pycom04g04540

serine threonine-protein kinase cdc7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002088
Physical Location & Seq
Forward (+)
4161574 .. 4167537
5964 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g04540.10

Sequence Viewer

Length: 2691 bp
ATGGAAATCTTTGAAAGTTTGACGTTACGGAGGGGTAAAAATGGCGGGAACTCTTCAGGTGCTAAATCTCAAACCCTACACGTGCGAAATTTGGCGCCCATCTGTCCCCTTCTATTCCAATCTCCCTCTCTCCGTCTCCGATCCAAATCTCATCAATCAACCGTGGAAATGGCGACTCGTCCCACCGACTCAACTCCCATAACCCCAACCGTCTCCGAGTCCGAGAAGGCCTGGCACCTCTTCGCCCTCCTCTTACAACTTCGCCGCCCAACTCACCCCTCCGAGCTCGCCTCTCGATGCACATTGTTCCCCGCATCGCCTCACCTAGTCCGATACCTTTGCTCCACCCCGGGCTCTCCTCTCTGCTTATCGAACCAGCTCTACGTCACGCCGTCTCCGGCTTCCTTAGCAGCTTTCCTCGAGTTCTTTTCACATCGCAGTGCCGGCGATGCAGTGAGAGTCTGCTTGGGGAAGCGGAAACGGGTCGTTTGGGATTCCGGAGTCGGGCCTCCGGCAGCAAAGAGATTAATTGTGGATTTTGGAAATGAGGATCAAACGGAGGGGACCAATACAATTGTGGAAACGAAGGTTAACAGGCTGGTAATGGATTGTGTCATTGGTTTAGAATCAGTTCTTCCAATCATGGCGTCTGATGATTTAGTCTTTTATGAAGGAGCTGACATACATGAGATAGACTGCAGTGGGAATTTGTCTCTGGAAGAAATGAAAGAGAACAATATATCACCAGTCGTGGATGAGAATGTAAATGGTAGTTGTAAAGTTACAAATGTTGGACTCGTTGAAGATGAAAGAGTTGTTGGAGGTGAGGAGGAAGAGTTGTTAGTGGCTTCTCGCAGCCCTAATGGAGAAATAGAAAATTTCTCCCAGACAAATTTTGCTCTTTATGATATGCTGCCTAGGCGTAAGGTCAAAAGATTGAGAAAGGCGGATTGTGAAGATATGATGCCTCTGAATAAAAAGCAAACAAGAAGACATGAGACCAAGACCATAAGTTCAGCTACTGAATTTTCTGTATTGCAAAAGCAATTGTTGATGCCCTCCTCCAAGATGAAGGCGGTTGACAAGGATAAAATGGCTCCGAGACTACAATCACACAACTTAGAGCATGATCAGGCTGTTGCCACTCCCAAAGAGAACCAGAAGTGTGAAACAAATCAAAAGCCAATCATCTTAGTACAGAAATCAAAAAGGAATTGTGATGACGTGCATGTTAATGAAAGAAATGGACGCCATAAATCTGTGTCTCTTAAGGATCAACTGGAGAAAAAAGAATTTCCAATTTTTGAATCCTATATTGTAGAAGAAGAAGAAGGTTCAGGTGGTTATGGCACTGTTTACAGGGCACGGACCAAAAATGACGAGAAAAAGGTCGCCATTAAGTGCCCGCATGCTAATGCTCATAAGAATCATGTTAACAATGAGTTAAGGATGCTGGAAAAGTTTGGGGGCAAAAACTTCGTGATAAAATATGAAGGCCGTATCAAGAATGAAAATTCCAGTTGCTTTGTACTGCAGCACGTTGAGCATGATAGGCCTGAGGTTTTAAAGCAAGAGATAGGTCTTTCTCACCTACATTGGTATGGCTATTGCTTGTTTAAGGCGCTGGCAAGCCTTCATAGACAGAAAGAATGGAAAATTGATGAAGCTAATTTTTCGTATTTCACAGGCAAATTAAAATCTGGATATGACTATGATGAGAAACTTACAAATGCAAAATCTGTACCTCGAACCCCACTTCAGAAGTTGCCAACTGCTAAGTCCTTAATAATTGCCAATGGGGAGACCAAAAAAGGTCTCAAATCCACTTTTGATCCGAGGGACTTGAAAAAAAAGGCTCTTAGCCAAATAAAGCCCTACAATTCTTCGGGTGGCCGGAGCCTAATCAAAAGTCAGGGTGCAGATGGCTCAGGTATAACATCAGTAAAGGATGTGAGTAATATCAGGACTCCTCCAGTAGAAAGGTTGAGGGAACCTGTGCCATCCCAAGGAAGGAAGGAGCTCATCAACCTAGTACAGGAAGCAATGCAGAATCAAAACCATATATCATCAGGTGTTTCGGCTCCTATGAGAAAGAGGATTCCTGCCCCTCCTGGAAATGAGGATGACAAGCTTTTCTATATCACTCCAATGCCTCTGCACTCAACTGGCAATGGTGTTGGCGGTGCAGATTTGATCAGAAGCAGAGGGGGTGGAAAGCAAAAGAAAGAAGGTCCTTGTGCTGGAACTAAAGGATACCGAGCTCCGGAGGTTCTCTTGAGATCCCCATATCAAGGCCCCAAGCTTGATATCTGGTCTGCCGGTGTCACTCTACTCTACTTCATGATTGGCAGAACGCCCTTTGGTGGCGATCCTGAACAGAACATAAAGGACATCGCAAAGTTGAGAGGCAGCGAAGATTTATGGGAAGTTGCCAAGCTACACGACCGCGAATCGTCATTTCCAGCGGTATTAATCATTCATTACTTCTTCGATCTAGATTGGTGTAGAAGGAGCACAAAGAGGCCGGATTTCTACAACGAAATCCCTAGACCGCTTTTTGATCTTGTCGATAAGTGCTTGGCAGTGAACCCGAGGGTTAGGATAAGCGCCGAGGAAGCACTTCGACATGAGTTTTTTGCTCCGTGCCATGAAGAGCTGAGAAAGCTGAGGCTTCAGAGGCAGGTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006950 GO:0006974 GO:0007049 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0008360 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010389 GO:0010556 GO:0010557 GO:0010564 GO:0010571 GO:0010604 GO:0010948 GO:0010971 GO:0015630 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0033262 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045171 GO:0045740 GO:0045786 GO:0045787 GO:0045931 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070316 GO:0070317 GO:0071704 GO:0072686 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0098657 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1903047 GO:2000105 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 map04110 map04111 map04113
Pfam Domains
Protein Families
Pkinase

Protein Analysis

897

Amino Acids

100.66

Weight (kDa)

9.09

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2671
Acc36I ACCTGC 1 cut(s) 2671
AccB1I GGYRCC 2 cut(s) 94, 234
AccII CGCG 1 cut(s) 2448
AccIII TCCGGA 2 cut(s) 497, 2263
AclWI GGATC 6 cut(s) 135, 558, 1281, 1826, 2274, 2363
AcoI YGGCCR 1 cut(s) 1891
AcsI RAATTY 7 cut(s) 88, 706, 877, 892, 1025, 1292, 1513
AcuI CTGAAG 3 cut(s) 39, 1742, 2657
AcvI CACGTG 1 cut(s) 82
AcyI GRCGYC 3 cut(s) 95, 647, 1249
AfaI GTAC 4 cut(s) 1197, 1530, 1743, 2034
AfiI CCNNNNNNNGG 8 cut(s) 504, 2006, 2010, 2035, 2240, 2263, 2291, 2363
AflII CTTAAG 1 cut(s) 1268
AflIII ACRYGT 1 cut(s) 79
AgsI TTSAA 4 cut(s) 14, 803, 1307, 1846
AjiI CACGTC 1 cut(s) 1225
AjnI CCWGG 2 cut(s) 230, 2110
AjuI GAANNNNNNNTTGG 2 cut(s) 801, 833
Alw21I GWGCWC 4 cut(s) 288, 2022, 2263, 2516
Alw26I GTCTC 9 cut(s) 140, 217, 399, 717, 992, 1096, 1269, 1796, 1820
