MD06G1059400.v1.1

Protein transport protein sec16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001182
Physical Location & Seq
Reverse (-)
9133207 .. 9141823
8617 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1059400.v1.1.491

Sequence Viewer

Length: 4254 bp
ATGGATTCAAATCCTCCTCCATTTCAGGTGGAGGATCAGGCGGACGAGGATTTCTTTGATAAATTGGTTGAAGGCGATCTGGGGCCCTCCGAATCAGGCTCCGAATTCGCCCAGGGAAATGATTCCGATGATGGTATGGCGTTCGCCAGTCTCAGTATTGGTGGTTCTGTAGCTGTATCTGAGGATTCGGGCCACAAAACCAAAACTATAGCCGAAAATAAGCCGTTTGCAAATCCGAATGTTGGTGATTCTGCAGCTGTATCTGAGGATTCGGTTGCCAAACCCCAATCGAAAGACGAAAATGGTGCAGACATGTCGAACAATGTGGTTAATAATGACGTAGTGATTGAGTCCAATAATGCTGGGGAGGGATCCCAATTGAGGCCAGATTCGACAGTGAGCAGGAGCAACGATTCGGGAGCTTCAGGGATTAAGGAGATAGGGTGGGGTTCATTTTATGCCGATTCTGCGGAGAATGGGATCCATGGATTTGGATCGTACTCGGATTTTTTCAATGAATTGGGGGATGGTTCTGGGGATATCCCCACAAAAGTAGACGAAAGTTTGAGTACTGAATTGAAAACTGTACGCAGCAATGAAGTTCAAACAGCCCATCAAGAGGGTTTGAACCATTTGGTTAATAACGAGCAATATCAAGAGGGTCAAGCTTATGGGGCAGCTGTGGAGGACAGCAGAAATGAGCAGGATCTAAACAGTACAGAGTATTGGGAAAGTCTCTATCCAGGGTGGAAGTATGATTCCAACACGGGGCAGTGGTATCAAGTGGACAGTTTCAATGTGCCTGGGAATGCTCAAGGGAGTTTTGGCACTGATGATTGGACCACTGTTTCAGATGATAATAAAACTGTGGTTTCTCATTTGCAGCAAACAGCTCAGTCTGTTGCAGGAACTGTGACTGAAACAAGCACAACTGGCAGTTTGTCTAACTGGGATCAGGTCTCACAAGGGACTAATGGGTACCCAGAACACATGGTTTTCAATCCTGAATACCCGGGTTGGTATTATGACACGATTGCCCAAGAATGGCGTTCATTGGAAGCATATAATTCCTCTGTGCAATCAACTGCTCAGTCTCAGAATGGAAATAGTATATATAGTCAAGAGTACAGGCAAGATGGCAATTATGGGTCACAAGCGGTCGTCAGGAACCAAGGTCAAGACAGCAGCTGGGCTGGATCTTACAGTAATTACAATCAGCAGGCTTCAAATATGTGGCAACCTCAAACTGCATCTAAAAGTGAGGGGTTTTCAGGTTTTAGTGGAAACCAGCAAATGAGTAATTCGTTTGGTTCTACAGTTAACACGGATCAGTACAAGTCTTTAAATTCTTTTGGATCAGTTCCTTTGTACAACAATGCAAGTCAAGGTCATGGTGAAGCTAATGGGACTGTTGTGTTCCAAAGTTTTGTGCCGGCTGGAAACTTTAGCCAGCAGTTTAATCAAGGAAATGCAAAGATGAGCGAACAAACACAATTCTCAGATGATTACTTTGGTGGTCAGAAACCTGTAAGTTATTCACAGCAACCTGTAAATTATTCACAACAACCTTTCCAGAGTGGCAATCAGTTTTCCTATGCTCCCAGCGTAGGAAGATCATCTGCTGGCCGTCCTCCACATGCCCTAGTAACTTTTGGATTCGGAGGAAAACTCATTGTAATGAGGGACAATAGTAGCCTCAGGAATCCGTCATATGGAACCCAGGATCCTGTAGGAGGCTCAGTTTCTGTCCTGAACTTGATGGAAGTTTTCACGGGAAAGACTGATGCTTCGAGTTTTGGAATGAGTACTTGTGATTATTTTCGTGCTTTGTGCCAACAATCCTTCCCAGGTCCCTTGGTTGGAGGGAGTGTTGGAAGCAAAGAGTTGAACAAATGGATTGATGAGAGGATTGCTAACTGTGAATTACCAGACATGGACTATAGGAAGGGCAAAGTATTGAGGTTGCTTCTTTCTTTGCTTAAGATAGCTTGTCAACATTATGGAAAACTTCGATCTCCTTTTGGTACCGACACTGTATCAAGGGAAAATGATACTCCAGAATCAGCCGTTGCTAAACTTTTTGCATCTGCAAAGAGCAATAATGTACAATTTAGTGATTATGGCACTGTTAGCCACTGTGTGCAGAAAATGCCTTCAGAAGGACAAATGCGGGCAACTGCTTCTGAGGTACAAAATTTTCTGGTTTCTGGTAGAAAGAAAGAGGCTTTACAGTGTGCACAAGGTGGTCAGTTGTGGGGACCTGCGCTTGTTATTGCTTCACAGCTTGGAGAACAGTTCTATGTGGACACTGTGAAGCAAATGGCACTCCGCCAGCTGGTTGCAGGATCACCTTTGCGGACTTTATGCCTGCTAATTGCAGGGCAACCTGCTGAAGTGTTCTCTGCTGATACTACAGCTGAGATCAATCTTCCAGGCACTGTAAGTACATCTCAACAGCCTGCACAGTTTGGTGCAAATAAAATGCTTGATGACTGGGAGGAGAATTTGGCTGTGATAACTGCAAACAGAACAAAAGATGATGAACTGGTAATTATACATCTGGGAGATTGTTTATGGAAGGATAGGAGTGAGATTACTGCAGCACACATCTGCTATTTAGTGGCAGAAGCAAATTTTGAGTCATACTCAGATAGTGCAAGGCTATGTCTAATTGGAGCAGATCACTGGAAGTCACCTCGTACTTATGCGAGTCCAGAAGCCATTCAGAGAACTGAACTCTATGAATATTCAAGGGTGCTTGGGAACTCTCAGTTCATCCTTCTACCATTTCAGCCATATAAGCTCATATATGCGCACATGCTTGCTGAAGTTGGAAGAGTTTCTGACTCGTTGAAGTACTGTCAAACAATACTAAAGTCTTTGAAAACTGGTAGAGCACCTGAAGTGGAAACGTGGAAGCAACTAGTATTATCTCTTGAGGAGAGGATTAAAACCCATCAGCAGGGGGGATACTCTGTAAATTTAGTTTCTACAAAGTTTGTTGGTAAATTGCTCAACCTGTTTGATAGTACTGCACATCGTGTTGTTGGCCTACCACCACCTGCACCATCCACATCCCAAGGAAGTGCTCACGGTAATGAACATTATCAACAGCCCACGGGCCCTAGAGTTTCTAGTAGTCAATCAACAATGGCCATGTCGTCGTTAATTCCTTCTGCTTCAATGGAACCCATAAGCGACTGGACATCTGATGGCAATAGAAAGCCAATGCACAATAGAAGTGTTTCAGAGCCAGATTTTGGTAGAACTCCAAGACAGGTTGATTCATCAAAGCAAACTGCCTCACCTGATGGCCAAGGAAAAGCCTCAGGGGGGACTTCTCGCTTTTCTCGTTTTGGTTTCGGGTCACAGCTGTTGCAAAAGACTGTTGGTTTAGTATTAAGACCCCGCCCGGGAAAACAGGCTAAGTTGGGTGAAACAAACAAGTTCTACTACGACGAAAAACTTAAAAGATGGGTTGAGGAAGGTGTTGAACCTCCAGCTGAAGAAGCTGCGTTGCCTCCTCCCCCGACAAGTACACCCTTTCAGAATGGTGTCTCTGATTACAACTTGAGATCTGTTGTGAAGAAAGAAGGGTCTCCTACTAAGGGCAGCCCAGATTTACAAACTTCAACCCCTCTAGGACCAACTTCAGGGACCCCACCTATTCCACCTAGCTCAAATCAATTTTCATCCCGTGCACGGTTGGGTATTCGATCCAGATACGTTGACACATTCAACCAAGGTGGTGGAAGCCCAGCAAACTTGTTCCAGTCACCTTCTGTTCCATCTGTTAAGCCTCCCGTTGCTGCCAATGCAAAGTTTTTTATTCCCACCTTAGCATCATCAAATGAACACGCAATGGAGGCTATTGCTGAGAGTGTACAAGAAGATGGTGCAACTAATGAAAATCTTTCAACATCTGGTATGAATGATTCATTCCATGCTCCACTGCCTTCATCGTCGTCATCAAATACCATGCAAAGGTTCCCAAGCATGGGTAATATTCAAAGCATGGGAGTTGCCACAAATGCCAATGACTCTGCTCTACCCCACTCACGAAGAACGGCTTCATGGGGTGGAAGCTCCAATGATGTATTAAGCCCTCATATGAAAACGGGCGAGATAAAACCTCTAGGGGAGGCATTAGGTATGTCCCCGGTGGCTATGTTTAGGCCGAGTGAGCCTTCCCTGACACGTGCACCAATGAATGGAGGCAGTTCTGGTGACGACCTTCATGAAGTGGAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec16 Sec16_C

