Rh7AG219800

serine threonine-protein kinase cdc7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0002088
Physical Location & Seq
Forward (+)
20450352 .. 20456732
6381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG219800.1

Sequence Viewer

Length: 3597 bp
ATGGCAACTAACCCACTCGACTCAGCCCTCACCGCCTCAACCATCTCCGACTCCGACAAGGCTTGGCACCTCCTCGCCCTCCTCTTATCTCTCGGCCGCCGCACCCCGCCCTCTGAGCTCGCCGCCCGATGCACGGCGTTCCCAGCAACTACTCATTCGGTCCGACGCCTTTGCTCCGTCGCCAATTCGCCGCTCTCTTTGTTCGACGATCTATACGTCACGCCGTCTCTACTCGCGGTAACCGCTCTGGCGGAGTCCGTTTCATGTTCCGGCGGTGCACTGAGAACCTACTCAGGGAAGAGGAAGCGCGTCGTTTTGGATTGCGGATTTGTGCCCTCGGCCAAACGGAGATTGATTGTGGATTTTGAAAATGTATATGAAGAGAAAGGAGCACGGAAATCAGTCGTAATTTCTCCTCCAAAGATACATTTTCATATGACTGACTACCTCAGCAGAAAAATAAATATGCTACCAAGCGATGTGGCTGTGGGGTTGAATAAACCGATGTTCAGTTCTCCATTATTTATTGAATATAGTGCTGAGCCATCCAGTGGTGGAGTGAAGAACATTGATAATGTAGAAGATCAAATGGAGACCGATCTAACTCTGCAAAGGAACATTAACAAAACAGTTATGGCCTGTGTAGATGATTTAGAGCCAACTGTTCCAACAGTGTCAGAAGATTTAGTACTTTATGAAGAAGCTAACATGCACGAGATAGATAGCTTGGCCAAATCTTACCTAGAGAAAAGTAAAGAAAGCATTTTGTCACCTGTGCTGGATGAGAATGTTAGCGGTGGTTGTAAAGTTGCAAATTCTACAATCCTTGAAGATGAAAGAATTGTGGCAGATTCGGAGGAAGAATTGTTAGTAGATCCAGGCAACCGCAATGGTGAAAGTGAAAACATGTCCCACCCACAAACTGCTCTCTCTGATAAGCTGCCTGGAATCGACTCCAGAAGATTAGAAGAGGAGGACTATGAAGATACAATACCTTTGAGAAAAAGGCTTATAAGAATACTCGAGACCCGAGTCAAACTTCCAGTTACTGATTTCTCTGTTGCTGAAAAGCAATTGTTGCAGCCATTTGAAAAGGTGTCCAAGGATTTAGTACTCTATGAAAGAACTAACATGCATGAGAAAACCAAAGAAAGCATAATCTCACCAGCTTTGGATGAGAGTGTTAGCGGTAGTCGTAAAGTTACAAACGCTACAATTCTTGAAGATGTAAGAACTGTGGGAAATCATGAGGAAGAACTGTTCATAGATCCTGGCAACCCTAATGGAGAGACTGAGAATCTATCACAGCCATTTGCTGCTGTTTCTGATAAGCAGCCTGGAACCGGCTCCAGAAGCTTAAAAGAGCAGGATTTTGAAGATACGATACCATTGCATAAAGAGCGACATGAGAACCAAACCATTTGTGTCGCCACTGATCTCTCCTCTCCTAAGGTGTCCAATGATTTAGTACTCTATGAAGGAGCTGACATGCATGAGATAGATATCCAGGGAAACTCTTACCAAGAGAAAATCAAAGAAAGCATAATGCCAGCTTTGGATGAGAGTGTTAGTGGTAACTGTAAAGGTACAAATGCTACAATGCTTGAAGATAAACGAATTGTGGGAGATGCAGAGGAAGGATTGTTAGTAAATCCTGGCAACCCTATTGGAGAAACAGAAATTCTATACCAGCCATATACTAATGTTTCTGATAAGCTGGCTGGAATTGACTTCAGAAGATTGAATGAGGATGATTATGAAGATACAACACCTGTGAATAAAAGTCTTATGACAAGAAATGAGACCCAAACCACCTGTCCTGCTACTGAATTCTATGTTCCTCAAAAGCAGTTGTTGAAGTCCTCTGCAACAATGAAGGCCATGAACAAGGATAAAATATCTTCAAGATTGCAATCTCTATATAGTTGTAGTCTAGAGCAAGATCAGACTGTTGCCACTCCCAAAGTGAACCTCCAACATAAAATGGATCAGAAGACAAATAGCATGGTACACAAATCAAAGCAGAATTGTGGTGACATGCATATTAATGAAAGAAAGAGACGATACAAATCTATTTCCTCTAAGGATCAGCCAGAGAAAAAAGAACTTCCAAATTTTGATTCTTATATGGTAGAAGAAGAAGAAGGTTCAGGTGGTTATGGCACTGTTTACAGGGCGAGGAGTAAGAATGACAGGACAAGGGTTGCCATTAAGTGTCCACATGCTAATGCTCATAAGAGCCATGTTAACAATGAGTTGAGAATGCTGGAAAGGTTTGGGGGGAAGAACTTTGTGATCAAATATGAAGGCTGTATCAAGAATGGAAATTCAAGTTGCTTTGTATTACAGCATGTTGAGCATGATAGGCCTGAGGTTTTAAAGAAAGAGATAGATGTTTGTGAGCTCCGGTGGTATGGATATTGCTTGTTTAGAGCACTTGCAAGCCTTCATAAGCAGGGAATCATACATAGAGATGTTAAACCCGGCAATTTCCTTTTCTCTCGTAAGGCCAACAAGGGTTACCTTATTGATTTCAATCTATCCATGGACTTGCATCAGAAGTATGGAAATAATAGTAAGTCAAGAGGGGGATTTGATGTAAGCAGCAATGATGAGAAGCTTATGACTGCAAAAAATTTACCTCCAATCACACTTAGGAAGTTGCCAAGTGGTAAATCTTTACTAACAGCCAATGGAGAGACCTCAAAAGGTTTGAAATCCACTTTTGATATGAAGGATCTAAAAAAGATGGCTTTCAGCCAAATGAAGGCCAATAATGATTCGGGTAGTAGGAAGGTAATCAAAAGTCAGGGTGCAGATGGCTCAGGCATAACGTCAGTAAAGGATGTTACAAGTACCAGAACTCCTTCGGCAGAAAGGATGAGAGAACCTTTGCCATGCCAAGGAAGGAAAGAACTGATCAGCCTAGTACAGGAAGCAAGGCAGAGTCCAAATCATGAGGCATCAAGTATTTCAGCTCTTATGAGAAAGAGGGTTCCTGCCCCTCCCGTAAATGTTGATGGCAAGCTTTTCCATGTCACTCCGATGCCTATGCACTCAACTACCAGTGGTATTGGTGGTCCATGCTTGATAAGAAGCAGAGGTCTTGGAAAACATAAAGAAGGCCCTTGTGCTGGAACAAAAGGCTTCAGGGCTCCAGAGGTTCTCTTCAGATCTCCACATCAAGGCCCCAAAATTGATATCTGGTCTGCCGGAGTCACGCTACTCTACTTGTTGATTGGCAGAACACCCTTCCTTGGTGAACCTGAACTGAACATCAAGGATATAGCAAAGCTGAGAGGCAGTGAAGATTTATGGGAAGTTGCTAAGCTACACGACCGTGAATCCTCATTTCCAGTGGAGCTCTATAGTACAGATTGTTTGCCCTCTATTAAACTGAAGGAGTGGTGTAGAATGAACACAAAGAGACCGGACTTCTTTGAGCAAATCCCAAGACCCCTTTTCGATCTTGTGGATAAATGCTTGATGGTGAACCCTAGGGTTAGATTGAGTGCAGAGGAAGCGCTTAAACATGAGTTTTTCGCTCCATGCCATGAAGAACTGAGAAAGCACAGGCTTCAAAGGCAGGTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006950 GO:0006974 GO:0007049 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0008360 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010389 GO:0010556 GO:0010557 GO:0010564 GO:0010571 GO:0010604 GO:0010948 GO:0010971 GO:0015630 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0033262 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045171 GO:0045740 GO:0045786 GO:0045787 GO:0045931 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070316 GO:0070317 GO:0071704 GO:0072686 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0098657 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1903047 GO:2000105 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 map04110 map04111 map04113
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1198

Amino Acids

133.92

Weight (kDa)

6.63

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 710 - 855 6.3e-23 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 713 - 858 1.6e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 1045 - 1181 4e-16 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1013
