Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0001382

Physical Location & Seq

Genomic Coordinates Forward (+)

68995572 .. 69004961

Gene Structure 9390 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G039050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1440 bp

ATGGATGTCGTCTATGCTCTCAAGAGGCAGGGAAGGACCCTTTACGGTTTCGGAGCGTGTTTTCCAATCAACTATGCTAGCCAGCGACCCAATGCTCATGGAGGCCTACACTCTCGCCCTAATAACTCCAACCTTCGTCGTTTCTCTAACCTCGTCAGACTTTCGCCTCTAGAGGAGTGGGAAGGCAGGTTGAATATAGACATGTCCAACTATCTCATAACTTCCATTGGCAGCAGCATTAGGGTTAAAGATAAAGCCGGTCGTGCCACTTTTGGCAAGGGTTTTGATCTTACAACTTCTTTGGCTATGCGGAAACTGCACAATCGCGGTGAATATGAGTTTATCCATGGCTGCATTTCTACGGGGAAAGATGCAAATGTTTATTACGCAACAAGATCTGATGGCCAACAAGATGTGTGTTACCCAACAAGATCTGGTGACCAGGACCTGGCTATCAAAGTGTACAAAACATCTGTTATGGGTTTTAAGCACAGAGGTGGATATGTACAAGGGGACTACCGATTCAGACATGAATACAGTGGGCACAATCCCAGGAAACAGGTGAGCAAATGGGTTGAGAAAGAGTGGAGGAATCTGAAGAGGATAAAGGAAGCTGGAATCAGATGCCCAACTGCATATGACTTTGGACCTATTGTTCTGGTCATGGAATTCATAGGGAAGGCAGGTTGTGCAGCACCTCGTTTGAAGGATGCCACATTGTCATTACACGAGCTACGAGAAGGATATAGGGAGATGATTATTTCTATGCGAATGCTATATCAGAAGGCCAAACTGGTGCATGCAGACCTCAGCGAAGACAACGTACTTTATTTTGAAGGCCATTTGTACATGATTGATGTTTCTCAAGCAGTTGAGCTAAACGACCCTAGTGCGAATGGATTTCTATGTAAAGATTGTCTCTATGTCTCTGATTTCTTTAAAAAACATGGAGCAGCTGTTATGACAACACGGGAGCTCTTTGATTTTATTGTTGATCCATGTATTTCGGATGACATGGTGGACAGTTATCTGAATATGTTGTGGCAAGGGGATCTATATCTGCAGAGGATGAAATTGCAGACTCTGTGTTTGTGCAGTTTAGAAAATTTGAAGAATGTTGAGGCGGATGCAAATCATGCAATACAGATAACTAGTGATAAGGACACTGAAGAGATGGACTATAAAACTATTACTGCACTGGAGCAGGCACTTTCTAGAGTCCAACTAGCTCCACTTGAGACCAATTCTGATGAAGAAAACTCAAGCGATTTAGATTCCTCATCTGGAATCAAGGCACAGAACAAGAAGGTTGCGAGAAAATATTTCAAGAAGAAAGTCAAAGAAGTGAAGATGGAATCTCGGAAACATGAATTGCAAAAGCGCCCAAAGGCTGTAAAGAAAAGAAAGAAGAGGCTGTCGGAACCTTGCAGGACCAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0016070 GO:0016072 GO:0019219 GO:0019222 GO:0022613 GO:0030490 GO:0030684 GO:0030688 GO:0031323 GO:0031325 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0034708 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090069 GO:0090070 GO:0090304 GO:1901360 GO:1902494 GO:1990234 GO:1990904 GO:2000232 GO:2000234

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

RIO1

Protein Analysis

479

Amino Acids

54.84

Weight (kDa)

9.18

Isoelectric Point (pI)

