Rroxscaffold_3G00253830

Protein transport protein sec16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001182
Physical Location & Seq
Forward (+)
47898040 .. 48053774
155735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00253830.1

Sequence Viewer

Length: 3489 bp
ATGGCTTCGAATCCTCCTCCATTTCAGGTGGAGGATACGGATGAGGATTTCTTTGATAAATTGGTCGAAGATGACATCGAACCGCTGTCGTCGTCGGCGGTGGCGGGACCCAATTTGAATGAGGGAAGTGATTCCGACGACGCCAAGGCGTTTGCTAATCTGAGCGTTGGTGATTCTGCGGCTGTAGCGGCGGATTCCGGTGCCGGAAAGAAAGAGGAGAAGGGCGGTGTGGATTTGGATGCCGGAGAGGCGGATGCGGTTGGGGTTGGTAATGTGGTCGAGTCGAGTAATGTGATCAATGGTTCGTCGGAGGGGGCGGTGGAGTCCAGCAATGATGGGGCGGGTGCTGAATTGAAGCCGGATTCAGATTCGGTGGTGAGCAAGAGCAATGATTCCGGGTTGGGGGATGGCACTGGGGATTTTCCCGCCAAAGTGGAACACAATGTGGTATCCGGCAGTGAAGATTATACAGCCAACAGTTTGACTGCGCAACCAAATGTGATATCCGGCAGTGAAGATTATACAGCCAATGGTTTGAGCAATTCGGTTAATAATAGCCAGCACTATCAAGAGGTTCAAGCTTATGGAGCCTCCGGGGAAGAAAGCAAAAACGAGTCGGATGTAAACAGTACTGAGTATTGGGACAGTCTGTATCCTGGGTGGAAGTATGACCCCAACACAGGGCAATGGTATGAGGTGGACAGTTTCGACGGAGCTGCTAATTGGACCACCGTTACTGATACTAAACAGGAGGTTTCGTATTTGCAGCAAACAGCTCACTCTGCTACCGGAACTGTGACTGAAGCTAGCACAACTGGTTGTTTGTCTAGCTGGGAACAGGTCTCACAGGGGACTAATGGGTACCCGGAACATATGGTTTTTTATCCTGATCACCCTGATTGGTACTACGACACAATTGCCCAGGAGTGGCGTTCATTGGAAGCATATAATGCTTCTATGCAACCCACGGCCACAACGGCAGCTCAGGCCCAGAATGAGAATAGTTTATATCATGAGTATGGACAAGCTGAAAATTATGGGTCACAAGGTGTCAGTAACCAAGGTCAAGAGGGTAGCTGGTCTCAGTCTTATAATAATTACAATCAGCAAGTTTCAAATATGTGGCAACCTGAGGCTGTAGCTACCAGCGAGTCTTTTCCAAGTTTTGGTGGAAACCAACAGTTGGATAATTCATTAGCTTCTATGGTTAACAATGATCAGCAGAAGCCGGTCAGCTCTTTCGAGCAGTTCCTTTGTATAACAAAGCAAGTCAGGGTCATGGTGAGGCTAGTGGGACTTTTGGGTTCCAAAGTTTCGCCCCAGAGTGGCAATCAGTTTACATATGCTCCAACTGTTGGAAGATCTTCTGCCGGGCGTCCTCCACATGCCCTAGTAACTTTTGGATTTGGTGGAAAACTCATTGTGATGAAGGACGGTAGTAGTCTTGGGAACTCTTCCTATGGAAGCCAGGATCCTGGAGCAGGCTCAGTTTCTGTTCTGAACTTGACAGAAGTTTTCATGCAAAAGCCTGATGCTTCAAGTTTTGGAATGACTACTTGTGATTATTTTCGTGCCTTGTGCCAACAGTCCTTTCCAGGGCCGTTGGTTGGAGGGAATGTTGGAAGTAAAGAGTTGAACAAATGGATTGATGAAAGGATTGCTTACTGTGAATCACCTGACATGGACTATAGGAAAGGAAAAGTATTGAGGTTACTGATTTCTTTGCTTAAGATAGCTTGTCAACACTATGGAAAACTCCGATCTCCTTTTGGTACCGACACTCTATCCAAGGAAAATGATATTCCAGAATCCGCAGTTGCTAAGCTTTTTGCATCTGCAAAGAGCAATGGTGCACAGCTCGGTGAGTATAATGCTCTTAGCCACTGTGTGCAGAACATCCCTTCAGAAGGACAAATACAGGCAACTGCTTCTGAAGTACAAAATCTACTGGTTTCTGGTAGAAAGAAAGAGGCTTTACAATGTGCACAGGCAGGCCAACCTGCGGAAGTGTTTTCTGCTGACACTACATCTGAAATCAGTTTTCCTGGTGCTGTTAATACGCCTCAACAGCCTGTGCAGGATAGGAGTGAGATTACTGCAGCACACATCTGCTATTTAATTGCAGAAGCAAATTTTGAGTCATACTCAGATACTGCAAGACTATGTCTAATTGGAGCAGATCACTGGAAATTGCCTCGAACGTATGCTAGTCCAGAGGCCATTCAGAGGACTGAGTTATATGAATATTCAAAGGTGCTCGGAAACTCTCAGTTTATTCTTCTACCATTCCAGCCATATAAGCTCATATATGCACACATGCTTGCTGAAGTTGGAAGAGTTTCTGATTCGTTGAAGTACTGTCAAATAATATTGAAGTCCTTAAAAACTGTTAGAGCACCTGAAGTGGAAACATGGAAGCAGCTAGTATTATCTCTAGAGGAGAGGATCAGAACTCATCAGCAGGGTGGATACTCTGTAAATCTGGTTTCTACTTTCAAAGGTAAATTACTCAACTTTATCGATACTACTGCCCATCGTGTTGTTGGGAGCCTACCACCACCTGCACCATCCACATCCCAAGGAAGTGTTCACGGTAATCATTACCAACAGCCCATGGGCCCTAGAGTATCTAGTAGTCAATCAACTATGGCAATGTCATCCTTAATTCCTTCTGCTTCTATGGAACCCATAAGTGATTTTGGCAATAGAAAGCCGATGCATAATAGAAGTGTTTCAGAGCCAGACTTTGGCAGAACTCAGCTGTTGCAAAAGACTGTGGGCCTAGTCCTGAGACCTCGCCCTGGAAAACAGGCTAAGTTGGGTGAAACAAATAAGTTCTACTATGACGAAAAACTTAAGAGATGGGTTGAGGAAGGTGCTGAACCTCCAGCTGAAGAACCTGCATTGCCTCCTCCCCCAACAACTGCAGTCTTCCAGAATGGTGTTTCTGATTACAACTTAAGATATGTTGACACTTTCAACCAAGCTGGTGGAATGCCAGCAAACTTGTTCCAGTCACCTGCTGTTCCATCTATTAAGCCTGCTGTTGCTGCCAATGCAAAGTTTTTCATTCCCACTCCAGCGTTATCAGGTGACCAGACTATGGAGGCTATTGCTGAACATGTACAAGAAGATGCTACAACAAAAGAAGATCCTTCAACATCAGCCAGGAATGACTCTTTTCATGCTCCACCTTCATCATCACCAATGAGTATGCAAAGGTACCCTAGCATGAATAATATCCAAAGCGTGGGAGTTATGTCAAATGCCAATGGTCCCCTTCCAGCTCACTCACGAAGGACGGCATCATGGGGTGGGAGCTCCAATGATATATTCAGCCCTCCAAAGATGGGAGAAATAAAACCTCTAGGGGAGGCATTAGGCATGTCCCCTACTTTATATAGGCCTAGTGAGCCGCCTTCTCAAATGCATGCGCCAGTGAATGGCGGCGGCAGTTTTGGTGACGATCTTCATGAAGTGGAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec16 Sec16_C

Protein Analysis

1162

Amino Acids

125.22

Weight (kDa)

4.8

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec16 PF12932 462 - 584 4.5e-21 Vesicle coat trafficking protein Sec16 mid-region
TPR_Sec16 PF12931 695 - 822 1.6e-24 Sec16 Sec23-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1092
AarI CACCTGC 2 cut(s) 2569, 3028
Acc16I TGCGCA 1 cut(s) 489
Acc36I ACCTGC 4 cut(s) 2008, 2569, 2908, 3028
Acc65I GGTACC 3 cut(s) 861, 1772, 3222
AccB1I GGYRCC 4 cut(s) 200, 861, 1772, 3222
AccB7I CCANNNNNTGG 7 cut(s) 1166, 1183, 1355, 2747, 3103, 3250, 3443
