Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001382

Physical Location & Seq

Genomic Coordinates Forward (+)

25140110 .. 25144006

Gene Structure 3897 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1157300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1668 bp

ATGGCCCCGGTTGAAGACATAGCAGAGAAAGAGAAAGCAGCTCCCGAAGCAAAACCAGAAGCAGAGGTGGAAGAAGAGGAAGTGGAAGAAGACGAAGAGGAGGAGGAGCTTTGGACGTCGGACTCCGACGTCGGAGATGCGTTGGACTATTTGGACTCCAAGGACGACGACAGAGGAGTAGAAGGCGGCTTTACCATCAATTCACGGCGGCCCAATGCCCACGGCGGCCTTCACTCCCGCCCTGCTTCCTCGACCCTTCAGCCTCTCGCTAATCGCAACCAGAAGTTCACTACCCACATCCGAGCTTCGCCTCTGGAGGAGTGGGAAGGGAGGTTCAACGTTGGAATGTCCAACTCCGTGACCACTGCCATTCGGGGAAGTGTTCGGGACATGGCCATTGGCAGAACGAAAACTACTGAGAAAGCAGACCGTGCCACTGTTGAGCAGGCCATCGATCCTAGAACTCGGATGGTTTTGTTCAAAATGTTGAATAGTGGCGTGTTTCATGATATCAATGGCTGCATTTCTACCGGGAAAGAAGCAAATGTGTATCACGCAACAAAATCTGATGGCCAGGAAATGGCTATCAAAGTGTTCAAAACATCTATTCTGGTTTTTAAGGACAGGGATAGATATGTACAAGGGGACTACCGATTCAGACATGGATACTGCAAGCGCAATCCTAGGAAAATGGTGCAGACATGGGCTGAGAAAGAAATGAGGAATTTAAAGAGGCTAAAGGAAAACGGAATTAGGTGCCCATCTACAGTTATTTTAAGACTTCATGTTCTGGTCATGGAATTCATAGGGAAATCGGGTTGGGCAGCACCTCGTCTTAAGGATGCTGCGTTATCGCTAGACAAGCTACGGGAAGGTTATATGGAGATAATTATTGCTATGCGAACAATGTATCAGAAGAGCAAATTGGTGCACGGAGACCTTAGTGAATACAACATACTTTACTTTGAGGGCCACTTGTACATTATTGATGTTTCTCAAGCAGTTGATCTGGACCACCCTCATGCCCTTGATTTCCTACGAGAAGATTGTGTCCATGTATCTGATTTCTTTAAGAAACATGGTGTAGCTGTTATGACAATTCGAGAGCTCTTTGATTTTATAGTTGATCCAACTATTGCTGATGATGCAGTGGAGAGTTATCTCGATATGGTACAACAAAAGGTTACGGCAAGAGGAGATATATCTGCAGAGGAGGAAATTGCAGACTCTGTATTTGTACAGTCGTACATTCCAAAGACTTTAGATGACGTGAAGAATGCTGAGCGGGATGTAATACGGTTAACTAGCGGAGAGGACACAGGGGACATGTACTATAAAACTATTACAGGACTGAAGGAGGCTCTTCCTCATGGTCAACCAGCTTCATCTGAGAAGGAGCAGAAACAAGATGATGCTACTGCTGTAGCAGATCCCTCTGAAAATCCCGAGACTGAATCTGACTCTGATACAGATGACAACTCAAGTGACTCGGAAGGGCAGAGCTCCTCTTCCGAAACCAAGGCACAGGATCCTCTCGAAAAGAAAGCTGCTAGGAAAGAGAACAAGAAGAAAGTGAAGGAAGAGAAGAGGGAAGCTCGGAAAACCAAAGTGCCGAAGGCTCTGAAGAAACGAAAGAAAAAGATGGCCAAAGCACACAAGACCAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0016070 GO:0016072 GO:0019219 GO:0019222 GO:0022613 GO:0030490 GO:0030684 GO:0030688 GO:0031323 GO:0031325 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0034708 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090069 GO:0090070 GO:0090304 GO:1901360 GO:1902494 GO:1990234 GO:1990904 GO:2000232 GO:2000234

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

RIO1

Protein Analysis

556

Amino Acids

62.68

Weight (kDa)

5.62

Isoelectric Point (pI)

