Rh1AG217000

Protein transport protein sec16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001182
Physical Location & Seq
Forward (+)
41371553 .. 41380779
9227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG217000.1

Sequence Viewer

Length: 4068 bp
ATGGCCAGTTCTGCGTTTGAGTTGGAGGATCAGACGGATGAGGATTTCTTTGACAAATTGATCGATGATGATGACATTGATTTTTCTGGGTATGCAAATAGTTCTGTAGGAAAAGCTGAGCTTGATGTGGTTAAAGATGTTTCGAAATTGAGTATTTCGGATGTAGGTACCGGTGGGAGAACTAGTTCTCAGGTTAATAATGGTGAGTTGGGTCAGAAAGACGGGGTTGTGTTGGAGCCGTCGGATCTGCAGAAGGAGTCTTTGACTGGTGGAGAGAGTGAGTCTAGTGCATTGGTGGCCAAAGGGGAGGAAGGTGTGGTGGATGATACAGTGAGTTCGAGTGTTGTATCAGTTGATGGTGGATTGGGTCGCGGGGATGGGGTTAATGGTCAATTGGGGCACGGTGATGGGGTTGTGTTAGCTTCGTTGGATCGACACCAGGATCCTATAGTCGGTGAAGCGAGCAAGTCATCAGTACCGGAAAATAGAGATGAGGCGGTGGAGGATAAAGCTAGTAATGAGGGTAGTGCATTGGAGGCTAAAGGGGAGGACAGTCCTGTAGATAATACAGTCAGAAATAGTGCGGTGTCAGAGGGAACAGGTGTTAAAGTAGTTCAATGGAGTTCGTTTAACTCAGATCCTTCTGGTGGTAGTGGATTTGGGTCATACTCAGATTTTTTTAGTGAAGTTGGAGATAACTCAGAAGACCCATTTGCAAATTTGGGTAACACAGACCAATCTGGGGTCAAATCTATTGTGTCTAATGGTGTTCTGGAAAGCTCAGTTTCTGCTTTTGGTAATTCTAGCTATGTGCAGCATCAAGAAGACCCATTTGCAAATTTGGGTAATACAGTCCAATTTGGGGCCGAATCTGTTGTGACTAATGGTGTTCTGGAAAATGCTGTTTCTGGTTTTGATACTTTTAGCTATCGGCAGCAACAAGAAGACCCATTTGTAGATTTGGGTAATGCAGACCAATTTGGGGCCAAATCTGCTGTGAGTAATGTTGTTCTGGAAAACTCGGGTTCTGGTTTGGGTGCTTCTATCTATGGGAAGCATCAAGAAGATCCATTTGCGAATTTGGGTAATACAGACCAGTCTGGGGCTGAATCTAATGTGAGTAATGGTGTTTTGGAGAACTCAGTTTCTGGTTTGGGTACTTCTAGCTATGGGCAGCATCAAGAAGGTCAATTCTATGGGGCTCCTGAAAATCAAAACTTAGACGGGCAACAATTAAATAGTAATCAGAATTGGGAAAATCTTTATCCCGGATGGAGGTACGATCCAAATACTGAGCAGTGGTATCCGTTGGAGGGTTATGATGTGAATTCAAACACAAATGCAGAGGTGAACAATAATGCAAATGCTCAAGAGAGTTTTAATGTAAATTCTCAGTCTGCAGATACTGAAGTTTTTTCAGCCCAGAAAGCAGATGCTCCTTATCTGCAGCAAGCAGTGCAGGCTGTTTCTCAAAGCGTTGTTGAGGCATCTGAAACTAGCACAGTTTCTAACTGGAACCAACATTCTTATGGGAATACAGAATACCCTGCACACATGGTTTTTGATCCCCAGTACCCCGGTTGGTACTATGACACAAATATCCAAGAATGGAGGCAGTTGGAACCTTCTGTAGCTACAATCTACCATGATCAGCAAAATACGAGTACATGGCATGCTGAGAATGTTGCCAAGAGTGAAACTATTGGTTTTACTGAGAATCAGGAATGGAGACAGTTGCAGCCTTTCACTCCATCTTTTGATCAGTCAACAACAAGTATGGTTCATAATCAGCAATATAACAATACAAATGTTGACAACCATGGATCACAGAGTCCAATCATTCAACAAAGTGTTTTAAACTGGGATGGATCTATGACCGCCTATGATCAGCAAAATACGAGTTTGTGGCATGCTCAGAAAGTTGCCAAGAGTGATACTGTTGGTTTTACTGAGAAACAGGCATACATGGACCGGCAATTTTCAACTGAGCATGCATTTAATTCTGTGAATCAACAAACAGGGTTCAATCCTTCGGGATCAATTGCTCTTCATAAAGACCCAAGTCAGGGCAATGATATTAGTAGTGCGATTGGTGGCTTTGAAAGGTTTAATTATGCTAACTTTTCTCAGCCTCAGGATCAAGCCAAGGAGCCAAACCAAGCGATGCATTTCTCACCTGCTAACTTTGGTAGTCAGAAGCCAGTACAATTTTTCCAGCAGCCACTCCAGACTGGAACTCAGTTTTCACACGAGGCCAGAGAGGGGAGGTCATCTGCTGGACGACCTGCACATGCTCTGGTTACTTTTGGATTTGGTGGGAAACTTATACTAATGAAAAATAATAGTTCTTTTCATGCAAACCAGACATATGGAAGCCAGGGCTCTGTAGAAGGTGTCATTAATGTTCTCAATCTGATGGATGTTGTCACGGAGAAAAGTGACGCTGCAAACTTTGGAACTGATGGTTGTGATTATTTCAGCGCCTTGTGCCAGCAATCTTTTCCTGGACCTTTAGTTGGTGGGAATGTCGGAAATAAGGAATTAAACAAATGGATTGATGACAAAATTGCAAATTGTGAAACTTCTCACTTGGATTTCAAGAAAGGGGACCATTTGAGATTGCTTTTCTCTTTACTAAAAATATCTTGGCAATTTTATGGAAAACTTCGATCTCCCTTTGGTACCGATCAAAGATTGAAGGACATTGATAATCCAGAATCAGCTCTTGCTAAACTTTTTGCATCTGCTAAAAGAAGTAACGAGTACGGTGCTCTTACCCGGTGCATGCATAACTTGCCTTCTGAAGCACAAATTCAGGCTACTGCTCTTGAGGTACAAAATCTTCTTGTCTCTGGTAGGAAGGATGAGGCTTTACAATGCGCACAAGAAGGTCAATTGTGGGGACCAGCACTTGTTATTGCATCGCAACTTGGTGATCAGTTTTATTGTGATACTGTGAAGCATATGGCTGTAAACCAGTTAATAGCAGGATCACCATTGCGGACGTTGTGCTTGTTAATCGCTAGGCAGCCAGCAGATGTGTTTTCCGATGCTACTACAAATAGCAGTTTTCCTAGTTTCACAAATTTATCACAACGGCACGAACAGGTTGGAGCTAATTACATGTTAGATGACTGGGAAGAGAATCTAGCCATCTTAACTGCAAATAGAACAAAAGATGATGAGCTTGTTATCATCCATCTTGGAGATTCCTTATGGAAGGATAGGGGACAGATAACTGCTGCACACATATGTTATTTAGTTGCAGAAGCCAACCTTGAGCAATATTCGAACAGTTCAAGACTGTGTCTTATCGGTGCTGATCATTGGAAGTTTCCACGAACATACGCCAGTCCAGAGGCTATTCAGAGGACAGAGCTGTATGAATACTCAAAGGTTGTTGGAAATCCTCAGTTTCTTCTACTACCTTTTCAGCCATATAAGCTTATTTATGCCCACATGCTGGCTGAAGTAGGAAAACTTGCTGATGCTTTGAAATATTGTCAAGCAATTTTGAAGTCTTTGAAAACTGCTCGAGCACCTGAGGTGGACACATGGAGAGAGTTGGTGTCATCTCTGGAGGAGCGGATAAGAGCCCATCAGCAGGGCGGTTATAGCATAAATTTGGCTCCAACCAAGTTGATGGGTAAATTGCGTACATTCATTGATAGTACTGCTCATCGTGTTGTTGGGGGGCTACCTGCACCTATACCATCAACAGCACCAGGCTTTCCTCAGCTTAATGAACATTCTCATCAACTACCTCCACCTATACCATCAACAGCACCAGGCTTCCCTCAGCTTAATGAACATGCTCATCAACTACCTCCACCTATACCATCAACAGCACCGGGCTTTGCTCAGCTTAATGAACATGCTCATCAACCAGGAAATCGAAATGTACCCAACAGTCAATCGACAATGGTTATGTCTTCATTAATGCCTTCAGCATCAATGGAGCCAATAAGTGAAAGGACTTCTGAGAGCAACCAACTGAATATGCCTAACAGAAGCATTTCAGAGCCAGACTTTGGGAAAAGCCCGAGTAAGGTAGATACTTGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec16 Sec16_C

