Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001382

Physical Location & Seq

Genomic Coordinates Forward (+)

28575406 .. 28580276

Gene Structure 4871 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1142000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGAAATCAAAACCACTAAAAACTCCGTCGGGCCTCCGACTGCGCCGCCTCACACGGTGTCAGTTCAGTTCCCCACAACCTTCCCTCCTCTGCAAAATACCCACAAAAATTAGTATACCAATCGCAAATGGCCGCCGTTTAAGAAATAGCAGAGAAAGAGAAAGCACCTCCCGAAGGAAAACCAGAAGCAGAGGAGGAAGAAGAAGACGAAGAGGAAGAGGAGCTTTGGACGTCGGAGATGCAGAGGACTACTTGGACTCCAAGGACGACGACAGAGGAGTAGAAGGCGGCTTCACCATCAATTCACGGCGGCCCAATGACCACGGCGGCCTTCACTCCCGCTCTGCTTCCTCTACCCTTCAGCCTCTCGCCAATCGCAACCAGAAGTTCACTACCCACATCCGAGCTTCGCTTTTGGAGGAGTGGGAAGGGAGGTTGAATGTTGGAATGTCCAACTCTGTGACAACTGCCATTCGGGGAAGTGTTAGGGACTTGGCGATTGGCAGAACGAAAACTACTGAGAAAGCGGACCGTGCCACTGTTGAGCAGGCCATCGATCCCAGAACTCGGATGGTTTTGTTCAAAATGTTGAACAGTGGTGTGTTTCATGATATCAATGGCTGTATATCTACCGGGAAAGAAGTACGTATTGAGTTGCTCGTGGACAGGGATAGATACGTACAAGGGGACTACCAATTCAGACATGGATACTGCAAGCGCAATCCTAGGAAAATGGTGCAGACATGGGCTGAGAAAGAGATGAGGAATTTGAAGAGGGAAGTCAGGTTGGGCAGCGCCTCGTCTGAAGGATGCTGCGTCATCACTAGACAAGCTTCGAGAAGGCTATATGGAGGTATGATTATTGCTATGCGAACAATGTATCAGAAGAGCAAACTGGTGCATGGAGACCTTAGTGAATACAACACACTTTACTTTGAGGGTCACTTGTACATTATCGACGTTTCTCAAGCAGTTGATTTGGACTACCCTCATGCCCTTGATTTCCTACGTGAAGATTGTGTCCATGTATCGGATTTCTTCAAAAAACATGGTGTAGCTGTTATGACAATTCGAGAGCTCTTTCATTTTATAGTCGATCCAACAATTGCTGATGATGCGGTGGAGAGTTATCTCGATATGCTACAACAAAAGGTTATGGCAAGAGGAGATATATCTGCAGAGGAGGAAATTGCAGACTCTGTATTTTCATACATTCCGAAGACATTAGATGACGTGAAGAATGCTGAGTGGGATGTAATACGGTTAACTAGCGGAGAGGACACTGGAGACATGTACTATAAAACGATTACAGGACTGAAGGAGGCTCTTCCTCAAGGTCAACCAGCTCCATCGGAGAAAGAGCAGCAACAAGATGCTATTGCTGTAGCAGAGCAGTCTGAAAATCCTGAGACTGAATCCGACTCTGATATGGATGACGAGAACTCAAGTGACTCGGAAGGGCAGAGTTCCTCTTCCGAAACCAAGGCACAGGATCCTCTCGAAAAGAAAGCTGCGAGAAAAGAGAACAAAAAGAAGGTGAAAGAAGAGAGGAGGGAAGCTCGGAAAACTAAAGTGCCGAAGGCTCTGAAGAAACGAAAGAAAAAGATGGCCAAAGCACAAAAGACCAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0016070 GO:0016072 GO:0019219 GO:0019222 GO:0022613 GO:0030490 GO:0030684 GO:0030688 GO:0031323 GO:0031325 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0034708 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090069 GO:0090070 GO:0090304 GO:1901360 GO:1902494 GO:1990234 GO:1990904 GO:2000232 GO:2000234

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

RIO1

Protein Analysis

544

Amino Acids

61.64

Weight (kDa)

9.45

Isoelectric Point (pI)

