MD06G1048400.v1.1

serine threonine-protein kinase cdc7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002088
Physical Location & Seq
Reverse (-)
6542544 .. 6548549
6006 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1048400.v1.1.491

Sequence Viewer

Length: 2835 bp
ATGGAAATGGCGACTCGCCTCACCGACTCAGCTCCCATCACGCCAACCATCTCCGAGTCCGAGAAGGCCTGGTACCTTTTCTCACTCCTCTTGCAACTCGGCCGCCCGACTCACCTCTCCGAGCTCGCCTCCCGATGCAAATTGTTCCCCGCATCGCCTCACCTGGTCAGATTCCTTTGCTCCATCCCGCGCTCTCCCCTCTACTTATCGGGCCAGCTCTATGTCACGCCGTCTCTGGCTTCATTAGCAACTTTCCTAGAGTTCTTTTCATATCGCAGTGCCGGGGATGCAGTGAGAGTCTGCTTGGGGAAGCGGAAACGGGCCGTTTGGGATTTCGGATTTGGGCCTCCGGCAGAAAAGAGATTAATTCTGGATTTTGGAAATGTGAATGAAGAGTGTAGATCGCGGAAGGCAGTTGGAAATACTCGTTCGAAGTCACATTCTCATATGGCTGACTACTACTTCTGCAAATACATAAATACGCTACAAAGTTGGATGACACTAACGGTGAACAAATCGATGTTAAGTTCTTCGTTATTTATCGATTACAGAACCGAACAATCAAGTTCAGGACTGAACAATGTTGATCATGTTGAGGAGCAAGAGGAGGGCACCAATATAATTGTTGAAACGAAGGTTAACAGGCCGGTAATGGATTGTGTAGCTGGTTTAGAACCGGTTCTTTCAATCATGGCATCCGATGATTTAGTCCTTTATGAAGGAGCTAACGTACATGAGATGGACCGCAGTGGGTATTTTTTTCTGAAAGAAATGAAAGAGAACAATATATCACCAGTCGTGGATGAGAATGTTAATGGTAGTTGTAAAGCTACAAATGTAGGAATCATTGAAGTTGAAAGAATTGTTGGAGGTGAGGAGGAAGAGTTGTTATTGGCTCCTCGCAGCCCTAATGGAGAGAGAGAAAATTTCTCCCAGCCAAATACTGCTAGTTATGATATGTTTCCTAGACGTAAGTTCAAAAGATTCAGAAAGGCAGATTGTGAAGATATGATGCCTCTGAATAAAAAGCAAACAAGTAGACATGAGACCAAGATCATATGTTCAGCTACTGAATTTTCTGTTTCGCAAAAGAAATTGTTGAAGCCCTCCTCCAAGACGAAGGGAATTGACAAGGATAAAATGGCTCGGAGACCGCAATCACACAACTTAGAGCATCACCAGGCTGTTGCCACTCCCAAAGAGAACCAGCAGTGTGAAACAAATCAAAAGCCAATCAGCATAGTGCAGAAATTAAAACGGAATTGTGATGGCATGCATGTTAATGAAAGAAATGGACGCCATAAATCTGTTTCTCCTAAGGATCAACCGGAGAAAAGGGAATTTCCAATTTTTGAATCTTATGTTGTAGAAGAAGAAGAAGGTTCAGGCGGTTATGGCACTGTTTACAGGGCACGGTTAAAAAATGACGAGAAAAAGGTCGCCATTAAGTGCCCGCATGCTAATGCTCATAAGCATCATGTTAACAATGAGTTAAGGATGCTGGAAAGGTTTGGGGGCAAAAACTTTGTGATAAAATATGAAGGCCATATCAAGAATGAAAGTTCCAGTTGCTTTGTACTGCAGCACGTTGAGCATGATAGGCCTGAGGTTTTAAAGCAAGAGATAGGTCTTTCTCATCTACATTGGTACGGCTATTGCTTGTTTAAGGCATTGGCAACCCTTCATAGACAGGGAGTAGTACATAGAGATGTTAAACCTGGAAACTTCCTTTTCTCTCGAAGGGCCAACAAGGGTTACCTCATAGATTTCAATCTTGCAACGGATTTGCATTTGAAGCCTGGAACAACTCGCAAATTAAAATCTGGAGATGATGTAAATTGCTATGATGAGAAAATTACAAATGCGATATCTGTACCTAGAACCCCACTTCAGAAGTTGTCAATTTCTAAGTCTTCAATAATAGCCAATGGGGAGACCAAAAAAGGTCTCAAATCCACTTTTGATCTTAAGGACTTGAAAAAAAAGGCTTTCAGCCAAATGAAGCCCTACAATTCTTCGGGTAGCTGGAGCGTAATCAAAAGTCAGGGTGCAGATGGCTCAGGTATAACGTCAGTAAAGGATGTGAGTAATATCAGGACGACTTCAGTAGAAAGTCTGATGGAACCTTTGCCATCCCAAGGAAGGAAAGAGCTCATCAACATAGTACAGGAAGCAATGCAGAATCGAAACCATATATCATCAGGTGTTTCGGCTCCTATGAGAAAGAGGATTCCTGCCCCTCCTAGAAATGAGGATGACAAGCTTTTCTGTATCACTCCGATGCCTCTGCACTCAACTGGCAATGGTGTTGGTGGTGCAGCCTTGATCAGAAGCAGAGGGAGTGGAAAGCAGAAAAAAGAAGGTCCTTGTGCTGGAACTAAAGGATACCGAGCTCCGGAGGTTCTCCTGAGATCCCCACATCAAGGCCTCAAGCTTGATATCTGGTCTGCCGGTGTCACTCTACTCTACTTCATGATTGGCAGAACGCCCTTTGGTGGCGATCCCGAACAGAACATAAAGGACATAGCAAAGTTGAGAGGCAGTGAAGATTTATGGGAAGTTGCCAAGCTACACGACCGCGAATTATCATTTCCAGTGGACCTCTATAGTGCAGAGTCTTTGCCATCTCTCAAATTGCAGGATTGGTGTAGAAGGAGCACAAAGAGGCCGGATTTCTTCAACGAAATCCCTAGACCGCTTTTTGATCTGGTGGATAAGTGCTTGACAGTGAACCCGAGGGTTAGGATAAGCGCCGAGGAAGCACTTCGACATGAGTTTTTTGCTCCATGCCATGAAGAGCTGAGAAAGCTGAGGCATCAGAGGCTGGTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006950 GO:0006974 GO:0007049 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0008360 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010389 GO:0010556 GO:0010557 GO:0010564 GO:0010571 GO:0010604 GO:0010948 GO:0010971 GO:0015630 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0033262 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045171 GO:0045740 GO:0045786 GO:0045787 GO:0045931 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070316 GO:0070317 GO:0071704 GO:0072686 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0098657 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1903047 GO:2000105 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 map04110 map04111 map04113
Pfam Domains
Protein Families
Pkinase

Protein Analysis

945

Amino Acids

106.66

Weight (kDa)

9.2

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 454 - 600 8.1e-23 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 458 - 599 5.5e-11 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 790 - 927 2.8e-16 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 72
AccB1I GGYRCC 2 cut(s) 72, 611
AccI GTMKAC 1 cut(s) 1039
AccII CGCG 3 cut(s) 190, 406, 2580
AccIII TCCGGA 1 cut(s) 2395
AclWI GGATC 3 cut(s) 1329, 2406, 2495
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 3 cut(s) 925, 1073, 1340
AcuI CTGAAG 2 cut(s) 1874, 2088
AcyI GRCGYC 1 cut(s) 1297
AfaI GTAC 7 cut(s) 74, 732, 1578, 1649, 1701, 1875, 2166
AfiI CCNNNNNNNGG 6 cut(s) 2138, 2142, 2372, 2395, 2423, 2495