Protein Analysis

1355

Amino Acids

147.56

Weight (kDa)

4.69

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec16 PF12932 764 - 887 2.7e-20 Vesicle coat trafficking protein Sec16 mid-region
TPR_Sec16 PF12931 945 - 1232 1.5e-55 Sec16 Sec23-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2185
Acc16I TGCGCA 1 cut(s) 2881
Acc36I ACCTGC 3 cut(s) 2185, 2293, 3709
Acc65I GGTACC 2 cut(s) 167, 2681
AccB1I GGYRCC 2 cut(s) 167, 2681
AccB7I CCANNNNNTGG 2 cut(s) 3463, 4031
AccBSI CCGCTC 1 cut(s) 3586
AccII CGCG 1 cut(s) 372
AciI CCGC 7 cut(s) 372, 497, 584, 1878, 3001, 3586, 3609
AcoI YGGCCR 2 cut(s) 3, 297
AcsI RAATTY 8 cut(s) 718, 838, 1078, 1327, 1387, 2811, 3085, 3622
AcuI CTGAAG 4 cut(s) 1428, 2823, 3489, 3930
AflIII ACRYGT 1 cut(s) 3123
AgeI ACCGGT 1 cut(s) 170
AhlI ACTAGT 1 cut(s) 182
AjnI CCWGG 6 cut(s) 438, 2376, 2503, 3724, 3787, 3886
AloI GAACNNNNNNTCC 2 cut(s) 169, 201
Alw21I GWGCWC 2 cut(s) 2773, 3541
Alw26I GTCTC 2 cut(s) 1723, 2854
AlwNI CAGNNNCTG 3 cut(s) 788, 1148, 1406
Ama87I CYCGRG 3 cut(s) 1021, 3534, 4042
AoxI GGCC 5 cut(s) 3, 297, 864, 984, 2253
ApeKI GCWGC 9 cut(s) 814, 934, 1174, 1447, 1738, 2218, 2444, 3028, 3242
ApoI RAATTY 8 cut(s) 718, 838, 1078, 1327, 1387, 2811, 3085, 3622
AseI ATTAAT 2 cut(s) 2400, 3938
AsiGI ACCGGT 1 cut(s) 170
Asp700I GAANNNNTTC 2 cut(s) 184, 4015
Asp718I GGTACC 2 cut(s) 167, 2681
AspLEI GCGC 2 cut(s) 2483, 2882
AspS9I GGNCC 6 cut(s) 864, 984, 1969, 2507, 2608, 2903
AsuC2I CCSGG 4 cut(s) 1269, 1578, 2779, 3852
AsuHPI GGTGA 7 cut(s) 215, 416, 467, 1360, 2166, 2945, 2985
AsuII TTCGAA 2 cut(s) 143, 3290
AvaI CYCGRG 3 cut(s) 1021, 3534, 4042
AvaII GGWCC 4 cut(s) 1969, 2507, 2608, 2903
AxyI CCTNAGG 2 cut(s) 2133, 3543
BaeGI GKGCMC 1 cut(s) 402
BaeI ACNNNNGTAYC 2 cut(s) 1395, 1428
BalI TGGCCA 2 cut(s) 5, 299
BamHI GGATCC 1 cut(s) 442
BanI GGYRCC 2 cut(s) 167, 2681
BanII GRGCYC 3 cut(s) 1204, 2384, 3598
BarI GAAGNNNNNNTAC 2 cut(s) 2826, 2858
BauI CACGAG 1 cut(s) 2249
BbsI GAAGAC 4 cut(s) 711, 831, 951, 3924
Bbv12I GWGCWC 2 cut(s) 2773, 3541
BbvCI CCTCAGC 2 cut(s) 3735, 3798
BbvI GCAGC 9 cut(s) 826, 946, 1186, 1459, 1750, 2230, 2431, 3040, 3229
BceAI ACGGC 2 cut(s) 223, 3113
