Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0001382

Physical Location & Seq

Genomic Coordinates Forward (+)

8816573 .. 8820255

Gene Structure 3683 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G009390.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1212 bp

ATGGCTGCCGTTGAAGAGACAAAATCAGAGAAAGAGACTGAAGTGATTACTCCTGAAGCCGAAGAAGAAGAAGAAGTAGTAGTAGTCGAAGAGGATGAGGAAGAGGACTGGTCATCGGACTCGGACATCGGAGATGCATTGGACTATTTGGATTCCAAGGACGACGACGCAGTTGAAGGCGCCTTCACTATCAATTCGCGGCGCCCAAATGCCCATGGGGGCCTTCACTCTCACCCTAACTCCTCTACTCTTCAGCCTCTCTCTAATCGCAACCAGAGGTTCTCCACTCACATCCGAGCTTCGCCGTTAGAGGAGTGGGAAGGGAGGCATGTTGGGATGTCCAACTCTGTGACGACTGCGATTAGGGAAAGTGTTCGGGACATGGCCACTGGAAGAACAAAGACTACTGAGAAGGCGGATCGTGCCACTGTTGAGCAGGCTATTGATCCTAGAACTCGGATGGTGTTATTCAAAATGCTGAATCGCGGAGTGTTTAAGGATATCAATGGCTGCATTTCTACTGGGAAAGAAGCAAATGTTTATCATGCAACAAAAGCTGATGGCGGGGAACTGGCAATCAAAGTCTTCAAAACCTCTATTCTTGTTTTTAAGGATAGGGATAGATATGTACAAGGGGACTACAGGTTCAGACATGGATACTGCAAGCACAATCCTAGGAAAATGGTGAAGACATGGGCCGAGAAAGAAAAAAGGAATTTGATCAGGCTAAAGGCTGAAGGAATTAGGTGCCCAGAAGTAGGTCCTCTAAGACTTCATGTTCTGGTGATGGAATTCATAGGTAAGGATGGTTGGGCAGCGCCACGCCTAAAGGATGCTGCATTATCACTTGACAAGCTACGAGAAGGTTATATGGAGATGGTTATTGCTATGCGAACATTATATCAGAAGTGCAAACTGGTGCATGGTGATCTCAGTGAATACAACATACTTTATTTTGAGGGCCACTTGTATATCATCGATGTTTCTCAATCAGTTGATCTCGACCACCCTCATGCTCTTGATTTCCTACGTGAAGATTGTGTCCATGTATCCGATTTCTTCAAAAAACATGGTGTAGCTGTCATGACAATTCGAGAGCTCTTTGACTTTATAGTGGATCCATGTATTGCTGATGATGATATAGACAGTTATCTGGAGAAGGTTTGGTTCCTTTTGCTCTCTATTATGAATTCTTGGGCTCCTATTAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0016070 GO:0016072 GO:0019219 GO:0019222 GO:0022613 GO:0030490 GO:0030684 GO:0030688 GO:0031323 GO:0031325 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0034708 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090069 GO:0090070 GO:0090304 GO:1901360 GO:1902494 GO:1990234 GO:1990904 GO:2000232 GO:2000234

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

RIO1

Protein Analysis

403

Amino Acids

45.97

Weight (kDa)

5.34

Isoelectric Point (pI)