AflII CTTAAG 1 cut(s) 1967
AgeI ACCGGT 1 cut(s) 676
AjnI CCWGG 5 cut(s) 68, 162, 1179, 1717, 1798
AjuI GAANNNNNNNTTGG 2 cut(s) 849, 881
Alw21I GWGCWC 4 cut(s) 126, 2154, 2395, 2660
Alw26I GTCTC 5 cut(s) 237, 1040, 1144, 1928, 1952
AlwI GGATC 3 cut(s) 1329, 2406, 2495
AlwNI CAGNNNCTG 2 cut(s) 1070, 2824
Ama87I CYCGRG 1 cut(s) 2734
Aor13HI TCCGGA 1 cut(s) 2395
ApeKI GCWGC 3 cut(s) 903, 1582, 2318
ApoI RAATTY 3 cut(s) 925, 1073, 1340
AseI ATTAAT 1 cut(s) 365
AsiGI ACCGGT 1 cut(s) 676
Asp700I GAANNNNTTC 2 cut(s) 678, 2763
Asp718I GGTACC 1 cut(s) 72
AspLEI GCGC 2 cut(s) 192, 2753
AspS9I GGNCC 7 cut(s) 211, 321, 344, 742, 1743, 2363, 2599
AsuC2I CCSGG 1 cut(s) 283
AsuHPI GGTGA 7 cut(s) 13, 104, 152, 520, 783, 884, 1169
AsuII TTCGAA 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 2734
AvaII GGWCC 3 cut(s) 742, 2363, 2599
AxyI CCTNAGG 2 cut(s) 1317, 1605
BaeGI GKGCMC 3 cut(s) 614, 1414, 1454
BanI GGYRCC 2 cut(s) 72, 611
BanII GRGCYC 3 cut(s) 126, 2154, 2395
BarI GAAGNNNNNNTAC 2 cut(s) 56, 88
BbsI GAAGAC 1 cut(s) 1905
Bbv12I GWGCWC 4 cut(s) 126, 2154, 2395, 2660
BbvCI CCTCAGC 1 cut(s) 2810
BbvI GCAGC 3 cut(s) 915, 1594, 2330
BccI CCATC 9 cut(s) 44, 56, 191, 733, 1262, 2048, 2113, 2140, 2632
BceAI ACGGC 3 cut(s) 214, 308, 1666
BcgI CGANNNNNNTGC 2 cut(s) 2269, 2303
BciT130I CCWGG 5 cut(s) 70, 164, 1181, 1719, 1800
BciVI GTATCC 1 cut(s) 2378
BclI TGATCA 2 cut(s) 586, 2325
BcnI CCSGG 1 cut(s) 283
BcoDI GTCTC 5 cut(s) 237, 1040, 1144, 1928, 1952
BfaI CTAG 6 cut(s) 257, 948, 966, 1878, 2244, 2691
BfmI CTRYAG 2 cut(s) 1580, 2605
BfoI RGCGCY 1 cut(s) 2754
BfrI CTTAAG 1 cut(s) 1967
BfuI GTATCC 1 cut(s) 2378
BisI GCNGC 4 cut(s) 103, 904, 1583, 2319
BlsI GCNGC 4 cut(s) 104, 905, 1584, 2320
Bme1390I CCNGG 6 cut(s) 70, 164, 283, 1181, 1719, 1800
Bme18I GGWCC 3 cut(s) 742, 2363, 2599
BmeT110I CYCGRG 1 cut(s) 2734
BmgT120I GGNCC 7 cut(s) 211, 321, 344, 742, 1743, 2363, 2599
BmiI GGNNCC 5 cut(s) 74, 613, 897, 2124, 2214
BmrFI CCNGG 6 cut(s) 70, 164, 283, 1181, 1719, 1800
BpiI GAAGAC 1 cut(s) 1905
BplI GAGNNNNNCTC 2 cut(s) 113, 145
BpmI CTGGAG 2 cut(s) 1845, 2047
Bpu10I CCTNAGC 2 cut(s) 2059, 2810
Bpu14I TTCGAA 1 cut(s) 431
BpuEI CTTGAG 1 cut(s) 2414
BpuMI CCSGG 1 cut(s) 283
Bsa29I ATCGAT 2 cut(s) 518, 543
BsaBI GATNNNNATC 1 cut(s) 1770
BsaHI GRCGYC 1 cut(s) 1297