AlwI GGATC 6 cut(s) 135, 558, 1281, 1826, 2274, 2363
AlwNI CAGNNNCTG 2 cut(s) 1022, 2686
Ama87I CYCGRG 3 cut(s) 349, 419, 2590
Aor13HI TCCGGA 2 cut(s) 497, 2263
AoxI GGCC 7 cut(s) 228, 506, 1495, 1553, 1891, 2293, 2522
ApeKI GCWGC 6 cut(s) 410, 515, 855, 913, 1534, 2409
ApoI RAATTY 7 cut(s) 88, 706, 877, 892, 1025, 1292, 1513
AseI ATTAAT 2 cut(s) 527, 2471
Asp700I GAANNNNTTC 2 cut(s) 630, 2619
AspA2I CCTAGG 1 cut(s) 917
AspLEI GCGC 3 cut(s) 97, 1624, 2609
AspS9I GGNCC 5 cut(s) 506, 564, 1368, 2231, 2294
AsuC2I CCSGG 2 cut(s) 350, 351
AsuHPI GGTGA 5 cut(s) 266, 314, 735, 836, 1580
AvaI CYCGRG 3 cut(s) 349, 419, 2590
AvaII GGWCC 3 cut(s) 564, 1368, 2231
AvrII CCTAGG 1 cut(s) 917
AxyI CCTNAGG 1 cut(s) 1557
BaeGI GKGCMC 2 cut(s) 1366, 1406
BanI GGYRCC 2 cut(s) 94, 234
BanII GRGCYC 4 cut(s) 288, 356, 2022, 2263
BbrPI CACGTG 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 997
Bbv12I GWGCWC 4 cut(s) 288, 2022, 2263, 2516
BbvCI CCTCAGC 1 cut(s) 2666
BbvI GCAGC 6 cut(s) 422, 527, 867, 900, 1546, 2421
BccI CCATC 3 cut(s) 107, 1916, 2008
BceAI ACGGC 2 cut(s) 376, 1482
BcgI CGANNNNNNTGC 4 cut(s) 321, 355, 1459, 1493
BciT130I CCWGG 2 cut(s) 232, 2112
BciVI GTATCC 1 cut(s) 2246
BclI TGATCA 2 cut(s) 1129, 2193
BcnI CCSGG 2 cut(s) 350, 351
BcoDI GTCTC 9 cut(s) 140, 217, 399, 717, 992, 1096, 1269, 1796, 1820
BfaI CTAG 5 cut(s) 326, 918, 2030, 2495, 2547
BfmI CTRYAG 2 cut(s) 697, 1532
BfoI RGCGCY 3 cut(s) 98, 1625, 2610
BfrI CTTAAG 1 cut(s) 1268
BfuAI ACCTGC 1 cut(s) 2671
BfuI GTATCC 1 cut(s) 2246
BisI GCNGC 7 cut(s) 265, 411, 516, 856, 914, 1535, 2410
BlnI CCTAGG 1 cut(s) 917
BlsI GCNGC 7 cut(s) 266, 412, 517, 857, 915, 1536, 2411
Bme1390I CCNGG 4 cut(s) 232, 350, 351, 2112
Bme18I GGWCC 3 cut(s) 564, 1368, 2231
BmeT110I CYCGRG 3 cut(s) 349, 419, 2590
BmgBI CACGTC 1 cut(s) 1225
BmgT120I GGNCC 5 cut(s) 506, 564, 1368, 2231, 2294
BmiI GGNNCC 8 cut(s) 96, 236, 565, 1098, 1898, 1992, 2082, 2296
BmrFI CCNGG 4 cut(s) 232, 350, 351, 2112
BmsI GCATC 6 cut(s) 287, 323, 439, 954, 1044, 1440
BpiI GAAGAC 1 cut(s) 997
BplI GAGNNNNNCTC 2 cut(s) 275, 307
BpmI CTGGAG 2 cut(s) 1301, 1956
Bpu10I CCTNAGC 3 cut(s) 406, 1927, 2666
BpuEI CTTGAG 1 cut(s) 2296
BpuMI CCSGG 2 cut(s) 350, 351
BsaAI YACGTR 1 cut(s) 82
BsaBI GATNNNNATC 1 cut(s) 145
BsaHI GRCGYC 3 cut(s) 95, 647, 1249
BsaI GGTCTC 3 cut(s) 992, 1796, 1820
