Rorug07G0089300

serine threonine-protein kinase cdc7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002088
Physical Location & Seq
Reverse (-)
7021707 .. 7023405
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0089300.1

Sequence Viewer

Length: 1587 bp
ATGATCTCCAATCTCATCACCCTAATCAAACACTGCAAAACCCCCAAGCAACTCCTCCAACTCCATGCTCATACCATCACCAATGCCACTCTCACTATACCCCATCACAAAATCCTACCCAACCTCCTCCACAGCTTTATCTCCCTAATCAAACCCTCTTCCTCCGCACCTTCTACCACCACCTCACTCAGCTATGCCGTCTCTCTCTTCAATCTCATCCCAAACCCATCTACTTTCTGCTTCAACAACATCATCAGAGCTCACACCCTCCTCTCCTCTCCCCTCCCTGCCCTCCATCTCTTCGCTTCCATGCGCCTTTTTTCGCTCCCCCCAGACTTCCACACCTTCCCTTTCGCCCTCAAGGCCTGCGCCCAGCTTGGCTCTTCTTCATTTTCAATCACCCAGACCCTTCATTCTCAAGCCCTCAAGTTCGGTTTCGCTTCCCACTCGTTTGTCACCAACACCCTCATTCATGTCTACTCAGCTTGTCAGCATCTGGACCATGCACGCAAGGTGTTCGACGAAAGTTCCCACAGGGATTTGGTCTCGTATAATGCGATGCTGGACGCATTTGTTAAGGGAGGTGAGATTTGTAGAGCGCGGCAAGTGTTTGATGAGATGGCAGACCGGGACTCTGTTTCGTGGGGGACTATTTTAGCTGGGTATGCACAGACGAATCGGTGCGAGGAAGCGATTGAGCTGTTTGGTGAGATGATTGAGTCGAACATTAGGCCTGATGATATCTGCTTGGTCTCTGCTCTCTCTGCTTGTTCTCAGCTGGGTGATTTAGAACGAGGGAGAAGTATCCATGACTACATCAAACGAAACAGGATTAGACTGAGTTCATTCTTTTTGACAGCATTGGTGGACTTGTATGCGAAATGCGGGTGCATCGAGACTGCCATTAAGATTTTCGAGTCGAGCTGTGATAAGAATGTGTTCACTTGGAATGCCATGCTTGTTGGGCTTGCAATGCACGGCCACGCCCATCTCTCACTGGAGTACTTCTCCAGAATGATAGAGGCTGGAATTAAACCTGATGGGGTTAGCTTATTAGGGGTTTTAGTGGGTTGCAGCCATGGAGGTAGAGTAGATGAAGCCCGAAAGCTTTTCGATGAAATGGAATTAGTTTATGGGGTCCCCAGAGAGCTCAAGCATTATGGGTGCATGGCTGATCTGCTTGGTAGAGCTGGGTTGATCAAGGAAGCAATGGAGATGATAGAGAAGATGCCAATGGGGGGCGATGTGTTTGTGTGGGGTGGTTTACTTGGAGGTTGCAAAATACATGGTGATGTTGAGGGTGCCAAGAAAGCAGCAGAGCATGTGATGGAGTTGGACCCTGAAGATGGTGGTGTGTATTCAATCTTGGCTAGCGTTTATGCCAATGCAGAGAAATGGGATGATGTGGTAAAAACCAGAAAGTCCATAAGTTCTAACAAGAGGGTCAAGAAGAACTCTGGTTGTAGTTTGATTAGGTTGAATGGAGTCACCCATGAGTTTCTTGCAGGGGATAGCTTCCATCCTCGAACAGAAGCAATCTATTTGGTACTGGATGGACTTTGGAAACATCAATTTGAATCATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006950 GO:0006974 GO:0007049 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0008360 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010389 GO:0010556 GO:0010557 GO:0010564 GO:0010571 GO:0010604 GO:0010948 GO:0010971 GO:0015630 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0033262 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045171 GO:0045740 GO:0045786 GO:0045787 GO:0045931 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070316 GO:0070317 GO:0071704 GO:0072686 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0098657 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1903047 GO:2000105 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 map04110 map04111 map04113
Pfam Domains
Protein Families
Pkinase

Protein Analysis

528

Amino Acids

58.38

Weight (kDa)