41.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RIO1	PF01163	182 - 369	4.6e-72	RIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24990 AT5G37350 AT5G37350
fragaria_vesca	FvH4_5g08310 FvH4_5g08310 FvH4_5g08310 FvH4_5g16770 FvH4_5g16770 FvH4_5g16770 FvH4_5g25261
malus_domestica	MD06G1142000.v1.1 MD14G1157300.v1.1
prunus_persica	Prupe.5G151200_v2.0.a1
pyrus_communis	pycom06g13180 pycom14g13080
rosa_chinensis	RchiOBHm_Chr5g0020401 RchiOBHm_Chr5g0063241 RchiOBHm_Chr5g0063291 RchiOBHm_Chr7g0190211
rosa_laevigata	RLG00000004553 RLG00000032520 RLG00000035614 RLG00000035619
rosa_multiflora	Rmu_sc0000033.1_g000014 Rmu_sc0004886.1_g000019 Rmu_sc0005045.1_g000016
rosa_roxburghii	Rroxscaffold_1G00017410 Rroxscaffold_1G00017420 Rroxscaffold_1G00017470 Rroxscaffold_3G00264780
rosa_rugosa	Rorug05G0058900 Rorug05G0058900 Rorug05G0059000 Rorug05G0059100 Rorug05G0355500 Rorug06G0503000
rosa_samantha	Rh5AG149600 Rh5AG415200 Rh5AG415300 Rh5AG415500 Rh5BG149000 Rh5BG430400 Rh5CG160500 Rh5CG453800 Rh5DG147800 Rh5DG444000 Rh7AG108300 Rh7BG110900 Rh7CG113300 Rh7DG111700
rosa_wichuraiana	Rw5G013250 Rw5G039050 Rw7G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	128
AatII	GACGTC	2 cut(s)	119, 132
AccB1I	GGYRCC	1 cut(s)	756
AccB7I	CCANNNNNTGG	1 cut(s)	580
AccBSI	CCGCTC	1 cut(s)	1285
AciI	CCGC	6 cut(s)	186, 208, 225, 238, 1285, 1308
AclI	AACGTT	1 cut(s)	339
AclWI	GGATC	5 cut(s)	449, 1121, 1424, 1523, 1536
AcoI	YGGCCR	3 cut(s)	393, 571, 1644
AcsI	RAATTY	2 cut(s)	724, 800
AcuI	CTGAAG	3 cut(s)	242, 1373, 1643
AcyI	GRCGYC	2 cut(s)	116, 129
AfaI	GTAC	6 cut(s)	639, 980, 1173, 1239, 1247, 1331
AfiI	CCNNNNNNNGG	1 cut(s)	580
AflII	CTTAAG	1 cut(s)	836
AflIII	ACRYGT	1 cut(s)	1326
AgsI	TTSAA	5 cut(s)	14, 337, 481, 490, 598
AjiI	CACGTC	1 cut(s)	1270
AjnI	CCWGG	2 cut(s)	573, 1661
AjuI	GAANNNNNNNTTGG	2 cut(s)	908, 940
AloI	GAACNNNNNNTCC	2 cut(s)	317, 349
Alw21I	GWGCWC	3 cut(s)	933, 1110, 1505
Alw26I	GTCTC	2 cut(s)	930, 1442
Alw44I	GTGCAC	1 cut(s)	929
AlwI	GGATC	5 cut(s)	449, 1121, 1424, 1523, 1536
AlwNI	CAGNNNCTG	1 cut(s)	1229
Ama87I	CYCGRG	1 cut(s)	1445
AoxI	GGCC	8 cut(s)	3, 209, 226, 393, 447, 571, 970, 1644
ApaLI	GTGCAC	1 cut(s)	929
ApeKI	GCWGC	5 cut(s)	38, 519, 824, 845, 1547
ApoI	RAATTY	2 cut(s)	724, 800
