Rroxscaffold_4G00306240

Protein transport protein sec16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001182
Physical Location & Seq
Reverse (-)
27027546 .. 27035672
8127 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306240.1

Sequence Viewer

Length: 4911 bp
ATGGCAAGTTCTGCGTTTGAGTTGGAGGATCAGACGGATGAGGATTTCTTTGACAAATTGATCGATGATGATGACATTGATTTTTCTGGGTATGCACATAGTTCTGTAGCAAAGGTTGAGCTTGATGTGGTTAATGATATTTCGAAATTGAGTATTTCGGATGTAGGTACCGGTGGGAAAACTAGTTCTCAGGTTAATGCTGAGTTGGGTCAGAAAGACGGGGTTGTGTTGCAGCCGTCGGATCTGCAGAAGGAGTCTTTGACTGGTAGAGAGAGTGAGTCTAGTGCATCGGTGGCCAAAGGGGAGAAAGGGGTGGTGGATGATACAGTGAGTACGAGTGTTGTATCAGTTGATGATGGATTGGGTCGCGGGGATGGGGTTGTGTTAGCTTCGTTGGATCGACACCAGGATCCTATAGTCGGTGAAGTGAGCAAGTCATCAGTACCGGAAAATAGAGATGAGGCAGTGGAGGATAAAGCTAGTAATGAGTGTGGTGAATTGGAGGGTAAAGGGGAGGACAGTTCTGTAGATAATACAGTCGGAAAGAGTGTGGTGTCAGAGGGAACAGGTGTTAAAGTAGTTCAATGGAGTTCGTTTAACTCAGATCCTTCTGGTGGTAGTGGATTTGGTGAAGTTGCAGATAACTCGGAAGACCCATTTGCGAATTTGGGTAACACAGACCAATCTGGGGTCAAATCTATTGTGTCTAATAGTGTTCTGGAAAGCTCAGTTTCTGCTTTCGGTAATTCTAGCTATGTGCAGCATCAAGAAGACCCATTTGCAAACTTGGGTAATACAGTCCAATTTGGGGCTGAATCCGTTGTGACTAATGGTGTTCTGGAAAATGCTGTTTCTGGTTTTGATACTTTTAGCTATGGGCAGCAACAAGAAGACCCATTTGCAGATTTGGGTAATGCAGACCAATTTGGGGCCAAATCTGCTGTGAGTAATGTTGTTCTGGAAAACTCGGGTTCTGTTTTGGGTGCTTCTATCTATGGGAAGCATCAAGAAGATCCATTTGCGAATTTGGGTAATACAGACCAGTCTGGGGCTGAATCTAATGTGAGTAATGGTGTTTTGGAGAACTCAGTTTCTGGTTTGGGTACTTCTAGCTATGGGCAGCATCAAGAAGGTCAATTCTATGGGGCTCCTGAAAATCAAAACTTAGACGGGCAAGAATTAAATAGTAATCAGAATTGGGAAAATCTTTATCCCGGATGGAGGTACGATCCAAATACTGAGCAGTGGTATCCGTTGGAGGGTTACGATGTGAATTCAAACACAAATGCAGAGGTGAACAATAATGCAATTGCTCAGGAGAGTTTTAATAAAGCAGATGCTCCTTATCTGCAGCAAGCAGTGCAGGCTGTTTCTCAAAGTGTGGTTGAGGCATCTGAAAGTAGCACAGTTTCTAACTGGAACCAGCATTCTTATGGGAACACAGAATACCCTGCACATATGATTTTTGATCCCCAGTACCCCGGTTGGTACTATGACACAAATATCCAAGAATGGAGGCAGTTGGAACCTTCTGTAGCTACAATCTACTATGATCAGCAAAATACGAGTATGTGGCATGCTGAGAATGTTGCCAAGAGTGAAACTATTGGTTTTACTGAGAAACAGGAATGGAGACAGGTGCAGCCTTTCACTCCATCTTTTGATCAGTCAACAACAAGTACGGTTCATAATCAGCAATATAACAATACAAATGTTGACAACCATGGATCACAGAGTCCAATCATTCAACAAAGTGTTTTAAACTGGGATGGATCTATGACCACCTATGATCAGCAAAATACGAGTTTTGGGCATGCTCAGAAAGTTGCCAAGAGTGATACTGTTGGTTTTACTGAGAAACAGCCATACATGGACCGGCAATTTTCAACTGAGCATGCATTTAATTCTGTGAATCAACAAACAGGGTTCAATCCTTCAGGATCAATTGCTCTTCATCAAGACCCAAGTCAGGGCAATGATATTAGTAGTGCGATTGGTGGCTTTGAAAGGTTTAATTATGCTAACTTTTCTCAGCCTCAGGATCAAGCCAAGGAGCCAAACCAAGTGATGCATTTCTCACCTGCTAACTTTGGTAGTCAGAAGCCAGTACAATTTTTCCAGCAGCCACTCCAGACTGGAACTCAGTTTTCACACGAGGCCAGAGAGGGGAGGTCATCTGCTGGACGACCTGCACATGCTCTGGTTACTTTTGGATTTGGTGGGAAACTTATAGTAATGAAAAATAATAGTTCTTTTCATGCAAACCAGACATATGGAAGCCAGGGCTCTGTAGAAGGTGTCGTCAATGTTTACAATCTGATGGATGTTGTCACGGAGAAAAGTGATGCTGCAAACTTTGGAACTGATGGTTGTGATTATTTCAGCGCCTTGTTCCAGCAATCTTTTCCTGGACCTTTAGTTGGTGGGAATGTCGGAAATAAGGAATTAAACAAATGGATTGATGACAAAATTGCAAATTGTGAAACTTCTCACTTGGATTTCAAGAAAGGGGACCATTTGAGATTGCTTTTCTCTTTACTAAAAATATCTTGGCAATTTTATGGAAAACTGCGATCTCCCTTTGGTACCGATCAAAGATTGAAGGACATTGATAATCCAGAATCAGCTCTTGCTAAACTTTTTGCATCTGCTAAAAGAAGTAACGAGTATGGTGCTCTTACGCGGTGCATGCATAACTTGCCTTCTGAAGCACAAATTCAGGCTACTGCTCTTGAGGTACAAAAGCTTCTCGTCTCTGGTAGGAAGGATGAGGCTTTACAATGCGCACAAGGAGGTCAATTGTGGGGACCAGCACTTGTTATTGCATCGCAACTTGGGGATCAGTTTTATTGTGATACTGTGAAGCATATGGCTGTAAACCAGTTAATAGCAGGATCACCATTGCGGACGTTGTGCTTGTTAATTGCCAGGCAGCCAGCAGATGTGTTTTCCGATGCTACTACAAATAGCAGTTTTCCTAGTGTCACAAATTTATCACAACGGCACGAACAGGTTGGAGCTAATTACATGTTAGATGACTGGGAAGAGAATCTAGCCATCTTAACTGCAAATAGAACAAAAGATGATGAGCTTGTTATCATCCATCTTGGAGATTCCTTATGGAAGGATAGGGGACAGATAACTGCTGCACACATATGTTATTTAGTTGCAGAAGCCAACCTTGAGCAATATTCGAACAGTTCAAGACTGTGTCTTATCGGTGCTGATCATTGGAAGTTTCCACGTACATACGCCAGTCCAGAGGCTATTCAGAGGACAGAGCTGTATGAATACTCAAAGGTTGTTGGAAATCCTCAGTTTCTTCTACTACCATTTCAGCCATATAAGCTTATTTATGCCCACATGCTGGCTGAAGTAGGAAAACTTGCTGATGCTTTGAAATATTGTCAAGCAATTTTGAAGTCTTTGAAAACTGCTCGAGCACCTGAGGTGGACACATGGAGAGAGTTGGTGTCATCTCTGGAGGAGCGGATAAGAGCCCACCAGCAGGGTGGTTATAGCATAAATTTGGCTCCAACCAAGTTGATGGGTAAATTGCGTACATTCATTGATAGTACTGCTCATCGTGTTGTTGGGGGGTTACCTCCACCTATACCATCAACAGCACCAGGCTTTCCTCAGCTTAATGAACATGCTCATCAACTACCTCCACCTATACCATCAACAGCACCAGGCTTCCCTCAGCTTAATGAACATGCTCATCAACTACCTCCACCTATACCATCAACAGCACTGGGCTTTGCTCAGCTTAATGAACATGCTCATCAACCAGGAAATCCAAATGTACCCAACAGTCAATCGACAATGGTTATGTCTTCAATAATGCCTTCAGCATCAATGGAGCCAATAAGTGAAAGGACTTCTGAGAGCAACCAATTGAATATGCCTAACAGAAGCATTTCAGAGCCAGACTTTGGGAAAAGCCCGAGTAAGGTCGATTCATCCAAGAAAGTGAACTCCTCTAAAACGCAACAAAAGCCATCAGCATCTAGTGGGTCACGTTTCAGTTTTGGGTCACAGATATTTCAAAAGACTCTAGGACTGGTCATAAAATCCCGGCCAGATAAGCAGGCTAAATTGGGTGAAGCAAATAAATTCTACTATGATGAGAAACTCAAGCGATGGGTCGAAGAAGGTGCTGAACCTCCAGCAGAGGAAGCAGCCTTACCACCACCTCCGCCAACAACTGCAGCTATCCAGAATAGAATGCAAGATTACAACATGAATGATGCACTGAAAACTACTAGTTTTCATGCAGTTGGTGGGCCAGAAATCAAAAGCCCAGCATCTTCCGAGAATTCAGGGGCACCACCCGTTCCACCCAGTTCAAACCAGTTCTCTGCACGTGGGCGTATGGGTGTTCGCTCAAGGTATGTTGATACATTCAACAAAGGAGGTGCGACCCCAGGAAACTTATTTCAGCCACCATCTCTTCCAGCTGCTAAACCAGGGGGTGGTTCCAATCCCAAGTTTTTCATCCCCTCTCCAGTAACTTCCTATCAGGAAACAGTTCAAACAACTGAAGGAAGCACACAAGAATCTTTTGTGACCAGTAACAATCCTCCAAAATCAGTAGACACATTCTCTACTCCGCCAACCTCAACACCATCGTCAATGACCATGCAACGGTTTCCTAGCATGGATAACATTGTACAAAAGAGAGCAGGAGAATTGGCAAATGGCAGTAGCTTTGTTCCACCTGAATCTCGTCGTGTGGCATCATGGAGTGGAAGCCTTAGTGAAGTAAGCAATCCTTCGGTAAGGAGTGAAATAAAACCAACAGCACTTGGTATGTCCCCATTGTCATATATGCCTAGTTTTTCTCCATCAATGCAGTCTGAAAGAAGCAGTGGTAGTTTGGGGGATGACCTTCATGAAGTTGAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec16 Sec16_C

Protein Analysis

1636

Amino Acids

177.77

Weight (kDa)

4.91

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec16 PF12932 732 - 855 6.3e-21 Vesicle coat trafficking protein Sec16 mid-region
TPR_Sec16 PF12931 913 - 1200 1.1e-54 Sec16 Sec23-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2089
Acc16I TGCGCA 1 cut(s) 2785
Acc36I ACCTGC 2 cut(s) 2089, 2197
Acc65I GGTACC 2 cut(s) 167, 2585
AccB1I GGYRCC 3 cut(s) 167, 2585, 4327
AccB7I CCANNNNNTGG 3 cut(s) 3367, 3935, 4475
AccBSI CCGCTC 1 cut(s) 3490
AccI GTMKAC 1 cut(s) 4596
AccII CGCG 2 cut(s) 369, 2683
AciI CCGC 6 cut(s) 369, 2683, 2905, 3490, 4199, 4613
AcoI YGGCCR 2 cut(s) 294, 4079
AcsI RAATTY 8 cut(s) 664, 1024, 1273, 2715, 2989, 3526, 4115, 4318
AcuI CTGAAG 5 cut(s) 1920, 2727, 3393, 3834, 4563
AcvI CACGTG 1 cut(s) 4367
AflIII ACRYGT 1 cut(s) 3027
AgeI ACCGGT 1 cut(s) 170
AhlI ACTAGT 2 cut(s) 182, 4265
AjnI CCWGG 9 cut(s) 405, 2280, 2407, 2927, 3628, 3691, 3790, 4426, 4468
AloI GAACNNNNNNTCC 2 cut(s) 169, 201
Alw21I GWGCWC 2 cut(s) 2677, 3445
Alw26I GTCTC 2 cut(s) 1627, 2758
AlwNI CAGNNNCTG 2 cut(s) 734, 1094
Ama87I CYCGRG 3 cut(s) 967, 3438, 3946
AoxI GGCC 5 cut(s) 294, 930, 2157, 4079, 4286
ApoI RAATTY 8 cut(s) 664, 1024, 1273, 2715, 2989, 3526, 4115, 4318
AsiGI ACCGGT 1 cut(s) 170
Asp700I GAANNNNTTC 2 cut(s) 3919, 4530
Asp718I GGTACC 2 cut(s) 167, 2585
AspLEI GCGC 2 cut(s) 2387, 2786
AspS9I GGNCC 6 cut(s) 930, 1873, 2411, 2512, 2807, 4286
AsuC2I CCSGG 3 cut(s) 1215, 1482, 4078
AsuHPI GGTGA 7 cut(s) 434, 506, 641, 1306, 2070, 2889, 4115
