Rmu_sc0018299.1_g000001

Protein transport protein sec16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0018299.1
OG0001182
Physical Location & Seq
Forward (+)
1 .. 1637
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0018299.1_g000001.1.cds

Sequence Viewer

Length: 1333 bp
ctctggggaagaaagcaaaaacgagccggatgtaaacagtaccgagtattgggacagtctgtatcctgggtggaagtatgatcccaacacagggcaatggtatgaggtggacagtttcgatggagctgctaattggaccaccgttactgatactaaacaggagatttcgtatttgcagcaaacagctcactctgctgccggaactgtgactgaagctagcactactggttgtttgtctagctgggaacaggtctcacaagggactaatgggtacccggaacatatggttttttatcctgatcaccctgattggtactacgacacaattgcccaggagtggcgttcattggaagcatataatgcttctatgcaacccacggccacaacagcagctcaggcccagaatgagaatagtttatatcatgagtatggacaagctgaaaattatgggtcacaaggtgttagtaaccaaggtcaagagggtagctggtctcagtcttataataattacaatcagcaagtttcaaatatgtggcaacctgaggctgtagctaccagcgagtcttttccaagttttggtggaaaccaacagttgggtaattcattagcttctatggttaacaaggatcagcagaagttcggagcagttcctttgtataacaaagcaagtcagggtcatggtgaggctaatgggactgttgggttccaaagttttgccccaggtggaaactttaaccagcagtttaatcaaggaaatgcaaagcagaatgaacaaacccaattttcagaggattactatggcagtcagaaacctgtaaattattcacaacaatctttccagagtggcaatcagtttacatatgctccaactgttggaagatcttctgccgggcgtcctccacatgccttagtaacttttggatttggtggaaaactcattgtgatgaaggacagtagtagtcttgggaactcttcctatggaagccaggatcctgtagcgggctcagtttctgttctgaacttgacagaagttttcatgcaaaagcctgatgcttcaagttttggaatgactacttgtgattattttcgtgccttgtgccaacagtcctttccagggccgttggttggagggaatgttggaagtaaagagttgaacaaatggattgatgaaaggattgcttactgtgaatcgcccgacatggactataggaaaggaaaagtattgaggttacttatttctttgcttaagatagcttgtcaacactatggaaaactccgatctccttttggtaccgacactctatccaaggtacatcacattcatgtcctttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec16 Sec16_C

Protein Analysis

443

Amino Acids

48.83

Weight (kDa)