6.81

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 211 - 257 3.7e-08 PPR repeat family
PPR PF01535 213 - 243 8.3e-07 PPR repeat
E_motif PF20431 429 - 491 3e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1301
AccI GTMKAC 1 cut(s) 477
AccII CGCG 1 cut(s) 601
AciI CCGC 3 cut(s) 165, 601, 885
AcoI YGGCCR 1 cut(s) 979
AcuI CTGAAG 1 cut(s) 1362
AfaI GTAC 2 cut(s) 1004, 1548
AfiI CCNNNNNNNGG 2 cut(s) 1238, 1346
AgsI TTSAA 6 cut(s) 211, 244, 396, 1362, 1480, 1577
Alw21I GWGCWC 2 cut(s) 262, 1152
Alw26I GTCTC 4 cut(s) 205, 550, 757, 890
AlwNI CAGNNNCTG 1 cut(s) 496
AoxI GGCC 3 cut(s) 363, 731, 979
ApeKI GCWGC 2 cut(s) 1074, 1313
Asp700I GAANNNNTTC 1 cut(s) 938
AspLEI GCGC 3 cut(s) 315, 371, 601
AspS9I GGNCC 3 cut(s) 499, 1138, 1336
AsuC2I CCSGG 1 cut(s) 629
AsuHPI GGTGA 9 cut(s) 10, 70, 391, 448, 596, 719, 794, 1301, 1480
AsuNHI GCTAGC 1 cut(s) 1370
AvaII GGWCC 3 cut(s) 499, 1138, 1336
BanI GGYRCC 1 cut(s) 1301
BanII GRGCYC 2 cut(s) 262, 1152
Bbv12I GWGCWC 2 cut(s) 262, 1152
BbvI GCAGC 2 cut(s) 1086, 1325
BceAI ACGGC 2 cut(s) 182, 994
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BciVI GTATCC 1 cut(s) 815
BclI TGATCA 1 cut(s) 1197
BcnI CCSGG 1 cut(s) 629
BcoDI GTCTC 4 cut(s) 205, 550, 757, 890
BfaI CTAG 2 cut(s) 1371, 1585
BfuI GTATCC 1 cut(s) 815
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 3 cut(s) 602, 1075, 1314
BlsI GCNGC 3 cut(s) 603, 1076, 1315
BmcAI AGTACT 1 cut(s) 1004
Bme1390I CCNGG 1 cut(s) 629
Bme18I GGWCC 3 cut(s) 499, 1138, 1336
BmgT120I GGNCC 3 cut(s) 499, 1138, 1336
BmiI GGNNCC 4 cut(s) 1139, 1140, 1303, 1338
BmrFI CCNGG 1 cut(s) 629
BmsI GCATC 4 cut(s) 502, 549, 900, 1218
BmtI GCTAGC 1 cut(s) 1374
BplI GAGNNNNNCTC 2 cut(s) 992, 1024
BpmI CTGGAG 2 cut(s) 994, 1019
BpuEI CTTGAG 4 cut(s) 344, 402, 410, 1136
BpuMI CCSGG 1 cut(s) 629
BsaI GGTCTC 2 cut(s) 550, 757
BsaJI CCNNGG 1 cut(s) 1078
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 1074, 1104
Bsc4I CCNNNNNNNGG 2 cut(s) 1238, 1346
Bse1I ACTGG 2 cut(s) 1002, 1554
Bse3DI GCAATG 2 cut(s) 978, 1215
BseDI CCNNGG 1 cut(s) 1078
BseGI GGATG 4 cut(s) 216, 1405, 1519, 1558
BseLI CCNNNNNNNGG 2 cut(s) 1238, 1346
BseMI GCAATG 2 cut(s) 978, 1215
BseMII CTCAG 4 cut(s) 202, 495, 788, 830
BseNI ACTGG 2 cut(s) 1002, 1554
BseRI GAGGAG 4 cut(s) 44, 116, 260, 265
BseXI GCAGC 2 cut(s) 1086, 1325
BseYI CCCAGC 4 cut(s) 372, 659, 778, 1190
Bsh1236I CGCG 1 cut(s) 601
BshFI GGCC 3 cut(s) 365, 733, 981
BshNI GGYRCC 1 cut(s) 1301
BsiHKAI GWGCWC 2 cut(s) 262, 1152
BsiSI CCGG 1 cut(s) 628
BslFI GGGAC 3 cut(s) 644, 661, 1124
BslI CCNNNNNNNGG 2 cut(s) 1238, 1346
BsmAI GTCTC 4 cut(s) 205, 550, 757, 890
BsmBI CGTCTC 1 cut(s) 205
BsmFI GGGAC 3 cut(s) 644, 661, 1124
BsmI GAATGC 1 cut(s) 955
BsnI GGCC 3 cut(s) 365, 733, 981
Bso31I GGTCTC 2 cut(s) 550, 757
Bsp1286I GDGCHC 2 cut(s) 262, 1152
Bsp143I GATC 3 cut(s) 3, 1174, 1197
Bsp19I CCATGG 1 cut(s) 1078
BspACI CCGC 3 cut(s) 165, 601, 885
BspANI GGCC 3 cut(s) 365, 733, 981
BspCNI CTCAG 4 cut(s) 201, 494, 787, 831
BspFNI CGCG 1 cut(s) 601
BspLI GGNNCC 4 cut(s) 1139, 1140, 1303, 1338
BspOI GCTAGC 1 cut(s) 1374
BspQI GCTCTTC 1 cut(s) 388
BspT107I GGYRCC 1 cut(s) 1301
BspTNI GGTCTC 2 cut(s) 550, 757
BsrDI GCAATG 2 cut(s) 978, 1215
BsrI ACTGG 2 cut(s) 1002, 1554
BssECI CCNNGG 1 cut(s) 1078
BssMI GATC 3 cut(s) 3, 1174, 1197
BssT1I CCWWGG 1 cut(s) 1078
Bst6I CTCTTC 4 cut(s) 163, 212, 305, 388
BstC8I GCNNGC 4 cut(s) 367, 508, 969, 1372
BstDEI CTNAG 4 cut(s) 188, 481, 774, 839
BstDSI CCRYGG 1 cut(s) 1078
BstF5I GGATG 4 cut(s) 216, 1405, 1519, 1558
BstFNI CGCG 1 cut(s) 601
BstHHI GCGC 3 cut(s) 315, 371, 601
BstKTI GATC 3 cut(s) 6, 1177, 1200
BstMAI GTCTC 4 cut(s) 205, 550, 757, 890
BstMBI GATC 3 cut(s) 3, 1174, 1197
BstMWI GCNNNNNNNGC 6 cut(s) 362, 665, 764, 964, 973, 1310
BstNSI RCATGY 1 cut(s) 1325
BstSCI CCNGG 1 cut(s) 627
BstUI CGCG 1 cut(s) 601
BstV1I GCAGC 2 cut(s) 1086, 1325
BsuI GTATCC 1 cut(s) 815
BsuRI GGCC 3 cut(s) 365, 733, 981
BtgI CCRYGG 1 cut(s) 1078
BtgZI GCGATG 2 cut(s) 572, 1257
BtsCI GGATG 4 cut(s) 216, 1405, 1519, 1558
BtsI GCAGTG 1 cut(s) 31
BtsIMutI CAGTG 2 cut(s) 31, 995
Cac8I GCNNGC 4 cut(s) 367, 508, 969, 1372
CaiI CAGNNNCTG 1 cut(s) 496
CfoI GCGC 3 cut(s) 315, 371, 601
Cfr13I GGNCC 3 cut(s) 499, 1138, 1336
CseI GACGC 1 cut(s) 575
Csp6I GTAC 2 cut(s) 1003, 1547
CviQI GTAC 2 cut(s) 1003, 1547
DdeI CTNAG 4 cut(s) 188, 481, 774, 839
DpnI GATC 3 cut(s) 5, 1176, 1199
DpnII GATC 3 cut(s) 3, 1174, 1197
EaeI YGGCCR 1 cut(s) 979
Eam1104I CTCTTC 4 cut(s) 163, 212, 305, 388
EarI CTCTTC 4 cut(s) 163, 212, 305, 388
Ecl136II GAGCTC 2 cut(s) 260, 1150
Eco130I CCWWGG 1 cut(s) 1078
Eco147I AGGCCT 2 cut(s) 365, 733
Eco24I GRGCYC 2 cut(s) 262, 1152
Eco31I GGTCTC 2 cut(s) 550, 757
