Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002751

Physical Location & Seq

Genomic Coordinates Forward (+)

20884684 .. 20887610

Gene Structure 2927 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1100700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGATTTGCTTCAACTCAGGCCACTGCTTCTCACTCTACAGCTGTTTGGCAAAGCCTCACAAACCACAATATCGTGGAGGAATCATTGTAAACTCGGAACTTAATGATGGCCTGAAAGGATGGTCTGCTTTCGGCAATGCAAAAATACAACACCGATATTCAGAAGGCAACAAATTCATTGTGGCATATGGTAGAAACCAGCCACATGACAGTATTTCTCAGAAGACCTACTTGCATAGAAACAAGCTCTACACCTTCTCTGTCGATAATTGTACAATATATCTGGTTTGTGTTGGTGCAGCTTGGATACAAGTGAGTAGCGGTAGTGCTCCGGTGACAGCAATTTTCAAGACAAATAGCGTATTTGTACATGCTGGTGCGATTGTAGCCGAATCCGGTTGCTGGTCCATGCTCAAGGGTGGCCTTACTGTCAATGCCACGGGTCCTGCCGAGCTCTACTTTGAGAGCAAGAACGCATCTGTTGGAATCTTTGTTGATAGCATCTCCTTACAACCATTCACTGAGAAGCAGTGGTATTCTCATCAACAACAAAGCATTGAGAAGATTCGTAAGACGAATGTGAGAGTTCAAGCAGTCGATGAGCAAGCAAATCCACTAGAAAATGCAACAATAACCATCCAACAAAAGGCTGCAGGCTTCCCTTTCGGTGTCGCAATCAACAAGAACATCTTAACCAACACGGCCTACCAAAACTGGTTCACTTCAAGGCCCTTCACGGTCACGACATTCGAAGATGAAATGAAATGGTACAGCACGGAGCCTTCTTTGGGCCGTGAGGACTACTCTGCCGCTGATGCCTTGCTTCAGTTTGCAAAACAACACAAGATTGCAGTCCGAGGCCACAACGTGTTCTGGGACGATCCTCATTACCAGCCCAGTTGGCTCAACTCGCTCTCTGGCCGACAATTCTCTGATGCTGCGTACAAGAGGCTCACTTCTGTCATGGGAAGATACAAGGGACAAGTCATTGCTTGGGATGTTTGTAACGAAAATTTGCACTTTAACTTCTTTGAGAGTAAGATGGGTGCAAATGCTTCAGCTGAATTTTACAATTGGGCTAGTAAAGCCGATGGAACAACCACATTGTTTTTGAATGACTACAATACCATTGAGGAGAGTGGAGATCAAGATTCGACGCCGGCTAAGTACCTTCAGAAGCTGCGAGACATACAAGGGTTTCCTGGTAACGAAAATGTGAAGATGGCGATTGGCCTCGAGTCACATTTCAAGACTCCTAACATTCCGTACATAAGATCTTCTATTGATACTCTTGCTGCTGCAAAGGTGCCAATTTGGATTACAGAGTTGGACGTCGAAAGTAGTCCCGATCAGGCATCATACTTGGAGCAGATTCTTAGGGAAGTGCATGCTCACCCTCAGATTCAAGGCATTGTGATTTGGTCCGCATGGAAACCTTGGGGCTGCTACAGGATGTGTTTGACGGACAACAATTTCAAGAACTTGCCCACCGGTGATGTTGTTGACAAGCTCTCACATGAGTTTGGTTTAACTTCTGACGAAATTTTTGTCACGACAGATGCTAATGGCTTCTTCGAAGCCTCCCTTTTCCAGGGCGATTACGAAGTCAAAATTACTCATCCTTTCGTAAACTCCCCCTTGGTTTCGGGTTTGAATGTGGCACCATCTGTCGAGTCCCCGCAACAAATGCAGCTTCAAGTTAATGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005576 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010383 GO:0010410 GO:0016052 GO:0016787 GO:0016798 GO:0031176 GO:0043170 GO:0044036 GO:0044237 GO:0044238 GO:0044260 GO:0045491 GO:0045493 GO:0071554 GO:0071704 GO:0097599 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_4_9 Glyco_hydro_10

