Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002088

Physical Location & Seq

Genomic Coordinates Forward (+)

6066194 .. 6071966

Gene Structure 5773 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1051700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2817 bp

ATGGCGACTCGTCCCACCGACTCAGCTCCCATAACCCGGAACGTCTCCGAGTCCAGGAAGGCCTGGCACCTCTTCGCCCTCCTCTTACAACTTCGCCGCCCGACTCACCTCTCGGAGCTCGCCTCTCGATGCACATTGTTCCCTGCATCGCCTCGCCTAGTCCGATACCTTTGCTCCACCCCGGGCTCTCCTCTCTGCTTATCGAACCAGCTCTACGTCACGCCGTCACCCGCTTCCTTAGCAGCTTTCCTCGAGTTCTTTTCACATAGCAGTGCCGGCGATGCAGTGAGAGTCTGCTTGGGGAAGCGGAAACGGGCCCTTTGGGATTTCGGATTTGGGCGTCCTGCGGCAAAGAGATTAATTGTTGATTTTGGAAATGGGAATGAAGACAGTAGAGCGCGGAAAGCAGTTGGTCATACTCGTTCGAAGACACATTCTCATATGGCTGACTACTACATCTGCAAATACATAAATATGCTACAAAGTTGGATGACACTGACGGTGAACAAATCGATGTTAAATTCTTCGTTATATATCAATTACAGAACTGGACAATCAAGTTTAGGATTGAATAGTGTTGACCATGTAGAGGATCAAACAGAGGGGACCAATACAATTGTGGAAGTGAAAGTTAACAGACTGGTAATGGATTGTGTCGTCGGTTTAGAACCAGTTCTTCCAATCATGGCATCTGATGATTTAGTCTTTTATGAAGGAGCTGACATACATGAGATAGACTGCAGTGGGATTTTATGTCTGGAAGAAATGAAAGAGAACAATATATCACCAGTCGTGGATGAGAATGTAAATGGTAGTTGTAAAGTTACAAATGTTGGACTCGTTGAAGATGAAAGAGTTGTTGGAGGTGAGGAGGAAGAGTTGTTAGTGGCTTCTCTCATCCCTAATGGAGAAATAGAAAATTTCTCCCAGACAAATATTGCTCTTTATGATATGATGCCTAGGCGTAAGGTCAAAAGATTGAGAAAGGCGGATTGTGAAGATATGATGCCTCTGAATAAAAAGCAAACAAGAAGACATGAGACCAAGACCATAAGTTCAGCTACTGAATTTTCTGTATTGCAAAAGCAATTGTTGATGCCCTCCTCCAAGATGAAGGCGGTTGACAAGGATAAAATGGCTCCGAGACTGCAATCACACAACTTAGAGCATGATCAGGCTGTTGCCACTCCCAAAGAGAACCAGAAGCGTGAAACAAATCAAAAGCCAATCAGCGTAGTACAGAAATCAAAACGGAATTGTGATGACATGCATATTAATGAAAGAAATGGACACCATAAATCTGTTTCTCTTAAGGATCAACCGGAGAAAAAAGAATTTCCAATTTTTGAATCCCATATTGTAGAAGAAGAAGAAGGTTCAGGTGGTTATGGCACTGTTTACAGGGCACGGACCAAAAATGACAACAAAAAGGTCGCCATTAAGTGCCCACATGCTAATGCTCATAAGCATCATGTTAACAATGAGTTAAGGATGCTGGAAAAATTTGGGGGCAAAAACTTCGTGATAAAATATGAAGGCCATATCAAGAATGAAAATTCCAGTTGCTTTGTACTGCAACACGTTGAGCATGATAGGCCTGAGGTTTTAAAGCAAGAGATAGGTCTTTCTCACCTACATTGGTATGGCTATTGCTTGTTTAAGGCGCTGGCAAGCCTTCATAGACAGGGAGTCGTACATAGAGATGTTAAACCTGGAAACTTCCTTTTCTCTCGTAAGGCCAACAAGGGTTACCTCATAGATTTCAATCTTGCAACGGATTTGCATATGAAGCCTGGAACTCGCAAATTAAAATCTGGATATGACTATGATGAGAAACTTACAAATGCAAAATCTGTACCTCAAACCCCACTTCAGAAGTTGCCAACTGCTAAGTCTTTAATAATTGCCAATGGGGAGACCAAAAAAGGTCTCAAATCCACTTTTGATCCGAGGGACTTGAAAAAAAAGGCTCTTAGCCAAATAAAGCCCTACAATTCTTCGGGTAGCCGGAGCCTAATCAAAAGTCAGGGTGCAGATGGCTCAGGTATAACATCAGTAAAGGATGTGAGTAATATCAGGACTCCTCCAGTAGAAAGGTTGAGGGAACCTGTGCCATCCCAAGGAAGGAAAGAGCTCATCAACCTAGTACAGGAAGCAATGCAGAGTCAAAACCATATATCATCAGGTGTTTCGGCTCCTATGAGAAAGAGGATTCCTGCCCCTCCTGGAAATGAGGATGACAAGCTTTTCTATATCACTCCAATGCCTCTGCACTCAACTGGCAATGGTGTTGGTGGTGCAGATTTGATCAGAAGCAGAGGGGGTGGAAAGCAAAAGAAAGAAGGTCCTTGTGCTGGAACTAAAGGATACCGAGCTCCGGAGGTTCTCTTGAGATCCCCATTTCAAGGCCCCAAGCTTGATATCTGGTCTGCCGGTGTCACTCTACTCTACTTCATGATTGGCAGAACACCCTTTGGCGGCGATCCTGAACAGAACATAAAGGACATCGCAAAGTTGAGAGGCAGTGAAGATTTATGGGAAGTTGCCAAGCTACACGACCGCGAATTGTCCTTTCCAGCGGGCCTCTATTGTACAGAATCTTTGCCGTCTATCAATCTGCAGGATTGGTGTAGAAGGAGCACAAAGAGGCCGGATTTCTTCAACGAAATCCCGGGACCGCTTTTTGATCTTGTCGATAAGTGCTTGGCAGTGAACCCGAGGGTTAGGATAAGCGCCGAGGAAGCACTTCGACATGAGTTTTTTGCTCCGTGCCATGAAGAGCTGAGAAAGCTGAGGCTTCAGAGGCAAGTGCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000082 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006950 GO:0006974 GO:0007049 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0008360 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010389 GO:0010556 GO:0010557 GO:0010564 GO:0010571 GO:0010604 GO:0010948 GO:0010971 GO:0015630 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0033262 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045171 GO:0045740 GO:0045786 GO:0045787 GO:0045931 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070316 GO:0070317 GO:0071704 GO:0072686 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0098657 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1903047 GO:2000105 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 map04110 map04111 map04113