4.95

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 502
Acc65I GGTACC 2 cut(s) 271, 1290
AccB1I GGYRCC 2 cut(s) 271, 1290
AccB7I CCANNNNNTGG 3 cut(s) 576, 593, 873
AciI CCGC 1 cut(s) 999
AclWI GGATC 4 cut(s) 75, 634, 984, 997
AcoI YGGCCR 1 cut(s) 379
AcuI CTGAAG 1 cut(s) 232
AcyI GRCGYC 1 cut(s) 893
AdeI CACNNNGTG 1 cut(s) 459
AfaI GTAC 5 cut(s) 41, 273, 315, 1292, 1312
AflII CTTAAG 1 cut(s) 1245
AgsI TTSAA 3 cut(s) 526, 1057, 1154
AjnI CCWGG 5 cut(s) 65, 331, 719, 985, 1112
AjuI GAANNNNNNNTTGG 2 cut(s) 1093, 1125
Alw26I GTCTC 2 cut(s) 257, 496
AlwI GGATC 4 cut(s) 75, 634, 984, 997
AlwNI CAGNNNCTG 1 cut(s) 1011
AoxI GGCC 3 cut(s) 379, 397, 1116
ApeKI GCWGC 4 cut(s) 126, 176, 195, 390
Asp700I GAANNNNTTC 1 cut(s) 881
Asp718I GGTACC 2 cut(s) 271, 1290
AspS9I GGNCC 3 cut(s) 136, 398, 1116
AsuC2I CCSGG 2 cut(s) 276, 890
AsuHPI GGTGA 2 cut(s) 294, 693
AsuNHI GCTAGC 1 cut(s) 216
AvaII GGWCC 1 cut(s) 136
AxyI CCTNAGG 1 cut(s) 541
BamHI GGATCC 1 cut(s) 989
BanI GGYRCC 2 cut(s) 271, 1290
BanII GRGCYC 1 cut(s) 1005
BbvI GCAGC 4 cut(s) 113, 182, 188, 402
BccI CCATC 1 cut(s) 114
BceAI ACGGC 2 cut(s) 394, 1103
BcgI CGANNNNNNTGC 4 cut(s) 108, 142, 309, 343
BciT130I CCWGG 5 cut(s) 67, 333, 721, 987, 1114
BciVI GTATCC 1 cut(s) 73
BclI TGATCA 1 cut(s) 299
BcnI CCSGG 2 cut(s) 276, 890
BcoDI GTCTC 2 cut(s) 257, 496
BfaI CTAG 2 cut(s) 217, 238
BfmI CTRYAG 3 cut(s) 547, 994, 1205
BfrI CTTAAG 1 cut(s) 1245
BfuI GTATCC 1 cut(s) 73
BglII AGATCT 1 cut(s) 879
BisI GCNGC 4 cut(s) 127, 177, 196, 391
BlsI GCNGC 4 cut(s) 128, 178, 197, 392
Bme1390I CCNGG 7 cut(s) 67, 276, 333, 721, 890, 987, 1114
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 3 cut(s) 136, 398, 1116
BmiI GGNNCC 4 cut(s) 273, 705, 991, 1292
BmrFI CCNGG 7 cut(s) 67, 276, 333, 721, 890, 987, 1114
BmsI GCATC 1 cut(s) 1040
BmtI GCTAGC 1 cut(s) 220
Bpu10I CCTNAGC 1 cut(s) 394
BpuMI CCSGG 2 cut(s) 276, 890
BsaHI GRCGYC 1 cut(s) 893
BsaI GGTCTC 2 cut(s) 257, 496
BsaJI CCNNGG 7 cut(s) 66, 331, 376, 470, 719, 1113, 1306
BsaXI ACNNNNNCTCC 6 cut(s) 153, 183, 848, 878, 1120, 1150
Bse1I ACTGG 1 cut(s) 230
Bse21I CCTNAGG 1 cut(s) 541
Bse3DI GCAATG 1 cut(s) 102
BseBI CCWGG 5 cut(s) 67, 333, 721, 987, 1114
BseDI CCNNGG 7 cut(s) 66, 331, 376, 470, 719, 1113, 1306
BseGI GGATG 1 cut(s) 35
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 4 cut(s) 408, 507, 532, 1018
BseNI ACTGG 1 cut(s) 230
