Rh5DG505200

Endo-1,4-beta-xylanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002751
Physical Location & Seq
Forward (+)
79066697 .. 79067840
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG505200.1

Sequence Viewer

Length: 777 bp
ATGCAAAAGGAAACCCTCTACGGAATGCAACAATATCCATCCAACAAAAGGCTCCCCCCTTTCGGATGCGCTATGAACAAGAACATTCTCACCAATACTGCTTACCAAAACTGGTTCACTTCGAGGTTTGGAGTCACAACTTTTGAAGACGAAATGAAATGGTACAGCACAGAACCTTCACAGGGACAAGAGGACTACTCGGTGGCTGATGCCCTGCTTCAGTTTGCTAAACAACAAAACATTGCAGTCAGAGGCCACAATGTGTTCTGGGATGATCCTCAGTACCAACTAGCCATTTTATACAATTGGGCTACTAAAGCTGACGGAGCAACCACCTTGTTCATGAATGACTACAATACCATTGAGGAGAGCAAAGATGGAGCCTCCACCCCGGCTAAGTACCTTCAAAAGCTCAGAGATATACAAGGCTTTCCCGTTAACAACAACTTAAGGATGGCAATAGGACTTGAGTCTCATTTCAAGACTCCTAACATTCCTTACATACGATCTTCTATTGATAGCGCTGCAAACCTAGCTACCAATCTGGATTACAGAATTGGATGTGGAAAGCGGTCCCAATCAAGCAAGGTACTTGGAGCAGATTCTGAGGGAGATACTAATGGTGTCTTCGAGGCCTGCCTTCCCCATGGAGATTATGAAGTGAAAATCACTGACCTGGAATCTGTGAAAGTTTCTACCGGTATGGTTCAGGGATTGAATGTCGCAGCAAGTTCCACTGCACACCAACAACCATTGCTGGTCCAACTTGCTGCATGA

Protein Analysis

258

Amino Acids

28.71

Weight (kDa)