Acc36I ACCTGC 1 cut(s) 3577
AccB1I GGYRCC 1 cut(s) 66
AccB7I CCANNNNNTGG 1 cut(s) 551
AccBSI CCGCTC 2 cut(s) 193, 245
AccII CGCG 2 cut(s) 236, 309
AclWI GGATC 5 cut(s) 869, 1262, 1995, 2094, 2745
AcoI YGGCCR 3 cut(s) 94, 339, 729
AcsI RAATTY 6 cut(s) 814, 1680, 1829, 2113, 2326, 2635
AcuI CTGAAG 4 cut(s) 1717, 3133, 3154, 3419
AcyI GRCGYC 1 cut(s) 166
AfaI GTAC 8 cut(s) 690, 1113, 1470, 1588, 2010, 2857, 2931, 3373
AfeI AGCGCT 1 cut(s) 3525
AflIII ACRYGT 1 cut(s) 906
AjnI CCWGG 6 cut(s) 877, 943, 1270, 1336, 1506, 1654
AjuI GAANNNNNNNTTGG 2 cut(s) 2104, 2136
AleI CACNNNNGTG 1 cut(s) 3339
AloI GAACNNNNNNTCC 2 cut(s) 1244, 1276
Alw21I GWGCWC 6 cut(s) 120, 280, 394, 2406, 2437, 3366
Alw26I GTCTC 8 cut(s) 231, 587, 1019, 1283, 1796, 2053, 2693, 3421
Alw44I GTGCAC 1 cut(s) 276
AlwI GGATC 5 cut(s) 869, 1262, 1995, 2094, 2745
AlwNI CAGNNNCTG 1 cut(s) 1049
Ama87I CYCGRG 2 cut(s) 1022, 1029
Aor51HI AGCGCT 1 cut(s) 3525
ApaLI GTGCAC 1 cut(s) 276
ApeKI GCWGC 5 cut(s) 940, 1081, 1316, 1333, 2604
ApoI RAATTY 6 cut(s) 814, 1680, 1829, 2113, 2326, 2635
ArsI GACNNNNNNTTYG 2 cut(s) 1801, 1833
AseI ATTAAT 1 cut(s) 2046
Asp700I GAANNNNTTC 1 cut(s) 2866
AspA2I CCTAGG 1 cut(s) 3497
AspLEI GCGC 2 cut(s) 309, 3526
AspS9I GGNCC 4 cut(s) 160, 3080, 3125, 3188
AsuC2I CCSGG 1 cut(s) 2484
AsuHPI GGTGA 7 cut(s) 22, 762, 905, 1155, 2045, 3272, 3502
AvaI CYCGRG 2 cut(s) 1022, 1029
AvaII GGWCC 2 cut(s) 160, 3080
AvrII CCTAGG 1 cut(s) 3497
AxyI CCTNAGG 2 cut(s) 1449, 2370
BaeGI GKGCMC 2 cut(s) 280, 336
BalI TGGCCA 1 cut(s) 731
BanI GGYRCC 1 cut(s) 66
BanII GRGCYC 4 cut(s) 120, 2406, 3157, 3366
BarI GAAGNNNNNNTAC 6 cut(s) 672, 704, 975, 1007, 1368, 1400
BauI CACGAG 1 cut(s) 713
BbsI GAAGAC 1 cut(s) 2000
Bbv12I GWGCWC 6 cut(s) 120, 280, 394, 2406, 2437, 3366
BbvCI CCTCAGC 1 cut(s) 449
BbvI GCAGC 5 cut(s) 927, 1093, 1303, 1345, 2616
BccI CCATC 6 cut(s) 50, 553, 2743, 2813, 3014, 3481
BceAI ACGGC 2 cut(s) 150, 208
BcgI CGANNNNNNTGC 6 cut(s) 153, 187, 1372, 1406, 3034, 3068
BciT130I CCWGG 6 cut(s) 879, 945, 1272, 1338, 1508, 1656
BclI TGATCA 2 cut(s) 2295, 2919
BcnI CCSGG 1 cut(s) 2484
BcoDI GTCTC 8 cut(s) 231, 587, 1019, 1283, 1796, 2053, 2693, 3421
BfaI CTAG 4 cut(s) 743, 1934, 2927, 3498
BfmI CTRYAG 1 cut(s) 3367
BfoI RGCGCY 1 cut(s) 3527
BfuAI ACCTGC 1 cut(s) 3577
BglII AGATCT 1 cut(s) 3173
BisI GCNGC 9 cut(s) 97, 100, 123, 191, 941, 1082, 1317, 1334, 2605
BlnI CCTAGG 1 cut(s) 3497
BlpI GCTNAGC 2 cut(s) 540, 3327