AsuII TTCGAA 2 cut(s) 143, 3194
AvaI CYCGRG 3 cut(s) 967, 3438, 3946
AvaII GGWCC 4 cut(s) 1873, 2411, 2512, 2807
AxyI CCTNAGG 2 cut(s) 2037, 3447
BaeGI GKGCMC 1 cut(s) 4330
BalI TGGCCA 1 cut(s) 296
BamHI GGATCC 1 cut(s) 409
BanI GGYRCC 3 cut(s) 167, 2585, 4327
BanII GRGCYC 3 cut(s) 1150, 2288, 3502
BarI GAAGNNNNNNTAC 4 cut(s) 2730, 2762, 4170, 4202
BauI CACGAG 1 cut(s) 2153
BbrPI CACGTG 1 cut(s) 4367
BbsI GAAGAC 4 cut(s) 657, 777, 897, 3828
Bbv12I GWGCWC 2 cut(s) 2677, 3445
BbvCI CCTCAGC 2 cut(s) 3639, 3702
BceAI ACGGC 2 cut(s) 220, 3017
BcgI CGANNNNNNTGC 6 cut(s) 627, 661, 2654, 2688, 4139, 4173
BciT130I CCWGG 9 cut(s) 407, 2282, 2409, 2929, 3630, 3693, 3792, 4428, 4470
BciVI GTATCC 1 cut(s) 1260
BclI TGATCA 4 cut(s) 1552, 1663, 1789, 3226
BcnI CCSGG 3 cut(s) 1215, 1482, 4078
BcoDI GTCTC 2 cut(s) 1627, 2758
BcuI ACTAGT 2 cut(s) 182, 4265
BfmI CTRYAG 8 cut(s) 105, 245, 414, 525, 1349, 1533, 2289, 4209
BfoI RGCGCY 1 cut(s) 2388
BfuAI ACCTGC 2 cut(s) 2089, 2197
BfuI GTATCC 1 cut(s) 1260
BlpI GCTNAGC 1 cut(s) 3765
BmcAI AGTACT 1 cut(s) 3577
Bme18I GGWCC 4 cut(s) 1873, 2411, 2512, 2807
BmeT110I CYCGRG 3 cut(s) 967, 3438, 3946
BmgT120I GGNCC 6 cut(s) 930, 1873, 2411, 2512, 2807, 4286
BmrI ACTGGG 5 cut(s) 1468, 1774, 3049, 3764, 4338
BmuI ACTGGG 5 cut(s) 1468, 1774, 3049, 3764, 4338
BoxI GACNNNNGTC 1 cut(s) 1965
BpiI GAAGAC 4 cut(s) 657, 777, 897, 3828
BpmI CTGGAG 4 cut(s) 2114, 3503, 4152, 4491
Bpu10I CCTNAGC 3 cut(s) 1314, 3639, 3702
Bpu1102I GCTNAGC 1 cut(s) 3765
Bpu14I TTCGAA 2 cut(s) 143, 3194
BpuEI CTTGAG 4 cut(s) 2753, 3203, 4121, 4372
BpuMI CCSGG 3 cut(s) 1215, 1482, 4078
Bsa29I ATCGAT 1 cut(s) 63
BsaAI YACGTR 2 cut(s) 3245, 4367
BsaJI CCNNGG 6 cut(s) 1480, 1723, 2049, 2281, 4426, 4469
BsaWI WCCGGW 2 cut(s) 170, 445
Bse118I RCCGGY 2 cut(s) 170, 1875
Bse21I CCTNAGG 2 cut(s) 2037, 3447
Bse3DI GCAATG 2 cut(s) 1981, 2900
BseBI CCWGG 9 cut(s) 407, 2282, 2409, 2929, 3630, 3693, 3792, 4428, 4470
BseCI ATCGAT 1 cut(s) 63
BseDI CCNNGG 6 cut(s) 1480, 1723, 2049, 2281, 4426, 4469
BseMI GCAATG 2 cut(s) 1981, 2900
BseRI GAGGAG 2 cut(s) 3500, 3970
BseSI GKGCMC 1 cut(s) 4330
BseYI CCCAGC 1 cut(s) 4303
BsgI GTGCAG 7 cut(s) 779, 1382, 1437, 1661, 2175, 3132, 4347
Bsh1236I CGCG 2 cut(s) 369, 2683
BshFI GGCC 5 cut(s) 296, 932, 2159, 4081, 4288
