Rorug05G0046200

Brf1-like TBP-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001593
Physical Location & Seq
Reverse (-)
4024838 .. 4027080
2243 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0046200.1

Sequence Viewer

Length: 1125 bp
ATGGCGTGGTTGGTGTCGTTGAAGATTCTGTTGATTTCGAGCGGCGTTGTGGCTTTGGCTTTGGCCATGCAGCTCTATGTTCCGTTGGTGTTTGAGTTCGCCGTCTCAAACCTTCCTCTGATCTGGAGCTCATGCAAATCCTTGCTCCGCCCGCCGTATCTCTACGTCGTCATCAACGCCATCATCATCACCATTGCCGCCTCCTCAATGTTCCACCATGACCGGCTTCAGGCAAAGCTTCCTTCCCCGGAGTCTGAATCAACGCCACCGCCGGTTGTATACAAGCACAGAGAAGATGACCTGGCGCCACTGGTGGTGTACAAGCATATACAAGAGGATGTGGCGCCACCAGTGGTGTACGAGCACACAGAAGATGACGTGGCGCCACTGGTGGTGTACAAGCATATACAAGAGGATGTGGCGCCACCAGTGTACGAGCACAGAAGAGAAGAGGATGTGGCGGCGTCACCGGTTGTGTACGATGACAGAGAGGAGGCCGTGATTAAGGAGGTGACGTCTATGGTACCATTTAACAACCCTGAAGAGCCTGTGGCTGAGGTTGATAATGATCGTGAGGATGAGATCGAGGTCTCGACAGCCACGTGGACGACGCCGGAGAATGCTACGAAATTCGATGATATTGTTTTGCCGGTGGAGAAGCCTCTAGTCTCTTCGAGATTCAGTCACCGGAAACCGGCTAGATCCACCCCTGAAGGTGGGAGGCCGTTACGAGTGTCCAAGGTGCCAAAACGGCACGACACGTTGGAGAACACGTGGAAGATGATAACGGAGGGGCGTGCGATGCCGCTGAGCCGGCACATCAAGAAGAGCGACACGTGGGATCATCACGGTAGCGGAGGTGTCAACGTTAACGATGAAGTGGACGAGGAGACGTCAGAAGCAGTGCAACAGTCAAAGACGTTTAAGGACATGACCGTGAGGACCATGGCAGCTGTGAGTTCCTCGCCGGGAGGGATTAAGCTGAGGAAAGAGCCGTCGCCGAGTCAGGACGATTTGAACCGCCGAGTCGAGGCGTTTATACAGAAGATCAACGAAGAAATGAGGTTGCAGAGACAGGAGTCATTGGATCAGTACATGGAGATGATCAACCGTGGAAGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRF1

Protein Analysis

374

Amino Acids

42.28

Weight (kDa)

5.26

Isoelectric Point (pI)

