Rh7DG226800

serine threonine-protein kinase cdc7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0002088
Physical Location & Seq
Forward (+)
22319968 .. 22326457
6490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG226800.1

Sequence Viewer

Length: 3606 bp
ATGGCAACTAATCCACTCGACTCAGCCCTCACCGCCTCAACCATCTCCGACTCCGACAAGGCTTGGCACCTCCTCGCCCTCCTCTTATCTCTCGGCCGCCGCACCCCGCCCTCTGAGCTCGCCGCCCGATGCACGGCGTTCCCAGCAACTACTCATTCGGTCCGACGCCTTTGCTCCGTCGCCAATTCGCCGCTCTCCTTGTCCGACGATCTATACGTCACGCCGTCTCTACTCGCCTTAACCGCTCTCGCGGAGTCCGTTTCATGTTCCGGCGCCGCCGGCGGTGCACTGAGAACCTACTCAGGGAAGAGGAAGCGCGTCGTTTTGGATTGCGGATTTGTGCCCTCGGCCAAACGGAGATTGATTGTGGATTTTGAAAATGTATATGAAGAGAAAGGAGCGCGGAAATCAGTCGTAATTTCTCCTCCAAAGATACATTTTCATATGACTGACTACCTCAGCAGAAAAATAAATATGCTACCAAGCGATGTGGCTGTGGGGTTGAATAAACCGATGTTCAGTTCTCCATTATTTATTGAATATAGTGCTGAGCCATCCAGTGGTGGAGTGAAGAACATTGATAATGTAGAAGATCAAATGGAGACCGATCTAACTCTGCAAAGGAACATTAACAAAACAGTTATGGCCTGTGTAGATGATTTAGAGCCAACTGTTCCAACAGTGTCAGAAGATTTAGTACTTTATGAAGAAGCTAACATGCACGAGATAGATAGCTTGGCCAAATCTTACCTAGAGAAAAGTAAAGAAAGCATTTTGTCACCTGTGCTGGATGAGAATGTTAGCGGTGGTTGTAAAGTTGCAAATTCTACAATCCTTGAAGATGAAAGAATTGTGGCAGATTCGGAGGAAGAATTGTTAGTAGATCCAGGCAACCGCAATGGTGAAAGTGAAAACATGTCCCACCCACAAACTGCTCTCTCTGATAAGCTGCCTGGAATCGACTCCAGAAGATTAGAAGAGGAGGACTATGAAGATACAATACCTTTGAGAAAAAGGCTTATAAGAATACTTGAGACCCGAGTCAAACTTCCAGTTACTGATTTCTCTGTTGCTGAAAAGCAATTGTTGCAGCCATTTGAAAAGGTGTCCAAGGATTTAGTACTCTATGAAAGAACTAACATGCATGAGAAAACCAAAGAAAGCATAATCTCACCAGCTTTGGATGAGAGTGTTAGCGGTAGTCGTAAAGTTACAAACGCTACAATTCTTGAAGATGTAAGAACTGTGGGAAATCATGAGGAAGAACTGTTCATAGATCCTGGCAACCCTAATGGAGAGACTGAGAATCTATCACAGCCATTTGCTGCTGTTTCTGATAAGCAGCCTGGAACCGGCTCCAGAAGCTTAAAAGAGCAGGATTTTGAAGATACGATACCATTGCATAAAGAGCGACATGAGAACCAAACCATTTGTGTCGCCACTGATCTCTCCTCTCCTAAGGTGTCCAATGATTTAGTACTCTATGAAGGAGCTGACATGCATGAGATAGATATCCAGGGAAACTCTTACCAAGAGAAAATCAAAGAAAGCATAATGCCAGCTTTGGATGAGAGTGTTAGTGGTAACTGTAAAGGTACAAATGCTACAATGCTTGAAGATAAACGAATTGTGGGAGATGCAGAGGAAGGATTGTTAGTAAATCCTGGCAACCCTATTGGAGAAACAGAAATTCTATACCAGCCATATACTAATGTTTCTGATAAGCTGGCTGGAATTGACTTCAGAAGATTGAATGAGGATGATTATGAAGATACAACACCTGTGAATAAAAGTCTTATGACAAGAAATGAGACCCAAACCACCTGTCCTGCTACTGAATTCTATGTTCCTCAAAAGCAGTTGTTGAAGTCCTCTGCAACAATGAAGGCCATGGACAAGGATAAAATATCTTCAAGATTGCAATCTCTATATAGTTGTAGTCTAGAGCAAGATCAGACTGTTGCCACTCCCAAAGTGAACCTCCAACATAAAATGGATCAGAAGACAAATAGCATGGTACACAAATCAAAGCAGAATTGTGGTGACATGCATATTAATGAAAGAAAGAGACGATACAAATCTATTTCCTCTAAGGATCAGCCAGAGAAAAAAGAACTTCCAAATTTTGATTCTTATATGGTAGAAGAAGAAGAAGGTTCAGGTGGTTATGGCACTGTTTACAGGGCGAGGAGTAAGAATGACAGGACAAGGGTTGCCATTAAGTGTCCACATGCTAATGCTCATAAGAGCCATGTTAACAATGAGTTGAGAATGCTGGAAAGGTTTGGGGGGAAGAACTTTGTGATCAAATATGAAGGCTGTATCAAGAATGGAAATTCAAGTTGCTTTGTATTACAGCATGTTGAGCATGATAGGCCTGAGGTTTTAAAGAAAGAGATAGATGTTTGTGAGCTCCGGTGGTATGGATATTGCTTGTTTAGAGCACTTGCAAGCCTTCATAAGCAGGGAATCATACATAGAGATGTTAAACCCGGCAATTTCCTTTTCTCTCGTAAGGCCAACAAGGGTTACCTTATTGATTTCAATCTATCCATGGACTTGCATCAGAAGTATGGAAATAATAGTAAGTCAAGAGGGGGATTTGATGTAAGCAGCAATGATGAGAAGCTTATGACTGCAAAAAATTTACCTCCAATCACACTTAGGAAGTTGCCAAGTGGTAAATCTTTACTAACAGCCAATGGAGAGACCTCAAAAGGTTTGAAATCCACTTTTGATATGAAGGATCTAAAAAAGATGGCTTTCAGCCAAATGAAGGCCAATAATGATTCGGGTAGTAGGAAGGTAATCAAAAGTCAGGGTGCAGATGGCTCAGGCATAACGTCAGTAAAGGATGTTACAAGTACCAGAACTCCTTCGGCAGAAAGGATGAGAGAACCTTTGCCATGCCAAGGAAGGAAAGAACTGATCAGCCTAGTACAGGAAGCAAGGCAGAGTCCAAATCATGAGGCATCAAGTATTTCAGCTCTTATGAGAAAGAGGGTTCCTGCCCCTCCCGTAAATGTTGATGGCAAGCTTTTCCATGTCACTCCGATGCCTATGCACTCAACTACCAGTGGTATTGGTGGTCCATGCTTGATAAGAAGCAGAGGTCTTGGAAAACATAAAGAAGGCCCTTGTGCTGGAACAAAAGGCTTCAGGGCTCCAGAGGTTCTCTTCAGATCTCCACATCAAGGCCCCAAAATTGATATCTGGTCTGCCGGAGTCACGCTACTCTACTTGTTGATTGGCAGAACACCCTTCCTTGGTGAACCTGAACTGAACATCAAGGATATAGCAAAGCTGAGAGGCAGTGAAGATTTATGGGAAGTTGCCAAGCTACACGACCGTGAATCCTCATTTCCAGTGGAGCTCTATAGTACAGATTGTTTGCCCTCTATTAAACTGAAGGAGTGGTGTAGAATGAACACAAAGAGACCGGACTTCTTTGAGCAAATCCCAAGACCCCTTTTCGATCTTGTGGATAAATGCTTGATGGTGAACCCTAGGGTTAGATTGAGTGCAGAGGAAGCGCTTAAACATGAGTTTTTCGCTCCATGCCATGAAGAACTGAGAAAGCACAGGCTTCAAAGGCAGGTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006950 GO:0006974 GO:0007049 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0008360 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010389 GO:0010556 GO:0010557 GO:0010564 GO:0010571 GO:0010604 GO:0010948 GO:0010971 GO:0015630 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0033262 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045171 GO:0045740 GO:0045786 GO:0045787 GO:0045931 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070316 GO:0070317 GO:0071704 GO:0072686 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0098657 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1903047 GO:2000105 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 map04110 map04111 map04113
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1201

Amino Acids

134.07

Weight (kDa)

6.56

Isoelectric Point (pI)

49.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 713 - 858 6.3e-23 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 716 - 861 1.6e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 1048 - 1184 4e-16 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1022
