Rh6DG420400

Brf1-like TBP-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001593
Physical Location & Seq
Forward (+)
60270869 .. 60271426
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG420400.1

Sequence Viewer

Length: 558 bp
ATGGCATTCCGAAAGCGATCCAAGTCGAGGCAAGAGCAGAAAGCTGATGAGGGTAATGGTTTTGATGAGGGCTCAAGTACATCAGACTCGGATAGTAGTGATAATGTCTCGGAAAGTCTTTCTGATATTGACGATGCCGAGATTTCTCCATACCTACTAACCAAGAAGGAGGCACTGTATAAAACCATCATCTGGGAAGCAATGCACAAGGATTATGTCGAGAAAAGAAGTACTAGAAAAAGAGCCAGAAAAGCCAAGGAAGCTGGTCCTCGGAGAAAGGCAGCCAAAACTTCTACCAAGAAATCGGATCTTGCACCGGAAGTCAAAAAAGGACGGAGTTCGAAAATCAACTATAATGCCATAAATGAAGAGGATTGCAGGTTGGAAGAAGGTTTGGAGTCTAACGAGAAAGTCATCGGTGCAGAGGCTCAAGACGGACAAGGCTATTATGATGATAATTATGAGTCTGAGCATGAAAACCAGTACAATGAAGAGGACGAGTCTTATTATGCAAATGATGATGGTGATGATAACGGGCACAATGATGGATGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRF1

Protein Analysis

185

Amino Acids

21.05

Weight (kDa)

4.8

Isoelectric Point (pI)