63.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 39 - 69 2.8e-12 Domain of unknown function (DUF4408)
DUF761 PF05553 336 - 371 2.3e-13 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 518, 896
Acc65I GGTACC 1 cut(s) 523
AccB1I GGYRCC 6 cut(s) 304, 343, 382, 421, 523, 742
AccBSI CCGCTC 1 cut(s) 42
AccI GTMKAC 1 cut(s) 279
AciI CCGC 9 cut(s) 42, 148, 152, 198, 269, 461, 806, 855, 1021
AclI AACGTT 1 cut(s) 867
AclWI GGATC 3 cut(s) 696, 849, 1095
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 629
AcuI CTGAAG 3 cut(s) 212, 561, 732
AcvI CACGTG 3 cut(s) 603, 774, 837
AcyI GRCGYC 8 cut(s) 305, 344, 383, 422, 464, 515, 611, 893
AfaI GTAC 7 cut(s) 320, 359, 398, 434, 479, 525, 1094
AfiI CCNNNNNNNGG 4 cut(s) 229, 694, 716, 1030
AflIII ACRYGT 3 cut(s) 759, 771, 834
AgeI ACCGGT 1 cut(s) 469
AgsI TTSAA 2 cut(s) 22, 1018
AjiI CACGTC 1 cut(s) 379
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 5 cut(s) 73, 129, 238, 953, 982
AluI AGCT 5 cut(s) 73, 129, 238, 953, 982
Alw21I GWGCWC 3 cut(s) 131, 366, 441
Alw26I GTCTC 5 cut(s) 109, 595, 673, 884, 1066
AlwI GGATC 3 cut(s) 696, 849, 1095
AoxI GGCC 3 cut(s) 63, 495, 722
ApeKI GCWGC 2 cut(s) 70, 950
ApoI RAATTY 1 cut(s) 629
AsiGI ACCGGT 1 cut(s) 469
Asp718I GGTACC 1 cut(s) 523
AspLEI GCGC 4 cut(s) 307, 346, 385, 424
AspS9I GGNCC 1 cut(s) 942
AsuC2I CCSGG 2 cut(s) 248, 969
AsuHPI GGTGA 4 cut(s) 181, 459, 523, 677
AvaII GGWCC 1 cut(s) 942
BalI TGGCCA 1 cut(s) 65
BanI GGYRCC 6 cut(s) 304, 343, 382, 421, 523, 742
BanII GRGCYC 1 cut(s) 131
BbrPI CACGTG 3 cut(s) 603, 774, 837
Bbv12I GWGCWC 3 cut(s) 131, 366, 441
BbvCI CCTCAGC 2 cut(s) 555, 983
BbvI GCAGC 2 cut(s) 82, 962
BccI CCATC 1 cut(s) 188
BceAI ACGGC 6 cut(s) 86, 139, 482, 709, 767, 979
BciT130I CCWGG 1 cut(s) 302
BclI TGATCA 1 cut(s) 1104
BcnI CCSGG 2 cut(s) 248, 969
BcoDI GTCTC 5 cut(s) 109, 595, 673, 884, 1066
BfaI CTAG 2 cut(s) 665, 699
BfoI RGCGCY 4 cut(s) 308, 347, 386, 425
BglI GCCNNNNNGGC 1 cut(s) 751
BisI GCNGC 6 cut(s) 43, 71, 198, 462, 806, 951
BlpI GCTNAGC 1 cut(s) 809
BlsI GCNGC 6 cut(s) 44, 72, 199, 463, 807, 952
Bme1390I CCNGG 3 cut(s) 248, 302, 969
Bme18I GGWCC 1 cut(s) 942
BmgBI CACGTC 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 942
BmiI GGNNCC 6 cut(s) 306, 345, 384, 423, 525, 744
BmrFI CCNGG 3 cut(s) 248, 302, 969
BmsI GCATC 1 cut(s) 792
BoxI GACNNNNGTC 1 cut(s) 1078
BpmI CTGGAG 1 cut(s) 145
Bpu10I CCTNAGC 2 cut(s) 555, 983
Bpu1102I GCTNAGC 1 cut(s) 809
BpuMI CCSGG 2 cut(s) 248, 969
BsaAI YACGTR 3 cut(s) 603, 774, 837
BsaBI GATNNNNATC 1 cut(s) 567
