Rh7BG215900

serine threonine-protein kinase cdc7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0002088
Physical Location & Seq
Forward (+)
18452079 .. 18458160
6082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG215900.1

Sequence Viewer

Length: 3600 bp
ATGGCAACTAACCCACTCGACTCAGCCCTCACCGCCTCAACCATCTCCGACTCCGACAAGGCCTGGCACCTCCTCGCCCTCCTCTTATCTCTCGGCCGCCGCACCCCGCCCTCCGAGCTCGCCGCCCGATGCACGGCGTTCCCGGCAACTACTCATTTGGTCCGACGCCTTTGCTCCGTCGCCAATTCGCCGCTTTCCTTGTCCGACGATCTATACGTCACGCCGTCTCTACTCGCCGTAACCGCTCTCGCGGAGTCCGTTTCATGTTCCGGCGGTGCACTGAGAACCTACTCAGGGAAGAGGAAGCGCGTCGTTTTGGATTGCGGATTTGTGCCCTCGGCCAAACGGAGATTGATTGTGGATTTTGAAAATGTAAATGAAGAGAAAGGAGCGCGGAAATCAGTCGTAATTTCTCCTCCAAAGATACATTTTCATATGACTGACTACCTCAGCAGAAAAATAAATATGCTACCAAGTGATGTGGCTGTGGGGTTGAATAAACCGATGTTCAGTTCTCCATTATTTATTGAATATAGTGCTGAGCCATCCAGTGGTGGAGTGAAGAACATTGATAATGTAGAAGATCAAATGGAGACCGATCTAACTCTGCAAGGGAACATTAACAAAACAGTTATGGCCTGTGTAGATGATTTAGAGCCAACTGTTCCAACAGTGTCAGAAGATTTAGTACTTTATGAAGAAGCTAACATGCACGAGATAGATAGCTTGGCCAAATCTTACCTAGAGAAAAGTAAAGAAAGCATTTTGTCACCTGTGCTGGATGAGAATGTTAGCGGTAGTTGTAAAGTTGCAAATTCTACAATCCTTGAAGATGAAAGAATTGTGGCAGATTCGGAGGAAGAATTGTTAGTAGATCCAGGCAACCACAATGGTGAAAGAGAAAACATGTCCCACCCACAAACTGCTGTCTCTGATAAGCTGCCTGGAATCGACTCCAGAAGATTAGAAGAGGAGGACTATGAAGATACAATACCTTTGAGAAAAAGGCTTATAAGAATACTTGAGACCCGAGTCAAACTTCCAGTTACTGATTTCTCTGTTGCTGAAAAGCAATTGTTGCAGCCATTTGAACAGGTGTCCAAGGATTTAGTACTCTATGAAAGAGCTAACATGCATGAGAAAACCAAAGAAAGCATAATCTCACCAGCTTTGGATGAGAGTGTTAGTGGTAGTCGTAAAGTTACAAACGCTACAATTCTTGAAGATGTAAGAACTGTGGGAAATCATGAGGAAGAACTGTTCATAGATCCTGGCAACCCTAATGGAGAGACTGAGAATCTATCACAGACATTTGCTGCTGTTTCTGATAAGCAGCCTGGAACCGGCTCCAGAAGCTTAAAAGAGCAGGATTTTGAAGATACGATACCATTGCATAAAGAGCGACATGAGAACCAAACCATTTGTGTGGCCACTGATCTCTCCTCTCCTAAGGTGTCCAATGATTTAGTACTCTATGAAGGAGCTGACATGCATGAGATAGATATCCAGGGAAACTCTTACCAAGAGAAAATCAAAGAAAGCATAATGCCAGCTTTGGATGAGAGTGTTAGTGGTAACTGTAAAGGTACAAATGCTACAATCCTTGAAGATAAACGAATTGTGGGAGATGCAGAGGAAGGATTGTTAGTAAATCCTGGCAACCCTATTGGAGAAACAGAAATTCTATACCAGCCATATACTAATGTTTCTGATAAGCTGGCTGGAATTGACTTCAGAAGATTGAATGAGGATGATTATGAAGATACAACACCTGTGAATAAAAGTCTTATGACAAGAAATGAGATCCAAACCACCTGTCCTGCTACTGAATTCTATGTTCCTCAAAAGCAGTTGTTGAAGTCCTCTGCAACAATGAAGGCCATGAACAAGGATAAAATATCTTCAAGATTGCAATCTCTATATAGTTGTAGTCTAGAGCAAGATCAGACTGTTGCCACTCCCAAAGTGAACCTCCACCATAAAATGGATCAGAAGACAAATAGCATGGTACACAAATCAAAGCAGAATTGTGGTGACATGCATATTAATGAAAGAAAGAGACGATACAAATCTATTTCCTCTAAGGATCAGCCAGAGAAAAAAGAACTTCCAAATTTTGATTCTTATATGGTAGAAGAAGAAGAAGGTTCAGGTGGTTATGGCACTGTTTACAGGGCGAGGAGTAAGAATGACAGGACAAGGGTTGCCATTAAGTGTCCACATGCTAATGCTCATAAGAGCCATGTTAACAATGAGTTGAGAATGCTGGAAAGGTTTGGGGGGAAGAACTTTGTGATCAAATATGAAGGCTGTATCAAGAATGGAAATTCAAGTTGTTTTGTATTACAGCATGTTGAGCATGATAGGCCTGAGGTTTTAAAGAAAGAGATAGATGTTTGTGAGCTCCGGTGGTATGGATATTGCTTGTTTAGAGCACTTGCAAGCCTTCATAAGCAGGGAATCGTACATAGAGATGTTAAACCCGGCAATTTCCTTTTCTCTCGTAAGGCCAACAAGGGTTACCTTATTGATTTCAATCTATCCATGGACTTGCATCAGAAGTATGGAAATAATAGTAAGTCAAGAGGGGGATTTGATGTAAGCAGCAATGATGAGAAGCTTATGACTGCAAAAAATTTACCTCCAATCACACTTAGGAAGTTGCCAAGTGGTAAATCTTTACTAACAGCCAATGGAGAGACCTCAAAAGGTTTGAAATCCACTTTTGATATGAAGGATCTAAAAAAGATGGCTTTCAGCCAAATGAAGGCCAATAATGATTCCGGTAGTAGGAAGGTAATCAAAAGTCAGGGTGCAGATGGCTCAGGCATAACGTCAGTAAAGGATGTTACAAGTACCAGAACTCCTTCGGCAGAAAGGATGAGAGAACCTTTGCCATGCCAAGGAAGGAAAGAACTGATCAGCCTAGTACAGGAAGCAAGGGAGAGTCCAAATCATGAGGCATCAAGTATTTCAGCTCTTATGAGAAAGAGGGTTCCTGCCCCTCCTGTAAATGTTGATGGCAAGCTTTTCCATGTCACTCCGATGCCTATGCACTCAACTACCAGTGGTATTGGTGGTGGTCCATGCTTGATAAGAAGCAGAGGTCTTGGAAAACATAAAGAAGGCCCTTGTGCTGGAACAAAAGGCTTCAGGGCTCCAGAGGTTCTCTTCAGATCTCCACATCAAGGCCCCAAAATTGATATCTGGTCTGCCGGAGTCACGCTACTCTACTTGTTGATTGGCAGAACACCCTTCCTTGGTGAACCTGAACTGAACATCAAGGATATAGCAAAGCTGAGAGGCAGTGAAGATTTATGGGAAGTTGCCAAGCTACACGACCGTGAATCCTCATTTCCAGTGGAGCTCTATAGTACAGATTGTTTGCCCTCTATTAAACTAAAGGAGTGGTGTAGAATGAACACAAAGAGACCAGACTTCTTTGAGCAAATCCCAAGACCCCTTTTCGATCTTGTGGATAAATGCTTGATGGTGAACCCCAGGGTTAGATTGAGTGCAGAGGAAGCGCTTAAACATGAGTTTTTCGCTCCATGCCATGAAGAACTGAGAAAGCACAGGCTTCAAAGGCAGGTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006950 GO:0006974 GO:0007049 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0008360 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010389 GO:0010556 GO:0010557 GO:0010564 GO:0010571 GO:0010604 GO:0010948 GO:0010971 GO:0015630 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0033262 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045171 GO:0045740 GO:0045786 GO:0045787 GO:0045931 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070316 GO:0070317 GO:0071704 GO:0072686 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0098657 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1903047 GO:2000105 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 map04110 map04111 map04113
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1199

Amino Acids

133.82

Weight (kDa)

6.47

