RLG00000003391

serine threonine-protein kinase cdc7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002088
Physical Location & Seq
Reverse (-)
47987485 .. 47993565
6081 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003391

Sequence Viewer

Length: 3291 bp
ATGGCAACTAACCCACTCGACTCAGCCCTCACCGCCTCAACCATCTCCGACTCCGACAAGGCTTGGCACCTCCTCGCCCTCCTCTTATCTCTCGGCCGCCGGACCCCGCCCTCCGAGCTCGCCGCCCGATGCACGGCGTTCCCGGCAACTACTCATTTGGTCCGACGTCTTTGCTCCGTCGCCAATTCGCCGCTCTCCTTGTCCGACGATCTATACGTCACGCCGTCTCTACTCGCCGTAACCGCTCTCGCGGAGTCCGTTTCATGTTCCGGCGCCGCCGGCGGTGCACTGAGAACCTACTCAGGGAAGAGGAAGCGCGTCGTTTTGGATTGTGGATTTCTGCCCTCGGCCAAACGGAGATTGATTGTGGATTTTGAAAATGTAAATGAAGAGAAAGGAGCGTGGAAATCAGTCGTAATTTCTCCTCCTAAGATACATTTTCATATGACTGACTACCTCAGCAGAAGAATAAATATGCTACCAAGTGACGTGGCTGTGGGGTTGAATAAACCGATGTTCAGTACTCCATTATTTATTGAATATAGTGCTGGGCCATTCCATGGTGGAGTGAAGAACATTGACAATGTAGAAGATCAAATGGAGACCGACCTAACTCTGCAAAGGAACATTACCAAAACAGTTATGGCCTGTGTAGATGATTTAGAGCCAACTAATCCAACAGTGTCAGAAGATTTAGTACTTTATGAAGAAGCTAACATGCACAAGATAGATAGCTTGGCCAAATCTTACCTAGAGACAAGTAAACAAAGCATTTTGTCACCTGTGCTGGATGAGAATGTTAGCGGTAGTTGTAAAGTTGCAAATTCTACAATCCTTGAAGATGAAAGAATTGTGGCAGATTCGGAGGAAGAATTGTTAGTAGATCCAGGCAACCGCAATGGTGAAAGAGAAAACATGTCCCTCCCACAAACTGCTGTTTCTGATAAGCTGCCTGGAATCAACTCCAGAAGATTAGAAGAGGAGGACTATGAAGATACAATACCTTTGAGAAAAAGGCTTATAAGAATACTTGAGACCCGAGTCAAACTTCCATTTAGTGATTTCTCTGTTGCTGAAAAGCAATTCTTGCAGCCATTTGAAAAGGCGTCCAAGGATTTAGTACTGTATGAAAGAGCTAACATGCATGAGAAAACCAAAGAAAGCATAATCTCACCAGCTTTGGATGAGAGTGTTAGTGGTAGTCGTAAAGTTAGAAACGCTACAATTCTTGAAGATATAAGAACTGTGGGAAATCATGAGGAAGAACTGTTCATAGATCCTGGCAACCCTAATGGAGAAACTGAGAATCTATCACAGCCATTTGCTGCTGTTTCTGATAAGCAGCCTGGAACCGGCTCCAGAAGATTAAAAGAGCAGGATTTTGAGGATTCGATACCATTGCATAAAAAGCGACATGAGAACCAAACTATTTGTGTGGCTACTTATTTCTCCTCTCCTAAGGTGTCCAATGATTTAGTACTCTATGAAGGAGCTGACATGCATGAGATAGATAACCAGGGAAACTCTTACCAAGAGAAAATCAAAGAAAGCATAATGCCAGCTTTTGATGAGAGTGTTAGTGGTAACTGTAAAGGTACAAATGCTACAATCCTTGAAGATAAACGAATTGTGGGAGATGCAGAGGAAGGATTGTTAGTAAATCCTGGCAACCCTATTGGAGAAACAGAAATTCTATACCAGCCATATAATAATGTTTCTGATAAGCTGGCTGGAATTGACTTCAGAAGATTGAATGAGGATGATTATGAAGATACAACACCTGTGAATAAAAGTCTTATGACAAGAAATGAGACCCAAACCACCTGTCCTGCTACTGAATTCTATGTTCCTCAAAATCAGTTGTTGAAGTCCTCTGCAACAATGAAGGCCATGAACAAGGATAAAATCTCTTCAAGATTGCAATCTCTATATGGTTGTAGTCTAGAGCAAGATCAGACTGTTGGCACTCCCAAAGTGAACCTCCACCATAAAATGGATCAGAAGACAAATAGCATGGTACACAAATCAAAGCAGAATTGTGGTGACATGCATATTAATGAAAGAAAGAGACGATACAAATCTATTTCCTCTAAGGATCAGCCAGAGGAAAAAGAACTTCCAAATTTTGATTCTTATATGGTAGAAGAAGAAGAAGGTTCAGGTGGTTATGGCACTGTTTACAGGGCGAGGAGTAAGAATGACAGGACAAGGGTTGCCATTAAGTGTCCACATGCTAATGCTCATAAGAGCCATGTTAACAATGAGTTGAGAATGCTGGAAAGGTTTGGGGGGAAGAACTTTGTGATCAAATATGAAGGCTGTATCAAGAATGGAAATTCAAGTTGCTTTGTATTACAGCATGTTGAGCATGATAGGCCTGAGGTTTTAAAGAAAGAGATAGATGTTTGTGAGCTCCGGTGGTATGGATATTGCTTGTTTAGAGCACTTGCAAGCCTTCATAAGCAGGGAATAGTACATAGAGATGTTAAACCTGGCAATTTCCTTTTCTCTCGTAAGGCCAACAAGGGTTACCTTATTGATTTCAATCTATCCATGGACTTGCATCAGAAGTATGGAAATAATAGTAAGTCAAGAGGGGGATTTGATGTAAGCAGCAATGATGAAAAGCTTATGACTGCAAAAAATTTACCTCCAATCACACTTAGGAAGTTGCCAAGTGGTAAATCTTTACTAACAGCCAATGGAGAGACCTCAAAAGGTTCGAAATCCACTTTTGATATGAAGGATCTAAAAAAGATGGCTTTCAGCCAAATGAAGGCCAATAATGATTCGGGTAGTAGGAAGGTAATCAAAAGTCAGGGTGCAGATGGCTCAGGCATAACGTCAGTCAAGGATGTTCTCTTCAGATCTCCACATCAAGGCCCCAAAATTGATATCTGGTCGGCCGGAGTCACGCTACTCTACTTGTTGATTGGCAGAACACCCTTCCTTGGTGAACCTGAACTGAACATCAAGGATATAGCAAAGCTGAGAGGCAGTGAAGATTTATGGGAAGTTGCCAAGCTACACGACCGTGAATCCTCATTTCCAGTGGAGCTCTATAGTACAGATTGTTTGCCCTCTATTAAACTAAAGGAGTGGTGTAGAATGAACACAAAGAGACCGGACTTCTTTGAGCAAATCCCAAGACCCCTTTTCGATCTTGTGGATAAATGCTTGATGGTGAACCCTAGGGTTAGATTGAGTGCAGAGGAAGCGCTTAAACATGAGTTTTTCGCTCCATGCCATGAAGAACTGAGAAAGCACAGGCTTCAGAGGCAGGTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006950 GO:0006974 GO:0007049 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0008360 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010389 GO:0010556 GO:0010557 GO:0010564 GO:0010571 GO:0010604 GO:0010948 GO:0010971 GO:0015630 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0033262 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045171 GO:0045740 GO:0045786 GO:0045787 GO:0045931 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070316 GO:0070317 GO:0071704 GO:0072686 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0098657 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1903047 GO:2000105 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 map04110 map04111 map04113
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1097

Amino Acids

123.06

Weight (kDa)

6.44

Isoelectric Point (pI)

50.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 713 - 858 5.9e-23 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 716 - 861 1.4e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 956 - 1079 8.8e-11 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1022
AatII GACGTC 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 3271
AccB1I GGYRCC 2 cut(s) 66, 272
AccB7I CCANNNNNTGG 2 cut(s) 560, 1993
AccBSI CCGCTC 2 cut(s) 193, 245
AccII CGCG 2 cut(s) 251, 318
AclWI GGATC 5 cut(s) 878, 1271, 2004, 2103, 2754