AclWI GGATC 4 cut(s) 1466, 1479, 2453, 3146
AcoI YGGCCR 1 cut(s) 969
AcsI RAATTY 1 cut(s) 2131
AcuI CTGAAG 6 cut(s) 822, 1887, 1953, 2346, 2421, 2913
AcyI GRCGYC 2 cut(s) 141, 1375
AdeI CACNNNGTG 3 cut(s) 445, 1049, 1888
AfaI GTAC 8 cut(s) 631, 863, 905, 1774, 1938, 2357, 3126, 3224
AflII CTTAAG 3 cut(s) 1727, 2855, 2959
AflIII ACRYGT 1 cut(s) 3121
AjnI CCWGG 8 cut(s) 655, 921, 1467, 1474, 1594, 2044, 2800, 3167
AjuI GAANNNNNNNTTGG 6 cut(s) 1575, 1607, 2571, 2603, 3317, 3349
Alw21I GWGCWC 5 cut(s) 1855, 1987, 2259, 2398, 3323
Alw26I GTCTC 3 cut(s) 847, 1086, 2785
Alw44I GTGCAC 2 cut(s) 1851, 1983
AlwI GGATC 4 cut(s) 1466, 1479, 2453, 3146
AlwNI CAGNNNCTG 2 cut(s) 1493, 2153
AoxI GGCC 8 cut(s) 969, 987, 1598, 1993, 2217, 2617, 2779, 3404
ApaI GGGCCC 1 cut(s) 2621
ApaLI GTGCAC 2 cut(s) 1851, 1983
ApeKI GCWGC 6 cut(s) 716, 766, 980, 2099, 2419, 3050
ApoI RAATTY 1 cut(s) 2131
ArsI GACNNNNNNTTYG 2 cut(s) 2123, 2155
Asp700I GAANNNNTTC 4 cut(s) 130, 1363, 2338, 2731
Asp718I GGTACC 3 cut(s) 861, 1772, 3222
AspLEI GCGC 2 cut(s) 490, 3436
AspS9I GGNCC 8 cut(s) 107, 726, 988, 1598, 2617, 2618, 2779, 3275
AsuC2I CCSGG 4 cut(s) 397, 595, 866, 1372
AsuII TTCGAA 1 cut(s) 8
AsuNHI GCTAGC 1 cut(s) 806
AvaII GGWCC 3 cut(s) 107, 726, 3275
AxyI CCTNAGG 1 cut(s) 1131
BaeGI GKGCMC 3 cut(s) 1855, 1987, 2621
BamHI GGATCC 1 cut(s) 1471
BanI GGYRCC 4 cut(s) 200, 861, 1772, 3222
BanII GRGCYC 2 cut(s) 2621, 3323
BarI GAAGNNNNNNTAC 2 cut(s) 1899, 1931
BbsI GAAGAC 1 cut(s) 2923
Bbv12I GWGCWC 5 cut(s) 1855, 1987, 2259, 2398, 3323
BbvI GCAGC 6 cut(s) 703, 778, 992, 2111, 2431, 3037
BccI CCATC 7 cut(s) 329, 401, 2541, 2575, 2856, 3037, 3343
BceAI ACGGC 4 cut(s) 984, 993, 1585, 3318
BcgI CGANNNNNNTGC 6 cut(s) 698, 732, 899, 933, 2510, 2544
BciT130I CCWGG 8 cut(s) 657, 923, 1469, 1476, 1596, 2046, 2802, 3169
BciVI GTATCC 4 cut(s) 28, 460, 663, 2462
BclI TGATCA 3 cut(s) 294, 889, 1216
BcnI CCSGG 4 cut(s) 397, 595, 866, 1372
BcoDI GTCTC 3 cut(s) 847, 1086, 2785
BfmI CTRYAG 5 cut(s) 183, 1137, 1687, 2097, 2925
BfrI CTTAAG 3 cut(s) 1727, 2855, 2959
BfuAI ACCTGC 4 cut(s) 2008, 2569, 2908, 3028
BfuI GTATCC 4 cut(s) 28, 460, 663, 2462
BglI GCCNNNNNGGC 2 cut(s) 248, 977