47.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RIO1	PF01163	222 - 259	5.1e-08	RIO domain
RIO1	PF01163	277 - 359	4e-27	RIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24990 AT5G37350 AT5G37350
fragaria_vesca	FvH4_5g08310 FvH4_5g08310 FvH4_5g08310 FvH4_5g16770 FvH4_5g16770 FvH4_5g16770 FvH4_5g25261
malus_domestica	MD06G1142000.v1.1 MD14G1157300.v1.1
prunus_persica	Prupe.5G151200_v2.0.a1
pyrus_communis	pycom06g13180 pycom14g13080
rosa_chinensis	RchiOBHm_Chr5g0020401 RchiOBHm_Chr5g0063241 RchiOBHm_Chr5g0063291 RchiOBHm_Chr7g0190211
rosa_laevigata	RLG00000004553 RLG00000032520 RLG00000035614 RLG00000035619
rosa_multiflora	Rmu_sc0000033.1_g000014 Rmu_sc0004886.1_g000019 Rmu_sc0005045.1_g000016
rosa_roxburghii	Rroxscaffold_1G00017410 Rroxscaffold_1G00017420 Rroxscaffold_1G00017470 Rroxscaffold_3G00264780
rosa_rugosa	Rorug05G0058900 Rorug05G0058900 Rorug05G0059000 Rorug05G0059100 Rorug05G0355500 Rorug06G0503000
rosa_samantha	Rh5AG149600 Rh5AG415200 Rh5AG415300 Rh5AG415500 Rh5BG149000 Rh5BG430400 Rh5CG160500 Rh5CG453800 Rh5DG147800 Rh5DG444000 Rh7AG108300 Rh7BG110900 Rh7CG113300 Rh7DG111700
rosa_wichuraiana	Rw5G013250 Rw5G039050 Rw7G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	234
AccBSI	CCGCTC	1 cut(s)	342
AccI	GTMKAC	1 cut(s)	115
AciI	CCGC	9 cut(s)	46, 133, 288, 310, 327, 340, 527, 1118, 1272
AclWI	GGATC	4 cut(s)	551, 1091, 1487, 1500
AcoI	YGGCCR	2 cut(s)	130, 1608
AcsI	RAATTY	1 cut(s)	766
AcuI	CTGAAG	4 cut(s)	344, 825, 1337, 1607
AcyI	GRCGYC	1 cut(s)	231
AdeI	CACNNNGTG	1 cut(s)	57
AfaI	GTAC	4 cut(s)	645, 681, 950, 1295
AfiI	CCNNNNNNNGG	3 cut(s)	174, 567, 1030
AflIII	ACRYGT	1 cut(s)	1290
AgsI	TTSAA	5 cut(s)	439, 583, 592, 772, 1042
AjiI	CACGTC	1 cut(s)	1234
AjnI	CCWGG	1 cut(s)	1625
AluBI	AGCT	8 cut(s)	224, 407, 833, 1058, 1078, 1346, 1511, 1559
AluI	AGCT	8 cut(s)	224, 407, 833, 1058, 1078, 1346, 1511, 1559
Alw21I	GWGCWC	1 cut(s)	1080
Alw26I	GTCTC	3 cut(s)	900, 1281, 1403
AlwI	GGATC	4 cut(s)	551, 1091, 1487, 1500
AlwNI	CAGNNNCTG	1 cut(s)	1199
AoxI	GGCC	6 cut(s)	31, 130, 311, 328, 549, 1608
ApeKI	GCWGC	4 cut(s)	792, 813, 1363, 1511
ApoI	RAATTY	1 cut(s)	766
AspA2I	CCTAGG	1 cut(s)	725
AspLEI	GCGC	3 cut(s)	45, 720, 797