ArsI	GACNNNNNNTTYG	2 cut(s)	1217, 1249
AspA2I	CCTAGG	1 cut(s)	683
AspLEI	GCGC	1 cut(s)	678
AspS9I	GGNCC	4 cut(s)	4, 210, 970, 1012
AsuC2I	CCSGG	2 cut(s)	8, 532
AvaI	CYCGRG	1 cut(s)	1445
AvaII	GGWCC	1 cut(s)	1012
AvrII	CCTAGG	1 cut(s)	683
BaeGI	GKGCMC	2 cut(s)	761, 933
BalI	TGGCCA	3 cut(s)	395, 573, 1646
BamHI	GGATCC	1 cut(s)	1528
BanI	GGYRCC	1 cut(s)	756
BanII	GRGCYC	2 cut(s)	1110, 1505
BbsI	GAAGAC	2 cut(s)	21, 96
Bbv12I	GWGCWC	3 cut(s)	933, 1110, 1505
BbvI	GCAGC	5 cut(s)	50, 506, 832, 836, 1534
BccI	CCATC	6 cut(s)	203, 458, 463, 563, 769, 1636
BceAI	ACGGC	3 cut(s)	221, 238, 1203
BciT130I	CCWGG	2 cut(s)	575, 1663
BciVI	GTATCC	1 cut(s)	659
BcnI	CCSGG	2 cut(s)	8, 532
BcoDI	GTCTC	2 cut(s)	930, 1442
BfaI	CTAG	5 cut(s)	459, 684, 857, 1305, 1551
BfmI	CTRYAG	3 cut(s)	765, 1206, 1422
BfrI	CTTAAG	1 cut(s)	836
BfuI	GTATCC	1 cut(s)	659
BisI	GCNGC	8 cut(s)	39, 187, 209, 226, 520, 825, 846, 1548
BlnI	CCTAGG	1 cut(s)	683
BlpI	GCTNAGC	1 cut(s)	1281
BlsI	GCNGC	8 cut(s)	40, 188, 210, 227, 521, 826, 847, 1549
Bme1390I	CCNGG	4 cut(s)	8, 532, 575, 1663
Bme18I	GGWCC	1 cut(s)	1012
BmeT110I	CYCGRG	1 cut(s)	1445
BmgBI	CACGTC	1 cut(s)	1270
BmgT120I	GGNCC	4 cut(s)	4, 210, 970, 1012
BmiI	GGNNCC	3 cut(s)	6, 758, 1530
BmrFI	CCNGG	4 cut(s)	8, 532, 575, 1663
BmsI	GCATC	4 cut(s)	127, 832, 1135, 1402
BpiI	GAAGAC	2 cut(s)	21, 96
BplI	GAGNNNNNCTC	2 cut(s)	1579, 1611
BpmI	CTGGAG	1 cut(s)	335
Bpu1102I	GCTNAGC	1 cut(s)	1281
BpuEI	CTTGAG	2 cut(s)	981, 1465
BpuMI	CCSGG	2 cut(s)	8, 532
Bsa29I	ATCGAT	1 cut(s)	453
BsaHI	GRCGYC	2 cut(s)	116, 129
BsaI	GGTCTC	1 cut(s)	930
BsaJI	CCNNGG	5 cut(s)	6, 159, 220, 683, 1518
Bsc4I	CCNNNNNNNGG	1 cut(s)	580
BseBI	CCWGG	2 cut(s)	575, 1663
BseCI	ATCGAT	1 cut(s)	453
BseDI	CCNNGG	5 cut(s)	6, 159, 220, 683, 1518
BseGI	GGATG	4 cut(s)	297, 474, 847, 1294
BseLI	CCNNNNNNNGG	1 cut(s)	580
BseMII	CTCAG	4 cut(s)	408, 699, 1272, 1380
BseRI	GAGGAG	8 cut(s)	113, 116, 119, 189, 332, 1209, 1226, 1495
BseSI	GKGCMC	2 cut(s)	761, 933
BseXI	GCAGC	5 cut(s)	50, 506, 832, 836, 1534
BsgI	GTGCAG	1 cut(s)	716
BshFI	GGCC	8 cut(s)	5, 211, 228, 395, 449, 573, 972, 1646
BshNI	GGYRCC	1 cut(s)	756
BshVI	ATCGAT	1 cut(s)	453
BsiHKAI	GWGCWC	3 cut(s)	933, 1110, 1505
BsiHKCI	CYCGRG	1 cut(s)	1445
BsiSI	CCGG	2 cut(s)	8, 531