BglII AGATCT 1 cut(s) 1361
BlpI GCTNAGC 1 cut(s) 1821
BmcAI AGTACT 2 cut(s) 631, 2357
Bme18I GGWCC 3 cut(s) 107, 726, 3275
BmgT120I GGNCC 8 cut(s) 107, 726, 988, 1598, 2617, 2618, 2779, 3275
BmrI ACTGGG 1 cut(s) 423
BmsI GCATC 7 cut(s) 229, 244, 1522, 1841, 2706, 3124, 3314
BmtI GCTAGC 1 cut(s) 810
BmuI ACTGGG 1 cut(s) 423
BpiI GAAGAC 1 cut(s) 2923
BplI GAGNNNNNCTC 2 cut(s) 2129, 2161
BpmI CTGGAG 3 cut(s) 1497, 2871, 3063
Bpu10I CCTNAGC 1 cut(s) 984
Bpu1102I GCTNAGC 1 cut(s) 1821
Bpu14I TTCGAA 1 cut(s) 8
BpuMI CCSGG 4 cut(s) 397, 595, 866, 1372
Bsa29I ATCGAT 1 cut(s) 2520
BsaHI GRCGYC 2 cut(s) 141, 1375
BsaI GGTCTC 3 cut(s) 847, 1086, 2785
BsaWI WCCGGW 2 cut(s) 197, 788
BsaXI ACNNNNNCTCC 6 cut(s) 743, 773, 1330, 1360, 1602, 1632
Bse118I RCCGGY 1 cut(s) 1228
Bse1I ACTGG 6 cut(s) 418, 820, 1953, 2189, 3013, 3437
Bse21I CCTNAGG 1 cut(s) 1131
Bse3DI GCAATG 6 cut(s) 337, 394, 692, 1852, 2658, 2903
BseBI CCWGG 8 cut(s) 657, 923, 1469, 1476, 1596, 2046, 2802, 3169
BseCI ATCGAT 1 cut(s) 2520
BseGI GGATG 9 cut(s) 46, 244, 259, 412, 625, 1896, 2567, 2573, 2657
BseMI GCAATG 6 cut(s) 337, 394, 692, 1852, 2658, 2903
BseNI ACTGG 6 cut(s) 418, 820, 1953, 2189, 3013, 3437
BseRI GAGGAG 4 cut(s) 6, 230, 2453, 2901
BseSI GKGCMC 3 cut(s) 1855, 1987, 2621
BseXI GCAGC 6 cut(s) 703, 778, 992, 2111, 2431, 3037
BseYI CCCAGC 1 cut(s) 831
BsgI GTGCAG 3 cut(s) 1910, 2096, 2547
BshFI GGCC 8 cut(s) 971, 989, 1600, 1995, 2219, 2619, 2781, 3406
BshNI GGYRCC 4 cut(s) 200, 861, 1772, 3222
BshVI ATCGAT 1 cut(s) 2520
BsiHKAI GWGCWC 5 cut(s) 1855, 1987, 2259, 2398, 3323
BslFI GGGAC 6 cut(s) 120, 656, 865, 1308, 3261, 3373
BsmAI GTCTC 3 cut(s) 847, 1086, 2785
BsmFI GGGAC 6 cut(s) 120, 656, 865, 1308, 3261, 3373
BsmI GAATGC 1 cut(s) 3000
BsnI GGCC 8 cut(s) 971, 989, 1600, 1995, 2219, 2619, 2781, 3406
Bso31I GGTCTC 3 cut(s) 847, 1086, 2785
Bsp119I TTCGAA 1 cut(s) 8
Bsp120I GGGCCC 1 cut(s) 2617
Bsp1286I GDGCHC 6 cut(s) 1855, 1987, 2259, 2398, 2621, 3323
Bsp1407I TGTACA 1 cut(s) 3124
Bsp1720I GCTNAGC 1 cut(s) 1821
Bsp19I CCATGG 1 cut(s) 2613
BspANI GGCC 8 cut(s) 971, 989, 1600, 1995, 2219, 2619, 2781, 3406
BspDI ATCGAT 1 cut(s) 2520
BspHI TCATGA 2 cut(s) 1012, 3472