BseXI GCAGC 4 cut(s) 113, 182, 188, 402
BseYI CCCAGC 1 cut(s) 241
BshFI GGCC 3 cut(s) 381, 399, 1118
BshNI GGYRCC 2 cut(s) 271, 1290
BsiSI CCGG 4 cut(s) 27, 199, 276, 889
BslFI GGGAC 3 cut(s) 66, 275, 707
BsmAI GTCTC 2 cut(s) 257, 496
BsmFI GGGAC 3 cut(s) 66, 275, 707
BsnI GGCC 3 cut(s) 381, 399, 1118
Bso31I GGTCTC 2 cut(s) 257, 496
Bsp1286I GDGCHC 1 cut(s) 1005
Bsp143I GATC 6 cut(s) 80, 299, 626, 879, 989, 1278
BspACI CCGC 1 cut(s) 999
BspANI GGCC 3 cut(s) 381, 399, 1118
BspCNI CTCAG 4 cut(s) 407, 506, 533, 1017
BspHI TCATGA 1 cut(s) 422
BspLI GGNNCC 4 cut(s) 273, 705, 991, 1292
BspOI GCTAGC 1 cut(s) 220
BspPI GGATC 4 cut(s) 75, 634, 984, 997
BspT107I GGYRCC 2 cut(s) 271, 1290
BspTI CTTAAG 1 cut(s) 1245
BspTNI GGTCTC 2 cut(s) 257, 496
BsrDI GCAATG 1 cut(s) 102
BsrI ACTGG 1 cut(s) 230
BssECI CCNNGG 7 cut(s) 66, 331, 376, 470, 719, 1113, 1306
BssMI GATC 6 cut(s) 80, 299, 626, 879, 989, 1278
BssNI GRCGYC 1 cut(s) 893
BssT1I CCWWGG 2 cut(s) 470, 1306
Bst2UI CCWGG 5 cut(s) 67, 333, 721, 987, 1114
Bst6I CTCTTC 1 cut(s) 977
BstACI GRCGYC 1 cut(s) 893
BstAFI CTTAAG 1 cut(s) 1245
BstAPI GCANNNNNTGC 1 cut(s) 360
BstC8I GCNNGC 2 cut(s) 218, 1001
BstDEI CTNAG 5 cut(s) 394, 493, 541, 908, 1004
BstDSI CCRYGG 1 cut(s) 376
BstF5I GGATG 1 cut(s) 35
BstKTI GATC 6 cut(s) 83, 302, 629, 882, 992, 1281
BstMAI GTCTC 2 cut(s) 257, 496
BstMBI GATC 6 cut(s) 80, 299, 626, 879, 989, 1278
BstMWI GCNNNNNNNGC 4 cut(s) 192, 360, 387, 396
BstNI CCWGG 5 cut(s) 67, 333, 721, 987, 1114
BstNSI RCATGY 1 cut(s) 906
BstSCI CCNGG 7 cut(s) 65, 274, 331, 719, 888, 985, 1112
BstSFI CTRYAG 3 cut(s) 547, 994, 1205
BstV1I GCAGC 4 cut(s) 113, 182, 188, 402
BstX2I RGATCY 2 cut(s) 879, 989
BstYI RGATCY 2 cut(s) 879, 989
Bsu36I CCTNAGG 1 cut(s) 541
BsuI GTATCC 1 cut(s) 73
BsuRI GGCC 3 cut(s) 381, 399, 1118
BtgI CCRYGG 1 cut(s) 376
BtsCI GGATG 1 cut(s) 35
Cac8I GCNNGC 2 cut(s) 218, 1001
CaiI CAGNNNCTG 1 cut(s) 1011
CciI TCATGA 1 cut(s) 422
Cfr13I GGNCC 3 cut(s) 136, 398, 1116
CseI GACGC 1 cut(s) 882
Csp6I GTAC 5 cut(s) 40, 272, 314, 1291, 1311
CviAII CATG 6 cut(s) 423, 678, 903, 1037, 1199, 1323
CviQI GTAC 5 cut(s) 40, 272, 314, 1291, 1311
DdeI CTNAG 5 cut(s) 394, 493, 541, 908, 1004
DpnI GATC 6 cut(s) 82, 301, 628, 881, 991, 1280
DpnII GATC 6 cut(s) 80, 299, 626, 879, 989, 1278
DraIII CACNNNGTG 1 cut(s) 459
EaeI YGGCCR 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 977
EarI CTCTTC 1 cut(s) 977