AspS9I	GGNCC	3 cut(s)	31, 312, 529
AsuC2I	CCSGG	1 cut(s)	634
AsuHPI	GGTGA	2 cut(s)	286, 1549
AvaII	GGWCC	1 cut(s)	529
AvrII	CCTAGG	1 cut(s)	725
BalI	TGGCCA	1 cut(s)	1610
BamHI	GGATCC	1 cut(s)	1492
BanII	GRGCYC	1 cut(s)	1080
BauI	CACGAG	1 cut(s)	659
BbsI	GAAGAC	2 cut(s)	211, 1226
Bbv12I	GWGCWC	1 cut(s)	1080
BbvI	GCAGC	4 cut(s)	800, 804, 1375, 1498
BccI	CCATC	5 cut(s)	305, 560, 565, 1357, 1600
BceAI	ACGGC	3 cut(s)	120, 323, 340
BciT130I	CCWGG	1 cut(s)	1627
BciVI	GTATCC	1 cut(s)	701
BcnI	CCSGG	1 cut(s)	634
BcoDI	GTCTC	3 cut(s)	900, 1281, 1403
BfaI	CTAG	3 cut(s)	726, 825, 1269
BfmI	CTRYAG	2 cut(s)	1176, 1383
BfoI	RGCGCY	1 cut(s)	798
BfuI	GTATCC	1 cut(s)	701
BisI	GCNGC	9 cut(s)	46, 133, 289, 311, 328, 793, 814, 1364, 1512
BlnI	CCTAGG	1 cut(s)	725
BlsI	GCNGC	9 cut(s)	47, 134, 290, 312, 329, 794, 815, 1365, 1513
Bme1390I	CCNGG	2 cut(s)	634, 1627
Bme18I	GGWCC	1 cut(s)	529
BmgBI	CACGTC	1 cut(s)	1234
BmgT120I	GGNCC	3 cut(s)	31, 312, 529
BmiI	GGNNCC	1 cut(s)	1494
BmrFI	CCNGG	2 cut(s)	634, 1627
BmsI	GCATC	4 cut(s)	229, 800, 1105, 1363
BpiI	GAAGAC	2 cut(s)	211, 1226
BplI	GAGNNNNNCTC	2 cut(s)	1543, 1575
BpmI	CTGGAG	1 cut(s)	1305
BpuEI	CTTGAG	3 cut(s)	951, 1317, 1429
BpuMI	CCSGG	1 cut(s)	634
Bsa29I	ATCGAT	1 cut(s)	555
BsaAI	YACGTR	3 cut(s)	647, 679, 1010
BsaHI	GRCGYC	1 cut(s)	231
BsaI	GGTCTC	1 cut(s)	900
BsaJI	CCNNGG	4 cut(s)	261, 322, 725, 1482
BsaXI	ACNNNNNCTCC	4 cut(s)	69, 99, 242, 272
Bsc4I	CCNNNNNNNGG	3 cut(s)	174, 567, 1030
Bse1I	ACTGG	2 cut(s)	900, 1288
BseBI	CCWGG	1 cut(s)	1627
BseCI	ATCGAT	1 cut(s)	555
BseDI	CCNNGG	4 cut(s)	261, 322, 725, 1482
BseGI	GGATG	5 cut(s)	399, 576, 815, 1258, 1438
BseLI	CCNNNNNNNGG	3 cut(s)	174, 567, 1030
BseMII	CTCAG	4 cut(s)	510, 741, 1236, 1398
BseNI	ACTGG	2 cut(s)	900, 1288
BseRI	GAGGAG	8 cut(s)	77, 207, 234, 291, 434, 1179, 1196, 1564
BseXI	GCAGC	4 cut(s)	800, 804, 1375, 1498
BsgI	GTGCAG	1 cut(s)	758
BshFI	GGCC	6 cut(s)	33, 132, 313, 330, 551, 1610
BshVI	ATCGAT	1 cut(s)	555
BsiHKAI	GWGCWC	1 cut(s)	1080
BsiSI	CCGG	1 cut(s)	633
BslFI	GGGAC	2 cut(s)	503, 701
BslI	CCNNNNNNNGG	3 cut(s)	174, 567, 1030
BsmAI	GTCTC	3 cut(s)	900, 1281, 1403
BsmFI	GGGAC	2 cut(s)	503, 701
BsmI	GAATGC	1 cut(s)	1246
BsnI	GGCC	6 cut(s)	33, 132, 313, 330, 551, 1610