BlsI GCNGC 9 cut(s) 98, 101, 124, 192, 942, 1083, 1318, 1335, 2606
BmcAI AGTACT 3 cut(s) 690, 1113, 1470
Bme1390I CCNGG 7 cut(s) 879, 945, 1272, 1338, 1508, 1656, 2484
Bme18I GGWCC 2 cut(s) 160, 3080
BmeT110I CYCGRG 2 cut(s) 1022, 1029
BmgT120I GGNCC 4 cut(s) 160, 3080, 3125, 3188
BmiI GGNNCC 6 cut(s) 68, 1342, 1348, 2997, 3156, 3190
BmrFI CCNGG 7 cut(s) 879, 945, 1272, 1338, 1508, 1656, 2484
BmsI GCATC 5 cut(s) 119, 1618, 2563, 2972, 3036
BoxI GACNNNNGTC 1 cut(s) 1031
BpiI GAAGAC 1 cut(s) 2000
BpmI CTGGAG 3 cut(s) 940, 1333, 3141
Bpu10I CCTNAGC 2 cut(s) 449, 2824
Bpu1102I GCTNAGC 2 cut(s) 540, 3327
BpuMI CCSGG 1 cut(s) 2484
BsaBI GATNNNNATC 4 cut(s) 1502, 1912, 2068, 2535
BsaHI GRCGYC 1 cut(s) 166
BsaI GGTCTC 5 cut(s) 587, 1019, 1796, 2693, 3421
BsaJI CCNNGG 7 cut(s) 336, 1101, 1507, 2544, 2902, 3256, 3497
BsaWI WCCGGW 2 cut(s) 2406, 3430
BsaXI ACNNNNNCTCC 2 cut(s) 2848, 2878
Bse118I RCCGGY 1 cut(s) 1343
Bse1I ACTGG 4 cut(s) 549, 1043, 3066, 3356
Bse21I CCTNAGG 2 cut(s) 1449, 2370
Bse3DI GCAATG 3 cut(s) 895, 1388, 2614
Bse8I GATNNNNATC 4 cut(s) 1502, 1912, 2068, 2535
BseBI CCWGG 6 cut(s) 879, 945, 1272, 1338, 1508, 1656
BseDI CCNNGG 7 cut(s) 336, 1101, 1507, 2544, 2902, 3256, 3497
BseGI GGATG 7 cut(s) 545, 787, 1180, 1564, 1756, 2851, 2886
BseJI GATNNNNATC 4 cut(s) 1502, 1912, 2068, 2535
BseMI GCAATG 3 cut(s) 895, 1388, 2614
BseNI ACTGG 4 cut(s) 549, 1043, 3066, 3356
BseRI GAGGAG 6 cut(s) 62, 71, 405, 986, 1432, 2194
BseSI GKGCMC 2 cut(s) 280, 336
BseX3I CGGCCG 1 cut(s) 94
BseXI GCAGC 5 cut(s) 927, 1093, 1303, 1345, 2616
BseYI CCCAGC 1 cut(s) 142
BsgI GTGCAG 2 cut(s) 2835, 3534
Bsh1236I CGCG 2 cut(s) 236, 309
Bsh1285I CGRYCG 2 cut(s) 97, 3340
BshNI GGYRCC 1 cut(s) 66
BsiEI CGRYCG 2 cut(s) 97, 3340
BsiHKAI GWGCWC 6 cut(s) 120, 280, 394, 2406, 2437, 3366
BsiHKCI CYCGRG 2 cut(s) 1022, 1029
BsiSI CCGG 6 cut(s) 270, 1344, 2407, 2484, 3213, 3431
BslFI GGGAC 1 cut(s) 895
BsmAI GTCTC 8 cut(s) 231, 587, 1019, 1283, 1796, 2053, 2693, 3421
BsmBI CGTCTC 2 cut(s) 231, 2053
BsmFI GGGAC 1 cut(s) 895
BsmI GAATGC 1 cut(s) 2268
Bso31I GGTCTC 5 cut(s) 587, 1019, 1796, 2693, 3421
BsoBI CYCGRG 2 cut(s) 1022, 1029
Bsp1286I GDGCHC 8 cut(s) 120, 280, 336, 394, 2406, 2437, 3157, 3366
Bsp1720I GCTNAGC 2 cut(s) 540, 3327
Bsp19I CCATGG 1 cut(s) 2544
BspFNI CGCG 2 cut(s) 236, 309
BspHI TCATGA 2 cut(s) 1246, 2956
BspLI GGNNCC 6 cut(s) 68, 1342, 1348, 2997, 3156, 3190
BspMI ACCTGC 1 cut(s) 3577
BspPI GGATC 5 cut(s) 869, 1262, 1995, 2094, 2745
BspT107I GGYRCC 1 cut(s) 66