Pfam Domains

Protein Families

Pkinase

Protein Analysis

939

Amino Acids

105.32

Weight (kDa)

9.2

Isoelectric Point (pI)

52.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	456 - 597	3.7e-11	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	457 - 599	5.3e-23	Protein kinase domain
Pkinase	PF00069	784 - 921	1.2e-15	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002088)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16970
fragaria_vesca	FvH4_5g20210 FvH4_5g20210 FvH4_7g10370 FvH4_7g10370 FvH4_7g10370 FvH4_7g10370 FvH4_7g10370 FvH4_7g10370 FvH4_7g10370
malus_domestica	MD04G1051700.v1.1 MD06G1048400.v1.1 MD07G1109800.v1.1
prunus_persica	Prupe.5G058600_v2.0.a1 Prupe.5G058600_v2.0.a1
pyrus_communis	pycom04g04540 pycom06g04150 pycom07g09450 pycom07g09460
rosa_chinensis	RchiOBHm_Chr1g0348071 RchiOBHm_Chr7g0205281
rosa_laevigata	RLG00000003391 RLG00000028693
rosa_multiflora	Rmu_co8277205.1_g000001 Rmu_sc0000253.1_g000018 Rmu_sc0001072.1_g000017 Rmu_sc0002523.1_g000002
rosa_roxburghii	Rroxscaffold_3G00252510 Rroxscaffold_4G00307260 Rroxscaffold_4G00307270
rosa_rugosa	Rorug01G0192000 Rorug01G0192100 Rorug07G0089300 Rorug07G0089400.1
rosa_samantha	Rh1AG209800 Rh1BG175400 Rh1CG194200 Rh1DG205200 Rh7AG219800 Rh7BG215900 Rh7CG232700 Rh7DG226800
rosa_wichuraiana	Rw1G017500 Rw7G019010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	66
AccII	CGCG	2 cut(s)	400, 2562
AccIII	TCCGGA	1 cut(s)	2377
AclWI	GGATC	5 cut(s)	600, 1323, 1940, 2388, 2477
AcsI	RAATTY	6 cut(s)	520, 919, 1067, 1334, 1502, 1555
AcuI	CTGAAG	2 cut(s)	1856, 2783
AcyI	GRCGYC	1 cut(s)	340
AfaI	GTAC	6 cut(s)	1239, 1572, 1695, 1857, 2148, 2593
AflII	CTTAAG	1 cut(s)	1310
AflIII	ACRYGT	1 cut(s)	1579
AgsI	TTSAA	7 cut(s)	571, 845, 1349, 1765, 1960, 2405, 2662
AhdI	GACNNNNNGTC	1 cut(s)	1688
AjnI	CCWGG	5 cut(s)	53, 62, 1711, 1792, 2224
AjuI	GAANNNNNNNTTGG	2 cut(s)	843, 875
Alw21I	GWGCWC	4 cut(s)	120, 2136, 2377, 2642
Alw26I	GTCTC	5 cut(s)	49, 1034, 1138, 1910, 1934
AlwI	GGATC	5 cut(s)	600, 1323, 1940, 2388, 2477
AlwNI	CAGNNNCTG	1 cut(s)	1064
Ama87I	CYCGRG	4 cut(s)	181, 251, 2671, 2716
Aor13HI	TCCGGA	1 cut(s)	2377
AoxI	GGCC	8 cut(s)	60, 315, 1537, 1595, 1737, 2407, 2581, 2648
ApaI	GGGCCC	1 cut(s)	319
ApeKI	GCWGC	1 cut(s)	242
ApoI	RAATTY	6 cut(s)	520, 919, 1067, 1334, 1502, 1555
AseI	ATTAAT	2 cut(s)	359, 1275
Asp700I	GAANNNNTTC	2 cut(s)	672, 2745
AspA2I	CCTAGG	1 cut(s)	959
