Protein transport protein sec16
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2182 |
| Acc16I | TGCGCA | 1 cut(s) | 2878 |
| Acc36I | ACCTGC | 2 cut(s) | 2182, 2290 |
| Acc65I | GGTACC | 2 cut(s) | 167, 2678 |
| AccB1I | GGYRCC | 2 cut(s) | 167, 2678 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 3460, 4028, 4565 |
| AccBSI | CCGCTC | 1 cut(s) | 3583 |
| AccI | GTMKAC | 1 cut(s) | 4686 |
| AccII | CGCG | 2 cut(s) | 369, 4111 |
| AciI | CCGC | 7 cut(s) | 369, 1875, 2998, 3583, 4289, 4421, 4703 |
| AclI | AACGTT | 1 cut(s) | 917 |
| AcoI | YGGCCR | 4 cut(s) | 3, 294, 3462, 4169 |
| AcuI | CTGAAG | 4 cut(s) | 1425, 2820, 3927, 4653 |
| AcvI | CACGTG | 1 cut(s) | 4457 |
| AflIII | ACRYGT | 1 cut(s) | 3120 |
| AgeI | ACCGGT | 1 cut(s) | 170 |
| AhlI | ACTAGT | 2 cut(s) | 182, 4355 |
| AjnI | CCWGG | 8 cut(s) | 435, 2373, 2500, 3020, 3721, 3784, 4516, 4558 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 169, 201 |
| Alw21I | GWGCWC | 3 cut(s) | 2770, 3538, 4420 |
| Alw26I | GTCTC | 3 cut(s) | 266, 1720, 2851 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 785, 1145, 1403 |
| Ama87I | CYCGRG | 3 cut(s) | 1018, 3531, 4039 |
| AoxI | GGCC | 8 cut(s) | 3, 294, 861, 981, 2250, 3462, 4169, 4376 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 978, 1010 |
| AseI | ATTAAT | 2 cut(s) | 2397, 3935 |
| AsiGI | ACCGGT | 1 cut(s) | 170 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 4012, 4205, 4620 |
| Asp718I | GGTACC | 2 cut(s) | 167, 2678 |
| AspLEI | GCGC | 2 cut(s) | 2480, 2879 |
| AspS9I | GGNCC | 8 cut(s) | 386, 861, 981, 1966, 2504, 2605, 2900, 4376 |
| AsuC2I | CCSGG | 4 cut(s) | 1266, 1575, 3849, 4168 |
| AsuHPI | GGTGA | 8 cut(s) | 212, 464, 536, 1357, 2163, 2942, 2982, 4205 |
| AsuII | TTCGAA | 2 cut(s) | 143, 3287 |
| AvaI | CYCGRG | 3 cut(s) | 1018, 3531, 4039 |
| AvaII | GGWCC | 5 cut(s) | 386, 1966, 2504, 2605, 2900 |
| AxyI | CCTNAGG | 1 cut(s) | 3540 |
| BaeGI | GKGCMC | 1 cut(s) | 399 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1392, 1425 |
| BalI | TGGCCA | 2 cut(s) | 5, 296 |
| BamHI | GGATCC | 1 cut(s) | 439 |
| BanI | GGYRCC | 2 cut(s) | 167, 2678 |
| BanII | GRGCYC | 3 cut(s) | 1201, 2381, 3595 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 2823, 2855, 4260, 4292 |
| BauI | CACGAG | 1 cut(s) | 2246 |
| BbrPI | CACGTG | 1 cut(s) | 4457 |