Protein Analysis

1418

Amino Acids

153.16

Weight (kDa)

5.02

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec16 PF12932 546 - 668 2.8e-21 Vesicle coat trafficking protein Sec16 mid-region
TPR_Sec16 PF12931 730 - 990 1.3e-53 Sec16 Sec23-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3070
Acc16I TGCGCA 1 cut(s) 2814
Acc36I ACCTGC 3 cut(s) 2269, 2395, 3070
Acc65I GGTACC 2 cut(s) 978, 2024
AccB1I GGYRCC 2 cut(s) 978, 2024
AccB7I CCANNNNNTGG 2 cut(s) 3260, 4211
AccI GTMKAC 1 cut(s) 555
AciI CCGC 7 cut(s) 41, 470, 1157, 2170, 2329, 2356, 3409
AcoI YGGCCR 3 cut(s) 1624, 3153, 3313
AcsI RAATTY 6 cut(s) 104, 1345, 2194, 2503, 2632, 2980
AcuI CTGAAG 7 cut(s) 408, 2139, 2412, 2847, 2922, 3525, 3636
AcvI CACGTG 1 cut(s) 4199
AdeI CACNNNGTG 3 cut(s) 2140, 2243, 3041
AflII CTTAAG 1 cut(s) 1979
AflIII ACRYGT 2 cut(s) 312, 4196
AhlI ACTAGT 1 cut(s) 2923
AjnI CCWGG 6 cut(s) 111, 742, 802, 1719, 1846, 2431
AloI GAACNNNNNNTCC 2 cut(s) 1708, 1740
Alw21I GWGCWC 5 cut(s) 2239, 2899, 3091, 3703, 4204
Alw26I GTCTC 6 cut(s) 155, 740, 964, 1098, 3562, 3603
Alw44I GTGCAC 3 cut(s) 2235, 3699, 4200
AlwNI CAGNNNCTG 4 cut(s) 911, 1188, 1745, 2438
Ama87I CYCGRG 2 cut(s) 1012, 3412
AoxI GGCC 9 cut(s) 83, 190, 383, 1624, 3049, 3121, 3153, 3313, 4175
ApaI GGGCCC 2 cut(s) 87, 3125
ApaLI GTGCAC 3 cut(s) 2235, 3699, 4200
ApeKI GCWGC 9 cut(s) 254, 591, 677, 883, 1185, 2600, 3512, 3612, 3809
ApoI RAATTY 6 cut(s) 104, 1345, 2194, 2503, 2632, 2980
ArsI GACNNNNNNTTYG 4 cut(s) 1771, 1803, 2624, 2656
Asp700I GAANNNNTTC 5 cut(s) 121, 2721, 2839, 3244, 4069
Asp718I GGTACC 2 cut(s) 978, 2024
AspLEI GCGC 2 cut(s) 2266, 2815
AsuC2I CCSGG 5 cut(s) 1013, 1014, 3413, 3414, 4160
AsuHPI GGTGA 8 cut(s) 257, 1406, 2340, 2685, 3297, 3446, 3768, 4238
AvaI CYCGRG 2 cut(s) 1012, 3412
AvaII GGWCC 5 cut(s) 840, 1850, 2258, 3644, 3657
AxyI CCTNAGG 2 cut(s) 1697, 3328
BaeGI GKGCMC 5 cut(s) 87, 2239, 3125, 3703, 4204
BalI TGGCCA 2 cut(s) 3155, 3315
BamHI GGATCC 3 cut(s) 371, 480, 1723
BanI GGYRCC 2 cut(s) 978, 2024
BanII GRGCYC 2 cut(s) 87, 3125
BbrPI CACGTG 1 cut(s) 4199
Bbv12I GWGCWC 5 cut(s) 2239, 2899, 3091, 3703, 4204
BbvI GCAGC 9 cut(s) 266, 603, 689, 895, 1197, 2612, 3499, 3624, 3796
BceAI ACGGC 4 cut(s) 208, 1611, 2051, 4083
BcgI CGANNNNNNTGC 4 cut(s) 287, 297, 321, 331