AspLEI	GCGC	3 cut(s)	400, 1666, 2735
AspS9I	GGNCC	8 cut(s)	315, 316, 606, 1410, 2345, 2408, 2581, 2675
AsuC2I	CCSGG	5 cut(s)	37, 182, 183, 2672, 2673
AsuHPI	GGTGA	6 cut(s)	98, 219, 514, 777, 878, 1622
AsuII	TTCGAA	1 cut(s)	425
AvaI	CYCGRG	4 cut(s)	181, 251, 2671, 2716
AvaII	GGWCC	4 cut(s)	606, 1410, 2345, 2675
AvrII	CCTAGG	1 cut(s)	959
AxyI	CCTNAGG	1 cut(s)	1599
BaeGI	GKGCMC	3 cut(s)	319, 1408, 1448
BanI	GGYRCC	1 cut(s)	66
BanII	GRGCYC	5 cut(s)	120, 188, 319, 2136, 2377
BbsI	GAAGAC	3 cut(s)	393, 434, 1039
Bbv12I	GWGCWC	4 cut(s)	120, 2136, 2377, 2642
BbvCI	CCTCAGC	1 cut(s)	2792
BbvI	GCAGC	1 cut(s)	254
BccI	CCATC	2 cut(s)	2030, 2122
BceAI	ACGGC	2 cut(s)	208, 2590
BcgI	CGANNNNNNTGC	4 cut(s)	153, 187, 1501, 1535
BciT130I	CCWGG	5 cut(s)	55, 64, 1713, 1794, 2226
BciVI	GTATCC	1 cut(s)	2360
BclI	TGATCA	2 cut(s)	1171, 2307
BcnI	CCSGG	5 cut(s)	37, 182, 183, 2672, 2673
BcoDI	GTCTC	5 cut(s)	49, 1034, 1138, 1910, 1934
BfaI	CTAG	3 cut(s)	158, 960, 2144
BfmI	CTRYAG	2 cut(s)	739, 2618
BfoI	RGCGCY	2 cut(s)	1667, 2736
BfrI	CTTAAG	1 cut(s)	1310
BfuI	GTATCC	1 cut(s)	2360
BisI	GCNGC	4 cut(s)	97, 243, 348, 2479
BlnI	CCTAGG	1 cut(s)	959
BlsI	GCNGC	4 cut(s)	98, 244, 349, 2480
Bme18I	GGWCC	4 cut(s)	606, 1410, 2345, 2675
BmeRI	GACNNNNNGTC	1 cut(s)	1688
BmeT110I	CYCGRG	4 cut(s)	181, 251, 2671, 2716
BmgT120I	GGNCC	8 cut(s)	315, 316, 606, 1410, 2345, 2408, 2581, 2675
BmiI	GGNNCC	9 cut(s)	68, 317, 607, 1140, 2012, 2106, 2196, 2410, 2676
BmsI	GCATC	9 cut(s)	119, 155, 271, 698, 945, 996, 1086, 1477, 1482
BpiI	GAAGAC	3 cut(s)	393, 434, 1039
BplI	GAGNNNNNCTC	2 cut(s)	107, 139
BpmI	CTGGAG	1 cut(s)	2070
Bpu10I	CCTNAGC	3 cut(s)	238, 2041, 2792
Bpu14I	TTCGAA	1 cut(s)	425
BpuEI	CTTGAG	1 cut(s)	2410
BpuMI	CCSGG	5 cut(s)	37, 182, 183, 2672, 2673
Bsa29I	ATCGAT	1 cut(s)	512
BsaBI	GATNNNNATC	1 cut(s)	1764
BsaHI	GRCGYC	1 cut(s)	340
BsaI	GGTCTC	3 cut(s)	1034, 1910, 1934
BsaJI	CCNNGG	8 cut(s)	180, 181, 959, 1949, 2119, 2671, 2717, 2736
BsaWI	WCCGGW	2 cut(s)	1321, 2377
BsaXI	ACNNNNNCTCC	4 cut(s)	158, 188, 863, 893
Bse118I	RCCGGY	2 cut(s)	275, 2432
Bse1I	ACTGG	7 cut(s)	553, 645, 671, 788, 1560, 2087, 2284
Bse21I	CCTNAGG	1 cut(s)	1599
Bse3DI	GCAATG	2 cut(s)	2163, 2290
Bse8I	GATNNNNATC	1 cut(s)	1764
BseAI	TCCGGA	1 cut(s)	2377
BseBI	CCWGG	5 cut(s)	55, 64, 1713, 1794, 2226
BseCI	ATCGAT	1 cut(s)	512
BseDI	CCNNGG	8 cut(s)	180, 181, 959, 1949, 2119, 2671, 2717, 2736
