Protein transport protein sec16
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1200 |
| AarI | CACCTGC | 1 cut(s) | 3034 |
| Acc16I | TGCGCA | 1 cut(s) | 597 |
| Acc36I | ACCTGC | 4 cut(s) | 2198, 2359, 3034, 3466 |
| Acc65I | GGTACC | 2 cut(s) | 969, 1988 |
| AccB1I | GGYRCC | 4 cut(s) | 209, 353, 969, 1988 |
| AccB7I | CCANNNNNTGG | 7 cut(s) | 1274, 1291, 1571, 3212, 3811, 3961, 4154 |
| AccII | CGCG | 1 cut(s) | 104 |
| AcoI | YGGCCR | 2 cut(s) | 99, 1077 |
| AcsI | RAATTY | 2 cut(s) | 2467, 2596 |
| AcuI | CTGAAG | 7 cut(s) | 930, 2103, 2169, 2811, 2886, 3471, 3585 |
| AcyI | GRCGYC | 2 cut(s) | 150, 1591 |
| AdeI | CACNNNGTG | 3 cut(s) | 553, 1157, 2104 |
| AfaI | GTAC | 9 cut(s) | 739, 800, 971, 1013, 1990, 2154, 2822, 3577, 3834 |
| AfeI | AGCGCT | 1 cut(s) | 2229 |
| AflII | CTTAAG | 3 cut(s) | 1943, 3413, 3517 |
| AflIII | ACRYGT | 1 cut(s) | 3829 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1791, 1823, 3036, 3068 |
| Alw21I | GWGCWC | 5 cut(s) | 2071, 2203, 2724, 2863, 4034 |
| Alw26I | GTCTC | 5 cut(s) | 955, 1194, 2901, 3318, 3343 |
| Alw44I | GTGCAC | 2 cut(s) | 2067, 2199 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1709, 2618 |
| Aor51HI | AGCGCT | 1 cut(s) | 2229 |
| ApaI | GGGCCC | 1 cut(s) | 3566 |
| ApaLI | GTGCAC | 2 cut(s) | 2067, 2199 |
| ApeKI | GCWGC | 9 cut(s) | 578, 824, 874, 893, 1088, 2564, 2884, 3758, 4161 |
| ApoI | RAATTY | 2 cut(s) | 2467, 2596 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2588, 2620 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 139, 1579, 2803, 3196 |
| Asp718I | GGTACC | 2 cut(s) | 969, 1988 |
| AspLEI | GCGC | 3 cut(s) | 598, 2230, 4147 |
| AsuC2I | CCSGG | 3 cut(s) | 406, 974, 1588 |
| AsuII | TTCGAA | 1 cut(s) | 8 |
| AsuNHI | GCTAGC | 2 cut(s) | 914, 2672 |
| AvaII | GGWCC | 6 cut(s) | 116, 834, 3083, 3546, 3593, 3986 |
| AxyI | CCTNAGG | 2 cut(s) | 1239, 3274 |
| BaeGI | GKGCMC | 3 cut(s) | 2071, 2203, 3566 |
| BamHI | GGATCC | 3 cut(s) | 468, 482, 1687 |
| BanI | GGYRCC | 4 cut(s) | 209, 353, 969, 1988 |
| BanII | GRGCYC | 3 cut(s) | 1703, 3566, 4034 |
| BauI | CACGAG | 1 cut(s) | 3645 |
| BbsI | GAAGAC | 2 cut(s) | 576, 3481 |
| Bbv12I | GWGCWC | 5 cut(s) | 2071, 2203, 2724, 2863, 4034 |
| BbvI | GCAGC | 9 cut(s) | 590, 811, 880, 886, 1100, 2576, 2896, 3745, 4173 |
| BceAI | ACGGC | 5 cut(s) | 70, 1092, 1101, 1801, 4029 |