BsaHI GRCGYC 8 cut(s) 305, 344, 383, 422, 464, 515, 611, 893
BsaI GGTCTC 1 cut(s) 595
BsaJI CCNNGG 4 cut(s) 246, 738, 945, 1111
BsaWI WCCGGW 2 cut(s) 469, 687
BsaXI ACNNNNNCTCC 4 cut(s) 500, 530, 758, 788
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 694, 716, 1030
Bse118I RCCGGY 6 cut(s) 222, 271, 469, 649, 694, 813
Bse1I ACTGG 4 cut(s) 315, 350, 393, 428
Bse3DI GCAATG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 4 cut(s) 246, 738, 945, 1111
BseGI GGATG 4 cut(s) 343, 421, 460, 583
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 4 cut(s) 229, 694, 716, 1030
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 3 cut(s) 546, 800, 974
BseNI ACTGG 4 cut(s) 315, 350, 393, 428
BseRI GAGGAG 3 cut(s) 193, 506, 902
BseXI GCAGC 2 cut(s) 82, 962
BshFI GGCC 3 cut(s) 65, 497, 724
BshNI GGYRCC 6 cut(s) 304, 343, 382, 421, 523, 742
BshTI ACCGGT 1 cut(s) 469
BsiHKAI GWGCWC 3 cut(s) 131, 366, 441
BslI CCNNNNNNNGG 4 cut(s) 229, 694, 716, 1030
BsmAI GTCTC 5 cut(s) 109, 595, 673, 884, 1066
BsmBI CGTCTC 2 cut(s) 109, 884
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 3 cut(s) 65, 497, 724
Bso31I GGTCTC 1 cut(s) 595
Bsp1286I GDGCHC 3 cut(s) 131, 366, 441
Bsp1407I TGTACA 2 cut(s) 318, 396
Bsp143I GATC 8 cut(s) 120, 568, 582, 701, 841, 1047, 1087, 1104
Bsp1720I GCTNAGC 1 cut(s) 809
Bsp19I CCATGG 1 cut(s) 945
BspACI CCGC 9 cut(s) 42, 148, 152, 198, 269, 461, 806, 855, 1021
BspANI GGCC 3 cut(s) 65, 497, 724
BspCNI CTCAG 3 cut(s) 547, 801, 975
BspLI GGNNCC 6 cut(s) 306, 345, 384, 423, 525, 744
BspPI GGATC 3 cut(s) 696, 849, 1095
BspQI GCTCTTC 2 cut(s) 537, 821
BspT107I GGYRCC 6 cut(s) 304, 343, 382, 421, 523, 742
BspTNI GGTCTC 1 cut(s) 595
BsrBI CCGCTC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 6 cut(s) 222, 271, 469, 649, 694, 813
BsrGI TGTACA 2 cut(s) 318, 396
BsrI ACTGG 4 cut(s) 315, 350, 393, 428
BssAI RCCGGY 6 cut(s) 222, 271, 469, 649, 694, 813
BssECI CCNNGG 4 cut(s) 246, 738, 945, 1111
BssMI GATC 8 cut(s) 120, 568, 582, 701, 841, 1047, 1087, 1104
BssNAI GTATAC 1 cut(s) 280
BssNI GRCGYC 8 cut(s) 305, 344, 383, 422, 464, 515, 611, 893
BssT1I CCWWGG 2 cut(s) 738, 945
Bst1107I GTATAC 1 cut(s) 280
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 4 cut(s) 851, 912, 937, 1112
Bst6I CTCTTC 5 cut(s) 439, 444, 537, 676, 821
BstACI GRCGYC 8 cut(s) 305, 344, 383, 422, 464, 515, 611, 893
BstAUI TGTACA 2 cut(s) 318, 396
BstBAI YACGTR 3 cut(s) 603, 774, 837
BstC8I GCNNGC 3 cut(s) 152, 798, 815
BstDEI CTNAG 3 cut(s) 555, 809, 983