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 710 - 855 6.6e-23 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 713 - 858 1.6e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 1046 - 1182 4e-16 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1013
Acc36I ACCTGC 1 cut(s) 3580
AccB1I GGYRCC 1 cut(s) 66
AccB7I CCANNNNNTGG 2 cut(s) 551, 1984
AccBSI CCGCTC 1 cut(s) 245
AccII CGCG 3 cut(s) 251, 309, 394
AclWI GGATC 6 cut(s) 869, 1262, 1798, 1995, 2094, 2745
AcoI YGGCCR 4 cut(s) 94, 339, 729, 1428
AcsI RAATTY 6 cut(s) 814, 1680, 1829, 2113, 2326, 2635
AcuI CTGAAG 3 cut(s) 1717, 3136, 3157
AcyI GRCGYC 1 cut(s) 166
AfaI GTAC 9 cut(s) 690, 1113, 1470, 1588, 2010, 2466, 2857, 2931, 3376
AfeI AGCGCT 1 cut(s) 3528
AflIII ACRYGT 1 cut(s) 906
AjnI CCWGG 8 cut(s) 62, 877, 943, 1270, 1336, 1506, 1654, 3500
AjuI GAANNNNNNNTTGG 2 cut(s) 2104, 2136
AleI CACNNNNGTG 2 cut(s) 891, 3342
AloI GAACNNNNNNTCC 2 cut(s) 1244, 1276
Alw21I GWGCWC 5 cut(s) 120, 280, 2406, 2437, 3369
Alw26I GTCTC 8 cut(s) 231, 587, 934, 1019, 1283, 2053, 2693, 3424
Alw44I GTGCAC 1 cut(s) 276
AlwI GGATC 6 cut(s) 869, 1262, 1798, 1995, 2094, 2745
AlwNI CAGNNNCTG 1 cut(s) 1049
Ama87I CYCGRG 1 cut(s) 1029
Aor51HI AGCGCT 1 cut(s) 3528
ApaLI GTGCAC 1 cut(s) 276
ApeKI GCWGC 5 cut(s) 940, 1081, 1316, 1333, 2604
ApoI RAATTY 6 cut(s) 814, 1680, 1829, 2113, 2326, 2635
ArsI GACNNNNNNTTYG 2 cut(s) 1801, 1833
AseI ATTAAT 1 cut(s) 2046
Asp700I GAANNNNTTC 1 cut(s) 2866
AspLEI GCGC 3 cut(s) 309, 394, 3529
AspS9I GGNCC 4 cut(s) 160, 3083, 3128, 3191
AsuC2I CCSGG 2 cut(s) 143, 2484
AsuHPI GGTGA 7 cut(s) 22, 762, 905, 1155, 2045, 3275, 3505
AvaI CYCGRG 1 cut(s) 1029
AvaII GGWCC 2 cut(s) 160, 3083
AxyI CCTNAGG 2 cut(s) 1449, 2370
BaeGI GKGCMC 2 cut(s) 280, 336
BalI TGGCCA 2 cut(s) 731, 1430
BanI GGYRCC 1 cut(s) 66
BanII GRGCYC 4 cut(s) 120, 2406, 3160, 3369
BarI GAAGNNNNNNTAC 6 cut(s) 672, 704, 975, 1007, 1368, 1400
BauI CACGAG 1 cut(s) 713
BbsI GAAGAC 1 cut(s) 2000
Bbv12I GWGCWC 5 cut(s) 120, 280, 2406, 2437, 3369
BbvCI CCTCAGC 1 cut(s) 449
BbvI GCAGC 5 cut(s) 927, 1093, 1303, 1345, 2616
BccI CCATC 6 cut(s) 50, 553, 2743, 2813, 3014, 3484
BceAI ACGGC 3 cut(s) 150, 208, 221
BcgI CGANNNNNNTGC 6 cut(s) 153, 187, 1372, 1406, 3034, 3068
BciT130I CCWGG 8 cut(s) 64, 879, 945, 1272, 1338, 1508, 1656, 3502
BclI TGATCA 2 cut(s) 2295, 2919
BcnI CCSGG 2 cut(s) 143, 2484
BcoDI GTCTC 8 cut(s) 231, 587, 934, 1019, 1283, 2053, 2693, 3424
BfaI CTAG 3 cut(s) 743, 1934, 2927
BfmI CTRYAG 1 cut(s) 3370