BspMAI CTGCAG 2 cut(s) 2101, 2929
BspMI ACCTGC 4 cut(s) 2008, 2569, 2908, 3028
BspOI GCTAGC 1 cut(s) 810
BspPI GGATC 4 cut(s) 1466, 1479, 2453, 3146
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 4 cut(s) 200, 861, 1772, 3222
BspTI CTTAAG 3 cut(s) 1727, 2855, 2959
BspTNI GGTCTC 3 cut(s) 847, 1086, 2785
BsrDI GCAATG 6 cut(s) 337, 394, 692, 1852, 2658, 2903
BsrFI RCCGGY 1 cut(s) 1228
BsrGI TGTACA 1 cut(s) 3124
BsrI ACTGG 6 cut(s) 418, 820, 1953, 2189, 3013, 3437
BssAI RCCGGY 1 cut(s) 1228
BssNI GRCGYC 2 cut(s) 141, 1375
BssT1I CCWWGG 5 cut(s) 144, 1060, 1788, 2578, 2613
Bst2UI CCWGG 8 cut(s) 657, 923, 1469, 1476, 1596, 2046, 2802, 3169
Bst6I CTCTTC 2 cut(s) 1459, 2329
BstACI GRCGYC 2 cut(s) 141, 1375
BstAFI CTTAAG 3 cut(s) 1727, 2855, 2959
BstAPI GCANNNNNTGC 1 cut(s) 950
BstAUI TGTACA 1 cut(s) 3124
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 8 cut(s) 560, 808, 1483, 1993, 2322, 3000, 3042, 3432
BstDSI CCRYGG 2 cut(s) 966, 2613
BstEII GGTNACC 1 cut(s) 3092
BstENI CCTNNNNNAGG 2 cut(s) 1479, 1905
BstF5I GGATG 9 cut(s) 46, 244, 259, 412, 625, 1896, 2567, 2573, 2657
BstHHI GCGC 2 cut(s) 490, 3436
BstMAI GTCTC 3 cut(s) 847, 1086, 2785
BstNI CCWGG 8 cut(s) 657, 923, 1469, 1476, 1596, 2046, 2802, 3169
BstNSI RCATGY 5 cut(s) 1388, 2320, 3125, 3388, 3434
BstPI GGTNACC 1 cut(s) 3092
BstSFI CTRYAG 5 cut(s) 183, 1137, 1687, 2097, 2925
BstSLI GKGCMC 3 cut(s) 1855, 1987, 2621
BstV1I GCAGC 6 cut(s) 703, 778, 992, 2111, 2431, 3037
BstV2I GAAGAC 1 cut(s) 2923
BstX2I RGATCY 3 cut(s) 1361, 1471, 3151
BstXI CCANNNNNNTGG 2 cut(s) 1475, 2990
BstYI RGATCY 3 cut(s) 1361, 1471, 3151
Bsu15I ATCGAT 1 cut(s) 2520
Bsu36I CCTNAGG 1 cut(s) 1131
BsuI GTATCC 4 cut(s) 28, 460, 663, 2462
BsuRI GGCC 8 cut(s) 971, 989, 1600, 1995, 2219, 2619, 2781, 3406
BsuTUI ATCGAT 1 cut(s) 2520
BtgI CCRYGG 2 cut(s) 966, 2613
BtsCI GGATG 9 cut(s) 46, 244, 259, 412, 625, 1896, 2567, 2573, 2657
BtsI GCAGTG 2 cut(s) 463, 517
BtsIMutI CAGTG 6 cut(s) 411, 463, 517, 1882, 2182, 3444
BveI ACCTGC 4 cut(s) 2008, 2569, 2908, 3028
Cac8I GCNNGC 8 cut(s) 560, 808, 1483, 1993, 2322, 3000, 3042, 3432
CaiI CAGNNNCTG 2 cut(s) 1493, 2153
CciI TCATGA 2 cut(s) 1012, 3472
CfoI GCGC 2 cut(s) 490, 3436