BslFI	GGGAC	3 cut(s)	401, 659, 1337
BslI	CCNNNNNNNGG	1 cut(s)	580
BsmAI	GTCTC	2 cut(s)	930, 1442
BsmFI	GGGAC	3 cut(s)	401, 659, 1337
BsmI	GAATGC	1 cut(s)	1282
BsnI	GGCC	8 cut(s)	5, 211, 228, 395, 449, 573, 972, 1646
Bso31I	GGTCTC	1 cut(s)	930
BsoBI	CYCGRG	1 cut(s)	1445
Bsp1286I	GDGCHC	4 cut(s)	761, 933, 1110, 1505
Bsp1407I	TGTACA	3 cut(s)	637, 978, 1237
Bsp143I	GATC	5 cut(s)	454, 1006, 1126, 1429, 1528
Bsp1720I	GCTNAGC	1 cut(s)	1281
BspACI	CCGC	6 cut(s)	186, 208, 225, 238, 1285, 1308
BspANI	GGCC	8 cut(s)	5, 211, 228, 395, 449, 573, 972, 1646
BspCNI	CTCAG	4 cut(s)	409, 700, 1273, 1381
BspDI	ATCGAT	1 cut(s)	453
BspHI	TCATGA	1 cut(s)	505
BspLI	GGNNCC	3 cut(s)	6, 758, 1530
BspMAI	CTGCAG	1 cut(s)	1210
BspPI	GGATC	5 cut(s)	449, 1121, 1424, 1523, 1536
BspQI	GCTCTTC	2 cut(s)	911, 1368
BspT107I	GGYRCC	1 cut(s)	756
BspTI	CTTAAG	1 cut(s)	836
BspTNI	GGTCTC	1 cut(s)	930
BsrBI	CCGCTC	1 cut(s)	1285
BsrGI	TGTACA	3 cut(s)	637, 978, 1237
BssECI	CCNNGG	5 cut(s)	6, 159, 220, 683, 1518
BssMI	GATC	5 cut(s)	454, 1006, 1126, 1429, 1528
BssNI	GRCGYC	2 cut(s)	116, 129
BssT1I	CCWWGG	3 cut(s)	159, 683, 1518
Bst2UI	CCWGG	2 cut(s)	575, 1663
Bst4CI	ACNGT	5 cut(s)	431, 439, 769, 1242, 1299
Bst6I	CTCTTC	7 cut(s)	69, 90, 911, 1368, 1513, 1575, 1580
BstACI	GRCGYC	2 cut(s)	116, 129
BstAFI	CTTAAG	1 cut(s)	836
BstAPI	GCANNNNNTGC	1 cut(s)	431
BstAUI	TGTACA	3 cut(s)	637, 978, 1237
BstC8I	GCNNGC	2 cut(s)	447, 674
BstDEI	CTNAG	5 cut(s)	417, 708, 941, 1281, 1389
BstDSI	CCRYGG	1 cut(s)	220
BstF5I	GGATG	4 cut(s)	297, 474, 847, 1294
BstHHI	GCGC	1 cut(s)	678
BstKTI	GATC	5 cut(s)	457, 1009, 1129, 1432, 1531
BstMAI	GTCTC	2 cut(s)	930, 1442
BstMBI	GATC	5 cut(s)	454, 1006, 1126, 1429, 1528
BstMWI	GCNNNNNNNGC	4 cut(s)	47, 431, 862, 1145
BstNI	CCWGG	2 cut(s)	575, 1663
BstNSI	RCATGY	1 cut(s)	1330
BstSCI	CCNGG	4 cut(s)	6, 530, 573, 1661
BstSFI	CTRYAG	3 cut(s)	765, 1206, 1422
BstSLI	GKGCMC	2 cut(s)	761, 933
BstV1I	GCAGC	5 cut(s)	50, 506, 832, 836, 1534
BstV2I	GAAGAC	2 cut(s)	21, 96
BstX2I	RGATCY	2 cut(s)	1429, 1528
BstYI	RGATCY	2 cut(s)	1429, 1528
Bsu15I	ATCGAT	1 cut(s)	453
BsuI	GTATCC	1 cut(s)	659
BsuRI	GGCC	8 cut(s)	5, 211, 228, 395, 449, 573, 972, 1646
BsuTUI	ATCGAT	1 cut(s)	453
BtgI	CCRYGG	1 cut(s)	220
BtrI	CACGTC	1 cut(s)	1270
BtsCI	GGATG	4 cut(s)	297, 474, 847, 1294