| BcgI | CGANNNNNNTGC | 8 cut(s) | 806, 840, 1007, 1041, 2310, 2344, 2975, 3009 |
| BciVI | GTATCC | 4 cut(s) | 28, 568, 771, 2927 |
| BclI | TGATCA | 2 cut(s) | 303, 997 |
| BcnI | CCSGG | 3 cut(s) | 406, 974, 1588 |
| BcoDI | GTCTC | 5 cut(s) | 955, 1194, 2901, 3318, 3343 |
| BfmI | CTRYAG | 6 cut(s) | 192, 1245, 1692, 1903, 2562, 3483 |
| BfoI | RGCGCY | 1 cut(s) | 2231 |
| BfrI | CTTAAG | 3 cut(s) | 1943, 3413, 3517 |
| BfuAI | ACCTGC | 4 cut(s) | 2198, 2359, 3034, 3466 |
| BfuI | GTATCC | 4 cut(s) | 28, 568, 771, 2927 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 257, 455, 1085 |
| BglII | AGATCT | 1 cut(s) | 1577 |
| BlpI | GCTNAGC | 1 cut(s) | 2037 |
| BmcAI | AGTACT | 1 cut(s) | 2822 |
| Bme18I | GGWCC | 6 cut(s) | 116, 834, 3083, 3546, 3593, 3986 |
| BmrI | ACTGGG | 1 cut(s) | 531 |
| BmsI | GCATC | 7 cut(s) | 238, 253, 1738, 2057, 3171, 3832, 4025 |
| BmtI | GCTAGC | 2 cut(s) | 918, 2676 |
| BmuI | ACTGGG | 1 cut(s) | 531 |
| BpiI | GAAGAC | 2 cut(s) | 576, 3481 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2594, 2626 |
| BpmI | CTGGAG | 4 cut(s) | 3429, 3573, 3717, 3771 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1092 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2037 |
| Bpu14I | TTCGAA | 1 cut(s) | 8 |
| BpuMI | CCSGG | 3 cut(s) | 406, 974, 1588 |
| Bsa29I | ATCGAT | 1 cut(s) | 2985 |
| BsaAI | YACGTR | 1 cut(s) | 3575 |
| BsaHI | GRCGYC | 2 cut(s) | 150, 1591 |
| BsaI | GGTCTC | 4 cut(s) | 955, 1194, 3318, 3343 |
| BsaWI | WCCGGW | 1 cut(s) | 206 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 851, 881, 1546, 1576, 1818, 1848, 3568, 3598 |
| Bse1I | ACTGG | 7 cut(s) | 526, 928, 2169, 2296, 2514, 2654, 3721 |
| Bse21I | CCTNAGG | 2 cut(s) | 1239, 3274 |
| Bse3DI | GCAATG | 6 cut(s) | 346, 643, 800, 2068, 3123, 3461 |
| BseCI | ATCGAT | 1 cut(s) | 2985 |
| BseGI | GGATG | 9 cut(s) | 46, 253, 268, 520, 733, 2112, 3032, 3038, 3122 |
| BseMI | GCAATG | 6 cut(s) | 346, 643, 800, 2068, 3123, 3461 |
| BseNI | ACTGG | 7 cut(s) | 526, 928, 2169, 2296, 2514, 2654, 3721 |
| BseRI | GAGGAG | 6 cut(s) | 6, 239, 2477, 2918, 3459, 3576 |
| BseSI | GKGCMC | 3 cut(s) | 2071, 2203, 3566 |
| BseX3I | CGGCCG | 1 cut(s) | 99 |
| BseXI | GCAGC | 9 cut(s) | 590, 811, 880, 886, 1100, 2576, 2896, 3745, 4173 |
| BseYI | CCCAGC | 2 cut(s) | 939, 3623 |
| BsgI | GTGCAG | 4 cut(s) | 642, 2126, 2447, 3012 |
| Bsh1236I | CGCG | 1 cut(s) | 104 |
| Bsh1285I | CGRYCG | 1 cut(s) | 102 |