BsaI GGTCTC 4 cut(s) 1040, 1144, 1928, 1952
BsaJI CCNNGG 4 cut(s) 282, 2137, 2735, 2754
BsaWI WCCGGW 3 cut(s) 676, 1327, 2395
BsaXI ACNNNNNCTCC 6 cut(s) 714, 744, 869, 899, 1818, 1848
Bsc4I CCNNNNNNNGG 6 cut(s) 2138, 2142, 2372, 2395, 2423, 2495
Bse118I RCCGGY 3 cut(s) 646, 676, 2450
Bse1I ACTGG 4 cut(s) 794, 1566, 2302, 2594
Bse21I CCTNAGG 2 cut(s) 1317, 1605
Bse3DI GCAATG 2 cut(s) 2181, 2308
Bse8I GATNNNNATC 1 cut(s) 1770
BseAI TCCGGA 1 cut(s) 2395
BseBI CCWGG 5 cut(s) 70, 164, 1181, 1719, 1800
BseCI ATCGAT 2 cut(s) 518, 543
BseDI CCNNGG 4 cut(s) 282, 2137, 2735, 2754
BseGI GGATG 9 cut(s) 183, 292, 501, 695, 808, 1503, 2086, 2132, 2260
BseJI GATNNNNATC 1 cut(s) 1770
BseLI CCNNNNNNNGG 6 cut(s) 2138, 2142, 2372, 2395, 2423, 2495
BseMI GCAATG 2 cut(s) 2181, 2308
BseMII CTCAG 6 cut(s) 42, 1596, 2073, 2399, 2792, 2801
BseNI ACTGG 4 cut(s) 794, 1566, 2302, 2594
BseRI GAGGAG 6 cut(s) 77, 611, 620, 888, 890, 1099
BseSI GKGCMC 3 cut(s) 614, 1414, 1454
BseX3I CGGCCG 1 cut(s) 100
BseXI GCAGC 3 cut(s) 915, 1594, 2330
BseYI CCCAGC 1 cut(s) 933
BsgI GTGCAG 5 cut(s) 1265, 2070, 2273, 2337, 2631
Bsh1236I CGCG 3 cut(s) 190, 406, 2580
Bsh1285I CGRYCG 2 cut(s) 103, 2578
BshNI GGYRCC 2 cut(s) 72, 611
BshTI ACCGGT 1 cut(s) 676
BshVI ATCGAT 2 cut(s) 518, 543
BsiEI CGRYCG 2 cut(s) 103, 2578
BsiHKAI GWGCWC 4 cut(s) 126, 2154, 2395, 2660
BsiHKCI CYCGRG 1 cut(s) 2734
BsiSI CCGG 8 cut(s) 282, 350, 647, 677, 1328, 2396, 2451, 2669
BslI CCNNNNNNNGG 6 cut(s) 2138, 2142, 2372, 2395, 2423, 2495
BsmAI GTCTC 5 cut(s) 237, 1040, 1144, 1928, 1952
BsmBI CGTCTC 1 cut(s) 237
Bso31I GGTCTC 4 cut(s) 1040, 1144, 1928, 1952
BsoBI CYCGRG 1 cut(s) 2734
Bsp119I TTCGAA 1 cut(s) 431
Bsp1286I GDGCHC 7 cut(s) 126, 614, 1414, 1454, 2154, 2395, 2660
Bsp13I TCCGGA 1 cut(s) 2395
Bsp143I GATC 9 cut(s) 401, 586, 1053, 1321, 1963, 2325, 2411, 2500, 2704
BspCNI CTCAG 6 cut(s) 41, 1597, 2072, 2400, 2793, 2802
BspDI ATCGAT 2 cut(s) 518, 543
BspEI TCCGGA 1 cut(s) 2395
BspFNI CGCG 3 cut(s) 190, 406, 2580
BspHI TCATGA 1 cut(s) 2472
BspLI GGNNCC 5 cut(s) 74, 613, 897, 2124, 2214
BspMAI CTGCAG 1 cut(s) 1584
BspPI GGATC 3 cut(s) 1329, 2406, 2495
BspQI GCTCTTC 1 cut(s) 2790
BspT104I TTCGAA 1 cut(s) 431
BspT107I GGYRCC 2 cut(s) 72, 611
BspTI CTTAAG 1 cut(s) 1967
BspTNI GGTCTC 4 cut(s) 1040, 1144, 1928, 1952
BsrDI GCAATG 2 cut(s) 2181, 2308
BsrFI RCCGGY 3 cut(s) 646, 676, 2450