BcgI CGANNNNNNTGC 2 cut(s) 2750, 2784
BciT130I CCWGG 6 cut(s) 440, 2378, 2505, 3726, 3789, 3888
BciVI GTATCC 1 cut(s) 1314
BclI TGATCA 5 cut(s) 1648, 1759, 1885, 2935, 3322
BcnI CCSGG 4 cut(s) 1269, 1578, 2779, 3852
BcoDI GTCTC 2 cut(s) 1723, 2854
BcuI ACTAGT 1 cut(s) 182
BfaI CTAG 9 cut(s) 183, 285, 513, 804, 1164, 1497, 3024, 3075, 3149
BfmI CTRYAG 8 cut(s) 105, 248, 447, 558, 1398, 1445, 1629, 2385
BfoI RGCGCY 1 cut(s) 2484
BfuAI ACCTGC 3 cut(s) 2185, 2293, 3709
BfuI GTATCC 1 cut(s) 1314
BisI GCNGC 9 cut(s) 815, 935, 1175, 1448, 1739, 2219, 2445, 3029, 3243
BlpI GCTNAGC 2 cut(s) 117, 3861
BlsI GCNGC 9 cut(s) 816, 936, 1176, 1449, 1740, 2220, 2446, 3030, 3244
BmcAI AGTACT 1 cut(s) 3673
Bme18I GGWCC 4 cut(s) 1969, 2507, 2608, 2903
BmeT110I CYCGRG 3 cut(s) 1021, 3534, 4042
BmgT120I GGNCC 6 cut(s) 864, 984, 1969, 2507, 2608, 2903
BmrI ACTGGG 3 cut(s) 1564, 1870, 3145
BmuI ACTGGG 3 cut(s) 1564, 1870, 3145
BoxI GACNNNNGTC 1 cut(s) 2061
BpiI GAAGAC 4 cut(s) 711, 831, 951, 3924
BpmI CTGGAG 2 cut(s) 2210, 3599
Bpu10I CCTNAGC 2 cut(s) 3735, 3798
Bpu1102I GCTNAGC 2 cut(s) 117, 3861
Bpu14I TTCGAA 2 cut(s) 143, 3290
BpuEI CTTGAG 3 cut(s) 1353, 2849, 3299
BpuMI CCSGG 4 cut(s) 1269, 1578, 2779, 3852
Bsa29I ATCGAT 1 cut(s) 63
BsaJI CCNNGG 4 cut(s) 1576, 1819, 2145, 2377
BsaWI WCCGGW 2 cut(s) 170, 478
BsaXI ACNNNNNCTCC 2 cut(s) 169, 199
Bse118I RCCGGY 2 cut(s) 170, 1971
Bse21I CCTNAGG 2 cut(s) 2133, 3543
Bse3DI GCAATG 2 cut(s) 2077, 2996
BseBI CCWGG 6 cut(s) 440, 2378, 2505, 3726, 3789, 3888
BseCI ATCGAT 1 cut(s) 63
BseDI CCNNGG 4 cut(s) 1576, 1819, 2145, 2377
BseGI GGATG 9 cut(s) 43, 166, 328, 382, 1277, 1870, 2425, 2869, 3195
BseMI GCAATG 2 cut(s) 2077, 2996
BseRI GAGGAG 1 cut(s) 3596
BseSI GKGCMC 1 cut(s) 402
BseXI GCAGC 9 cut(s) 826, 946, 1186, 1459, 1750, 2230, 2431, 3040, 3229
BsgI GTGCAG 6 cut(s) 833, 1478, 1533, 2271, 3228, 3687
Bsh1236I CGCG 1 cut(s) 372
BshFI GGCC 5 cut(s) 5, 299, 866, 986, 2255
BshNI GGYRCC 2 cut(s) 167, 2681
BshTI ACCGGT 1 cut(s) 170
BshVI ATCGAT 1 cut(s) 63
BsiHKAI GWGCWC 2 cut(s) 2773, 3541
BsiHKCI CYCGRG 3 cut(s) 1021, 3534, 4042
BsiSI CCGG 7 cut(s) 171, 479, 1269, 1578, 1972, 2779, 3851
BslFI GGGAC 3 cut(s) 2621, 2916, 3243