| BshNI | GGYRCC | 4 cut(s) | 209, 353, 969, 1988 |
| BshVI | ATCGAT | 1 cut(s) | 2985 |
| BsiEI | CGRYCG | 1 cut(s) | 102 |
| BsiHKAI | GWGCWC | 5 cut(s) | 2071, 2203, 2724, 2863, 4034 |
| BslFI | GGGAC | 9 cut(s) | 129, 764, 973, 1405, 3295, 3532, 3619, 3972, 4084 |
| BsmAI | GTCTC | 5 cut(s) | 955, 1194, 2901, 3318, 3343 |
| BsmFI | GGGAC | 9 cut(s) | 129, 764, 973, 1405, 3295, 3532, 3619, 3972, 4084 |
| BsmI | GAATGC | 1 cut(s) | 3708 |
| Bso31I | GGTCTC | 4 cut(s) | 955, 1194, 3318, 3343 |
| Bsp119I | TTCGAA | 1 cut(s) | 8 |
| Bsp120I | GGGCCC | 1 cut(s) | 3562 |
| Bsp1286I | GDGCHC | 7 cut(s) | 1703, 2071, 2203, 2724, 2863, 3566, 4034 |
| Bsp1407I | TGTACA | 1 cut(s) | 3832 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2037 |
| Bsp19I | CCATGG | 1 cut(s) | 472 |
| BspDI | ATCGAT | 1 cut(s) | 2985 |
| BspFNI | CGCG | 1 cut(s) | 104 |
| BspHI | TCATGA | 2 cut(s) | 1120, 4183 |
| BspMAI | CTGCAG | 2 cut(s) | 2566, 3487 |
| BspMI | ACCTGC | 4 cut(s) | 2198, 2359, 3034, 3466 |
| BspOI | GCTAGC | 2 cut(s) | 918, 2676 |
| BspT104I | TTCGAA | 1 cut(s) | 8 |
| BspT107I | GGYRCC | 4 cut(s) | 209, 353, 969, 1988 |
| BspTI | CTTAAG | 3 cut(s) | 1943, 3413, 3517 |
| BspTNI | GGTCTC | 4 cut(s) | 955, 1194, 3318, 3343 |
| BsrDI | GCAATG | 6 cut(s) | 346, 643, 800, 2068, 3123, 3461 |
| BsrGI | TGTACA | 1 cut(s) | 3832 |
| BsrI | ACTGG | 7 cut(s) | 526, 928, 2169, 2296, 2514, 2654, 3721 |
| BssNI | GRCGYC | 2 cut(s) | 150, 1591 |
| BssSI | CACGAG | 1 cut(s) | 3645 |
| BssT1I | CCWWGG | 5 cut(s) | 153, 472, 1168, 2004, 3043 |
| Bst2BI | CACGAG | 1 cut(s) | 3645 |
| Bst6I | CTCTTC | 3 cut(s) | 1675, 2794, 3534 |
| BstACI | GRCGYC | 2 cut(s) | 150, 1591 |
| BstAFI | CTTAAG | 3 cut(s) | 1943, 3413, 3517 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1058 |
| BstAUI | TGTACA | 1 cut(s) | 3832 |
| BstBAI | YACGTR | 1 cut(s) | 3575 |
| BstBI | TTCGAA | 1 cut(s) | 8 |
| BstDSI | CCRYGG | 3 cut(s) | 102, 472, 1074 |
| BstEII | GGTNACC | 1 cut(s) | 3800 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2121 |
| BstF5I | GGATG | 9 cut(s) | 46, 253, 268, 520, 733, 2112, 3032, 3038, 3122 |
| BstFNI | CGCG | 1 cut(s) | 104 |
| BstH2I | RGCGCY | 1 cut(s) | 2231 |
| BstHHI | GCGC | 3 cut(s) | 598, 2230, 4147 |
| BstMAI | GTCTC | 5 cut(s) | 955, 1194, 2901, 3318, 3343 |
| BstMCI | CGRYCG | 1 cut(s) | 102 |
| BstNSI | RCATGY | 5 cut(s) | 1604, 2785, 3833, 4099, 4145 |