31.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RIO1	PF01163	179 - 366	1.7e-70	RIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24990 AT5G37350 AT5G37350
fragaria_vesca	FvH4_5g08310 FvH4_5g08310 FvH4_5g08310 FvH4_5g16770 FvH4_5g16770 FvH4_5g16770 FvH4_5g25261
malus_domestica	MD06G1142000.v1.1 MD14G1157300.v1.1
prunus_persica	Prupe.5G151200_v2.0.a1
pyrus_communis	pycom06g13180 pycom14g13080
rosa_chinensis	RchiOBHm_Chr5g0020401 RchiOBHm_Chr5g0063241 RchiOBHm_Chr5g0063291 RchiOBHm_Chr7g0190211
rosa_laevigata	RLG00000004553 RLG00000032520 RLG00000035614 RLG00000035619
rosa_multiflora	Rmu_sc0000033.1_g000014 Rmu_sc0004886.1_g000019 Rmu_sc0005045.1_g000016
rosa_roxburghii	Rroxscaffold_1G00017410 Rroxscaffold_1G00017420 Rroxscaffold_1G00017470 Rroxscaffold_3G00264780
rosa_rugosa	Rorug05G0058900 Rorug05G0058900 Rorug05G0059000 Rorug05G0059100 Rorug05G0355500 Rorug06G0503000
rosa_samantha	Rh5AG149600 Rh5AG415200 Rh5AG415300 Rh5AG415500 Rh5BG149000 Rh5BG430400 Rh5CG160500 Rh5CG453800 Rh5DG147800 Rh5DG444000 Rh7AG108300 Rh7BG110900 Rh7CG113300 Rh7DG111700
rosa_wichuraiana	Rw5G013250 Rw5G039050 Rw7G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	179, 201, 747
AccII	CGCG	2 cut(s)	199, 486
AciI	CCGC	4 cut(s)	199, 416, 486, 564
AclWI	GGATC	4 cut(s)	426, 440, 1112, 1125
AcoI	YGGCCR	1 cut(s)	384
AcsI	RAATTY	3 cut(s)	715, 791, 1189
AcuI	CTGAAG	4 cut(s)	60, 75, 236, 756
AcyI	GRCGYC	2 cut(s)	180, 202
AfaI	GTAC	1 cut(s)	630
AfiI	CCNNNNNNNGG	1 cut(s)	758
AgsI	TTSAA	5 cut(s)	14, 176, 472, 589, 1063
AloI	GAACNNNNNNTCC	2 cut(s)	629, 661
AluBI	AGCT	5 cut(s)	299, 557, 856, 1079, 1099
AluI	AGCT	5 cut(s)	299, 557, 856, 1079, 1099
Alw21I	GWGCWC	1 cut(s)	1101
Alw26I	GTCTC	2 cut(s)	11, 29
AlwI	GGATC	4 cut(s)	426, 440, 1112, 1125
AoxI	GGCC	4 cut(s)	220, 384, 696, 961
ApeKI	GCWGC	4 cut(s)	5, 510, 815, 836
ApoI	RAATTY	3 cut(s)	715, 791, 1189
Asp700I	GAANNNNTTC	1 cut(s)	372
AspA2I	CCTAGG	1 cut(s)	674
AspLEI	GCGC	3 cut(s)	182, 204, 820
AspS9I	GGNCC	4 cut(s)	220, 696, 761, 961
AsuHPI	GGTGA	4 cut(s)	224, 697, 796, 938
AvaII	GGWCC	1 cut(s)	761
AvrII	CCTAGG	1 cut(s)	674
BaeGI	GKGCMC	1 cut(s)	752
BalI	TGGCCA	1 cut(s)	386
BamHI	GGATCC	1 cut(s)	1117