| BbsI | GAAGAC | 4 cut(s) | 708, 828, 948, 3921 |
| Bbv12I | GWGCWC | 3 cut(s) | 2770, 3538, 4420 |
| BbvCI | CCTCAGC | 3 cut(s) | 3732, 3795, 4263 |
| BceAI | ACGGC | 3 cut(s) | 220, 415, 3110 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 2747, 2781, 4229, 4263 |
| BciT130I | CCWGG | 8 cut(s) | 437, 2375, 2502, 3022, 3723, 3786, 4518, 4560 |
| BciVI | GTATCC | 1 cut(s) | 1311 |
| BclI | TGATCA | 5 cut(s) | 1645, 1756, 1882, 2932, 3319 |
| BcnI | CCSGG | 4 cut(s) | 1266, 1575, 3849, 4168 |
| BcoDI | GTCTC | 3 cut(s) | 266, 1720, 2851 |
| BcuI | ACTAGT | 2 cut(s) | 182, 4355 |
| BfmI | CTRYAG | 9 cut(s) | 105, 245, 444, 555, 1395, 1442, 1626, 2442, 4299 |
| BfoI | RGCGCY | 1 cut(s) | 2481 |
| BfuAI | ACCTGC | 2 cut(s) | 2182, 2290 |
| BfuI | GTATCC | 1 cut(s) | 1311 |
| BlpI | GCTNAGC | 1 cut(s) | 3858 |
| BmcAI | AGTACT | 1 cut(s) | 3670 |
| Bme18I | GGWCC | 5 cut(s) | 386, 1966, 2504, 2605, 2900 |
| BmeT110I | CYCGRG | 3 cut(s) | 1018, 3531, 4039 |
| BmgT120I | GGNCC | 8 cut(s) | 386, 861, 981, 1966, 2504, 2605, 2900, 4376 |
| BmrI | ACTGGG | 4 cut(s) | 1561, 1867, 3142, 4428 |
| BmuI | ACTGGG | 4 cut(s) | 1561, 1867, 3142, 4428 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2058 |
| BpiI | GAAGAC | 4 cut(s) | 708, 828, 948, 3921 |
| BpmI | CTGGAG | 4 cut(s) | 2207, 3596, 4242, 4581 |
| Bpu10I | CCTNAGC | 4 cut(s) | 1365, 3732, 3795, 4263 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 3858 |
| Bpu14I | TTCGAA | 2 cut(s) | 143, 3287 |
| BpuEI | CTTGAG | 4 cut(s) | 2846, 3296, 4211, 4462 |
| BpuMI | CCSGG | 4 cut(s) | 1266, 1575, 3849, 4168 |
| Bsa29I | ATCGAT | 1 cut(s) | 63 |
| BsaAI | YACGTR | 2 cut(s) | 1665, 4457 |
| BsaJI | CCNNGG | 6 cut(s) | 1573, 1816, 2142, 2374, 4516, 4559 |
| BsaWI | WCCGGW | 2 cut(s) | 170, 475 |
| Bse118I | RCCGGY | 2 cut(s) | 170, 1968 |
| Bse21I | CCTNAGG | 1 cut(s) | 3540 |
| Bse3DI | GCAATG | 2 cut(s) | 2074, 2993 |
| BseBI | CCWGG | 8 cut(s) | 437, 2375, 2502, 3022, 3723, 3786, 4518, 4560 |
| BseCI | ATCGAT | 1 cut(s) | 63 |
| BseDI | CCNNGG | 6 cut(s) | 1573, 1816, 2142, 2374, 4516, 4559 |
| BseMI | GCAATG | 2 cut(s) | 2074, 2993 |
| BseRI | GAGGAG | 1 cut(s) | 3593 |
| BseSI | GKGCMC | 1 cut(s) | 399 |
| BseX3I | CGGCCG | 1 cut(s) | 4169 |
| BseYI | CCCAGC | 1 cut(s) | 4393 |
| BsgI | GTGCAG | 5 cut(s) | 1475, 1530, 2268, 3225, 4437 |