BfoI RGCGCY 1 cut(s) 3530
BfuAI ACCTGC 1 cut(s) 3580
BglII AGATCT 1 cut(s) 3176
BisI GCNGC 9 cut(s) 97, 100, 123, 191, 941, 1082, 1317, 1334, 2605
BlpI GCTNAGC 1 cut(s) 540
BlsI GCNGC 9 cut(s) 98, 101, 124, 192, 942, 1083, 1318, 1335, 2606
BmcAI AGTACT 3 cut(s) 690, 1113, 1470
Bme18I GGWCC 2 cut(s) 160, 3083
BmeT110I CYCGRG 1 cut(s) 1029
BmgT120I GGNCC 4 cut(s) 160, 3083, 3128, 3191
BmiI GGNNCC 6 cut(s) 68, 1342, 1348, 2997, 3159, 3193
BmsI GCATC 5 cut(s) 119, 1618, 2563, 2972, 3036
BoxI GACNNNNGTC 1 cut(s) 1031
BpiI GAAGAC 1 cut(s) 2000
BpmI CTGGAG 3 cut(s) 940, 1333, 3144
Bpu10I CCTNAGC 2 cut(s) 449, 2824
Bpu1102I GCTNAGC 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 1043
BpuMI CCSGG 2 cut(s) 143, 2484
BsaBI GATNNNNATC 4 cut(s) 1502, 1912, 2068, 2535
BsaHI GRCGYC 1 cut(s) 166
BsaI GGTCTC 4 cut(s) 587, 1019, 2693, 3424
BsaJI CCNNGG 8 cut(s) 336, 1101, 1507, 2544, 2902, 3259, 3500, 3501
BsaWI WCCGGW 2 cut(s) 2406, 2783
BsaXI ACNNNNNCTCC 2 cut(s) 2848, 2878
Bse118I RCCGGY 1 cut(s) 1343
Bse1I ACTGG 4 cut(s) 549, 1043, 3066, 3359
Bse21I CCTNAGG 2 cut(s) 1449, 2370
Bse3DI GCAATG 2 cut(s) 1388, 2614
Bse8I GATNNNNATC 4 cut(s) 1502, 1912, 2068, 2535
BseBI CCWGG 8 cut(s) 64, 879, 945, 1272, 1338, 1508, 1656, 3502
BseDI CCNNGG 8 cut(s) 336, 1101, 1507, 2544, 2902, 3259, 3500, 3501
BseGI GGATG 7 cut(s) 545, 787, 1180, 1564, 1756, 2851, 2886
BseJI GATNNNNATC 4 cut(s) 1502, 1912, 2068, 2535
BseMI GCAATG 2 cut(s) 1388, 2614
BseNI ACTGG 4 cut(s) 549, 1043, 3066, 3359
BseRI GAGGAG 6 cut(s) 62, 71, 405, 986, 1432, 2194
BseSI GKGCMC 2 cut(s) 280, 336
BseX3I CGGCCG 1 cut(s) 94
BseXI GCAGC 5 cut(s) 927, 1093, 1303, 1345, 2616
BsgI GTGCAG 2 cut(s) 2835, 3537
Bsh1236I CGCG 3 cut(s) 251, 309, 394
Bsh1285I CGRYCG 2 cut(s) 97, 3343
BshNI GGYRCC 1 cut(s) 66
BsiEI CGRYCG 2 cut(s) 97, 3343
BsiHKAI GWGCWC 5 cut(s) 120, 280, 2406, 2437, 3369
BsiHKCI CYCGRG 1 cut(s) 1029
BsiSI CCGG 7 cut(s) 143, 270, 1344, 2407, 2484, 2784, 3216
BslFI GGGAC 1 cut(s) 895
BsmAI GTCTC 8 cut(s) 231, 587, 934, 1019, 1283, 2053, 2693, 3424
BsmBI CGTCTC 2 cut(s) 231, 2053
BsmFI GGGAC 1 cut(s) 895
BsmI GAATGC 1 cut(s) 2268
Bso31I GGTCTC 4 cut(s) 587, 1019, 2693, 3424
BsoBI CYCGRG 1 cut(s) 1029
Bsp1286I GDGCHC 7 cut(s) 120, 280, 336, 2406, 2437, 3160, 3369
Bsp1720I GCTNAGC 1 cut(s) 540
Bsp19I CCATGG 1 cut(s) 2544
BspFNI CGCG 3 cut(s) 251, 309, 394
BspHI TCATGA 2 cut(s) 1246, 2956
BspLI GGNNCC 6 cut(s) 68, 1342, 1348, 2997, 3159, 3193