33.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RIO1	PF01163	126 - 325	3.3e-57	RIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24990 AT5G37350 AT5G37350
fragaria_vesca	FvH4_5g08310 FvH4_5g08310 FvH4_5g08310 FvH4_5g16770 FvH4_5g16770 FvH4_5g16770 FvH4_5g25261
malus_domestica	MD06G1142000.v1.1 MD14G1157300.v1.1
prunus_persica	Prupe.5G151200_v2.0.a1
pyrus_communis	pycom06g13180 pycom14g13080
rosa_chinensis	RchiOBHm_Chr5g0020401 RchiOBHm_Chr5g0063241 RchiOBHm_Chr5g0063291 RchiOBHm_Chr7g0190211
rosa_laevigata	RLG00000004553 RLG00000032520 RLG00000035614 RLG00000035619
rosa_multiflora	Rmu_sc0000033.1_g000014 Rmu_sc0004886.1_g000019 Rmu_sc0005045.1_g000016
rosa_roxburghii	Rroxscaffold_1G00017410 Rroxscaffold_1G00017420 Rroxscaffold_1G00017470 Rroxscaffold_3G00264780
rosa_rugosa	Rorug05G0058900 Rorug05G0058900 Rorug05G0059000 Rorug05G0059100 Rorug05G0355500 Rorug06G0503000
rosa_samantha	Rh5AG149600 Rh5AG415200 Rh5AG415300 Rh5AG415500 Rh5BG149000 Rh5BG430400 Rh5CG160500 Rh5CG453800 Rh5DG147800 Rh5DG444000 Rh7AG108300 Rh7BG110900 Rh7CG113300 Rh7DG111700
rosa_wichuraiana	Rw5G013250 Rw5G039050 Rw7G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	177, 674
AccB7I	CCANNNNNTGG	1 cut(s)	448
AccII	CGCG	1 cut(s)	327
AciI	CCGC	3 cut(s)	310, 327, 1124
AclWI	GGATC	2 cut(s)	989, 1059
AcoI	YGGCCR	1 cut(s)	403
AcsI	RAATTY	2 cut(s)	668, 1105
AcuI	CTGAAG	2 cut(s)	617, 1188
AfaI	GTAC	4 cut(s)	464, 507, 825, 848
AfiI	CCNNNNNNNGG	1 cut(s)	448
AflIII	ACRYGT	1 cut(s)	201
AgsI	TTSAA	5 cut(s)	193, 706, 836, 1111, 1327
AhlI	ACTAGT	1 cut(s)	1151
AjnI	CCWGG	4 cut(s)	441, 447, 551, 1433
AleI	CACNNNNGTG	1 cut(s)	495
AloI	GAACNNNNNNTCC	2 cut(s)	639, 671
AluBI	AGCT	6 cut(s)	614, 733, 877, 956, 976, 1229
AluI	AGCT	6 cut(s)	614, 733, 877, 956, 976, 1229
Alw21I	GWGCWC	1 cut(s)	978
Alw26I	GTCTC	3 cut(s)	923, 931, 1232
AlwI	GGATC	2 cut(s)	989, 1059
AlwNI	CAGNNNCTG	2 cut(s)	448, 1084
AoxI	GGCC	4 cut(s)	103, 403, 786, 838
ApeKI	GCWGC	5 cut(s)	231, 234, 351, 692, 953
ApoI	RAATTY	2 cut(s)	668, 1105
