RchiOBHm_Chr7g0205281

serine threonine-protein kinase cdc7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002088
Physical Location & Seq
Forward (+)
22969358 .. 22976023
6666 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18370

Sequence Viewer

Length: 3732 bp
ATGGCGGGAACTTCTCACGCTCTCAATCTCAAACCCTACACGTGTCCCCGCATTGCCGCCTCTCTTTCCCCCTCAATTCTACATCTCTCTCTCTCTCTCCCCGATCCAAATCTCAGACCCACAGCCATGGCAACTAATCCACTCGACTCAGCCCTCACCGCCTCAACCATCTCCGACTCCGACAAGGCTTGGCACCTCCTCGCCCTCCTCTTATCTCTCGGCCGCCGCACCCCGCCCTCTGAGCTCGCCGCCCGATGCACGGCGTTCCCAGCAACTACTCATTCGGTCCGACGCCTTTGCTCCGTCGCCAATTCGCCGCTCTCCTTGTCCGACGATCTATACGTCACGCCGTCTCTACTCGCCTTAACCGCTCTCGCGGAGTCCGTTTCATGTTCCGGCGCCGCCGGCGGTGCACTGAGAACCTACTCAGGGAAGAGGAAGCGCGTCGTTTTGGATTGCGGATTTGTGCCCTCGGCCAAACGGAGATTGATTGTGGATTTTGAAAATGTATATGAAGAGAAAGGAGCGCGGAAATCAGTCGTAATTTCTCCTCCAAAGATACATTTTCATATGACTGACTACCTCAGCAGAAAAATAAATATGCTACCAAGCGATGTGGCTGTGGGGTTGAATAAACCGATGTTCAGTTCTCCATTATTTATTGAATATAGTGCTGAGCCATCCAGTGGTGGAGTGAAGAACATTGATAATGTAGAAGATCAAATGGAGACCGATCTAACTCTGCAAAGGAACATTAACAAAACAGTTATGGCCTGTGTAGATGATTTAGAGCCAACTGTTCCAACAGTGTCAGAAGATTTAGTACTTTATGAAGAAGCTAACATGCACGAGATAGATAGCTTGGCCAAATCTTACCTAGAGAAAAGTAAAGAAAGCATTTTGTCACCTGTGCTGGATGAGAATGTTAGCGGTGGTTGTAAAGTTGCAAATTCTACAATCCTTGAAGATGAAAGAATTGTGGCAGATTCGGAGGAAGAATTGTTAGTAGATCCAGGCAACCGCAATGGTGAAAGTGAAAACATGTCCCACCCACAAACTGCTCTCTCTGATAAGCTGCCTGGAATCGACTCCAGAAGATTAGAAGAGGAGGACTATGAAGATACAATACCTTTGAGAAAAAGGCTTATAAGAATACTTGAGACCCGAGTCAAACTTCCAGTTACTGATTTCTCTGTTGCTGAAAAGCAATTGTTGCAGCCATTTGAAAAGGTGTCCAAGGATTTAGTACTCTATGAAAGAACTAACATGCATGAGAAAACCAAAGAAAGCATAATCTCACCAGCTTTGGATGAGAGTGTTAGCGGTAGTCGTAAAGTTACAAACGCTACAATTCTTGAAGATGTAAGAACTGTGGGAAATCATGAGGAAGAACTGTTCATAGATCCTGGCAACCCTAATGGAGAGACTGAGAATCTATCACAGCCATTTGCTGCTGTTTCTGATAAGCAGCCTGGAACCGGCTCCAGAAGCTTAAAAGAGCAGGATTTTGAAGATACGATACCATTGCATAAAGAGCGACATGAGAACCAAACCATTTGTGTCGCCACTGATCTCTCCTCTCCTAAGGTGTCCAATGATTTAGTACTCTATGAAGGAGCTGACATGCATGAGATAGATATCCAGGGAAACTCTTACCAAGAGAAAATCAAAGAAAGCATAATGCCAGCTTTGGATGAGAGTGTTAGTGGTAACTGTAAAGGTACAAATGCTACAATGCTTGAAGATAAACGAATTGTGGGAGATGCAGAGGAAGGATTGTTAGTAAATCCTGGCAACCCTATTGGAGAAACAGAAATTCTATACCAGCCATATACTAATGTTTCTGATAAGCTGGCTGGAATTGACTTCAGAAGATTGAATGAGGATGATTATGAAGATACAACACCTGTGAATAAAAGTCTTATGACAAGAAATGAGACCCAAACCACCTGTCCTGCTACTGAATTCTATGTTCCTCAAAAGCAGTTGTTGAAGTCCTCTGCAACAATGAAGGCCATGGACAAGGATAAAATATCTTCAAGATTGCAATCTCTATATAGTTGTAGTCTAGAGCAAGATCAGACTGTTGCCACTCCCAAAGTGAACCTCCAACATAAAATGGATCAGAAGACAAATAGCATGGTACACAAATCAAAGCAGAATTGTGGTGACATGCATATTAATGAAAGAAAGAGACGATACAAATCTATTTCCTCTAAGGATCAGCCAGAGAAAAAAGAACTTCCAAATTTTGATTCTTATATGGTAGAAGAAGAAGAAGGTTCAGGTGGTTATGGCACTGTTTACAGGGCGAGGAGTAAGAATGACAGGACAAGGGTTGCCATTAAGTGTCCACATGCTAATGCTCATAAGAGCCATGTTAACAATGAGTTGAGAATGCTGGAAAGGTTTGGGGGGAAGAACTTTGTGATCAAATATGAAGGCTGTATCAAGAATGGAAATTCAAGTTGCTTTGTATTACAGCATGTTGAGCATGATAGGCCTGAGGTTTTAAAGAAAGAGATAGATGTTTGTGAGCTCCGGTGGTATGGATATTGCTTGTTTAGAGCACTTGCAAGCCTTCATAAGCAGGGAATCATACATAGAGATGTTAAACCCGGCAATTTCCTTTTCTCTCGTAAGGCCAACAAGGGTTACCTTATTGATTTCAATCTATCCATGGACTTGCATCAGAAGTATGGAAATAATAGTAAGTCAAGAGGGGGATTTGATGTAAGCAGCAATGATGAGAAGCTTATGACTGCAAAAAATTTACCTCCAATCACACTTAGGAAGTTGCCAAGTGGTAAATCTTTACTAACAGCCAATGGAGAGACCTCAAAAGGTTTGAAATCCACTTTTGATATGAAGGATCTAAAAAAGATGGCTTTCAGCCAAATGAAGGCCAATAATGATTCGGGTAGTAGGAAGGTAATCAAAAGTCAGGGTGCAGATGGCTCAGGCATAACGTCAGTAAAGGATGTTACAAGTACCAGAACTCCTTCGGCAGAAAGGATGAGAGAACCTTTGCCATGCCAAGGAAGGAAAGAACTGATCAGCCTAGTACAGGAAGCAAGGCAGAGTCCAAATCATGAGGCATCAAGTATTTCAGCTCTTATGAGAAAGAGGGTTCCTGCCCCTCCCGTAAATGTTGATGGCAAGCTTTTCCATGTCACTCCGATGCCTATGCACTCAACTACCAGTGGTATTGGTGGTCCATGCTTGATAAGAAGCAGAGGTCTTGGAAAACATAAAGAAGGCCCTTGTGCTGGAACAAAAGGCTTCAGGGCTCCAGAGGTTCTCTTCAGATCTCCACATCAAGGCCCCAAAATTGATATCTGGTCTGCCGGAGTCACGCTACTCTACTTGTTGATTGGCAGAACACCCTTCCTTGGTGAACCTGAACTGAACATCAAGGATATAGCAAAGCTGAGAGGCAGTGAAGATTTATGGGAAGTTGCCAAGCTACACGACCGTGAATCCTCATTTCCAGTGGAGCTCTATAGTACAGATTGTTTGCCCTCTATTAAACTGAAGGAGTGGTGTAGAATGAACACAAAGAGACCGGACTTCTTTGAGCAAATCCCAAGACCCCTTTTCGATCTTGTGGATAAATGCTTGATGGTGAACCCTAGGGTTAGATTGAGTGCAGAGGAAGCGCTTAAACATGAGTTTTTCGCTCCATGCCATGAAGAACTGAGAAAGCACAGGCTTCAAAGGCAGGTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006950 GO:0006974 GO:0007049 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0008360 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010389 GO:0010556 GO:0010557 GO:0010564 GO:0010571 GO:0010604 GO:0010948 GO:0010971 GO:0015630 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0033262 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045171 GO:0045740 GO:0045786 GO:0045787 GO:0045931 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070316 GO:0070317 GO:0071704 GO:0072686 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0098657 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1903047 GO:2000105 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 map04110 map04111 map04113
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1243

Amino Acids

138.48

Weight (kDa)

6.74

Isoelectric Point (pI)

49.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 755 - 900 6.6e-23 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 758 - 903 1.7e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 1090 - 1226 4.2e-16 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1148