BseGI	GGATG	7 cut(s)	495, 802, 897, 1497, 2068, 2114, 2242
BseJI	GATNNNNATC	1 cut(s)	1764
BseMI	GCAATG	2 cut(s)	2163, 2290
BseMII	CTCAG	5 cut(s)	36, 1590, 2055, 2774, 2783
BseNI	ACTGG	7 cut(s)	553, 645, 671, 788, 1560, 2087, 2284
BseRI	GAGGAG	5 cut(s)	71, 180, 884, 1093, 2073
BseSI	GKGCMC	3 cut(s)	319, 1408, 1448
BseXI	GCAGC	1 cut(s)	254
BsgI	GTGCAG	3 cut(s)	2052, 2255, 2319
Bsh1236I	CGCG	2 cut(s)	400, 2562
Bsh1285I	CGRYCG	1 cut(s)	2560
BshFI	GGCC	8 cut(s)	62, 317, 1539, 1597, 1739, 2409, 2583, 2650
BshNI	GGYRCC	1 cut(s)	66
BshVI	ATCGAT	1 cut(s)	512
BsiEI	CGRYCG	1 cut(s)	2560
BsiHKAI	GWGCWC	4 cut(s)	120, 2136, 2377, 2642
BsiHKCI	CYCGRG	4 cut(s)	181, 251, 2671, 2716
BsiSI	CCGG	9 cut(s)	37, 182, 276, 1322, 2008, 2378, 2433, 2651, 2672
BslFI	GGGAC	3 cut(s)	619, 1967, 2688
BsmAI	GTCTC	5 cut(s)	49, 1034, 1138, 1910, 1934
BsmBI	CGTCTC	1 cut(s)	49
BsmFI	GGGAC	3 cut(s)	619, 1967, 2688
BsnI	GGCC	8 cut(s)	62, 317, 1539, 1597, 1739, 2409, 2583, 2650
Bso31I	GGTCTC	3 cut(s)	1034, 1910, 1934
BsoBI	CYCGRG	4 cut(s)	181, 251, 2671, 2716
Bsp119I	TTCGAA	1 cut(s)	425
Bsp120I	GGGCCC	1 cut(s)	315
Bsp1286I	GDGCHC	8 cut(s)	120, 188, 319, 1408, 1448, 2136, 2377, 2642
Bsp13I	TCCGGA	1 cut(s)	2377
Bsp1407I	TGTACA	1 cut(s)	2591
Bsp143I	GATC	8 cut(s)	592, 1171, 1315, 1945, 2307, 2393, 2482, 2686
BspANI	GGCC	8 cut(s)	62, 317, 1539, 1597, 1739, 2409, 2583, 2650
BspCNI	CTCAG	5 cut(s)	35, 1591, 2054, 2775, 2784
BspDI	ATCGAT	1 cut(s)	512
BspEI	TCCGGA	1 cut(s)	2377
BspFNI	CGCG	2 cut(s)	400, 2562
BspHI	TCATGA	1 cut(s)	2454
BspLI	GGNNCC	9 cut(s)	68, 317, 607, 1140, 2012, 2106, 2196, 2410, 2676
BspMAI	CTGCAG	2 cut(s)	743, 2622
BspPI	GGATC	5 cut(s)	600, 1323, 1940, 2388, 2477
BspQI	GCTCTTC	1 cut(s)	2772
BspT104I	TTCGAA	1 cut(s)	425
BspT107I	GGYRCC	1 cut(s)	66
BspTI	CTTAAG	1 cut(s)	1310
BspTNI	GGTCTC	3 cut(s)	1034, 1910, 1934
BsrDI	GCAATG	2 cut(s)	2163, 2290
BsrFI	RCCGGY	2 cut(s)	275, 2432
BsrGI	TGTACA	1 cut(s)	2591
BsrI	ACTGG	7 cut(s)	553, 645, 671, 788, 1560, 2087, 2284
BssAI	RCCGGY	2 cut(s)	275, 2432
BssECI	CCNNGG	8 cut(s)	180, 181, 959, 1949, 2119, 2671, 2717, 2736
BssMI	GATC	8 cut(s)	592, 1171, 1315, 1945, 2307, 2393, 2482, 2686
BssNI	GRCGYC	1 cut(s)	340
BssT1I	CCWWGG	2 cut(s)	959, 2119
Bst2UI	CCWGG	5 cut(s)	55, 64, 1713, 1794, 2226
Bst4CI	ACNGT	3 cut(s)	392, 502, 1396
Bst6I	CTCTTC	3 cut(s)	77, 870, 2772
BstACI	GRCGYC	1 cut(s)	340
BstAFI	CTTAAG	1 cut(s)	1310