BsrI ACTGG 4 cut(s) 794, 1566, 2302, 2594
BssAI RCCGGY 3 cut(s) 646, 676, 2450
BssECI CCNNGG 4 cut(s) 282, 2137, 2735, 2754
BssMI GATC 9 cut(s) 401, 586, 1053, 1321, 1963, 2325, 2411, 2500, 2704
BssNI GRCGYC 1 cut(s) 1297
BssT1I CCWWGG 1 cut(s) 2137
Bst2UI CCWGG 5 cut(s) 70, 164, 1181, 1719, 1800
Bst4CI ACNGT 4 cut(s) 508, 1402, 1416, 2728
Bst6I CTCTTC 3 cut(s) 387, 876, 2790
BstACI GRCGYC 1 cut(s) 1297
BstAFI CTTAAG 1 cut(s) 1967
BstBI TTCGAA 1 cut(s) 431
BstC8I GCNNGC 5 cut(s) 126, 215, 1274, 1454, 1458
BstDEI CTNAG 9 cut(s) 28, 1168, 1317, 1605, 1908, 2059, 2408, 2801, 2810
BstEII GGTNACC 1 cut(s) 1754
BstF5I GGATG 9 cut(s) 183, 292, 501, 695, 808, 1503, 2086, 2132, 2260
BstFNI CGCG 3 cut(s) 190, 406, 2580
BstH2I RGCGCY 1 cut(s) 2754
BstHHI GCGC 2 cut(s) 192, 2753
BstKTI GATC 9 cut(s) 404, 589, 1056, 1324, 1966, 2328, 2414, 2503, 2707
BstMAI GTCTC 5 cut(s) 237, 1040, 1144, 1928, 1952
BstMBI GATC 9 cut(s) 401, 586, 1053, 1321, 1963, 2325, 2411, 2500, 2704
BstMCI CGRYCG 2 cut(s) 103, 2578
BstMWI GCNNNNNNNGC 9 cut(s) 245, 287, 1395, 1591, 1600, 1795, 2759, 2806, 2821
BstNI CCWGG 5 cut(s) 70, 164, 1181, 1719, 1800
BstNSI RCATGY 3 cut(s) 1276, 1280, 1460
BstPI GGTNACC 1 cut(s) 1754
BstSCI CCNGG 6 cut(s) 68, 162, 281, 1179, 1717, 1798
BstSFI CTRYAG 2 cut(s) 1580, 2605
BstSLI GKGCMC 3 cut(s) 614, 1414, 1454
BstUI CGCG 3 cut(s) 190, 406, 2580
BstV1I GCAGC 3 cut(s) 915, 1594, 2330
BstV2I GAAGAC 1 cut(s) 1905
BstX2I RGATCY 1 cut(s) 2411
BstYI RGATCY 1 cut(s) 2411
BstZI CGGCCG 1 cut(s) 100
Bsu15I ATCGAT 2 cut(s) 518, 543
Bsu36I CCTNAGG 2 cut(s) 1317, 1605
BsuI GTATCC 1 cut(s) 2378
BsuTUI ATCGAT 2 cut(s) 518, 543
BtgZI GCGATG 1 cut(s) 138
BtsCI GGATG 9 cut(s) 183, 292, 501, 695, 808, 1503, 2086, 2132, 2260
BtsI GCAGTG 5 cut(s) 283, 297, 754, 1217, 2548
BtsIMutI CAGTG 8 cut(s) 283, 297, 754, 1217, 1398, 2548, 2601, 2733
Cac8I GCNNGC 5 cut(s) 126, 215, 1274, 1454, 1458
CaiI CAGNNNCTG 2 cut(s) 1070, 2824
CciI TCATGA 1 cut(s) 2472
CfoI GCGC 2 cut(s) 192, 2753
Cfr10I RCCGGY 3 cut(s) 646, 676, 2450
Cfr13I GGNCC 7 cut(s) 211, 321, 344, 742, 1743, 2363, 2599
ClaI ATCGAT 2 cut(s) 518, 543
CseI GACGC 1 cut(s) 1305
CsiI ACCWGGT 1 cut(s) 162
Csp6I GTAC 7 cut(s) 73, 731, 1577, 1648, 1700, 1874, 2165
CspAI ACCGGT 1 cut(s) 676
CviQI GTAC 7 cut(s) 73, 731, 1577, 1648, 1700, 1874, 2165