ArsI	GACNNNNNNTTYG	2 cut(s)	1072, 1104
AspLEI	GCGC	1 cut(s)	1383
AspS9I	GGNCC	4 cut(s)	36, 445, 647, 1431
AsuHPI	GGTGA	3 cut(s)	341, 449, 574
AsuNHI	GCTAGC	1 cut(s)	77
AvaII	GGWCC	4 cut(s)	36, 445, 647, 1431
BaeGI	GKGCMC	1 cut(s)	546
BalI	TGGCCA	1 cut(s)	405
BanII	GRGCYC	1 cut(s)	978
BarI	GAAGNNNNNNTAC	2 cut(s)	807, 839
BauI	CACGAG	1 cut(s)	728
BbsI	GAAGAC	1 cut(s)	822
Bbv12I	GWGCWC	1 cut(s)	978
BbvCI	CCTCAGC	1 cut(s)	809
BbvI	GCAGC	5 cut(s)	243, 246, 338, 704, 965
BccI	CCATC	3 cut(s)	395, 1168, 1345
BcgI	CGANNNNNNTGC	2 cut(s)	872, 906
BciT130I	CCWGG	4 cut(s)	443, 449, 553, 1435
BcoDI	GTCTC	3 cut(s)	923, 931, 1232
BcuI	ACTAGT	1 cut(s)	1151
BfaI	CTAG	6 cut(s)	78, 170, 888, 1152, 1215, 1226
BfmI	CTRYAG	1 cut(s)	1061
BfoI	RGCGCY	1 cut(s)	1384
BfuAI	ACCTGC	2 cut(s)	177, 674
BglII	AGATCT	2 cut(s)	395, 431
BisI	GCNGC	5 cut(s)	232, 235, 352, 693, 954
BlsI	GCNGC	5 cut(s)	233, 236, 353, 694, 955
Bme1390I	CCNGG	4 cut(s)	443, 449, 553, 1435
Bme18I	GGWCC	4 cut(s)	36, 445, 647, 1431
BmgT120I	GGNCC	4 cut(s)	36, 445, 647, 1431
BmiI	GGNNCC	2 cut(s)	38, 1422
BmrFI	CCNGG	4 cut(s)	443, 449, 553, 1435
BmsI	GCATC	4 cut(s)	361, 614, 700, 1117
BmtI	GCTAGC	1 cut(s)	81
BpiI	GAAGAC	1 cut(s)	822
BpmI	CTGGAG	1 cut(s)	1220
Bpu10I	CCTNAGC	1 cut(s)	809
BpuEI	CTTGAG	4 cut(s)	5, 849, 1246, 1256
BsaBI	GATNNNNATC	2 cut(s)	1056, 1131
BsaI	GGTCTC	1 cut(s)	1232
BsaJI	CCNNGG	2 cut(s)	346, 551
BsaXI	ACNNNNNCTCC	2 cut(s)	942, 972
Bsc4I	CCNNNNNNNGG	1 cut(s)	448
Bse118I	RCCGGY	1 cut(s)	257
Bse1I	ACTGG	2 cut(s)	798, 1203
Bse8I	GATNNNNATC	2 cut(s)	1056, 1131
BseBI	CCWGG	4 cut(s)	443, 449, 553, 1435
BseDI	CCNNGG	2 cut(s)	346, 551
BseGI	GGATG	5 cut(s)	10, 715, 1015, 1074, 1132
BseJI	GATNNNNATC	2 cut(s)	1056, 1131
BseLI	CCNNNNNNNGG	1 cut(s)	448
BseMII	CTCAG	1 cut(s)	823
BseNI	ACTGG	2 cut(s)	798, 1203
BseRI	GAGGAG	1 cut(s)	188
BseSI	GKGCMC	1 cut(s)	546
BseXI	GCAGC	5 cut(s)	243, 246, 338, 704, 965
BsgI	GTGCAG	4 cut(s)	302, 711, 1114, 1179
Bsh1236I	CGCG	1 cut(s)	327
Bsh1285I	CGRYCG	1 cut(s)	262
BshFI	GGCC	4 cut(s)	105, 405, 788, 840
BsiEI	CGRYCG	1 cut(s)	262