BsmAI GTCTC 2 cut(s) 1723, 2854
BsmFI GGGAC 3 cut(s) 2621, 2916, 3243
BsnI GGCC 5 cut(s) 5, 299, 866, 986, 2255
BsoBI CYCGRG 3 cut(s) 1021, 3534, 4042
Bsp119I TTCGAA 2 cut(s) 143, 3290
Bsp1286I GDGCHC 6 cut(s) 402, 1204, 2384, 2773, 3541, 3598
Bsp1720I GCTNAGC 2 cut(s) 117, 3861
Bsp19I CCATGG 1 cut(s) 1819
BspACI CCGC 7 cut(s) 372, 497, 584, 1878, 3001, 3586, 3609
BspANI GGCC 5 cut(s) 5, 299, 866, 986, 2255
BspDI ATCGAT 1 cut(s) 63
BspFNI CGCG 1 cut(s) 372
BspMAI CTGCAG 3 cut(s) 252, 1402, 1449
BspMI ACCTGC 3 cut(s) 2185, 2293, 3709
BspQI GCTCTTC 1 cut(s) 2052
BspT104I TTCGAA 2 cut(s) 143, 3290
BspT107I GGYRCC 2 cut(s) 167, 2681
BsrBI CCGCTC 1 cut(s) 3586
BsrDI GCAATG 2 cut(s) 2077, 2996
BsrFI RCCGGY 2 cut(s) 170, 1971
BssAI RCCGGY 2 cut(s) 170, 1971
BssECI CCNNGG 4 cut(s) 1576, 1819, 2145, 2377
BssSI CACGAG 1 cut(s) 2249
BssT1I CCWWGG 2 cut(s) 1819, 2145
Bst2BI CACGAG 1 cut(s) 2249
Bst2UI CCWGG 6 cut(s) 440, 2378, 2505, 3726, 3789, 3888
Bst6I CTCTTC 2 cut(s) 2052, 3135
BstAPI GCANNNNNTGC 2 cut(s) 1456, 2794
BstBI TTCGAA 2 cut(s) 143, 3290
BstDSI CCRYGG 1 cut(s) 1819
BstF5I GGATG 9 cut(s) 43, 166, 328, 382, 1277, 1870, 2425, 2869, 3195
BstFNI CGCG 1 cut(s) 372
BstH2I RGCGCY 1 cut(s) 2484
BstHHI GCGC 2 cut(s) 2483, 2882
BstMAI GTCTC 2 cut(s) 1723, 2854
BstNI CCWGG 6 cut(s) 440, 2378, 2505, 3726, 3789, 3888
BstNSI RCATGY 9 cut(s) 1676, 1913, 1994, 2294, 2788, 3127, 3463, 3815, 3878
BstPAI GACNNNNGTC 1 cut(s) 2061
BstSFI CTRYAG 8 cut(s) 105, 248, 447, 558, 1398, 1445, 1629, 2385
BstSLI GKGCMC 1 cut(s) 402
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 9 cut(s) 826, 946, 1186, 1459, 1750, 2230, 2431, 3040, 3229
BstV2I GAAGAC 4 cut(s) 711, 831, 951, 3924
BstX2I RGATCY 5 cut(s) 244, 442, 637, 1066, 1868
BstXI CCANNNNNNTGG 2 cut(s) 2369, 3643
BstYI RGATCY 5 cut(s) 244, 442, 637, 1066, 1868
Bsu15I ATCGAT 1 cut(s) 63
Bsu36I CCTNAGG 2 cut(s) 2133, 3543
BsuI GTATCC 1 cut(s) 1314
BsuRI GGCC 5 cut(s) 5, 299, 866, 986, 2255
BsuTUI ATCGAT 1 cut(s) 63
BtgI CCRYGG 1 cut(s) 1819
BtgZI GCGATG 2 cut(s) 2177, 2907
BtsCI GGATG 9 cut(s) 43, 166, 328, 382, 1277, 1870, 2425, 2869, 3195
BtsI GCAGTG 2 cut(s) 1304, 1461
BtsIMutI CAGTG 3 cut(s) 336, 1304, 1461