Acc36I ACCTGC 1 cut(s) 3586
AccB1I GGYRCC 2 cut(s) 66, 272
AccB7I CCANNNNNTGG 1 cut(s) 560
AccBSI CCGCTC 2 cut(s) 193, 245
AccII CGCG 3 cut(s) 251, 318, 403
AclWI GGATC 5 cut(s) 878, 1271, 2004, 2103, 2754
AcoI YGGCCR 3 cut(s) 94, 348, 738
AcsI RAATTY 6 cut(s) 823, 1689, 1838, 2122, 2335, 2644
AcuI CTGAAG 4 cut(s) 1726, 3142, 3163, 3428
AcyI GRCGYC 2 cut(s) 166, 273
AfaI GTAC 8 cut(s) 699, 1122, 1479, 1597, 2019, 2866, 2940, 3382
AfeI AGCGCT 1 cut(s) 3534
AflIII ACRYGT 1 cut(s) 915
AjnI CCWGG 6 cut(s) 886, 952, 1279, 1345, 1515, 1663
AjuI GAANNNNNNNTTGG 2 cut(s) 2113, 2145
AleI CACNNNNGTG 1 cut(s) 3348
AloI GAACNNNNNNTCC 2 cut(s) 1253, 1285
Alw21I GWGCWC 5 cut(s) 120, 289, 2415, 2446, 3375
Alw26I GTCTC 8 cut(s) 231, 596, 1028, 1292, 1805, 2062, 2702, 3430
Alw44I GTGCAC 1 cut(s) 285
AlwI GGATC 5 cut(s) 878, 1271, 2004, 2103, 2754
AlwNI CAGNNNCTG 1 cut(s) 1058
Ama87I CYCGRG 1 cut(s) 1038
Aor51HI AGCGCT 1 cut(s) 3534
ApaLI GTGCAC 1 cut(s) 285
ApeKI GCWGC 5 cut(s) 949, 1090, 1325, 1342, 2613
ApoI RAATTY 6 cut(s) 823, 1689, 1838, 2122, 2335, 2644
ArsI GACNNNNNNTTYG 2 cut(s) 1810, 1842
AseI ATTAAT 1 cut(s) 2055
Asp700I GAANNNNTTC 1 cut(s) 2875
AspA2I CCTAGG 1 cut(s) 3506
AspLEI GCGC 4 cut(s) 275, 318, 403, 3535
AspS9I GGNCC 4 cut(s) 160, 3089, 3134, 3197
AsuC2I CCSGG 1 cut(s) 2493
AsuHPI GGTGA 7 cut(s) 22, 771, 914, 1164, 2054, 3281, 3511
AvaI CYCGRG 1 cut(s) 1038
AvaII GGWCC 2 cut(s) 160, 3089
AvrII CCTAGG 1 cut(s) 3506
AxyI CCTNAGG 2 cut(s) 1458, 2379
BaeGI GKGCMC 2 cut(s) 289, 345
BalI TGGCCA 1 cut(s) 740
BanI GGYRCC 2 cut(s) 66, 272
BanII GRGCYC 4 cut(s) 120, 2415, 3166, 3375
BarI GAAGNNNNNNTAC 6 cut(s) 681, 713, 984, 1016, 1377, 1409
BauI CACGAG 1 cut(s) 722
BbsI GAAGAC 1 cut(s) 2009
Bbv12I GWGCWC 5 cut(s) 120, 289, 2415, 2446, 3375
BbvCI CCTCAGC 1 cut(s) 458
BbvI GCAGC 5 cut(s) 936, 1102, 1312, 1354, 2625
BccI CCATC 6 cut(s) 50, 562, 2752, 2822, 3023, 3490
BceAI ACGGC 2 cut(s) 150, 208
BcgI CGANNNNNNTGC 6 cut(s) 153, 187, 1381, 1415, 3043, 3077
BciT130I CCWGG 6 cut(s) 888, 954, 1281, 1347, 1517, 1665
BclI TGATCA 2 cut(s) 2304, 2928
BcnI CCSGG 1 cut(s) 2493
BcoDI GTCTC 8 cut(s) 231, 596, 1028, 1292, 1805, 2062, 2702, 3430
BfaI CTAG 4 cut(s) 752, 1943, 2936, 3507
BfmI CTRYAG 1 cut(s) 3376
BfoI RGCGCY 2 cut(s) 276, 3536
BfuAI ACCTGC 1 cut(s) 3586
BglII AGATCT 1 cut(s) 3182
BlnI CCTAGG 1 cut(s) 3506