Protein Analysis

570

Amino Acids

63.71

Weight (kDa)

6.07

Isoelectric Point (pI)

35.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_10	PF00331	216 - 475	1.3e-41	Glycosyl hydrolase family 10

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002751)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33830 AT4G33840 AT4G33860
fragaria_vesca	FvH4_2g03792 FvH4_2g03793 FvH4_2g03793 FvH4_2g03840 FvH4_3g40060
malus_domestica	MD05G1100700.v1.1 MD10G1105900.v1.1 MD10G1106500.v1.1
prunus_persica	Prupe.8G145100_v2.0.a1 Prupe.8G145200_v2.0.a1 Prupe.8G145300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0072231 RchiOBHm_Chr6g0252841 RchiOBHm_Chr6g0252851
rosa_laevigata	RLG00000015059 RLG00000015061
rosa_multiflora	Rmu_co8129022.1_g000001 Rmu_sc0000714.1_g000002 Rmu_sc0000829.1_g000017 Rmu_sc0000829.1_g000029 Rmu_sc0028322.1_g000001
rosa_roxburghii	Rroxscaffold_7G00212390
rosa_rugosa	Rorug05G0417000 Rorug05G0539100
rosa_samantha	Rh5DG505200 Rh6AG055100 Rh6BG049200 Rh6CG047900 Rh6CG048000 Rh6CG048100 Rh6DG043400 Rh6DG043600 Rh6DG043700
rosa_wichuraiana	Rw5G043950 Rw6G004970 Rw6G004990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1335
AccB1I	GGYRCC	2 cut(s)	1306, 1660
AciI	CCGC	4 cut(s)	321, 810, 1425, 1679
AclWI	GGATC	1 cut(s)	875
AcoI	YGGCCR	1 cut(s)	919
AcsI	RAATTY	4 cut(s)	173, 1012, 1064, 1542
AcuI	CTGAAG	3 cut(s)	809, 1041, 1157
AcyI	GRCGYC	2 cut(s)	1157, 1332
AdeI	CACNNNGTG	1 cut(s)	868
AfaI	GTAC	6 cut(s)	274, 369, 770, 944, 1169, 1268
AfiI	CCNNNNNNNGG	4 cut(s)	402, 646, 788, 1591
AflIII	ACRYGT	1 cut(s)	867
AgeI	ACCGGT	1 cut(s)	1490
AjnI	CCWGG	2 cut(s)	1201, 1590
AluBI	AGCT	8 cut(s)	42, 247, 302, 454, 1061, 1180, 1510, 1693
AluI	AGCT	8 cut(s)	42, 247, 302, 454, 1061, 1180, 1510, 1693
Alw21I	GWGCWC	2 cut(s)	331, 456
Alw26I	GTCTC	1 cut(s)	1179
AlwI	GGATC	1 cut(s)	875
AlwNI	CAGNNNCTG	1 cut(s)	1180
Ama87I	CYCGRG	1 cut(s)	1235
AoxI	GGCC	9 cut(s)	19, 109, 421, 702, 728, 790, 859, 919, 1231
ApeKI	GCWGC	8 cut(s)	299, 650, 938, 1180, 1295, 1298, 1443, 1690
ApoI	RAATTY	4 cut(s)	173, 1012, 1064, 1542
ArsI	GACNNNNNNTTYG	4 cut(s)	1529, 1533, 1561, 1565
AsiGI	ACCGGT	1 cut(s)	1490