| BstPI | GGTNACC | 1 cut(s) | 3800 |
| BstSFI | CTRYAG | 6 cut(s) | 192, 1245, 1692, 1903, 2562, 3483 |
| BstSLI | GKGCMC | 3 cut(s) | 2071, 2203, 3566 |
| BstSNI | TACGTA | 1 cut(s) | 3575 |
| BstUI | CGCG | 1 cut(s) | 104 |
| BstV1I | GCAGC | 9 cut(s) | 590, 811, 880, 886, 1100, 2576, 2896, 3745, 4173 |
| BstV2I | GAAGAC | 2 cut(s) | 576, 3481 |
| BstX2I | RGATCY | 6 cut(s) | 468, 482, 1577, 1687, 3665, 3862 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 479 |
| BstYI | RGATCY | 6 cut(s) | 468, 482, 1577, 1687, 3665, 3862 |
| BstZI | CGGCCG | 1 cut(s) | 99 |
| Bsu15I | ATCGAT | 1 cut(s) | 2985 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1239, 3274 |
| BsuI | GTATCC | 4 cut(s) | 28, 568, 771, 2927 |
| BsuTUI | ATCGAT | 1 cut(s) | 2985 |
| BtgI | CCRYGG | 3 cut(s) | 102, 472, 1074 |
| BtsCI | GGATG | 9 cut(s) | 46, 253, 268, 520, 733, 2112, 3032, 3038, 3122 |
| BtsI | GCAGTG | 1 cut(s) | 571 |
| BtsIMutI | CAGTG | 5 cut(s) | 519, 571, 2098, 2395, 2647 |
| BveI | ACCTGC | 4 cut(s) | 2198, 2359, 3034, 3466 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1709, 2618 |
| CciI | TCATGA | 2 cut(s) | 1120, 4183 |
| CciNI | GCGGCCGC | 1 cut(s) | 99 |
| CfoI | GCGC | 3 cut(s) | 598, 2230, 4147 |
| Cfr42I | CCGCGG | 1 cut(s) | 105 |
| ClaI | ATCGAT | 1 cut(s) | 2985 |
| CseI | GACGC | 2 cut(s) | 158, 1580 |
| Csp6I | GTAC | 9 cut(s) | 738, 799, 970, 1012, 1989, 2153, 2821, 3576, 3833 |
| CviQI | GTAC | 9 cut(s) | 738, 799, 970, 1012, 1989, 2153, 2821, 3576, 3833 |
| DraI | TTTAAA | 1 cut(s) | 3531 |
| DraIII | CACNNNGTG | 3 cut(s) | 553, 1157, 2104 |
| EaeI | YGGCCR | 2 cut(s) | 99, 1077 |
| EagI | CGGCCG | 1 cut(s) | 99 |
| Eam1104I | CTCTTC | 3 cut(s) | 1675, 2794, 3534 |
| EarI | CTCTTC | 3 cut(s) | 1675, 2794, 3534 |
| EciI | GGCGGA | 3 cut(s) | 215, 275, 332 |
| Ecl136II | GAGCTC | 1 cut(s) | 4032 |
| EclXI | CGGCCG | 1 cut(s) | 99 |
| Eco105I | TACGTA | 1 cut(s) | 3575 |
| Eco130I | CCWWGG | 5 cut(s) | 153, 472, 1168, 2004, 3043 |
| Eco147I | AGGCCT | 1 cut(s) | 4117 |
| Eco24I | GRGCYC | 3 cut(s) | 1703, 3566, 4034 |
| Eco31I | GGTCTC | 4 cut(s) | 955, 1194, 3318, 3343 |
| Eco32I | GATATC | 1 cut(s) | 612 |
| Eco47I | GGWCC | 6 cut(s) | 116, 834, 3083, 3546, 3593, 3986 |
| Eco47III | AGCGCT | 1 cut(s) | 2229 |
| Eco52I | CGGCCG | 1 cut(s) | 99 |
| Eco53kI | GAGCTC | 1 cut(s) | 4032 |