Bso31I	GGTCTC	1 cut(s)	900
Bsp1286I	GDGCHC	1 cut(s)	1080
Bsp1407I	TGTACA	1 cut(s)	948
Bsp143I	GATC	3 cut(s)	556, 1096, 1492
BspACI	CCGC	9 cut(s)	46, 133, 288, 310, 327, 340, 527, 1118, 1272
BspANI	GGCC	6 cut(s)	33, 132, 313, 330, 551, 1610
BspCNI	CTCAG	4 cut(s)	511, 742, 1237, 1399
BspDI	ATCGAT	1 cut(s)	555
BspHI	TCATGA	1 cut(s)	607
BspLI	GGNNCC	1 cut(s)	1494
BspMAI	CTGCAG	1 cut(s)	1180
BspPI	GGATC	4 cut(s)	551, 1091, 1487, 1500
BspQI	GCTCTTC	2 cut(s)	881, 1332
BspTNI	GGTCTC	1 cut(s)	900
BsrBI	CCGCTC	1 cut(s)	342
BsrGI	TGTACA	1 cut(s)	948
BsrI	ACTGG	2 cut(s)	900, 1288
BssECI	CCNNGG	4 cut(s)	261, 322, 725, 1482
BssMI	GATC	3 cut(s)	556, 1096, 1492
BssNAI	GTATAC	1 cut(s)	116
BssNI	GRCGYC	1 cut(s)	231
BssSI	CACGAG	1 cut(s)	659
BssT1I	CCWWGG	3 cut(s)	261, 725, 1482
Bst1107I	GTATAC	1 cut(s)	116
Bst2BI	CACGAG	1 cut(s)	659
Bst2UI	CCWGG	1 cut(s)	1627
Bst4CI	ACNGT	5 cut(s)	57, 533, 541, 596, 1263
Bst6I	CTCTTC	7 cut(s)	205, 211, 767, 881, 1332, 1477, 1539
BstACI	GRCGYC	1 cut(s)	231
BstAUI	TGTACA	1 cut(s)	948
BstBAI	YACGTR	3 cut(s)	647, 679, 1010
BstC8I	GCNNGC	2 cut(s)	549, 716
BstDEI	CTNAG	5 cut(s)	519, 750, 911, 1245, 1407
BstDSI	CCRYGG	1 cut(s)	322
BstENI	CCTNNNNNAGG	1 cut(s)	172
BstF5I	GGATG	5 cut(s)	399, 576, 815, 1258, 1438
BstH2I	RGCGCY	1 cut(s)	798
BstHHI	GCGC	3 cut(s)	45, 720, 797
BstKTI	GATC	3 cut(s)	559, 1099, 1495
BstMAI	GTCTC	3 cut(s)	900, 1281, 1403
BstMBI	GATC	3 cut(s)	556, 1096, 1492
BstMWI	GCNNNNNNNGC	2 cut(s)	533, 1115
BstNI	CCWGG	1 cut(s)	1627
BstNSI	RCATGY	1 cut(s)	1294
BstSCI	CCNGG	2 cut(s)	632, 1625
BstSFI	CTRYAG	2 cut(s)	1176, 1383
BstSNI	TACGTA	2 cut(s)	647, 679
BstV1I	GCAGC	4 cut(s)	800, 804, 1375, 1498
BstV2I	GAAGAC	2 cut(s)	211, 1226
BstX2I	RGATCY	1 cut(s)	1492
BstYI	RGATCY	1 cut(s)	1492
BstZ17I	GTATAC	1 cut(s)	116
Bsu15I	ATCGAT	1 cut(s)	555
BsuI	GTATCC	1 cut(s)	701
BsuRI	GGCC	6 cut(s)	33, 132, 313, 330, 551, 1610
BsuTUI	ATCGAT	1 cut(s)	555
BtgI	CCRYGG	1 cut(s)	322
BtrI	CACGTC	1 cut(s)	1234
BtsCI	GGATG	5 cut(s)	399, 576, 815, 1258, 1438
BtsIMutI	CAGTG	3 cut(s)	537, 601, 1281
Cac8I	GCNNGC	2 cut(s)	549, 716
CaiI	CAGNNNCTG	1 cut(s)	1199
CciI	TCATGA	1 cut(s)	607
CfoI	GCGC	3 cut(s)	45, 720, 797
Cfr13I	GGNCC	3 cut(s)	31, 312, 529