BspTNI GGTCTC 5 cut(s) 587, 1019, 1796, 2693, 3421
BsrBI CCGCTC 2 cut(s) 193, 245
BsrDI GCAATG 3 cut(s) 895, 1388, 2614
BsrFI RCCGGY 1 cut(s) 1343
BsrI ACTGG 4 cut(s) 549, 1043, 3066, 3356
BssAI RCCGGY 1 cut(s) 1343
BssECI CCNNGG 7 cut(s) 336, 1101, 1507, 2544, 2902, 3256, 3497
BssNI GRCGYC 1 cut(s) 166
BssSI CACGAG 1 cut(s) 713
BssT1I CCWWGG 5 cut(s) 1101, 2544, 2902, 3256, 3497
Bst2BI CACGAG 1 cut(s) 713
Bst2UI CCWGG 6 cut(s) 879, 945, 1272, 1338, 1508, 1656
Bst4CI ACNGT 9 cut(s) 631, 664, 673, 1237, 1260, 1580, 1951, 2167, 3341
Bst6I CTCTTC 4 cut(s) 293, 375, 963, 3173
BstACI GRCGYC 1 cut(s) 166
BstAPI GCANNNNNTGC 1 cut(s) 1078
BstC8I GCNNGC 5 cut(s) 120, 1551, 1719, 2443, 3026
BstDSI CCRYGG 1 cut(s) 2544
BstEII GGTNACC 2 cut(s) 238, 2519
BstENI CCTNNNNNAGG 1 cut(s) 2930
BstF5I GGATG 7 cut(s) 545, 787, 1180, 1564, 1756, 2851, 2886
BstFNI CGCG 2 cut(s) 236, 309
BstH2I RGCGCY 1 cut(s) 3527
BstHHI GCGC 2 cut(s) 309, 3526
BstMAI GTCTC 8 cut(s) 231, 587, 1019, 1283, 1796, 2053, 2693, 3421
BstMCI CGRYCG 2 cut(s) 97, 3340
BstNI CCWGG 6 cut(s) 879, 945, 1272, 1338, 1508, 1656
BstNSI RCATGY 7 cut(s) 712, 910, 1135, 1492, 2041, 2225, 2354
BstPAI GACNNNNGTC 1 cut(s) 1031
BstPI GGTNACC 2 cut(s) 238, 2519
BstSCI CCNGG 7 cut(s) 877, 943, 1270, 1336, 1506, 1654, 2482
BstSFI CTRYAG 1 cut(s) 3367
BstSLI GKGCMC 2 cut(s) 280, 336
BstUI CGCG 2 cut(s) 236, 309
BstV1I GCAGC 5 cut(s) 927, 1093, 1303, 1345, 2616
BstV2I GAAGAC 1 cut(s) 2000
BstX2I RGATCY 4 cut(s) 874, 1267, 2737, 3173
BstYI RGATCY 4 cut(s) 874, 1267, 2737, 3173
BstZI CGGCCG 1 cut(s) 94
Bsu36I CCTNAGG 2 cut(s) 1449, 2370
BtgI CCRYGG 1 cut(s) 2544
BtgZI GCGATG 1 cut(s) 492
BtsCI GGATG 7 cut(s) 545, 787, 1180, 1564, 1756, 2851, 2886
BtsI GCAGTG 1 cut(s) 3310
BtsIMutI CAGTG 8 cut(s) 278, 556, 678, 1431, 2163, 3073, 3310, 3363
BveI ACCTGC 1 cut(s) 3577
Cac8I GCNNGC 5 cut(s) 120, 1551, 1719, 2443, 3026
CaiI CAGNNNCTG 1 cut(s) 1049
CciI TCATGA 2 cut(s) 1246, 2956
CfoI GCGC 2 cut(s) 309, 3526
Cfr10I RCCGGY 1 cut(s) 1343
Cfr13I GGNCC 4 cut(s) 160, 3080, 3125, 3188
CpoI CGGWCCG 1 cut(s) 160
CseI GACGC 2 cut(s) 174, 298
Csp6I GTAC 8 cut(s) 689, 1112, 1469, 1587, 2009, 2856, 2930, 3372
CspCI CAANNNNNGTGG 2 cut(s) 462, 497
CspI CGGWCCG 1 cut(s) 160
CviQI GTAC 8 cut(s) 689, 1112, 1469, 1587, 2009, 2856, 2930, 3372
DraI TTTAAA 1 cut(s) 2379
EaeI YGGCCR 3 cut(s) 94, 339, 729
EagI CGGCCG 1 cut(s) 94
Eam1104I CTCTTC 4 cut(s) 293, 375, 963, 3173
EarI CTCTTC 4 cut(s) 293, 375, 963, 3173
EciI GGCGGA 1 cut(s) 266