Eco130I CCWWGG 2 cut(s) 470, 1306
Eco24I GRGCYC 1 cut(s) 1005
Eco31I GGTCTC 2 cut(s) 257, 496
Eco47I GGWCC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 232
Eco81I CCTNAGG 1 cut(s) 541
EcoRII CCWGG 5 cut(s) 65, 331, 719, 985, 1112
EcoT14I CCWWGG 2 cut(s) 470, 1306
EcoT38I GRGCYC 1 cut(s) 1005
ErhI CCWWGG 2 cut(s) 470, 1306
FaeI CATG 6 cut(s) 426, 681, 906, 1040, 1202, 1326
FalI AAGNNNNNCTT 2 cut(s) 1163, 1195
FaqI GGGAC 3 cut(s) 66, 275, 707
FatI CATG 6 cut(s) 422, 677, 902, 1036, 1198, 1322
FauI CCCGC 1 cut(s) 992
FauNDI CATATG 2 cut(s) 283, 860
FbaI TGATCA 1 cut(s) 299
Fnu4HI GCNGC 4 cut(s) 127, 177, 196, 391
FokI GGATG 1 cut(s) 42
FriOI GRGCYC 1 cut(s) 1005
Fsp4HI GCNGC 4 cut(s) 127, 177, 196, 391
FspBI CTAG 2 cut(s) 217, 238
GluI GCNGC 4 cut(s) 127, 177, 196, 391
GsaI CCCAGC 1 cut(s) 245
HaeIII GGCC 3 cut(s) 381, 399, 1118
HapII CCGG 4 cut(s) 27, 199, 276, 889
HgaI GACGC 1 cut(s) 882
Hin1I GRCGYC 1 cut(s) 893
Hin1II CATG 6 cut(s) 426, 681, 906, 1040, 1202, 1326
HincII GTYRAC 2 cut(s) 620, 1260
HindII GTYRAC 2 cut(s) 620, 1260
HinfI GANTC 2 cut(s) 561, 1188
HpaI GTTAAC 1 cut(s) 620
HpaII CCGG 4 cut(s) 27, 199, 276, 889
HphI GGTGA 2 cut(s) 294, 693
Hpy166II GTNNAC 5 cut(s) 35, 110, 620, 856, 1260
Hpy188I TCNGA 5 cut(s) 642, 788, 808, 1018, 1278
Hpy188III TCNNGA 4 cut(s) 297, 423, 477, 839
Hpy8I GTNNAC 5 cut(s) 35, 110, 620, 856, 1260
HpyAV CCTTC 1 cut(s) 941
HpyCH4V TGCA 4 cut(s) 176, 371, 759, 1040
HpyF10VI GCNNNNNNNGC 4 cut(s) 192, 360, 387, 396
HpyF3I CTNAG 5 cut(s) 394, 493, 541, 908, 1004
Hsp92I GRCGYC 1 cut(s) 893
Hsp92II CATG 6 cut(s) 426, 681, 906, 1040, 1202, 1326
KpnI GGTACC 2 cut(s) 275, 1294
Ksp22I TGATCA 1 cut(s) 299
KspAI GTTAAC 1 cut(s) 620
Kzo9I GATC 6 cut(s) 80, 299, 626, 879, 989, 1278
LmnI GCTCC 3 cut(s) 123, 642, 869
Lsp1109I GCAGC 4 cut(s) 113, 182, 188, 402
LweI GCATC 1 cut(s) 1040
MaeI CTAG 2 cut(s) 217, 238
MaeIII GTNAC 6 cut(s) 143, 206, 451, 465, 911, 1228
MalI GATC 6 cut(s) 82, 301, 628, 881, 991, 1280
MboI GATC 6 cut(s) 80, 299, 626, 879, 989, 1278
MboII GAAGA 4 cut(s) 21, 874, 889, 964
MfeI CAATTG 1 cut(s) 325
MflI RGATCY 2 cut(s) 879, 989
MhlI GDGCHC 1 cut(s) 1005
MluCI AATT 7 cut(s) 131, 325, 443, 506, 599, 780, 818
MlyI GAGTC 1 cut(s) 570
MmeI TCCRAC 4 cut(s) 854, 891, 1106, 1118
MnlI CCTC 8 cut(s) 98, 473, 536, 677, 782, 907, 1122, 1219
MroXI GAANNNNTTC 1 cut(s) 881
MseI TTAA 4 cut(s) 619, 733, 745, 1246