AcoI YGGCCR 4 cut(s) 94, 348, 738, 2904
AcsI RAATTY 6 cut(s) 823, 1689, 1838, 2122, 2335, 2644
AcuI CTGAAG 3 cut(s) 1726, 2848, 3257
AcyI GRCGYC 3 cut(s) 166, 273, 1106
AfaI GTAC 8 cut(s) 523, 699, 1122, 1479, 1597, 2019, 2475, 3067
AfeI AGCGCT 1 cut(s) 3219
AfiI CCNNNNNNNGG 8 cut(s) 133, 303, 560, 1352, 1993, 2776, 2879, 2951
AflIII ACRYGT 1 cut(s) 915
AjiI CACGTC 1 cut(s) 490
AjnI CCWGG 7 cut(s) 886, 952, 1279, 1345, 1515, 1663, 2491
AjuI GAANNNNNNNTTGG 2 cut(s) 2113, 2145
AleI CACNNNNGTG 1 cut(s) 3033
AloI GAACNNNNNNTCC 2 cut(s) 1253, 1285
Alw21I GWGCWC 5 cut(s) 120, 289, 2415, 2446, 3060
Alw26I GTCTC 8 cut(s) 231, 596, 749, 1028, 1805, 2062, 2702, 3115
Alw44I GTGCAC 1 cut(s) 285
AlwI GGATC 5 cut(s) 878, 1271, 2004, 2103, 2754
Ama87I CYCGRG 1 cut(s) 1038
Aor51HI AGCGCT 1 cut(s) 3219
ApaLI GTGCAC 1 cut(s) 285
ApeKI GCWGC 5 cut(s) 949, 1090, 1325, 1342, 2613
ApoI RAATTY 6 cut(s) 823, 1689, 1838, 2122, 2335, 2644
ArsI GACNNNNNNTTYG 2 cut(s) 1810, 1842
AseI ATTAAT 1 cut(s) 2055
AspA2I CCTAGG 1 cut(s) 3191
AspLEI GCGC 3 cut(s) 275, 318, 3220
AspS9I GGNCC 4 cut(s) 102, 160, 551, 2882
AsuC2I CCSGG 1 cut(s) 143
AsuHPI GGTGA 7 cut(s) 22, 771, 914, 1164, 2054, 2966, 3196
AsuII TTCGAA 1 cut(s) 2723
AvaI CYCGRG 1 cut(s) 1038
AvaII GGWCC 2 cut(s) 102, 160
AvrII CCTAGG 1 cut(s) 3191
AxyI CCTNAGG 2 cut(s) 1458, 2379
BaeGI GKGCMC 1 cut(s) 289
BalI TGGCCA 1 cut(s) 740
BanI GGYRCC 2 cut(s) 66, 272
BanII GRGCYC 3 cut(s) 120, 2415, 3060
BarI GAAGNNNNNNTAC 4 cut(s) 681, 713, 984, 1016
BbsI GAAGAC 1 cut(s) 2009
Bbv12I GWGCWC 5 cut(s) 120, 289, 2415, 2446, 3060
BbvCI CCTCAGC 1 cut(s) 458
BbvI GCAGC 5 cut(s) 936, 1102, 1312, 1354, 2625
BccI CCATC 4 cut(s) 50, 2752, 2822, 3175
BceAI ACGGC 3 cut(s) 150, 208, 221
BcgI CGANNNNNNTGC 4 cut(s) 153, 187, 1381, 1415
BciT130I CCWGG 7 cut(s) 888, 954, 1281, 1347, 1517, 1665, 2493
BclI TGATCA 1 cut(s) 2304
BcnI CCSGG 1 cut(s) 143
BcoDI GTCTC 8 cut(s) 231, 596, 749, 1028, 1805, 2062, 2702, 3115
BfaI CTAG 3 cut(s) 752, 1943, 3192
BfmI CTRYAG 1 cut(s) 3061
BfoI RGCGCY 2 cut(s) 276, 3221
BfuAI ACCTGC 1 cut(s) 3271
BglII AGATCT 1 cut(s) 2867
BisI GCNGC 9 cut(s) 97, 123, 191, 276, 950, 1091, 1326, 1343, 2614
BlnI CCTAGG 1 cut(s) 3191
BlsI GCNGC 9 cut(s) 98, 124, 192, 277, 951, 1092, 1327, 1344, 2615
BmcAI AGTACT 4 cut(s) 523, 699, 1122, 1479
Bme1390I CCNGG 8 cut(s) 143, 888, 954, 1281, 1347, 1517, 1665, 2493
Bme18I GGWCC 2 cut(s) 102, 160
BmeT110I CYCGRG 1 cut(s) 1038
BmgBI CACGTC 1 cut(s) 490
BmgT120I GGNCC 4 cut(s) 102, 160, 551, 2882