Cfr10I RCCGGY 1 cut(s) 1228
Cfr13I GGNCC 8 cut(s) 107, 726, 988, 1598, 2617, 2618, 2779, 3275
ClaI ATCGAT 1 cut(s) 2520
CseI GACGC 2 cut(s) 149, 1364
Csp6I GTAC 8 cut(s) 630, 862, 904, 1773, 1937, 2356, 3125, 3223
CviQI GTAC 8 cut(s) 630, 862, 904, 1773, 1937, 2356, 3125, 3223
DraIII CACNNNGTG 3 cut(s) 445, 1049, 1888
EaeI YGGCCR 1 cut(s) 969
Eam1104I CTCTTC 2 cut(s) 1459, 2329
EarI CTCTTC 2 cut(s) 1459, 2329
EciI GGCGGA 2 cut(s) 206, 266
Ecl136II GAGCTC 1 cut(s) 3321
Eco130I CCWWGG 5 cut(s) 144, 1060, 1788, 2578, 2613
Eco147I AGGCCT 1 cut(s) 3406
Eco24I GRGCYC 2 cut(s) 2621, 3323
Eco31I GGTCTC 3 cut(s) 847, 1086, 2785
Eco32I GATATC 1 cut(s) 504
Eco47I GGWCC 3 cut(s) 107, 726, 3275
Eco53kI GAGCTC 1 cut(s) 3321
Eco57I CTGAAG 6 cut(s) 822, 1887, 1953, 2346, 2421, 2913
Eco81I CCTNAGG 1 cut(s) 1131
Eco91I GGTNACC 1 cut(s) 3092
EcoICRI GAGCTC 1 cut(s) 3321
EcoNI CCTNNNNNAGG 2 cut(s) 1479, 1905
EcoO109I RGGNCCY 2 cut(s) 107, 2618
EcoO65I GGTNACC 1 cut(s) 3092
EcoRII CCWGG 8 cut(s) 655, 921, 1467, 1474, 1594, 2044, 2800, 3167
EcoRV GATATC 1 cut(s) 504
EcoT14I CCWWGG 5 cut(s) 144, 1060, 1788, 2578, 2613
EcoT22I ATGCAT 2 cut(s) 2721, 3432
EcoT38I GRGCYC 2 cut(s) 2621, 3323
ErhI CCWWGG 5 cut(s) 144, 1060, 1788, 2578, 2613
FalI AAGNNNNNCTT 3 cut(s) 1645, 1677, 3468
FaqI GGGAC 6 cut(s) 120, 656, 865, 1308, 3261, 3373
FauI CCCGC 3 cut(s) 97, 334, 433
FauNDI CATATG 2 cut(s) 873, 1342
FbaI TGATCA 3 cut(s) 294, 889, 1216
FokI GGATG 9 cut(s) 53, 251, 266, 419, 632, 1883, 2554, 2560, 2644
FriOI GRGCYC 2 cut(s) 2621, 3323
FspI TGCGCA 1 cut(s) 489
GlaI GCGC 2 cut(s) 489, 3435
GsaI CCCAGC 1 cut(s) 835
GsuI CTGGAG 3 cut(s) 1497, 2871, 3063
HaeIII GGCC 8 cut(s) 971, 989, 1600, 1995, 2219, 2619, 2781, 3406
HgaI GACGC 2 cut(s) 149, 1364
HhaI GCGC 2 cut(s) 490, 3436
Hin1I GRCGYC 2 cut(s) 141, 1375
Hin6I GCGC 2 cut(s) 488, 3434
HinP1I GCGC 2 cut(s) 488, 3434
HincII GTYRAC 3 cut(s) 1210, 1742, 2971
HindII GTYRAC 3 cut(s) 1210, 1742, 2971
HindIII AAGCTT 2 cut(s) 579, 1823
HpaI GTTAAC 1 cut(s) 1210
Hpy166II GTNNAC 9 cut(s) 625, 700, 1210, 1338, 1742, 1853, 1985, 2590, 2971
Hpy8I GTNNAC 9 cut(s) 625, 700, 1210, 1338, 1742, 1853, 1985, 2590, 2971
Hpy99I CGWCG 6 cut(s) 94, 97, 140, 143, 310, 713