Acc36I ACCTGC 1 cut(s) 3712
AccB1I GGYRCC 2 cut(s) 192, 398
AccB7I CCANNNNNTGG 1 cut(s) 686
AccBSI CCGCTC 2 cut(s) 319, 371
AccII CGCG 3 cut(s) 377, 444, 529
AclWI GGATC 6 cut(s) 98, 1004, 1397, 2130, 2229, 2880
AcoI YGGCCR 3 cut(s) 220, 474, 864
AcsI RAATTY 6 cut(s) 949, 1815, 1964, 2248, 2461, 2770
AcuI CTGAAG 4 cut(s) 1852, 3268, 3289, 3554
AcvI CACGTG 1 cut(s) 42
AcyI GRCGYC 2 cut(s) 292, 399
AfaI GTAC 8 cut(s) 825, 1248, 1605, 1723, 2145, 2992, 3066, 3508
AfeI AGCGCT 1 cut(s) 3660
AflIII ACRYGT 3 cut(s) 39, 41, 1041
AjnI CCWGG 6 cut(s) 1012, 1078, 1405, 1471, 1641, 1789
AjuI GAANNNNNNNTTGG 2 cut(s) 2239, 2271
AleI CACNNNNGTG 1 cut(s) 3474
AloI GAACNNNNNNTCC 2 cut(s) 1379, 1411
Alw21I GWGCWC 5 cut(s) 246, 415, 2541, 2572, 3501
Alw26I GTCTC 8 cut(s) 357, 722, 1154, 1418, 1931, 2188, 2828, 3556
Alw44I GTGCAC 1 cut(s) 411
AlwI GGATC 6 cut(s) 98, 1004, 1397, 2130, 2229, 2880
AlwNI CAGNNNCTG 1 cut(s) 1184
Ama87I CYCGRG 1 cut(s) 1164
Aor51HI AGCGCT 1 cut(s) 3660
ApaLI GTGCAC 1 cut(s) 411
ApeKI GCWGC 5 cut(s) 1075, 1216, 1451, 1468, 2739
ApoI RAATTY 6 cut(s) 949, 1815, 1964, 2248, 2461, 2770
ArsI GACNNNNNNTTYG 2 cut(s) 1936, 1968
AseI ATTAAT 1 cut(s) 2181
Asp700I GAANNNNTTC 1 cut(s) 3001
AspA2I CCTAGG 1 cut(s) 3632
AspLEI GCGC 4 cut(s) 401, 444, 529, 3661
AspS9I GGNCC 4 cut(s) 286, 3215, 3260, 3323
AsuC2I CCSGG 1 cut(s) 2619
AsuHPI GGTGA 7 cut(s) 148, 897, 1040, 1290, 2180, 3407, 3637
AvaI CYCGRG 1 cut(s) 1164
AvaII GGWCC 2 cut(s) 286, 3215
AvrII CCTAGG 1 cut(s) 3632
AxyI CCTNAGG 2 cut(s) 1584, 2505
BaeGI GKGCMC 2 cut(s) 415, 471
BalI TGGCCA 1 cut(s) 866
BanI GGYRCC 2 cut(s) 192, 398
BanII GRGCYC 4 cut(s) 246, 2541, 3292, 3501
BarI GAAGNNNNNNTAC 6 cut(s) 807, 839, 1110, 1142, 1503, 1535
BauI CACGAG 1 cut(s) 848
BbrPI CACGTG 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 2135
Bbv12I GWGCWC 5 cut(s) 246, 415, 2541, 2572, 3501
BbvCI CCTCAGC 1 cut(s) 584
BbvI GCAGC 5 cut(s) 1062, 1228, 1438, 1480, 2751
BccI CCATC 6 cut(s) 176, 688, 2878, 2948, 3149, 3616
BceAI ACGGC 2 cut(s) 276, 334
BcgI CGANNNNNNTGC 6 cut(s) 279, 313, 1507, 1541, 3169, 3203
BciT130I CCWGG 6 cut(s) 1014, 1080, 1407, 1473, 1643, 1791
BclI TGATCA 2 cut(s) 2430, 3054
BcnI CCSGG 1 cut(s) 2619
BcoDI GTCTC 8 cut(s) 357, 722, 1154, 1418, 1931, 2188, 2828, 3556
BfaI CTAG 4 cut(s) 878, 2069, 3062, 3633
BfmI CTRYAG 1 cut(s) 3502
BfoI RGCGCY 2 cut(s) 402, 3662
BfuAI ACCTGC 1 cut(s) 3712
BglII AGATCT 1 cut(s) 3308
BlnI CCTAGG 1 cut(s) 3632