BciT130I CCWGG 6 cut(s) 113, 744, 804, 1721, 1848, 2433
BciVI GTATCC 1 cut(s) 2963
BcnI CCSGG 5 cut(s) 1013, 1014, 3413, 3414, 4160
BcoDI GTCTC 6 cut(s) 155, 740, 964, 1098, 3562, 3603
BcuI ACTAGT 1 cut(s) 2923
BfaI CTAG 7 cut(s) 1643, 2924, 3126, 3135, 3641, 3675, 4136
BfmI CTRYAG 8 cut(s) 168, 207, 252, 1314, 1728, 1939, 2412, 2598
BfrI CTTAAG 1 cut(s) 1979
BfuAI ACCTGC 3 cut(s) 2269, 2395, 3070
BfuI GTATCC 1 cut(s) 2963
BglII AGATCT 1 cut(s) 3575
BisI GCNGC 9 cut(s) 255, 592, 678, 884, 1186, 2601, 3513, 3613, 3810
BlsI GCNGC 9 cut(s) 256, 593, 679, 885, 1187, 2602, 3514, 3614, 3811
BmcAI AGTACT 4 cut(s) 571, 1807, 2858, 3031
Bme18I GGWCC 5 cut(s) 840, 1850, 2258, 3644, 3657
BmeT110I CYCGRG 2 cut(s) 1012, 3412
BmrI ACTGGG 2 cut(s) 958, 2503
BmsI GCATC 4 cut(s) 1259, 1774, 2093, 3851
BmuI ACTGGG 2 cut(s) 958, 2503
BplI GAGNNNNNCTC 4 cut(s) 1653, 1685, 2630, 2662
BpmI CTGGAG 2 cut(s) 2040, 3483
Bpu10I CCTNAGC 1 cut(s) 3838
BpuEI CTTGAG 3 cut(s) 798, 2957, 3592
BpuMI CCSGG 5 cut(s) 1013, 1014, 3413, 3414, 4160
BsaAI YACGTR 1 cut(s) 4199
BsaBI GATNNNNATC 2 cut(s) 9, 493
BsaI GGTCTC 2 cut(s) 964, 3603
BsaXI ACNNNNNCTCC 6 cut(s) 428, 458, 1725, 1755, 1854, 1884
Bse118I RCCGGY 1 cut(s) 1432
Bse1I ACTGG 9 cut(s) 147, 937, 953, 2498, 2550, 2690, 2893, 3206, 3772
Bse21I CCTNAGG 2 cut(s) 1697, 3328
Bse3DI GCAATG 2 cut(s) 601, 3867
Bse8I GATNNNNATC 2 cut(s) 9, 493
BseBI CCWGG 6 cut(s) 113, 744, 804, 1721, 1848, 2433
BseGI GGATG 5 cut(s) 532, 2775, 3068, 3074, 3692
BseJI GATNNNNATC 2 cut(s) 9, 493
BseMI GCAATG 2 cut(s) 601, 3867
BseNI ACTGG 9 cut(s) 147, 937, 953, 2498, 2550, 2690, 2893, 3206, 3772
BseRI GAGGAG 4 cut(s) 6, 2513, 2954, 3513
BseSI GKGCMC 5 cut(s) 87, 2239, 3125, 3703, 4204
BseXI GCAGC 9 cut(s) 266, 603, 689, 895, 1197, 2612, 3499, 3624, 3796
BseYI CCCAGC 4 cut(s) 362, 1188, 1601, 3757
BsgI GTGCAG 5 cut(s) 327, 2162, 2445, 3018, 3048
Bsh1285I CGRYCG 1 cut(s) 1161
BshFI GGCC 9 cut(s) 85, 192, 385, 1626, 3051, 3123, 3155, 3315, 4177
BshNI GGYRCC 2 cut(s) 978, 2024
BsiEI CGRYCG 1 cut(s) 1161
BsiHKAI GWGCWC 5 cut(s) 2239, 2899, 3091, 3703, 4204
BsiHKCI CYCGRG 2 cut(s) 1012, 3412
BsiSI CCGG 4 cut(s) 1013, 1433, 3413, 4160
BslFI GGGAC 8 cut(s) 982, 1420, 1697, 1836, 2271, 3349, 3670, 4141
BsmAI GTCTC 6 cut(s) 155, 740, 964, 1098, 3562, 3603