BstAUI	TGTACA	1 cut(s)	2591
BstBI	TTCGAA	1 cut(s)	425
BstC8I	GCNNGC	5 cut(s)	120, 277, 1668, 1672, 2581
BstDEI	CTNAG	9 cut(s)	22, 238, 1162, 1599, 1890, 1973, 2041, 2783, 2792
BstEII	GGTNACC	1 cut(s)	1748
BstENI	CCTNNNNNAGG	1 cut(s)	2147
BstF5I	GGATG	7 cut(s)	495, 802, 897, 1497, 2068, 2114, 2242
BstFNI	CGCG	2 cut(s)	400, 2562
BstH2I	RGCGCY	2 cut(s)	1667, 2736
BstHHI	GCGC	3 cut(s)	400, 1666, 2735
BstKTI	GATC	8 cut(s)	595, 1174, 1318, 1948, 2310, 2396, 2485, 2689
BstMAI	GTCTC	5 cut(s)	49, 1034, 1138, 1910, 1934
BstMBI	GATC	8 cut(s)	592, 1171, 1315, 1945, 2307, 2393, 2482, 2686
BstMCI	CGRYCG	1 cut(s)	2560
BstMWI	GCNNNNNNNGC	9 cut(s)	239, 276, 281, 404, 1594, 1789, 2741, 2788, 2803
BstNI	CCWGG	5 cut(s)	55, 64, 1713, 1794, 2226
BstNSI	RCATGY	2 cut(s)	1270, 1454
BstPI	GGTNACC	1 cut(s)	1748
BstSFI	CTRYAG	2 cut(s)	739, 2618
BstSLI	GKGCMC	3 cut(s)	319, 1408, 1448
BstUI	CGCG	2 cut(s)	400, 2562
BstV1I	GCAGC	1 cut(s)	254
BstV2I	GAAGAC	3 cut(s)	393, 434, 1039
BstX2I	RGATCY	1 cut(s)	2393
BstYI	RGATCY	1 cut(s)	2393
Bsu15I	ATCGAT	1 cut(s)	512
Bsu36I	CCTNAGG	1 cut(s)	1599
BsuI	GTATCC	1 cut(s)	2360
BsuRI	GGCC	8 cut(s)	62, 317, 1539, 1597, 1739, 2409, 2583, 2650
BsuTUI	ATCGAT	1 cut(s)	512
BtgZI	GCGATG	3 cut(s)	132, 294, 2491
BtsCI	GGATG	7 cut(s)	495, 802, 897, 1497, 2068, 2114, 2242
BtsI	GCAGTG	5 cut(s)	277, 291, 748, 2530, 2715
BtsIMutI	CAGTG	7 cut(s)	277, 291, 494, 748, 1392, 2530, 2715
Cac8I	GCNNGC	5 cut(s)	120, 277, 1668, 1672, 2581
CaiI	CAGNNNCTG	1 cut(s)	1064
CciI	TCATGA	1 cut(s)	2454
CfoI	GCGC	3 cut(s)	400, 1666, 2735
Cfr10I	RCCGGY	2 cut(s)	275, 2432
Cfr13I	GGNCC	8 cut(s)	315, 316, 606, 1410, 2345, 2408, 2581, 2675
Cfr9I	CCCGGG	2 cut(s)	181, 2671
ClaI	ATCGAT	1 cut(s)	512
CseI	GACGC	1 cut(s)	329
Csp6I	GTAC	6 cut(s)	1238, 1571, 1694, 1856, 2147, 2592
CviQI	GTAC	6 cut(s)	1238, 1571, 1694, 1856, 2147, 2592
DdeI	CTNAG	9 cut(s)	22, 238, 1162, 1599, 1890, 1973, 2041, 2783, 2792
DpnI	GATC	8 cut(s)	594, 1173, 1317, 1947, 2309, 2395, 2484, 2688
DpnII	GATC	8 cut(s)	592, 1171, 1315, 1945, 2307, 2393, 2482, 2686
DraI	TTTAAA	1 cut(s)	1608
DriI	GACNNNNNGTC	1 cut(s)	1688
Eam1104I	CTCTTC	3 cut(s)	77, 870, 2772
Eam1105I	GACNNNNNGTC	1 cut(s)	1688
EarI	CTCTTC	3 cut(s)	77, 870, 2772
EciI	GGCGGA	1 cut(s)	1004
Ecl136II	GAGCTC	3 cut(s)	118, 2134, 2375
Eco130I	CCWWGG	2 cut(s)	959, 2119
Eco147I	AGGCCT	2 cut(s)	62, 1597
Eco24I	GRGCYC	5 cut(s)	120, 188, 319, 2136, 2377
