MD10G1105900.v1.1

Endo-1,4-beta-xylanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002751
Physical Location & Seq
Forward (+)
17330093 .. 17332002
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1105900.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGCTCAAGGGTGGCTTAACCGTCAATGCCTCAGGTCCCGCCGAGCTCTACTTCGAGAGCGAGAACACATCTGTTGAGATCTTTGTTGATAGCATCTCCTTACAACCATTCACTCAGAAGCAGTGGAATTCTCATCAACAACAAAGCATTGAGAAGGTTCGTAAGACGAATGTTAGAATTCAAGCGGTCACCGAGCAAGGAAATCCACTAGAAAATGCAACAATCATCATCCAACAAAAGGCTCCAGGCTTCCCCTTCGGTGTTGCAGTCAACAAGAACATCCTCACCAACACAGCCTACCAAAACTGGTTCACTTCAAAGCCTTTCAAAGTCACAACGTTTGAAGACGAAATGAAATGGTACACCATGGAGCCTTCTCCAGGCCAAGAGGACTACTCCGCTGCTGATGCCTTGGTTCAGTTTGCAAAGCAACACCAGATTGCAGTCCGAGGCCACAATGTGTTCTGGGAGGATCCTGATTACCAGGCCGGTTGGCTTAACTCGCTCTCTGGCCAACAATTCTCCGATGCCGCTAACAAGAGGCTCAATTCTATTATGGGGAGATACAAGGGACAAGTCATTGCTTGGGATGTTAGCAACGAAAATTTGCATTTTAATTTCTTTGAGAGTAAGATGGGTGCAACTGCGTCTGCTGAATTTTACAATTGGGCTAGTAAAGCTGATGATACAGCCACACTGTTCTTGAATGATTACAATACCATTGAGGAGAGTGGAGATAAAGATTCGATTCCGGCTAAGTACCTTCAGAAGCTGAGAGACATACAAGGGTTTCCCGGTAACAGTAATGCAAAGATGGCGATTGGTCTCGAGTCACATTTCCAGACTCCTAACATTCCATACATAAGATCTTACATTGATACTCTTGCTGGTGCAAATGTGCCAATTTGGATTACAGAATTGGACGTCGTTAGTGGTCCCAATCAGGCATCATACTTAGAGCAGATTCTAAGGGAGGTGCATTCTCACCCTAAGATTCAAGGCATTGTCATTTGGGCTGCATGGAAACCTTCAGGATGCTACAGAATGTGTTTGACGGATAACAATTTCAAGAACTTGCCCACCGGCGATGTTGTCGACAAGCTCACAAGTGAGTTCGGTTTGACTTCGGGCTTAGCTTCTGGCACGACGAACGCAAATGGTTTCTTCGAGGCCTCGCTTTTCCACGGCGATTACGAAGTGAAAATTACTCACCCCTTGGTCGACTCCCCCTTCGTTCGGGGTTTGAATGTGGCACCAACTACTGAGTCCCAGCAACAATTGCACGTCCAACTTAATGCCTGA

Protein Analysis

434

Amino Acids

48.34

Weight (kDa)

5.26

Isoelectric Point (pI)