DdeI CTNAG 9 cut(s) 28, 1168, 1317, 1605, 1908, 2059, 2408, 2801, 2810
DpnI GATC 9 cut(s) 403, 588, 1055, 1323, 1965, 2327, 2413, 2502, 2706
DpnII GATC 9 cut(s) 401, 586, 1053, 1321, 1963, 2325, 2411, 2500, 2704
DraI TTTAAA 1 cut(s) 1614
EaeI YGGCCR 1 cut(s) 100
EagI CGGCCG 1 cut(s) 100
Eam1104I CTCTTC 3 cut(s) 387, 876, 2790
EarI CTCTTC 3 cut(s) 387, 876, 2790
Ecl136II GAGCTC 3 cut(s) 124, 2152, 2393
EclXI CGGCCG 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 2137
Eco147I AGGCCT 3 cut(s) 68, 1603, 2427
Eco24I GRGCYC 3 cut(s) 126, 2154, 2395
Eco31I GGTCTC 4 cut(s) 1040, 1144, 1928, 1952
Eco32I GATATC 2 cut(s) 1869, 2440
Eco47I GGWCC 3 cut(s) 742, 2363, 2599
Eco52I CGGCCG 1 cut(s) 100
Eco53kI GAGCTC 3 cut(s) 124, 2152, 2393
Eco57I CTGAAG 2 cut(s) 1874, 2088
Eco81I CCTNAGG 2 cut(s) 1317, 1605
Eco88I CYCGRG 1 cut(s) 2734
Eco91I GGTNACC 1 cut(s) 1754
EcoICRI GAGCTC 3 cut(s) 124, 2152, 2393
EcoO109I RGGNCCY 1 cut(s) 2363
EcoO65I GGTNACC 1 cut(s) 1754
EcoRII CCWGG 5 cut(s) 68, 162, 1179, 1717, 1798
EcoRV GATATC 2 cut(s) 1869, 2440
EcoT14I CCWWGG 1 cut(s) 2137
EcoT22I ATGCAT 1 cut(s) 1278
EcoT38I GRGCYC 3 cut(s) 126, 2154, 2395
ErhI CCWWGG 1 cut(s) 2137
Esp3I CGTCTC 1 cut(s) 237
FauI CCCGC 3 cut(s) 157, 195, 1461
FauNDI CATATG 2 cut(s) 447, 1058
FbaI TGATCA 2 cut(s) 586, 2325
FblI GTMKAC 1 cut(s) 1039
Fnu4HI GCNGC 4 cut(s) 103, 904, 1583, 2319
FokI GGATG 9 cut(s) 170, 299, 508, 682, 815, 1510, 2093, 2119, 2267
FriOI GRGCYC 3 cut(s) 126, 2154, 2395
Fsp4HI GCNGC 4 cut(s) 103, 904, 1583, 2319
FspBI CTAG 6 cut(s) 257, 948, 966, 1878, 2244, 2691
GlaI GCGC 2 cut(s) 191, 2752
GluI GCNGC 4 cut(s) 103, 904, 1583, 2319
GsaI CCCAGC 1 cut(s) 937
GsuI CTGGAG 2 cut(s) 1845, 2047
HaeII RGCGCY 1 cut(s) 2754
HapII CCGG 8 cut(s) 282, 350, 647, 677, 1328, 2396, 2451, 2669
HgaI GACGC 1 cut(s) 1305
HhaI GCGC 2 cut(s) 192, 2753
Hin1I GRCGYC 1 cut(s) 1297
Hin6I GCGC 2 cut(s) 190, 2751
HinP1I GCGC 2 cut(s) 190, 2751
HincII GTYRAC 2 cut(s) 640, 1483
HindII GTYRAC 2 cut(s) 640, 1483
HindIII AAGCTT 2 cut(s) 2261, 2432
HpaI GTTAAC 2 cut(s) 640, 1483
HpaII CCGG 8 cut(s) 282, 350, 647, 677, 1328, 2396, 2451, 2669
HphI GGTGA 7 cut(s) 13, 104, 152, 520, 783, 884, 1169
Hpy166II GTNNAC 7 cut(s) 511, 640, 1040, 1405, 1483, 2599, 2731
Hpy8I GTNNAC 7 cut(s) 511, 640, 1040, 1405, 1483, 2599, 2731
