Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002460

Physical Location & Seq

Genomic Coordinates Reverse (-)

31477223 .. 31482198

Gene Structure 4976 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1250100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2628 bp

ATGGACGATTATCAGGAGATGGAGAGGTTTGGAATGGAGAAGGATTACGAGGACGGCCAGTGGATCGGCGGGGAGTTCTATTACCGCAAGCGCAAGGACAAGCGCGTCCAAACCAAAGACGACGTCCTCTACGGCATATTCACTGCCGATTCCGACGACGACGATGACAACGACGGCCCCAGAAAACGCAGGAAAGACCGAAAGGTCGACCTCACCAAGCCCGTCAGTTTCATCTCCACCGGAATCGTCATGCCCAACCAGGAGGTCGACGACAATTCAAGGCAGCAAAACGATGATAACCGGCCTGGCCTCGGCGCCACCATTGGCCTCGGCTTTGGTGCCGCTACCGGTTCCGGTTTAGGTTTTAAAATTCCGAATTTGGATCCGTTGGGGGGCGAGGAGGATGACGACGACGAGCAGAGTTTTTTGCCGACAGCGTTTGGGAAGAAGATAAAGGAGGGAGCGGAGAGGAGACACAAGGAGAGGGAAAGGCTGAAATTGCTCAAGCAGACTAGTAGTCAGTCTCAGGGTCGAGGTTCTGAAGAAGGCCAATTTGCAGGATTAGGAGCAAAGGGCGGCGGGGATGGGGGTTTGGGGGCCTTCGAGAAACACACCAAGGGCATTGGAATGAAGATGCTTAAGAACATGGGCTACAAAGGAGGCGGACTTGGGAAGAACGAGCAAGGCATTCTCGCTCCTATTGAAGCCAAGTTGAGGCCAAAGAATATGGGCATGGGTTTCAATGATTACAAGGAGACGGAAGTCAAGCGGCCTAGCTTGCAAGAATTGGAAGCTGAAAAGCCGAGCAAGCCTTTGCCGGATGCGACCTCCGCCAAAAAGAAGCCTTCCTGGAAGAAGAGGGCCGCTGCCAACAAGACTGAGAAGGACCAGTATGTCTCTGCCAAGGAGTTGTTAGCCCAGAAGCAGGAAGAGGGCACCGATGTCCTTGTGCACAAGGTGGTTGATATGCGAGGACCTCAGGTTCGAGTCTTGACCAATCTGGAGAATTTGAATGCCGAAGAGAAAGCCAGGGAAGAGGATGTTCCCATGCCCGAGCTACAGCACAACGTCAGGTTGATTCTGGACTTAGCTGAGCTTGATATCCAGAAGATTGACAGGGACCTGAGGATTGAGCGGGACACGGCCATCAGCTTGAACCAGGAGAAAGAGAGGTTGGAGACGGAGGTGGCCAGGCAGAAGCAACACCTGGATAGCTTGGAGGATATTAGGACCGTCTTGGATCGACTGGGAGAAGAGAATAGCATGGGAACATTGACGCTGGACTCCCTGACAAAGGGCTTTGGTGACCTGCAGAAGAGATACGCTGATGACTATAAGTTATGTAACCTGTCTTGCATTGCCTGCTCCTTTGCTCTGCCTCTGTTTATTAGGATGTTTCAGGGCTGGAATCCTCTTAGGAACCCCTCGCATGGGTTGGATTTAGTATCTTCTTGGAAGGCTTTGCTTCATGGAGAGGGGGAAAGAGAGCAGTACTTGGATATTTGGGATAACTCAATGTCCCCTTACACCCAATTGCTTTCCGAGGTTGTAGTACCTGCCATTAGGATTGCTGGCGTGAATACATGGCAGCCCAAGGACCCTGAACCCATGCTTCGCTTTTTGGAGTCCTGGGAGAAATTGCTGCCTCCTTCTGTCCTTCATTCCATATTGGATATGGTAATCTTTCCTAAATTGAAGGACGCAGTTGACTTGTGGGAACCTCACCGGGACACTGTTCCCATCCATGTGTGGGTGCATCCGTGGCTACCATTCTTGGGACACAAGTTGGAAGACTTGTATCACACAATACGATTCAAGTTGAGTAATGTTCTGGGTGCTTGGCACCCAAGTGATGGATCTGCCTATACTATACTCTCACCGTGGAAGAAGGTGTTTGATGCTGCGAGTTGGGAACAGCTTATGTATAGATTTATAGTACCCAAGTTGCAGCTTGTCTTACAAGAATTCCAAGTGAACCCTGCAGATCAGACGCTTGATCAATATAATTGGGTGATGAGTTGGGCTTCTGCTATTCCAATTCATCTAATGGTAGATATGATGGAGAAATTTTTCTTCACCAAGTGGCTACAGGTTTTGTATCACTGGTTATGTTCAAACCCGAACTTTGAAGAGGTTTTAAATTGGTATAAAGGTTGGAAGGAACTCATTCCAGAGGAGCTTCATGCTAATGAAAGTATCCGGTATCAGCTTAATTGTGGTCTTGACATGATGAATCGGGCTGTTGAGGGTATGGAGGTGGTCCAACCTGGTTTGAAGGAGAATATTAGCTACTTTAGGGTGCTTGAGCAAAGGCAATTTGAGGCACATCAGAAAGCAGCAGCAGCAGCACAGGCAAACTTGGGTGGTACTGCTCAAATGGATGGCATTGGTCATGAGATGAATTTGAAAGATGTTATTGAGGCCCATGCGCAGCAGCATGGCCTGCTATTTAGGCCTAAGCCTGGGAGGATGCATAATGGTCATCAGATGTATGGCTTTGGTAATGTAAGCATAATAGTTGACTCTCTAAATCAAAAGGTATATGCCCAAACAGAGGAAACTTGGTCCCTTGTATCCCTTGAAAGGTTGCTGGACATGCACAAAAATTCACTTAAAAGGAGACGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000375 GO:0000377 GO:0000390 GO:0000398 GO:0000781 GO:0000784 GO:0003674 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005694 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0022411 GO:0030154 GO:0030198 GO:0031012 GO:0031333 GO:0031974 GO:0031981 GO:0032091 GO:0032502 GO:0032984 GO:0032988 GO:0032991 GO:0034641 GO:0042752 GO:0043021 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043393 GO:0043933 GO:0044087 GO:0044092 GO:0044237 GO:0044238 GO:0044421 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0046483 GO:0048519 GO:0048523 GO:0048869 GO:0050789 GO:0050794 GO:0051098 GO:0051100 GO:0051128 GO:0051129 GO:0065007 GO:0065009 GO:0070013 GO:0070990 GO:0071008 GO:0071013 GO:0071014 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0098687 GO:1901360 GO:1902494 GO:1990446 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-patch GCFC TIP_N