BanI	GGYRCC	3 cut(s)	179, 201, 747
BanII	GRGCYC	2 cut(s)	1101, 1201
BbsI	GAAGAC	2 cut(s)	577, 695
Bbv12I	GWGCWC	1 cut(s)	1101
BbvI	GCAGC	3 cut(s)	497, 823, 827
BccI	CCATC	5 cut(s)	454, 554, 781, 800, 871
BceAI	ACGGC	1 cut(s)	289
BciVI	GTATCC	2 cut(s)	650, 1060
BclI	TGATCA	1 cut(s)	720
BcoDI	GTCTC	2 cut(s)	11, 29
BfaI	CTAG	2 cut(s)	450, 675
BfmI	CTRYAG	1 cut(s)	640
BfoI	RGCGCY	3 cut(s)	183, 205, 821
BfuI	GTATCC	2 cut(s)	650, 1060
BisI	GCNGC	5 cut(s)	6, 200, 511, 816, 837
BlnI	CCTAGG	1 cut(s)	674
BlsI	GCNGC	5 cut(s)	7, 201, 512, 817, 838
Bme18I	GGWCC	1 cut(s)	761
BmgT120I	GGNCC	4 cut(s)	220, 696, 761, 961
BmiI	GGNNCC	7 cut(s)	181, 203, 221, 749, 1119, 1169, 1200
BmrI	ACTGGG	1 cut(s)	531
BmsI	GCATC	2 cut(s)	124, 823
BmuI	ACTGGG	1 cut(s)	531
BpiI	GAAGAC	2 cut(s)	577, 695
BpmI	CTGGAG	1 cut(s)	1175
Bsa29I	ATCGAT	1 cut(s)	978
BsaAI	YACGTR	1 cut(s)	1031
BsaHI	GRCGYC	2 cut(s)	180, 202
BsaJI	CCNNGG	3 cut(s)	156, 214, 674
BsaXI	ACNNNNNCTCC	2 cut(s)	224, 254
Bsc4I	CCNNNNNNNGG	1 cut(s)	758
Bse1I	ACTGG	5 cut(s)	113, 394, 526, 576, 921
BseCI	ATCGAT	1 cut(s)	978
BseDI	CCNNGG	3 cut(s)	156, 214, 674
BseGI	GGATG	6 cut(s)	100, 291, 342, 465, 811, 838
BseLI	CCNNNNNNNGG	1 cut(s)	758
BseMII	CTCAG	2 cut(s)	399, 946
BseNI	ACTGG	5 cut(s)	113, 394, 526, 576, 921
BseRI	GAGGAG	2 cut(s)	232, 326
BseSI	GKGCMC	1 cut(s)	752
BseXI	GCAGC	3 cut(s)	497, 823, 827
Bsh1236I	CGCG	2 cut(s)	199, 486
BshFI	GGCC	4 cut(s)	222, 386, 698, 963
BshNI	GGYRCC	3 cut(s)	179, 201, 747
BshVI	ATCGAT	1 cut(s)	978
BsiHKAI	GWGCWC	1 cut(s)	1101
BslFI	GGGAC	2 cut(s)	392, 650
BslI	CCNNNNNNNGG	1 cut(s)	758
BsmAI	GTCTC	2 cut(s)	11, 29
BsmFI	GGGAC	2 cut(s)	392, 650
BsnI	GGCC	4 cut(s)	222, 386, 698, 963
Bsp1286I	GDGCHC	3 cut(s)	752, 1101, 1201
Bsp1407I	TGTACA	1 cut(s)	628
Bsp143I	GATC	6 cut(s)	418, 445, 720, 928, 997, 1117
Bsp19I	CCATGG	1 cut(s)	214
BspACI	CCGC	4 cut(s)	199, 416, 486, 564
BspANI	GGCC	4 cut(s)	222, 386, 698, 963
BspCNI	CTCAG	2 cut(s)	400, 945
BspDI	ATCGAT	1 cut(s)	978
BspFNI	CGCG	2 cut(s)	199, 486
BspHI	TCATGA	1 cut(s)	1083
BspLI	GGNNCC	7 cut(s)	181, 203, 221, 749, 1119, 1169, 1200
BspPI	GGATC	4 cut(s)	426, 440, 1112, 1125
BspT107I	GGYRCC	3 cut(s)	179, 201, 747
BsrGI	TGTACA	1 cut(s)	628