BlpI GCTNAGC 1 cut(s) 675
BmcAI AGTACT 3 cut(s) 825, 1248, 1605
Bme1390I CCNGG 7 cut(s) 1014, 1080, 1407, 1473, 1643, 1791, 2619
Bme18I GGWCC 2 cut(s) 286, 3215
BmeT110I CYCGRG 1 cut(s) 1164
BmgT120I GGNCC 4 cut(s) 286, 3215, 3260, 3323
BmiI GGNNCC 7 cut(s) 194, 400, 1477, 1483, 3132, 3291, 3325
BmrFI CCNGG 7 cut(s) 1014, 1080, 1407, 1473, 1643, 1791, 2619
BmsI GCATC 5 cut(s) 245, 1753, 2698, 3107, 3171
BoxI GACNNNNGTC 1 cut(s) 1166
BpiI GAAGAC 1 cut(s) 2135
BpmI CTGGAG 3 cut(s) 1075, 1468, 3276
Bpu10I CCTNAGC 2 cut(s) 584, 2959
Bpu1102I GCTNAGC 1 cut(s) 675
BpuEI CTTGAG 1 cut(s) 1178
BpuMI CCSGG 1 cut(s) 2619
BsaAI YACGTR 1 cut(s) 42
BsaBI GATNNNNATC 5 cut(s) 108, 1637, 2047, 2203, 2670
BsaHI GRCGYC 2 cut(s) 292, 399
BsaI GGTCTC 5 cut(s) 722, 1154, 1931, 2828, 3556
BsaJI CCNNGG 9 cut(s) 126, 471, 1236, 1642, 2016, 2679, 3037, 3391, 3632
BsaWI WCCGGW 2 cut(s) 2541, 3565
BsaXI ACNNNNNCTCC 2 cut(s) 2983, 3013
Bse118I RCCGGY 2 cut(s) 404, 1478
Bse1I ACTGG 4 cut(s) 684, 1178, 3201, 3491
Bse21I CCTNAGG 2 cut(s) 1584, 2505
Bse3DI GCAATG 4 cut(s) 51, 1030, 1523, 2749
Bse8I GATNNNNATC 5 cut(s) 108, 1637, 2047, 2203, 2670
BseBI CCWGG 6 cut(s) 1014, 1080, 1407, 1473, 1643, 1791
BseDI CCNNGG 9 cut(s) 126, 471, 1236, 1642, 2016, 2679, 3037, 3391, 3632
BseGI GGATG 7 cut(s) 680, 922, 1315, 1699, 1891, 2986, 3021
BseJI GATNNNNATC 5 cut(s) 108, 1637, 2047, 2203, 2670
BseMI GCAATG 4 cut(s) 51, 1030, 1523, 2749
BseNI ACTGG 4 cut(s) 684, 1178, 3201, 3491
BseRI GAGGAG 6 cut(s) 188, 197, 540, 1121, 1567, 2329
BseSI GKGCMC 2 cut(s) 415, 471
BseX3I CGGCCG 1 cut(s) 220
BseXI GCAGC 5 cut(s) 1062, 1228, 1438, 1480, 2751
BseYI CCCAGC 1 cut(s) 268
BsgI GTGCAG 2 cut(s) 2970, 3669
Bsh1236I CGCG 3 cut(s) 377, 444, 529
Bsh1285I CGRYCG 2 cut(s) 223, 3475
BshNI GGYRCC 2 cut(s) 192, 398
BsiEI CGRYCG 2 cut(s) 223, 3475
BsiHKAI GWGCWC 5 cut(s) 246, 415, 2541, 2572, 3501
BsiHKCI CYCGRG 1 cut(s) 1164
BsiSI CCGG 7 cut(s) 396, 405, 1479, 2542, 2619, 3348, 3566
BslFI GGGAC 2 cut(s) 30, 1030
BsmAI GTCTC 8 cut(s) 357, 722, 1154, 1418, 1931, 2188, 2828, 3556
BsmBI CGTCTC 2 cut(s) 357, 2188
BsmFI GGGAC 2 cut(s) 30, 1030
BsmI GAATGC 1 cut(s) 2403
Bso31I GGTCTC 5 cut(s) 722, 1154, 1931, 2828, 3556
BsoBI CYCGRG 1 cut(s) 1164
Bsp1286I GDGCHC 7 cut(s) 246, 415, 471, 2541, 2572, 3292, 3501
Bsp1720I GCTNAGC 1 cut(s) 675
Bsp19I CCATGG 3 cut(s) 126, 2016, 2679
BspFNI CGCG 3 cut(s) 377, 444, 529
BspHI TCATGA 2 cut(s) 1381, 3091
BspLI GGNNCC 7 cut(s) 194, 400, 1477, 1483, 3132, 3291, 3325
BspMI ACCTGC 1 cut(s) 3712