34.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 82 - 339 2.7e-41 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 927
AccB1I GGYRCC 1 cut(s) 1252
AccI GTMKAC 2 cut(s) 1095, 1221
AciI CCGC 4 cut(s) 39, 185, 399, 531
AclI AACGTT 1 cut(s) 338
AclWI GGATC 2 cut(s) 467, 480
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 4 cut(s) 127, 177, 604, 656
AcuI CTGAAG 2 cut(s) 749, 1014
AcyI GRCGYC 1 cut(s) 924
AdeI CACNNNGTG 1 cut(s) 460
AfaI GTAC 2 cut(s) 362, 761
AfiI CCNNNNNNNGG 3 cut(s) 238, 380, 1236
AgsI TTSAA 8 cut(s) 182, 318, 328, 344, 706, 998, 1069, 1246
AjiI CACGTC 1 cut(s) 1285
AjnI CCWGG 3 cut(s) 244, 379, 483
AluBI AGCT 5 cut(s) 46, 680, 772, 1102, 1136
AluI AGCT 5 cut(s) 46, 680, 772, 1102, 1136
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 2 cut(s) 771, 830
AlwI GGATC 2 cut(s) 467, 480
AlwNI CAGNNNCTG 1 cut(s) 772
Ama87I CYCGRG 1 cut(s) 827
AoxI GGCC 5 cut(s) 382, 451, 486, 511, 1170
ApeKI GCWGC 2 cut(s) 401, 1016
ApoI RAATTY 4 cut(s) 127, 177, 604, 656
ArsI GACNNNNNNTTYG 2 cut(s) 1214, 1246
AspS9I GGNCC 2 cut(s) 35, 935
AsuC2I CCSGG 1 cut(s) 795
AsuHPI GGTGA 4 cut(s) 181, 277, 977, 1202
AvaI CYCGRG 1 cut(s) 827
AvaII GGWCC 2 cut(s) 35, 935
AxyI CCTNAGG 1 cut(s) 31
BalI TGGCCA 1 cut(s) 513
BamHI GGATCC 1 cut(s) 472
BanI GGYRCC 1 cut(s) 1252
BanII GRGCYC 1 cut(s) 48
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 2 cut(s) 388, 1003
BccI CCATC 2 cut(s) 628, 808
BceAI ACGGC 1 cut(s) 1201
BciT130I CCWGG 3 cut(s) 246, 381, 485
BcnI CCSGG 1 cut(s) 795
BcoDI GTCTC 2 cut(s) 771, 830
BfaI CTAG 2 cut(s) 209, 672
BfmI CTRYAG 1 cut(s) 1039
BglII AGATCT 2 cut(s) 78, 866
BisI GCNGC 3 cut(s) 402, 531, 1017
BlpI GCTNAGC 1 cut(s) 1132
BlsI GCNGC 3 cut(s) 403, 532, 1018
Bme1390I CCNGG 4 cut(s) 246, 381, 485, 795
Bme18I GGWCC 2 cut(s) 35, 935
BmeT110I CYCGRG 1 cut(s) 827
BmgBI CACGTC 1 cut(s) 1285
BmgT120I GGNCC 2 cut(s) 35, 935
BmiI GGNNCC 6 cut(s) 37, 243, 372, 474, 937, 1254
BmrFI CCNGG 4 cut(s) 246, 381, 485, 795
BmsI GCATC 5 cut(s) 102, 397, 517, 956, 1025
BpiI GAAGAC 1 cut(s) 351
BplI GAGNNNNNCTC 2 cut(s) 380, 412
BpmI CTGGAG 2 cut(s) 228, 363
Bpu1102I GCTNAGC 1 cut(s) 1132
BpuMI CCSGG 1 cut(s) 795
BsaHI GRCGYC 1 cut(s) 924
BsaI GGTCTC 1 cut(s) 830
BsaJI CCNNGG 5 cut(s) 366, 411, 448, 1183, 1215
Bsc4I CCNNNNNNNGG 3 cut(s) 238, 380, 1236
Bse118I RCCGGY 2 cut(s) 488, 1082
Bse1I ACTGG 1 cut(s) 311
Bse21I CCTNAGG 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 579
BseBI CCWGG 3 cut(s) 246, 381, 485
BseDI CCNNGG 5 cut(s) 366, 411, 448, 1183, 1215
BseGI GGATG 4 cut(s) 228, 279, 595, 1040
BseLI CCNNNNNNNGG 3 cut(s) 238, 380, 1236
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 4 cut(s) 45, 128, 764, 1254
BseNI ACTGG 1 cut(s) 311