BsrI	ACTGG	5 cut(s)	113, 394, 526, 576, 921
BssECI	CCNNGG	3 cut(s)	156, 214, 674
BssMI	GATC	6 cut(s)	418, 445, 720, 928, 997, 1117
BssNI	GRCGYC	2 cut(s)	180, 202
BssT1I	CCWWGG	3 cut(s)	156, 214, 674
Bst4CI	ACNGT	2 cut(s)	430, 1148
Bst6I	CTCTTC	4 cut(s)	9, 84, 96, 255
BstACI	GRCGYC	2 cut(s)	180, 202
BstAUI	TGTACA	1 cut(s)	628
BstBAI	YACGTR	1 cut(s)	1031
BstC8I	GCNNGC	2 cut(s)	438, 665
BstDEI	CTNAG	3 cut(s)	408, 767, 932
BstDSI	CCRYGG	1 cut(s)	214
BstF5I	GGATG	6 cut(s)	100, 291, 342, 465, 811, 838
BstFNI	CGCG	2 cut(s)	199, 486
BstH2I	RGCGCY	3 cut(s)	183, 205, 821
BstHHI	GCGC	3 cut(s)	182, 204, 820
BstKTI	GATC	6 cut(s)	421, 448, 723, 931, 1000, 1120
BstMAI	GTCTC	2 cut(s)	11, 29
BstMBI	GATC	6 cut(s)	418, 445, 720, 928, 997, 1117
BstMWI	GCNNNNNNNGC	2 cut(s)	422, 554
BstNSI	RCATGY	1 cut(s)	332
BstSFI	CTRYAG	1 cut(s)	640
BstSLI	GKGCMC	1 cut(s)	752
BstUI	CGCG	2 cut(s)	199, 486
BstV1I	GCAGC	3 cut(s)	497, 823, 827
BstV2I	GAAGAC	2 cut(s)	577, 695
BstX2I	RGATCY	1 cut(s)	1117
BstYI	RGATCY	1 cut(s)	1117
Bsu15I	ATCGAT	1 cut(s)	978
BsuI	GTATCC	2 cut(s)	650, 1060
BsuRI	GGCC	4 cut(s)	222, 386, 698, 963
BsuTUI	ATCGAT	1 cut(s)	978
BtgI	CCRYGG	1 cut(s)	214
BtsCI	GGATG	6 cut(s)	100, 291, 342, 465, 811, 838
BtsIMutI	CAGTG	3 cut(s)	387, 426, 940
Cac8I	GCNNGC	2 cut(s)	438, 665
CciI	TCATGA	1 cut(s)	1083
CfoI	GCGC	3 cut(s)	182, 204, 820
Cfr13I	GGNCC	4 cut(s)	220, 696, 761, 961
ClaI	ATCGAT	1 cut(s)	978
CseI	GACGC	1 cut(s)	176
Csp6I	GTAC	1 cut(s)	629
CviQI	GTAC	1 cut(s)	629
DdeI	CTNAG	3 cut(s)	408, 767, 932
DinI	GGCGCC	2 cut(s)	181, 203
DpnI	GATC	6 cut(s)	420, 447, 722, 930, 999, 1119
DpnII	GATC	6 cut(s)	418, 445, 720, 928, 997, 1117
EaeI	YGGCCR	1 cut(s)	384
Eam1104I	CTCTTC	4 cut(s)	9, 84, 96, 255
EarI	CTCTTC	4 cut(s)	9, 84, 96, 255
EciI	GGCGGA	1 cut(s)	431
Ecl136II	GAGCTC	1 cut(s)	1099
Eco130I	CCWWGG	3 cut(s)	156, 214, 674
Eco24I	GRGCYC	2 cut(s)	1101, 1201
Eco32I	GATATC	1 cut(s)	502
Eco47I	GGWCC	1 cut(s)	761
Eco53kI	GAGCTC	1 cut(s)	1099
Eco57I	CTGAAG	4 cut(s)	60, 75, 236, 756
EcoICRI	GAGCTC	1 cut(s)	1099
EcoO109I	RGGNCCY	2 cut(s)	220, 761
EcoRI	GAATTC	2 cut(s)	791, 1189
EcoRV	GATATC	1 cut(s)	502
EcoT14I	CCWWGG	3 cut(s)	156, 214, 674
EcoT22I	ATGCAT	1 cut(s)	139
EcoT38I	GRGCYC	2 cut(s)	1101, 1201