BsiHKAI	GWGCWC	1 cut(s)	978
BsiSI	CCGG	1 cut(s)	258
BslFI	GGGAC	1 cut(s)	527
BslI	CCNNNNNNNGG	1 cut(s)	448
BsmAI	GTCTC	3 cut(s)	923, 931, 1232
BsmFI	GGGAC	1 cut(s)	527
BsmI	GAATGC	1 cut(s)	777
BsnI	GGCC	4 cut(s)	105, 405, 788, 840
Bso31I	GGTCTC	1 cut(s)	1232
Bsp1286I	GDGCHC	2 cut(s)	546, 978
Bsp1407I	TGTACA	3 cut(s)	462, 505, 846
Bsp143I	GATC	5 cut(s)	286, 395, 431, 994, 1051
Bsp19I	CCATGG	1 cut(s)	346
BspACI	CCGC	3 cut(s)	310, 327, 1124
BspANI	GGCC	4 cut(s)	105, 405, 788, 840
BspCNI	CTCAG	1 cut(s)	822
BspFNI	CGCG	1 cut(s)	327
BspLI	GGNNCC	2 cut(s)	38, 1422
BspMAI	CTGCAG	1 cut(s)	1065
BspMI	ACCTGC	2 cut(s)	177, 674
BspOI	GCTAGC	1 cut(s)	81
BspPI	GGATC	2 cut(s)	989, 1059
BspTNI	GGTCTC	1 cut(s)	1232
BsrFI	RCCGGY	1 cut(s)	257
BsrGI	TGTACA	3 cut(s)	462, 505, 846
BsrI	ACTGG	2 cut(s)	798, 1203
BssAI	RCCGGY	1 cut(s)	257
BssECI	CCNNGG	2 cut(s)	346, 551
BssMI	GATC	5 cut(s)	286, 395, 431, 994, 1051
BssSI	CACGAG	1 cut(s)	728
BssT1I	CCWWGG	1 cut(s)	346
Bst2BI	CACGAG	1 cut(s)	728
Bst2UI	CCWGG	4 cut(s)	443, 449, 553, 1435
Bst4CI	ACNGT	3 cut(s)	47, 539, 1025
Bst6I	CTCTTC	3 cut(s)	593, 1164, 1403
BstAPI	GCANNNNNTGC	2 cut(s)	689, 1136
BstAUI	TGTACA	3 cut(s)	462, 505, 846
BstC8I	GCNNGC	4 cut(s)	79, 83, 801, 1206
BstDEI	CTNAG	1 cut(s)	809
BstDSI	CCRYGG	1 cut(s)	346
BstEII	GGTNACC	1 cut(s)	437
BstF5I	GGATG	5 cut(s)	10, 715, 1015, 1074, 1132
BstFNI	CGCG	1 cut(s)	327
BstH2I	RGCGCY	1 cut(s)	1384
BstHHI	GCGC	1 cut(s)	1383
BstKTI	GATC	5 cut(s)	289, 398, 434, 997, 1054
BstMAI	GTCTC	3 cut(s)	923, 931, 1232
BstMBI	GATC	5 cut(s)	286, 395, 431, 994, 1051
BstMCI	CGRYCG	1 cut(s)	262
BstMWI	GCNNNNNNNGC	4 cut(s)	263, 316, 689, 1136
BstNI	CCWGG	4 cut(s)	443, 449, 553, 1435
BstNSI	RCATGY	2 cut(s)	205, 803
BstPI	GGTNACC	1 cut(s)	437
BstSCI	CCNGG	4 cut(s)	441, 447, 551, 1433
BstSFI	CTRYAG	1 cut(s)	1061
BstSLI	GKGCMC	1 cut(s)	546
BstUI	CGCG	1 cut(s)	327
BstV1I	GCAGC	5 cut(s)	243, 246, 338, 704, 965
BstV2I	GAAGAC	1 cut(s)	822
BstX2I	RGATCY	3 cut(s)	395, 431, 1051
BstYI	RGATCY	3 cut(s)	395, 431, 1051