BveI ACCTGC 3 cut(s) 2185, 2293, 3709
CaiI CAGNNNCTG 3 cut(s) 788, 1148, 1406
CfoI GCGC 2 cut(s) 2483, 2882
Cfr10I RCCGGY 2 cut(s) 170, 1971
Cfr13I GGNCC 6 cut(s) 864, 984, 1969, 2507, 2608, 2903
ClaI ATCGAT 1 cut(s) 63
CseI GACGC 1 cut(s) 2450
CspAI ACCGGT 1 cut(s) 170
DraI TTTAAA 1 cut(s) 1857
EaeI YGGCCR 2 cut(s) 3, 297
Eam1104I CTCTTC 2 cut(s) 2052, 3135
EarI CTCTTC 2 cut(s) 2052, 3135
Eco130I CCWWGG 2 cut(s) 1819, 2145
Eco24I GRGCYC 3 cut(s) 1204, 2384, 3598
Eco47I GGWCC 4 cut(s) 1969, 2507, 2608, 2903
Eco57I CTGAAG 4 cut(s) 1428, 2823, 3489, 3930
Eco81I CCTNAGG 2 cut(s) 2133, 3543
Eco88I CYCGRG 3 cut(s) 1021, 3534, 4042
EcoRI GAATTC 1 cut(s) 1327
EcoRII CCWGG 6 cut(s) 438, 2376, 2503, 3724, 3787, 3886
EcoT14I CCWWGG 2 cut(s) 1819, 2145
EcoT22I ATGCAT 3 cut(s) 1996, 2169, 2790
EcoT38I GRGCYC 3 cut(s) 1204, 2384, 3598
ErhI CCWWGG 2 cut(s) 1819, 2145
FalI AAGNNNNNCTT 4 cut(s) 105, 137, 3261, 3293
FaqI GGGAC 3 cut(s) 2621, 2916, 3243
FauI CCCGC 1 cut(s) 365
FauNDI CATATG 3 cut(s) 2368, 2964, 3251
FbaI TGATCA 5 cut(s) 1648, 1759, 1885, 2935, 3322
Fnu4HI GCNGC 9 cut(s) 815, 935, 1175, 1448, 1739, 2219, 2445, 3029, 3243
FokI GGATG 9 cut(s) 50, 173, 335, 389, 1284, 1877, 2432, 2876, 3182
FriOI GRGCYC 3 cut(s) 1204, 2384, 3598
Fsp4HI GCNGC 9 cut(s) 815, 935, 1175, 1448, 1739, 2219, 2445, 3029, 3243
FspAI RTGCGCAY 1 cut(s) 2881
FspBI CTAG 9 cut(s) 183, 285, 513, 804, 1164, 1497, 3024, 3075, 3149
FspI TGCGCA 1 cut(s) 2881
GlaI GCGC 2 cut(s) 2482, 2881
GluI GCNGC 9 cut(s) 815, 935, 1175, 1448, 1739, 2219, 2445, 3029, 3243
GsuI CTGGAG 2 cut(s) 2210, 3599
HaeII RGCGCY 1 cut(s) 2484
HaeIII GGCC 5 cut(s) 5, 299, 866, 986, 2255
HapII CCGG 7 cut(s) 171, 479, 1269, 1578, 1972, 2779, 3851
HgaI GACGC 1 cut(s) 2450
HhaI GCGC 2 cut(s) 2483, 2882
Hin6I GCGC 2 cut(s) 2481, 2880
HinP1I GCGC 2 cut(s) 2481, 2880
HincII GTYRAC 2 cut(s) 1767, 1813
HindII GTYRAC 2 cut(s) 1767, 1813
HindIII AAGCTT 1 cut(s) 3443
HpaII CCGG 7 cut(s) 171, 479, 1269, 1578, 1972, 2779, 3851
HphI GGTGA 7 cut(s) 215, 416, 467, 1360, 2166, 2945, 2985
Hpy166II GTNNAC 5 cut(s) 1351, 1767, 1813, 2974, 3550
Hpy8I GTNNAC 5 cut(s) 1351, 1767, 1813, 2974, 3550
Hpy99I CGWCG 1 cut(s) 244