EgeI	GGCGCC	2 cut(s)	181, 203
EheI	GGCGCC	2 cut(s)	181, 203
ErhI	CCWWGG	3 cut(s)	156, 214, 674
FaqI	GGGAC	2 cut(s)	392, 650
FauI	CCCGC	1 cut(s)	557
FbaI	TGATCA	1 cut(s)	720
Fnu4HI	GCNGC	5 cut(s)	6, 200, 511, 816, 837
FokI	GGATG	6 cut(s)	107, 278, 349, 472, 818, 845
FriOI	GRGCYC	2 cut(s)	1101, 1201
Fsp4HI	GCNGC	5 cut(s)	6, 200, 511, 816, 837
FspBI	CTAG	2 cut(s)	450, 675
GlaI	GCGC	3 cut(s)	181, 203, 819
GluI	GCNGC	5 cut(s)	6, 200, 511, 816, 837
GsuI	CTGGAG	1 cut(s)	1175
HaeII	RGCGCY	3 cut(s)	183, 205, 821
HaeIII	GGCC	4 cut(s)	222, 386, 698, 963
HgaI	GACGC	1 cut(s)	176
HhaI	GCGC	3 cut(s)	182, 204, 820
Hin1I	GRCGYC	2 cut(s)	180, 202
Hin6I	GCGC	3 cut(s)	180, 202, 818
HinP1I	GCGC	3 cut(s)	180, 202, 818
HinfI	GANTC	3 cut(s)	119, 152, 481
HphI	GGTGA	4 cut(s)	224, 697, 796, 938
Hpy188I	TCNGA	9 cut(s)	28, 118, 124, 131, 296, 459, 650, 906, 1054
Hpy188III	TCNNGA	7 cut(s)	53, 377, 1001, 1019, 1084, 1094, 1154
Hpy99I	CGWCG	2 cut(s)	167, 170
HpyAV	CCTTC	8 cut(s)	170, 193, 233, 314, 406, 731, 857, 1153
HpyCH4III	ACNGT	2 cut(s)	430, 1148
HpyCH4IV	ACGT	1 cut(s)	1030
HpyCH4V	TGCA	7 cut(s)	137, 513, 548, 663, 839, 912, 922
HpyF10VI	GCNNNNNNNGC	2 cut(s)	422, 554
HpyF3I	CTNAG	3 cut(s)	408, 767, 932
HpySE526I	ACGT	1 cut(s)	1030
Hsp92I	GRCGYC	2 cut(s)	180, 202
HspAI	GCGC	3 cut(s)	180, 202, 818
KasI	GGCGCC	2 cut(s)	179, 201
Ksp22I	TGATCA	1 cut(s)	720
Kzo9I	GATC	6 cut(s)	418, 445, 720, 928, 997, 1117
LmnI	GCTCC	1 cut(s)	1204
Lsp1109I	GCAGC	3 cut(s)	497, 823, 827
LweI	GCATC	2 cut(s)	124, 823
MaeI	CTAG	2 cut(s)	450, 675
MaeII	ACGT	1 cut(s)	1030
MaeIII	GTNAC	1 cut(s)	349
MalI	GATC	6 cut(s)	420, 447, 722, 930, 999, 1119
MboI	GATC	6 cut(s)	418, 445, 720, 928, 997, 1117
MflI	RGATCY	1 cut(s)	1117
MhlI	GDGCHC	3 cut(s)	752, 1101, 1201
MlsI	TGGCCA	1 cut(s)	386
MluCI	AATT	6 cut(s)	193, 715, 741, 791, 1089, 1189
MluNI	TGGCCA	1 cut(s)	386
Mly113I	GGCGCC	2 cut(s)	180, 202
MlyI	GAGTC	1 cut(s)	113
MmeI	TCCRAC	1 cut(s)	366
Mox20I	TGGCCA	1 cut(s)	386
Mph1103I	ATGCAT	1 cut(s)	139
MroXI	GAANNNNTTC	1 cut(s)	372
MscI	TGGCCA	1 cut(s)	386
MseI	TTAA	2 cut(s)	495, 609
MslI	CAYNNNNRTG	1 cut(s)	1011
Msp20I	TGGCCA	1 cut(s)	386
MvnI	CGCG	2 cut(s)	199, 486
MwoI	GCNNNNNNNGC	2 cut(s)	422, 554