BseRI GAGGAG 1 cut(s) 740
BseXI GCAGC 2 cut(s) 388, 1003
BseYI CCCAGC 1 cut(s) 1269
BshFI GGCC 5 cut(s) 384, 453, 488, 513, 1172
BshNI GGYRCC 1 cut(s) 1252
BsiHKAI GWGCWC 1 cut(s) 48
BsiHKCI CYCGRG 1 cut(s) 827
BsiSI CCGG 4 cut(s) 489, 752, 795, 1083
BslFI GGGAC 4 cut(s) 21, 585, 921, 1252
BslI CCNNNNNNNGG 3 cut(s) 238, 380, 1236
BsmAI GTCTC 2 cut(s) 771, 830
BsmFI GGGAC 4 cut(s) 21, 585, 921, 1252
BsmI GAATGC 1 cut(s) 979
BsnI GGCC 5 cut(s) 384, 453, 488, 513, 1172
Bso31I GGTCTC 1 cut(s) 830
BsoBI CYCGRG 1 cut(s) 827
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 3 cut(s) 78, 472, 866
Bsp1720I GCTNAGC 1 cut(s) 1132
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 4 cut(s) 39, 185, 399, 531
BspANI GGCC 5 cut(s) 384, 453, 488, 513, 1172
BspCNI CTCAG 4 cut(s) 44, 127, 765, 1255
BspLI GGNNCC 6 cut(s) 37, 243, 372, 474, 937, 1254
BspPI GGATC 2 cut(s) 467, 480
BspT107I GGYRCC 1 cut(s) 1252
BspTNI GGTCTC 1 cut(s) 830
BsrDI GCAATG 1 cut(s) 579
BsrFI RCCGGY 2 cut(s) 488, 1082
BsrI ACTGG 1 cut(s) 311
BssAI RCCGGY 2 cut(s) 488, 1082
BssECI CCNNGG 5 cut(s) 366, 411, 448, 1183, 1215
BssMI GATC 3 cut(s) 78, 472, 866
BssNI GRCGYC 1 cut(s) 924
BssT1I CCWWGG 3 cut(s) 366, 411, 1215
Bst2UI CCWGG 3 cut(s) 246, 381, 485
Bst4CI ACNGT 3 cut(s) 22, 699, 803
BstACI GRCGYC 1 cut(s) 924
BstAPI GCANNNNNTGC 1 cut(s) 1279
BstDEI CTNAG 9 cut(s) 31, 114, 756, 773, 955, 968, 990, 1132, 1263
BstDSI CCRYGG 2 cut(s) 366, 1183
BstEII GGTNACC 1 cut(s) 187
BstENI CCTNNNNNAGG 1 cut(s) 378
BstF5I GGATG 4 cut(s) 228, 279, 595, 1040
BstKTI GATC 3 cut(s) 81, 475, 869
BstMAI GTCTC 2 cut(s) 771, 830
BstMBI GATC 3 cut(s) 78, 472, 866
BstMWI GCNNNNNNNGC 5 cut(s) 407, 502, 677, 815, 1279
BstNI CCWGG 3 cut(s) 246, 381, 485
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 4 cut(s) 244, 379, 483, 793
BstSFI CTRYAG 1 cut(s) 1039
BstV1I GCAGC 2 cut(s) 388, 1003
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 3 cut(s) 78, 472, 866
BstYI RGATCY 3 cut(s) 78, 472, 866
Bsu36I CCTNAGG 1 cut(s) 31
BsuRI GGCC 5 cut(s) 384, 453, 488, 513, 1172
BtgI CCRYGG 2 cut(s) 366, 1183
BtgZI GCGATG 1 cut(s) 1101
BtrI CACGTC 1 cut(s) 1285
BtsCI GGATG 4 cut(s) 228, 279, 595, 1040
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 2 cut(s) 128, 695
CaiI CAGNNNCTG 1 cut(s) 772
Cfr10I RCCGGY 2 cut(s) 488, 1082
Cfr13I GGNCC 2 cut(s) 35, 935
CseI GACGC 1 cut(s) 636
Csp6I GTAC 2 cut(s) 361, 760
CviAII CATG 2 cut(s) 367, 1020
CviQI GTAC 2 cut(s) 361, 760
DdeI CTNAG 9 cut(s) 31, 114, 756, 773, 955, 968, 990, 1132, 1263
DpnI GATC 3 cut(s) 80, 474, 868
DpnII GATC 3 cut(s) 78, 472, 866
DraIII CACNNNGTG 1 cut(s) 460
EaeI YGGCCR 1 cut(s) 511
Ecl136II GAGCTC 1 cut(s) 46
Eco130I CCWWGG 3 cut(s) 366, 411, 1215