| Bsh1236I | CGCG | 2 cut(s) | 369, 4111 |
| Bsh1285I | CGRYCG | 1 cut(s) | 4172 |
| BshFI | GGCC | 8 cut(s) | 5, 296, 863, 983, 2252, 3464, 4171, 4378 |
| BshNI | GGYRCC | 2 cut(s) | 167, 2678 |
| BshTI | ACCGGT | 1 cut(s) | 170 |
| BshVI | ATCGAT | 1 cut(s) | 63 |
| BsiEI | CGRYCG | 1 cut(s) | 4172 |
| BsiHKAI | GWGCWC | 3 cut(s) | 2770, 3538, 4420 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1018, 3531, 4039 |
| BsiSI | CCGG | 8 cut(s) | 171, 476, 1266, 1575, 1969, 3848, 4168, 4172 |
| BslFI | GGGAC | 4 cut(s) | 2618, 2913, 3240, 4891 |
| BsmAI | GTCTC | 3 cut(s) | 266, 1720, 2851 |
| BsmFI | GGGAC | 4 cut(s) | 2618, 2913, 3240, 4891 |
| BsmI | GAATGC | 1 cut(s) | 4323 |
| BsnI | GGCC | 8 cut(s) | 5, 296, 863, 983, 2252, 3464, 4171, 4378 |
| BsoBI | CYCGRG | 3 cut(s) | 1018, 3531, 4039 |
| Bsp119I | TTCGAA | 2 cut(s) | 143, 3287 |
| Bsp1286I | GDGCHC | 7 cut(s) | 399, 1201, 2381, 2770, 3538, 3595, 4420 |
| Bsp1407I | TGTACA | 1 cut(s) | 4762 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 3858 |
| Bsp19I | CCATGG | 1 cut(s) | 1816 |
| BspACI | CCGC | 7 cut(s) | 369, 1875, 2998, 3583, 4289, 4421, 4703 |
| BspANI | GGCC | 8 cut(s) | 5, 296, 863, 983, 2252, 3464, 4171, 4378 |
| BspDI | ATCGAT | 1 cut(s) | 63 |
| BspFNI | CGCG | 2 cut(s) | 369, 4111 |
| BspHI | TCATGA | 1 cut(s) | 4984 |
| BspMAI | CTGCAG | 5 cut(s) | 249, 1399, 1446, 2446, 4303 |
| BspMI | ACCTGC | 2 cut(s) | 2182, 2290 |
| BspQI | GCTCTTC | 1 cut(s) | 2049 |
| BspT104I | TTCGAA | 2 cut(s) | 143, 3287 |
| BspT107I | GGYRCC | 2 cut(s) | 167, 2678 |
| BsrBI | CCGCTC | 1 cut(s) | 3583 |
| BsrDI | GCAATG | 2 cut(s) | 2074, 2993 |
| BsrFI | RCCGGY | 2 cut(s) | 170, 1968 |
| BsrGI | TGTACA | 1 cut(s) | 4762 |
| BssAI | RCCGGY | 2 cut(s) | 170, 1968 |
| BssECI | CCNNGG | 6 cut(s) | 1573, 1816, 2142, 2374, 4516, 4559 |
| BssSI | CACGAG | 1 cut(s) | 2246 |
| BssT1I | CCWWGG | 2 cut(s) | 1816, 2142 |
| Bst2BI | CACGAG | 1 cut(s) | 2246 |
| Bst2UI | CCWGG | 8 cut(s) | 437, 2375, 2502, 3022, 3723, 3786, 4518, 4560 |
| Bst6I | CTCTTC | 3 cut(s) | 2049, 3132, 4548 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1453, 2782, 2791 |
| BstAUI | TGTACA | 1 cut(s) | 4762 |
| BstBAI | YACGTR | 2 cut(s) | 1665, 4457 |
| BstBI | TTCGAA | 2 cut(s) | 143, 3287 |
| BstDSI | CCRYGG | 1 cut(s) | 1816 |
| BstFNI | CGCG | 2 cut(s) | 369, 4111 |