NarI	GGCGCC	2 cut(s)	180, 202
NcoI	CCATGG	1 cut(s)	214
NdeII	GATC	6 cut(s)	418, 445, 720, 928, 997, 1117
NlaIV	GGNNCC	7 cut(s)	181, 203, 221, 749, 1119, 1169, 1200
NmeAIII	GCCGAG	1 cut(s)	724
NmuCI	GTSAC	1 cut(s)	349
NsiI	ATGCAT	1 cut(s)	139
NspI	RCATGY	1 cut(s)	332
PagI	TCATGA	1 cut(s)	1083
PdmI	GAANNNNTTC	1 cut(s)	372
PfeI	GAWTC	2 cut(s)	152, 481
PkrI	GCNGC	5 cut(s)	7, 201, 512, 817, 838
PleI	GAGTC	1 cut(s)	113
PluTI	GGCGCC	2 cut(s)	183, 205
PpsI	GAGTC	1 cut(s)	113
Ppu21I	YACGTR	1 cut(s)	1031
PpuMI	RGGWCCY	1 cut(s)	761
Psp124BI	GAGCTC	1 cut(s)	1101
Psp5II	RGGWCCY	1 cut(s)	761
PspN4I	GGNNCC	7 cut(s)	181, 203, 221, 749, 1119, 1169, 1200
PspPI	GGNCC	4 cut(s)	220, 696, 761, 961
PspPPI	RGGWCCY	1 cut(s)	761
PsrI	GAACNNNNNNTAC	2 cut(s)	388, 420
PsuI	RGATCY	1 cut(s)	1117
RsaI	GTAC	1 cut(s)	630
RsaNI	GTAC	1 cut(s)	629
RseI	CAYNNNNRTG	1 cut(s)	1011
SacI	GAGCTC	1 cut(s)	1101
SaqAI	TTAA	2 cut(s)	495, 609
SatI	GCNGC	5 cut(s)	6, 200, 511, 816, 837
Sau3AI	GATC	6 cut(s)	418, 445, 720, 928, 997, 1117
Sau96I	GGNCC	4 cut(s)	220, 696, 761, 961
SchI	GAGTC	1 cut(s)	113
SduI	GDGCHC	3 cut(s)	752, 1101, 1201
SfaNI	GCATC	2 cut(s)	124, 823
SfcI	CTRYAG	1 cut(s)	640
SfoI	GGCGCC	2 cut(s)	181, 203
SinI	GGWCC	1 cut(s)	761
SmiMI	CAYNNNNRTG	1 cut(s)	1011
Sse9I	AATT	6 cut(s)	193, 715, 741, 791, 1089, 1189
SsiI	CCGC	4 cut(s)	199, 416, 486, 564
SspDI	GGCGCC	2 cut(s)	179, 201
SspMI	CTAG	2 cut(s)	450, 675
SstI	GAGCTC	1 cut(s)	1101
StyI	CCWWGG	3 cut(s)	156, 214, 674
TaaI	ACNGT	2 cut(s)	430, 1148
TaiI	ACGT	1 cut(s)	1033
TaqI	TCGA	4 cut(s)	87, 978, 1002, 1093
TasI	AATT	6 cut(s)	193, 715, 741, 791, 1089, 1189
TatI	WGTACW	1 cut(s)	628
TauI	GCSGC	1 cut(s)	202
TfiI	GAWTC	2 cut(s)	152, 481
Tru1I	TTAA	2 cut(s)	495, 609
Tru9I	TTAA	2 cut(s)	495, 609
TscAI	CASTG	3 cut(s)	394, 433, 940
TseFI	GTSAC	1 cut(s)	349
TseI	GCWGC	4 cut(s)	5, 510, 815, 836
Tsp45I	GTSAC	1 cut(s)	349
TspDTI	ATGAA	3 cut(s)	764, 784, 1202
TspRI	CASTG	3 cut(s)	394, 433, 940
VpaK11BI	GGWCC	1 cut(s)	761
XapI	RAATTY	3 cut(s)	715, 791, 1189
XceI	RCATGY	1 cut(s)	332
XmaJI	CCTAGG	1 cut(s)	674
XmnI	GAANNNNTTC	1 cut(s)	372
XspI	CTAG	2 cut(s)	450, 675
Zsp2I	ATGCAT	1 cut(s)	139

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.