BsmFI GGGAC 8 cut(s) 982, 1420, 1697, 1836, 2271, 3349, 3670, 4141
BsmI GAATGC 1 cut(s) 814
BsnI GGCC 9 cut(s) 85, 192, 385, 1626, 3051, 3123, 3155, 3315, 4177
Bso31I GGTCTC 2 cut(s) 964, 3603
BsoBI CYCGRG 2 cut(s) 1012, 3412
Bsp120I GGGCCC 2 cut(s) 83, 3121
Bsp1286I GDGCHC 7 cut(s) 87, 2239, 2899, 3091, 3125, 3703, 4204
Bsp1407I TGTACA 3 cut(s) 1368, 2104, 3883
Bsp19I CCATGG 1 cut(s) 484
BspACI CCGC 7 cut(s) 41, 470, 1157, 2170, 2329, 2356, 3409
BspANI GGCC 9 cut(s) 85, 192, 385, 1626, 3051, 3123, 3155, 3315, 4177
BspHI TCATGA 1 cut(s) 4237
BspMAI CTGCAG 2 cut(s) 256, 2602
BspMI ACCTGC 3 cut(s) 2269, 2395, 3070
BspT107I GGYRCC 2 cut(s) 978, 2024
BspTI CTTAAG 1 cut(s) 1979
BspTNI GGTCTC 2 cut(s) 964, 3603
BsrDI GCAATG 2 cut(s) 601, 3867
BsrFI RCCGGY 1 cut(s) 1432
BsrGI TGTACA 3 cut(s) 1368, 2104, 3883
BsrI ACTGG 9 cut(s) 147, 937, 953, 2498, 2550, 2690, 2893, 3206, 3772
BssAI RCCGGY 1 cut(s) 1432
BssT1I CCWWGG 6 cut(s) 484, 1171, 1854, 3079, 3316, 3742
Bst2UI CCWGG 6 cut(s) 113, 744, 804, 1721, 1848, 2433
Bst6I CTCTTC 1 cut(s) 2830
BstAFI CTTAAG 1 cut(s) 1979
BstAPI GCANNNNNTGC 1 cut(s) 2149
BstAUI TGTACA 3 cut(s) 1368, 2104, 3883
BstBAI YACGTR 1 cut(s) 4199
BstC8I GCNNGC 9 cut(s) 1221, 1434, 1451, 1624, 2172, 2333, 2369, 2460, 2823
BstDSI CCRYGG 2 cut(s) 484, 3117
BstENI CCTNNNNNAGG 2 cut(s) 1731, 2157
BstF5I GGATG 5 cut(s) 532, 2775, 3068, 3074, 3692
BstHHI GCGC 2 cut(s) 2266, 2815
BstMAI GTCTC 6 cut(s) 155, 740, 964, 1098, 3562, 3603
BstMCI CGRYCG 1 cut(s) 1161
BstNI CCWGG 6 cut(s) 113, 744, 804, 1721, 1848, 2433
BstNSI RCATGY 3 cut(s) 316, 1640, 2821
BstSFI CTRYAG 8 cut(s) 168, 207, 252, 1314, 1728, 1939, 2412, 2598
BstSLI GKGCMC 5 cut(s) 87, 2239, 3125, 3703, 4204
BstV1I GCAGC 9 cut(s) 266, 603, 689, 895, 1197, 2612, 3499, 3624, 3796
BstX2I RGATCY 6 cut(s) 371, 480, 706, 1196, 1723, 3575
BstXI CCANNNNNNTGG 2 cut(s) 491, 3749
BstYI RGATCY 6 cut(s) 371, 480, 706, 1196, 1723, 3575
Bsu36I CCTNAGG 2 cut(s) 1697, 3328
BsuI GTATCC 1 cut(s) 2963
BsuRI GGCC 9 cut(s) 85, 192, 385, 1626, 3051, 3123, 3155, 3315, 4177
BtgI CCRYGG 2 cut(s) 484, 3117
BtsCI GGATG 5 cut(s) 532, 2775, 3068, 3074, 3692
BtsI GCAGTG 2 cut(s) 779, 3950
BveI ACCTGC 3 cut(s) 2269, 2395, 3070
Cac8I GCNNGC 9 cut(s) 1221, 1434, 1451, 1624, 2172, 2333, 2369, 2460, 2823