BmiI GGNNCC 6 cut(s) 68, 104, 274, 1351, 1357, 2884
BmrFI CCNGG 8 cut(s) 143, 888, 954, 1281, 1347, 1517, 1665, 2493
BmsI GCATC 3 cut(s) 119, 1627, 2572
BoxI GACNNNNGTC 1 cut(s) 1040
BpiI GAAGAC 1 cut(s) 2009
BpmI CTGGAG 2 cut(s) 949, 1342
Bpu10I CCTNAGC 2 cut(s) 458, 2833
Bpu14I TTCGAA 1 cut(s) 2723
BpuEI CTTGAG 1 cut(s) 1052
BpuMI CCSGG 1 cut(s) 143
BsaBI GATNNNNATC 3 cut(s) 1921, 2077, 2544
BsaHI GRCGYC 3 cut(s) 166, 273, 1106
BsaI GGTCTC 5 cut(s) 596, 1028, 1805, 2702, 3115
BsaJI CCNNGG 7 cut(s) 345, 559, 1110, 1516, 2553, 2950, 3191
BsaWI WCCGGW 2 cut(s) 2415, 3124
Bsc4I CCNNNNNNNGG 8 cut(s) 133, 303, 560, 1352, 1993, 2776, 2879, 2951
Bse118I RCCGGY 2 cut(s) 278, 1352
Bse1I ACTGG 1 cut(s) 3050
Bse21I CCTNAGG 2 cut(s) 1458, 2379
Bse3DI GCAATG 3 cut(s) 904, 1397, 2623
Bse8I GATNNNNATC 3 cut(s) 1921, 2077, 2544
BseBI CCWGG 7 cut(s) 888, 954, 1281, 1347, 1517, 1665, 2493
BseDI CCNNGG 7 cut(s) 345, 559, 1110, 1516, 2553, 2950, 3191
BseGI GGATG 4 cut(s) 796, 1189, 1765, 2860
BseJI GATNNNNATC 3 cut(s) 1921, 2077, 2544
BseLI CCNNNNNNNGG 8 cut(s) 133, 303, 560, 1352, 1993, 2776, 2879, 2951
BseMI GCAATG 3 cut(s) 904, 1397, 2623
BseMII CTCAG 9 cut(s) 36, 281, 315, 472, 1293, 2370, 2847, 2981, 3248
BseNI ACTGG 1 cut(s) 3050
BseRI GAGGAG 6 cut(s) 62, 71, 414, 995, 1441, 2203
BseSI GKGCMC 1 cut(s) 289
BseX3I CGGCCG 2 cut(s) 94, 2904
BseXI GCAGC 5 cut(s) 936, 1102, 1312, 1354, 2625
BseYI CCCAGC 1 cut(s) 548
BsgI GTGCAG 2 cut(s) 2844, 3228
Bsh1236I CGCG 2 cut(s) 251, 318
Bsh1285I CGRYCG 3 cut(s) 97, 2907, 3034
BshNI GGYRCC 2 cut(s) 66, 272
BsiEI CGRYCG 3 cut(s) 97, 2907, 3034
BsiHKAI GWGCWC 5 cut(s) 120, 289, 2415, 2446, 3060
BsiHKCI CYCGRG 1 cut(s) 1038
BsiSI CCGG 8 cut(s) 100, 143, 270, 279, 1353, 2416, 2907, 3125
BslFI GGGAC 1 cut(s) 904
BslI CCNNNNNNNGG 8 cut(s) 133, 303, 560, 1352, 1993, 2776, 2879, 2951
BsmAI GTCTC 8 cut(s) 231, 596, 749, 1028, 1805, 2062, 2702, 3115
BsmBI CGTCTC 2 cut(s) 231, 2062
BsmFI GGGAC 1 cut(s) 904
BsmI GAATGC 1 cut(s) 2277
Bso31I GGTCTC 5 cut(s) 596, 1028, 1805, 2702, 3115
BsoBI CYCGRG 1 cut(s) 1038
Bsp119I TTCGAA 1 cut(s) 2723
Bsp1286I GDGCHC 5 cut(s) 120, 289, 2415, 2446, 3060
Bsp19I CCATGG 2 cut(s) 559, 2553
BspCNI CTCAG 9 cut(s) 35, 282, 314, 471, 1294, 2371, 2846, 2982, 3249
BspFNI CGCG 2 cut(s) 251, 318
BspHI TCATGA 1 cut(s) 1255
BspLI GGNNCC 6 cut(s) 68, 104, 274, 1351, 1357, 2884
BspMI ACCTGC 1 cut(s) 3271
BspPI GGATC 5 cut(s) 878, 1271, 2004, 2103, 2754
BspT104I TTCGAA 1 cut(s) 2723
BspT107I GGYRCC 2 cut(s) 66, 272