BstDSI CCRYGG 2 cut(s) 945, 1111
BstF5I GGATG 4 cut(s) 343, 421, 460, 583
BstH2I RGCGCY 4 cut(s) 308, 347, 386, 425
BstHHI GCGC 4 cut(s) 307, 346, 385, 424
BstKTI GATC 8 cut(s) 123, 571, 585, 704, 844, 1050, 1090, 1107
BstMAI GTCTC 5 cut(s) 109, 595, 673, 884, 1066
BstMBI GATC 8 cut(s) 120, 568, 582, 701, 841, 1047, 1087, 1104
BstMWI GCNNNNNNNGC 4 cut(s) 151, 751, 802, 814
BstNI CCWGG 1 cut(s) 302
BstPAI GACNNNNGTC 1 cut(s) 1078
BstSCI CCNGG 3 cut(s) 246, 300, 967
BstV1I GCAGC 2 cut(s) 82, 962
BstX2I RGATCY 1 cut(s) 701
BstYI RGATCY 1 cut(s) 701
BstZ17I GTATAC 1 cut(s) 280
BsuRI GGCC 3 cut(s) 65, 497, 724
BtgI CCRYGG 2 cut(s) 945, 1111
BtgZI GCGATG 1 cut(s) 815
BtrI CACGTC 1 cut(s) 379
BtsCI GGATG 4 cut(s) 343, 421, 460, 583
BtsI GCAGTG 1 cut(s) 909
BtsIMutI CAGTG 5 cut(s) 308, 357, 386, 435, 909
Cac8I GCNNGC 3 cut(s) 152, 798, 815
CfoI GCGC 4 cut(s) 307, 346, 385, 424
Cfr10I RCCGGY 6 cut(s) 222, 271, 469, 649, 694, 813
Cfr13I GGNCC 1 cut(s) 942
CseI GACGC 2 cut(s) 453, 619
Csp6I GTAC 7 cut(s) 319, 358, 397, 433, 478, 524, 1093
CspAI ACCGGT 1 cut(s) 469
CviAII CATG 6 cut(s) 67, 132, 218, 931, 946, 1096
CviQI GTAC 7 cut(s) 319, 358, 397, 433, 478, 524, 1093
DdeI CTNAG 3 cut(s) 555, 809, 983
DinI GGCGCC 4 cut(s) 306, 345, 384, 423
DpnI GATC 8 cut(s) 122, 570, 584, 703, 843, 1049, 1089, 1106
DpnII GATC 8 cut(s) 120, 568, 582, 701, 841, 1047, 1087, 1104
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 5 cut(s) 439, 444, 537, 676, 821
EarI CTCTTC 5 cut(s) 439, 444, 537, 676, 821
EciI GGCGGA 1 cut(s) 137
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 738, 945
Eco24I GRGCYC 1 cut(s) 131
Eco31I GGTCTC 1 cut(s) 595
Eco47I GGWCC 1 cut(s) 942
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 3 cut(s) 212, 561, 732
Eco72I CACGTG 3 cut(s) 603, 774, 837
EcoICRI GAGCTC 1 cut(s) 129
EcoRII CCWGG 1 cut(s) 300
EcoT14I CCWWGG 2 cut(s) 738, 945
EcoT38I GRGCYC 1 cut(s) 131
EgeI GGCGCC 4 cut(s) 306, 345, 384, 423
EheI GGCGCC 4 cut(s) 306, 345, 384, 423
ErhI CCWWGG 2 cut(s) 738, 945
Esp3I CGTCTC 2 cut(s) 109, 884
FaeI CATG 6 cut(s) 70, 135, 221, 934, 949, 1099
FatI CATG 6 cut(s) 66, 131, 217, 930, 945, 1095
FauI CCCGC 1 cut(s) 159
FbaI TGATCA 1 cut(s) 1104
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 6 cut(s) 43, 71, 198, 462, 806, 951
FokI GGATG 4 cut(s) 350, 428, 467, 590
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 6 cut(s) 43, 71, 198, 462, 806, 951
FspBI CTAG 2 cut(s) 665, 699
GlaI GCGC 4 cut(s) 306, 345, 384, 423
GluI GCNGC 6 cut(s) 43, 71, 198, 462, 806, 951