Eco31I	GGTCTC	3 cut(s)	1034, 1910, 1934
Eco32I	GATATC	1 cut(s)	2422
Eco47I	GGWCC	4 cut(s)	606, 1410, 2345, 2675
Eco53kI	GAGCTC	3 cut(s)	118, 2134, 2375
Eco57I	CTGAAG	2 cut(s)	1856, 2783
Eco81I	CCTNAGG	1 cut(s)	1599
Eco88I	CYCGRG	4 cut(s)	181, 251, 2671, 2716
Eco91I	GGTNACC	1 cut(s)	1748
EcoICRI	GAGCTC	3 cut(s)	118, 2134, 2375
EcoNI	CCTNNNNNAGG	1 cut(s)	2147
EcoO109I	RGGNCCY	3 cut(s)	316, 2345, 2408
EcoO65I	GGTNACC	1 cut(s)	1748
EcoRII	CCWGG	5 cut(s)	53, 62, 1711, 1792, 2224
EcoRV	GATATC	1 cut(s)	2422
EcoT14I	CCWWGG	2 cut(s)	959, 2119
EcoT22I	ATGCAT	1 cut(s)	1272
EcoT38I	GRGCYC	5 cut(s)	120, 188, 319, 2136, 2377
ErhI	CCWWGG	2 cut(s)	959, 2119
Esp3I	CGTCTC	1 cut(s)	49
FaqI	GGGAC	3 cut(s)	619, 1967, 2688
FauI	CCCGC	2 cut(s)	238, 2572
FauNDI	CATATG	2 cut(s)	441, 1785
FbaI	TGATCA	2 cut(s)	1171, 2307
Fnu4HI	GCNGC	4 cut(s)	97, 243, 348, 2479
FokI	GGATG	7 cut(s)	502, 809, 884, 1504, 2075, 2101, 2249
FriOI	GRGCYC	5 cut(s)	120, 188, 319, 2136, 2377
Fsp4HI	GCNGC	4 cut(s)	97, 243, 348, 2479
FspBI	CTAG	3 cut(s)	158, 960, 2144
GlaI	GCGC	3 cut(s)	399, 1665, 2734
GluI	GCNGC	4 cut(s)	97, 243, 348, 2479
GsuI	CTGGAG	1 cut(s)	2070
HaeII	RGCGCY	2 cut(s)	1667, 2736
HaeIII	GGCC	8 cut(s)	62, 317, 1539, 1597, 1739, 2409, 2583, 2650
HapII	CCGG	9 cut(s)	37, 182, 276, 1322, 2008, 2378, 2433, 2651, 2672
HgaI	GACGC	1 cut(s)	329
HhaI	GCGC	3 cut(s)	400, 1666, 2735
Hin1I	GRCGYC	1 cut(s)	340
Hin6I	GCGC	3 cut(s)	398, 1664, 2733
HinP1I	GCGC	3 cut(s)	398, 1664, 2733
HincII	GTYRAC	4 cut(s)	580, 634, 1123, 1477
HindII	GTYRAC	4 cut(s)	580, 634, 1123, 1477
HindIII	AAGCTT	2 cut(s)	2243, 2414
HpaI	GTTAAC	2 cut(s)	634, 1477
HpaII	CCGG	9 cut(s)	37, 182, 276, 1322, 2008, 2378, 2433, 2651, 2672
HphI	GGTGA	6 cut(s)	98, 219, 514, 777, 878, 1622
Hpy166II	GTNNAC	7 cut(s)	505, 580, 634, 1123, 1399, 1477, 2713
Hpy8I	GTNNAC	7 cut(s)	505, 580, 634, 1123, 1399, 1477, 2713
Hpy99I	CGWCG	1 cut(s)	662
HpyAV	CCTTC	9 cut(s)	52, 707, 1108, 1367, 1529, 1685, 2118, 2336, 2628
HpyCH4III	ACNGT	3 cut(s)	392, 502, 1396
HpyCH4IV	ACGT	3 cut(s)	42, 216, 1581
HpyF10VI	GCNNNNNNNGC	9 cut(s)	239, 276, 281, 404, 1594, 1789, 2741, 2788, 2803
HpyF3I	CTNAG	9 cut(s)	22, 238, 1162, 1599, 1890, 1973, 2041, 2783, 2792
HpySE526I	ACGT	3 cut(s)	42, 216, 1581
Hsp92I	GRCGYC	1 cut(s)	340
HspAI	GCGC	3 cut(s)	398, 1664, 2733
Kpn2I	TCCGGA	1 cut(s)	2377
KroI	GCCGGC	1 cut(s)	275
KroNI	GCCGGC	1 cut(s)	277
Ksp22I	TGATCA	2 cut(s)	1171, 2307