BlpI GCTNAGC 1 cut(s) 549
BmcAI AGTACT 3 cut(s) 699, 1122, 1479
Bme1390I CCNGG 7 cut(s) 888, 954, 1281, 1347, 1517, 1665, 2493
Bme18I GGWCC 2 cut(s) 160, 3089
BmeT110I CYCGRG 1 cut(s) 1038
BmgT120I GGNCC 4 cut(s) 160, 3089, 3134, 3197
BmiI GGNNCC 7 cut(s) 68, 274, 1351, 1357, 3006, 3165, 3199
BmrFI CCNGG 7 cut(s) 888, 954, 1281, 1347, 1517, 1665, 2493
BmsI GCATC 5 cut(s) 119, 1627, 2572, 2981, 3045
BoxI GACNNNNGTC 1 cut(s) 1040
BpiI GAAGAC 1 cut(s) 2009
BpmI CTGGAG 3 cut(s) 949, 1342, 3150
Bpu10I CCTNAGC 2 cut(s) 458, 2833
Bpu1102I GCTNAGC 1 cut(s) 549
BpuEI CTTGAG 1 cut(s) 1052
BpuMI CCSGG 1 cut(s) 2493
BsaBI GATNNNNATC 4 cut(s) 1511, 1921, 2077, 2544
BsaHI GRCGYC 2 cut(s) 166, 273
BsaI GGTCTC 5 cut(s) 596, 1028, 1805, 2702, 3430
BsaJI CCNNGG 8 cut(s) 345, 1110, 1516, 1890, 2553, 2911, 3265, 3506
BsaWI WCCGGW 2 cut(s) 2415, 3439
BsaXI ACNNNNNCTCC 2 cut(s) 2857, 2887
Bse118I RCCGGY 2 cut(s) 278, 1352
Bse1I ACTGG 4 cut(s) 558, 1052, 3075, 3365
Bse21I CCTNAGG 2 cut(s) 1458, 2379
Bse3DI GCAATG 3 cut(s) 904, 1397, 2623
Bse8I GATNNNNATC 4 cut(s) 1511, 1921, 2077, 2544
BseBI CCWGG 6 cut(s) 888, 954, 1281, 1347, 1517, 1665
BseDI CCNNGG 8 cut(s) 345, 1110, 1516, 1890, 2553, 2911, 3265, 3506
BseGI GGATG 7 cut(s) 554, 796, 1189, 1573, 1765, 2860, 2895
BseJI GATNNNNATC 4 cut(s) 1511, 1921, 2077, 2544
BseMI GCAATG 3 cut(s) 904, 1397, 2623
BseNI ACTGG 4 cut(s) 558, 1052, 3075, 3365
BseRI GAGGAG 6 cut(s) 62, 71, 414, 995, 1441, 2203
BseSI GKGCMC 2 cut(s) 289, 345
BseX3I CGGCCG 1 cut(s) 94
BseXI GCAGC 5 cut(s) 936, 1102, 1312, 1354, 2625
BseYI CCCAGC 1 cut(s) 142
BsgI GTGCAG 2 cut(s) 2844, 3543
Bsh1236I CGCG 3 cut(s) 251, 318, 403
Bsh1285I CGRYCG 2 cut(s) 97, 3349
BshNI GGYRCC 2 cut(s) 66, 272
BsiEI CGRYCG 2 cut(s) 97, 3349
BsiHKAI GWGCWC 5 cut(s) 120, 289, 2415, 2446, 3375
BsiHKCI CYCGRG 1 cut(s) 1038
BsiSI CCGG 7 cut(s) 270, 279, 1353, 2416, 2493, 3222, 3440
BslFI GGGAC 1 cut(s) 904
BsmAI GTCTC 8 cut(s) 231, 596, 1028, 1292, 1805, 2062, 2702, 3430
BsmBI CGTCTC 2 cut(s) 231, 2062
BsmFI GGGAC 1 cut(s) 904
BsmI GAATGC 1 cut(s) 2277
Bso31I GGTCTC 5 cut(s) 596, 1028, 1805, 2702, 3430
BsoBI CYCGRG 1 cut(s) 1038
Bsp1286I GDGCHC 7 cut(s) 120, 289, 345, 2415, 2446, 3166, 3375
Bsp1720I GCTNAGC 1 cut(s) 549
Bsp19I CCATGG 2 cut(s) 1890, 2553
BspFNI CGCG 3 cut(s) 251, 318, 403
BspHI TCATGA 2 cut(s) 1255, 2965
BspLI GGNNCC 7 cut(s) 68, 274, 1351, 1357, 3006, 3165, 3199
BspMI ACCTGC 1 cut(s) 3586
BspPI GGATC 5 cut(s) 878, 1271, 2004, 2103, 2754