MslI CAYNNNNRTG 3 cut(s) 427, 942, 1321
MspCI CTTAAG 1 cut(s) 1245
MspI CCGG 4 cut(s) 27, 199, 276, 889
MspR9I CCNGG 7 cut(s) 67, 276, 333, 721, 890, 987, 1114
MunI CAATTG 1 cut(s) 325
MvaI CCWGG 5 cut(s) 67, 333, 721, 987, 1114
MwoI GCNNNNNNNGC 4 cut(s) 192, 360, 387, 396
NciI CCSGG 2 cut(s) 276, 890
NdeI CATATG 2 cut(s) 283, 860
NdeII GATC 6 cut(s) 80, 299, 626, 879, 989, 1278
NheI GCTAGC 1 cut(s) 216
NlaIII CATG 6 cut(s) 426, 681, 906, 1040, 1202, 1326
NlaIV GGNNCC 4 cut(s) 273, 705, 991, 1292
NmuCI GTSAC 2 cut(s) 206, 451
NspI RCATGY 1 cut(s) 906
PagI TCATGA 1 cut(s) 422
PdmI GAANNNNTTC 1 cut(s) 881
PfeI GAWTC 1 cut(s) 1188
PflMI CCANNNNNTGG 3 cut(s) 576, 593, 873
PkrI GCNGC 4 cut(s) 128, 178, 197, 392
PleI GAGTC 1 cut(s) 569
PpsI GAGTC 1 cut(s) 569
PsiI TTATAA 1 cut(s) 502
Psp6I CCWGG 5 cut(s) 65, 331, 719, 985, 1112
PspFI CCCAGC 1 cut(s) 241
PspGI CCWGG 5 cut(s) 65, 331, 719, 985, 1112
PspN4I GGNNCC 4 cut(s) 273, 705, 991, 1292
PspPI GGNCC 3 cut(s) 136, 398, 1116
PstNI CAGNNNCTG 1 cut(s) 1011
PsuI RGATCY 2 cut(s) 879, 989
RsaI GTAC 5 cut(s) 41, 273, 315, 1292, 1312
RsaNI GTAC 5 cut(s) 40, 272, 314, 1291, 1311
RseI CAYNNNNRTG 3 cut(s) 427, 942, 1321
SaqAI TTAA 4 cut(s) 619, 733, 745, 1246
SatI GCNGC 4 cut(s) 127, 177, 196, 391
Sau3AI GATC 6 cut(s) 80, 299, 626, 879, 989, 1278
Sau96I GGNCC 3 cut(s) 136, 398, 1116
SchI GAGTC 1 cut(s) 570
ScrFI CCNGG 7 cut(s) 67, 276, 333, 721, 890, 987, 1114
SduI GDGCHC 1 cut(s) 1005
SfaNI GCATC 1 cut(s) 1040
SfcI CTRYAG 3 cut(s) 547, 994, 1205
SinI GGWCC 1 cut(s) 136
SmiMI CAYNNNNRTG 3 cut(s) 427, 942, 1321
SmlI CTYRAG 1 cut(s) 1245
SmoI CTYRAG 1 cut(s) 1245
Sse9I AATT 7 cut(s) 131, 325, 443, 506, 599, 780, 818
SsiI CCGC 1 cut(s) 999
SspMI CTAG 2 cut(s) 217, 238
StyD4I CCNGG 7 cut(s) 65, 274, 331, 719, 888, 985, 1112
StyI CCWWGG 2 cut(s) 470, 1306
TaqI TCGA 1 cut(s) 118
TasI AATT 7 cut(s) 131, 325, 443, 506, 599, 780, 818
TfiI GAWTC 1 cut(s) 1188
Tru1I TTAA 4 cut(s) 619, 733, 745, 1246
Tru9I TTAA 4 cut(s) 619, 733, 745, 1246
TseFI GTSAC 2 cut(s) 206, 451
TseI GCWGC 4 cut(s) 126, 176, 195, 390
Tsp45I GTSAC 2 cut(s) 206, 451
TspDTI ATGAA 7 cut(s) 334, 592, 784, 960, 1025, 1183, 1311
Van91I CCANNNNNTGG 3 cut(s) 576, 593, 873
Vha464I CTTAAG 1 cut(s) 1245
VpaK11BI GGWCC 1 cut(s) 136
XceI RCATGY 1 cut(s) 906
XmnI GAANNNNTTC 1 cut(s) 881
XspI CTAG 2 cut(s) 217, 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.