AspS9I	GGNCC	5 cut(s)	405, 443, 729, 790, 1422
AsuHPI	GGTGA	3 cut(s)	346, 1385, 1505
AsuII	TTCGAA	2 cut(s)	750, 1575
AvaI	CYCGRG	1 cut(s)	1235
AvaII	GGWCC	3 cut(s)	405, 443, 1422
BanI	GGYRCC	2 cut(s)	1306, 1660
BanII	GRGCYC	1 cut(s)	456
BbsI	GAAGAC	1 cut(s)	230
Bbv12I	GWGCWC	2 cut(s)	331, 456
BbvI	GCAGC	8 cut(s)	311, 637, 925, 1167, 1282, 1285, 1430, 1702
BccI	CCATC	7 cut(s)	101, 114, 644, 1036, 1085, 1216, 1672
BceAI	ACGGC	2 cut(s)	717, 777
BciT130I	CCWGG	2 cut(s)	1203, 1592
BciVI	GTATCC	1 cut(s)	300
BcoDI	GTCTC	1 cut(s)	1179
BfaI	CTAG	2 cut(s)	617, 1080
BfmI	CTRYAG	3 cut(s)	37, 651, 1447
BfuI	GTATCC	1 cut(s)	300
BglI	GCCNNNNNGGC	1 cut(s)	901
BglII	AGATCT	1 cut(s)	1274
BisI	GCNGC	9 cut(s)	300, 651, 810, 939, 1181, 1296, 1299, 1444, 1691
BlsI	GCNGC	9 cut(s)	301, 652, 811, 940, 1182, 1297, 1300, 1445, 1692
Bme1390I	CCNGG	2 cut(s)	1203, 1592
Bme18I	GGWCC	3 cut(s)	405, 443, 1422
BmeT110I	CYCGRG	1 cut(s)	1235
BmgT120I	GGNCC	5 cut(s)	405, 443, 729, 790, 1422
BmiI	GGNNCC	4 cut(s)	444, 780, 1308, 1662
BmrFI	CCNGG	2 cut(s)	1203, 1592
BmrI	ACTGGG	1 cut(s)	891
BmsI	GCATC	6 cut(s)	485, 510, 805, 925, 1364, 1549
BmuI	ACTGGG	1 cut(s)	891
BpiI	GAAGAC	1 cut(s)	230
BplI	GAGNNNNNCTC	2 cut(s)	788, 820
Bpu14I	TTCGAA	2 cut(s)	750, 1575
BpuEI	CTTGAG	1 cut(s)	398
BsaHI	GRCGYC	2 cut(s)	1157, 1332
BsaJI	CCNNGG	5 cut(s)	438, 856, 1436, 1591, 1638
BsaWI	WCCGGW	3 cut(s)	331, 395, 1490
Bsc4I	CCNNNNNNNGG	4 cut(s)	402, 646, 788, 1591
Bse118I	RCCGGY	2 cut(s)	1159, 1490
Bse1I	ACTGG	2 cut(s)	719, 897
Bse3DI	GCAATG	2 cut(s)	142, 987
BseBI	CCWGG	2 cut(s)	1203, 1592
BseDI	CCNNGG	5 cut(s)	438, 856, 1436, 1591, 1638
BseGI	GGATG	5 cut(s)	125, 636, 1003, 1458, 1618
BseLI	CCNNNNNNNGG	4 cut(s)	402, 646, 788, 1591
BseMI	GCAATG	2 cut(s)	142, 987
BseMII	CTCAG	4 cut(s)	30, 233, 513, 1412
BseNI	ACTGG	2 cut(s)	719, 897
BseRI	GAGGAG	1 cut(s)	1148
BseXI	GCAGC	8 cut(s)	311, 637, 925, 1167, 1282, 1285, 1430, 1702
BsgI	GTGCAG	1 cut(s)	318
BshFI	GGCC	9 cut(s)	21, 111, 423, 704, 730, 792, 861, 921, 1233
BshNI	GGYRCC	2 cut(s)	1306, 1660
BshTI	ACCGGT	1 cut(s)	1490
BsiHKAI	GWGCWC	2 cut(s)	331, 456
BsiHKCI	CYCGRG	1 cut(s)	1235
BsiSI	CCGG	4 cut(s)	332, 396, 1160, 1491