| Eco57I | CTGAAG | 7 cut(s) | 930, 2103, 2169, 2811, 2886, 3471, 3585 |
| Eco81I | CCTNAGG | 2 cut(s) | 1239, 3274 |
| Eco91I | GGTNACC | 1 cut(s) | 3800 |
| EcoICRI | GAGCTC | 1 cut(s) | 4032 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2121 |
| EcoO109I | RGGNCCY | 4 cut(s) | 116, 3083, 3546, 3562 |
| EcoO65I | GGTNACC | 1 cut(s) | 3800 |
| EcoRV | GATATC | 1 cut(s) | 612 |
| EcoT14I | CCWWGG | 5 cut(s) | 153, 472, 1168, 2004, 3043 |
| EcoT22I | ATGCAT | 2 cut(s) | 3186, 4143 |
| EcoT38I | GRGCYC | 3 cut(s) | 1703, 3566, 4034 |
| ErhI | CCWWGG | 5 cut(s) | 153, 472, 1168, 2004, 3043 |
| FalI | AAGNNNNNCTT | 5 cut(s) | 1861, 1893, 3511, 3543, 4179 |
| FaqI | GGGAC | 9 cut(s) | 129, 764, 973, 1405, 3295, 3532, 3619, 3972, 4084 |
| FauI | CCCGC | 5 cut(s) | 106, 343, 541, 1690, 3296 |
| FauNDI | CATATG | 3 cut(s) | 981, 1558, 2668 |
| FbaI | TGATCA | 2 cut(s) | 303, 997 |
| FokI | GGATG | 9 cut(s) | 53, 260, 275, 527, 740, 2099, 3019, 3025, 3109 |
| FriOI | GRGCYC | 3 cut(s) | 1703, 3566, 4034 |
| FspI | TGCGCA | 1 cut(s) | 597 |
| GlaI | GCGC | 3 cut(s) | 597, 2229, 4146 |
| GsaI | CCCAGC | 2 cut(s) | 943, 3627 |
| GsuI | CTGGAG | 4 cut(s) | 3429, 3573, 3717, 3771 |
| HaeII | RGCGCY | 1 cut(s) | 2231 |
| HgaI | GACGC | 2 cut(s) | 158, 1580 |
| HhaI | GCGC | 3 cut(s) | 598, 2230, 4147 |
| Hin1I | GRCGYC | 2 cut(s) | 150, 1591 |
| Hin6I | GCGC | 3 cut(s) | 596, 2228, 4145 |
| HinP1I | GCGC | 3 cut(s) | 596, 2228, 4145 |
| HincII | GTYRAC | 3 cut(s) | 1318, 1958, 3679 |
| HindII | GTYRAC | 3 cut(s) | 1318, 1958, 3679 |
| HindIII | AAGCTT | 2 cut(s) | 687, 2039 |
| HpaI | GTTAAC | 1 cut(s) | 1318 |
| Hpy99I | CGWCG | 5 cut(s) | 91, 94, 97, 149, 152 |
| HpyCH4IV | ACGT | 2 cut(s) | 2876, 3574 |
| HpySE526I | ACGT | 2 cut(s) | 2876, 3574 |
| Hsp92I | GRCGYC | 2 cut(s) | 150, 1591 |
| HspAI | GCGC | 3 cut(s) | 596, 2228, 4145 |
| KflI | GGGWCCC | 2 cut(s) | 116, 3546 |
| KpnI | GGTACC | 2 cut(s) | 973, 1992 |
| Ksp22I | TGATCA | 2 cut(s) | 303, 997 |
| KspAI | GTTAAC | 1 cut(s) | 1318 |
| KspI | CCGCGG | 1 cut(s) | 105 |
| LmnI | GCTCC | 9 cut(s) | 695, 821, 1340, 1567, 2639, 3012, 3904, 4029, 4037 |
| Lsp1109I | GCAGC | 9 cut(s) | 590, 811, 880, 886, 1100, 2576, 2896, 3745, 4173 |
| LweI | GCATC | 7 cut(s) | 238, 253, 1738, 2057, 3171, 3832, 4025 |
| MaeII | ACGT | 2 cut(s) | 2876, 3574 |
| MaeIII | GTNAC | 9 cut(s) | 841, 904, 1149, 1163, 1609, 1926, 3723, 3800, 4172 |