KspAI	GTTAAC	2 cut(s)	634, 1477
Kzo9I	GATC	8 cut(s)	592, 1171, 1315, 1945, 2307, 2393, 2482, 2686
LguI	GCTCTTC	1 cut(s)	2772
Lsp1109I	GCAGC	1 cut(s)	254
LweI	GCATC	9 cut(s)	119, 155, 271, 698, 945, 996, 1086, 1477, 1482
MaeI	CTAG	3 cut(s)	158, 960, 2144
MaeII	ACGT	3 cut(s)	42, 216, 1581
MaeIII	GTNAC	5 cut(s)	217, 225, 823, 1748, 2437
MalI	GATC	8 cut(s)	594, 1173, 1317, 1947, 2309, 2395, 2484, 2688
MboI	GATC	8 cut(s)	592, 1171, 1315, 1945, 2307, 2393, 2482, 2686
MfeI	CAATTG	2 cut(s)	615, 1088
MflI	RGATCY	1 cut(s)	2393
MhlI	GDGCHC	8 cut(s)	120, 188, 319, 1408, 1448, 2136, 2377, 2642
MlyI	GAGTC	8 cut(s)	14, 59, 97, 300, 831, 1698, 2074, 2173
MmeI	TCCRAC	3 cut(s)	467, 814, 841
Mph1103I	ATGCAT	1 cut(s)	1272
MroI	TCCGGA	1 cut(s)	2377
MroNI	GCCGGC	1 cut(s)	275
MroXI	GAANNNNTTC	2 cut(s)	672, 2745
MslI	CAYNNNNRTG	7 cut(s)	270, 473, 1275, 1455, 1641, 1701, 2261
MspA1I	CMGCKG	1 cut(s)	2579
MspCI	CTTAAG	1 cut(s)	1310
MspI	CCGG	9 cut(s)	37, 182, 276, 1322, 2008, 2378, 2433, 2651, 2672
MunI	CAATTG	2 cut(s)	615, 1088
MvaI	CCWGG	5 cut(s)	55, 64, 1713, 1794, 2226
MvnI	CGCG	2 cut(s)	400, 2562
MwoI	GCNNNNNNNGC	9 cut(s)	239, 276, 281, 404, 1594, 1789, 2741, 2788, 2803
NaeI	GCCGGC	1 cut(s)	277
NciI	CCSGG	5 cut(s)	37, 182, 183, 2672, 2673
NdeI	CATATG	2 cut(s)	441, 1785
NdeII	GATC	8 cut(s)	592, 1171, 1315, 1945, 2307, 2393, 2482, 2686
NgoMIV	GCCGGC	1 cut(s)	275
NlaIV	GGNNCC	9 cut(s)	68, 317, 607, 1140, 2012, 2106, 2196, 2410, 2676
NmeAIII	GCCGAG	1 cut(s)	2761
NmuCI	GTSAC	3 cut(s)	217, 225, 2437
NsiI	ATGCAT	1 cut(s)	1272
NspI	RCATGY	2 cut(s)	1270, 1454
NspV	TTCGAA	1 cut(s)	425
PaeR7I	CTCGAG	1 cut(s)	251
PagI	TCATGA	1 cut(s)	2454
PceI	AGGCCT	2 cut(s)	62, 1597
PciSI	GCTCTTC	1 cut(s)	2772
PcsI	WCGNNNNNNNCGW	1 cut(s)	160
PdiI	GCCGGC	1 cut(s)	277
PdmI	GAANNNNTTC	2 cut(s)	672, 2745
PfeI	GAWTC	3 cut(s)	1349, 2212, 2597
PfoI	TCCNGGA	2 cut(s)	53, 2224
PkrI	GCNGC	4 cut(s)	98, 244, 349, 2480
PleI	GAGTC	8 cut(s)	14, 58, 97, 299, 831, 1697, 2074, 2172
PpsI	GAGTC	8 cut(s)	14, 58, 97, 299, 831, 1697, 2074, 2172
PpuMI	RGGWCCY	1 cut(s)	2345
PshBI	ATTAAT	2 cut(s)	359, 1275
Psp124BI	GAGCTC	3 cut(s)	120, 2136, 2377
Psp5II	RGGWCCY	1 cut(s)	2345
Psp6I	CCWGG	5 cut(s)	53, 62, 1711, 1792, 2224
PspEI	GGTNACC	1 cut(s)	1748
PspGI	CCWGG	5 cut(s)	53, 62, 1711, 1792, 2224
PspN4I	GGNNCC	9 cut(s)	68, 317, 607, 1140, 2012, 2106, 2196, 2410, 2676
PspOMI	GGGCCC	1 cut(s)	315