ClaI	ATCGAT	1 cut(s)	555
CpoI	CGGWCCG	1 cut(s)	529
CseI	GACGC	1 cut(s)	805
CsiI	ACCWGGT	1 cut(s)	1625
Csp6I	GTAC	4 cut(s)	644, 680, 949, 1294
CspI	CGGWCCG	1 cut(s)	529
CviAII	CATG	8 cut(s)	608, 704, 744, 902, 992, 1025, 1049, 1291
CviQI	GTAC	4 cut(s)	644, 680, 949, 1294
DdeI	CTNAG	5 cut(s)	519, 750, 911, 1245, 1407
DpnI	GATC	3 cut(s)	558, 1098, 1494
DpnII	GATC	3 cut(s)	556, 1096, 1492
DraIII	CACNNNGTG	1 cut(s)	57
EaeI	YGGCCR	2 cut(s)	130, 1608
Eam1104I	CTCTTC	7 cut(s)	205, 211, 767, 881, 1332, 1477, 1539
EarI	CTCTTC	7 cut(s)	205, 211, 767, 881, 1332, 1477, 1539
Ecl136II	GAGCTC	1 cut(s)	1078
Eco105I	TACGTA	2 cut(s)	647, 679
Eco130I	CCWWGG	3 cut(s)	261, 725, 1482
Eco24I	GRGCYC	1 cut(s)	1080
Eco31I	GGTCTC	1 cut(s)	900
Eco32I	GATATC	1 cut(s)	613
Eco47I	GGWCC	1 cut(s)	529
Eco53kI	GAGCTC	1 cut(s)	1078
Eco57I	CTGAAG	4 cut(s)	344, 825, 1337, 1607
EcoICRI	GAGCTC	1 cut(s)	1078
EcoNI	CCTNNNNNAGG	1 cut(s)	172
EcoRII	CCWGG	1 cut(s)	1625
EcoRV	GATATC	1 cut(s)	613
EcoT14I	CCWWGG	3 cut(s)	261, 725, 1482
EcoT38I	GRGCYC	1 cut(s)	1080
ErhI	CCWWGG	3 cut(s)	261, 725, 1482
FaeI	CATG	8 cut(s)	611, 707, 747, 905, 995, 1028, 1052, 1294
FalI	AAGNNNNNCTT	2 cut(s)	208, 240
FaqI	GGGAC	2 cut(s)	503, 701
FatI	CATG	8 cut(s)	607, 703, 743, 901, 991, 1024, 1048, 1290
FauI	CCCGC	1 cut(s)	347
FblI	GTMKAC	1 cut(s)	115
Fnu4HI	GCNGC	9 cut(s)	46, 133, 289, 311, 328, 793, 814, 1364, 1512
FokI	GGATG	5 cut(s)	386, 583, 822, 1265, 1445
FriOI	GRGCYC	1 cut(s)	1080
Fsp4HI	GCNGC	9 cut(s)	46, 133, 289, 311, 328, 793, 814, 1364, 1512
FspBI	CTAG	3 cut(s)	726, 825, 1269
GlaI	GCGC	3 cut(s)	44, 719, 796
GluI	GCNGC	9 cut(s)	46, 133, 289, 311, 328, 793, 814, 1364, 1512
GsuI	CTGGAG	1 cut(s)	1305
HaeII	RGCGCY	1 cut(s)	798
HaeIII	GGCC	6 cut(s)	33, 132, 313, 330, 551, 1610
HapII	CCGG	1 cut(s)	633
HgaI	GACGC	1 cut(s)	805
HhaI	GCGC	3 cut(s)	45, 720, 797
Hin1I	GRCGYC	1 cut(s)	231
Hin1II	CATG	8 cut(s)	611, 707, 747, 905, 995, 1028, 1052, 1294
Hin6I	GCGC	3 cut(s)	43, 718, 795
HinP1I	GCGC	3 cut(s)	43, 718, 795
HincII	GTYRAC	2 cut(s)	1266, 1340
HindII	GTYRAC	2 cut(s)	1266, 1340
HindIII	AAGCTT	1 cut(s)	831
HinfI	GANTC	5 cut(s)	257, 1196, 1415, 1421, 1451
HpaI	GTTAAC	1 cut(s)	1266
HpaII	CCGG	1 cut(s)	633