BtsI	GCAGTG	2 cut(s)	363, 1155
BtsIMutI	CAGTG	3 cut(s)	363, 435, 1155
Cac8I	GCNNGC	2 cut(s)	447, 674
CaiI	CAGNNNCTG	1 cut(s)	1229
CciI	TCATGA	1 cut(s)	505
CfoI	GCGC	1 cut(s)	678
Cfr13I	GGNCC	4 cut(s)	4, 210, 970, 1012
ClaI	ATCGAT	1 cut(s)	453
CsiI	ACCWGGT	1 cut(s)	1661
Csp6I	GTAC	6 cut(s)	638, 979, 1172, 1238, 1246, 1330
CviQI	GTAC	6 cut(s)	638, 979, 1172, 1238, 1246, 1330
DdeI	CTNAG	5 cut(s)	417, 708, 941, 1281, 1389
DpnI	GATC	5 cut(s)	456, 1008, 1128, 1431, 1530
DpnII	GATC	5 cut(s)	454, 1006, 1126, 1429, 1528
DraI	TTTAAA	1 cut(s)	729
DrdI	GACNNNNNNGTC	1 cut(s)	128
DseDI	GACNNNNNNGTC	1 cut(s)	128
EaeI	YGGCCR	3 cut(s)	393, 571, 1644
Eam1104I	CTCTTC	7 cut(s)	69, 90, 911, 1368, 1513, 1575, 1580
EarI	CTCTTC	7 cut(s)	69, 90, 911, 1368, 1513, 1575, 1580
Ecl136II	GAGCTC	2 cut(s)	1108, 1503
Eco130I	CCWWGG	3 cut(s)	159, 683, 1518
Eco24I	GRGCYC	2 cut(s)	1110, 1505
Eco31I	GGTCTC	1 cut(s)	930
Eco32I	GATATC	1 cut(s)	511
Eco47I	GGWCC	1 cut(s)	1012
Eco53kI	GAGCTC	2 cut(s)	1108, 1503
Eco57I	CTGAAG	3 cut(s)	242, 1373, 1643
Eco88I	CYCGRG	1 cut(s)	1445
EcoICRI	GAGCTC	2 cut(s)	1108, 1503
EcoRI	GAATTC	1 cut(s)	800
EcoRII	CCWGG	2 cut(s)	573, 1661
EcoRV	GATATC	1 cut(s)	511
EcoT14I	CCWWGG	3 cut(s)	159, 683, 1518
EcoT38I	GRGCYC	2 cut(s)	1110, 1505
ErhI	CCWWGG	3 cut(s)	159, 683, 1518
FaqI	GGGAC	3 cut(s)	401, 659, 1337
FauI	CCCGC	2 cut(s)	245, 1278
Fnu4HI	GCNGC	8 cut(s)	39, 187, 209, 226, 520, 825, 846, 1548
FokI	GGATG	4 cut(s)	284, 481, 854, 1301
FriOI	GRGCYC	2 cut(s)	1110, 1505
Fsp4HI	GCNGC	8 cut(s)	39, 187, 209, 226, 520, 825, 846, 1548
FspBI	CTAG	5 cut(s)	459, 684, 857, 1305, 1551
GlaI	GCGC	1 cut(s)	677
GluI	GCNGC	8 cut(s)	39, 187, 209, 226, 520, 825, 846, 1548
GsuI	CTGGAG	1 cut(s)	335
HaeIII	GGCC	8 cut(s)	5, 211, 228, 395, 449, 573, 972, 1646
HapII	CCGG	2 cut(s)	8, 531
HhaI	GCGC	1 cut(s)	678
Hin1I	GRCGYC	2 cut(s)	116, 129
Hin6I	GCGC	1 cut(s)	676
HinP1I	GCGC	1 cut(s)	676
HincII	GTYRAC	2 cut(s)	1302, 1376
HindII	GTYRAC	2 cut(s)	1302, 1376
HinfI	GANTC	7 cut(s)	122, 155, 654, 1226, 1454, 1460, 1487
HpaI	GTTAAC	1 cut(s)	1302
HpaII	CCGG	2 cut(s)	8, 531
Hpy166II	GTNNAC	4 cut(s)	288, 931, 1302, 1376
Hpy188III	TCNNGA	9 cut(s)	44, 314, 386, 506, 1010, 1103, 1163, 1445, 1535
Hpy8I	GTNNAC	4 cut(s)	288, 931, 1302, 1376