BsuRI	GGCC	4 cut(s)	105, 405, 788, 840
BtgI	CCRYGG	1 cut(s)	346
BtsCI	GGATG	5 cut(s)	10, 715, 1015, 1074, 1132
BtsIMutI	CAGTG	3 cut(s)	544, 1164, 1196
BveI	ACCTGC	2 cut(s)	177, 674
Cac8I	GCNNGC	4 cut(s)	79, 83, 801, 1206
CaiI	CAGNNNCTG	2 cut(s)	448, 1084
CfoI	GCGC	1 cut(s)	1383
Cfr10I	RCCGGY	1 cut(s)	257
Cfr13I	GGNCC	4 cut(s)	36, 445, 647, 1431
CsiI	ACCWGGT	1 cut(s)	1433
Csp6I	GTAC	4 cut(s)	463, 506, 824, 847
CviQI	GTAC	4 cut(s)	463, 506, 824, 847
DdeI	CTNAG	1 cut(s)	809
DpnI	GATC	5 cut(s)	288, 397, 433, 996, 1053
DpnII	GATC	5 cut(s)	286, 395, 431, 994, 1051
DraI	TTTAAA	1 cut(s)	940
EaeI	YGGCCR	1 cut(s)	403
Eam1104I	CTCTTC	3 cut(s)	593, 1164, 1403
EarI	CTCTTC	3 cut(s)	593, 1164, 1403
EciI	GGCGGA	1 cut(s)	1139
Ecl136II	GAGCTC	1 cut(s)	976
Eco130I	CCWWGG	1 cut(s)	346
Eco147I	AGGCCT	1 cut(s)	105
Eco24I	GRGCYC	1 cut(s)	978
Eco31I	GGTCTC	1 cut(s)	1232
Eco47I	GGWCC	4 cut(s)	36, 445, 647, 1431
Eco53kI	GAGCTC	1 cut(s)	976
Eco57I	CTGAAG	2 cut(s)	617, 1188
Eco91I	GGTNACC	1 cut(s)	437
EcoICRI	GAGCTC	1 cut(s)	976
EcoO109I	RGGNCCY	2 cut(s)	36, 445
EcoO65I	GGTNACC	1 cut(s)	437
EcoRI	GAATTC	1 cut(s)	668
EcoRII	CCWGG	4 cut(s)	441, 447, 551, 1433
EcoT14I	CCWWGG	1 cut(s)	346
EcoT38I	GRGCYC	1 cut(s)	978
ErhI	CCWWGG	1 cut(s)	346
FaqI	GGGAC	1 cut(s)	527
FauNDI	CATATG	1 cut(s)	637
Fnu4HI	GCNGC	5 cut(s)	232, 235, 352, 693, 954
FokI	GGATG	5 cut(s)	17, 722, 1022, 1081, 1139
FriOI	GRGCYC	1 cut(s)	978
Fsp4HI	GCNGC	5 cut(s)	232, 235, 352, 693, 954
FspBI	CTAG	6 cut(s)	78, 170, 888, 1152, 1215, 1226
GlaI	GCGC	1 cut(s)	1382
GluI	GCNGC	5 cut(s)	232, 235, 352, 693, 954
GsuI	CTGGAG	1 cut(s)	1220
HaeII	RGCGCY	1 cut(s)	1384
HaeIII	GGCC	4 cut(s)	105, 405, 788, 840
HapII	CCGG	1 cut(s)	258
HhaI	GCGC	1 cut(s)	1383
Hin6I	GCGC	1 cut(s)	1381
HinP1I	GCGC	1 cut(s)	1381
HinfI	GANTC	8 cut(s)	522, 592, 618, 1081, 1218, 1274, 1287, 1355
HpaII	CCGG	1 cut(s)	258
HphI	GGTGA	3 cut(s)	341, 449, 574
Hpy166II	GTNNAC	2 cut(s)	463, 1021
Hpy188III	TCNNGA	5 cut(s)	22, 170, 1215, 1284, 1327
Hpy8I	GTNNAC	2 cut(s)	463, 1021
Hpy99I	CGWCG	1 cut(s)	141