Eco147I AGGCCT 1 cut(s) 1172
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 830
Eco47I GGWCC 2 cut(s) 35, 935
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 749, 1014
Eco81I CCTNAGG 1 cut(s) 31
Eco88I CYCGRG 1 cut(s) 827
Eco91I GGTNACC 1 cut(s) 187
EcoICRI GAGCTC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 35
EcoO65I GGTNACC 1 cut(s) 187
EcoRI GAATTC 2 cut(s) 127, 177
EcoRII CCWGG 3 cut(s) 244, 379, 483
EcoT14I CCWWGG 3 cut(s) 366, 411, 1215
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 366, 411, 1215
FaeI CATG 2 cut(s) 370, 1023
FaiI YATR 7 cut(s) 368, 557, 782, 859, 863, 952, 1021
FalI AAGNNNNNCTT 1 cut(s) 31
FaqI GGGAC 4 cut(s) 21, 585, 921, 1252
FatI CATG 2 cut(s) 366, 1019
FauI CCCGC 1 cut(s) 46
FblI GTMKAC 2 cut(s) 1095, 1221
Fnu4HI GCNGC 3 cut(s) 402, 531, 1017
FokI GGATG 4 cut(s) 215, 266, 602, 1047
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 3 cut(s) 402, 531, 1017
FspBI CTAG 2 cut(s) 209, 672
GluI GCNGC 3 cut(s) 402, 531, 1017
GsaI CCCAGC 1 cut(s) 1273
GsuI CTGGAG 2 cut(s) 228, 363
HaeIII GGCC 5 cut(s) 384, 453, 488, 513, 1172
HapII CCGG 4 cut(s) 489, 752, 795, 1083
HgaI GACGC 1 cut(s) 636
Hin1I GRCGYC 1 cut(s) 924
Hin1II CATG 2 cut(s) 370, 1023
HincII GTYRAC 3 cut(s) 271, 1096, 1222
HindII GTYRAC 3 cut(s) 271, 1096, 1222
HinfI GANTC 8 cut(s) 743, 748, 830, 844, 964, 994, 1223, 1265
HpaII CCGG 4 cut(s) 489, 752, 795, 1083
HphI GGTGA 4 cut(s) 181, 277, 977, 1202
Hpy166II GTNNAC 5 cut(s) 271, 312, 363, 1096, 1222
Hpy188I TCNGA 4 cut(s) 117, 449, 526, 768
Hpy188III TCNNGA 7 cut(s) 55, 476, 703, 827, 841, 1032, 1069
Hpy8I GTNNAC 5 cut(s) 271, 312, 363, 1096, 1222
Hpy99I CGWCG 2 cut(s) 929, 1150
HpyAV CCTTC 6 cut(s) 148, 265, 384, 773, 1038, 1240
HpyCH4III ACNGT 3 cut(s) 22, 699, 803
HpyCH4IV ACGT 3 cut(s) 338, 924, 1284
HpyF10VI GCNNNNNNNGC 5 cut(s) 407, 502, 677, 815, 1279
HpyF3I CTNAG 9 cut(s) 31, 114, 756, 773, 955, 968, 990, 1132, 1263
HpySE526I ACGT 3 cut(s) 338, 924, 1284
Hsp92I GRCGYC 1 cut(s) 924
Hsp92II CATG 2 cut(s) 370, 1023
Kzo9I GATC 3 cut(s) 78, 472, 866
LmnI GCTCC 2 cut(s) 247, 370
Lsp1109I GCAGC 2 cut(s) 388, 1003
LweI GCATC 5 cut(s) 102, 397, 517, 956, 1025
MaeI CTAG 2 cut(s) 209, 672
MaeII ACGT 3 cut(s) 338, 924, 1284
MaeIII GTNAC 4 cut(s) 187, 331, 797, 831
MalI GATC 3 cut(s) 80, 474, 868
MboI GATC 3 cut(s) 78, 472, 866
MboII GAAGA 2 cut(s) 356, 1156
MfeI CAATTG 2 cut(s) 664, 1277
MflI RGATCY 3 cut(s) 78, 472, 866
MhlI GDGCHC 1 cut(s) 48
MlsI TGGCCA 1 cut(s) 513
MluNI TGGCCA 1 cut(s) 513
MlyI GAGTC 4 cut(s) 838, 839, 1217, 1274
MmeI TCCRAC 1 cut(s) 256
Mox20I TGGCCA 1 cut(s) 513
MscI TGGCCA 1 cut(s) 513
MseI TTAA 4 cut(s) 17, 498, 615, 1293