Eco32I GATATC 1 cut(s) 742
Eco47I GGWCC 3 cut(s) 499, 1138, 1336
Eco53kI GAGCTC 2 cut(s) 260, 1150
Eco57I CTGAAG 1 cut(s) 1362
EcoICRI GAGCTC 2 cut(s) 260, 1150
EcoO109I RGGNCCY 1 cut(s) 1138
EcoRV GATATC 1 cut(s) 742
EcoT14I CCWWGG 1 cut(s) 1078
EcoT38I GRGCYC 2 cut(s) 262, 1152
ErhI CCWWGG 1 cut(s) 1078
Esp3I CGTCTC 1 cut(s) 205
FaqI GGGAC 3 cut(s) 644, 661, 1124
FauI CCCGC 1 cut(s) 878
FbaI TGATCA 1 cut(s) 1197
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 3 cut(s) 602, 1075, 1314
FokI GGATG 4 cut(s) 203, 1412, 1506, 1565
FriOI GRGCYC 2 cut(s) 262, 1152
Fsp4HI GCNGC 3 cut(s) 602, 1075, 1314
FspBI CTAG 2 cut(s) 1371, 1585
GlaI GCGC 3 cut(s) 314, 370, 600
GluI GCNGC 3 cut(s) 602, 1075, 1314
GsaI CCCAGC 4 cut(s) 376, 663, 782, 1194
GsuI CTGGAG 2 cut(s) 994, 1019
HaeIII GGCC 3 cut(s) 365, 733, 981
HapII CCGG 1 cut(s) 628
HgaI GACGC 1 cut(s) 575
HhaI GCGC 3 cut(s) 315, 371, 601
Hin6I GCGC 3 cut(s) 313, 369, 599
HinP1I GCGC 3 cut(s) 313, 369, 599
HindIII AAGCTT 1 cut(s) 1106
HinfI GANTC 6 cut(s) 632, 676, 719, 917, 1485, 1577
HpaII CCGG 1 cut(s) 628
HphI GGTGA 9 cut(s) 10, 70, 391, 448, 596, 719, 794, 1301, 1480
Hpy166II GTNNAC 4 cut(s) 478, 868, 942, 1265
Hpy188I TCNGA 1 cut(s) 257
Hpy188III TCNNGA 4 cut(s) 497, 895, 1011, 1447
Hpy8I GTNNAC 4 cut(s) 478, 868, 942, 1265
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 3 cut(s) 180, 355, 419
HpyF10VI GCNNNNNNNGC 6 cut(s) 362, 665, 764, 964, 973, 1310
HpyF3I CTNAG 4 cut(s) 188, 481, 774, 839
HspAI GCGC 3 cut(s) 313, 369, 599
KflI GGGWCCC 1 cut(s) 1138
Ksp22I TGATCA 1 cut(s) 1197
Kzo9I GATC 3 cut(s) 3, 1174, 1197
LguI GCTCTTC 1 cut(s) 388
LmnI GCTCC 1 cut(s) 330
Lsp1109I GCAGC 2 cut(s) 1086, 1325
LweI GCATC 4 cut(s) 502, 549, 900, 1218
MaeI CTAG 2 cut(s) 1371, 1585
MaeIII GTNAC 2 cut(s) 454, 1486
MalI GATC 3 cut(s) 5, 1176, 1199
MboI GATC 3 cut(s) 3, 1174, 1197
MboII GAAGA 8 cut(s) 150, 199, 292, 375, 378, 1237, 1355, 1462
MhlI GDGCHC 2 cut(s) 262, 1152
MluCI AATT 3 cut(s) 1029, 1124, 1571
MlyI GAGTC 4 cut(s) 626, 728, 926, 1494
MmeI TCCRAC 2 cut(s) 82, 1314
MroXI GAANNNNTTC 1 cut(s) 938
MseI TTAA 3 cut(s) 576, 906, 1032
MslI CAYNNNNRTG 1 cut(s) 1290
MspA1I CMGCKG 1 cut(s) 778
MspI CCGG 1 cut(s) 628
MspR9I CCNGG 1 cut(s) 629
Mva1269I GAATGC 1 cut(s) 955
MvnI CGCG 1 cut(s) 601
MwoI GCNNNNNNNGC 6 cut(s) 362, 665, 764, 964, 973, 1310
NciI CCSGG 1 cut(s) 629