BspPI GGATC 6 cut(s) 98, 1004, 1397, 2130, 2229, 2880
BspT107I GGYRCC 2 cut(s) 192, 398
BspTNI GGTCTC 5 cut(s) 722, 1154, 1931, 2828, 3556
BsrBI CCGCTC 2 cut(s) 319, 371
BsrDI GCAATG 4 cut(s) 51, 1030, 1523, 2749
BsrFI RCCGGY 2 cut(s) 404, 1478
BsrI ACTGG 4 cut(s) 684, 1178, 3201, 3491
BssAI RCCGGY 2 cut(s) 404, 1478
BssECI CCNNGG 9 cut(s) 126, 471, 1236, 1642, 2016, 2679, 3037, 3391, 3632
BssNI GRCGYC 2 cut(s) 292, 399
BssSI CACGAG 1 cut(s) 848
BssT1I CCWWGG 7 cut(s) 126, 1236, 2016, 2679, 3037, 3391, 3632
Bst2BI CACGAG 1 cut(s) 848
Bst2UI CCWGG 6 cut(s) 1014, 1080, 1407, 1473, 1643, 1791
Bst4CI ACNGT 9 cut(s) 766, 799, 808, 1372, 1395, 1715, 2086, 2302, 3476
Bst6I CTCTTC 4 cut(s) 428, 510, 1098, 3308
BstACI GRCGYC 2 cut(s) 292, 399
BstAPI GCANNNNNTGC 1 cut(s) 1213
BstBAI YACGTR 1 cut(s) 42
BstC8I GCNNGC 6 cut(s) 246, 406, 1686, 1854, 2578, 3161
BstDSI CCRYGG 3 cut(s) 126, 2016, 2679
BstEII GGTNACC 1 cut(s) 2654
BstENI CCTNNNNNAGG 1 cut(s) 3065
BstF5I GGATG 7 cut(s) 680, 922, 1315, 1699, 1891, 2986, 3021
BstFNI CGCG 3 cut(s) 377, 444, 529
BstH2I RGCGCY 2 cut(s) 402, 3662
BstHHI GCGC 4 cut(s) 401, 444, 529, 3661
BstMAI GTCTC 8 cut(s) 357, 722, 1154, 1418, 1931, 2188, 2828, 3556
BstMCI CGRYCG 2 cut(s) 223, 3475
BstNI CCWGG 6 cut(s) 1014, 1080, 1407, 1473, 1643, 1791
BstNSI RCATGY 7 cut(s) 847, 1045, 1270, 1627, 2176, 2360, 2489
BstPAI GACNNNNGTC 1 cut(s) 1166
BstPI GGTNACC 1 cut(s) 2654
BstSCI CCNGG 7 cut(s) 1012, 1078, 1405, 1471, 1641, 1789, 2617
BstSFI CTRYAG 1 cut(s) 3502
BstSLI GKGCMC 2 cut(s) 415, 471
BstUI CGCG 3 cut(s) 377, 444, 529
BstV1I GCAGC 5 cut(s) 1062, 1228, 1438, 1480, 2751
BstV2I GAAGAC 1 cut(s) 2135
BstX2I RGATCY 4 cut(s) 1009, 1402, 2872, 3308
BstXI CCANNNNNNTGG 1 cut(s) 127
BstYI RGATCY 4 cut(s) 1009, 1402, 2872, 3308
BstZI CGGCCG 1 cut(s) 220
Bsu36I CCTNAGG 2 cut(s) 1584, 2505
BtgI CCRYGG 3 cut(s) 126, 2016, 2679
BtgZI GCGATG 1 cut(s) 627
BtsCI GGATG 7 cut(s) 680, 922, 1315, 1699, 1891, 2986, 3021
BtsI GCAGTG 1 cut(s) 3445
BtsIMutI CAGTG 8 cut(s) 413, 691, 813, 1566, 2298, 3208, 3445, 3498
BveI ACCTGC 1 cut(s) 3712
Cac8I GCNNGC 6 cut(s) 246, 406, 1686, 1854, 2578, 3161
CaiI CAGNNNCTG 1 cut(s) 1184
CciI TCATGA 2 cut(s) 1381, 3091
CfoI GCGC 4 cut(s) 401, 444, 529, 3661
Cfr10I RCCGGY 2 cut(s) 404, 1478
Cfr13I GGNCC 4 cut(s) 286, 3215, 3260, 3323
CpoI CGGWCCG 1 cut(s) 286
CseI GACGC 2 cut(s) 300, 433
Csp6I GTAC 8 cut(s) 824, 1247, 1604, 1722, 2144, 2991, 3065, 3507
CspCI CAANNNNNGTGG 2 cut(s) 597, 632
CspI CGGWCCG 1 cut(s) 286
CviQI GTAC 8 cut(s) 824, 1247, 1604, 1722, 2144, 2991, 3065, 3507