HpyCH4III ACNGT 4 cut(s) 508, 1402, 1416, 2728
HpyCH4IV ACGT 4 cut(s) 729, 970, 1587, 2069
HpyF10VI GCNNNNNNNGC 9 cut(s) 245, 287, 1395, 1591, 1600, 1795, 2759, 2806, 2821
HpyF3I CTNAG 9 cut(s) 28, 1168, 1317, 1605, 1908, 2059, 2408, 2801, 2810
HpySE526I ACGT 4 cut(s) 729, 970, 1587, 2069
Hsp92I GRCGYC 1 cut(s) 1297
HspAI GCGC 2 cut(s) 190, 2751
Kpn2I TCCGGA 1 cut(s) 2395
KpnI GGTACC 1 cut(s) 76
Ksp22I TGATCA 2 cut(s) 586, 2325
KspAI GTTAAC 2 cut(s) 640, 1483
Kzo9I GATC 9 cut(s) 401, 586, 1053, 1321, 1963, 2325, 2411, 2500, 2704
LguI GCTCTTC 1 cut(s) 2790
Lsp1109I GCAGC 3 cut(s) 915, 1594, 2330
MabI ACCWGGT 1 cut(s) 162
MaeI CTAG 6 cut(s) 257, 948, 966, 1878, 2244, 2691
MaeII ACGT 4 cut(s) 729, 970, 1587, 2069
MaeIII GTNAC 4 cut(s) 223, 435, 1754, 2455
MalI GATC 9 cut(s) 403, 588, 1055, 1323, 1965, 2327, 2413, 2502, 2706
MboI GATC 9 cut(s) 401, 586, 1053, 1321, 1963, 2325, 2411, 2500, 2704
MflI RGATCY 1 cut(s) 2411
MhlI GDGCHC 7 cut(s) 126, 614, 1414, 1454, 2154, 2395, 2660
MlyI GAGTC 6 cut(s) 7, 20, 65, 103, 306, 2624
MmeI TCCRAC 3 cut(s) 397, 473, 847
Mph1103I ATGCAT 1 cut(s) 1278
MroI TCCGGA 1 cut(s) 2395
MroXI GAANNNNTTC 2 cut(s) 678, 2763
MslI CAYNNNNRTG 4 cut(s) 1281, 1461, 1707, 2279
MspCI CTTAAG 1 cut(s) 1967
MspI CCGG 8 cut(s) 282, 350, 647, 677, 1328, 2396, 2451, 2669
MspR9I CCNGG 6 cut(s) 70, 164, 283, 1181, 1719, 1800
MvaI CCWGG 5 cut(s) 70, 164, 1181, 1719, 1800
MvnI CGCG 3 cut(s) 190, 406, 2580
MwoI GCNNNNNNNGC 9 cut(s) 245, 287, 1395, 1591, 1600, 1795, 2759, 2806, 2821
NciI CCSGG 1 cut(s) 283
NdeI CATATG 2 cut(s) 447, 1058
NdeII GATC 9 cut(s) 401, 586, 1053, 1321, 1963, 2325, 2411, 2500, 2704
NlaIV GGNNCC 5 cut(s) 74, 613, 897, 2124, 2214
NmeAIII GCCGAG 2 cut(s) 78, 2779
NmuCI GTSAC 3 cut(s) 223, 435, 2455
NsiI ATGCAT 1 cut(s) 1278
NspI RCATGY 3 cut(s) 1276, 1280, 1460
NspV TTCGAA 1 cut(s) 431
PaeI GCATGC 2 cut(s) 1276, 1460
PagI TCATGA 1 cut(s) 2472
PceI AGGCCT 3 cut(s) 68, 1603, 2427
PciSI GCTCTTC 1 cut(s) 2790
PdmI GAANNNNTTC 2 cut(s) 678, 2763
PfeI GAWTC 6 cut(s) 171, 843, 984, 1355, 2182, 2230
PinAI ACCGGT 1 cut(s) 676
PkrI GCNGC 4 cut(s) 104, 905, 1584, 2320
PleI GAGTC 6 cut(s) 7, 20, 64, 103, 305, 2623
PpsI GAGTC 6 cut(s) 7, 20, 64, 103, 305, 2623
PpuMI RGGWCCY 1 cut(s) 2363
PshBI ATTAAT 1 cut(s) 365
Psp124BI GAGCTC 3 cut(s) 126, 2154, 2395
Psp5II RGGWCCY 1 cut(s) 2363