BshNI GGYRCC 3 cut(s) 167, 2585, 4327
BshTI ACCGGT 1 cut(s) 170
BshVI ATCGAT 1 cut(s) 63
BsiHKAI GWGCWC 2 cut(s) 2677, 3445
BsiHKCI CYCGRG 3 cut(s) 967, 3438, 3946
BsiSI CCGG 6 cut(s) 171, 446, 1215, 1482, 1876, 4078
BslFI GGGAC 4 cut(s) 2525, 2820, 3147, 4801
BsmAI GTCTC 2 cut(s) 1627, 2758
BsmBI CGTCTC 1 cut(s) 2758
BsmFI GGGAC 4 cut(s) 2525, 2820, 3147, 4801
BsmI GAATGC 2 cut(s) 1426, 4233
BsnI GGCC 5 cut(s) 296, 932, 2159, 4081, 4288
BsoBI CYCGRG 3 cut(s) 967, 3438, 3946
Bsp119I TTCGAA 2 cut(s) 143, 3194
Bsp1286I GDGCHC 6 cut(s) 1150, 2288, 2677, 3445, 3502, 4330
Bsp1407I TGTACA 1 cut(s) 4672
Bsp1720I GCTNAGC 1 cut(s) 3765
Bsp19I CCATGG 1 cut(s) 1723
BspACI CCGC 6 cut(s) 369, 2683, 2905, 3490, 4199, 4613
BspANI GGCC 5 cut(s) 296, 932, 2159, 4081, 4288
BspDI ATCGAT 1 cut(s) 63
BspFNI CGCG 2 cut(s) 369, 2683
BspHI TCATGA 1 cut(s) 4894
BspMAI CTGCAG 3 cut(s) 249, 1353, 4213
BspMI ACCTGC 2 cut(s) 2089, 2197
BspQI GCTCTTC 1 cut(s) 1956
BspT104I TTCGAA 2 cut(s) 143, 3194
BspT107I GGYRCC 3 cut(s) 167, 2585, 4327
BsrBI CCGCTC 1 cut(s) 3490
BsrDI GCAATG 2 cut(s) 1981, 2900
BsrFI RCCGGY 2 cut(s) 170, 1875
BsrGI TGTACA 1 cut(s) 4672
BssAI RCCGGY 2 cut(s) 170, 1875
BssECI CCNNGG 6 cut(s) 1480, 1723, 2049, 2281, 4426, 4469
BssSI CACGAG 1 cut(s) 2153
BssT1I CCWWGG 2 cut(s) 1723, 2049
Bst2BI CACGAG 1 cut(s) 2153
Bst2UI CCWGG 9 cut(s) 407, 2282, 2409, 2929, 3630, 3693, 3792, 4428, 4470
Bst6I CTCTTC 3 cut(s) 1956, 3039, 4458
BstAPI GCANNNNNTGC 3 cut(s) 11, 1360, 2698
BstAUI TGTACA 1 cut(s) 4672
BstBAI YACGTR 2 cut(s) 3245, 4367
BstBI TTCGAA 2 cut(s) 143, 3194
BstC8I GCNNGC 9 cut(s) 1356, 1365, 1578, 1815, 1896, 2690, 2937, 3369, 4092
BstDSI CCRYGG 1 cut(s) 1723
BstEII GGTNACC 1 cut(s) 3600
BstFNI CGCG 2 cut(s) 369, 2683
BstH2I RGCGCY 1 cut(s) 2388
BstHHI GCGC 2 cut(s) 2387, 2786
BstMAI GTCTC 2 cut(s) 1627, 2758
BstNI CCWGG 9 cut(s) 407, 2282, 2409, 2929, 3630, 3693, 3792, 4428, 4470
BstPAI GACNNNNGTC 1 cut(s) 1965
BstPI GGTNACC 1 cut(s) 3600
BstSFI CTRYAG 8 cut(s) 105, 245, 414, 525, 1349, 1533, 2289, 4209
BstSLI GKGCMC 1 cut(s) 4330
BstUI CGCG 2 cut(s) 369, 2683
BstV2I GAAGAC 4 cut(s) 657, 777, 897, 3828
BstX2I RGATCY 5 cut(s) 241, 409, 604, 1012, 1772
BstXI CCANNNNNNTGG 3 cut(s) 2273, 3512, 3547
BstYI RGATCY 5 cut(s) 241, 409, 604, 1012, 1772