Protein Analysis

876

Amino Acids

99.92

Weight (kDa)

5.82

Isoelectric Point (pI)

41.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIP_N	PF12457	2 - 100	1.1e-20	Tuftelin interacting protein N terminal
G-patch	PF01585	205 - 246	5e-14	G-patch domain
GCFC	PF07842	427 - 699	9.5e-91	GC-rich sequence DNA-binding factor-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002460)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17070
fragaria_vesca	FvH4_2g25582 FvH4_5g27320
malus_domestica	MD08G1250100.v1.1 MD15G1445100.v1.1
prunus_persica	Prupe.1G587200_v2.0.a1 Prupe.5G048700_v2.0.a1
pyrus_communis	pycom08g21680 pycom15g39220
rosa_chinensis	RchiOBHm_Chr6g0293801 RchiOBHm_Chr6g0293811 RchiOBHm_Chr7g0218481
rosa_laevigata	RLG00000002370 RLG00000011921 RLG00000012034
rosa_multiflora	Rmu_co8485319.1_g000001 Rmu_sc0001301.1_g000062 Rmu_sc0001301.1_g000063 Rmu_sc0001354.1_g000013 Rmu_sc0023058.1_g000001
rosa_roxburghii	Rroxscaffold_3G00240910 Rroxscaffold_7G00173890 Rroxscaffold_7G00175120
rosa_rugosa	Rorug06G0238200 Rorug07G0177400
rosa_samantha	Rh6AG339400 Rh6AG351600 Rh6BG346700 Rh6BG358400 Rh6CG353500 Rh6CG365600 Rh6DG339500 Rh6DG352200 Rh6DG352300 Rh7AG318900 Rh7BG310800 Rh7CG335800 Rh7DG318000
rosa_wichuraiana	Rw6G029540 Rw6G030580 Rw7G027140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	104, 203, 893
AatII	GACGTC	1 cut(s)	126
Acc16I	TGCGCA	1 cut(s)	2430
Acc36I	ACCTGC	2 cut(s)	1317, 1564
AccB1I	GGYRCC	4 cut(s)	314, 338, 935, 1842
AccB7I	CCANNNNNTGG	1 cut(s)	1853
AccBSI	CCGCTC	2 cut(s)	464, 1135
AccI	GTMKAC	2 cut(s)	207, 267
AccII	CGCG	1 cut(s)	105
AclWI	GGATC	5 cut(s)	71, 377, 390, 1248, 1864
AcoI	YGGCCR	3 cut(s)	55, 1143, 1188
AcsI	RAATTY	7 cut(s)	369, 376, 1006, 1964, 2066, 2401, 2605
AcuI	CTGAAG	1 cut(s)	561
AcyI	GRCGYC	2 cut(s)	123, 315
AdeI	CACNNNGTG	2 cut(s)	958, 2082
AfaI	GTAC	4 cut(s)	1493, 1554, 1938, 2368
AflII	CTTAAG	1 cut(s)	638
AgeI	ACCGGT	1 cut(s)	347
AhdI	GACNNNNNGTC	1 cut(s)	516
AhlI	ACTAGT	1 cut(s)	512
AjuI	GAANNNNNNNTTGG	2 cut(s)	1157, 1189
AleI	CACNNNNGTG	1 cut(s)	1848
AloI	GAACNNNNNNTCC	2 cut(s)	966, 998
Alw21I	GWGCWC	1 cut(s)	954
Alw26I	GTCTC	6 cut(s)	466, 528, 749, 901, 1172, 2614
Alw44I	GTGCAC	1 cut(s)	950
AlwI	GGATC	5 cut(s)	71, 377, 390, 1248, 1864
Ama87I	CYCGRG	1 cut(s)	1052
ApaLI	GTGCAC	1 cut(s)	950
ApoI	RAATTY	7 cut(s)	369, 376, 1006, 1964, 2066, 2401, 2605
ArsI	GACNNNNNNTTYG	4 cut(s)	108, 140, 966, 998
AsiGI	ACCGGT	1 cut(s)	347
Asp700I	GAANNNNTTC	2 cut(s)	2069, 2166
AspLEI	GCGC	4 cut(s)	93, 105, 317, 2431
AsuC2I	CCSGG	1 cut(s)	1727
AsuHPI	GGTGA	6 cut(s)	205, 1316, 1715, 1869, 2023, 2068
AvaI	CYCGRG	1 cut(s)	1052
AvaII	GGWCC	7 cut(s)	886, 974, 1120, 1230, 1597, 2260, 2565