BsaJI CCNNGG 8 cut(s) 162, 348, 349, 917, 1835, 2005, 2591, 2610
BsaWI WCCGGW 2 cut(s) 497, 2263
BsaXI ACNNNNNCTCC 6 cut(s) 326, 356, 379, 409, 821, 851
Bsc4I CCNNNNNNNGG 8 cut(s) 504, 2006, 2010, 2035, 2240, 2263, 2291, 2363
Bse118I RCCGGY 2 cut(s) 443, 2318
Bse1I ACTGG 5 cut(s) 746, 1284, 1518, 1973, 2170
Bse21I CCTNAGG 1 cut(s) 1557
Bse3DI GCAATG 2 cut(s) 2049, 2176
Bse8I GATNNNNATC 1 cut(s) 145
BseAI TCCGGA 2 cut(s) 497, 2263
BseBI CCWGG 2 cut(s) 232, 2112
BseDI CCNNGG 8 cut(s) 162, 348, 349, 917, 1835, 2005, 2591, 2610
BseGI GGATG 5 cut(s) 760, 1455, 1954, 2000, 2128
BseJI GATNNNNATC 1 cut(s) 145
BseLI CCNNNNNNNGG 8 cut(s) 504, 2006, 2010, 2035, 2240, 2263, 2291, 2363
BseMI GCAATG 2 cut(s) 2049, 2176
BseMII CTCAG 4 cut(s) 1548, 1941, 2648, 2657
BseNI ACTGG 5 cut(s) 746, 1284, 1518, 1973, 2170
BseRI GAGGAG 5 cut(s) 239, 348, 842, 1051, 1959
BseSI GKGCMC 2 cut(s) 1366, 1406
BseXI GCAGC 6 cut(s) 422, 527, 867, 900, 1546, 2421
BsgI GTGCAG 3 cut(s) 1938, 2141, 2205
Bsh1236I CGCG 1 cut(s) 2448
Bsh1285I CGRYCG 1 cut(s) 2446
BshFI GGCC 7 cut(s) 230, 508, 1497, 1555, 1893, 2295, 2524
BshNI GGYRCC 2 cut(s) 94, 234
BsiEI CGRYCG 1 cut(s) 2446
BsiHKAI GWGCWC 4 cut(s) 288, 2022, 2263, 2516
BsiHKCI CYCGRG 3 cut(s) 349, 419, 2590
BsiSI CCGG 9 cut(s) 350, 398, 444, 498, 512, 1894, 2264, 2319, 2525
BslFI GGGAC 4 cut(s) 90, 165, 577, 1853
BslI CCNNNNNNNGG 8 cut(s) 504, 2006, 2010, 2035, 2240, 2263, 2291, 2363
BsmAI GTCTC 9 cut(s) 140, 217, 399, 717, 992, 1096, 1269, 1796, 1820
BsmBI CGTCTC 3 cut(s) 140, 217, 399
BsmFI GGGAC 4 cut(s) 90, 165, 577, 1853
BsnI GGCC 7 cut(s) 230, 508, 1497, 1555, 1893, 2295, 2524
Bso31I GGTCTC 3 cut(s) 992, 1796, 1820
BsoBI CYCGRG 3 cut(s) 349, 419, 2590
Bsp1286I GDGCHC 7 cut(s) 288, 356, 1366, 1406, 2022, 2263, 2516
Bsp13I TCCGGA 2 cut(s) 497, 2263
BspANI GGCC 7 cut(s) 230, 508, 1497, 1555, 1893, 2295, 2524
BspCNI CTCAG 4 cut(s) 1549, 1940, 2649, 2658
BspEI TCCGGA 2 cut(s) 497, 2263
BspFNI CGCG 1 cut(s) 2448
BspHI TCATGA 1 cut(s) 2340
BspLI GGNNCC 8 cut(s) 96, 236, 565, 1098, 1898, 1992, 2082, 2296
BspMAI CTGCAG 2 cut(s) 701, 1536
BspMI ACCTGC 1 cut(s) 2671
BspPI GGATC 6 cut(s) 135, 558, 1281, 1826, 2274, 2363
BspQI GCTCTTC 1 cut(s) 2646
BspT107I GGYRCC 2 cut(s) 94, 234
BspTI CTTAAG 1 cut(s) 1268
BspTNI GGTCTC 3 cut(s) 992, 1796, 1820
BsrDI GCAATG 2 cut(s) 2049, 2176
BsrFI RCCGGY 2 cut(s) 443, 2318