BspMI ACCTGC 1 cut(s) 3580
BspPI GGATC 6 cut(s) 869, 1262, 1798, 1995, 2094, 2745
BspT107I GGYRCC 1 cut(s) 66
BspTNI GGTCTC 4 cut(s) 587, 1019, 2693, 3424
BsrBI CCGCTC 1 cut(s) 245
BsrDI GCAATG 2 cut(s) 1388, 2614
BsrFI RCCGGY 1 cut(s) 1343
BsrI ACTGG 4 cut(s) 549, 1043, 3066, 3359
BssAI RCCGGY 1 cut(s) 1343
BssECI CCNNGG 8 cut(s) 336, 1101, 1507, 2544, 2902, 3259, 3500, 3501
BssNI GRCGYC 1 cut(s) 166
BssSI CACGAG 1 cut(s) 713
BssT1I CCWWGG 4 cut(s) 1101, 2544, 2902, 3259
Bst2BI CACGAG 1 cut(s) 713
Bst2UI CCWGG 8 cut(s) 64, 879, 945, 1272, 1338, 1508, 1656, 3502
Bst4CI ACNGT 9 cut(s) 631, 664, 673, 1237, 1260, 1580, 1951, 2167, 3344
Bst6I CTCTTC 4 cut(s) 293, 375, 963, 3176
BstACI GRCGYC 1 cut(s) 166
BstAPI GCANNNNNTGC 1 cut(s) 1078
BstC8I GCNNGC 5 cut(s) 120, 1551, 1719, 2443, 3026
BstDSI CCRYGG 1 cut(s) 2544
BstEII GGTNACC 1 cut(s) 2519
BstENI CCTNNNNNAGG 1 cut(s) 2930
BstF5I GGATG 7 cut(s) 545, 787, 1180, 1564, 1756, 2851, 2886
BstFNI CGCG 3 cut(s) 251, 309, 394
BstH2I RGCGCY 1 cut(s) 3530
BstHHI GCGC 3 cut(s) 309, 394, 3529
BstMAI GTCTC 8 cut(s) 231, 587, 934, 1019, 1283, 2053, 2693, 3424
BstMCI CGRYCG 2 cut(s) 97, 3343
BstNI CCWGG 8 cut(s) 64, 879, 945, 1272, 1338, 1508, 1656, 3502
BstNSI RCATGY 7 cut(s) 712, 910, 1135, 1492, 2041, 2225, 2354
BstPAI GACNNNNGTC 1 cut(s) 1031
BstPI GGTNACC 1 cut(s) 2519
BstSFI CTRYAG 1 cut(s) 3370
BstSLI GKGCMC 2 cut(s) 280, 336
BstUI CGCG 3 cut(s) 251, 309, 394
BstV1I GCAGC 5 cut(s) 927, 1093, 1303, 1345, 2616
BstV2I GAAGAC 1 cut(s) 2000
BstX2I RGATCY 5 cut(s) 874, 1267, 1803, 2737, 3176
BstXI CCANNNNNNTGG 1 cut(s) 1426
BstYI RGATCY 5 cut(s) 874, 1267, 1803, 2737, 3176
BstZI CGGCCG 1 cut(s) 94
Bsu36I CCTNAGG 2 cut(s) 1449, 2370
BtgI CCRYGG 1 cut(s) 2544
BtsCI GGATG 7 cut(s) 545, 787, 1180, 1564, 1756, 2851, 2886
BtsI GCAGTG 1 cut(s) 3313
BtsIMutI CAGTG 8 cut(s) 278, 556, 678, 1431, 2163, 3073, 3313, 3366
BveI ACCTGC 1 cut(s) 3580
Cac8I GCNNGC 5 cut(s) 120, 1551, 1719, 2443, 3026
CaiI CAGNNNCTG 1 cut(s) 1049
CciI TCATGA 2 cut(s) 1246, 2956
CfoI GCGC 3 cut(s) 309, 394, 3529
Cfr10I RCCGGY 1 cut(s) 1343
Cfr13I GGNCC 4 cut(s) 160, 3083, 3128, 3191
CseI GACGC 2 cut(s) 174, 298
Csp6I GTAC 9 cut(s) 689, 1112, 1469, 1587, 2009, 2465, 2856, 2930, 3375
CspCI CAANNNNNGTGG 2 cut(s) 462, 497
CviQI GTAC 9 cut(s) 689, 1112, 1469, 1587, 2009, 2465, 2856, 2930, 3375
DraI TTTAAA 1 cut(s) 2379
EaeI YGGCCR 4 cut(s) 94, 339, 729, 1428
