCGTGCCATTGCTGGTGCTGGTGTGCTGTCTGGTTATGACAAGCTACAGCTGCTTGTGTTCGGCAAGAAGTACGGATCTGGTTCCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000295 GO:0003674 GO:0005215 GO:0005346 GO:0005347 GO:0005471 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006839 GO:0006855 GO:0006862 GO:0006996 GO:0007005 GO:0007006 GO:0008150 GO:0008509 GO:0008514 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0015075 GO:0015215 GO:0015216 GO:0015217 GO:0015238 GO:0015291 GO:0015297 GO:0015301 GO:0015605 GO:0015711 GO:0015748 GO:0015865 GO:0015866 GO:0015867 GO:0015868 GO:0015893 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0016043 GO:0019866 GO:0022804 GO:0022857 GO:0030312 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034220 GO:0042221 GO:0042493 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046902 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051503 GO:0055085 GO:0061024 GO:0065007 GO:0065008 GO:0070013 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0090559 GO:0098588 GO:0098656 GO:0098805 GO:0099516 GO:1901264 GO:1901505 GO:1901679
KEGG Pathways
Metabolic & Signaling
ko00514 ko04020 ko04022 ko04217 ko04218 ko05012 ko05016 ko05166 map00514 map04020 map04022 map04217 map04218 map05012 map05016 map05166
Pfam Domains
Protein Families
Mito_carr

Protein Analysis

390

Amino Acids

42.56

Weight (kDa)

9.78

Isoelectric Point (pI)