CaiI CAGNNNCTG 4 cut(s) 911, 1188, 1745, 2438
CciI TCATGA 1 cut(s) 4237
CfoI GCGC 2 cut(s) 2266, 2815
Cfr10I RCCGGY 1 cut(s) 1432
Cfr9I CCCGGG 2 cut(s) 1012, 3412
CspCI CAANNNNNGTGG 2 cut(s) 3727, 3762
DraI TTTAAA 1 cut(s) 1344
DraIII CACNNNGTG 3 cut(s) 2140, 2243, 3041
EaeI YGGCCR 3 cut(s) 1624, 3153, 3313
Eam1104I CTCTTC 1 cut(s) 2830
EarI CTCTTC 1 cut(s) 2830
EciI GGCGGA 2 cut(s) 56, 2318
Eco130I CCWWGG 6 cut(s) 484, 1171, 1854, 3079, 3316, 3742
Eco24I GRGCYC 2 cut(s) 87, 3125
Eco31I GGTCTC 2 cut(s) 964, 3603
Eco32I GATATC 1 cut(s) 541
Eco47I GGWCC 5 cut(s) 840, 1850, 2258, 3644, 3657
Eco57I CTGAAG 7 cut(s) 408, 2139, 2412, 2847, 2922, 3525, 3636
Eco72I CACGTG 1 cut(s) 4199
Eco81I CCTNAGG 2 cut(s) 1697, 3328
Eco88I CYCGRG 2 cut(s) 1012, 3412
EcoNI CCTNNNNNAGG 2 cut(s) 1731, 2157
EcoO109I RGGNCCY 6 cut(s) 83, 84, 1850, 2258, 3122, 3657
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 6 cut(s) 111, 742, 802, 1719, 1846, 2431
EcoRV GATATC 1 cut(s) 541
EcoT14I CCWWGG 6 cut(s) 484, 1171, 1854, 3079, 3316, 3742
EcoT38I GRGCYC 2 cut(s) 87, 3125
ErhI CCWWGG 6 cut(s) 484, 1171, 1854, 3079, 3316, 3742
FalI AAGNNNNNCTT 3 cut(s) 4054, 4086, 4233
FaqI GGGAC 8 cut(s) 982, 1420, 1697, 1836, 2271, 3349, 3670, 4141
FauI CCCGC 2 cut(s) 2163, 3416
FauNDI CATATG 2 cut(s) 1711, 4110
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 9 cut(s) 255, 592, 678, 884, 1186, 2601, 3513, 3613, 3810
FokI GGATG 5 cut(s) 539, 2762, 3055, 3061, 3679
FriOI GRGCYC 2 cut(s) 87, 3125
Fsp4HI GCNGC 9 cut(s) 255, 592, 678, 884, 1186, 2601, 3513, 3613, 3810
FspAI RTGCGCAY 1 cut(s) 2814
FspBI CTAG 7 cut(s) 1643, 2924, 3126, 3135, 3641, 3675, 4136
FspI TGCGCA 1 cut(s) 2814
GlaI GCGC 2 cut(s) 2265, 2814
GluI GCNGC 9 cut(s) 255, 592, 678, 884, 1186, 2601, 3513, 3613, 3810
GsaI CCCAGC 4 cut(s) 366, 1192, 1605, 3761
GsuI CTGGAG 2 cut(s) 2040, 3483
HaeIII GGCC 9 cut(s) 85, 192, 385, 1626, 3051, 3123, 3155, 3315, 4177
HapII CCGG 4 cut(s) 1013, 1433, 3413, 4160
HhaI GCGC 2 cut(s) 2266, 2815
Hin6I GCGC 2 cut(s) 2264, 2813
HinP1I GCGC 2 cut(s) 2264, 2813
HincII GTYRAC 3 cut(s) 1321, 1994, 3730
HindII GTYRAC 3 cut(s) 1321, 1994, 3730
HindIII AAGCTT 1 cut(s) 666
HpaI GTTAAC 1 cut(s) 1321
HpaII CCGG 4 cut(s) 1013, 1433, 3413, 4160
HphI GGTGA 8 cut(s) 257, 1406, 2340, 2685, 3297, 3446, 3768, 4238