PspPI	GGNCC	8 cut(s)	315, 316, 606, 1410, 2345, 2408, 2581, 2675
PspPPI	RGGWCCY	1 cut(s)	2345
PspXI	VCTCGAGB	1 cut(s)	251
PsrI	GAACNNNNNNTAC	2 cut(s)	197, 229
PstI	CTGCAG	2 cut(s)	743, 2622
PstNI	CAGNNNCTG	1 cut(s)	1064
PsuI	RGATCY	1 cut(s)	2393
RsaI	GTAC	6 cut(s)	1239, 1572, 1695, 1857, 2148, 2593
RsaNI	GTAC	6 cut(s)	1238, 1571, 1694, 1856, 2147, 2592
RseI	CAYNNNNRTG	7 cut(s)	270, 473, 1275, 1455, 1641, 1701, 2261
SacI	GAGCTC	3 cut(s)	120, 2136, 2377
SapI	GCTCTTC	1 cut(s)	2772
SatI	GCNGC	4 cut(s)	97, 243, 348, 2479
Sau3AI	GATC	8 cut(s)	592, 1171, 1315, 1945, 2307, 2393, 2482, 2686
Sau96I	GGNCC	8 cut(s)	315, 316, 606, 1410, 2345, 2408, 2581, 2675
SchI	GAGTC	8 cut(s)	14, 59, 97, 300, 831, 1698, 2074, 2173
SduI	GDGCHC	8 cut(s)	120, 188, 319, 1408, 1448, 2136, 2377, 2642
SfaNI	GCATC	9 cut(s)	119, 155, 271, 698, 945, 996, 1086, 1477, 1482
SfcI	CTRYAG	2 cut(s)	739, 2618
Sfr274I	CTCGAG	1 cut(s)	251
SfuI	TTCGAA	1 cut(s)	425
SinI	GGWCC	4 cut(s)	606, 1410, 2345, 2675
SlaI	CTCGAG	1 cut(s)	251
SmaI	CCCGGG	2 cut(s)	183, 2673
SmiMI	CAYNNNNRTG	7 cut(s)	270, 473, 1275, 1455, 1641, 1701, 2261
SmlI	CTYRAG	3 cut(s)	251, 1310, 2389
SmoI	CTYRAG	3 cut(s)	251, 1310, 2389
SseBI	AGGCCT	2 cut(s)	62, 1597
SspI	AATATT	1 cut(s)	937
SspMI	CTAG	3 cut(s)	158, 960, 2144
SstI	GAGCTC	3 cut(s)	120, 2136, 2377
StuI	AGGCCT	2 cut(s)	62, 1597
StyI	CCWWGG	2 cut(s)	959, 2119
TaaI	ACNGT	3 cut(s)	392, 502, 1396
TaiI	ACGT	3 cut(s)	45, 219, 1584
TaqI	TCGA	7 cut(s)	127, 203, 252, 425, 512, 2694, 2749
TatI	WGTACW	4 cut(s)	1237, 1570, 2146, 2591
TauI	GCSGC	3 cut(s)	99, 350, 2481
TfiI	GAWTC	3 cut(s)	1349, 2212, 2597
TscAI	CASTG	7 cut(s)	277, 291, 501, 748, 1399, 2530, 2715
TseFI	GTSAC	3 cut(s)	217, 225, 2437
TseI	GCWGC	1 cut(s)	242
Tsp45I	GTSAC	3 cut(s)	217, 225, 2437
TspGWI	ACGGA	4 cut(s)	1267, 1423, 1790, 2757
TspMI	CCCGGG	2 cut(s)	181, 2671
TspRI	CASTG	7 cut(s)	277, 291, 501, 748, 1399, 2530, 2715
Vha464I	CTTAAG	1 cut(s)	1310
VpaK11BI	GGWCC	4 cut(s)	606, 1410, 2345, 2675
VspI	ATTAAT	2 cut(s)	359, 1275
XagI	CCTNNNNNAGG	1 cut(s)	2147
XapI	RAATTY	6 cut(s)	520, 919, 1067, 1334, 1502, 1555
XceI	RCATGY	2 cut(s)	1270, 1454
XcmI	CCANNNNNNNNNTGG	1 cut(s)	616
XhoI	CTCGAG	1 cut(s)	251
XmaI	CCCGGG	2 cut(s)	181, 2671
XmaJI	CCTAGG	1 cut(s)	959
XmnI	GAANNNNTTC	2 cut(s)	672, 2745
XspI	CTAG	3 cut(s)	158, 960, 2144
Zsp2I	ATGCAT	1 cut(s)	1272

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.