HpyCH4IV ACGT 1 cut(s) 3005
HpySE526I ACGT 1 cut(s) 3005
HspAI GCGC 2 cut(s) 2481, 2880
KpnI GGTACC 2 cut(s) 171, 2685
Ksp22I TGATCA 5 cut(s) 1648, 1759, 1885, 2935, 3322
LguI GCTCTTC 1 cut(s) 2052
LmnI GCTCC 8 cut(s) 235, 1207, 1441, 2149, 3113, 3583, 3634, 3958
Lsp1109I GCAGC 9 cut(s) 826, 946, 1186, 1459, 1750, 2230, 2431, 3040, 3229
MaeI CTAG 9 cut(s) 183, 285, 513, 804, 1164, 1497, 3024, 3075, 3149
MaeII ACGT 1 cut(s) 3005
MaeIII GTNAC 6 cut(s) 725, 877, 2299, 2425, 2438, 2756
MbiI CCGCTC 1 cut(s) 3586
MboII GAAGA 9 cut(s) 716, 836, 956, 1076, 2039, 2834, 3152, 3410, 3924
MfeI CAATTG 3 cut(s) 392, 2040, 2894
MflI RGATCY 5 cut(s) 244, 442, 637, 1066, 1868
MhlI GDGCHC 6 cut(s) 402, 1204, 2384, 2773, 3541, 3598
MlsI TGGCCA 2 cut(s) 5, 299
MluNI TGGCCA 2 cut(s) 5, 299
MlyI GAGTC 3 cut(s) 266, 290, 1840
Mox20I TGGCCA 2 cut(s) 5, 299
Mph1103I ATGCAT 3 cut(s) 1996, 2169, 2790
MroXI GAANNNNTTC 2 cut(s) 184, 4015
MscI TGGCCA 2 cut(s) 5, 299
MslI CAYNNNNRTG 3 cut(s) 405, 1527, 3250
Msp20I TGGCCA 2 cut(s) 5, 299
MspI CCGG 7 cut(s) 171, 479, 1269, 1578, 1972, 2779, 3851
MunI CAATTG 3 cut(s) 392, 2040, 2894
MvaI CCWGG 6 cut(s) 440, 2378, 2505, 3726, 3789, 3888
MvnI CGCG 1 cut(s) 372
NciI CCSGG 4 cut(s) 1269, 1578, 2779, 3852
NcoI CCATGG 1 cut(s) 1819
NdeI CATATG 3 cut(s) 2368, 2964, 3251
NmuCI GTSAC 3 cut(s) 877, 2425, 2438
NsbI TGCGCA 1 cut(s) 2881
NsiI ATGCAT 3 cut(s) 1996, 2169, 2790
NspI RCATGY 9 cut(s) 1676, 1913, 1994, 2294, 2788, 3127, 3463, 3815, 3878
NspV TTCGAA 2 cut(s) 143, 3290
PaeI GCATGC 4 cut(s) 1676, 1913, 1994, 2788
PaeR7I CTCGAG 1 cut(s) 3534
PaqCI CACCTGC 1 cut(s) 2185
PciI ACATGT 1 cut(s) 3123
PciSI GCTCTTC 1 cut(s) 2052
PdmI GAANNNNTTC 2 cut(s) 184, 4015
PfeI GAWTC 7 cut(s) 869, 1109, 1717, 2008, 2717, 3145, 3209
PflFI GACNNNGTC 1 cut(s) 3306
PflMI CCANNNNNTGG 2 cut(s) 3463, 4031
PfoI TCCNGGA 2 cut(s) 1267, 2503
PinAI ACCGGT 1 cut(s) 170
PkrI GCNGC 9 cut(s) 816, 936, 1176, 1449, 1740, 2220, 2446, 3030, 3244
PleI GAGTC 3 cut(s) 265, 289, 1839
PpsI GAGTC 3 cut(s) 265, 289, 1839
PscI ACATGT 1 cut(s) 3123
PshAI GACNNNNGTC 1 cut(s) 2061
PshBI ATTAAT 2 cut(s) 2400, 3938
Psp6I CCWGG 6 cut(s) 438, 2376, 2503, 3724, 3787, 3886