NcoI CCATGG 1 cut(s) 1078
NdeII GATC 3 cut(s) 3, 1174, 1197
NheI GCTAGC 1 cut(s) 1370
NlaIV GGNNCC 4 cut(s) 1139, 1140, 1303, 1338
NmuCI GTSAC 2 cut(s) 454, 1486
NspI RCATGY 1 cut(s) 1325
PceI AGGCCT 2 cut(s) 365, 733
PciSI GCTCTTC 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 554
PctI GAATGC 1 cut(s) 955
PdmI GAANNNNTTC 1 cut(s) 938
PfeI GAWTC 2 cut(s) 676, 1577
PkrI GCNGC 3 cut(s) 603, 1076, 1315
PleI GAGTC 4 cut(s) 626, 727, 925, 1493
PpsI GAGTC 4 cut(s) 626, 727, 925, 1493
PpuMI RGGWCCY 1 cut(s) 1138
Psp124BI GAGCTC 2 cut(s) 262, 1152
Psp5II RGGWCCY 1 cut(s) 1138
PspFI CCCAGC 4 cut(s) 372, 659, 778, 1190
PspN4I GGNNCC 4 cut(s) 1139, 1140, 1303, 1338
PspPI GGNCC 3 cut(s) 499, 1138, 1336
PspPPI RGGWCCY 1 cut(s) 1138
PstNI CAGNNNCTG 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 778
RsaI GTAC 2 cut(s) 1004, 1548
RsaNI GTAC 2 cut(s) 1003, 1547
RseI CAYNNNNRTG 1 cut(s) 1290
SacI GAGCTC 2 cut(s) 262, 1152
SapI GCTCTTC 1 cut(s) 388
SaqAI TTAA 3 cut(s) 576, 906, 1032
SatI GCNGC 3 cut(s) 602, 1075, 1314
Sau3AI GATC 3 cut(s) 3, 1174, 1197
Sau96I GGNCC 3 cut(s) 499, 1138, 1336
ScaI AGTACT 1 cut(s) 1004
SchI GAGTC 4 cut(s) 626, 728, 926, 1494
ScrFI CCNGG 1 cut(s) 629
SduI GDGCHC 2 cut(s) 262, 1152
SfaNI GCATC 4 cut(s) 502, 549, 900, 1218
SinI GGWCC 3 cut(s) 499, 1138, 1336
SmiMI CAYNNNNRTG 1 cut(s) 1290
SmlI CTYRAG 4 cut(s) 359, 417, 425, 1151
SmoI CTYRAG 4 cut(s) 359, 417, 425, 1151
Sse9I AATT 3 cut(s) 1029, 1124, 1571
SseBI AGGCCT 2 cut(s) 365, 733
SsiI CCGC 3 cut(s) 165, 601, 885
SspMI CTAG 2 cut(s) 1371, 1585
SstI GAGCTC 2 cut(s) 262, 1152
StuI AGGCCT 2 cut(s) 365, 733
StyD4I CCNGG 1 cut(s) 627
StyI CCWWGG 1 cut(s) 1078
TaqI TCGA 7 cut(s) 519, 722, 894, 915, 920, 1113, 1525
TasI AATT 3 cut(s) 1029, 1124, 1571
TatI WGTACW 1 cut(s) 1002
TauI GCSGC 1 cut(s) 604
TfiI GAWTC 2 cut(s) 676, 1577
Tru1I TTAA 3 cut(s) 576, 906, 1032
Tru9I TTAA 3 cut(s) 576, 906, 1032
TscAI CASTG 2 cut(s) 38, 1002
TseFI GTSAC 2 cut(s) 454, 1486
TseI GCWGC 2 cut(s) 1074, 1313
Tsp45I GTSAC 2 cut(s) 454, 1486
TspDTI ATGAA 6 cut(s) 378, 401, 461, 834, 1110, 1131
TspRI CASTG 2 cut(s) 38, 1002
VpaK11BI GGWCC 3 cut(s) 499, 1138, 1336
XceI RCATGY 1 cut(s) 1325
XmiI GTMKAC 1 cut(s) 477
XmnI GAANNNNTTC 1 cut(s) 938
XspI CTAG 2 cut(s) 1371, 1585
ZrmI AGTACT 1 cut(s) 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.