GsuI CTGGAG 1 cut(s) 145
HaeII RGCGCY 4 cut(s) 308, 347, 386, 425
HaeIII GGCC 3 cut(s) 65, 497, 724
HgaI GACGC 2 cut(s) 453, 619
HhaI GCGC 4 cut(s) 307, 346, 385, 424
Hin1I GRCGYC 8 cut(s) 305, 344, 383, 422, 464, 515, 611, 893
Hin1II CATG 6 cut(s) 70, 135, 221, 934, 949, 1099
Hin6I GCGC 4 cut(s) 305, 344, 383, 422
HinP1I GCGC 4 cut(s) 305, 344, 383, 422
HincII GTYRAC 2 cut(s) 865, 871
HindII GTYRAC 2 cut(s) 865, 871
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 7 cut(s) 25, 251, 257, 678, 1003, 1026, 1079
HpaI GTTAAC 1 cut(s) 871
HphI GGTGA 4 cut(s) 181, 459, 523, 677
Hpy188I TCNGA 3 cut(s) 120, 256, 898
Hpy188III TCNNGA 6 cut(s) 124, 572, 592, 675, 823, 1007
Hpy99I CGWCG 3 cut(s) 170, 613, 1000
HpyAV CCTTC 3 cut(s) 122, 252, 707
HpyCH4III ACNGT 4 cut(s) 851, 912, 937, 1112
HpyCH4V TGCA 4 cut(s) 70, 135, 907, 1069
HpyF10VI GCNNNNNNNGC 4 cut(s) 151, 751, 802, 814
HpyF3I CTNAG 3 cut(s) 555, 809, 983
Hsp92I GRCGYC 8 cut(s) 305, 344, 383, 422, 464, 515, 611, 893
Hsp92II CATG 6 cut(s) 70, 135, 221, 934, 949, 1099
HspAI GCGC 4 cut(s) 305, 344, 383, 422
KasI GGCGCC 4 cut(s) 304, 343, 382, 421
KpnI GGTACC 1 cut(s) 527
KroI GCCGGC 1 cut(s) 813
KroNI GCCGGC 1 cut(s) 815
Ksp22I TGATCA 1 cut(s) 1104
KspAI GTTAAC 1 cut(s) 871
Kzo9I GATC 8 cut(s) 120, 568, 582, 701, 841, 1047, 1087, 1104
LguI GCTCTTC 2 cut(s) 537, 821
LmnI GCTCC 2 cut(s) 126, 150
Lsp1109I GCAGC 2 cut(s) 82, 962
LweI GCATC 1 cut(s) 792
MaeI CTAG 2 cut(s) 665, 699
MaeIII GTNAC 4 cut(s) 465, 511, 683, 726
MalI GATC 8 cut(s) 122, 570, 584, 703, 843, 1049, 1089, 1106
MbiI CCGCTC 1 cut(s) 42
MboI GATC 8 cut(s) 120, 568, 582, 701, 841, 1047, 1087, 1104
MflI RGATCY 1 cut(s) 701
MhlI GDGCHC 3 cut(s) 131, 366, 441
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 1 cut(s) 629
MluNI TGGCCA 1 cut(s) 65
Mly113I GGCGCC 4 cut(s) 305, 344, 383, 422
MlyI GAGTC 4 cut(s) 260, 1012, 1035, 1088
MmeI TCCRAC 1 cut(s) 744
Mox20I TGGCCA 1 cut(s) 65
MroNI GCCGGC 1 cut(s) 813
MscI TGGCCA 1 cut(s) 65
MseI TTAA 5 cut(s) 504, 531, 870, 924, 978
MslI CAYNNNNRTG 2 cut(s) 935, 1100
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 2 cut(s) 808, 953
MspR9I CCNGG 3 cut(s) 248, 302, 969
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 1 cut(s) 302
MwoI GCNNNNNNNGC 4 cut(s) 151, 751, 802, 814
NaeI GCCGGC 1 cut(s) 815
NarI GGCGCC 4 cut(s) 305, 344, 383, 422
NciI CCSGG 2 cut(s) 248, 969
NcoI CCATGG 1 cut(s) 945
NdeII GATC 8 cut(s) 120, 568, 582, 701, 841, 1047, 1087, 1104
NgoMIV GCCGGC 1 cut(s) 813
NlaIII CATG 6 cut(s) 70, 135, 221, 934, 949, 1099