BslFI	GGGAC	4 cut(s)	890, 993, 1329, 1660
BslI	CCNNNNNNNGG	4 cut(s)	402, 646, 788, 1591
BsmAI	GTCTC	1 cut(s)	1179
BsmFI	GGGAC	4 cut(s)	890, 993, 1329, 1660
BsnI	GGCC	9 cut(s)	21, 111, 423, 704, 730, 792, 861, 921, 1233
BsoBI	CYCGRG	1 cut(s)	1235
Bsp119I	TTCGAA	2 cut(s)	750, 1575
Bsp1286I	GDGCHC	2 cut(s)	331, 456
Bsp1407I	TGTACA	2 cut(s)	272, 367
Bsp143I	GATC	4 cut(s)	880, 1144, 1274, 1348
BspACI	CCGC	4 cut(s)	321, 810, 1425, 1679
BspANI	GGCC	9 cut(s)	21, 111, 423, 704, 730, 792, 861, 921, 1233
BspCNI	CTCAG	4 cut(s)	29, 232, 514, 1411
BspLI	GGNNCC	4 cut(s)	444, 780, 1308, 1662
BspMAI	CTGCAG	1 cut(s)	655
BspPI	GGATC	1 cut(s)	875
BspT104I	TTCGAA	2 cut(s)	750, 1575
BspT107I	GGYRCC	2 cut(s)	1306, 1660
BsrDI	GCAATG	2 cut(s)	142, 987
BsrFI	RCCGGY	2 cut(s)	1159, 1490
BsrGI	TGTACA	2 cut(s)	272, 367
BsrI	ACTGG	2 cut(s)	719, 897
BssAI	RCCGGY	2 cut(s)	1159, 1490
BssECI	CCNNGG	5 cut(s)	438, 856, 1436, 1591, 1638
BssMI	GATC	4 cut(s)	880, 1144, 1274, 1348
BssNI	GRCGYC	2 cut(s)	1157, 1332
BssT1I	CCWWGG	2 cut(s)	1436, 1638
Bst2UI	CCWGG	2 cut(s)	1203, 1592
Bst4CI	ACNGT	3 cut(s)	212, 430, 739
BstACI	GRCGYC	2 cut(s)	1157, 1332
BstAPI	GCANNNNNTGC	1 cut(s)	1687
BstAUI	TGTACA	2 cut(s)	272, 367
BstBI	TTCGAA	2 cut(s)	750, 1575
BstC8I	GCNNGC	4 cut(s)	606, 655, 1161, 1389
BstDEI	CTNAG	6 cut(s)	16, 219, 522, 1164, 1376, 1398
BstDSI	CCRYGG	1 cut(s)	438
BstENI	CCTNNNNNAGG	1 cut(s)	1589
BstF5I	GGATG	5 cut(s)	125, 636, 1003, 1458, 1618
BstKTI	GATC	4 cut(s)	883, 1147, 1277, 1351
BstMAI	GTCTC	1 cut(s)	1179
BstMBI	GATC	4 cut(s)	880, 1144, 1274, 1348
BstMWI	GCNNNNNNNGC	6 cut(s)	386, 815, 901, 910, 1085, 1687
BstNI	CCWGG	2 cut(s)	1203, 1592
BstNSI	RCATGY	2 cut(s)	374, 1391
BstSCI	CCNGG	2 cut(s)	1201, 1590
BstSFI	CTRYAG	3 cut(s)	37, 651, 1447
BstV1I	GCAGC	8 cut(s)	311, 637, 925, 1167, 1282, 1285, 1430, 1702
BstV2I	GAAGAC	1 cut(s)	230
BstX2I	RGATCY	1 cut(s)	1274
BstYI	RGATCY	1 cut(s)	1274
BsuI	GTATCC	1 cut(s)	300
BsuRI	GGCC	9 cut(s)	21, 111, 423, 704, 730, 792, 861, 921, 1233
BtgI	CCRYGG	1 cut(s)	438
BtsCI	GGATG	5 cut(s)	125, 636, 1003, 1458, 1618
BtsI	GCAGTG	2 cut(s)	22, 536
BtsIMutI	CAGTG	3 cut(s)	22, 519, 536
Cac8I	GCNNGC	4 cut(s)	606, 655, 1161, 1389