5.4

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 20 - 92 2.7e-16 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 571
AclWI GGATC 1 cut(s) 269
AcuI CTGAAG 1 cut(s) 203
AdeI CACNNNGTG 1 cut(s) 262
AfaI GTAC 4 cut(s) 164, 284, 401, 591
AfeI AGCGCT 1 cut(s) 523
AfiI CCNNNNNNNGG 3 cut(s) 48, 62, 182
AflII CTTAAG 1 cut(s) 448
AgeI ACCGGT 1 cut(s) 698
AgsI TTSAA 4 cut(s) 146, 407, 481, 718
AjnI CCWGG 1 cut(s) 675
AluBI AGCT 3 cut(s) 320, 412, 536
AluI AGCT 3 cut(s) 320, 412, 536
Alw26I GTCTC 1 cut(s) 477
AlwI GGATC 1 cut(s) 269
AlwNI CAGNNNCTG 1 cut(s) 605
Aor51HI AGCGCT 1 cut(s) 523
AoxI GGCC 2 cut(s) 253, 633
ApeKI GCWGC 3 cut(s) 524, 725, 770
AsiGI ACCGGT 1 cut(s) 698
AspLEI GCGC 2 cut(s) 71, 524
AspS9I GGNCC 2 cut(s) 573, 760
AsuC2I CCSGG 1 cut(s) 392
AsuHPI GGTGA 1 cut(s) 82
AvaII GGWCC 2 cut(s) 573, 760
BbsI GAAGAC 2 cut(s) 153, 619
BbvI GCAGC 3 cut(s) 511, 737, 757
BccI CCATC 3 cut(s) 46, 371, 448
BciT130I CCWGG 1 cut(s) 677
BcnI CCSGG 1 cut(s) 392
BcoDI GTCTC 1 cut(s) 477
BfaI CTAG 2 cut(s) 290, 533
BfoI RGCGCY 1 cut(s) 525
BfrI CTTAAG 1 cut(s) 448
BisI GCNGC 3 cut(s) 525, 726, 771
BlsI GCNGC 3 cut(s) 526, 727, 772
Bme1390I CCNGG 2 cut(s) 392, 677
Bme18I GGWCC 2 cut(s) 573, 760
BmgT120I GGNCC 2 cut(s) 573, 760
BmiI GGNNCC 3 cut(s) 53, 382, 575
BmrFI CCNGG 2 cut(s) 392, 677
BmsI GCATC 2 cut(s) 56, 199
BoxI GACNNNNGTC 1 cut(s) 469
BpiI GAAGAC 2 cut(s) 153, 619
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpuEI CTTGAG 1 cut(s) 488
BpuMI CCSGG 1 cut(s) 392
BsaJI CCNNGG 2 cut(s) 390, 646
BsaWI WCCGGW 1 cut(s) 698
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 62, 182
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 1 cut(s) 116
Bse3DI GCAATG 2 cut(s) 240, 752
BseBI CCWGG 1 cut(s) 677
BseDI CCNNGG 2 cut(s) 390, 646
BseGI GGATG 5 cut(s) 38, 71, 277, 459, 566
BseLI CCNNNNNNNGG 3 cut(s) 48, 62, 182
BseMI GCAATG 2 cut(s) 240, 752
BseMII CTCAG 3 cut(s) 293, 427, 597
BseNI ACTGG 1 cut(s) 116
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 3 cut(s) 511, 737, 757
BsgI GTGCAG 1 cut(s) 723
BshFI GGCC 2 cut(s) 255, 635
BshTI ACCGGT 1 cut(s) 698
BsiSI CCGG 2 cut(s) 392, 699
BslFI GGGAC 2 cut(s) 198, 559
BslI CCNNNNNNNGG 3 cut(s) 48, 62, 182
BsmAI GTCTC 1 cut(s) 477
BsmFI GGGAC 2 cut(s) 198, 559
BsmI GAATGC 1 cut(s) 30
BsnI GGCC 2 cut(s) 255, 635
Bsp143I GATC 2 cut(s) 274, 506
Bsp19I CCATGG 1 cut(s) 646
BspACI CCGC 1 cut(s) 571
BspANI GGCC 2 cut(s) 255, 635
BspCNI CTCAG 3 cut(s) 292, 426, 598
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 3 cut(s) 53, 382, 575
BspPI GGATC 1 cut(s) 269
BspTI CTTAAG 1 cut(s) 448
BsrDI GCAATG 2 cut(s) 240, 752
BsrFI RCCGGY 1 cut(s) 698
BsrI ACTGG 1 cut(s) 116
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 2 cut(s) 390, 646
BssMI GATC 2 cut(s) 274, 506
BssT1I CCWWGG 1 cut(s) 646
Bst2UI CCWGG 1 cut(s) 677
BstAFI CTTAAG 1 cut(s) 448
BstC8I GCNNGC 1 cut(s) 637
BstDEI CTNAG 4 cut(s) 279, 396, 413, 606
BstDSI CCRYGG 1 cut(s) 646
BstF5I GGATG 5 cut(s) 38, 71, 277, 459, 566
BstH2I RGCGCY 1 cut(s) 525
BstHHI GCGC 2 cut(s) 71, 524
BstKTI GATC 2 cut(s) 277, 509
BstMAI GTCTC 1 cut(s) 477
BstMBI GATC 2 cut(s) 274, 506
BstMWI GCNNNNNNNGC 3 cut(s) 317, 326, 533
BstNI CCWGG 1 cut(s) 677
BstPAI GACNNNNGTC 1 cut(s) 469
BstSCI CCNGG 2 cut(s) 390, 675
BstV1I GCAGC 3 cut(s) 511, 737, 757
BstV2I GAAGAC 2 cut(s) 153, 619
BsuRI GGCC 2 cut(s) 255, 635
BtgI CCRYGG 1 cut(s) 646
BtsCI GGATG 5 cut(s) 38, 71, 277, 459, 566
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 2 cut(s) 669, 735
Cac8I GCNNGC 1 cut(s) 637
CaiI CAGNNNCTG 1 cut(s) 605
CciI TCATGA 1 cut(s) 342
CfoI GCGC 2 cut(s) 71, 524
Cfr10I RCCGGY 1 cut(s) 698
Cfr13I GGNCC 2 cut(s) 573, 760
Csp6I GTAC 4 cut(s) 163, 283, 400, 590
CspAI ACCGGT 1 cut(s) 698
CviAII CATG 3 cut(s) 343, 647, 774
CviQI GTAC 4 cut(s) 163, 283, 400, 590
DdeI CTNAG 4 cut(s) 279, 396, 413, 606