BsrI ACTGG 5 cut(s) 746, 1284, 1518, 1973, 2170
BssAI RCCGGY 2 cut(s) 443, 2318
BssECI CCNNGG 8 cut(s) 162, 348, 349, 917, 1835, 2005, 2591, 2610
BssNI GRCGYC 3 cut(s) 95, 647, 1249
BssT1I CCWWGG 2 cut(s) 917, 2005
Bst2UI CCWGG 2 cut(s) 232, 2112
Bst4CI ACNGT 3 cut(s) 163, 211, 1354
Bst6I CTCTTC 4 cut(s) 58, 245, 828, 2646
BstACI GRCGYC 3 cut(s) 95, 647, 1249
BstAFI CTTAAG 1 cut(s) 1268
BstBAI YACGTR 1 cut(s) 82
BstC8I GCNNGC 6 cut(s) 288, 445, 1406, 1410, 1626, 1630
BstDEI CTNAG 9 cut(s) 406, 1120, 1192, 1557, 1776, 1859, 1927, 2657, 2666
BstDSI CCRYGG 1 cut(s) 162
BstENI CCTNNNNNAGG 1 cut(s) 2033
BstF5I GGATG 5 cut(s) 760, 1455, 1954, 2000, 2128
BstFNI CGCG 1 cut(s) 2448
BstH2I RGCGCY 3 cut(s) 98, 1625, 2610
BstHHI GCGC 3 cut(s) 97, 1624, 2609
BstMAI GTCTC 9 cut(s) 140, 217, 399, 717, 992, 1096, 1269, 1796, 1820
BstMCI CGRYCG 1 cut(s) 2446
BstMWI GCNNNNNNNGC 9 cut(s) 407, 444, 449, 919, 1543, 1552, 2615, 2662, 2677
BstNI CCWGG 2 cut(s) 232, 2112
BstNSI RCATGY 2 cut(s) 1232, 1412
BstSCI CCNGG 4 cut(s) 230, 348, 349, 2110
BstSFI CTRYAG 2 cut(s) 697, 1532
BstSLI GKGCMC 2 cut(s) 1366, 1406
BstUI CGCG 1 cut(s) 2448
BstV1I GCAGC 6 cut(s) 422, 527, 867, 900, 1546, 2421
BstV2I GAAGAC 1 cut(s) 997
BstX2I RGATCY 1 cut(s) 2279
BstYI RGATCY 1 cut(s) 2279
Bsu36I CCTNAGG 1 cut(s) 1557
BsuI GTATCC 1 cut(s) 2246
BsuRI GGCC 7 cut(s) 230, 508, 1497, 1555, 1893, 2295, 2524
BtgI CCRYGG 1 cut(s) 162
BtgZI GCGATG 4 cut(s) 300, 419, 462, 2377
BtrI CACGTC 1 cut(s) 1225
BtsCI GGATG 5 cut(s) 760, 1455, 1954, 2000, 2128
BtsI GCAGTG 4 cut(s) 445, 459, 706, 2589
BtsIMutI CAGTG 5 cut(s) 445, 459, 706, 1350, 2589
BveI ACCTGC 1 cut(s) 2671
Cac8I GCNNGC 6 cut(s) 288, 445, 1406, 1410, 1626, 1630
CaiI CAGNNNCTG 2 cut(s) 1022, 2686
CciI TCATGA 1 cut(s) 2340
CfoI GCGC 3 cut(s) 97, 1624, 2609
Cfr10I RCCGGY 2 cut(s) 443, 2318
Cfr13I GGNCC 5 cut(s) 506, 564, 1368, 2231, 2294
Cfr9I CCCGGG 1 cut(s) 349
CseI GACGC 2 cut(s) 636, 1257
Csp6I GTAC 4 cut(s) 1196, 1529, 1742, 2033
CviQI GTAC 4 cut(s) 1196, 1529, 1742, 2033
DdeI CTNAG 9 cut(s) 406, 1120, 1192, 1557, 1776, 1859, 1927, 2657, 2666
DinI GGCGCC 1 cut(s) 96
DraI TTTAAA 1 cut(s) 1566
EaeI YGGCCR 1 cut(s) 1891
Eam1104I CTCTTC 4 cut(s) 58, 245, 828, 2646
EarI CTCTTC 4 cut(s) 58, 245, 828, 2646
EciI GGCGGA 1 cut(s) 962
Ecl136II GAGCTC 3 cut(s) 286, 2020, 2261