Hpy99I CGWCG 3 cut(s) 3166, 3461, 3967
HpyCH4IV ACGT 4 cut(s) 339, 2912, 3726, 4198
HpySE526I ACGT 4 cut(s) 339, 2912, 3726, 4198
HspAI GCGC 2 cut(s) 2264, 2813
KflI GGGWCCC 1 cut(s) 3657
KpnI GGTACC 2 cut(s) 982, 2028
KroI GCCGGC 1 cut(s) 1432
KroNI GCCGGC 1 cut(s) 1434
KspAI GTTAAC 1 cut(s) 1321
LmnI GCTCC 7 cut(s) 104, 405, 419, 1603, 2675, 3952, 4091
Lsp1109I GCAGC 9 cut(s) 266, 603, 689, 895, 1197, 2612, 3499, 3624, 3796
LweI GCATC 4 cut(s) 1259, 1774, 2093, 3851
MaeI CTAG 7 cut(s) 1643, 2924, 3126, 3135, 3641, 3675, 4136
MaeII ACGT 4 cut(s) 339, 2912, 3726, 4198
MaeIII GTNAC 7 cut(s) 913, 1149, 1645, 2691, 3366, 3774, 4226
MboII GAAGA 7 cut(s) 1623, 2420, 2847, 3518, 3598, 3902, 4074
MfeI CAATTG 1 cut(s) 377
MflI RGATCY 6 cut(s) 371, 480, 706, 1196, 1723, 3575
MhlI GDGCHC 7 cut(s) 87, 2239, 2899, 3091, 3125, 3703, 4204
MlsI TGGCCA 2 cut(s) 3155, 3315
MluNI TGGCCA 2 cut(s) 3155, 3315
MlyI GAGTC 5 cut(s) 359, 2648, 2719, 2840, 4034
MmeI TCCRAC 4 cut(s) 786, 1840, 1852, 2812
Mox20I TGGCCA 2 cut(s) 3155, 3315
MroNI GCCGGC 1 cut(s) 1432
MroXI GAANNNNTTC 5 cut(s) 121, 2721, 2839, 3244, 4069
MscI TGGCCA 2 cut(s) 3155, 3315
MslI CAYNNNNRTG 2 cut(s) 1676, 3096
Msp20I TGGCCA 2 cut(s) 3155, 3315
MspA1I CMGCKG 7 cut(s) 257, 680, 1188, 2335, 2417, 3373, 3503
MspCI CTTAAG 1 cut(s) 1979
MspI CCGG 4 cut(s) 1013, 1433, 3413, 4160
MunI CAATTG 1 cut(s) 377
Mva1269I GAATGC 1 cut(s) 814
MvaI CCWGG 6 cut(s) 113, 744, 804, 1721, 1848, 2433
NaeI GCCGGC 1 cut(s) 1434
NciI CCSGG 5 cut(s) 1013, 1014, 3413, 3414, 4160
NcoI CCATGG 1 cut(s) 484
NdeI CATATG 2 cut(s) 1711, 4110
NgoMIV GCCGGC 1 cut(s) 1432
NmeAIII GCCGAG 1 cut(s) 4203
NmuCI GTSAC 6 cut(s) 913, 1149, 2691, 3366, 3774, 4226
NsbI TGCGCA 1 cut(s) 2814
NspI RCATGY 3 cut(s) 316, 1640, 2821
PagI TCATGA 1 cut(s) 4237
PaqCI CACCTGC 1 cut(s) 3070
PasI CCCWGGG 1 cut(s) 112
PciI ACATGT 1 cut(s) 312
PcsI WCGNNNNNNNCGW 1 cut(s) 3349
PctI GAATGC 1 cut(s) 814
PdiI GCCGGC 1 cut(s) 1434
PdmI GAANNNNTTC 5 cut(s) 121, 2721, 2839, 3244, 4069
PflMI CCANNNNNTGG 2 cut(s) 3260, 4211
PkrI GCNGC 9 cut(s) 256, 593, 679, 885, 1187, 2602, 3514, 3614, 3811
PleI GAGTC 5 cut(s) 358, 2647, 2718, 2840, 4034
PmaCI CACGTG 1 cut(s) 4199
PmlI CACGTG 1 cut(s) 4199
PpsI GAGTC 5 cut(s) 358, 2647, 2718, 2840, 4034
Ppu21I YACGTR 1 cut(s) 4199