CaiI	CAGNNNCTG	1 cut(s)	1180
Cfr10I	RCCGGY	2 cut(s)	1159, 1490
Cfr13I	GGNCC	5 cut(s)	405, 443, 729, 790, 1422
CseI	GACGC	1 cut(s)	1165
Csp6I	GTAC	6 cut(s)	273, 368, 769, 943, 1168, 1267
CspAI	ACCGGT	1 cut(s)	1490
CviAII	CATG	7 cut(s)	206, 371, 409, 964, 1388, 1428, 1517
CviQI	GTAC	6 cut(s)	273, 368, 769, 943, 1168, 1267
DdeI	CTNAG	6 cut(s)	16, 219, 522, 1164, 1376, 1398
DpnI	GATC	4 cut(s)	882, 1146, 1276, 1350
DpnII	GATC	4 cut(s)	880, 1144, 1274, 1348
DraIII	CACNNNGTG	1 cut(s)	868
EaeI	YGGCCR	1 cut(s)	919
Ecl136II	GAGCTC	1 cut(s)	454
Eco130I	CCWWGG	2 cut(s)	1436, 1638
Eco24I	GRGCYC	1 cut(s)	456
Eco47I	GGWCC	3 cut(s)	405, 443, 1422
Eco53kI	GAGCTC	1 cut(s)	454
Eco57I	CTGAAG	3 cut(s)	809, 1041, 1157
Eco88I	CYCGRG	1 cut(s)	1235
EcoICRI	GAGCTC	1 cut(s)	454
EcoNI	CCTNNNNNAGG	1 cut(s)	1589
EcoO109I	RGGNCCY	2 cut(s)	443, 729
EcoRII	CCWGG	2 cut(s)	1201, 1590
EcoT14I	CCWWGG	2 cut(s)	1436, 1638
EcoT38I	GRGCYC	1 cut(s)	456
ErhI	CCWWGG	2 cut(s)	1436, 1638
FaeI	CATG	7 cut(s)	209, 374, 412, 967, 1391, 1431, 1520
FalI	AAGNNNNNCTT	6 cut(s)	215, 247, 674, 706, 1569, 1601
FaqI	GGGAC	4 cut(s)	890, 993, 1329, 1660
FatI	CATG	7 cut(s)	205, 370, 408, 963, 1387, 1427, 1516
FauI	CCCGC	1 cut(s)	1686
FauNDI	CATATG	1 cut(s)	187
Fnu4HI	GCNGC	9 cut(s)	300, 651, 810, 939, 1181, 1296, 1299, 1444, 1691
FokI	GGATG	5 cut(s)	132, 623, 1010, 1465, 1605
FriOI	GRGCYC	1 cut(s)	456
Fsp4HI	GCNGC	9 cut(s)	300, 651, 810, 939, 1181, 1296, 1299, 1444, 1691
FspBI	CTAG	2 cut(s)	617, 1080
GluI	GCNGC	9 cut(s)	300, 651, 810, 939, 1181, 1296, 1299, 1444, 1691
HaeIII	GGCC	9 cut(s)	21, 111, 423, 704, 730, 792, 861, 921, 1233
HapII	CCGG	4 cut(s)	332, 396, 1160, 1491
HgaI	GACGC	1 cut(s)	1165
Hin1I	GRCGYC	2 cut(s)	1157, 1332
Hin1II	CATG	7 cut(s)	209, 374, 412, 967, 1391, 1431, 1520
HincII	GTYRAC	1 cut(s)	1504
HindII	GTYRAC	1 cut(s)	1504
HpaII	CCGG	4 cut(s)	332, 396, 1160, 1491
HphI	GGTGA	3 cut(s)	346, 1385, 1505
Hpy166II	GTNNAC	4 cut(s)	91, 720, 1504, 1630
Hpy188I	TCNGA	8 cut(s)	97, 163, 222, 857, 934, 1176, 1401, 1537
Hpy188III	TCNNGA	7 cut(s)	349, 742, 1148, 1249, 1346, 1477, 1552
Hpy8I	GTNNAC	4 cut(s)	91, 720, 1504, 1630
Hpy99I	CGWCG	2 cut(s)	1159, 1337