HpyAV	CCTTC	9 cut(s)	27, 143, 176, 673, 700, 734, 778, 830, 1300
HpyCH4III	ACNGT	3 cut(s)	47, 539, 1025
HpyCH4IV	ACGT	1 cut(s)	822
HpyF10VI	GCNNNNNNNGC	4 cut(s)	263, 316, 689, 1136
HpyF3I	CTNAG	1 cut(s)	809
HpySE526I	ACGT	1 cut(s)	822
HspAI	GCGC	1 cut(s)	1381
Kzo9I	GATC	5 cut(s)	286, 395, 431, 994, 1051
LmnI	GCTCC	5 cut(s)	53, 950, 973, 1201, 1234
Lsp1109I	GCAGC	5 cut(s)	243, 246, 338, 704, 965
LweI	GCATC	4 cut(s)	361, 614, 700, 1117
MabI	ACCWGGT	1 cut(s)	1433
MaeI	CTAG	6 cut(s)	78, 170, 888, 1152, 1215, 1226
MaeII	ACGT	1 cut(s)	822
MaeIII	GTNAC	2 cut(s)	419, 437
MalI	GATC	5 cut(s)	288, 397, 433, 996, 1053
MboI	GATC	5 cut(s)	286, 395, 431, 994, 1051
MboII	GAAGA	8 cut(s)	610, 827, 1123, 1181, 1265, 1342, 1360, 1420
MflI	RGATCY	3 cut(s)	395, 431, 1051
MhlI	GDGCHC	2 cut(s)	546, 978
MlsI	TGGCCA	1 cut(s)	405
MluCI	AATT	5 cut(s)	668, 1073, 1105, 1243, 1370
MluNI	TGGCCA	1 cut(s)	405
MlyI	GAGTC	2 cut(s)	1075, 1227
MmeI	TCCRAC	4 cut(s)	153, 231, 1246, 1398
Mox20I	TGGCCA	1 cut(s)	405
MscI	TGGCCA	1 cut(s)	405
MseI	TTAA	3 cut(s)	246, 486, 939
MslI	CAYNNNNRTG	1 cut(s)	495
Msp20I	TGGCCA	1 cut(s)	405
MspA1I	CMGCKG	1 cut(s)	956
MspI	CCGG	1 cut(s)	258
MspR9I	CCNGG	4 cut(s)	443, 449, 553, 1435
Mva1269I	GAATGC	1 cut(s)	777
MvaI	CCWGG	4 cut(s)	443, 449, 553, 1435
MvnI	CGCG	1 cut(s)	327
MwoI	GCNNNNNNNGC	4 cut(s)	263, 316, 689, 1136
NcoI	CCATGG	1 cut(s)	346
NdeI	CATATG	1 cut(s)	637
NdeII	GATC	5 cut(s)	286, 395, 431, 994, 1051
NheI	GCTAGC	1 cut(s)	77
NlaIV	GGNNCC	2 cut(s)	38, 1422
NmuCI	GTSAC	1 cut(s)	437
NspI	RCATGY	2 cut(s)	205, 803
OliI	CACNNNNGTG	1 cut(s)	495
PaeI	GCATGC	1 cut(s)	803
PceI	AGGCCT	1 cut(s)	105
PciI	ACATGT	1 cut(s)	201
PctI	GAATGC	1 cut(s)	777
PfeI	GAWTC	6 cut(s)	522, 592, 618, 1274, 1287, 1355
PflMI	CCANNNNNTGG	1 cut(s)	448
PkrI	GCNGC	5 cut(s)	233, 236, 353, 694, 955
PleI	GAGTC	2 cut(s)	1075, 1226
PpsI	GAGTC	2 cut(s)	1075, 1226
PpuMI	RGGWCCY	2 cut(s)	36, 445
PscI	ACATGT	1 cut(s)	201
Psp124BI	GAGCTC	1 cut(s)	978
Psp5II	RGGWCCY	2 cut(s)	36, 445
Psp6I	CCWGG	4 cut(s)	441, 447, 551, 1433
PspEI	GGTNACC	1 cut(s)	437