BspTNI GGTCTC 5 cut(s) 596, 1028, 1805, 2702, 3115
BsrBI CCGCTC 2 cut(s) 193, 245
BsrDI GCAATG 3 cut(s) 904, 1397, 2623
BsrFI RCCGGY 2 cut(s) 278, 1352
BsrI ACTGG 1 cut(s) 3050
BssAI RCCGGY 2 cut(s) 278, 1352
BssECI CCNNGG 7 cut(s) 345, 559, 1110, 1516, 2553, 2950, 3191
BssNI GRCGYC 3 cut(s) 166, 273, 1106
BssT1I CCWWGG 5 cut(s) 559, 1110, 2553, 2950, 3191
Bst2UI CCWGG 7 cut(s) 888, 954, 1281, 1347, 1517, 1665, 2493
Bst4CI ACNGT 9 cut(s) 640, 682, 1125, 1246, 1269, 1589, 1960, 2176, 3035
Bst6I CTCTTC 5 cut(s) 302, 384, 972, 1915, 2867
BstACI GRCGYC 3 cut(s) 166, 273, 1106
BstAPI GCANNNNNTGC 1 cut(s) 1087
BstBI TTCGAA 1 cut(s) 2723
BstC8I GCNNGC 5 cut(s) 120, 280, 1560, 1728, 2452
BstDSI CCRYGG 2 cut(s) 559, 2553
BstEII GGTNACC 1 cut(s) 2528
BstF5I GGATG 4 cut(s) 796, 1189, 1765, 2860
BstFNI CGCG 2 cut(s) 251, 318
BstH2I RGCGCY 2 cut(s) 276, 3221
BstHHI GCGC 3 cut(s) 275, 318, 3220
BstMAI GTCTC 8 cut(s) 231, 596, 749, 1028, 1805, 2062, 2702, 3115
BstMCI CGRYCG 3 cut(s) 97, 2907, 3034
BstNI CCWGG 7 cut(s) 888, 954, 1281, 1347, 1517, 1665, 2493
BstNSI RCATGY 7 cut(s) 721, 919, 1144, 1501, 2050, 2234, 2363
BstPAI GACNNNNGTC 1 cut(s) 1040
BstPI GGTNACC 1 cut(s) 2528
BstSCI CCNGG 8 cut(s) 141, 886, 952, 1279, 1345, 1515, 1663, 2491
BstSFI CTRYAG 1 cut(s) 3061
BstSLI GKGCMC 1 cut(s) 289
BstUI CGCG 2 cut(s) 251, 318
BstV1I GCAGC 5 cut(s) 936, 1102, 1312, 1354, 2625
BstV2I GAAGAC 1 cut(s) 2009
BstX2I RGATCY 4 cut(s) 883, 1276, 2746, 2867
BstYI RGATCY 4 cut(s) 883, 1276, 2746, 2867
BstZI CGGCCG 2 cut(s) 94, 2904
Bsu36I CCTNAGG 2 cut(s) 1458, 2379
BtgI CCRYGG 2 cut(s) 559, 2553
BtrI CACGTC 1 cut(s) 490
BtsCI GGATG 4 cut(s) 796, 1189, 1765, 2860
BtsI GCAGTG 1 cut(s) 3004
BtsIMutI CAGTG 5 cut(s) 287, 687, 2172, 3004, 3057
BveI ACCTGC 1 cut(s) 3271
Cac8I GCNNGC 5 cut(s) 120, 280, 1560, 1728, 2452
CciI TCATGA 1 cut(s) 1255
CfoI GCGC 3 cut(s) 275, 318, 3220
Cfr10I RCCGGY 2 cut(s) 278, 1352
Cfr13I GGNCC 4 cut(s) 102, 160, 551, 2882
CseI GACGC 2 cut(s) 307, 1095
Csp6I GTAC 8 cut(s) 522, 698, 1121, 1478, 1596, 2018, 2474, 3066
CspCI CAANNNNNGTGG 2 cut(s) 471, 506
CviQI GTAC 8 cut(s) 522, 698, 1121, 1478, 1596, 2018, 2474, 3066
DinI GGCGCC 1 cut(s) 274
DraI TTTAAA 1 cut(s) 2388
EaeI YGGCCR 4 cut(s) 94, 348, 738, 2904
EagI CGGCCG 2 cut(s) 94, 2904
Eam1104I CTCTTC 5 cut(s) 302, 384, 972, 1915, 2867
EarI CTCTTC 5 cut(s) 302, 384, 972, 1915, 2867
Ecl136II GAGCTC 3 cut(s) 118, 2413, 3058
EclXI CGGCCG 2 cut(s) 94, 2904
Eco130I CCWWGG 5 cut(s) 559, 1110, 2553, 2950, 3191