DinI GGCGCC 1 cut(s) 400
DraI TTTAAA 1 cut(s) 2514
EaeI YGGCCR 3 cut(s) 220, 474, 864
EagI CGGCCG 1 cut(s) 220
Eam1104I CTCTTC 4 cut(s) 428, 510, 1098, 3308
EarI CTCTTC 4 cut(s) 428, 510, 1098, 3308
Ecl136II GAGCTC 3 cut(s) 244, 2539, 3499
EclXI CGGCCG 1 cut(s) 220
Eco130I CCWWGG 7 cut(s) 126, 1236, 2016, 2679, 3037, 3391, 3632
Eco147I AGGCCT 1 cut(s) 2503
Eco24I GRGCYC 4 cut(s) 246, 2541, 3292, 3501
Eco31I GGTCTC 5 cut(s) 722, 1154, 1931, 2828, 3556
Eco32I GATATC 2 cut(s) 1639, 3337
Eco47I GGWCC 2 cut(s) 286, 3215
Eco47III AGCGCT 1 cut(s) 3660
Eco52I CGGCCG 1 cut(s) 220
Eco53kI GAGCTC 3 cut(s) 244, 2539, 3499
Eco57I CTGAAG 4 cut(s) 1852, 3268, 3289, 3554
Eco72I CACGTG 1 cut(s) 42
Eco81I CCTNAGG 2 cut(s) 1584, 2505
Eco88I CYCGRG 1 cut(s) 1164
Eco91I GGTNACC 1 cut(s) 2654
EcoICRI GAGCTC 3 cut(s) 244, 2539, 3499
EcoNI CCTNNNNNAGG 1 cut(s) 3065
EcoO109I RGGNCCY 2 cut(s) 3260, 3323
EcoO65I GGTNACC 1 cut(s) 2654
EcoRI GAATTC 1 cut(s) 1964
EcoRII CCWGG 6 cut(s) 1012, 1078, 1405, 1471, 1641, 1789
EcoRV GATATC 2 cut(s) 1639, 3337
EcoT14I CCWWGG 7 cut(s) 126, 1236, 2016, 2679, 3037, 3391, 3632
EcoT22I ATGCAT 3 cut(s) 1272, 1629, 2178
EcoT38I GRGCYC 4 cut(s) 246, 2541, 3292, 3501
EgeI GGCGCC 1 cut(s) 400
EheI GGCGCC 1 cut(s) 400
ErhI CCWWGG 7 cut(s) 126, 1236, 2016, 2679, 3037, 3391, 3632
Esp3I CGTCTC 2 cut(s) 357, 2188
FaqI GGGAC 2 cut(s) 30, 1030
FauI CCCGC 2 cut(s) 56, 240
FauNDI CATATG 1 cut(s) 570
FbaI TGATCA 2 cut(s) 2430, 3054
FokI GGATG 7 cut(s) 667, 929, 1322, 1706, 1898, 2993, 3028
FriOI GRGCYC 4 cut(s) 246, 2541, 3292, 3501
FspBI CTAG 4 cut(s) 878, 2069, 3062, 3633
GlaI GCGC 4 cut(s) 400, 443, 528, 3660
GsaI CCCAGC 1 cut(s) 272
GsuI CTGGAG 3 cut(s) 1075, 1468, 3276
HaeII RGCGCY 2 cut(s) 402, 3662
HapII CCGG 7 cut(s) 396, 405, 1479, 2542, 2619, 3348, 3566
HgaI GACGC 2 cut(s) 300, 433
HhaI GCGC 4 cut(s) 401, 444, 529, 3661
Hin1I GRCGYC 2 cut(s) 292, 399
Hin6I GCGC 4 cut(s) 399, 442, 527, 3659
HinP1I GCGC 4 cut(s) 399, 442, 527, 3659
HincII GTYRAC 1 cut(s) 2383
HindII GTYRAC 1 cut(s) 2383
HindIII AAGCTT 3 cut(s) 1489, 2753, 3161
HpaI GTTAAC 1 cut(s) 2383
HpaII CCGG 7 cut(s) 396, 405, 1479, 2542, 2619, 3348, 3566
HphI GGTGA 7 cut(s) 148, 897, 1040, 1290, 2180, 3407, 3637
Hpy166II GTNNAC 8 cut(s) 413, 2104, 2146, 2305, 2354, 2383, 3398, 3628
Hpy8I GTNNAC 8 cut(s) 413, 2104, 2146, 2305, 2354, 2383, 3398, 3628
Hpy99I CGWCG 4 cut(s) 294, 308, 335, 449
HpyCH4III ACNGT 9 cut(s) 766, 799, 808, 1372, 1395, 1715, 2086, 2302, 3476
HpyCH4IV ACGT 3 cut(s) 41, 342, 2969
HpySE526I ACGT 3 cut(s) 41, 342, 2969