BspT107I GGYRCC 2 cut(s) 66, 272
BspTNI GGTCTC 5 cut(s) 596, 1028, 1805, 2702, 3430
BsrBI CCGCTC 2 cut(s) 193, 245
BsrDI GCAATG 3 cut(s) 904, 1397, 2623
BsrFI RCCGGY 2 cut(s) 278, 1352
BsrI ACTGG 4 cut(s) 558, 1052, 3075, 3365
BssAI RCCGGY 2 cut(s) 278, 1352
BssECI CCNNGG 8 cut(s) 345, 1110, 1516, 1890, 2553, 2911, 3265, 3506
BssNI GRCGYC 2 cut(s) 166, 273
BssSI CACGAG 1 cut(s) 722
BssT1I CCWWGG 6 cut(s) 1110, 1890, 2553, 2911, 3265, 3506
Bst2BI CACGAG 1 cut(s) 722
Bst2UI CCWGG 6 cut(s) 888, 954, 1281, 1347, 1517, 1665
Bst4CI ACNGT 9 cut(s) 640, 673, 682, 1246, 1269, 1589, 1960, 2176, 3350
Bst6I CTCTTC 4 cut(s) 302, 384, 972, 3182
BstACI GRCGYC 2 cut(s) 166, 273
BstAPI GCANNNNNTGC 1 cut(s) 1087
BstC8I GCNNGC 6 cut(s) 120, 280, 1560, 1728, 2452, 3035
BstDSI CCRYGG 2 cut(s) 1890, 2553
BstEII GGTNACC 1 cut(s) 2528
BstENI CCTNNNNNAGG 1 cut(s) 2939
BstF5I GGATG 7 cut(s) 554, 796, 1189, 1573, 1765, 2860, 2895
BstFNI CGCG 3 cut(s) 251, 318, 403
BstH2I RGCGCY 2 cut(s) 276, 3536
BstHHI GCGC 4 cut(s) 275, 318, 403, 3535
BstMAI GTCTC 8 cut(s) 231, 596, 1028, 1292, 1805, 2062, 2702, 3430
BstMCI CGRYCG 2 cut(s) 97, 3349
BstNI CCWGG 6 cut(s) 888, 954, 1281, 1347, 1517, 1665
BstNSI RCATGY 7 cut(s) 721, 919, 1144, 1501, 2050, 2234, 2363
BstPAI GACNNNNGTC 1 cut(s) 1040
BstPI GGTNACC 1 cut(s) 2528
BstSCI CCNGG 7 cut(s) 886, 952, 1279, 1345, 1515, 1663, 2491
BstSFI CTRYAG 1 cut(s) 3376
BstSLI GKGCMC 2 cut(s) 289, 345
BstUI CGCG 3 cut(s) 251, 318, 403
BstV1I GCAGC 5 cut(s) 936, 1102, 1312, 1354, 2625
BstV2I GAAGAC 1 cut(s) 2009
BstX2I RGATCY 4 cut(s) 883, 1276, 2746, 3182
BstYI RGATCY 4 cut(s) 883, 1276, 2746, 3182
BstZI CGGCCG 1 cut(s) 94
Bsu36I CCTNAGG 2 cut(s) 1458, 2379
BtgI CCRYGG 2 cut(s) 1890, 2553
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 7 cut(s) 554, 796, 1189, 1573, 1765, 2860, 2895
BtsI GCAGTG 1 cut(s) 3319
BtsIMutI CAGTG 8 cut(s) 287, 565, 687, 1440, 2172, 3082, 3319, 3372
BveI ACCTGC 1 cut(s) 3586
Cac8I GCNNGC 6 cut(s) 120, 280, 1560, 1728, 2452, 3035
CaiI CAGNNNCTG 1 cut(s) 1058
CciI TCATGA 2 cut(s) 1255, 2965
CfoI GCGC 4 cut(s) 275, 318, 403, 3535
Cfr10I RCCGGY 2 cut(s) 278, 1352
Cfr13I GGNCC 4 cut(s) 160, 3089, 3134, 3197
CpoI CGGWCCG 1 cut(s) 160
CseI GACGC 2 cut(s) 174, 307
Csp6I GTAC 8 cut(s) 698, 1121, 1478, 1596, 2018, 2865, 2939, 3381
CspCI CAANNNNNGTGG 2 cut(s) 471, 506
CspI CGGWCCG 1 cut(s) 160
CviQI GTAC 8 cut(s) 698, 1121, 1478, 1596, 2018, 2865, 2939, 3381
DinI GGCGCC 1 cut(s) 274