| MfeI | CAATTG | 2 cut(s) | 1023, 2213 |
| MflI | RGATCY | 6 cut(s) | 468, 482, 1577, 1687, 3665, 3862 |
| MhlI | GDGCHC | 7 cut(s) | 1703, 2071, 2203, 2724, 2863, 3566, 4034 |
| MlyI | GAGTC | 5 cut(s) | 299, 341, 1268, 2612, 3881 |
| MmeI | TCCRAC | 6 cut(s) | 168, 1552, 1589, 1804, 1816, 2776 |
| Mph1103I | ATGCAT | 2 cut(s) | 3186, 4143 |
| MroXI | GAANNNNTTC | 4 cut(s) | 139, 1579, 2803, 3196 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1125, 1640 |
| MspA1I | CMGCKG | 3 cut(s) | 104, 3319, 3449 |
| MspCI | CTTAAG | 3 cut(s) | 1943, 3413, 3517 |
| MunI | CAATTG | 2 cut(s) | 1023, 2213 |
| Mva1269I | GAATGC | 1 cut(s) | 3708 |
| MvnI | CGCG | 1 cut(s) | 104 |
| NciI | CCSGG | 3 cut(s) | 406, 974, 1588 |
| NcoI | CCATGG | 1 cut(s) | 472 |
| NdeI | CATATG | 3 cut(s) | 981, 1558, 2668 |
| NheI | GCTAGC | 2 cut(s) | 914, 2672 |
| NmuCI | GTSAC | 5 cut(s) | 904, 1149, 3723, 3800, 4172 |
| NotI | GCGGCCGC | 1 cut(s) | 99 |
| NsbI | TGCGCA | 1 cut(s) | 597 |
| NsiI | ATGCAT | 2 cut(s) | 3186, 4143 |
| NspI | RCATGY | 5 cut(s) | 1604, 2785, 3833, 4099, 4145 |
| NspV | TTCGAA | 1 cut(s) | 8 |
| PaeI | GCATGC | 1 cut(s) | 4145 |
| PagI | TCATGA | 2 cut(s) | 1120, 4183 |
| PaqCI | CACCTGC | 1 cut(s) | 3034 |
| PceI | AGGCCT | 1 cut(s) | 4117 |
| PciI | ACATGT | 1 cut(s) | 3829 |
| PctI | GAATGC | 1 cut(s) | 3708 |
| PdmI | GAANNNNTTC | 4 cut(s) | 139, 1579, 2803, 3196 |
| PflFI | GACNNNGTC | 1 cut(s) | 2628 |
| PflMI | CCANNNNNTGG | 7 cut(s) | 1274, 1291, 1571, 3212, 3811, 3961, 4154 |
| PfoI | TCCNGGA | 2 cut(s) | 2305, 3589 |
| PleI | GAGTC | 5 cut(s) | 298, 340, 1267, 2611, 3881 |
| PpsI | GAGTC | 5 cut(s) | 298, 340, 1267, 2611, 3881 |
| Ppu21I | YACGTR | 1 cut(s) | 3575 |
| PpuMI | RGGWCCY | 3 cut(s) | 116, 3083, 3546 |
| PscI | ACATGT | 1 cut(s) | 3829 |
| PsiI | TTATAA | 1 cut(s) | 1200 |
| Psp124BI | GAGCTC | 1 cut(s) | 4034 |
| Psp5II | RGGWCCY | 3 cut(s) | 116, 3083, 3546 |
| PspEI | GGTNACC | 1 cut(s) | 3800 |
| PspFI | CCCAGC | 2 cut(s) | 939, 3623 |
| PspOMI | GGGCCC | 1 cut(s) | 3562 |
| PspPPI | RGGWCCY | 3 cut(s) | 116, 3083, 3546 |
| PstI | CTGCAG | 2 cut(s) | 2566, 3487 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1709, 2618 |
| PsuI | RGATCY | 6 cut(s) | 468, 482, 1577, 1687, 3665, 3862 |
| PsyI | GACNNNGTC | 1 cut(s) | 2628 |
| PvuII | CAGCTG | 2 cut(s) | 3319, 3449 |
| RsaI | GTAC | 9 cut(s) | 739, 800, 971, 1013, 1990, 2154, 2822, 3577, 3834 |