Hsp92I GRCGYC 2 cut(s) 292, 399
HspAI GCGC 4 cut(s) 399, 442, 527, 3659
KasI GGCGCC 1 cut(s) 398
KroI GCCGGC 1 cut(s) 404
KroNI GCCGGC 1 cut(s) 406
Ksp22I TGATCA 2 cut(s) 2430, 3054
KspAI GTTAAC 1 cut(s) 2383
LmnI GCTCC 8 cut(s) 305, 524, 1487, 1616, 2544, 3295, 3496, 3685
Lsp1109I GCAGC 5 cut(s) 1062, 1228, 1438, 1480, 2751
LweI GCATC 5 cut(s) 245, 1753, 2698, 3107, 3171
MaeI CTAG 4 cut(s) 878, 2069, 3062, 3633
MaeII ACGT 3 cut(s) 41, 342, 2969
MbiI CCGCTC 2 cut(s) 319, 371
MfeI CAATTG 1 cut(s) 1208
MflI RGATCY 4 cut(s) 1009, 1402, 2872, 3308
MhlI GDGCHC 7 cut(s) 246, 415, 471, 2541, 2572, 3292, 3501
MlsI TGGCCA 1 cut(s) 866
MluNI TGGCCA 1 cut(s) 866
Mly113I GGCGCC 1 cut(s) 399
MlyI GAGTC 7 cut(s) 140, 170, 389, 1082, 1176, 3091, 3360
MmeI TCCRAC 6 cut(s) 198, 204, 313, 354, 827, 2134
Mox20I TGGCCA 1 cut(s) 866
Mph1103I ATGCAT 3 cut(s) 1272, 1629, 2178
MreI CGCCGGCG 1 cut(s) 404
MroNI GCCGGC 1 cut(s) 404
MroXI GAANNNNTTC 1 cut(s) 3001
MscI TGGCCA 1 cut(s) 866
MslI CAYNNNNRTG 6 cut(s) 125, 2181, 2361, 2607, 3179, 3474
Msp20I TGGCCA 1 cut(s) 866
MspI CCGG 7 cut(s) 396, 405, 1479, 2542, 2619, 3348, 3566
MspR9I CCNGG 7 cut(s) 1014, 1080, 1407, 1473, 1643, 1791, 2619
MunI CAATTG 1 cut(s) 1208
Mva1269I GAATGC 1 cut(s) 2403
MvaI CCWGG 6 cut(s) 1014, 1080, 1407, 1473, 1643, 1791
MvnI CGCG 3 cut(s) 377, 444, 529
NaeI GCCGGC 1 cut(s) 406
NarI GGCGCC 1 cut(s) 399
NciI CCSGG 1 cut(s) 2619
NcoI CCATGG 3 cut(s) 126, 2016, 2679
NdeI CATATG 1 cut(s) 570
NgoMIV GCCGGC 1 cut(s) 404
NlaIV GGNNCC 7 cut(s) 194, 400, 1477, 1483, 3132, 3291, 3325
NmeAIII GCCGAG 2 cut(s) 198, 452
NmuCI GTSAC 5 cut(s) 343, 903, 2168, 3172, 3352
NsiI ATGCAT 3 cut(s) 1272, 1629, 2178
NspI RCATGY 7 cut(s) 847, 1045, 1270, 1627, 2176, 2360, 2489
OliI CACNNNNGTG 1 cut(s) 3474
PagI TCATGA 2 cut(s) 1381, 3091
PceI AGGCCT 1 cut(s) 2503
PciI ACATGT 1 cut(s) 1041
PcsI WCGNNNNNNNCGW 2 cut(s) 339, 381
PctI GAATGC 1 cut(s) 2403
PdiI GCCGGC 1 cut(s) 406
PdmI GAANNNNTTC 1 cut(s) 3001
PfeI GAWTC 7 cut(s) 986, 1083, 1432, 2255, 2595, 2915, 3479
PflMI CCANNNNNTGG 1 cut(s) 686
PleI GAGTC 7 cut(s) 140, 170, 388, 1082, 1175, 3090, 3359
PluTI GGCGCC 1 cut(s) 402
PmaCI CACGTG 1 cut(s) 42
PmlI CACGTG 1 cut(s) 42
PpsI GAGTC 7 cut(s) 140, 170, 388, 1082, 1175, 3090, 3359
Ppu21I YACGTR 1 cut(s) 42
PscI ACATGT 1 cut(s) 1041
PshAI GACNNNNGTC 1 cut(s) 1166
PshBI ATTAAT 1 cut(s) 2181
PsiI TTATAA 1 cut(s) 1148
Psp124BI GAGCTC 3 cut(s) 246, 2541, 3501
Psp6I CCWGG 6 cut(s) 1012, 1078, 1405, 1471, 1641, 1789
PspCI CACGTG 1 cut(s) 42