Psp6I CCWGG 5 cut(s) 68, 162, 1179, 1717, 1798
PspEI GGTNACC 1 cut(s) 1754
PspFI CCCAGC 1 cut(s) 933
PspGI CCWGG 5 cut(s) 68, 162, 1179, 1717, 1798
PspN4I GGNNCC 5 cut(s) 74, 613, 897, 2124, 2214
PspPI GGNCC 7 cut(s) 211, 321, 344, 742, 1743, 2363, 2599
PspPPI RGGWCCY 1 cut(s) 2363
PstI CTGCAG 1 cut(s) 1584
PstNI CAGNNNCTG 2 cut(s) 1070, 2824
PsuI RGATCY 1 cut(s) 2411
RsaI GTAC 7 cut(s) 74, 732, 1578, 1649, 1701, 1875, 2166
RsaNI GTAC 7 cut(s) 73, 731, 1577, 1648, 1700, 1874, 2165
RseI CAYNNNNRTG 4 cut(s) 1281, 1461, 1707, 2279
SacI GAGCTC 3 cut(s) 126, 2154, 2395
SapI GCTCTTC 1 cut(s) 2790
SatI GCNGC 4 cut(s) 103, 904, 1583, 2319
Sau3AI GATC 9 cut(s) 401, 586, 1053, 1321, 1963, 2325, 2411, 2500, 2704
Sau96I GGNCC 7 cut(s) 211, 321, 344, 742, 1743, 2363, 2599
SchI GAGTC 6 cut(s) 7, 20, 65, 103, 306, 2624
ScrFI CCNGG 6 cut(s) 70, 164, 283, 1181, 1719, 1800
SduI GDGCHC 7 cut(s) 126, 614, 1414, 1454, 2154, 2395, 2660
SexAI ACCWGGT 1 cut(s) 162
SfcI CTRYAG 2 cut(s) 1580, 2605
SfuI TTCGAA 1 cut(s) 431
SinI GGWCC 3 cut(s) 742, 2363, 2599
SmiMI CAYNNNNRTG 4 cut(s) 1281, 1461, 1707, 2279
SmlI CTYRAG 2 cut(s) 1967, 2429
SmoI CTYRAG 2 cut(s) 1967, 2429
SphI GCATGC 2 cut(s) 1276, 1460
SseBI AGGCCT 3 cut(s) 68, 1603, 2427
SspMI CTAG 6 cut(s) 257, 948, 966, 1878, 2244, 2691
SstI GAGCTC 3 cut(s) 126, 2154, 2395
StuI AGGCCT 3 cut(s) 68, 1603, 2427
StyD4I CCNGG 6 cut(s) 68, 162, 281, 1179, 1717, 1798
StyI CCWWGG 1 cut(s) 2137
TaaI ACNGT 4 cut(s) 508, 1402, 1416, 2728
TaiI ACGT 4 cut(s) 732, 973, 1590, 2072
TaqI TCGA 6 cut(s) 431, 518, 543, 1738, 2185, 2767
TatI WGTACW 3 cut(s) 1576, 1699, 2164
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 6 cut(s) 171, 843, 984, 1355, 2182, 2230
TscAI CASTG 8 cut(s) 283, 297, 754, 1217, 1405, 2548, 2601, 2733
TseFI GTSAC 3 cut(s) 223, 435, 2455
TseI GCWGC 3 cut(s) 903, 1582, 2318
Tsp45I GTSAC 3 cut(s) 223, 435, 2455
TspGWI ACGGA 2 cut(s) 1273, 1796
TspRI CASTG 8 cut(s) 283, 297, 754, 1217, 1405, 2548, 2601, 2733
Vha464I CTTAAG 1 cut(s) 1967
VpaK11BI GGWCC 3 cut(s) 742, 2363, 2599
VspI ATTAAT 1 cut(s) 365
XapI RAATTY 3 cut(s) 925, 1073, 1340
XceI RCATGY 3 cut(s) 1276, 1280, 1460
XmiI GTMKAC 1 cut(s) 1039
XmnI GAANNNNTTC 2 cut(s) 678, 2763
XspI CTAG 6 cut(s) 257, 948, 966, 1878, 2244, 2691
Zsp2I ATGCAT 1 cut(s) 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.