EagI CGGCCG 1 cut(s) 94
Eam1104I CTCTTC 4 cut(s) 293, 375, 963, 3176
EarI CTCTTC 4 cut(s) 293, 375, 963, 3176
Ecl136II GAGCTC 3 cut(s) 118, 2404, 3367
EclXI CGGCCG 1 cut(s) 94
Eco130I CCWWGG 4 cut(s) 1101, 2544, 2902, 3259
Eco147I AGGCCT 2 cut(s) 62, 2368
Eco24I GRGCYC 4 cut(s) 120, 2406, 3160, 3369
Eco31I GGTCTC 4 cut(s) 587, 1019, 2693, 3424
Eco32I GATATC 2 cut(s) 1504, 3205
Eco47I GGWCC 2 cut(s) 160, 3083
Eco47III AGCGCT 1 cut(s) 3528
Eco52I CGGCCG 1 cut(s) 94
Eco53kI GAGCTC 3 cut(s) 118, 2404, 3367
Eco57I CTGAAG 3 cut(s) 1717, 3136, 3157
Eco81I CCTNAGG 2 cut(s) 1449, 2370
Eco88I CYCGRG 1 cut(s) 1029
Eco91I GGTNACC 1 cut(s) 2519
EcoICRI GAGCTC 3 cut(s) 118, 2404, 3367
EcoNI CCTNNNNNAGG 1 cut(s) 2930
EcoO109I RGGNCCY 2 cut(s) 3128, 3191
EcoO65I GGTNACC 1 cut(s) 2519
EcoRI GAATTC 1 cut(s) 1829
EcoRII CCWGG 8 cut(s) 62, 877, 943, 1270, 1336, 1506, 1654, 3500
EcoRV GATATC 2 cut(s) 1504, 3205
EcoT14I CCWWGG 4 cut(s) 1101, 2544, 2902, 3259
EcoT22I ATGCAT 3 cut(s) 1137, 1494, 2043
EcoT38I GRGCYC 4 cut(s) 120, 2406, 3160, 3369
ErhI CCWWGG 4 cut(s) 1101, 2544, 2902, 3259
Esp3I CGTCTC 2 cut(s) 231, 2053
FaqI GGGAC 1 cut(s) 895
FauI CCCGC 1 cut(s) 114
FauNDI CATATG 1 cut(s) 435
FbaI TGATCA 2 cut(s) 2295, 2919
Fnu4HI GCNGC 9 cut(s) 97, 100, 123, 191, 941, 1082, 1317, 1334, 2605
FokI GGATG 7 cut(s) 532, 794, 1187, 1571, 1763, 2858, 2893
FriOI GRGCYC 4 cut(s) 120, 2406, 3160, 3369
Fsp4HI GCNGC 9 cut(s) 97, 100, 123, 191, 941, 1082, 1317, 1334, 2605
FspBI CTAG 3 cut(s) 743, 1934, 2927
GlaI GCGC 3 cut(s) 308, 393, 3528
GluI GCNGC 9 cut(s) 97, 100, 123, 191, 941, 1082, 1317, 1334, 2605
GsuI CTGGAG 3 cut(s) 940, 1333, 3144
HaeII RGCGCY 1 cut(s) 3530
HapII CCGG 7 cut(s) 143, 270, 1344, 2407, 2484, 2784, 3216
HgaI GACGC 2 cut(s) 174, 298
HhaI GCGC 3 cut(s) 309, 394, 3529
Hin1I GRCGYC 1 cut(s) 166
Hin6I GCGC 3 cut(s) 307, 392, 3527
HinP1I GCGC 3 cut(s) 307, 392, 3527
HincII GTYRAC 1 cut(s) 2248
HindII GTYRAC 1 cut(s) 2248
HindIII AAGCTT 3 cut(s) 1354, 2618, 3026
HpaI GTTAAC 1 cut(s) 2248
HpaII CCGG 7 cut(s) 143, 270, 1344, 2407, 2484, 2784, 3216
HphI GGTGA 7 cut(s) 22, 762, 905, 1155, 2045, 3275, 3505
Hpy166II GTNNAC 8 cut(s) 278, 1969, 2011, 2170, 2219, 2248, 3266, 3496
Hpy8I GTNNAC 8 cut(s) 278, 1969, 2011, 2170, 2219, 2248, 3266, 3496
Hpy99I CGWCG 4 cut(s) 168, 182, 209, 314
HpyCH4III ACNGT 9 cut(s) 631, 664, 673, 1237, 1260, 1580, 1951, 2167, 3344