HphI	GGTGA	2 cut(s)	286, 1549
Hpy166II	GTNNAC	5 cut(s)	116, 390, 664, 1266, 1340
Hpy188III	TCNNGA	7 cut(s)	171, 608, 837, 1073, 1133, 1406, 1499
Hpy8I	GTNNAC	5 cut(s)	116, 390, 664, 1266, 1340
Hpy99I	CGWCG	4 cut(s)	31, 236, 272, 962
HpyCH4III	ACNGT	5 cut(s)	57, 533, 541, 596, 1263
HpyCH4IV	ACGT	6 cut(s)	231, 646, 678, 960, 1009, 1233
HpyCH4V	TGCA	7 cut(s)	93, 242, 714, 739, 901, 1178, 1193
HpyF10VI	GCNNNNNNNGC	2 cut(s)	533, 1115
HpyF3I	CTNAG	5 cut(s)	519, 750, 911, 1245, 1407
HpySE526I	ACGT	6 cut(s)	231, 646, 678, 960, 1009, 1233
Hsp92I	GRCGYC	1 cut(s)	231
Hsp92II	CATG	8 cut(s)	611, 707, 747, 905, 995, 1028, 1052, 1294
HspAI	GCGC	3 cut(s)	43, 718, 795
KspAI	GTTAAC	1 cut(s)	1266
Kzo9I	GATC	3 cut(s)	556, 1096, 1492
LguI	GCTCTTC	2 cut(s)	881, 1332
LmnI	GCTCC	2 cut(s)	221, 1351
Lsp1109I	GCAGC	4 cut(s)	800, 804, 1375, 1498
LweI	GCATC	4 cut(s)	229, 800, 1105, 1363
MabI	ACCWGGT	1 cut(s)	1625
MaeI	CTAG	3 cut(s)	726, 825, 1269
MaeII	ACGT	6 cut(s)	231, 646, 678, 960, 1009, 1233
MaeIII	GTNAC	3 cut(s)	460, 941, 1448
MalI	GATC	3 cut(s)	558, 1098, 1494
MbiI	CCGCTC	1 cut(s)	342
MboI	GATC	3 cut(s)	556, 1096, 1492
MfeI	CAATTG	1 cut(s)	1104
MflI	RGATCY	1 cut(s)	1492
MhlI	GDGCHC	1 cut(s)	1080
MlsI	TGGCCA	1 cut(s)	1610
MluCI	AATT	7 cut(s)	108, 301, 695, 766, 1068, 1104, 1188
MluNI	TGGCCA	1 cut(s)	1610
MlyI	GAGTC	4 cut(s)	251, 1190, 1415, 1445
MmeI	TCCRAC	6 cut(s)	61, 214, 424, 477, 1124, 1443
Mox20I	TGGCCA	1 cut(s)	1610
MscI	TGGCCA	1 cut(s)	1610
MseI	TTAA	2 cut(s)	140, 1265
Msp20I	TGGCCA	1 cut(s)	1610
MspI	CCGG	1 cut(s)	633
MspR9I	CCNGG	2 cut(s)	634, 1627
MunI	CAATTG	1 cut(s)	1104
Mva1269I	GAATGC	1 cut(s)	1246
MvaI	CCWGG	1 cut(s)	1627
MwoI	GCNNNNNNNGC	2 cut(s)	533, 1115
NciI	CCSGG	1 cut(s)	634
NdeII	GATC	3 cut(s)	556, 1096, 1492
NlaIII	CATG	8 cut(s)	611, 707, 747, 905, 995, 1028, 1052, 1294
NlaIV	GGNNCC	1 cut(s)	1494
NmuCI	GTSAC	3 cut(s)	460, 941, 1448
NspI	RCATGY	1 cut(s)	1294
PagI	TCATGA	1 cut(s)	607
PciI	ACATGT	1 cut(s)	1290
PciSI	GCTCTTC	2 cut(s)	881, 1332
PctI	GAATGC	1 cut(s)	1246
PfeI	GAWTC	1 cut(s)	1415
PkrI	GCNGC	9 cut(s)	47, 134, 290, 312, 329, 794, 815, 1365, 1513
PleI	GAGTC	4 cut(s)	251, 1190, 1415, 1445
PpsI	GAGTC	4 cut(s)	251, 1190, 1415, 1445