29.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 88 - 184 3.9e-26 Mitochondrial carrier protein
Mito_carr PF00153 192 - 285 6.6e-21 Mitochondrial carrier protein
Mito_carr PF00153 292 - 381 2e-17 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1040
AccI GTMKAC 3 cut(s) 636, 729, 807
AciI CCGC 1 cut(s) 955
AclWI GGATC 1 cut(s) 1162
AcoI YGGCCR 1 cut(s) 61
AcuI CTGAAG 3 cut(s) 56, 582, 863
AfaI GTAC 5 cut(s) 122, 427, 658, 995, 1151
AfiI CCNNNNNNNGG 4 cut(s) 401, 499, 903, 904
AjiI CACGTC 1 cut(s) 131
AjnI CCWGG 4 cut(s) 84, 112, 357, 1005
AjuI GAANNNNNNNTTGG 4 cut(s) 429, 461, 1037, 1069
AluBI AGCT 8 cut(s) 59, 67, 191, 343, 683, 891, 1123, 1129
AluI AGCT 8 cut(s) 59, 67, 191, 343, 683, 891, 1123, 1129
Alw26I GTCTC 4 cut(s) 298, 393, 821, 1053
AlwI GGATC 1 cut(s) 1162
AoxI GGCC 3 cut(s) 61, 259, 501
ApeKI GCWGC 7 cut(s) 155, 299, 317, 320, 608, 683, 1129
AsuC2I CCSGG 1 cut(s) 560
AsuHPI GGTGA 3 cut(s) 219, 904, 992
AsuNHI GCTAGC 1 cut(s) 887
BaeI ACNNNNGTAYC 2 cut(s) 149, 182
BalI TGGCCA 1 cut(s) 63
BanI GGYRCC 1 cut(s) 1040
BarI GAAGNNNNNNTAC 2 cut(s) 580, 612
BbsI GAAGAC 1 cut(s) 743
BbvI GCAGC 7 cut(s) 142, 286, 304, 307, 595, 670, 1116
BccI CCATC 3 cut(s) 223, 239, 746
BceAI ACGGC 2 cut(s) 408, 971
BciT130I CCWGG 4 cut(s) 86, 114, 359, 1007
BciVI GTATCC 1 cut(s) 177
BclI TGATCA 2 cut(s) 22, 369
BcnI CCSGG 1 cut(s) 560
BcoDI GTCTC 4 cut(s) 298, 393, 821, 1053
BfaI CTAG 1 cut(s) 888
BfmI CTRYAG 4 cut(s) 161, 249, 730, 1124
BfuI GTATCC 1 cut(s) 177
BisI GCNGC 7 cut(s) 156, 300, 318, 321, 609, 684, 1130
BlsI GCNGC 7 cut(s) 157, 301, 319, 322, 610, 685, 1131
Bme1390I CCNGG 5 cut(s) 86, 114, 359, 560, 1007
BmgBI CACGTC 1 cut(s) 131
BmiI GGNNCC 3 cut(s) 213, 1042, 1162
BmrFI CCNGG 5 cut(s) 86, 114, 359, 560, 1007
BmsI GCATC 3 cut(s) 233, 941, 1000
BmtI GCTAGC 1 cut(s) 891
BpiI GAAGAC 1 cut(s) 743
BpmI CTGGAG 1 cut(s) 882
BpuMI CCSGG 1 cut(s) 560
BsaI GGTCTC 1 cut(s) 821
BsaJI CCNNGG 5 cut(s) 85, 772, 811, 898, 1005
BsaWI WCCGGW 2 cut(s) 977, 1163
Bsc4I CCNNNNNNNGG 4 cut(s) 401, 499, 903, 904
Bse1I ACTGG 2 cut(s) 382, 865
Bse3DI GCAATG 1 cut(s) 1086
BseBI CCWGG 4 cut(s) 86, 114, 359, 1007
BseDI CCNNGG 5 cut(s) 85, 772, 811, 898, 1005
BseGI GGATG 5 cut(s) 34, 367, 932, 1015, 1074
BseLI CCNNNNNNNGG 4 cut(s) 401, 499, 903, 904
BseMI GCAATG 1 cut(s) 1086
BseMII CTCAG 1 cut(s) 512
BseNI ACTGG 2 cut(s) 382, 865
BseRI GAGGAG 1 cut(s) 711
BseXI GCAGC 7 cut(s) 142, 286, 304, 307, 595, 670, 1116
BsgI GTGCAG 1 cut(s) 141
BshFI GGCC 3 cut(s) 63, 261, 503
BshNI GGYRCC 1 cut(s) 1040
BsiSI CCGG 3 cut(s) 559, 978, 1164
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 4 cut(s) 401, 499, 903, 904
BsmAI GTCTC 4 cut(s) 298, 393, 821, 1053
BsmFI GGGAC 1 cut(s) 575
BsmI GAATGC 1 cut(s) 515
BsnI GGCC 3 cut(s) 63, 261, 503
Bso31I GGTCTC 1 cut(s) 821
Bsp143I GATC 3 cut(s) 22, 369, 1154
BspACI CCGC 1 cut(s) 955
BspANI GGCC 3 cut(s) 63, 261, 503
BspCNI CTCAG 1 cut(s) 511
BspLI GGNNCC 3 cut(s) 213, 1042, 1162
BspMAI CTGCAG 2 cut(s) 165, 253
BspOI GCTAGC 1 cut(s) 891
BspPI GGATC 1 cut(s) 1162
BspT107I GGYRCC 1 cut(s) 1040