HpyCH4IV ACGT 2 cut(s) 216, 2834
HpySE526I ACGT 2 cut(s) 216, 2834
Hsp92I GRCGYC 1 cut(s) 166
HspAI GCGC 3 cut(s) 307, 392, 3527
Ksp22I TGATCA 2 cut(s) 2295, 2919
KspAI GTTAAC 1 cut(s) 2248
LmnI GCTCC 8 cut(s) 179, 389, 1352, 1481, 2409, 3163, 3364, 3553
Lsp1109I GCAGC 5 cut(s) 927, 1093, 1303, 1345, 2616
LweI GCATC 5 cut(s) 119, 1618, 2563, 2972, 3036
MaeI CTAG 3 cut(s) 743, 1934, 2927
MaeII ACGT 2 cut(s) 216, 2834
MbiI CCGCTC 1 cut(s) 245
MfeI CAATTG 1 cut(s) 1073
MflI RGATCY 5 cut(s) 874, 1267, 1803, 2737, 3176
MhlI GDGCHC 7 cut(s) 120, 280, 336, 2406, 2437, 3160, 3369
MlsI TGGCCA 2 cut(s) 731, 1430
MluNI TGGCCA 2 cut(s) 731, 1430
MlyI GAGTC 7 cut(s) 14, 44, 263, 947, 1041, 2956, 3228
MmeI TCCRAC 5 cut(s) 72, 78, 187, 228, 692
Mox20I TGGCCA 2 cut(s) 731, 1430
Mph1103I ATGCAT 3 cut(s) 1137, 1494, 2043
MroXI GAANNNNTTC 1 cut(s) 2866
MscI TGGCCA 2 cut(s) 731, 1430
MseI TTAA 9 cut(s) 621, 1358, 2046, 2211, 2247, 2378, 2478, 3396, 3531
MslI CAYNNNNRTG 7 cut(s) 891, 1424, 2046, 2226, 2472, 3044, 3342
Msp20I TGGCCA 2 cut(s) 731, 1430
MspI CCGG 7 cut(s) 143, 270, 1344, 2407, 2484, 2784, 3216
MunI CAATTG 1 cut(s) 1073
Mva1269I GAATGC 1 cut(s) 2268
MvaI CCWGG 8 cut(s) 64, 879, 945, 1272, 1338, 1508, 1656, 3502
MvnI CGCG 3 cut(s) 251, 309, 394
NciI CCSGG 2 cut(s) 143, 2484
NcoI CCATGG 1 cut(s) 2544
NdeI CATATG 1 cut(s) 435
NlaIV GGNNCC 6 cut(s) 68, 1342, 1348, 2997, 3159, 3193
NmeAIII GCCGAG 2 cut(s) 72, 317
NmuCI GTSAC 5 cut(s) 217, 768, 2033, 3037, 3220
NsiI ATGCAT 3 cut(s) 1137, 1494, 2043
NspI RCATGY 7 cut(s) 712, 910, 1135, 1492, 2041, 2225, 2354
OliI CACNNNNGTG 2 cut(s) 891, 3342
PagI TCATGA 2 cut(s) 1246, 2956
PasI CCCWGGG 1 cut(s) 3501
PceI AGGCCT 2 cut(s) 62, 2368
PciI ACATGT 1 cut(s) 906
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 255
PctI GAATGC 1 cut(s) 2268
PdmI GAANNNNTTC 1 cut(s) 2866
PfeI GAWTC 7 cut(s) 851, 948, 1297, 2120, 2460, 2780, 3347
PflMI CCANNNNNTGG 2 cut(s) 551, 1984
PkrI GCNGC 9 cut(s) 98, 101, 124, 192, 942, 1083, 1318, 1335, 2606
PleI GAGTC 7 cut(s) 14, 44, 262, 947, 1040, 2955, 3227
PpsI GAGTC 7 cut(s) 14, 44, 262, 947, 1040, 2955, 3227
PscI ACATGT 1 cut(s) 906
PshAI GACNNNNGTC 1 cut(s) 1031
PshBI ATTAAT 1 cut(s) 2046
PsiI TTATAA 1 cut(s) 1013
Psp124BI GAGCTC 3 cut(s) 120, 2406, 3369
Psp6I CCWGG 8 cut(s) 62, 877, 943, 1270, 1336, 1506, 1654, 3500
PspEI GGTNACC 1 cut(s) 2519
PspGI CCWGG 8 cut(s) 62, 877, 943, 1270, 1336, 1506, 1654, 3500