| BstH2I | RGCGCY | 1 cut(s) | 2481 |
| BstHHI | GCGC | 2 cut(s) | 2480, 2879 |
| BstMAI | GTCTC | 3 cut(s) | 266, 1720, 2851 |
| BstMCI | CGRYCG | 1 cut(s) | 4172 |
| BstNI | CCWGG | 8 cut(s) | 437, 2375, 2502, 3022, 3723, 3786, 4518, 4560 |
| BstNSI | RCATGY | 9 cut(s) | 1673, 1910, 1991, 2291, 2785, 3124, 3460, 3749, 3875 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2058 |
| BstSFI | CTRYAG | 9 cut(s) | 105, 245, 444, 555, 1395, 1442, 1626, 2442, 4299 |
| BstSLI | GKGCMC | 1 cut(s) | 399 |
| BstUI | CGCG | 2 cut(s) | 369, 4111 |
| BstV2I | GAAGAC | 4 cut(s) | 708, 828, 948, 3921 |
| BstX2I | RGATCY | 6 cut(s) | 241, 439, 634, 1063, 1865, 4163 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 2366, 3605, 3640 |
| BstYI | RGATCY | 6 cut(s) | 241, 439, 634, 1063, 1865, 4163 |
| BstZI | CGGCCG | 1 cut(s) | 4169 |
| Bsu15I | ATCGAT | 1 cut(s) | 63 |
| Bsu36I | CCTNAGG | 1 cut(s) | 3540 |
| BsuI | GTATCC | 1 cut(s) | 1311 |
| BsuRI | GGCC | 8 cut(s) | 5, 296, 863, 983, 2252, 3464, 4171, 4378 |
| BsuTUI | ATCGAT | 1 cut(s) | 63 |
| BtgI | CCRYGG | 1 cut(s) | 1816 |
| BtgZI | GCGATG | 3 cut(s) | 417, 2904, 4246 |
| BtsI | GCAGTG | 6 cut(s) | 501, 1301, 1458, 2783, 4513, 4966 |
| BtsIMutI | CAGTG | 9 cut(s) | 333, 501, 1301, 1458, 1944, 2783, 4343, 4513, 4966 |
| BveI | ACCTGC | 2 cut(s) | 2182, 2290 |
| CaiI | CAGNNNCTG | 3 cut(s) | 785, 1145, 1403 |
| CciI | TCATGA | 1 cut(s) | 4984 |
| CfoI | GCGC | 2 cut(s) | 2480, 2879 |
| Cfr10I | RCCGGY | 2 cut(s) | 170, 1968 |
| Cfr13I | GGNCC | 8 cut(s) | 386, 861, 981, 1966, 2504, 2605, 2900, 4376 |
| ClaI | ATCGAT | 1 cut(s) | 63 |
| CseI | GACGC | 1 cut(s) | 2447 |
| CspAI | ACCGGT | 1 cut(s) | 170 |
| DraI | TTTAAA | 1 cut(s) | 1854 |
| EaeI | YGGCCR | 4 cut(s) | 3, 294, 3462, 4169 |
| EagI | CGGCCG | 1 cut(s) | 4169 |
| Eam1104I | CTCTTC | 3 cut(s) | 2049, 3132, 4548 |
| EarI | CTCTTC | 3 cut(s) | 2049, 3132, 4548 |
| EciI | GGCGGA | 2 cut(s) | 4278, 4692 |
| EclXI | CGGCCG | 1 cut(s) | 4169 |
| Eco130I | CCWWGG | 2 cut(s) | 1816, 2142 |
| Eco24I | GRGCYC | 3 cut(s) | 1201, 2381, 3595 |
| Eco47I | GGWCC | 5 cut(s) | 386, 1966, 2504, 2605, 2900 |
| Eco52I | CGGCCG | 1 cut(s) | 4169 |
| Eco57I | CTGAAG | 4 cut(s) | 1425, 2820, 3927, 4653 |
| Eco72I | CACGTG | 1 cut(s) | 4457 |
| Eco81I | CCTNAGG | 1 cut(s) | 3540 |
| Eco88I | CYCGRG | 3 cut(s) | 1018, 3531, 4039 |