Eco147I AGGCCT 1 cut(s) 2377
Eco24I GRGCYC 3 cut(s) 120, 2415, 3060
Eco31I GGTCTC 5 cut(s) 596, 1028, 1805, 2702, 3115
Eco32I GATATC 1 cut(s) 2896
Eco47I GGWCC 2 cut(s) 102, 160
Eco47III AGCGCT 1 cut(s) 3219
Eco52I CGGCCG 2 cut(s) 94, 2904
Eco53kI GAGCTC 3 cut(s) 118, 2413, 3058
Eco57I CTGAAG 3 cut(s) 1726, 2848, 3257
Eco81I CCTNAGG 2 cut(s) 1458, 2379
Eco88I CYCGRG 1 cut(s) 1038
Eco91I GGTNACC 1 cut(s) 2528
EcoICRI GAGCTC 3 cut(s) 118, 2413, 3058
EcoO109I RGGNCCY 1 cut(s) 2882
EcoO65I GGTNACC 1 cut(s) 2528
EcoRI GAATTC 1 cut(s) 1838
EcoRII CCWGG 7 cut(s) 886, 952, 1279, 1345, 1515, 1663, 2491
EcoRV GATATC 1 cut(s) 2896
EcoT14I CCWWGG 5 cut(s) 559, 1110, 2553, 2950, 3191
EcoT22I ATGCAT 3 cut(s) 1146, 1503, 2052
EcoT38I GRGCYC 3 cut(s) 120, 2415, 3060
EgeI GGCGCC 1 cut(s) 274
EheI GGCGCC 1 cut(s) 274
ErhI CCWWGG 5 cut(s) 559, 1110, 2553, 2950, 3191
Esp3I CGTCTC 2 cut(s) 231, 2062
FalI AAGNNNNNCTT 2 cut(s) 1070, 1102
FaqI GGGAC 1 cut(s) 904
FauI CCCGC 1 cut(s) 114
FauNDI CATATG 1 cut(s) 444
FbaI TGATCA 1 cut(s) 2304
Fnu4HI GCNGC 9 cut(s) 97, 123, 191, 276, 950, 1091, 1326, 1343, 2614
FokI GGATG 4 cut(s) 803, 1196, 1772, 2867
FriOI GRGCYC 3 cut(s) 120, 2415, 3060
Fsp4HI GCNGC 9 cut(s) 97, 123, 191, 276, 950, 1091, 1326, 1343, 2614
FspBI CTAG 3 cut(s) 752, 1943, 3192
GlaI GCGC 3 cut(s) 274, 317, 3219
GluI GCNGC 9 cut(s) 97, 123, 191, 276, 950, 1091, 1326, 1343, 2614
GsaI CCCAGC 1 cut(s) 552
GsuI CTGGAG 2 cut(s) 949, 1342
HaeII RGCGCY 2 cut(s) 276, 3221
HapII CCGG 8 cut(s) 100, 143, 270, 279, 1353, 2416, 2907, 3125
HgaI GACGC 2 cut(s) 307, 1095
HhaI GCGC 3 cut(s) 275, 318, 3220
Hin1I GRCGYC 3 cut(s) 166, 273, 1106
Hin6I GCGC 3 cut(s) 273, 316, 3218
HinP1I GCGC 3 cut(s) 273, 316, 3218
HincII GTYRAC 1 cut(s) 2257
HindII GTYRAC 1 cut(s) 2257
HindIII AAGCTT 1 cut(s) 2627
HpaI GTTAAC 1 cut(s) 2257
HpaII CCGG 8 cut(s) 100, 143, 270, 279, 1353, 2416, 2907, 3125
HphI GGTGA 7 cut(s) 22, 771, 914, 1164, 2054, 2966, 3196
Hpy166II GTNNAC 9 cut(s) 287, 764, 1978, 2020, 2179, 2228, 2257, 2957, 3187
Hpy188III TCNNGA 8 cut(s) 966, 1229, 1256, 1359, 1914, 1943, 2326, 2592
Hpy8I GTNNAC 9 cut(s) 287, 764, 1978, 2020, 2179, 2228, 2257, 2957, 3187
Hpy99I CGWCG 4 cut(s) 168, 182, 209, 323
HpyCH4III ACNGT 9 cut(s) 640, 682, 1125, 1246, 1269, 1589, 1960, 2176, 3035
HpyCH4IV ACGT 4 cut(s) 166, 216, 489, 2843
HpySE526I ACGT 4 cut(s) 166, 216, 489, 2843
Hsp92I GRCGYC 3 cut(s) 166, 273, 1106
HspAI GCGC 3 cut(s) 273, 316, 3218
KasI GGCGCC 1 cut(s) 272