Bsu15I ATCGAT 1 cut(s) 63
Bsu36I CCTNAGG 2 cut(s) 2037, 3447
BsuI GTATCC 1 cut(s) 1260
BsuRI GGCC 5 cut(s) 296, 932, 2159, 4081, 4288
BsuTUI ATCGAT 1 cut(s) 63
BtgI CCRYGG 1 cut(s) 1723
BtgZI GCGATG 2 cut(s) 2811, 4156
BtsI GCAGTG 4 cut(s) 471, 1250, 1365, 4876
BtsIMutI CAGTG 7 cut(s) 333, 471, 1250, 1365, 3752, 4253, 4876
BveI ACCTGC 2 cut(s) 2089, 2197
Cac8I GCNNGC 9 cut(s) 1356, 1365, 1578, 1815, 1896, 2690, 2937, 3369, 4092
CaiI CAGNNNCTG 2 cut(s) 734, 1094
CciI TCATGA 1 cut(s) 4894
CfoI GCGC 2 cut(s) 2387, 2786
Cfr10I RCCGGY 2 cut(s) 170, 1875
Cfr13I GGNCC 6 cut(s) 930, 1873, 2411, 2512, 2807, 4286
ClaI ATCGAT 1 cut(s) 63
CspAI ACCGGT 1 cut(s) 170
DraI TTTAAA 1 cut(s) 1761
EaeI YGGCCR 2 cut(s) 294, 4079
Eam1104I CTCTTC 3 cut(s) 1956, 3039, 4458
EarI CTCTTC 3 cut(s) 1956, 3039, 4458
EciI GGCGGA 2 cut(s) 4188, 4602
Eco130I CCWWGG 2 cut(s) 1723, 2049
Eco24I GRGCYC 3 cut(s) 1150, 2288, 3502
Eco47I GGWCC 4 cut(s) 1873, 2411, 2512, 2807
Eco57I CTGAAG 5 cut(s) 1920, 2727, 3393, 3834, 4563
Eco72I CACGTG 1 cut(s) 4367
Eco81I CCTNAGG 2 cut(s) 2037, 3447
Eco88I CYCGRG 3 cut(s) 967, 3438, 3946
Eco91I GGTNACC 1 cut(s) 3600
EcoO65I GGTNACC 1 cut(s) 3600
EcoRI GAATTC 2 cut(s) 1273, 4318
EcoRII CCWGG 9 cut(s) 405, 2280, 2407, 2927, 3628, 3691, 3790, 4426, 4468
EcoT14I CCWWGG 2 cut(s) 1723, 2049
EcoT22I ATGCAT 3 cut(s) 1900, 2073, 2694
EcoT38I GRGCYC 3 cut(s) 1150, 2288, 3502
ErhI CCWWGG 2 cut(s) 1723, 2049
Esp3I CGTCTC 1 cut(s) 2758
FalI AAGNNNNNCTT 4 cut(s) 3165, 3197, 4759, 4791
FaqI GGGAC 4 cut(s) 2525, 2820, 3147, 4801
FauI CCCGC 1 cut(s) 362
FauNDI CATATG 4 cut(s) 1458, 2272, 2868, 3155
FbaI TGATCA 4 cut(s) 1552, 1663, 1789, 3226
FblI GTMKAC 1 cut(s) 4596
FriOI GRGCYC 3 cut(s) 1150, 2288, 3502
FspAI RTGCGCAY 1 cut(s) 2785
FspI TGCGCA 1 cut(s) 2785
GlaI GCGC 2 cut(s) 2386, 2785
GsaI CCCAGC 1 cut(s) 4307
GsuI CTGGAG 4 cut(s) 2114, 3503, 4152, 4491
HaeII RGCGCY 1 cut(s) 2388
HaeIII GGCC 5 cut(s) 296, 932, 2159, 4081, 4288
HapII CCGG 6 cut(s) 171, 446, 1215, 1482, 1876, 4078
HhaI GCGC 2 cut(s) 2387, 2786
Hin6I GCGC 2 cut(s) 2385, 2784
HinP1I GCGC 2 cut(s) 2385, 2784
HincII GTYRAC 2 cut(s) 1671, 1717
HindII GTYRAC 2 cut(s) 1671, 1717
HindIII AAGCTT 2 cut(s) 2744, 3347
HpaII CCGG 6 cut(s) 171, 446, 1215, 1482, 1876, 4078