| EcoRI | GAATTC | 2 cut(s) | 1324, 4408 |
| EcoRII | CCWGG | 8 cut(s) | 435, 2373, 2500, 3020, 3721, 3784, 4516, 4558 |
| EcoT14I | CCWWGG | 2 cut(s) | 1816, 2142 |
| EcoT22I | ATGCAT | 3 cut(s) | 1993, 2166, 2787 |
| EcoT38I | GRGCYC | 3 cut(s) | 1201, 2381, 3595 |
| ErhI | CCWWGG | 2 cut(s) | 1816, 2142 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 3258, 3290, 4849, 4881 |
| FaqI | GGGAC | 4 cut(s) | 2618, 2913, 3240, 4891 |
| FauI | CCCGC | 1 cut(s) | 362 |
| FauNDI | CATATG | 3 cut(s) | 2365, 2961, 3248 |
| FbaI | TGATCA | 5 cut(s) | 1645, 1756, 1882, 2932, 3319 |
| FblI | GTMKAC | 1 cut(s) | 4686 |
| FriOI | GRGCYC | 3 cut(s) | 1201, 2381, 3595 |
| FspAI | RTGCGCAY | 1 cut(s) | 2878 |
| FspI | TGCGCA | 1 cut(s) | 2878 |
| GlaI | GCGC | 2 cut(s) | 2479, 2878 |
| GsaI | CCCAGC | 1 cut(s) | 4397 |
| GsuI | CTGGAG | 4 cut(s) | 2207, 3596, 4242, 4581 |
| HaeII | RGCGCY | 1 cut(s) | 2481 |
| HaeIII | GGCC | 8 cut(s) | 5, 296, 863, 983, 2252, 3464, 4171, 4378 |
| HapII | CCGG | 8 cut(s) | 171, 476, 1266, 1575, 1969, 3848, 4168, 4172 |
| HgaI | GACGC | 1 cut(s) | 2447 |
| HhaI | GCGC | 2 cut(s) | 2480, 2879 |
| Hin6I | GCGC | 2 cut(s) | 2478, 2877 |
| HinP1I | GCGC | 2 cut(s) | 2478, 2877 |
| HincII | GTYRAC | 3 cut(s) | 1764, 1810, 3714 |
| HindII | GTYRAC | 3 cut(s) | 1764, 1810, 3714 |
| HindIII | AAGCTT | 3 cut(s) | 2837, 3440, 3860 |
| HpaII | CCGG | 8 cut(s) | 171, 476, 1266, 1575, 1969, 3848, 4168, 4172 |
| HphI | GGTGA | 8 cut(s) | 212, 464, 536, 1357, 2163, 2942, 2982, 4205 |
| Hpy166II | GTNNAC | 8 cut(s) | 1348, 1764, 1810, 2971, 3547, 3714, 4069, 4687 |
| Hpy8I | GTNNAC | 8 cut(s) | 1348, 1764, 1810, 2971, 3547, 3714, 4069, 4687 |
| Hpy99I | CGWCG | 2 cut(s) | 241, 4824 |
| HpyCH4IV | ACGT | 4 cut(s) | 917, 1664, 3002, 4456 |
| HpySE526I | ACGT | 4 cut(s) | 917, 1664, 3002, 4456 |
| HspAI | GCGC | 2 cut(s) | 2478, 2877 |
| KpnI | GGTACC | 2 cut(s) | 171, 2682 |
| Ksp22I | TGATCA | 5 cut(s) | 1645, 1756, 1882, 2932, 3319 |
| LguI | GCTCTTC | 1 cut(s) | 2049 |
| LmnI | GCTCC | 9 cut(s) | 232, 1204, 1438, 2146, 3110, 3580, 3631, 3955, 4415 |
| MaeII | ACGT | 4 cut(s) | 917, 1664, 3002, 4456 |
| MbiI | CCGCTC | 1 cut(s) | 3583 |
| MfeI | CAATTG | 3 cut(s) | 2037, 2085, 2891 |
| MflI | RGATCY | 6 cut(s) | 241, 439, 634, 1063, 1865, 4163 |
| MhlI | GDGCHC | 7 cut(s) | 399, 1201, 2381, 2770, 3538, 3595, 4420 |