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Ksp22I TGATCA 1 cut(s) 2304
KspAI GTTAAC 1 cut(s) 2257
LmnI GCTCC 7 cut(s) 179, 398, 1361, 1490, 2418, 3055, 3244
Lsp1109I GCAGC 5 cut(s) 936, 1102, 1312, 1354, 2625
LweI GCATC 3 cut(s) 119, 1627, 2572
MaeI CTAG 3 cut(s) 752, 1943, 3192
MaeII ACGT 4 cut(s) 166, 216, 489, 2843
MaeIII GTNAC 8 cut(s) 217, 238, 485, 777, 1583, 2042, 2528, 2911
MbiI CCGCTC 2 cut(s) 193, 245
MflI RGATCY 4 cut(s) 883, 1276, 2746, 2867
MhlI GDGCHC 5 cut(s) 120, 289, 2415, 2446, 3060
MlsI TGGCCA 1 cut(s) 740
MluNI TGGCCA 1 cut(s) 740
Mly113I GGCGCC 1 cut(s) 273
MlyI GAGTC 5 cut(s) 14, 44, 263, 1050, 2919
MmeI TCCRAC 5 cut(s) 72, 78, 187, 228, 701
Mox20I TGGCCA 1 cut(s) 740
Mph1103I ATGCAT 3 cut(s) 1146, 1503, 2052
MreI CGCCGGCG 1 cut(s) 278
MroNI GCCGGC 1 cut(s) 278
MscI TGGCCA 1 cut(s) 740
MseI TTAA 8 cut(s) 1367, 2055, 2220, 2256, 2387, 2487, 3087, 3222
MslI CAYNNNNRTG 4 cut(s) 2055, 2235, 2481, 3033
Msp20I TGGCCA 1 cut(s) 740
MspI CCGG 8 cut(s) 100, 143, 270, 279, 1353, 2416, 2907, 3125
MspR9I CCNGG 8 cut(s) 143, 888, 954, 1281, 1347, 1517, 1665, 2493
Mva1269I GAATGC 1 cut(s) 2277
MvaI CCWGG 7 cut(s) 888, 954, 1281, 1347, 1517, 1665, 2493
MvnI CGCG 2 cut(s) 251, 318
NaeI GCCGGC 1 cut(s) 280
NarI GGCGCC 1 cut(s) 273
NciI CCSGG 1 cut(s) 143
NcoI CCATGG 2 cut(s) 559, 2553
NdeI CATATG 1 cut(s) 444
NgoMIV GCCGGC 1 cut(s) 278
NlaIV GGNNCC 6 cut(s) 68, 104, 274, 1351, 1357, 2884
NmeAIII GCCGAG 2 cut(s) 72, 326
NmuCI GTSAC 5 cut(s) 217, 485, 777, 2042, 2911
NsiI ATGCAT 3 cut(s) 1146, 1503, 2052
NspI RCATGY 7 cut(s) 721, 919, 1144, 1501, 2050, 2234, 2363
NspV TTCGAA 1 cut(s) 2723
OliI CACNNNNGTG 1 cut(s) 3033
PagI TCATGA 1 cut(s) 1255
PceI AGGCCT 1 cut(s) 2377
PciI ACATGT 1 cut(s) 915
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 255
PctI GAATGC 1 cut(s) 2277
PdiI GCCGGC 1 cut(s) 280
PfeI GAWTC 7 cut(s) 860, 957, 1306, 1388, 2129, 2789, 3038
PflMI CCANNNNNTGG 2 cut(s) 560, 1993
PkrI GCNGC 9 cut(s) 98, 124, 192, 277, 951, 1092, 1327, 1344, 2615
PleI GAGTC 5 cut(s) 14, 44, 262, 1049, 2918
PluTI GGCGCC 1 cut(s) 276
PpsI GAGTC 5 cut(s) 14, 44, 262, 1049, 2918
PscI ACATGT 1 cut(s) 915
PshAI GACNNNNGTC 1 cut(s) 1040
PshBI ATTAAT 1 cut(s) 2055
PsiI TTATAA 1 cut(s) 1022
Psp124BI GAGCTC 3 cut(s) 120, 2415, 3060
Psp6I CCWGG 7 cut(s) 886, 952, 1279, 1345, 1515, 1663, 2491
PspEI GGTNACC 1 cut(s) 2528
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 7 cut(s) 886, 952, 1279, 1345, 1515, 1663, 2491
PspN4I GGNNCC 6 cut(s) 68, 104, 274, 1351, 1357, 2884