BspTNI GGTCTC 1 cut(s) 821
BsrDI GCAATG 1 cut(s) 1086
BsrI ACTGG 2 cut(s) 382, 865
BssECI CCNNGG 5 cut(s) 85, 772, 811, 898, 1005
BssMI GATC 3 cut(s) 22, 369, 1154
BssT1I CCWWGG 1 cut(s) 898
Bst2UI CCWGG 4 cut(s) 86, 114, 359, 1007
Bst4CI ACNGT 5 cut(s) 566, 773, 812, 831, 952
Bst6I CTCTTC 2 cut(s) 432, 1058
BstC8I GCNNGC 6 cut(s) 61, 65, 180, 184, 869, 889
BstDEI CTNAG 1 cut(s) 498
BstDSI CCRYGG 2 cut(s) 772, 811
BstF5I GGATG 5 cut(s) 34, 367, 932, 1015, 1074
BstKTI GATC 3 cut(s) 25, 372, 1157
BstMAI GTCTC 4 cut(s) 298, 393, 821, 1053
BstMBI GATC 3 cut(s) 22, 369, 1154
BstMWI GCNNNNNNNGC 7 cut(s) 73, 188, 340, 614, 893, 929, 1129
BstNI CCWGG 4 cut(s) 86, 114, 359, 1007
BstNSI RCATGY 1 cut(s) 186
BstSCI CCNGG 5 cut(s) 84, 112, 357, 558, 1005
BstSFI CTRYAG 4 cut(s) 161, 249, 730, 1124
BstV1I GCAGC 7 cut(s) 142, 286, 304, 307, 595, 670, 1116
BstV2I GAAGAC 1 cut(s) 743
BstX2I RGATCY 1 cut(s) 1154
BstYI RGATCY 1 cut(s) 1154
BsuI GTATCC 1 cut(s) 177
BsuRI GGCC 3 cut(s) 63, 261, 503
BtgI CCRYGG 2 cut(s) 772, 811
BtrI CACGTC 1 cut(s) 131
BtsCI GGATG 5 cut(s) 34, 367, 932, 1015, 1074
BtsI GCAGTG 2 cut(s) 158, 468
BtsIMutI CAGTG 5 cut(s) 158, 195, 468, 839, 948
Cac8I GCNNGC 6 cut(s) 61, 65, 180, 184, 869, 889
Csp6I GTAC 5 cut(s) 121, 426, 657, 994, 1150
CviAII CATG 3 cut(s) 175, 183, 283
CviQI GTAC 5 cut(s) 121, 426, 657, 994, 1150
DdeI CTNAG 1 cut(s) 498
DpnI GATC 3 cut(s) 24, 371, 1156
DpnII GATC 3 cut(s) 22, 369, 1154
EaeI YGGCCR 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 432, 1058
EarI CTCTTC 2 cut(s) 432, 1058
Eco130I CCWWGG 1 cut(s) 898
Eco147I AGGCCT 1 cut(s) 503
Eco31I GGTCTC 1 cut(s) 821
Eco57I CTGAAG 3 cut(s) 56, 582, 863
EcoRII CCWGG 4 cut(s) 84, 112, 357, 1005
EcoT14I CCWWGG 1 cut(s) 898
ErhI CCWWGG 1 cut(s) 898
FaeI CATG 3 cut(s) 178, 186, 286
FaiI YATR 8 cut(s) 138, 176, 184, 203, 284, 651, 834, 1116
FalI AAGNNNNNCTT 6 cut(s) 118, 150, 512, 544, 1037, 1069
FaqI GGGAC 1 cut(s) 575
FatI CATG 3 cut(s) 174, 182, 282
FbaI TGATCA 2 cut(s) 22, 369
FblI GTMKAC 3 cut(s) 636, 729, 807
Fnu4HI GCNGC 7 cut(s) 156, 300, 318, 321, 609, 684, 1130
FokI GGATG 5 cut(s) 21, 374, 919, 1022, 1061
Fsp4HI GCNGC 7 cut(s) 156, 300, 318, 321, 609, 684, 1130
FspBI CTAG 1 cut(s) 888
GluI GCNGC 7 cut(s) 156, 300, 318, 321, 609, 684, 1130
GsuI CTGGAG 1 cut(s) 882
HaeIII GGCC 3 cut(s) 63, 261, 503
HapII CCGG 3 cut(s) 559, 978, 1164
Hin1II CATG 3 cut(s) 178, 186, 286
HinfI GANTC 5 cut(s) 291, 349, 674, 777, 836
HpaII CCGG 3 cut(s) 559, 978, 1164
HphI GGTGA 3 cut(s) 219, 904, 992
Hpy166II GTNNAC 3 cut(s) 637, 730, 808
Hpy188I TCNGA 2 cut(s) 270, 751
Hpy188III TCNNGA 6 cut(s) 49, 532, 550, 600, 856, 1027
Hpy8I GTNNAC 3 cut(s) 637, 730, 808
HpyAV CCTTC 6 cut(s) 46, 48, 80, 181, 685, 1024
HpyCH4III ACNGT 5 cut(s) 566, 773, 812, 831, 952
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 9 cut(s) 158, 163, 186, 224, 251, 515, 686, 871, 932
HpyF10VI GCNNNNNNNGC 7 cut(s) 73, 188, 340, 614, 893, 929, 1129
HpyF3I CTNAG 1 cut(s) 498
HpySE526I ACGT 1 cut(s) 130
Hsp92II CATG 3 cut(s) 178, 186, 286
Ksp22I TGATCA 2 cut(s) 22, 369
Kzo9I GATC 3 cut(s) 22, 369, 1154