Eco130I CCWWGG 2 cut(s) 917, 2005
Eco147I AGGCCT 2 cut(s) 230, 1555
Eco24I GRGCYC 4 cut(s) 288, 356, 2022, 2263
Eco31I GGTCTC 3 cut(s) 992, 1796, 1820
Eco32I GATATC 1 cut(s) 2308
Eco47I GGWCC 3 cut(s) 564, 1368, 2231
Eco53kI GAGCTC 3 cut(s) 286, 2020, 2261
Eco57I CTGAAG 3 cut(s) 39, 1742, 2657
Eco72I CACGTG 1 cut(s) 82
Eco81I CCTNAGG 1 cut(s) 1557
Eco88I CYCGRG 3 cut(s) 349, 419, 2590
EcoICRI GAGCTC 3 cut(s) 286, 2020, 2261
EcoNI CCTNNNNNAGG 1 cut(s) 2033
EcoO109I RGGNCCY 2 cut(s) 2231, 2294
EcoRII CCWGG 2 cut(s) 230, 2110
EcoRV GATATC 1 cut(s) 2308
EcoT14I CCWWGG 2 cut(s) 917, 2005
EcoT38I GRGCYC 4 cut(s) 288, 356, 2022, 2263
EgeI GGCGCC 1 cut(s) 96
EheI GGCGCC 1 cut(s) 96
ErhI CCWWGG 2 cut(s) 917, 2005
Esp3I CGTCTC 3 cut(s) 140, 217, 399
FaqI GGGAC 4 cut(s) 90, 165, 577, 1853
FauI CCCGC 3 cut(s) 38, 319, 1413
FbaI TGATCA 2 cut(s) 1129, 2193
Fnu4HI GCNGC 7 cut(s) 265, 411, 516, 856, 914, 1535, 2410
FokI GGATG 5 cut(s) 767, 1462, 1961, 1987, 2135
FriOI GRGCYC 4 cut(s) 288, 356, 2022, 2263
Fsp4HI GCNGC 7 cut(s) 265, 411, 516, 856, 914, 1535, 2410
FspBI CTAG 5 cut(s) 326, 918, 2030, 2495, 2547
GlaI GCGC 3 cut(s) 96, 1623, 2608
GluI GCNGC 7 cut(s) 265, 411, 516, 856, 914, 1535, 2410
GsuI CTGGAG 2 cut(s) 1301, 1956
HaeII RGCGCY 3 cut(s) 98, 1625, 2610
HaeIII GGCC 7 cut(s) 230, 508, 1497, 1555, 1893, 2295, 2524
HapII CCGG 9 cut(s) 350, 398, 444, 498, 512, 1894, 2264, 2319, 2525
HgaI GACGC 2 cut(s) 636, 1257
HhaI GCGC 3 cut(s) 97, 1624, 2609
Hin1I GRCGYC 3 cut(s) 95, 647, 1249
Hin6I GCGC 3 cut(s) 95, 1622, 2607
HinP1I GCGC 3 cut(s) 95, 1622, 2607
HincII GTYRAC 3 cut(s) 592, 1081, 1435
HindII GTYRAC 3 cut(s) 592, 1081, 1435
HindIII AAGCTT 2 cut(s) 2129, 2300
HpaI GTTAAC 2 cut(s) 592, 1435
HpaII CCGG 9 cut(s) 350, 398, 444, 498, 512, 1894, 2264, 2319, 2525
HphI GGTGA 5 cut(s) 266, 314, 735, 836, 1580
Hpy166II GTNNAC 5 cut(s) 592, 1081, 1357, 1435, 2587
Hpy8I GTNNAC 5 cut(s) 592, 1081, 1357, 1435, 2587
HpyCH4III ACNGT 3 cut(s) 163, 211, 1354
HpyCH4IV ACGT 5 cut(s) 23, 81, 384, 1224, 1539
HpyF10VI GCNNNNNNNGC 9 cut(s) 407, 444, 449, 919, 1543, 1552, 2615, 2662, 2677
HpyF3I CTNAG 9 cut(s) 406, 1120, 1192, 1557, 1776, 1859, 1927, 2657, 2666
HpySE526I ACGT 5 cut(s) 23, 81, 384, 1224, 1539
Hsp92I GRCGYC 3 cut(s) 95, 647, 1249
HspAI GCGC 3 cut(s) 95, 1622, 2607
KasI GGCGCC 1 cut(s) 94
Kpn2I TCCGGA 2 cut(s) 497, 2263