HpyAV	CCTTC	5 cut(s)	158, 265, 742, 792, 1181
HpyCH4III	ACNGT	3 cut(s)	212, 430, 739
HpyCH4IV	ACGT	2 cut(s)	867, 1332
HpyF10VI	GCNNNNNNNGC	6 cut(s)	386, 815, 901, 910, 1085, 1687
HpyF3I	CTNAG	6 cut(s)	16, 219, 522, 1164, 1376, 1398
HpySE526I	ACGT	2 cut(s)	867, 1332
Hsp92I	GRCGYC	2 cut(s)	1157, 1332
Hsp92II	CATG	7 cut(s)	209, 374, 412, 967, 1391, 1431, 1520
KroI	GCCGGC	1 cut(s)	1159
KroNI	GCCGGC	1 cut(s)	1161
Kzo9I	GATC	4 cut(s)	880, 1144, 1274, 1348
LmnI	GCTCC	3 cut(s)	334, 778, 1366
Lsp1109I	GCAGC	8 cut(s)	311, 637, 925, 1167, 1282, 1285, 1430, 1702
LweI	GCATC	6 cut(s)	485, 510, 805, 925, 1364, 1549
MaeI	CTAG	2 cut(s)	617, 1080
MaeII	ACGT	2 cut(s)	867, 1332
MaeIII	GTNAC	6 cut(s)	334, 739, 1004, 1205, 1239, 1549
MalI	GATC	4 cut(s)	882, 1146, 1276, 1350
MboI	GATC	4 cut(s)	880, 1144, 1274, 1348
MboII	GAAGA	7 cut(s)	235, 574, 764, 981, 1231, 1269, 1564
MfeI	CAATTG	1 cut(s)	1072
MflI	RGATCY	1 cut(s)	1274
MhlI	GDGCHC	2 cut(s)	331, 456
MlyI	GAGTC	3 cut(s)	1246, 1247, 1682
MmeI	TCCRAC	3 cut(s)	463, 664, 1308
MroNI	GCCGGC	1 cut(s)	1159
MseI	TTAA	5 cut(s)	102, 692, 1023, 1529, 1701
MslI	CAYNNNNRTG	1 cut(s)	375
MspA1I	CMGCKG	3 cut(s)	42, 812, 1061
MspI	CCGG	4 cut(s)	332, 396, 1160, 1491
MspR9I	CCNGG	2 cut(s)	1203, 1592
MunI	CAATTG	1 cut(s)	1072
MvaI	CCWGG	2 cut(s)	1203, 1592
MwoI	GCNNNNNNNGC	6 cut(s)	386, 815, 901, 910, 1085, 1687
NaeI	GCCGGC	1 cut(s)	1161
NdeI	CATATG	1 cut(s)	187
NdeII	GATC	4 cut(s)	880, 1144, 1274, 1348
NgoMIV	GCCGGC	1 cut(s)	1159
NlaIII	CATG	7 cut(s)	209, 374, 412, 967, 1391, 1431, 1520
NlaIV	GGNNCC	4 cut(s)	444, 780, 1308, 1662
NmeAIII	GCCGAG	1 cut(s)	475
NmuCI	GTSAC	4 cut(s)	334, 739, 1239, 1549
NspI	RCATGY	2 cut(s)	374, 1391
NspV	TTCGAA	2 cut(s)	750, 1575
PaeI	GCATGC	1 cut(s)	1391
PaeR7I	CTCGAG	1 cut(s)	1235
PdiI	GCCGGC	1 cut(s)	1161
PfeI	GAWTC	7 cut(s)	81, 392, 486, 565, 1151, 1372, 1402
PinAI	ACCGGT	1 cut(s)	1490
PkrI	GCNGC	9 cut(s)	301, 652, 811, 940, 1182, 1297, 1300, 1445, 1692
PleI	GAGTC	3 cut(s)	1246, 1246, 1681
PpsI	GAGTC	3 cut(s)	1246, 1246, 1681
PpuMI	RGGWCCY	1 cut(s)	443
Psp124BI	GAGCTC	1 cut(s)	456
Psp5II	RGGWCCY	1 cut(s)	443
Psp6I	CCWGG	2 cut(s)	1201, 1590
PspGI	CCWGG	2 cut(s)	1201, 1590