DraI TTTAAA 1 cut(s) 2388
EaeI YGGCCR 3 cut(s) 94, 348, 738
EagI CGGCCG 1 cut(s) 94
Eam1104I CTCTTC 4 cut(s) 302, 384, 972, 3182
EarI CTCTTC 4 cut(s) 302, 384, 972, 3182
Ecl136II GAGCTC 3 cut(s) 118, 2413, 3373
EclXI CGGCCG 1 cut(s) 94
Eco130I CCWWGG 6 cut(s) 1110, 1890, 2553, 2911, 3265, 3506
Eco147I AGGCCT 1 cut(s) 2377
Eco24I GRGCYC 4 cut(s) 120, 2415, 3166, 3375
Eco31I GGTCTC 5 cut(s) 596, 1028, 1805, 2702, 3430
Eco32I GATATC 2 cut(s) 1513, 3211
Eco47I GGWCC 2 cut(s) 160, 3089
Eco47III AGCGCT 1 cut(s) 3534
Eco52I CGGCCG 1 cut(s) 94
Eco53kI GAGCTC 3 cut(s) 118, 2413, 3373
Eco57I CTGAAG 4 cut(s) 1726, 3142, 3163, 3428
Eco81I CCTNAGG 2 cut(s) 1458, 2379
Eco88I CYCGRG 1 cut(s) 1038
Eco91I GGTNACC 1 cut(s) 2528
EcoICRI GAGCTC 3 cut(s) 118, 2413, 3373
EcoNI CCTNNNNNAGG 1 cut(s) 2939
EcoO109I RGGNCCY 2 cut(s) 3134, 3197
EcoO65I GGTNACC 1 cut(s) 2528
EcoRI GAATTC 1 cut(s) 1838
EcoRII CCWGG 6 cut(s) 886, 952, 1279, 1345, 1515, 1663
EcoRV GATATC 2 cut(s) 1513, 3211
EcoT14I CCWWGG 6 cut(s) 1110, 1890, 2553, 2911, 3265, 3506
EcoT22I ATGCAT 3 cut(s) 1146, 1503, 2052
EcoT38I GRGCYC 4 cut(s) 120, 2415, 3166, 3375
EgeI GGCGCC 1 cut(s) 274
EheI GGCGCC 1 cut(s) 274
ErhI CCWWGG 6 cut(s) 1110, 1890, 2553, 2911, 3265, 3506
Esp3I CGTCTC 2 cut(s) 231, 2062
FaqI GGGAC 1 cut(s) 904
FauI CCCGC 1 cut(s) 114
FauNDI CATATG 1 cut(s) 444
FbaI TGATCA 2 cut(s) 2304, 2928
FokI GGATG 7 cut(s) 541, 803, 1196, 1580, 1772, 2867, 2902
FriOI GRGCYC 4 cut(s) 120, 2415, 3166, 3375
FspBI CTAG 4 cut(s) 752, 1943, 2936, 3507
GlaI GCGC 4 cut(s) 274, 317, 402, 3534
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 3 cut(s) 949, 1342, 3150
HaeII RGCGCY 2 cut(s) 276, 3536
HapII CCGG 7 cut(s) 270, 279, 1353, 2416, 2493, 3222, 3440
HgaI GACGC 2 cut(s) 174, 307
HhaI GCGC 4 cut(s) 275, 318, 403, 3535
Hin1I GRCGYC 2 cut(s) 166, 273
Hin6I GCGC 4 cut(s) 273, 316, 401, 3533
HinP1I GCGC 4 cut(s) 273, 316, 401, 3533
HincII GTYRAC 1 cut(s) 2257
HindII GTYRAC 1 cut(s) 2257
HindIII AAGCTT 3 cut(s) 1363, 2627, 3035
HpaI GTTAAC 1 cut(s) 2257
HpaII CCGG 7 cut(s) 270, 279, 1353, 2416, 2493, 3222, 3440
HphI GGTGA 7 cut(s) 22, 771, 914, 1164, 2054, 3281, 3511
Hpy166II GTNNAC 8 cut(s) 287, 1978, 2020, 2179, 2228, 2257, 3272, 3502
Hpy8I GTNNAC 8 cut(s) 287, 1978, 2020, 2179, 2228, 2257, 3272, 3502
Hpy99I CGWCG 4 cut(s) 168, 182, 209, 323
HpyCH4III ACNGT 9 cut(s) 640, 673, 682, 1246, 1269, 1589, 1960, 2176, 3350
HpyCH4IV ACGT 2 cut(s) 216, 2843
HpySE526I ACGT 2 cut(s) 216, 2843