KroI GCCGGC 1 cut(s) 443
KroNI GCCGGC 1 cut(s) 445
Ksp22I TGATCA 2 cut(s) 1129, 2193
KspAI GTTAAC 2 cut(s) 592, 1435
LguI GCTCTTC 1 cut(s) 2646
LmnI GCTCC 9 cut(s) 347, 674, 1102, 1896, 2017, 2086, 2266, 2511, 2644
Lsp1109I GCAGC 6 cut(s) 422, 527, 867, 900, 1546, 2421
LweI GCATC 6 cut(s) 287, 323, 439, 954, 1044, 1440
MaeI CTAG 5 cut(s) 326, 918, 2030, 2495, 2547
MaeII ACGT 5 cut(s) 23, 81, 384, 1224, 1539
MaeIII GTNAC 4 cut(s) 24, 385, 781, 2323
MfeI CAATTG 2 cut(s) 573, 1046
MflI RGATCY 1 cut(s) 2279
MhlI GDGCHC 7 cut(s) 288, 356, 1366, 1406, 2022, 2263, 2516
Mly113I GGCGCC 1 cut(s) 95
MlyI GAGTC 7 cut(s) 169, 182, 227, 468, 510, 789, 1960
MmeI TCCRAC 2 cut(s) 772, 799
MroI TCCGGA 2 cut(s) 497, 2263
MroNI GCCGGC 1 cut(s) 443
MroXI GAANNNNTTC 2 cut(s) 630, 2619
MslI CAYNNNNRTG 5 cut(s) 438, 1233, 1413, 1599, 2147
MspA1I CMGCKG 1 cut(s) 2465
MspCI CTTAAG 1 cut(s) 1268
MspI CCGG 9 cut(s) 350, 398, 444, 498, 512, 1894, 2264, 2319, 2525
MspR9I CCNGG 4 cut(s) 232, 350, 351, 2112
MunI CAATTG 2 cut(s) 573, 1046
MvaI CCWGG 2 cut(s) 232, 2112
MvnI CGCG 1 cut(s) 2448
MwoI GCNNNNNNNGC 9 cut(s) 407, 444, 449, 919, 1543, 1552, 2615, 2662, 2677
NaeI GCCGGC 1 cut(s) 445
NarI GGCGCC 1 cut(s) 95
NciI CCSGG 2 cut(s) 350, 351
NgoMIV GCCGGC 1 cut(s) 443
NlaIV GGNNCC 8 cut(s) 96, 236, 565, 1098, 1898, 1992, 2082, 2296
NmeAIII GCCGAG 1 cut(s) 2635
NmuCI GTSAC 2 cut(s) 385, 2323
NspI RCATGY 2 cut(s) 1232, 1412
PaeI GCATGC 1 cut(s) 1412
PaeR7I CTCGAG 1 cut(s) 419
PagI TCATGA 1 cut(s) 2340
PaqCI CACCTGC 1 cut(s) 2671
PceI AGGCCT 2 cut(s) 230, 1555
PciSI GCTCTTC 1 cut(s) 2646
PdiI GCCGGC 1 cut(s) 445
PdmI GAANNNNTTC 2 cut(s) 630, 2619
PfeI GAWTC 7 cut(s) 494, 626, 1307, 1426, 2050, 2098, 2450
PfoI TCCNGGA 1 cut(s) 2110
PkrI GCNGC 7 cut(s) 266, 412, 517, 857, 915, 1536, 2411
PleI GAGTC 7 cut(s) 169, 182, 226, 467, 509, 789, 1960
PluTI GGCGCC 1 cut(s) 98
PmaCI CACGTG 1 cut(s) 82
PmlI CACGTG 1 cut(s) 82
PpsI GAGTC 7 cut(s) 169, 182, 226, 467, 509, 789, 1960
Ppu21I YACGTR 1 cut(s) 82
PpuMI RGGWCCY 1 cut(s) 2231
PshBI ATTAAT 2 cut(s) 527, 2471
Psp124BI GAGCTC 3 cut(s) 288, 2022, 2263
Psp5II RGGWCCY 1 cut(s) 2231
Psp6I CCWGG 2 cut(s) 230, 2110
PspCI CACGTG 1 cut(s) 82
PspGI CCWGG 2 cut(s) 230, 2110
PspN4I GGNNCC 8 cut(s) 96, 236, 565, 1098, 1898, 1992, 2082, 2296
PspPI GGNCC 5 cut(s) 506, 564, 1368, 2231, 2294