PspN4I	GGNNCC	4 cut(s)	444, 780, 1308, 1662
PspPI	GGNCC	5 cut(s)	405, 443, 729, 790, 1422
PspPPI	RGGWCCY	1 cut(s)	443
PspXI	VCTCGAGB	1 cut(s)	1235
PstI	CTGCAG	1 cut(s)	655
PstNI	CAGNNNCTG	1 cut(s)	1180
PsuI	RGATCY	1 cut(s)	1274
PvuII	CAGCTG	2 cut(s)	42, 1061
RsaI	GTAC	6 cut(s)	274, 369, 770, 944, 1169, 1268
RsaNI	GTAC	6 cut(s)	273, 368, 769, 943, 1168, 1267
RseI	CAYNNNNRTG	1 cut(s)	375
SacI	GAGCTC	1 cut(s)	456
SaqAI	TTAA	5 cut(s)	102, 692, 1023, 1529, 1701
SatI	GCNGC	9 cut(s)	300, 651, 810, 939, 1181, 1296, 1299, 1444, 1691
Sau3AI	GATC	4 cut(s)	880, 1144, 1274, 1348
Sau96I	GGNCC	5 cut(s)	405, 443, 729, 790, 1422
SchI	GAGTC	3 cut(s)	1246, 1247, 1682
ScrFI	CCNGG	2 cut(s)	1203, 1592
SduI	GDGCHC	2 cut(s)	331, 456
SfaNI	GCATC	6 cut(s)	485, 510, 805, 925, 1364, 1549
SfcI	CTRYAG	3 cut(s)	37, 651, 1447
Sfr274I	CTCGAG	1 cut(s)	1235
SfuI	TTCGAA	2 cut(s)	750, 1575
SgrAI	CRCCGGYG	1 cut(s)	1490
SinI	GGWCC	3 cut(s)	405, 443, 1422
SlaI	CTCGAG	1 cut(s)	1235
SmiMI	CAYNNNNRTG	1 cut(s)	375
SmlI	CTYRAG	2 cut(s)	413, 1235
SmoI	CTYRAG	2 cut(s)	413, 1235
SphI	GCATGC	1 cut(s)	1391
SsiI	CCGC	4 cut(s)	321, 810, 1425, 1679
SspMI	CTAG	2 cut(s)	617, 1080
SstI	GAGCTC	1 cut(s)	456
StyD4I	CCNGG	2 cut(s)	1201, 1590
StyI	CCWWGG	2 cut(s)	1436, 1638
TaaI	ACNGT	3 cut(s)	212, 430, 739
TaiI	ACGT	2 cut(s)	870, 1335
TaqI	TCGA	8 cut(s)	264, 597, 750, 1154, 1236, 1335, 1575, 1671
TatI	WGTACW	2 cut(s)	272, 367
TauI	GCSGC	1 cut(s)	812
TfiI	GAWTC	7 cut(s)	81, 392, 486, 565, 1151, 1372, 1402
Tru1I	TTAA	5 cut(s)	102, 692, 1023, 1529, 1701
Tru9I	TTAA	5 cut(s)	102, 692, 1023, 1529, 1701
TscAI	CASTG	3 cut(s)	29, 526, 536
TseFI	GTSAC	4 cut(s)	334, 739, 1239, 1549
TseI	GCWGC	8 cut(s)	299, 650, 938, 1180, 1295, 1298, 1443, 1690
Tsp45I	GTSAC	4 cut(s)	334, 739, 1239, 1549
TspDTI	ATGAA	3 cut(s)	166, 771, 776
TspGWI	ACGGA	3 cut(s)	791, 1254, 1478
TspRI	CASTG	3 cut(s)	29, 526, 536
VpaK11BI	GGWCC	3 cut(s)	405, 443, 1422
XagI	CCTNNNNNAGG	1 cut(s)	1589
XapI	RAATTY	4 cut(s)	173, 1012, 1064, 1542
XceI	RCATGY	2 cut(s)	374, 1391
XhoI	CTCGAG	1 cut(s)	1235
XspI	CTAG	2 cut(s)	617, 1080
ZraI	GACGTC	1 cut(s)	1333

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.