AxyI	CCTNAGG	2 cut(s)	978, 1124
BaeGI	GKGCMC	2 cut(s)	938, 954
BalI	TGGCCA	1 cut(s)	1190
BamHI	GGATCC	1 cut(s)	382
BanI	GGYRCC	4 cut(s)	314, 338, 935, 1842
BbsI	GAAGAC	1 cut(s)	1797
Bbv12I	GWGCWC	1 cut(s)	954
BccI	CCATC	7 cut(s)	13, 578, 1154, 1748, 1847, 2053, 2375
BceAI	ACGGC	4 cut(s)	70, 148, 190, 1158
BciVI	GTATCC	2 cut(s)	2207, 2584
BclI	TGATCA	1 cut(s)	1996
BcnI	CCSGG	1 cut(s)	1727
BcoDI	GTCTC	6 cut(s)	466, 528, 749, 901, 1172, 2614
BcuI	ACTAGT	1 cut(s)	512
BfaI	CTAG	2 cut(s)	513, 774
BfmI	CTRYAG	4 cut(s)	1058, 1310, 1980, 2087
BfoI	RGCGCY	1 cut(s)	318
BfrI	CTTAAG	1 cut(s)	638
BfuAI	ACCTGC	2 cut(s)	1317, 1564
BfuI	GTATCC	2 cut(s)	2207, 2584
BlpI	GCTNAGC	1 cut(s)	1092
BmcAI	AGTACT	1 cut(s)	1493
Bme18I	GGWCC	7 cut(s)	886, 974, 1120, 1230, 1597, 2260, 2565
BmeRI	GACNNNNNGTC	1 cut(s)	516
BmeT110I	CYCGRG	1 cut(s)	1052
BmrI	ACTGGG	1 cut(s)	1256
BmsI	GCATC	5 cut(s)	624, 811, 1765, 1888, 2460
BmuI	ACTGGG	1 cut(s)	1256
BoxI	GACNNNNGTC	1 cut(s)	761
BpiI	GAAGAC	1 cut(s)	1797
BpmI	CTGGAG	1 cut(s)	1022
Bpu10I	CCTNAGC	1 cut(s)	2457
Bpu1102I	GCTNAGC	1 cut(s)	1092
BpuEI	CTTGAG	2 cut(s)	488, 2324
BpuMI	CCSGG	1 cut(s)	1727
BsaHI	GRCGYC	2 cut(s)	123, 315
BsaWI	WCCGGW	4 cut(s)	239, 347, 353, 2199
Bse118I	RCCGGY	2 cut(s)	300, 347
Bse1I	ACTGG	4 cut(s)	58, 889, 1251, 2108
Bse21I	CCTNAGG	2 cut(s)	978, 1124
Bse3DI	GCAATG	1 cut(s)	1356
BseGI	GGATG	9 cut(s)	409, 589, 826, 1045, 1398, 1740, 1756, 2386, 2475
BseMI	GCAATG	1 cut(s)	1356
BseMII	CTCAG	5 cut(s)	539, 870, 992, 1083, 1115
BseNI	ACTGG	4 cut(s)	58, 889, 1251, 2108
BseRI	GAGGAG	3 cut(s)	413, 484, 2189
BseSI	GKGCMC	2 cut(s)	938, 954
Bsh1236I	CGCG	1 cut(s)	105
BshNI	GGYRCC	4 cut(s)	314, 338, 935, 1842
BshTI	ACCGGT	1 cut(s)	347
BsiHKAI	GWGCWC	1 cut(s)	954
BsiHKCI	CYCGRG	1 cut(s)	1052
BsiSI	CCGG	7 cut(s)	240, 301, 348, 354, 818, 1726, 2200
BslFI	GGGAC	6 cut(s)	1133, 1151, 1504, 1742, 1791, 2551
BsmAI	GTCTC	6 cut(s)	466, 528, 749, 901, 1172, 2614
BsmBI	CGTCTC	3 cut(s)	749, 1172, 2614
BsmFI	GGGAC	6 cut(s)	1133, 1151, 1504, 1742, 1791, 2551
BsmI	GAATGC	2 cut(s)	687, 1018
BsoBI	CYCGRG	1 cut(s)	1052
Bsp1286I	GDGCHC	2 cut(s)	938, 954
Bsp143I	GATC	6 cut(s)	63, 382, 1240, 1856, 1984, 1996
Bsp1720I	GCTNAGC	1 cut(s)	1092
BspCNI	CTCAG	5 cut(s)	538, 871, 991, 1084, 1116
BspFNI	CGCG	1 cut(s)	105
BspHI	TCATGA	1 cut(s)	2392
BspMAI	CTGCAG	2 cut(s)	1314, 1984
BspMI	ACCTGC	2 cut(s)	1317, 1564
BspPI	GGATC	5 cut(s)	71, 377, 390, 1248, 1864
BspT107I	GGYRCC	4 cut(s)	314, 338, 935, 1842
BspTI	CTTAAG	1 cut(s)	638
BsrBI	CCGCTC	2 cut(s)	464, 1135
BsrDI	GCAATG	1 cut(s)	1356
BsrFI	RCCGGY	2 cut(s)	300, 347
BsrI	ACTGG	4 cut(s)	58, 889, 1251, 2108
BssAI	RCCGGY	2 cut(s)	300, 347
BssMI	GATC	6 cut(s)	63, 382, 1240, 1856, 1984, 1996