PspGI CCWGG 6 cut(s) 438, 2376, 2503, 3724, 3787, 3886
PspPI GGNCC 6 cut(s) 864, 984, 1969, 2507, 2608, 2903
PspXI VCTCGAGB 1 cut(s) 3534
PstI CTGCAG 3 cut(s) 252, 1402, 1449
PstNI CAGNNNCTG 3 cut(s) 788, 1148, 1406
PsuI RGATCY 5 cut(s) 244, 442, 637, 1066, 1868
PsyI GACNNNGTC 1 cut(s) 3306
RseI CAYNNNNRTG 3 cut(s) 405, 1527, 3250
SapI GCTCTTC 1 cut(s) 2052
SatI GCNGC 9 cut(s) 815, 935, 1175, 1448, 1739, 2219, 2445, 3029, 3243
Sau96I GGNCC 6 cut(s) 864, 984, 1969, 2507, 2608, 2903
ScaI AGTACT 1 cut(s) 3673
SchI GAGTC 3 cut(s) 266, 290, 1840
SduI GDGCHC 6 cut(s) 402, 1204, 2384, 2773, 3541, 3598
SfcI CTRYAG 8 cut(s) 105, 248, 447, 558, 1398, 1445, 1629, 2385
Sfr274I CTCGAG 1 cut(s) 3534
SfuI TTCGAA 2 cut(s) 143, 3290
SinI GGWCC 4 cut(s) 1969, 2507, 2608, 2903
SlaI CTCGAG 1 cut(s) 3534
SmiMI CAYNNNNRTG 3 cut(s) 405, 1527, 3250
SmlI CTYRAG 4 cut(s) 1368, 2828, 3278, 3534
SmoI CTYRAG 4 cut(s) 1368, 2828, 3278, 3534
SpeI ACTAGT 1 cut(s) 182
SphI GCATGC 4 cut(s) 1676, 1913, 1994, 2788
SsiI CCGC 7 cut(s) 372, 497, 584, 1878, 3001, 3586, 3609
SspI AATATT 2 cut(s) 3287, 3500
SspMI CTAG 9 cut(s) 183, 285, 513, 804, 1164, 1497, 3024, 3075, 3149
StyI CCWWGG 2 cut(s) 1819, 2145
TaiI ACGT 1 cut(s) 3008
TaqI TCGA 9 cut(s) 63, 143, 338, 433, 2668, 3290, 3535, 3895, 3917
TatI WGTACW 3 cut(s) 1664, 2203, 3671
TfiI GAWTC 7 cut(s) 869, 1109, 1717, 2008, 2717, 3145, 3209
TscAI CASTG 3 cut(s) 336, 1304, 1461
TseFI GTSAC 3 cut(s) 877, 2425, 2438
TseI GCWGC 9 cut(s) 814, 934, 1174, 1447, 1738, 2218, 2444, 3028, 3242
Tsp45I GTSAC 3 cut(s) 877, 2425, 2438
TspGWI ACGGA 3 cut(s) 50, 1296, 2444
TspRI CASTG 3 cut(s) 336, 1304, 1461
Tth111I GACNNNGTC 1 cut(s) 3306
Van91I CCANNNNNTGG 2 cut(s) 3463, 4031
VpaK11BI GGWCC 4 cut(s) 1969, 2507, 2608, 2903
VspI ATTAAT 2 cut(s) 2400, 3938
XapI RAATTY 8 cut(s) 718, 838, 1078, 1327, 1387, 2811, 3085, 3622
XceI RCATGY 9 cut(s) 1676, 1913, 1994, 2294, 2788, 3127, 3463, 3815, 3878
XcmI CCANNNNNNNNNTGG 1 cut(s) 1526
XhoI CTCGAG 1 cut(s) 3534
XmnI GAANNNNTTC 2 cut(s) 184, 4015
XspI CTAG 9 cut(s) 183, 285, 513, 804, 1164, 1497, 3024, 3075, 3149
ZrmI AGTACT 1 cut(s) 3673
Zsp2I ATGCAT 3 cut(s) 1996, 2169, 2790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.