Ecl136II GAGCTC 3 cut(s) 118, 2404, 3364
EclXI CGGCCG 1 cut(s) 94
Eco130I CCWWGG 5 cut(s) 1101, 2544, 2902, 3256, 3497
Eco147I AGGCCT 1 cut(s) 2368
Eco24I GRGCYC 4 cut(s) 120, 2406, 3157, 3366
Eco31I GGTCTC 5 cut(s) 587, 1019, 1796, 2693, 3421
Eco32I GATATC 2 cut(s) 1504, 3202
Eco47I GGWCC 2 cut(s) 160, 3080
Eco47III AGCGCT 1 cut(s) 3525
Eco52I CGGCCG 1 cut(s) 94
Eco53kI GAGCTC 3 cut(s) 118, 2404, 3364
Eco57I CTGAAG 4 cut(s) 1717, 3133, 3154, 3419
Eco81I CCTNAGG 2 cut(s) 1449, 2370
Eco88I CYCGRG 2 cut(s) 1022, 1029
Eco91I GGTNACC 2 cut(s) 238, 2519
EcoICRI GAGCTC 3 cut(s) 118, 2404, 3364
EcoNI CCTNNNNNAGG 1 cut(s) 2930
EcoO109I RGGNCCY 2 cut(s) 3125, 3188
EcoO65I GGTNACC 2 cut(s) 238, 2519
EcoRI GAATTC 1 cut(s) 1829
EcoRII CCWGG 6 cut(s) 877, 943, 1270, 1336, 1506, 1654
EcoRV GATATC 2 cut(s) 1504, 3202
EcoT14I CCWWGG 5 cut(s) 1101, 2544, 2902, 3256, 3497
EcoT22I ATGCAT 3 cut(s) 1137, 1494, 2043
EcoT38I GRGCYC 4 cut(s) 120, 2406, 3157, 3366
ErhI CCWWGG 5 cut(s) 1101, 2544, 2902, 3256, 3497
Esp3I CGTCTC 2 cut(s) 231, 2053
FaqI GGGAC 1 cut(s) 895
FauI CCCGC 1 cut(s) 114
FauNDI CATATG 1 cut(s) 435
FbaI TGATCA 2 cut(s) 2295, 2919
Fnu4HI GCNGC 9 cut(s) 97, 100, 123, 191, 941, 1082, 1317, 1334, 2605
FokI GGATG 7 cut(s) 532, 794, 1187, 1571, 1763, 2858, 2893
FriOI GRGCYC 4 cut(s) 120, 2406, 3157, 3366
Fsp4HI GCNGC 9 cut(s) 97, 100, 123, 191, 941, 1082, 1317, 1334, 2605
FspBI CTAG 4 cut(s) 743, 1934, 2927, 3498
GlaI GCGC 2 cut(s) 308, 3525
GluI GCNGC 9 cut(s) 97, 100, 123, 191, 941, 1082, 1317, 1334, 2605
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 3 cut(s) 940, 1333, 3141
HaeII RGCGCY 1 cut(s) 3527
HapII CCGG 6 cut(s) 270, 1344, 2407, 2484, 3213, 3431
HgaI GACGC 2 cut(s) 174, 298
HhaI GCGC 2 cut(s) 309, 3526
Hin1I GRCGYC 1 cut(s) 166
Hin6I GCGC 2 cut(s) 307, 3524
HinP1I GCGC 2 cut(s) 307, 3524
HincII GTYRAC 1 cut(s) 2248
HindII GTYRAC 1 cut(s) 2248
HindIII AAGCTT 3 cut(s) 1354, 2618, 3026
HpaI GTTAAC 1 cut(s) 2248
HpaII CCGG 6 cut(s) 270, 1344, 2407, 2484, 3213, 3431
HphI GGTGA 7 cut(s) 22, 762, 905, 1155, 2045, 3272, 3502
Hpy166II GTNNAC 8 cut(s) 278, 1969, 2011, 2170, 2219, 2248, 3263, 3493
Hpy8I GTNNAC 8 cut(s) 278, 1969, 2011, 2170, 2219, 2248, 3263, 3493
Hpy99I CGWCG 4 cut(s) 168, 182, 209, 314
HpyCH4III ACNGT 9 cut(s) 631, 664, 673, 1237, 1260, 1580, 1951, 2167, 3341
HpyCH4IV ACGT 2 cut(s) 216, 2834
HpySE526I ACGT 2 cut(s) 216, 2834
Hsp92I GRCGYC 1 cut(s) 166
HspAI GCGC 2 cut(s) 307, 3524
Ksp22I TGATCA 2 cut(s) 2295, 2919