HpyCH4IV ACGT 1 cut(s) 2201
HpySE526I ACGT 1 cut(s) 2201
Hsp92I GRCGYC 2 cut(s) 141, 1375
HspAI GCGC 2 cut(s) 488, 3434
KflI GGGWCCC 1 cut(s) 107
KpnI GGTACC 3 cut(s) 865, 1776, 3226
Ksp22I TGATCA 3 cut(s) 294, 889, 1216
KspAI GTTAAC 1 cut(s) 1210
LmnI GCTCC 9 cut(s) 587, 713, 1351, 1478, 2174, 2547, 3193, 3318, 3326
Lsp1109I GCAGC 6 cut(s) 703, 778, 992, 2111, 2431, 3037
LweI GCATC 7 cut(s) 229, 244, 1522, 1841, 2706, 3124, 3314
MaeII ACGT 1 cut(s) 2201
MaeIII GTNAC 9 cut(s) 733, 796, 1041, 1055, 1393, 1710, 3015, 3092, 3461
MfeI CAATTG 1 cut(s) 915
MflI RGATCY 3 cut(s) 1361, 1471, 3151
MhlI GDGCHC 6 cut(s) 1855, 1987, 2259, 2398, 2621, 3323
MlyI GAGTC 6 cut(s) 290, 332, 623, 1160, 2147, 3170
MmeI TCCRAC 9 cut(s) 159, 288, 597, 1164, 1336, 1373, 1588, 1600, 2311
Mph1103I ATGCAT 2 cut(s) 2721, 3432
MroXI GAANNNNTTC 4 cut(s) 130, 1363, 2338, 2731
MslI CAYNNNNRTG 2 cut(s) 1017, 1424
MspA1I CMGCKG 3 cut(s) 85, 2761, 2891
MspCI CTTAAG 3 cut(s) 1727, 2855, 2959
MunI CAATTG 1 cut(s) 915
Mva1269I GAATGC 1 cut(s) 3000
MvaI CCWGG 8 cut(s) 657, 923, 1469, 1476, 1596, 2046, 2802, 3169
NciI CCSGG 4 cut(s) 397, 595, 866, 1372
NcoI CCATGG 1 cut(s) 2613
NdeI CATATG 2 cut(s) 873, 1342
NheI GCTAGC 1 cut(s) 806
NmuCI GTSAC 5 cut(s) 796, 1041, 3015, 3092, 3461
NsbI TGCGCA 1 cut(s) 489
NsiI ATGCAT 2 cut(s) 2721, 3432
NspI RCATGY 5 cut(s) 1388, 2320, 3125, 3388, 3434
NspV TTCGAA 1 cut(s) 8
PaeI GCATGC 1 cut(s) 3434
PagI TCATGA 2 cut(s) 1012, 3472
PaqCI CACCTGC 2 cut(s) 2569, 3028
PceI AGGCCT 1 cut(s) 3406
PciI ACATGT 1 cut(s) 3121
PctI GAATGC 1 cut(s) 3000
PdmI GAANNNNTTC 4 cut(s) 130, 1363, 2338, 2731
PflFI GACNNNGTC 1 cut(s) 2163
PflMI CCANNNNNTGG 7 cut(s) 1166, 1183, 1355, 2747, 3103, 3250, 3443
PfoI TCCNGGA 1 cut(s) 1474
PleI GAGTC 6 cut(s) 289, 331, 622, 1159, 2146, 3170
PpsI GAGTC 6 cut(s) 289, 331, 622, 1159, 2146, 3170
PpuMI RGGWCCY 1 cut(s) 107
PscI ACATGT 1 cut(s) 3121
PsiI TTATAA 1 cut(s) 1092
Psp124BI GAGCTC 1 cut(s) 3323
Psp5II RGGWCCY 1 cut(s) 107
Psp6I CCWGG 8 cut(s) 655, 921, 1467, 1474, 1594, 2044, 2800, 3167
PspEI GGTNACC 1 cut(s) 3092
PspFI CCCAGC 1 cut(s) 831
PspGI CCWGG 8 cut(s) 655, 921, 1467, 1474, 1594, 2044, 2800, 3167