PspN4I GGNNCC 6 cut(s) 68, 1342, 1348, 2997, 3159, 3193
PspPI GGNCC 4 cut(s) 160, 3083, 3128, 3191
PstNI CAGNNNCTG 1 cut(s) 1049
PsuI RGATCY 5 cut(s) 874, 1267, 1803, 2737, 3176
RsaI GTAC 9 cut(s) 690, 1113, 1470, 1588, 2010, 2466, 2857, 2931, 3376
RsaNI GTAC 9 cut(s) 689, 1112, 1469, 1587, 2009, 2465, 2856, 2930, 3375
RseI CAYNNNNRTG 7 cut(s) 891, 1424, 2046, 2226, 2472, 3044, 3342
SacI GAGCTC 3 cut(s) 120, 2406, 3369
SaqAI TTAA 9 cut(s) 621, 1358, 2046, 2211, 2247, 2378, 2478, 3396, 3531
SatI GCNGC 9 cut(s) 97, 100, 123, 191, 941, 1082, 1317, 1334, 2605
Sau96I GGNCC 4 cut(s) 160, 3083, 3128, 3191
ScaI AGTACT 3 cut(s) 690, 1113, 1470
SchI GAGTC 7 cut(s) 14, 44, 263, 947, 1041, 2956, 3228
SduI GDGCHC 7 cut(s) 120, 280, 336, 2406, 2437, 3160, 3369
SfaNI GCATC 5 cut(s) 119, 1618, 2563, 2972, 3036
SfcI CTRYAG 1 cut(s) 3370
SinI GGWCC 2 cut(s) 160, 3083
SmiMI CAYNNNNRTG 7 cut(s) 891, 1424, 2046, 2226, 2472, 3044, 3342
SmlI CTYRAG 1 cut(s) 1022
SmoI CTYRAG 1 cut(s) 1022
SseBI AGGCCT 2 cut(s) 62, 2368
SspMI CTAG 3 cut(s) 743, 1934, 2927
SstI GAGCTC 3 cut(s) 120, 2406, 3369
StuI AGGCCT 2 cut(s) 62, 2368
StyI CCWWGG 4 cut(s) 1101, 2544, 2902, 3259
TaaI ACNGT 9 cut(s) 631, 664, 673, 1237, 1260, 1580, 1951, 2167, 3344
TaiI ACGT 2 cut(s) 219, 2837
TaqI TCGA 3 cut(s) 18, 951, 3468
TaqII GACCGA 1 cut(s) 611
TatI WGTACW 5 cut(s) 688, 1111, 1468, 2929, 3374
TauI GCSGC 4 cut(s) 99, 102, 125, 193
TfiI GAWTC 7 cut(s) 851, 948, 1297, 2120, 2460, 2780, 3347
Tru1I TTAA 9 cut(s) 621, 1358, 2046, 2211, 2247, 2378, 2478, 3396, 3531
Tru9I TTAA 9 cut(s) 621, 1358, 2046, 2211, 2247, 2378, 2478, 3396, 3531
TscAI CASTG 8 cut(s) 285, 556, 678, 1438, 2170, 3073, 3313, 3366
TseFI GTSAC 5 cut(s) 217, 768, 2033, 3037, 3220
TseI GCWGC 5 cut(s) 940, 1081, 1316, 1333, 2604
Tsp45I GTSAC 5 cut(s) 217, 768, 2033, 3037, 3220
TspGWI ACGGA 3 cut(s) 166, 247, 361
TspRI CASTG 8 cut(s) 285, 556, 678, 1438, 2170, 3073, 3313, 3366
Van91I CCANNNNNTGG 2 cut(s) 551, 1984
VneI GTGCAC 1 cut(s) 276
VpaK11BI GGWCC 2 cut(s) 160, 3083
VspI ATTAAT 1 cut(s) 2046
XagI CCTNNNNNAGG 1 cut(s) 2930
XapI RAATTY 6 cut(s) 814, 1680, 1829, 2113, 2326, 2635
XbaI TCTAGA 1 cut(s) 1933
XceI RCATGY 7 cut(s) 712, 910, 1135, 1492, 2041, 2225, 2354
XmnI GAANNNNTTC 1 cut(s) 2866
XspI CTAG 3 cut(s) 743, 1934, 2927
ZrmI AGTACT 3 cut(s) 690, 1113, 1470
Zsp2I ATGCAT 3 cut(s) 1137, 1494, 2043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.