| MlsI | TGGCCA | 2 cut(s) | 5, 296 |
| MluNI | TGGCCA | 2 cut(s) | 5, 296 |
| MlyI | GAGTC | 4 cut(s) | 263, 287, 1837, 4138 |
| Mox20I | TGGCCA | 2 cut(s) | 5, 296 |
| Mph1103I | ATGCAT | 3 cut(s) | 1993, 2166, 2787 |
| MroXI | GAANNNNTTC | 3 cut(s) | 4012, 4205, 4620 |
| MscI | TGGCCA | 2 cut(s) | 5, 296 |
| MslI | CAYNNNNRTG | 2 cut(s) | 402, 3247 |
| Msp20I | TGGCCA | 2 cut(s) | 5, 296 |
| MspA1I | CMGCKG | 2 cut(s) | 4262, 4550 |
| MspI | CCGG | 8 cut(s) | 171, 476, 1266, 1575, 1969, 3848, 4168, 4172 |
| MunI | CAATTG | 3 cut(s) | 2037, 2085, 2891 |
| Mva1269I | GAATGC | 1 cut(s) | 4323 |
| MvaI | CCWGG | 8 cut(s) | 437, 2375, 2502, 3022, 3723, 3786, 4518, 4560 |
| MvnI | CGCG | 2 cut(s) | 369, 4111 |
| NciI | CCSGG | 4 cut(s) | 1266, 1575, 3849, 4168 |
| NcoI | CCATGG | 1 cut(s) | 1816 |
| NdeI | CATATG | 3 cut(s) | 2365, 2961, 3248 |
| NmuCI | GTSAC | 5 cut(s) | 874, 2422, 2435, 4125, 4657 |
| NsbI | TGCGCA | 1 cut(s) | 2878 |
| NsiI | ATGCAT | 3 cut(s) | 1993, 2166, 2787 |
| NspI | RCATGY | 9 cut(s) | 1673, 1910, 1991, 2291, 2785, 3124, 3460, 3749, 3875 |
| NspV | TTCGAA | 2 cut(s) | 143, 3287 |
| PaeI | GCATGC | 4 cut(s) | 1673, 1910, 1991, 2785 |
| PaeR7I | CTCGAG | 1 cut(s) | 3531 |
| PagI | TCATGA | 1 cut(s) | 4984 |
| PaqCI | CACCTGC | 1 cut(s) | 2182 |
| PciI | ACATGT | 1 cut(s) | 3120 |
| PciSI | GCTCTTC | 1 cut(s) | 2049 |
| PctI | GAATGC | 1 cut(s) | 4323 |
| PdmI | GAANNNNTTC | 3 cut(s) | 4012, 4205, 4620 |
| PflFI | GACNNNGTC | 2 cut(s) | 277, 3303 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 3460, 4028, 4565 |
| PfoI | TCCNGGA | 2 cut(s) | 1264, 2500 |
| PinAI | ACCGGT | 1 cut(s) | 170 |
| PleI | GAGTC | 4 cut(s) | 262, 286, 1836, 4138 |
| PmaCI | CACGTG | 1 cut(s) | 4457 |
| PmlI | CACGTG | 1 cut(s) | 4457 |
| PpsI | GAGTC | 4 cut(s) | 262, 286, 1836, 4138 |
| Ppu21I | YACGTR | 2 cut(s) | 1665, 4457 |
| PscI | ACATGT | 1 cut(s) | 3120 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2058 |
| PshBI | ATTAAT | 2 cut(s) | 2397, 3935 |
| Psp1406I | AACGTT | 1 cut(s) | 917 |
| Psp6I | CCWGG | 8 cut(s) | 435, 2373, 2500, 3020, 3721, 3784, 4516, 4558 |
| PspCI | CACGTG | 1 cut(s) | 4457 |
| PspFI | CCCAGC | 1 cut(s) | 4393 |
| PspGI | CCWGG | 8 cut(s) | 435, 2373, 2500, 3020, 3721, 3784, 4516, 4558 |
| PspPI | GGNCC | 8 cut(s) | 386, 861, 981, 1966, 2504, 2605, 2900, 4376 |