PspEI GGTNACC 1 cut(s) 2654
PspFI CCCAGC 1 cut(s) 268
PspGI CCWGG 6 cut(s) 1012, 1078, 1405, 1471, 1641, 1789
PspN4I GGNNCC 7 cut(s) 194, 400, 1477, 1483, 3132, 3291, 3325
PspPI GGNCC 4 cut(s) 286, 3215, 3260, 3323
PstNI CAGNNNCTG 1 cut(s) 1184
PsuI RGATCY 4 cut(s) 1009, 1402, 2872, 3308
RsaI GTAC 8 cut(s) 825, 1248, 1605, 1723, 2145, 2992, 3066, 3508
RsaNI GTAC 8 cut(s) 824, 1247, 1604, 1722, 2144, 2991, 3065, 3507
RseI CAYNNNNRTG 6 cut(s) 125, 2181, 2361, 2607, 3179, 3474
Rsr2I CGGWCCG 1 cut(s) 286
RsrII CGGWCCG 1 cut(s) 286
SacI GAGCTC 3 cut(s) 246, 2541, 3501
Sau96I GGNCC 4 cut(s) 286, 3215, 3260, 3323
ScaI AGTACT 3 cut(s) 825, 1248, 1605
SchI GAGTC 7 cut(s) 140, 170, 389, 1082, 1176, 3091, 3360
ScrFI CCNGG 7 cut(s) 1014, 1080, 1407, 1473, 1643, 1791, 2619
SduI GDGCHC 7 cut(s) 246, 415, 471, 2541, 2572, 3292, 3501
SfaNI GCATC 5 cut(s) 245, 1753, 2698, 3107, 3171
SfcI CTRYAG 1 cut(s) 3502
SfoI GGCGCC 1 cut(s) 400
SgrAI CRCCGGYG 1 cut(s) 404
SinI GGWCC 2 cut(s) 286, 3215
SmiMI CAYNNNNRTG 6 cut(s) 125, 2181, 2361, 2607, 3179, 3474
SmlI CTYRAG 1 cut(s) 1157
SmoI CTYRAG 1 cut(s) 1157
SseBI AGGCCT 1 cut(s) 2503
SspDI GGCGCC 1 cut(s) 398
SspMI CTAG 4 cut(s) 878, 2069, 3062, 3633
SstI GAGCTC 3 cut(s) 246, 2541, 3501
StuI AGGCCT 1 cut(s) 2503
StyD4I CCNGG 7 cut(s) 1012, 1078, 1405, 1471, 1641, 1789, 2617
StyI CCWWGG 7 cut(s) 126, 1236, 2016, 2679, 3037, 3391, 3632
TaaI ACNGT 9 cut(s) 766, 799, 808, 1372, 1395, 1715, 2086, 2302, 3476
TaiI ACGT 3 cut(s) 44, 345, 2972
TaqI TCGA 3 cut(s) 144, 1086, 3600
TaqII GACCGA 2 cut(s) 274, 746
TatI WGTACW 5 cut(s) 823, 1246, 1603, 3064, 3506
TauI GCSGC 6 cut(s) 59, 225, 228, 251, 319, 404
TfiI GAWTC 7 cut(s) 986, 1083, 1432, 2255, 2595, 2915, 3479
TscAI CASTG 8 cut(s) 420, 691, 813, 1573, 2305, 3208, 3445, 3498
TseFI GTSAC 5 cut(s) 343, 903, 2168, 3172, 3352
TseI GCWGC 5 cut(s) 1075, 1216, 1451, 1468, 2739
Tsp45I GTSAC 5 cut(s) 343, 903, 2168, 3172, 3352
TspGWI ACGGA 3 cut(s) 292, 373, 496
TspRI CASTG 8 cut(s) 420, 691, 813, 1573, 2305, 3208, 3445, 3498
Van91I CCANNNNNTGG 1 cut(s) 686
VneI GTGCAC 1 cut(s) 411
VpaK11BI GGWCC 2 cut(s) 286, 3215
VspI ATTAAT 1 cut(s) 2181
XagI CCTNNNNNAGG 1 cut(s) 3065
XapI RAATTY 6 cut(s) 949, 1815, 1964, 2248, 2461, 2770
XbaI TCTAGA 1 cut(s) 2068
XceI RCATGY 7 cut(s) 847, 1045, 1270, 1627, 2176, 2360, 2489
XmaJI CCTAGG 1 cut(s) 3632
XmnI GAANNNNTTC 1 cut(s) 3001
XspI CTAG 4 cut(s) 878, 2069, 3062, 3633
ZrmI AGTACT 3 cut(s) 825, 1248, 1605
Zsp2I ATGCAT 3 cut(s) 1272, 1629, 2178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.