Hsp92I GRCGYC 2 cut(s) 166, 273
HspAI GCGC 4 cut(s) 273, 316, 401, 3533
KasI GGCGCC 1 cut(s) 272
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Ksp22I TGATCA 2 cut(s) 2304, 2928
KspAI GTTAAC 1 cut(s) 2257
LmnI GCTCC 8 cut(s) 179, 398, 1361, 1490, 2418, 3169, 3370, 3559
Lsp1109I GCAGC 5 cut(s) 936, 1102, 1312, 1354, 2625
LweI GCATC 5 cut(s) 119, 1627, 2572, 2981, 3045
MaeI CTAG 4 cut(s) 752, 1943, 2936, 3507
MaeII ACGT 2 cut(s) 216, 2843
MbiI CCGCTC 2 cut(s) 193, 245
MfeI CAATTG 1 cut(s) 1082
MflI RGATCY 4 cut(s) 883, 1276, 2746, 3182
MhlI GDGCHC 7 cut(s) 120, 289, 345, 2415, 2446, 3166, 3375
MlsI TGGCCA 1 cut(s) 740
MluNI TGGCCA 1 cut(s) 740
Mly113I GGCGCC 1 cut(s) 273
MlyI GAGTC 7 cut(s) 14, 44, 263, 956, 1050, 2965, 3234
MmeI TCCRAC 6 cut(s) 72, 78, 187, 228, 701, 2008
Mox20I TGGCCA 1 cut(s) 740
Mph1103I ATGCAT 3 cut(s) 1146, 1503, 2052
MreI CGCCGGCG 1 cut(s) 278
MroNI GCCGGC 1 cut(s) 278
MroXI GAANNNNTTC 1 cut(s) 2875
MscI TGGCCA 1 cut(s) 740
MslI CAYNNNNRTG 5 cut(s) 2055, 2235, 2481, 3053, 3348
Msp20I TGGCCA 1 cut(s) 740
MspI CCGG 7 cut(s) 270, 279, 1353, 2416, 2493, 3222, 3440
MspR9I CCNGG 7 cut(s) 888, 954, 1281, 1347, 1517, 1665, 2493
MunI CAATTG 1 cut(s) 1082
Mva1269I GAATGC 1 cut(s) 2277
MvaI CCWGG 6 cut(s) 888, 954, 1281, 1347, 1517, 1665
MvnI CGCG 3 cut(s) 251, 318, 403
NaeI GCCGGC 1 cut(s) 280
NarI GGCGCC 1 cut(s) 273
NciI CCSGG 1 cut(s) 2493
NcoI CCATGG 2 cut(s) 1890, 2553
NdeI CATATG 1 cut(s) 444
NgoMIV GCCGGC 1 cut(s) 278
NlaIV GGNNCC 7 cut(s) 68, 274, 1351, 1357, 3006, 3165, 3199
NmeAIII GCCGAG 2 cut(s) 72, 326
NmuCI GTSAC 5 cut(s) 217, 777, 2042, 3046, 3226
NsiI ATGCAT 3 cut(s) 1146, 1503, 2052
NspI RCATGY 7 cut(s) 721, 919, 1144, 1501, 2050, 2234, 2363
OliI CACNNNNGTG 1 cut(s) 3348
PagI TCATGA 2 cut(s) 1255, 2965
PceI AGGCCT 1 cut(s) 2377
PciI ACATGT 1 cut(s) 915
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 255
PctI GAATGC 1 cut(s) 2277
PdiI GCCGGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 2875
PfeI GAWTC 7 cut(s) 860, 957, 1306, 2129, 2469, 2789, 3353
PflMI CCANNNNNTGG 1 cut(s) 560
PleI GAGTC 7 cut(s) 14, 44, 262, 956, 1049, 2964, 3233
PluTI GGCGCC 1 cut(s) 276
PpsI GAGTC 7 cut(s) 14, 44, 262, 956, 1049, 2964, 3233
PscI ACATGT 1 cut(s) 915
PshAI GACNNNNGTC 1 cut(s) 1040
PshBI ATTAAT 1 cut(s) 2055
PsiI TTATAA 1 cut(s) 1022
Psp124BI GAGCTC 3 cut(s) 120, 2415, 3375
Psp6I CCWGG 6 cut(s) 886, 952, 1279, 1345, 1515, 1663
PspEI GGTNACC 1 cut(s) 2528
