MD05G1260600.v1.1

Brf1-like TBP-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001593
Physical Location & Seq
Reverse (-)
39572397 .. 39575265
2869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1260600.v1.1.491

Sequence Viewer

Length: 354 bp
ATGGATATGCTTCGTTTAAGTATTATTCCTCCAGAGAGGCTGAGTTCAAAAATCGACTTTGATGCTTTGAAGAAATTAAATGAAAAGCTTGGACCTGGATTTGAAGAACGCAAAGAGTCTAATTGCGGCAGTGGTAAACAGCTGTCAAATCAGAAAAAGATTCATTTTGAAGTTAGTCCTGATGAGGAGCTCGAACATGAAAATACGTACAGTGAAGAGGCTGAAGCCGGAGAAGGCTATGACGATGATACACATGTTGCAAACGACGAATATAATGGATGCAATTATGATGAAGTATACAATTACGATGAGGGATATAATAACGACGATGGATATGATTTTGACGAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRF1

Protein Analysis

118

Amino Acids

13.47

Weight (kDa)

4.17

Isoelectric Point (pI)

29.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 297
AciI CCGC 1 cut(s) 126
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 1 cut(s) 209
AflIII ACRYGT 1 cut(s) 253
AgsI TTSAA 4 cut(s) 48, 70, 104, 170
AjnI CCWGG 1 cut(s) 94
AluBI AGCT 3 cut(s) 88, 142, 190
AluI AGCT 3 cut(s) 88, 142, 190
Alw21I GWGCWC 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 92
AvaII GGWCC 1 cut(s) 92
BaeI ACNNNNGTAYC 2 cut(s) 240, 273
BanII GRGCYC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 192
BccI CCATC 1 cut(s) 323
BcgI CGANNNNNNTGC 2 cut(s) 44, 78
BciT130I CCWGG 1 cut(s) 96
BisI GCNGC 1 cut(s) 127
BlsI GCNGC 1 cut(s) 128
Bme1390I CCNGG 1 cut(s) 96
Bme18I GGWCC 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 92
BmrFI CCNGG 1 cut(s) 96
BmsI GCATC 2 cut(s) 52, 269
BpmI CTGGAG 1 cut(s) 15
BsaAI YACGTR 1 cut(s) 207
BseBI CCWGG 1 cut(s) 96
BseGI GGATG 1 cut(s) 284
BseMII CTCAG 1 cut(s) 32
BseRI GAGGAG 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 1 cut(s) 228
Bsp1286I GDGCHC 1 cut(s) 192
BspACI CCGC 1 cut(s) 126
BspCNI CTCAG 1 cut(s) 33
BssNAI GTATAC 1 cut(s) 298
Bst1107I GTATAC 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 212
Bst6I CTCTTC 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 207
BstDEI CTNAG 1 cut(s) 41
BstF5I GGATG 1 cut(s) 284
BstNI CCWGG 1 cut(s) 96
BstNSI RCATGY 1 cut(s) 257
BstSCI CCNGG 1 cut(s) 94
BstSNI TACGTA 1 cut(s) 207
BstZ17I GTATAC 1 cut(s) 298
BtsCI GGATG 1 cut(s) 284
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 136, 217
Cfr13I GGNCC 1 cut(s) 92
Csp6I GTAC 1 cut(s) 208
CviAII CATG 2 cut(s) 197, 254
CviJI RGCY 7 cut(s) 40, 88, 142, 190, 221, 227, 237
CviKI_1 RGCY 7 cut(s) 40, 88, 142, 190, 221, 227, 237
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 190
Eco105I TACGTA 1 cut(s) 207
Eco24I GRGCYC 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 92
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 243
EcoICRI GAGCTC 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 2 cut(s) 200, 257
FaiI YATR 9 cut(s) 8, 198, 240, 255, 273, 288, 298, 318, 336
FatI CATG 2 cut(s) 196, 253
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 1 cut(s) 127
FokI GGATG 1 cut(s) 291
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 1 cut(s) 127
GluI GCNGC 1 cut(s) 127
GsuI CTGGAG 1 cut(s) 15
HapII CCGG 1 cut(s) 228
Hin1II CATG 2 cut(s) 200, 257
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 2 cut(s) 116, 160
HpaII CCGG 1 cut(s) 228
Hpy166II GTNNAC 2 cut(s) 137, 298
Hpy188I TCNGA 1 cut(s) 153
Hpy188III TCNNGA 2 cut(s) 32, 179
Hpy8I GTNNAC 2 cut(s) 137, 298
Hpy99I CGWCG 2 cut(s) 269, 329
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 1 cut(s) 212
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 2 cut(s) 260, 282
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 1 cut(s) 206
Hsp92II CATG 2 cut(s) 200, 257
LmnI GCTCC 1 cut(s) 187
LpnPI CCDG 5 cut(s) 45, 81, 108, 192, 241
LweI GCATC 2 cut(s) 52, 269
MaeII ACGT 1 cut(s) 206
MboII GAAGA 3 cut(s) 82, 116, 227
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 5 cut(s) 74, 121, 283, 301, 347
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 5 cut(s) 30, 39, 178, 211, 304
MseI TTAA 2 cut(s) 17, 77
MspA1I CMGCKG 1 cut(s) 142
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 1 cut(s) 96
MvaI CCWGG 1 cut(s) 96
NlaIII CATG 2 cut(s) 200, 257
NspI RCATGY 1 cut(s) 257
PciI ACATGT 1 cut(s) 253
PfeI GAWTC 1 cut(s) 160
PkrI GCNGC 1 cut(s) 128
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Ppu21I YACGTR 1 cut(s) 207
PscI ACATGT 1 cut(s) 253
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 94
PspGI CCWGG 1 cut(s) 94
PspPI GGNCC 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 142
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 2 cut(s) 17, 77
SatI GCNGC 1 cut(s) 127
Sau96I GGNCC 1 cut(s) 92
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 1 cut(s) 192
SetI ASST 5 cut(s) 90, 97, 144, 192, 209
SfaNI GCATC 2 cut(s) 52, 269
SgeI CNNG 9 cut(s) 44, 101, 107, 108, 191, 203, 209, 240, 266
SinI GGWCC 1 cut(s) 92
SnaBI TACGTA 1 cut(s) 207
Sse9I AATT 5 cut(s) 74, 121, 283, 301, 347
SsiI CCGC 1 cut(s) 126
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 1 cut(s) 212
TaiI ACGT 1 cut(s) 209
TaqI TCGA 2 cut(s) 54, 192
TasI AATT 5 cut(s) 74, 121, 283, 301, 347
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 2 cut(s) 17, 77
Tru9I TTAA 2 cut(s) 17, 77
TscAI CASTG 2 cut(s) 136, 217
TspDTI ATGAA 4 cut(s) 96, 152, 213, 306
TspRI CASTG 2 cut(s) 136, 217
VpaK11BI GGWCC 1 cut(s) 92
XceI RCATGY 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.