59.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRF1 PF07741 41 - 122 2.4e-10 Brf1-like TBP-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 369
AccB7I CCANNNNNTGG 1 cut(s) 192
AclWI GGATC 2 cut(s) 12, 315
AfaI GTAC 3 cut(s) 79, 232, 485
AfiI CCNNNNNNNGG 2 cut(s) 27, 192
AluBI AGCT 2 cut(s) 44, 263
AluI AGCT 2 cut(s) 44, 263
Alw26I GTCTC 1 cut(s) 112
AlwI GGATC 2 cut(s) 12, 315
ApeKI GCWGC 1 cut(s) 281
AspS9I GGNCC 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 536
AsuII TTCGAA 1 cut(s) 341
AvaII GGWCC 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 540
BanII GRGCYC 1 cut(s) 74
BbvI GCAGC 1 cut(s) 293
BccI CCATC 4 cut(s) 194, 515, 539, 543
BcoDI GTCTC 1 cut(s) 112
BfaI CTAG 1 cut(s) 234
BfuAI ACCTGC 1 cut(s) 369
BisI GCNGC 1 cut(s) 282
BlsI GCNGC 1 cut(s) 283
BmcAI AGTACT 1 cut(s) 232
Bme18I GGWCC 1 cut(s) 266
BmgT120I GGNCC 1 cut(s) 266
BmsI GCATC 1 cut(s) 124
Bpu14I TTCGAA 1 cut(s) 341
BpuEI CTTGAG 2 cut(s) 58, 414
BsaJI CCNNGG 2 cut(s) 255, 269
BsaWI WCCGGW 1 cut(s) 316
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 2 cut(s) 27, 192
Bse1I ACTGG 1 cut(s) 481
Bse3DI GCAATG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 255, 269
BseGI GGATG 1 cut(s) 554
BseLI CCNNNNNNNGG 2 cut(s) 27, 192
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 459
BseNI ACTGG 1 cut(s) 481
BseSI GKGCMC 1 cut(s) 540
BseXI GCAGC 1 cut(s) 293
BsgI GTGCAG 1 cut(s) 441
BsiSI CCGG 1 cut(s) 317
BslI CCNNNNNNNGG 2 cut(s) 27, 192
BsmAI GTCTC 1 cut(s) 112
BsmI GAATGC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 341
Bsp1286I GDGCHC 2 cut(s) 74, 540
Bsp143I GATC 2 cut(s) 17, 307
BspCNI CTCAG 1 cut(s) 460
BspMI ACCTGC 1 cut(s) 369
BspPI GGATC 2 cut(s) 12, 315
BspT104I TTCGAA 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 207
BsrI ACTGG 1 cut(s) 481
BssECI CCNNGG 2 cut(s) 255, 269
BssMI GATC 2 cut(s) 17, 307
BssT1I CCWWGG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 177
Bst6I CTCTTC 2 cut(s) 363, 486
BstBI TTCGAA 1 cut(s) 341
BstDEI CTNAG 1 cut(s) 468
BstF5I GGATG 1 cut(s) 554
BstKTI GATC 2 cut(s) 20, 310
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 2 cut(s) 17, 307
BstMWI GCNNNNNNNGC 2 cut(s) 251, 260
BstSLI GKGCMC 1 cut(s) 540
BstV1I GCAGC 1 cut(s) 293
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BtsCI GGATG 1 cut(s) 554
BtsIMutI CAGTG 1 cut(s) 173
BveI ACCTGC 1 cut(s) 369
Cfr13I GGNCC 1 cut(s) 266
Csp6I GTAC 3 cut(s) 78, 231, 484
CviAII CATG 1 cut(s) 473
CviJI RGCY 8 cut(s) 44, 72, 245, 254, 263, 284, 428, 444
CviKI_1 RGCY 8 cut(s) 44, 72, 245, 254, 263, 284, 428, 444
CviQI GTAC 3 cut(s) 78, 231, 484
DdeI CTNAG 1 cut(s) 468
DpnI GATC 2 cut(s) 19, 309
DpnII GATC 2 cut(s) 17, 307
Eam1104I CTCTTC 2 cut(s) 363, 486
EarI CTCTTC 2 cut(s) 363, 486
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 74
Eco47I GGWCC 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 74
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 1 cut(s) 476
FaiI YATR 9 cut(s) 151, 180, 216, 354, 362, 450, 462, 474, 510
FatI CATG 1 cut(s) 472
Fnu4HI GCNGC 1 cut(s) 282
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 1 cut(s) 282
FspBI CTAG 1 cut(s) 234
GluI GCNGC 1 cut(s) 282
HapII CCGG 1 cut(s) 317
Hin1II CATG 1 cut(s) 476
HinfI GANTC 4 cut(s) 86, 398, 464, 500
HpaII CCGG 1 cut(s) 317
HphI GGTGA 1 cut(s) 536
Hpy188I TCNGA 9 cut(s) 11, 85, 91, 112, 124, 273, 307, 469, 557
Hpy188III TCNNGA 2 cut(s) 220, 431
HpyAV CCTTC 2 cut(s) 160, 383
HpyCH4III ACNGT 1 cut(s) 177
HpyCH4V TGCA 5 cut(s) 205, 314, 378, 422, 512
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 260
HpyF3I CTNAG 1 cut(s) 468
Hsp92II CATG 1 cut(s) 476
Kzo9I GATC 2 cut(s) 17, 307
LpnPI CCDG 6 cut(s) 178, 249, 259, 330, 364, 494
Lsp1109I GCAGC 1 cut(s) 293
LweI GCATC 1 cut(s) 124
MaeI CTAG 1 cut(s) 234
MalI GATC 2 cut(s) 19, 309
MboI GATC 2 cut(s) 17, 307
MboII GAAGA 3 cut(s) 380, 398, 503
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 2 cut(s) 74, 540
MluCI AATT 1 cut(s) 457
MlyI GAGTC 4 cut(s) 80, 407, 473, 509
MmeI TCCRAC 1 cut(s) 363
MnlI CCTC 8 cut(s) 21, 43, 61, 163, 279, 364, 418, 487
MslI CAYNNNNRTG 1 cut(s) 543
MspI CCGG 1 cut(s) 317
Mva1269I GAATGC 1 cut(s) 5
MwoI GCNNNNNNNGC 2 cut(s) 251, 260
NdeII GATC 2 cut(s) 17, 307
NlaIII CATG 1 cut(s) 476
NmeAIII GCCGAG 1 cut(s) 163
NspV TTCGAA 1 cut(s) 341
PctI GAATGC 1 cut(s) 5
PflMI CCANNNNNTGG 1 cut(s) 192
PkrI GCNGC 1 cut(s) 283
PleI GAGTC 4 cut(s) 80, 406, 472, 508
PpsI GAGTC 4 cut(s) 80, 406, 472, 508
PspPI GGNCC 1 cut(s) 266
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 3 cut(s) 79, 232, 485
RsaNI GTAC 3 cut(s) 78, 231, 484
RseI CAYNNNNRTG 1 cut(s) 543
SatI GCNGC 1 cut(s) 282
Sau3AI GATC 2 cut(s) 17, 307
Sau96I GGNCC 1 cut(s) 266
ScaI AGTACT 1 cut(s) 232
SchI GAGTC 4 cut(s) 80, 407, 473, 509
SduI GDGCHC 2 cut(s) 74, 540
SetI ASST 5 cut(s) 46, 156, 265, 383, 394
SfaNI GCATC 1 cut(s) 124
SfuI TTCGAA 1 cut(s) 341
SinI GGWCC 1 cut(s) 266
SmiMI CAYNNNNRTG 1 cut(s) 543
SmlI CTYRAG 2 cut(s) 73, 429
SmoI CTYRAG 2 cut(s) 73, 429
Sse9I AATT 1 cut(s) 457
SspMI CTAG 1 cut(s) 234
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 1 cut(s) 177
TaqI TCGA 3 cut(s) 26, 219, 341
TasI AATT 1 cut(s) 457
TatI WGTACW 3 cut(s) 77, 230, 483
TscAI CASTG 1 cut(s) 180
TseI GCWGC 1 cut(s) 281
TspDTI ATGAA 3 cut(s) 381, 489, 504
TspGWI ACGGA 2 cut(s) 349, 450
TspRI CASTG 1 cut(s) 180
Van91I CCANNNNNTGG 1 cut(s) 192
VpaK11BI GGWCC 1 cut(s) 266
XspI CTAG 1 cut(s) 234
ZrmI AGTACT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.