| PspXI | VCTCGAGB | 1 cut(s) | 3531 |
| PstI | CTGCAG | 5 cut(s) | 249, 1399, 1446, 2446, 4303 |
| PstNI | CAGNNNCTG | 3 cut(s) | 785, 1145, 1403 |
| PsuI | RGATCY | 6 cut(s) | 241, 439, 634, 1063, 1865, 4163 |
| PsyI | GACNNNGTC | 2 cut(s) | 277, 3303 |
| PvuII | CAGCTG | 2 cut(s) | 4262, 4550 |
| RseI | CAYNNNNRTG | 2 cut(s) | 402, 3247 |
| SapI | GCTCTTC | 1 cut(s) | 2049 |
| Sau96I | GGNCC | 8 cut(s) | 386, 861, 981, 1966, 2504, 2605, 2900, 4376 |
| ScaI | AGTACT | 1 cut(s) | 3670 |
| SchI | GAGTC | 4 cut(s) | 263, 287, 1837, 4138 |
| SduI | GDGCHC | 7 cut(s) | 399, 1201, 2381, 2770, 3538, 3595, 4420 |
| SfcI | CTRYAG | 9 cut(s) | 105, 245, 444, 555, 1395, 1442, 1626, 2442, 4299 |
| Sfr274I | CTCGAG | 1 cut(s) | 3531 |
| SfuI | TTCGAA | 2 cut(s) | 143, 3287 |
| SinI | GGWCC | 5 cut(s) | 386, 1966, 2504, 2605, 2900 |
| SlaI | CTCGAG | 1 cut(s) | 3531 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 402, 3247 |
| SmlI | CTYRAG | 5 cut(s) | 2825, 3275, 3531, 4226, 4477 |
| SmoI | CTYRAG | 5 cut(s) | 2825, 3275, 3531, 4226, 4477 |
| SpeI | ACTAGT | 2 cut(s) | 182, 4355 |
| SphI | GCATGC | 4 cut(s) | 1673, 1910, 1991, 2785 |
| SsiI | CCGC | 7 cut(s) | 369, 1875, 2998, 3583, 4289, 4421, 4703 |
| SspI | AATATT | 2 cut(s) | 3284, 3497 |
| StyI | CCWWGG | 2 cut(s) | 1816, 2142 |
| TaiI | ACGT | 4 cut(s) | 920, 1667, 3005, 4459 |
| TatI | WGTACW | 3 cut(s) | 2200, 3668, 4762 |
| TscAI | CASTG | 9 cut(s) | 333, 501, 1301, 1458, 1951, 2783, 4350, 4513, 4966 |
| TseFI | GTSAC | 5 cut(s) | 874, 2422, 2435, 4125, 4657 |
| Tsp45I | GTSAC | 5 cut(s) | 874, 2422, 2435, 4125, 4657 |
| TspGWI | ACGGA | 4 cut(s) | 50, 859, 1293, 2441 |
| TspRI | CASTG | 9 cut(s) | 333, 501, 1301, 1458, 1951, 2783, 4350, 4513, 4966 |
| Tth111I | GACNNNGTC | 2 cut(s) | 277, 3303 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 3460, 4028, 4565 |
| VpaK11BI | GGWCC | 5 cut(s) | 386, 1966, 2504, 2605, 2900 |
| VspI | ATTAAT | 2 cut(s) | 2397, 3935 |
| XceI | RCATGY | 9 cut(s) | 1673, 1910, 1991, 2291, 2785, 3124, 3460, 3749, 3875 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 3602 |
| XhoI | CTCGAG | 1 cut(s) | 3531 |
| XmiI | GTMKAC | 1 cut(s) | 4686 |
| XmnI | GAANNNNTTC | 3 cut(s) | 4012, 4205, 4620 |
| ZrmI | AGTACT | 1 cut(s) | 3670 |
| Zsp2I | ATGCAT | 3 cut(s) | 1993, 2166, 2787 |