| RsaNI | GTAC | 9 cut(s) | 738, 799, 970, 1012, 1989, 2153, 2821, 3576, 3833 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1125, 1640 |
| SacI | GAGCTC | 1 cut(s) | 4034 |
| SacII | CCGCGG | 1 cut(s) | 105 |
| ScaI | AGTACT | 1 cut(s) | 2822 |
| SchI | GAGTC | 5 cut(s) | 299, 341, 1268, 2612, 3881 |
| SduI | GDGCHC | 7 cut(s) | 1703, 2071, 2203, 2724, 2863, 3566, 4034 |
| SfaNI | GCATC | 7 cut(s) | 238, 253, 1738, 2057, 3171, 3832, 4025 |
| SfcI | CTRYAG | 6 cut(s) | 192, 1245, 1692, 1903, 2562, 3483 |
| Sfr303I | CCGCGG | 1 cut(s) | 105 |
| SfuI | TTCGAA | 1 cut(s) | 8 |
| SgrBI | CCGCGG | 1 cut(s) | 105 |
| SinI | GGWCC | 6 cut(s) | 116, 834, 3083, 3546, 3593, 3986 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1125, 1640 |
| SmlI | CTYRAG | 3 cut(s) | 1943, 3413, 3517 |
| SmoI | CTYRAG | 3 cut(s) | 1943, 3413, 3517 |
| SnaBI | TACGTA | 1 cut(s) | 3575 |
| SphI | GCATGC | 1 cut(s) | 4145 |
| SseBI | AGGCCT | 1 cut(s) | 4117 |
| SspI | AATATT | 2 cut(s) | 2711, 2835 |
| SstI | GAGCTC | 1 cut(s) | 4034 |
| StuI | AGGCCT | 1 cut(s) | 4117 |
| StyI | CCWWGG | 5 cut(s) | 153, 472, 1168, 2004, 3043 |
| TaiI | ACGT | 2 cut(s) | 2879, 3577 |
| TaqI | TCGA | 7 cut(s) | 8, 66, 78, 293, 816, 2662, 2985 |
| TatI | WGTACW | 3 cut(s) | 2152, 2820, 3832 |
| TauI | GCSGC | 7 cut(s) | 101, 104, 107, 191, 200, 4129, 4161 |
| TscAI | CASTG | 5 cut(s) | 526, 571, 2105, 2395, 2654 |
| TseFI | GTSAC | 5 cut(s) | 904, 1149, 3723, 3800, 4172 |
| TseI | GCWGC | 9 cut(s) | 578, 824, 874, 893, 1088, 2564, 2884, 3758, 4161 |
| Tsp45I | GTSAC | 5 cut(s) | 904, 1149, 3723, 3800, 4172 |
| TspGWI | ACGGA | 1 cut(s) | 53 |
| TspRI | CASTG | 5 cut(s) | 526, 571, 2105, 2395, 2654 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2628 |
| Van91I | CCANNNNNTGG | 7 cut(s) | 1274, 1291, 1571, 3212, 3811, 3961, 4154 |
| Vha464I | CTTAAG | 3 cut(s) | 1943, 3413, 3517 |
| VneI | GTGCAC | 2 cut(s) | 2067, 2199 |
| VpaK11BI | GGWCC | 6 cut(s) | 116, 834, 3083, 3546, 3593, 3986 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2121 |
| XapI | RAATTY | 2 cut(s) | 2467, 2596 |
| XbaI | TCTAGA | 1 cut(s) | 2899 |
| XceI | RCATGY | 5 cut(s) | 1604, 2785, 3833, 4099, 4145 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 3005 |
| XmnI | GAANNNNTTC | 4 cut(s) | 139, 1579, 2803, 3196 |
| ZrmI | AGTACT | 1 cut(s) | 2822 |
| Zsp2I | ATGCAT | 2 cut(s) | 3186, 4143 |