HphI GGTGA 7 cut(s) 434, 506, 641, 1306, 2070, 2889, 4115
Hpy166II GTNNAC 8 cut(s) 1297, 1671, 1717, 2311, 2878, 3454, 3976, 4597
Hpy8I GTNNAC 8 cut(s) 1297, 1671, 1717, 2311, 2878, 3454, 3976, 4597
Hpy99I CGWCG 2 cut(s) 241, 4734
HpyCH4IV ACGT 4 cut(s) 2909, 3244, 4021, 4366
HpySE526I ACGT 4 cut(s) 2909, 3244, 4021, 4366
HspAI GCGC 2 cut(s) 2385, 2784
KpnI GGTACC 2 cut(s) 171, 2589
Ksp22I TGATCA 4 cut(s) 1552, 1663, 1789, 3226
LguI GCTCTTC 1 cut(s) 1956
LmnI GCTCC 7 cut(s) 1153, 1345, 2053, 3017, 3487, 3538, 3862
MaeII ACGT 4 cut(s) 2909, 3244, 4021, 4366
MbiI CCGCTC 1 cut(s) 3490
MfeI CAATTG 4 cut(s) 1308, 1944, 2798, 3896
MflI RGATCY 5 cut(s) 241, 409, 604, 1012, 1772
MhlI GDGCHC 6 cut(s) 1150, 2288, 2677, 3445, 3502, 4330
MlsI TGGCCA 1 cut(s) 296
MluNI TGGCCA 1 cut(s) 296
MlyI GAGTC 4 cut(s) 263, 287, 1744, 4048
MmeI TCCRAC 9 cut(s) 219, 375, 520, 1236, 1503, 2413, 2995, 3286, 3560
Mox20I TGGCCA 1 cut(s) 296
Mph1103I ATGCAT 3 cut(s) 1900, 2073, 2694
MroXI GAANNNNTTC 2 cut(s) 3919, 4530
MscI TGGCCA 1 cut(s) 296
MslI CAYNNNNRTG 2 cut(s) 1431, 3154
Msp20I TGGCCA 1 cut(s) 296
MspA1I CMGCKG 1 cut(s) 4460
MspI CCGG 6 cut(s) 171, 446, 1215, 1482, 1876, 4078
MunI CAATTG 4 cut(s) 1308, 1944, 2798, 3896
Mva1269I GAATGC 2 cut(s) 1426, 4233
MvaI CCWGG 9 cut(s) 407, 2282, 2409, 2929, 3630, 3693, 3792, 4428, 4470
MvnI CGCG 2 cut(s) 369, 2683
NciI CCSGG 3 cut(s) 1215, 1482, 4078
NcoI CCATGG 1 cut(s) 1723
NdeI CATATG 4 cut(s) 1458, 2272, 2868, 3155
NmuCI GTSAC 6 cut(s) 823, 2329, 2983, 4017, 4035, 4567
NsbI TGCGCA 1 cut(s) 2785
NsiI ATGCAT 3 cut(s) 1900, 2073, 2694
NspV TTCGAA 2 cut(s) 143, 3194
PaeI GCATGC 4 cut(s) 1580, 1817, 1898, 2692
PaeR7I CTCGAG 1 cut(s) 3438
PagI TCATGA 1 cut(s) 4894
PaqCI CACCTGC 1 cut(s) 2089
PciI ACATGT 1 cut(s) 3027
PciSI GCTCTTC 1 cut(s) 1956
PctI GAATGC 2 cut(s) 1426, 4233
PdmI GAANNNNTTC 2 cut(s) 3919, 4530
PfeI GAWTC 9 cut(s) 815, 1055, 1912, 2621, 3049, 3113, 3959, 4559, 4724
PflFI GACNNNGTC 1 cut(s) 3210
PflMI CCANNNNNTGG 3 cut(s) 3367, 3935, 4475
PfoI TCCNGGA 2 cut(s) 1213, 2407
PinAI ACCGGT 1 cut(s) 170
PleI GAGTC 4 cut(s) 262, 286, 1743, 4048
PmaCI CACGTG 1 cut(s) 4367
PmlI CACGTG 1 cut(s) 4367
PpsI GAGTC 4 cut(s) 262, 286, 1743, 4048
Ppu21I YACGTR 2 cut(s) 3245, 4367
PscI ACATGT 1 cut(s) 3027