KspAI GTTAAC 1 cut(s) 2248
LmnI GCTCC 8 cut(s) 179, 389, 1352, 1481, 2409, 3160, 3361, 3550
Lsp1109I GCAGC 5 cut(s) 927, 1093, 1303, 1345, 2616
LweI GCATC 5 cut(s) 119, 1618, 2563, 2972, 3036
MaeI CTAG 4 cut(s) 743, 1934, 2927, 3498
MaeII ACGT 2 cut(s) 216, 2834
MbiI CCGCTC 2 cut(s) 193, 245
MfeI CAATTG 1 cut(s) 1073
MflI RGATCY 4 cut(s) 874, 1267, 2737, 3173
MhlI GDGCHC 8 cut(s) 120, 280, 336, 394, 2406, 2437, 3157, 3366
MlsI TGGCCA 1 cut(s) 731
MluNI TGGCCA 1 cut(s) 731
MlyI GAGTC 7 cut(s) 14, 44, 263, 947, 1041, 2956, 3225
MmeI TCCRAC 5 cut(s) 72, 78, 187, 692, 1999
Mox20I TGGCCA 1 cut(s) 731
Mph1103I ATGCAT 3 cut(s) 1137, 1494, 2043
MroXI GAANNNNTTC 1 cut(s) 2866
MscI TGGCCA 1 cut(s) 731
MseI TTAA 9 cut(s) 621, 1358, 2046, 2211, 2247, 2378, 2478, 3393, 3528
MslI CAYNNNNRTG 5 cut(s) 2046, 2226, 2472, 3044, 3339
Msp20I TGGCCA 1 cut(s) 731
MspI CCGG 6 cut(s) 270, 1344, 2407, 2484, 3213, 3431
MspR9I CCNGG 7 cut(s) 879, 945, 1272, 1338, 1508, 1656, 2484
MunI CAATTG 1 cut(s) 1073
Mva1269I GAATGC 1 cut(s) 2268
MvaI CCWGG 6 cut(s) 879, 945, 1272, 1338, 1508, 1656
MvnI CGCG 2 cut(s) 236, 309
NciI CCSGG 1 cut(s) 2484
NcoI CCATGG 1 cut(s) 2544
NdeI CATATG 1 cut(s) 435
NlaIV GGNNCC 6 cut(s) 68, 1342, 1348, 2997, 3156, 3190
NmeAIII GCCGAG 2 cut(s) 72, 317
NmuCI GTSAC 5 cut(s) 217, 768, 2033, 3037, 3217
NsiI ATGCAT 3 cut(s) 1137, 1494, 2043
NspI RCATGY 7 cut(s) 712, 910, 1135, 1492, 2041, 2225, 2354
OliI CACNNNNGTG 1 cut(s) 3339
PaeR7I CTCGAG 1 cut(s) 1022
PagI TCATGA 2 cut(s) 1246, 2956
PceI AGGCCT 1 cut(s) 2368
PciI ACATGT 1 cut(s) 906
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PctI GAATGC 1 cut(s) 2268
PdmI GAANNNNTTC 1 cut(s) 2866
PfeI GAWTC 7 cut(s) 851, 948, 1297, 2120, 2460, 2780, 3344
PflMI CCANNNNNTGG 1 cut(s) 551
PkrI GCNGC 9 cut(s) 98, 101, 124, 192, 942, 1083, 1318, 1335, 2606
PleI GAGTC 7 cut(s) 14, 44, 262, 947, 1040, 2955, 3224
PpsI GAGTC 7 cut(s) 14, 44, 262, 947, 1040, 2955, 3224
PscI ACATGT 1 cut(s) 906
PshAI GACNNNNGTC 1 cut(s) 1031
PshBI ATTAAT 1 cut(s) 2046
PsiI TTATAA 1 cut(s) 1013
Psp124BI GAGCTC 3 cut(s) 120, 2406, 3366
Psp6I CCWGG 6 cut(s) 877, 943, 1270, 1336, 1506, 1654
PspEI GGTNACC 2 cut(s) 238, 2519
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 6 cut(s) 877, 943, 1270, 1336, 1506, 1654
PspN4I GGNNCC 6 cut(s) 68, 1342, 1348, 2997, 3156, 3190
PspPI GGNCC 4 cut(s) 160, 3080, 3125, 3188
PstNI CAGNNNCTG 1 cut(s) 1049
PsuI RGATCY 4 cut(s) 874, 1267, 2737, 3173