Ppu21I	YACGTR	3 cut(s)	647, 679, 1010
PscI	ACATGT	1 cut(s)	1290
Psp124BI	GAGCTC	1 cut(s)	1080
Psp6I	CCWGG	1 cut(s)	1625
PspGI	CCWGG	1 cut(s)	1625
PspN4I	GGNNCC	1 cut(s)	1494
PspPI	GGNCC	3 cut(s)	31, 312, 529
PsrI	GAACNNNNNNTAC	2 cut(s)	499, 531
PstI	CTGCAG	1 cut(s)	1180
PstNI	CAGNNNCTG	1 cut(s)	1199
PsuI	RGATCY	1 cut(s)	1492
RsaI	GTAC	4 cut(s)	645, 681, 950, 1295
RsaNI	GTAC	4 cut(s)	644, 680, 949, 1294
Rsr2I	CGGWCCG	1 cut(s)	529
RsrII	CGGWCCG	1 cut(s)	529
SacI	GAGCTC	1 cut(s)	1080
SapI	GCTCTTC	2 cut(s)	881, 1332
SaqAI	TTAA	2 cut(s)	140, 1265
SatI	GCNGC	9 cut(s)	46, 133, 289, 311, 328, 793, 814, 1364, 1512
Sau3AI	GATC	3 cut(s)	556, 1096, 1492
Sau96I	GGNCC	3 cut(s)	31, 312, 529
SchI	GAGTC	4 cut(s)	251, 1190, 1415, 1445
ScrFI	CCNGG	2 cut(s)	634, 1627
SduI	GDGCHC	1 cut(s)	1080
SexAI	ACCWGGT	1 cut(s)	1625
SfaNI	GCATC	4 cut(s)	229, 800, 1105, 1363
SfcI	CTRYAG	2 cut(s)	1176, 1383
SinI	GGWCC	1 cut(s)	529
SmlI	CTYRAG	3 cut(s)	966, 1332, 1444
SmoI	CTYRAG	3 cut(s)	966, 1332, 1444
SnaBI	TACGTA	2 cut(s)	647, 679
Sse9I	AATT	7 cut(s)	108, 301, 695, 766, 1068, 1104, 1188
SsiI	CCGC	9 cut(s)	46, 133, 288, 310, 327, 340, 527, 1118, 1272
SspMI	CTAG	3 cut(s)	726, 825, 1269
SstI	GAGCTC	1 cut(s)	1080
StyD4I	CCNGG	2 cut(s)	632, 1625
StyI	CCWWGG	3 cut(s)	261, 725, 1482
TaaI	ACNGT	5 cut(s)	57, 533, 541, 596, 1263
TaiI	ACGT	6 cut(s)	234, 649, 681, 963, 1012, 1236
TaqI	TCGA	7 cut(s)	555, 836, 957, 1072, 1095, 1134, 1500
TasI	AATT	7 cut(s)	108, 301, 695, 766, 1068, 1104, 1188
TatI	WGTACW	2 cut(s)	948, 1293
TauI	GCSGC	5 cut(s)	48, 135, 291, 313, 330
TfiI	GAWTC	1 cut(s)	1415
Tru1I	TTAA	2 cut(s)	140, 1265
Tru9I	TTAA	2 cut(s)	140, 1265
TscAI	CASTG	3 cut(s)	544, 601, 1288
TseFI	GTSAC	3 cut(s)	460, 941, 1448
TseI	GCWGC	4 cut(s)	792, 813, 1363, 1511
Tsp45I	GTSAC	3 cut(s)	460, 941, 1448
TspDTI	ATGAA	4 cut(s)	17, 596, 1073, 1197
TspGWI	ACGGA	1 cut(s)	15
TspRI	CASTG	3 cut(s)	544, 601, 1288
VpaK11BI	GGWCC	1 cut(s)	529
XagI	CCTNNNNNAGG	1 cut(s)	172
XapI	RAATTY	1 cut(s)	766
XceI	RCATGY	1 cut(s)	1294
XcmI	CCANNNNNNNNNTGG	2 cut(s)	568, 701
XmaJI	CCTAGG	1 cut(s)	725
XmiI	GTMKAC	1 cut(s)	115
XspI	CTAG	3 cut(s)	726, 825, 1269
ZraI	GACGTC	1 cut(s)	232

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.