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 6 cut(s) 886, 952, 1279, 1345, 1515, 1663
PspN4I GGNNCC 7 cut(s) 68, 274, 1351, 1357, 3006, 3165, 3199
PspPI GGNCC 4 cut(s) 160, 3089, 3134, 3197
PstNI CAGNNNCTG 1 cut(s) 1058
PsuI RGATCY 4 cut(s) 883, 1276, 2746, 3182
RsaI GTAC 8 cut(s) 699, 1122, 1479, 1597, 2019, 2866, 2940, 3382
RsaNI GTAC 8 cut(s) 698, 1121, 1478, 1596, 2018, 2865, 2939, 3381
RseI CAYNNNNRTG 5 cut(s) 2055, 2235, 2481, 3053, 3348
Rsr2I CGGWCCG 1 cut(s) 160
RsrII CGGWCCG 1 cut(s) 160
SacI GAGCTC 3 cut(s) 120, 2415, 3375
Sau96I GGNCC 4 cut(s) 160, 3089, 3134, 3197
ScaI AGTACT 3 cut(s) 699, 1122, 1479
SchI GAGTC 7 cut(s) 14, 44, 263, 956, 1050, 2965, 3234
ScrFI CCNGG 7 cut(s) 888, 954, 1281, 1347, 1517, 1665, 2493
SduI GDGCHC 7 cut(s) 120, 289, 345, 2415, 2446, 3166, 3375
SfaNI GCATC 5 cut(s) 119, 1627, 2572, 2981, 3045
SfcI CTRYAG 1 cut(s) 3376
SfoI GGCGCC 1 cut(s) 274
SgrAI CRCCGGYG 1 cut(s) 278
SinI GGWCC 2 cut(s) 160, 3089
SmiMI CAYNNNNRTG 5 cut(s) 2055, 2235, 2481, 3053, 3348
SmlI CTYRAG 1 cut(s) 1031
SmoI CTYRAG 1 cut(s) 1031
SseBI AGGCCT 1 cut(s) 2377
SspDI GGCGCC 1 cut(s) 272
SspMI CTAG 4 cut(s) 752, 1943, 2936, 3507
SstI GAGCTC 3 cut(s) 120, 2415, 3375
StuI AGGCCT 1 cut(s) 2377
StyD4I CCNGG 7 cut(s) 886, 952, 1279, 1345, 1515, 1663, 2491
StyI CCWWGG 6 cut(s) 1110, 1890, 2553, 2911, 3265, 3506
TaaI ACNGT 9 cut(s) 640, 673, 682, 1246, 1269, 1589, 1960, 2176, 3350
TaiI ACGT 2 cut(s) 219, 2846
TaqI TCGA 3 cut(s) 18, 960, 3474
TaqII GACCGA 2 cut(s) 148, 620
TatI WGTACW 5 cut(s) 697, 1120, 1477, 2938, 3380
TauI GCSGC 5 cut(s) 99, 102, 125, 193, 278
TfiI GAWTC 7 cut(s) 860, 957, 1306, 2129, 2469, 2789, 3353
TscAI CASTG 8 cut(s) 294, 565, 687, 1447, 2179, 3082, 3319, 3372
TseFI GTSAC 5 cut(s) 217, 777, 2042, 3046, 3226
TseI GCWGC 5 cut(s) 949, 1090, 1325, 1342, 2613
Tsp45I GTSAC 5 cut(s) 217, 777, 2042, 3046, 3226
TspGWI ACGGA 3 cut(s) 166, 247, 370
TspRI CASTG 8 cut(s) 294, 565, 687, 1447, 2179, 3082, 3319, 3372
Van91I CCANNNNNTGG 1 cut(s) 560
VneI GTGCAC 1 cut(s) 285
VpaK11BI GGWCC 2 cut(s) 160, 3089
VspI ATTAAT 1 cut(s) 2055
XagI CCTNNNNNAGG 1 cut(s) 2939
XapI RAATTY 6 cut(s) 823, 1689, 1838, 2122, 2335, 2644
XbaI TCTAGA 1 cut(s) 1942
XceI RCATGY 7 cut(s) 721, 919, 1144, 1501, 2050, 2234, 2363
XmaJI CCTAGG 1 cut(s) 3506
XmnI GAANNNNTTC 1 cut(s) 2875
XspI CTAG 4 cut(s) 752, 1943, 2936, 3507
ZrmI AGTACT 3 cut(s) 699, 1122, 1479
Zsp2I ATGCAT 3 cut(s) 1146, 1503, 2052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.