NlaIV GGNNCC 6 cut(s) 306, 345, 384, 423, 525, 744
NmeAIII GCCGAG 2 cut(s) 1026, 1049
NmuCI GTSAC 3 cut(s) 465, 511, 683
PciSI GCTCTTC 2 cut(s) 537, 821
PcsI WCGNNNNNNNCGW 1 cut(s) 599
PctI GAATGC 1 cut(s) 625
PdiI GCCGGC 1 cut(s) 815
PfeI GAWTC 3 cut(s) 25, 257, 678
PinAI ACCGGT 1 cut(s) 469
PkrI GCNGC 6 cut(s) 44, 72, 199, 463, 807, 952
PleI GAGTC 4 cut(s) 259, 1011, 1034, 1087
PluTI GGCGCC 4 cut(s) 308, 347, 386, 425
PmaCI CACGTG 3 cut(s) 603, 774, 837
PmlI CACGTG 3 cut(s) 603, 774, 837
PpsI GAGTC 4 cut(s) 259, 1011, 1034, 1087
Ppu21I YACGTR 3 cut(s) 603, 774, 837
PshAI GACNNNNGTC 1 cut(s) 1078
Psp124BI GAGCTC 1 cut(s) 131
Psp1406I AACGTT 1 cut(s) 867
Psp6I CCWGG 1 cut(s) 300
PspCI CACGTG 3 cut(s) 603, 774, 837
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 6 cut(s) 306, 345, 384, 423, 525, 744
PspPI GGNCC 1 cut(s) 942
PsuI RGATCY 1 cut(s) 701
PvuII CAGCTG 1 cut(s) 953
RsaI GTAC 7 cut(s) 320, 359, 398, 434, 479, 525, 1094
RsaNI GTAC 7 cut(s) 319, 358, 397, 433, 478, 524, 1093
RseI CAYNNNNRTG 2 cut(s) 935, 1100
SacI GAGCTC 1 cut(s) 131
SapI GCTCTTC 2 cut(s) 537, 821
SaqAI TTAA 5 cut(s) 504, 531, 870, 924, 978
SatI GCNGC 6 cut(s) 43, 71, 198, 462, 806, 951
Sau3AI GATC 8 cut(s) 120, 568, 582, 701, 841, 1047, 1087, 1104
Sau96I GGNCC 1 cut(s) 942
SchI GAGTC 4 cut(s) 260, 1012, 1035, 1088
ScrFI CCNGG 3 cut(s) 248, 302, 969
SduI GDGCHC 3 cut(s) 131, 366, 441
SfaNI GCATC 1 cut(s) 792
SfoI GGCGCC 4 cut(s) 306, 345, 384, 423
SinI GGWCC 1 cut(s) 942
SmiMI CAYNNNNRTG 2 cut(s) 935, 1100
Sse9I AATT 1 cut(s) 629
SsiI CCGC 9 cut(s) 42, 148, 152, 198, 269, 461, 806, 855, 1021
SspDI GGCGCC 4 cut(s) 304, 343, 382, 421
SspMI CTAG 2 cut(s) 665, 699
SstI GAGCTC 1 cut(s) 131
StyD4I CCNGG 3 cut(s) 246, 300, 967
StyI CCWWGG 2 cut(s) 738, 945
TaaI ACNGT 4 cut(s) 851, 912, 937, 1112
TaqI TCGA 6 cut(s) 38, 585, 593, 633, 674, 1029
TasI AATT 1 cut(s) 629
TatI WGTACW 3 cut(s) 318, 396, 1092
TauI GCSGC 4 cut(s) 45, 200, 464, 808
TfiI GAWTC 3 cut(s) 25, 257, 678
Tru1I TTAA 5 cut(s) 504, 531, 870, 924, 978
Tru9I TTAA 5 cut(s) 504, 531, 870, 924, 978
TscAI CASTG 5 cut(s) 315, 357, 393, 435, 909
TseFI GTSAC 3 cut(s) 465, 511, 683
TseI GCWGC 2 cut(s) 70, 950
Tsp45I GTSAC 3 cut(s) 465, 511, 683
TspDTI ATGAA 1 cut(s) 891
TspGWI ACGGA 2 cut(s) 72, 803
TspRI CASTG 5 cut(s) 315, 357, 393, 435, 909
VpaK11BI GGWCC 1 cut(s) 942
XapI RAATTY 1 cut(s) 629
XmiI GTMKAC 1 cut(s) 279
XspI CTAG 2 cut(s) 665, 699
ZraI GACGTC 2 cut(s) 516, 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.