PshAI GACNNNNGTC 1 cut(s) 1965
Psp6I CCWGG 9 cut(s) 405, 2280, 2407, 2927, 3628, 3691, 3790, 4426, 4468
PspCI CACGTG 1 cut(s) 4367
PspEI GGTNACC 1 cut(s) 3600
PspFI CCCAGC 1 cut(s) 4303
PspGI CCWGG 9 cut(s) 405, 2280, 2407, 2927, 3628, 3691, 3790, 4426, 4468
PspPI GGNCC 6 cut(s) 930, 1873, 2411, 2512, 2807, 4286
PspXI VCTCGAGB 1 cut(s) 3438
PsrI GAACNNNNNNTAC 2 cut(s) 1430, 1462
PstI CTGCAG 3 cut(s) 249, 1353, 4213
PstNI CAGNNNCTG 2 cut(s) 734, 1094
PsuI RGATCY 5 cut(s) 241, 409, 604, 1012, 1772
PsyI GACNNNGTC 1 cut(s) 3210
PvuII CAGCTG 1 cut(s) 4460
RseI CAYNNNNRTG 2 cut(s) 1431, 3154
SapI GCTCTTC 1 cut(s) 1956
Sau96I GGNCC 6 cut(s) 930, 1873, 2411, 2512, 2807, 4286
ScaI AGTACT 1 cut(s) 3577
SchI GAGTC 4 cut(s) 263, 287, 1744, 4048
SduI GDGCHC 6 cut(s) 1150, 2288, 2677, 3445, 3502, 4330
SfcI CTRYAG 8 cut(s) 105, 245, 414, 525, 1349, 1533, 2289, 4209
Sfr274I CTCGAG 1 cut(s) 3438
SfuI TTCGAA 2 cut(s) 143, 3194
SinI GGWCC 4 cut(s) 1873, 2411, 2512, 2807
SlaI CTCGAG 1 cut(s) 3438
SmiMI CAYNNNNRTG 2 cut(s) 1431, 3154
SmlI CTYRAG 5 cut(s) 2732, 3182, 3438, 4136, 4387
SmoI CTYRAG 5 cut(s) 2732, 3182, 3438, 4136, 4387
SpeI ACTAGT 2 cut(s) 182, 4265
SphI GCATGC 4 cut(s) 1580, 1817, 1898, 2692
SsiI CCGC 6 cut(s) 369, 2683, 2905, 3490, 4199, 4613
SspI AATATT 2 cut(s) 3191, 3404
StyI CCWWGG 2 cut(s) 1723, 2049
TaiI ACGT 4 cut(s) 2912, 3247, 4024, 4369
TaqI TCGA 8 cut(s) 63, 143, 400, 3194, 3439, 3821, 3957, 4149
TatI WGTACW 3 cut(s) 2107, 3575, 4672
TfiI GAWTC 9 cut(s) 815, 1055, 1912, 2621, 3049, 3113, 3959, 4559, 4724
TscAI CASTG 7 cut(s) 333, 471, 1250, 1365, 3759, 4260, 4876
TseFI GTSAC 6 cut(s) 823, 2329, 2983, 4017, 4035, 4567
Tsp45I GTSAC 6 cut(s) 823, 2329, 2983, 4017, 4035, 4567
TspGWI ACGGA 4 cut(s) 50, 808, 1242, 2348
TspRI CASTG 7 cut(s) 333, 471, 1250, 1365, 3759, 4260, 4876
Tth111I GACNNNGTC 1 cut(s) 3210
Van91I CCANNNNNTGG 3 cut(s) 3367, 3935, 4475
VpaK11BI GGWCC 4 cut(s) 1873, 2411, 2512, 2807
XapI RAATTY 8 cut(s) 664, 1024, 1273, 2715, 2989, 3526, 4115, 4318
XcmI CCANNNNNNNNNTGG 2 cut(s) 1430, 3509
XhoI CTCGAG 1 cut(s) 3438
XmiI GTMKAC 1 cut(s) 4596
XmnI GAANNNNTTC 2 cut(s) 3919, 4530
ZrmI AGTACT 1 cut(s) 3577
Zsp2I ATGCAT 3 cut(s) 1900, 2073, 2694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.