LmnI GCTCC 1 cut(s) 72
Lsp1109I GCAGC 7 cut(s) 142, 286, 304, 307, 595, 670, 1116
LweI GCATC 3 cut(s) 233, 941, 1000
MaeI CTAG 1 cut(s) 888
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 4 cut(s) 10, 89, 485, 587
MalI GATC 3 cut(s) 24, 371, 1156
MboI GATC 3 cut(s) 22, 369, 1154
MboII GAAGA 6 cut(s) 449, 612, 748, 972, 1042, 1045
MfeI CAATTG 2 cut(s) 327, 942
MflI RGATCY 1 cut(s) 1154
MlsI TGGCCA 1 cut(s) 63
MluCI AATT 5 cut(s) 327, 707, 756, 798, 942
MluNI TGGCCA 1 cut(s) 63
MlyI GAGTC 1 cut(s) 300
MmeI TCCRAC 3 cut(s) 624, 775, 1042
MnlI CCTC 9 cut(s) 433, 455, 528, 596, 689, 857, 985, 1030, 1088
Mox20I TGGCCA 1 cut(s) 63
MscI TGGCCA 1 cut(s) 63
MseI TTAA 1 cut(s) 1168
MslI CAYNNNNRTG 1 cut(s) 200
Msp20I TGGCCA 1 cut(s) 63
MspA1I CMGCKG 1 cut(s) 1129
MspI CCGG 3 cut(s) 559, 978, 1164
MspR9I CCNGG 5 cut(s) 86, 114, 359, 560, 1007
MunI CAATTG 2 cut(s) 327, 942
Mva1269I GAATGC 1 cut(s) 515
MvaI CCWGG 4 cut(s) 86, 114, 359, 1007
MwoI GCNNNNNNNGC 7 cut(s) 73, 188, 340, 614, 893, 929, 1129
NciI CCSGG 1 cut(s) 560
NdeII GATC 3 cut(s) 22, 369, 1154
NheI GCTAGC 1 cut(s) 887
NlaIII CATG 3 cut(s) 178, 186, 286
NlaIV GGNNCC 3 cut(s) 213, 1042, 1162
NmuCI GTSAC 1 cut(s) 10
NspI RCATGY 1 cut(s) 186
PaeI GCATGC 1 cut(s) 186
PceI AGGCCT 1 cut(s) 503
PctI GAATGC 1 cut(s) 515
PfeI GAWTC 4 cut(s) 349, 674, 777, 836
PflFI GACNNNGTC 1 cut(s) 950
PkrI GCNGC 7 cut(s) 157, 301, 319, 322, 610, 685, 1131
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
Psp6I CCWGG 4 cut(s) 84, 112, 357, 1005
PspGI CCWGG 4 cut(s) 84, 112, 357, 1005
PspN4I GGNNCC 3 cut(s) 213, 1042, 1162
PstI CTGCAG 2 cut(s) 165, 253
PsuI RGATCY 1 cut(s) 1154
PsyI GACNNNGTC 1 cut(s) 950
PvuII CAGCTG 1 cut(s) 1129
RsaI GTAC 5 cut(s) 122, 427, 658, 995, 1151
RsaNI GTAC 5 cut(s) 121, 426, 657, 994, 1150
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 1 cut(s) 1168
SatI GCNGC 7 cut(s) 156, 300, 318, 321, 609, 684, 1130
Sau3AI GATC 3 cut(s) 22, 369, 1154
SchI GAGTC 1 cut(s) 300
ScrFI CCNGG 5 cut(s) 86, 114, 359, 560, 1007
SfaNI GCATC 3 cut(s) 233, 941, 1000
SfcI CTRYAG 4 cut(s) 161, 249, 730, 1124
SmiMI CAYNNNNRTG 1 cut(s) 200
SphI GCATGC 1 cut(s) 186
Sse9I AATT 5 cut(s) 327, 707, 756, 798, 942
SseBI AGGCCT 1 cut(s) 503
SsiI CCGC 1 cut(s) 955
SspMI CTAG 1 cut(s) 888
StuI AGGCCT 1 cut(s) 503
StyD4I CCNGG 5 cut(s) 84, 112, 357, 558, 1005
StyI CCWWGG 1 cut(s) 898
TaaI ACNGT 5 cut(s) 566, 773, 812, 831, 952
TaiI ACGT 1 cut(s) 133
TasI AATT 5 cut(s) 327, 707, 756, 798, 942
TatI WGTACW 1 cut(s) 993
TfiI GAWTC 4 cut(s) 349, 674, 777, 836
Tru1I TTAA 1 cut(s) 1168
Tru9I TTAA 1 cut(s) 1168
TscAI CASTG 5 cut(s) 165, 202, 475, 846, 955
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 7 cut(s) 155, 299, 317, 320, 608, 683, 1129
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 163, 441
TspGWI ACGGA 3 cut(s) 473, 1070, 1167
TspRI CASTG 5 cut(s) 165, 202, 475, 846, 955
Tth111I GACNNNGTC 1 cut(s) 950
XceI RCATGY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 56
XmiI GTMKAC 3 cut(s) 636, 729, 807
XspI CTAG 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.