PspOMI GGGCCC 1 cut(s) 2617
PspPI GGNCC 8 cut(s) 107, 726, 988, 1598, 2617, 2618, 2779, 3275
PspPPI RGGWCCY 1 cut(s) 107
PstI CTGCAG 2 cut(s) 2101, 2929
PstNI CAGNNNCTG 2 cut(s) 1493, 2153
PsuI RGATCY 3 cut(s) 1361, 1471, 3151
PsyI GACNNNGTC 1 cut(s) 2163
PvuII CAGCTG 2 cut(s) 2761, 2891
RsaI GTAC 8 cut(s) 631, 863, 905, 1774, 1938, 2357, 3126, 3224
RsaNI GTAC 8 cut(s) 630, 862, 904, 1773, 1937, 2356, 3125, 3223
RseI CAYNNNNRTG 2 cut(s) 1017, 1424
SacI GAGCTC 1 cut(s) 3323
Sau96I GGNCC 8 cut(s) 107, 726, 988, 1598, 2617, 2618, 2779, 3275
ScaI AGTACT 2 cut(s) 631, 2357
SchI GAGTC 6 cut(s) 290, 332, 623, 1160, 2147, 3170
SduI GDGCHC 6 cut(s) 1855, 1987, 2259, 2398, 2621, 3323
SfaNI GCATC 7 cut(s) 229, 244, 1522, 1841, 2706, 3124, 3314
SfcI CTRYAG 5 cut(s) 183, 1137, 1687, 2097, 2925
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 3 cut(s) 107, 726, 3275
SmiMI CAYNNNNRTG 2 cut(s) 1017, 1424
SmlI CTYRAG 3 cut(s) 1727, 2855, 2959
SmoI CTYRAG 3 cut(s) 1727, 2855, 2959
SphI GCATGC 1 cut(s) 3434
SseBI AGGCCT 1 cut(s) 3406
SspI AATATT 2 cut(s) 2246, 2370
SstI GAGCTC 1 cut(s) 3323
StuI AGGCCT 1 cut(s) 3406
StyI CCWWGG 5 cut(s) 144, 1060, 1788, 2578, 2613
TaiI ACGT 1 cut(s) 2204
TaqI TCGA 9 cut(s) 8, 66, 78, 279, 284, 708, 1242, 2197, 2520
TatI WGTACW 4 cut(s) 629, 1936, 2355, 3124
TauI GCSGC 5 cut(s) 182, 191, 3418, 3450, 3453
TscAI CASTG 6 cut(s) 418, 463, 517, 1889, 2189, 3444
TseFI GTSAC 5 cut(s) 796, 1041, 3015, 3092, 3461
TseI GCWGC 6 cut(s) 716, 766, 980, 2099, 2419, 3050
Tsp45I GTSAC 5 cut(s) 796, 1041, 3015, 3092, 3461
TspGWI ACGGA 2 cut(s) 53, 726
TspRI CASTG 6 cut(s) 418, 463, 517, 1889, 2189, 3444
Tth111I GACNNNGTC 1 cut(s) 2163
Van91I CCANNNNNTGG 7 cut(s) 1166, 1183, 1355, 2747, 3103, 3250, 3443
Vha464I CTTAAG 3 cut(s) 1727, 2855, 2959
VneI GTGCAC 2 cut(s) 1851, 1983
VpaK11BI GGWCC 3 cut(s) 107, 726, 3275
XagI CCTNNNNNAGG 2 cut(s) 1479, 1905
XapI RAATTY 1 cut(s) 2131
XbaI TCTAGA 1 cut(s) 2434
XceI RCATGY 5 cut(s) 1388, 2320, 3125, 3388, 3434
XcmI CCANNNNNNNNNTGG 1 cut(s) 2540
XmnI GAANNNNTTC 4 cut(s) 130, 1363, 2338, 2731
ZrmI AGTACT 2 cut(s) 631, 2357
Zsp2I ATGCAT 2 cut(s) 2721, 3432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.