PspPI GGNCC 4 cut(s) 102, 160, 551, 2882
PsuI RGATCY 4 cut(s) 883, 1276, 2746, 2867
RsaI GTAC 8 cut(s) 523, 699, 1122, 1479, 1597, 2019, 2475, 3067
RsaNI GTAC 8 cut(s) 522, 698, 1121, 1478, 1596, 2018, 2474, 3066
RseI CAYNNNNRTG 4 cut(s) 2055, 2235, 2481, 3033
SacI GAGCTC 3 cut(s) 120, 2415, 3060
SaqAI TTAA 8 cut(s) 1367, 2055, 2220, 2256, 2387, 2487, 3087, 3222
SatI GCNGC 9 cut(s) 97, 123, 191, 276, 950, 1091, 1326, 1343, 2614
Sau96I GGNCC 4 cut(s) 102, 160, 551, 2882
ScaI AGTACT 4 cut(s) 523, 699, 1122, 1479
SchI GAGTC 5 cut(s) 14, 44, 263, 1050, 2919
ScrFI CCNGG 8 cut(s) 143, 888, 954, 1281, 1347, 1517, 1665, 2493
SduI GDGCHC 5 cut(s) 120, 289, 2415, 2446, 3060
SfaNI GCATC 3 cut(s) 119, 1627, 2572
SfcI CTRYAG 1 cut(s) 3061
SfoI GGCGCC 1 cut(s) 274
SfuI TTCGAA 1 cut(s) 2723
SgrAI CRCCGGYG 1 cut(s) 278
SinI GGWCC 2 cut(s) 102, 160
SmiMI CAYNNNNRTG 4 cut(s) 2055, 2235, 2481, 3033
SmlI CTYRAG 1 cut(s) 1031
SmoI CTYRAG 1 cut(s) 1031
SseBI AGGCCT 1 cut(s) 2377
SspDI GGCGCC 1 cut(s) 272
SspMI CTAG 3 cut(s) 752, 1943, 3192
SstI GAGCTC 3 cut(s) 120, 2415, 3060
StuI AGGCCT 1 cut(s) 2377
StyD4I CCNGG 8 cut(s) 141, 886, 952, 1279, 1345, 1515, 1663, 2491
StyI CCWWGG 5 cut(s) 559, 1110, 2553, 2950, 3191
TaaI ACNGT 9 cut(s) 640, 682, 1125, 1246, 1269, 1589, 1960, 2176, 3035
TaiI ACGT 4 cut(s) 169, 219, 492, 2846
TaqI TCGA 4 cut(s) 18, 1391, 2723, 3159
TaqII GACCGA 1 cut(s) 620
TatI WGTACW 6 cut(s) 521, 697, 1120, 1477, 2473, 3065
TauI GCSGC 4 cut(s) 99, 125, 193, 278
TfiI GAWTC 7 cut(s) 860, 957, 1306, 1388, 2129, 2789, 3038
Tru1I TTAA 8 cut(s) 1367, 2055, 2220, 2256, 2387, 2487, 3087, 3222
Tru9I TTAA 8 cut(s) 1367, 2055, 2220, 2256, 2387, 2487, 3087, 3222
TscAI CASTG 5 cut(s) 294, 687, 2179, 3004, 3057
TseFI GTSAC 5 cut(s) 217, 485, 777, 2042, 2911
TseI GCWGC 5 cut(s) 949, 1090, 1325, 1342, 2613
Tsp45I GTSAC 5 cut(s) 217, 485, 777, 2042, 2911
TspGWI ACGGA 3 cut(s) 166, 247, 370
TspRI CASTG 5 cut(s) 294, 687, 2179, 3004, 3057
Van91I CCANNNNNTGG 2 cut(s) 560, 1993
VneI GTGCAC 1 cut(s) 285
VpaK11BI GGWCC 2 cut(s) 102, 160
VspI ATTAAT 1 cut(s) 2055
XapI RAATTY 6 cut(s) 823, 1689, 1838, 2122, 2335, 2644
XbaI TCTAGA 1 cut(s) 1942
XceI RCATGY 7 cut(s) 721, 919, 1144, 1501, 2050, 2234, 2363
XcmI CCANNNNNNNNNTGG 1 cut(s) 640
XmaJI CCTAGG 1 cut(s) 3191
XspI CTAG 3 cut(s) 752, 1943, 3192
ZraI GACGTC 1 cut(s) 167
ZrmI AGTACT 4 cut(s) 523, 699, 1122, 1479
Zsp2I ATGCAT 3 cut(s) 1146, 1503, 2052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.