PpuMI RGGWCCY 3 cut(s) 1850, 2258, 3657
PscI ACATGT 1 cut(s) 312
Psp5II RGGWCCY 3 cut(s) 1850, 2258, 3657
Psp6I CCWGG 6 cut(s) 111, 742, 802, 1719, 1846, 2431
PspCI CACGTG 1 cut(s) 4199
PspFI CCCAGC 4 cut(s) 362, 1188, 1601, 3757
PspGI CCWGG 6 cut(s) 111, 742, 802, 1719, 1846, 2431
PspOMI GGGCCC 2 cut(s) 83, 3121
PspPPI RGGWCCY 3 cut(s) 1850, 2258, 3657
PsrI GAACNNNNNNTAC 2 cut(s) 148, 180
PstI CTGCAG 2 cut(s) 256, 2602
PstNI CAGNNNCTG 4 cut(s) 911, 1188, 1745, 2438
PsuI RGATCY 6 cut(s) 371, 480, 706, 1196, 1723, 3575
PvuII CAGCTG 7 cut(s) 257, 680, 1188, 2335, 2417, 3373, 3503
RseI CAYNNNNRTG 2 cut(s) 1676, 3096
SatI GCNGC 9 cut(s) 255, 592, 678, 884, 1186, 2601, 3513, 3613, 3810
ScaI AGTACT 4 cut(s) 571, 1807, 2858, 3031
SchI GAGTC 5 cut(s) 359, 2648, 2719, 2840, 4034
SduI GDGCHC 7 cut(s) 87, 2239, 2899, 3091, 3125, 3703, 4204
SfaNI GCATC 4 cut(s) 1259, 1774, 2093, 3851
SfcI CTRYAG 8 cut(s) 168, 207, 252, 1314, 1728, 1939, 2412, 2598
SinI GGWCC 5 cut(s) 840, 1850, 2258, 3644, 3657
SmaI CCCGGG 2 cut(s) 1014, 3414
SmiMI CAYNNNNRTG 2 cut(s) 1676, 3096
SmlI CTYRAG 4 cut(s) 813, 1979, 2936, 3571
SmoI CTYRAG 4 cut(s) 813, 1979, 2936, 3571
SpeI ACTAGT 1 cut(s) 2923
SsiI CCGC 7 cut(s) 41, 470, 1157, 2170, 2329, 2356, 3409
SspI AATATT 2 cut(s) 2747, 4006
SspMI CTAG 7 cut(s) 1643, 2924, 3126, 3135, 3641, 3675, 4136
StyI CCWWGG 6 cut(s) 484, 1171, 1854, 3079, 3316, 3742
TaiI ACGT 4 cut(s) 342, 2915, 3729, 4201
TaqI TCGA 6 cut(s) 290, 317, 392, 1790, 2011, 3715
TseFI GTSAC 6 cut(s) 913, 1149, 2691, 3366, 3774, 4226
TseI GCWGC 9 cut(s) 254, 591, 677, 883, 1185, 2600, 3512, 3612, 3809
Tsp45I GTSAC 6 cut(s) 913, 1149, 2691, 3366, 3774, 4226
TspGWI ACGGA 2 cut(s) 1340, 1695
TspMI CCCGGG 2 cut(s) 1012, 3412
Van91I CCANNNNNTGG 2 cut(s) 3260, 4211
Vha464I CTTAAG 1 cut(s) 1979
VneI GTGCAC 3 cut(s) 2235, 3699, 4200
VpaK11BI GGWCC 5 cut(s) 840, 1850, 2258, 3644, 3657
XagI CCTNNNNNAGG 2 cut(s) 1731, 2157
XapI RAATTY 6 cut(s) 104, 1345, 2194, 2503, 2632, 2980
XceI RCATGY 3 cut(s) 316, 1640, 2821
XmaI CCCGGG 2 cut(s) 1012, 3412
XmiI GTMKAC 1 cut(s) 555
XmnI GAANNNNTTC 5 cut(s) 121, 2721, 2839, 3244, 4069
XspI CTAG 7 cut(s) 1643, 2924, 3126, 3135, 3641, 3675, 4136
ZrmI AGTACT 4 cut(s) 571, 1807, 2858, 3031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.