PspPPI RGGWCCY 1 cut(s) 2231
PspXI VCTCGAGB 1 cut(s) 419
PsrI GAACNNNNNNTAC 2 cut(s) 365, 397
PstI CTGCAG 2 cut(s) 701, 1536
PstNI CAGNNNCTG 2 cut(s) 1022, 2686
PsuI RGATCY 1 cut(s) 2279
RsaI GTAC 4 cut(s) 1197, 1530, 1743, 2034
RsaNI GTAC 4 cut(s) 1196, 1529, 1742, 2033
RseI CAYNNNNRTG 5 cut(s) 438, 1233, 1413, 1599, 2147
SacI GAGCTC 3 cut(s) 288, 2022, 2263
SapI GCTCTTC 1 cut(s) 2646
SatI GCNGC 7 cut(s) 265, 411, 516, 856, 914, 1535, 2410
Sau96I GGNCC 5 cut(s) 506, 564, 1368, 2231, 2294
SchI GAGTC 7 cut(s) 169, 182, 227, 468, 510, 789, 1960
ScrFI CCNGG 4 cut(s) 232, 350, 351, 2112
SduI GDGCHC 7 cut(s) 288, 356, 1366, 1406, 2022, 2263, 2516
SfaNI GCATC 6 cut(s) 287, 323, 439, 954, 1044, 1440
SfcI CTRYAG 2 cut(s) 697, 1532
SfoI GGCGCC 1 cut(s) 96
Sfr274I CTCGAG 1 cut(s) 419
SinI GGWCC 3 cut(s) 564, 1368, 2231
SlaI CTCGAG 1 cut(s) 419
SmaI CCCGGG 1 cut(s) 351
SmiMI CAYNNNNRTG 5 cut(s) 438, 1233, 1413, 1599, 2147
SmlI CTYRAG 3 cut(s) 419, 1268, 2275
SmoI CTYRAG 3 cut(s) 419, 1268, 2275
SphI GCATGC 1 cut(s) 1412
SseBI AGGCCT 2 cut(s) 230, 1555
SspDI GGCGCC 1 cut(s) 94
SspMI CTAG 5 cut(s) 326, 918, 2030, 2495, 2547
SstI GAGCTC 3 cut(s) 288, 2022, 2263
StuI AGGCCT 2 cut(s) 230, 1555
StyD4I CCNGG 4 cut(s) 230, 348, 349, 2110
StyI CCWWGG 2 cut(s) 917, 2005
TaaI ACNGT 3 cut(s) 163, 211, 1354
TaiI ACGT 5 cut(s) 26, 84, 387, 1227, 1542
TaqI TCGA 7 cut(s) 295, 371, 420, 1747, 2490, 2568, 2623
TatI WGTACW 3 cut(s) 1195, 1528, 2032
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 7 cut(s) 494, 626, 1307, 1426, 2050, 2098, 2450
TscAI CASTG 5 cut(s) 445, 459, 706, 1357, 2589
TseFI GTSAC 2 cut(s) 385, 2323
TseI GCWGC 6 cut(s) 410, 515, 855, 913, 1534, 2409
Tsp45I GTSAC 2 cut(s) 385, 2323
TspGWI ACGGA 5 cut(s) 43, 122, 572, 1381, 2631
TspMI CCCGGG 1 cut(s) 349
TspRI CASTG 5 cut(s) 445, 459, 706, 1357, 2589
Vha464I CTTAAG 1 cut(s) 1268
VpaK11BI GGWCC 3 cut(s) 564, 1368, 2231
VspI ATTAAT 2 cut(s) 527, 2471
XagI CCTNNNNNAGG 1 cut(s) 2033
XapI RAATTY 7 cut(s) 88, 706, 877, 892, 1025, 1292, 1513
XbaI TCTAGA 1 cut(s) 2494
XceI RCATGY 2 cut(s) 1232, 1412
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XhoI CTCGAG 1 cut(s) 419
XmaI CCCGGG 1 cut(s) 349
XmaJI CCTAGG 1 cut(s) 917
XmnI GAANNNNTTC 2 cut(s) 630, 2619
XspI CTAG 5 cut(s) 326, 918, 2030, 2495, 2547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.