DpnI GATC 2 cut(s) 276, 508
DpnII GATC 2 cut(s) 274, 506
DraIII CACNNNGTG 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 646
Eco147I AGGCCT 1 cut(s) 635
Eco47I GGWCC 2 cut(s) 573, 760
Eco47III AGCGCT 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 675
EcoT14I CCWWGG 1 cut(s) 646
ErhI CCWWGG 1 cut(s) 646
FaeI CATG 3 cut(s) 346, 650, 777
FaiI YATR 9 cut(s) 74, 301, 344, 422, 503, 648, 657, 704, 775
FaqI GGGAC 2 cut(s) 198, 559
FatI CATG 3 cut(s) 342, 646, 773
Fnu4HI GCNGC 3 cut(s) 525, 726, 771
FokI GGATG 5 cut(s) 25, 78, 284, 466, 573
Fsp4HI GCNGC 3 cut(s) 525, 726, 771
FspBI CTAG 2 cut(s) 290, 533
GlaI GCGC 2 cut(s) 70, 523
GluI GCNGC 3 cut(s) 525, 726, 771
HaeII RGCGCY 1 cut(s) 525
HaeIII GGCC 2 cut(s) 255, 635
HapII CCGG 2 cut(s) 392, 699
HhaI GCGC 2 cut(s) 71, 524
Hin1II CATG 3 cut(s) 346, 650, 777
Hin6I GCGC 2 cut(s) 69, 522
HinP1I GCGC 2 cut(s) 69, 522
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 5 cut(s) 132, 470, 484, 602, 680
HpaI GTTAAC 1 cut(s) 439
HpaII CCGG 2 cut(s) 392, 699
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 2 cut(s) 117, 439
Hpy188I TCNGA 4 cut(s) 65, 251, 416, 607
Hpy188III TCNNGA 3 cut(s) 343, 481, 545
Hpy8I GTNNAC 2 cut(s) 117, 439
HpyAV CCTTC 3 cut(s) 186, 413, 650
HpyCH4V TGCA 6 cut(s) 4, 28, 245, 527, 740, 773
HpyF10VI GCNNNNNNNGC 3 cut(s) 317, 326, 533
HpyF3I CTNAG 4 cut(s) 279, 396, 413, 606
Hsp92II CATG 3 cut(s) 346, 650, 777
HspAI GCGC 2 cut(s) 69, 522
KspAI GTTAAC 1 cut(s) 439
Kzo9I GATC 2 cut(s) 274, 506
LmnI GCTCC 4 cut(s) 57, 326, 380, 596
Lsp1109I GCAGC 3 cut(s) 511, 737, 757
LweI GCATC 2 cut(s) 56, 199
MaeI CTAG 2 cut(s) 290, 533
MaeIII GTNAC 1 cut(s) 133
MalI GATC 2 cut(s) 276, 508
MboI GATC 2 cut(s) 274, 506
MboII GAAGA 3 cut(s) 158, 501, 619
MfeI CAATTG 1 cut(s) 304
MluCI AATT 2 cut(s) 304, 555
MlyI GAGTC 3 cut(s) 141, 478, 479
MmeI TCCRAC 1 cut(s) 66
MnlI CCTC 9 cut(s) 26, 117, 184, 245, 288, 358, 394, 601, 625
MseI TTAA 2 cut(s) 438, 449
MspCI CTTAAG 1 cut(s) 448
MspI CCGG 2 cut(s) 392, 699
MspR9I CCNGG 2 cut(s) 392, 677
MunI CAATTG 1 cut(s) 304
Mva1269I GAATGC 1 cut(s) 30
MvaI CCWGG 1 cut(s) 677
MwoI GCNNNNNNNGC 3 cut(s) 317, 326, 533
NciI CCSGG 1 cut(s) 392
NcoI CCATGG 1 cut(s) 646
NdeII GATC 2 cut(s) 274, 506
NlaIII CATG 3 cut(s) 346, 650, 777
NlaIV GGNNCC 3 cut(s) 53, 382, 575
NmuCI GTSAC 1 cut(s) 133
PagI TCATGA 1 cut(s) 342
PceI AGGCCT 1 cut(s) 635
PctI GAATGC 1 cut(s) 30
PfeI GAWTC 2 cut(s) 602, 680
PinAI ACCGGT 1 cut(s) 698
PkrI GCNGC 3 cut(s) 526, 727, 772
PleI GAGTC 3 cut(s) 140, 478, 478
PpsI GAGTC 3 cut(s) 140, 478, 478
PshAI GACNNNNGTC 1 cut(s) 469
Psp6I CCWGG 1 cut(s) 675
PspGI CCWGG 1 cut(s) 675
PspN4I GGNNCC 3 cut(s) 53, 382, 575
PspPI GGNCC 2 cut(s) 573, 760
PstNI CAGNNNCTG 1 cut(s) 605
RsaI GTAC 4 cut(s) 164, 284, 401, 591
RsaNI GTAC 4 cut(s) 163, 283, 400, 590
SaqAI TTAA 2 cut(s) 438, 449
SatI GCNGC 3 cut(s) 525, 726, 771
Sau3AI GATC 2 cut(s) 274, 506
Sau96I GGNCC 2 cut(s) 573, 760
SchI GAGTC 3 cut(s) 141, 478, 479
ScrFI CCNGG 2 cut(s) 392, 677
SfaNI GCATC 2 cut(s) 56, 199
SinI GGWCC 2 cut(s) 573, 760
SmlI CTYRAG 2 cut(s) 448, 467
SmoI CTYRAG 2 cut(s) 448, 467
Sse9I AATT 2 cut(s) 304, 555
SseBI AGGCCT 1 cut(s) 635
SsiI CCGC 1 cut(s) 571
SspMI CTAG 2 cut(s) 290, 533
StuI AGGCCT 1 cut(s) 635
StyD4I CCNGG 2 cut(s) 390, 675
StyI CCWWGG 1 cut(s) 646
TaqI TCGA 2 cut(s) 122, 630
TasI AATT 2 cut(s) 304, 555
TfiI GAWTC 2 cut(s) 602, 680
Tru1I TTAA 2 cut(s) 438, 449
Tru9I TTAA 2 cut(s) 438, 449
TscAI CASTG 2 cut(s) 676, 742
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 3 cut(s) 524, 725, 770
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 5 cut(s) 89, 170, 331, 359, 672
TspGWI ACGGA 2 cut(s) 36, 339
TspRI CASTG 2 cut(s) 676, 742
Vha464I CTTAAG 1 cut(s) 448
VpaK11BI GGWCC 2 cut(s) 573, 760
XspI CTAG 2 cut(s) 290, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.