PspGI	CCWGG	4 cut(s)	441, 447, 551, 1433
PspN4I	GGNNCC	2 cut(s)	38, 1422
PspPI	GGNCC	4 cut(s)	36, 445, 647, 1431
PspPPI	RGGWCCY	2 cut(s)	36, 445
PstI	CTGCAG	1 cut(s)	1065
PstNI	CAGNNNCTG	2 cut(s)	448, 1084
PsuI	RGATCY	3 cut(s)	395, 431, 1051
PvuII	CAGCTG	1 cut(s)	956
RsaI	GTAC	4 cut(s)	464, 507, 825, 848
RsaNI	GTAC	4 cut(s)	463, 506, 824, 847
RseI	CAYNNNNRTG	1 cut(s)	495
SacI	GAGCTC	1 cut(s)	978
SaqAI	TTAA	3 cut(s)	246, 486, 939
SatI	GCNGC	5 cut(s)	232, 235, 352, 693, 954
Sau3AI	GATC	5 cut(s)	286, 395, 431, 994, 1051
Sau96I	GGNCC	4 cut(s)	36, 445, 647, 1431
SchI	GAGTC	2 cut(s)	1075, 1227
ScrFI	CCNGG	4 cut(s)	443, 449, 553, 1435
SduI	GDGCHC	2 cut(s)	546, 978
SexAI	ACCWGGT	1 cut(s)	1433
SfaNI	GCATC	4 cut(s)	361, 614, 700, 1117
SfcI	CTRYAG	1 cut(s)	1061
SinI	GGWCC	4 cut(s)	36, 445, 647, 1431
SmiMI	CAYNNNNRTG	1 cut(s)	495
SmlI	CTYRAG	4 cut(s)	20, 864, 1235, 1261
SmoI	CTYRAG	4 cut(s)	20, 864, 1235, 1261
SpeI	ACTAGT	1 cut(s)	1151
SphI	GCATGC	1 cut(s)	803
Sse9I	AATT	5 cut(s)	668, 1073, 1105, 1243, 1370
SseBI	AGGCCT	1 cut(s)	105
SsiI	CCGC	3 cut(s)	310, 327, 1124
SspI	AATATT	1 cut(s)	1322
SspMI	CTAG	6 cut(s)	78, 170, 888, 1152, 1215, 1226
SstI	GAGCTC	1 cut(s)	978
StuI	AGGCCT	1 cut(s)	105
StyD4I	CCNGG	4 cut(s)	441, 447, 551, 1433
StyI	CCWWGG	1 cut(s)	346
TaaI	ACNGT	3 cut(s)	47, 539, 1025
TaiI	ACGT	1 cut(s)	825
TasI	AATT	5 cut(s)	668, 1073, 1105, 1243, 1370
TatI	WGTACW	3 cut(s)	462, 505, 846
TfiI	GAWTC	6 cut(s)	522, 592, 618, 1274, 1287, 1355
Tru1I	TTAA	3 cut(s)	246, 486, 939
Tru9I	TTAA	3 cut(s)	246, 486, 939
TscAI	CASTG	3 cut(s)	544, 1171, 1203
TseFI	GTSAC	1 cut(s)	437
TseI	GCWGC	5 cut(s)	231, 234, 351, 692, 953
Tsp45I	GTSAC	1 cut(s)	437
TspDTI	ATGAA	5 cut(s)	546, 661, 1085, 1266, 1383
TspRI	CASTG	3 cut(s)	544, 1171, 1203
Van91I	CCANNNNNTGG	1 cut(s)	448
VpaK11BI	GGWCC	4 cut(s)	36, 445, 647, 1431
XapI	RAATTY	2 cut(s)	668, 1105
XbaI	TCTAGA	2 cut(s)	169, 1214
XceI	RCATGY	2 cut(s)	205, 803
XspI	CTAG	6 cut(s)	78, 170, 888, 1152, 1215, 1226

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.