Hpy99I	CGWCG	4 cut(s)	121, 131, 134, 170
HpyCH4III	ACNGT	5 cut(s)	431, 439, 769, 1242, 1299
HpyCH4IV	ACGT	4 cut(s)	116, 129, 339, 1269
HpyCH4V	TGCA	7 cut(s)	522, 672, 697, 931, 1148, 1208, 1223
HpyF10VI	GCNNNNNNNGC	4 cut(s)	47, 431, 862, 1145
HpyF3I	CTNAG	5 cut(s)	417, 708, 941, 1281, 1389
HpySE526I	ACGT	4 cut(s)	116, 129, 339, 1269
Hsp92I	GRCGYC	2 cut(s)	116, 129
HspAI	GCGC	1 cut(s)	676
KspAI	GTTAAC	1 cut(s)	1302
Kzo9I	GATC	5 cut(s)	454, 1006, 1126, 1429, 1528
LguI	GCTCTTC	2 cut(s)	911, 1368
LmnI	GCTCC	4 cut(s)	46, 106, 1396, 1508
Lsp1109I	GCAGC	5 cut(s)	50, 506, 832, 836, 1534
LweI	GCATC	4 cut(s)	127, 832, 1135, 1402
MabI	ACCWGGT	1 cut(s)	1661
MaeI	CTAG	5 cut(s)	459, 684, 857, 1305, 1551
MaeII	ACGT	4 cut(s)	116, 129, 339, 1269
MaeIII	GTNAC	3 cut(s)	358, 1183, 1484
MalI	GATC	5 cut(s)	456, 1008, 1128, 1431, 1530
MbiI	CCGCTC	1 cut(s)	1285
MboI	GATC	5 cut(s)	454, 1006, 1126, 1429, 1528
MflI	RGATCY	2 cut(s)	1429, 1528
MhlI	GDGCHC	4 cut(s)	761, 933, 1110, 1505
MlsI	TGGCCA	3 cut(s)	395, 573, 1646
MluCI	AATT	8 cut(s)	199, 724, 750, 800, 888, 923, 1098, 1218
MluNI	TGGCCA	3 cut(s)	395, 573, 1646
MlyI	GAGTC	5 cut(s)	116, 149, 1220, 1454, 1481
MmeI	TCCRAC	7 cut(s)	99, 112, 123, 150, 322, 375, 1154
Mox20I	TGGCCA	3 cut(s)	395, 573, 1646
MscI	TGGCCA	3 cut(s)	395, 573, 1646
MseI	TTAA	6 cut(s)	618, 728, 776, 837, 1071, 1301
MslI	CAYNNNNRTG	1 cut(s)	1020
Msp20I	TGGCCA	3 cut(s)	395, 573, 1646
MspCI	CTTAAG	1 cut(s)	836
MspI	CCGG	2 cut(s)	8, 531
MspR9I	CCNGG	4 cut(s)	8, 532, 575, 1663
Mva1269I	GAATGC	1 cut(s)	1282
MvaI	CCWGG	2 cut(s)	575, 1663
MwoI	GCNNNNNNNGC	4 cut(s)	47, 431, 862, 1145
NciI	CCSGG	2 cut(s)	8, 532
NdeII	GATC	5 cut(s)	454, 1006, 1126, 1429, 1528
NlaIV	GGNNCC	3 cut(s)	6, 758, 1530
NmuCI	GTSAC	2 cut(s)	358, 1484
NspI	RCATGY	1 cut(s)	1330
PagI	TCATGA	1 cut(s)	505
PciI	ACATGT	1 cut(s)	1326
PciSI	GCTCTTC	2 cut(s)	911, 1368
PctI	GAATGC	1 cut(s)	1282
PfeI	GAWTC	2 cut(s)	654, 1454
PflMI	CCANNNNNTGG	1 cut(s)	580
PkrI	GCNGC	8 cut(s)	40, 188, 210, 227, 521, 826, 847, 1549
PleI	GAGTC	5 cut(s)	116, 149, 1220, 1454, 1481
PpsI	GAGTC	5 cut(s)	116, 149, 1220, 1454, 1481
PscI	ACATGT	1 cut(s)	1326
Psp124BI	GAGCTC	2 cut(s)	1110, 1505
Psp1406I	AACGTT	1 cut(s)	339
Psp6I	CCWGG	2 cut(s)	573, 1661