RsaI GTAC 8 cut(s) 690, 1113, 1470, 1588, 2010, 2857, 2931, 3373
RsaNI GTAC 8 cut(s) 689, 1112, 1469, 1587, 2009, 2856, 2930, 3372
RseI CAYNNNNRTG 5 cut(s) 2046, 2226, 2472, 3044, 3339
Rsr2I CGGWCCG 1 cut(s) 160
RsrII CGGWCCG 1 cut(s) 160
SacI GAGCTC 3 cut(s) 120, 2406, 3366
SaqAI TTAA 9 cut(s) 621, 1358, 2046, 2211, 2247, 2378, 2478, 3393, 3528
SatI GCNGC 9 cut(s) 97, 100, 123, 191, 941, 1082, 1317, 1334, 2605
Sau96I GGNCC 4 cut(s) 160, 3080, 3125, 3188
ScaI AGTACT 3 cut(s) 690, 1113, 1470
SchI GAGTC 7 cut(s) 14, 44, 263, 947, 1041, 2956, 3225
ScrFI CCNGG 7 cut(s) 879, 945, 1272, 1338, 1508, 1656, 2484
SduI GDGCHC 8 cut(s) 120, 280, 336, 394, 2406, 2437, 3157, 3366
SfaNI GCATC 5 cut(s) 119, 1618, 2563, 2972, 3036
SfcI CTRYAG 1 cut(s) 3367
Sfr274I CTCGAG 1 cut(s) 1022
SinI GGWCC 2 cut(s) 160, 3080
SlaI CTCGAG 1 cut(s) 1022
SmiMI CAYNNNNRTG 5 cut(s) 2046, 2226, 2472, 3044, 3339
SmlI CTYRAG 1 cut(s) 1022
SmoI CTYRAG 1 cut(s) 1022
SseBI AGGCCT 1 cut(s) 2368
SspMI CTAG 4 cut(s) 743, 1934, 2927, 3498
SstI GAGCTC 3 cut(s) 120, 2406, 3366
StuI AGGCCT 1 cut(s) 2368
StyD4I CCNGG 7 cut(s) 877, 943, 1270, 1336, 1506, 1654, 2482
StyI CCWWGG 5 cut(s) 1101, 2544, 2902, 3256, 3497
TaaI ACNGT 9 cut(s) 631, 664, 673, 1237, 1260, 1580, 1951, 2167, 3341
TaiI ACGT 2 cut(s) 219, 2837
TaqI TCGA 5 cut(s) 18, 204, 951, 1023, 3465
TaqII GACCGA 2 cut(s) 148, 611
TatI WGTACW 5 cut(s) 688, 1111, 1468, 2929, 3371
TauI GCSGC 4 cut(s) 99, 102, 125, 193
TfiI GAWTC 7 cut(s) 851, 948, 1297, 2120, 2460, 2780, 3344
Tru1I TTAA 9 cut(s) 621, 1358, 2046, 2211, 2247, 2378, 2478, 3393, 3528
Tru9I TTAA 9 cut(s) 621, 1358, 2046, 2211, 2247, 2378, 2478, 3393, 3528
TscAI CASTG 8 cut(s) 285, 556, 678, 1438, 2170, 3073, 3310, 3363
TseFI GTSAC 5 cut(s) 217, 768, 2033, 3037, 3217
TseI GCWGC 5 cut(s) 940, 1081, 1316, 1333, 2604
Tsp45I GTSAC 5 cut(s) 217, 768, 2033, 3037, 3217
TspGWI ACGGA 4 cut(s) 166, 247, 361, 409
TspRI CASTG 8 cut(s) 285, 556, 678, 1438, 2170, 3073, 3310, 3363
Van91I CCANNNNNTGG 1 cut(s) 551
VneI GTGCAC 1 cut(s) 276
VpaK11BI GGWCC 2 cut(s) 160, 3080
VspI ATTAAT 1 cut(s) 2046
XagI CCTNNNNNAGG 1 cut(s) 2930
XapI RAATTY 6 cut(s) 814, 1680, 1829, 2113, 2326, 2635
XbaI TCTAGA 1 cut(s) 1933
XceI RCATGY 7 cut(s) 712, 910, 1135, 1492, 2041, 2225, 2354
XhoI CTCGAG 1 cut(s) 1022
XmaJI CCTAGG 1 cut(s) 3497
XmnI GAANNNNTTC 1 cut(s) 2866
XspI CTAG 4 cut(s) 743, 1934, 2927, 3498
ZrmI AGTACT 3 cut(s) 690, 1113, 1470
Zsp2I ATGCAT 3 cut(s) 1137, 1494, 2043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.