Msp20I TGGCCA 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 4 cut(s) 489, 752, 795, 1083
MspR9I CCNGG 4 cut(s) 246, 381, 485, 795
MunI CAATTG 2 cut(s) 664, 1277
Mva1269I GAATGC 1 cut(s) 979
MvaI CCWGG 3 cut(s) 246, 381, 485
MwoI GCNNNNNNNGC 5 cut(s) 407, 502, 677, 815, 1279
NciI CCSGG 1 cut(s) 795
NcoI CCATGG 1 cut(s) 366
NdeII GATC 3 cut(s) 78, 472, 866
NlaIII CATG 2 cut(s) 370, 1023
NlaIV GGNNCC 6 cut(s) 37, 243, 372, 474, 937, 1254
NmeAIII GCCGAG 1 cut(s) 67
NmuCI GTSAC 3 cut(s) 187, 331, 831
PaeR7I CTCGAG 1 cut(s) 827
PceI AGGCCT 1 cut(s) 1172
PcsI WCGNNNNNNNCGW 1 cut(s) 1191
PctI GAATGC 1 cut(s) 979
PfeI GAWTC 4 cut(s) 743, 748, 964, 994
PkrI GCNGC 3 cut(s) 403, 532, 1018
PleI GAGTC 4 cut(s) 838, 838, 1217, 1273
PpsI GAGTC 4 cut(s) 838, 838, 1217, 1273
PpuMI RGGWCCY 1 cut(s) 35
Psp124BI GAGCTC 1 cut(s) 48
Psp1406I AACGTT 1 cut(s) 338
Psp5II RGGWCCY 1 cut(s) 35
Psp6I CCWGG 3 cut(s) 244, 379, 483
PspEI GGTNACC 1 cut(s) 187
PspFI CCCAGC 1 cut(s) 1269
PspGI CCWGG 3 cut(s) 244, 379, 483
PspN4I GGNNCC 6 cut(s) 37, 243, 372, 474, 937, 1254
PspPI GGNCC 2 cut(s) 35, 935
PspPPI RGGWCCY 1 cut(s) 35
PstNI CAGNNNCTG 1 cut(s) 772
PsuI RGATCY 3 cut(s) 78, 472, 866
RsaI GTAC 2 cut(s) 362, 761
RsaNI GTAC 2 cut(s) 361, 760
SacI GAGCTC 1 cut(s) 48
SalI GTCGAC 2 cut(s) 1094, 1220
SaqAI TTAA 4 cut(s) 17, 498, 615, 1293
SatI GCNGC 3 cut(s) 402, 531, 1017
Sau3AI GATC 3 cut(s) 78, 472, 866
Sau96I GGNCC 2 cut(s) 35, 935
SchI GAGTC 4 cut(s) 838, 839, 1217, 1274
ScrFI CCNGG 4 cut(s) 246, 381, 485, 795
SduI GDGCHC 1 cut(s) 48
SfaNI GCATC 5 cut(s) 102, 397, 517, 956, 1025
SfcI CTRYAG 1 cut(s) 1039
Sfr274I CTCGAG 1 cut(s) 827
SgrAI CRCCGGYG 1 cut(s) 1082
SinI GGWCC 2 cut(s) 35, 935
SlaI CTCGAG 1 cut(s) 827
SmlI CTYRAG 2 cut(s) 5, 827
SmoI CTYRAG 2 cut(s) 5, 827
SseBI AGGCCT 1 cut(s) 1172
SsiI CCGC 4 cut(s) 39, 185, 399, 531
SspMI CTAG 2 cut(s) 209, 672
SstI GAGCTC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 1172
StyD4I CCNGG 4 cut(s) 244, 379, 483, 793
StyI CCWWGG 3 cut(s) 366, 411, 1215
TaaI ACNGT 3 cut(s) 22, 699, 803
TaiI ACGT 3 cut(s) 341, 927, 1287
TaqI TCGA 6 cut(s) 54, 746, 828, 1095, 1167, 1221
TauI GCSGC 1 cut(s) 533
TfiI GAWTC 4 cut(s) 743, 748, 964, 994
Tru1I TTAA 4 cut(s) 17, 498, 615, 1293
Tru9I TTAA 4 cut(s) 17, 498, 615, 1293
TscAI CASTG 2 cut(s) 128, 702
TseFI GTSAC 3 cut(s) 187, 331, 831
TseI GCWGC 2 cut(s) 401, 1016
Tsp45I GTSAC 3 cut(s) 187, 331, 831
TspDTI ATGAA 1 cut(s) 368
TspGWI ACGGA 1 cut(s) 1070
TspRI CASTG 2 cut(s) 128, 702
VpaK11BI GGWCC 2 cut(s) 35, 935
XagI CCTNNNNNAGG 1 cut(s) 378
XapI RAATTY 4 cut(s) 127, 177, 604, 656
XhoI CTCGAG 1 cut(s) 827
XmiI GTMKAC 2 cut(s) 1095, 1221
XspI CTAG 2 cut(s) 209, 672
ZraI GACGTC 1 cut(s) 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.