PspGI	CCWGG	2 cut(s)	573, 1661
PspN4I	GGNNCC	3 cut(s)	6, 758, 1530
PspPI	GGNCC	4 cut(s)	4, 210, 970, 1012
PsrI	GAACNNNNNNTAC	2 cut(s)	397, 429
PstI	CTGCAG	1 cut(s)	1210
PstNI	CAGNNNCTG	1 cut(s)	1229
PsuI	RGATCY	2 cut(s)	1429, 1528
RsaI	GTAC	6 cut(s)	639, 980, 1173, 1239, 1247, 1331
RsaNI	GTAC	6 cut(s)	638, 979, 1172, 1238, 1246, 1330
RseI	CAYNNNNRTG	1 cut(s)	1020
SacI	GAGCTC	2 cut(s)	1110, 1505
SapI	GCTCTTC	2 cut(s)	911, 1368
SaqAI	TTAA	6 cut(s)	618, 728, 776, 837, 1071, 1301
SatI	GCNGC	8 cut(s)	39, 187, 209, 226, 520, 825, 846, 1548
Sau3AI	GATC	5 cut(s)	454, 1006, 1126, 1429, 1528
Sau96I	GGNCC	4 cut(s)	4, 210, 970, 1012
SchI	GAGTC	5 cut(s)	116, 149, 1220, 1454, 1481
ScrFI	CCNGG	4 cut(s)	8, 532, 575, 1663
SduI	GDGCHC	4 cut(s)	761, 933, 1110, 1505
SexAI	ACCWGGT	1 cut(s)	1661
SfaNI	GCATC	4 cut(s)	127, 832, 1135, 1402
SfcI	CTRYAG	3 cut(s)	765, 1206, 1422
SinI	GGWCC	1 cut(s)	1012
SmiMI	CAYNNNNRTG	1 cut(s)	1020
SmlI	CTYRAG	3 cut(s)	836, 996, 1480
SmoI	CTYRAG	3 cut(s)	836, 996, 1480
Sse9I	AATT	8 cut(s)	199, 724, 750, 800, 888, 923, 1098, 1218
SsiI	CCGC	6 cut(s)	186, 208, 225, 238, 1285, 1308
SspMI	CTAG	5 cut(s)	459, 684, 857, 1305, 1551
SstI	GAGCTC	2 cut(s)	1110, 1505
StyD4I	CCNGG	4 cut(s)	6, 530, 573, 1661
StyI	CCWWGG	3 cut(s)	159, 683, 1518
TaaI	ACNGT	5 cut(s)	431, 439, 769, 1242, 1299
TaiI	ACGT	4 cut(s)	119, 132, 342, 1272
TaqI	TCGA	5 cut(s)	251, 453, 1102, 1164, 1536
TasI	AATT	8 cut(s)	199, 724, 750, 800, 888, 923, 1098, 1218
TatI	WGTACW	4 cut(s)	637, 978, 1237, 1329
TauI	GCSGC	3 cut(s)	189, 211, 228
TfiI	GAWTC	2 cut(s)	654, 1454
Tru1I	TTAA	6 cut(s)	618, 728, 776, 837, 1071, 1301
Tru9I	TTAA	6 cut(s)	618, 728, 776, 837, 1071, 1301
TscAI	CASTG	3 cut(s)	370, 442, 1155
TseFI	GTSAC	2 cut(s)	358, 1484
TseI	GCWGC	5 cut(s)	38, 519, 824, 845, 1547
Tsp45I	GTSAC	2 cut(s)	358, 1484
TspDTI	ATGAA	4 cut(s)	494, 773, 793, 1374
TspGWI	ACGGA	3 cut(s)	346, 762, 948
TspRI	CASTG	3 cut(s)	370, 442, 1155
Van91I	CCANNNNNTGG	1 cut(s)	580
Vha464I	CTTAAG	1 cut(s)	836
VneI	GTGCAC	1 cut(s)	929
VpaK11BI	GGWCC	1 cut(s)	1012
XapI	RAATTY	2 cut(s)	724, 800
XceI	RCATGY	1 cut(s)	1330
XmaJI	CCTAGG	1 cut(s)	683
XspI	CTAG	5 cut(s)	459, 684, 857, 1305, 1551
ZraI	GACGTC	2 cut(s)	117, 130

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.