BssNI	GRCGYC	2 cut(s)	123, 315
BssT1I	CCWWGG	3 cut(s)	615, 903, 1593
Bst4CI	ACNGT	3 cut(s)	1234, 1735, 1881
Bst6I	CTCTTC	7 cut(s)	851, 924, 1014, 1029, 1248, 1310, 2124
BstACI	GRCGYC	2 cut(s)	123, 315
BstAFI	CTTAAG	1 cut(s)	638
BstAPI	GCANNNNNTGC	2 cut(s)	1362, 2443
BstC8I	GCNNGC	6 cut(s)	89, 779, 809, 1363, 1573, 2444
BstDEI	CTNAG	8 cut(s)	525, 879, 978, 1087, 1092, 1124, 1415, 2457
BstDSI	CCRYGG	2 cut(s)	1760, 1880
BstEII	GGTNACC	1 cut(s)	1304
BstENI	CCTNNNNNAGG	1 cut(s)	1292
BstF5I	GGATG	9 cut(s)	409, 589, 826, 1045, 1398, 1740, 1756, 2386, 2475
BstFNI	CGCG	1 cut(s)	105
BstH2I	RGCGCY	1 cut(s)	318
BstHHI	GCGC	4 cut(s)	93, 105, 317, 2431
BstKTI	GATC	6 cut(s)	66, 385, 1243, 1859, 1987, 1999
BstMAI	GTCTC	6 cut(s)	466, 528, 749, 901, 1172, 2614
BstMBI	GATC	6 cut(s)	63, 382, 1240, 1856, 1984, 1996
BstNSI	RCATGY	1 cut(s)	2599
BstPAI	GACNNNNGTC	1 cut(s)	761
BstPI	GGTNACC	1 cut(s)	1304
BstSFI	CTRYAG	4 cut(s)	1058, 1310, 1980, 2087
BstSLI	GKGCMC	2 cut(s)	938, 954
BstUI	CGCG	1 cut(s)	105
BstV2I	GAAGAC	1 cut(s)	1797
BstX2I	RGATCY	2 cut(s)	382, 1856
BstYI	RGATCY	2 cut(s)	382, 1856
Bsu36I	CCTNAGG	2 cut(s)	978, 1124
BsuI	GTATCC	2 cut(s)	2207, 2584
BtgI	CCRYGG	2 cut(s)	1760, 1880
BtsCI	GGATG	9 cut(s)	409, 589, 826, 1045, 1398, 1740, 1756, 2386, 2475
BtsI	GCAGTG	1 cut(s)	141
BtsIMutI	CAGTG	4 cut(s)	65, 141, 1731, 2101
BveI	ACCTGC	2 cut(s)	1317, 1564
Cac8I	GCNNGC	6 cut(s)	89, 779, 809, 1363, 1573, 2444
CciI	TCATGA	1 cut(s)	2392
CfoI	GCGC	4 cut(s)	93, 105, 317, 2431
Cfr10I	RCCGGY	2 cut(s)	300, 347
CseI	GACGC	4 cut(s)	94, 1285, 1709, 1999
CsiI	ACCWGGT	1 cut(s)	2266
Csp6I	GTAC	4 cut(s)	1492, 1553, 1937, 2367
CspAI	ACCGGT	1 cut(s)	347
CviQI	GTAC	4 cut(s)	1492, 1553, 1937, 2367
DdeI	CTNAG	8 cut(s)	525, 879, 978, 1087, 1092, 1124, 1415, 2457
DinI	GGCGCC	1 cut(s)	316
DpnI	GATC	6 cut(s)	65, 384, 1242, 1858, 1986, 1998
DpnII	GATC	6 cut(s)	63, 382, 1240, 1856, 1984, 1996
DraI	TTTAAA	2 cut(s)	367, 2139
DraIII	CACNNNGTG	2 cut(s)	958, 2082
DrdI	GACNNNNNNGTC	3 cut(s)	104, 203, 893
DriI	GACNNNNNGTC	1 cut(s)	516
DseDI	GACNNNNNNGTC	3 cut(s)	104, 203, 893
EaeI	YGGCCR	3 cut(s)	55, 1143, 1188
Eam1104I	CTCTTC	7 cut(s)	851, 924, 1014, 1029, 1248, 1310, 2124
Eam1105I	GACNNNNNGTC	1 cut(s)	516
EarI	CTCTTC	7 cut(s)	851, 924, 1014, 1029, 1248, 1310, 2124
EciI	GGCGGA	2 cut(s)	678, 820
Eco130I	CCWWGG	3 cut(s)	615, 903, 1593
Eco147I	AGGCCT	1 cut(s)	2455
Eco32I	GATATC	1 cut(s)	1102
Eco47I	GGWCC	7 cut(s)	886, 974, 1120, 1230, 1597, 2260, 2565
Eco57I	CTGAAG	1 cut(s)	561
Eco81I	CCTNAGG	2 cut(s)	978, 1124
Eco88I	CYCGRG	1 cut(s)	1052
Eco91I	GGTNACC	1 cut(s)	1304
EcoNI	CCTNNNNNAGG	1 cut(s)	1292
EcoO109I	RGGNCCY	4 cut(s)	597, 974, 1120, 1597
EcoO65I	GGTNACC	1 cut(s)	1304
EcoRI	GAATTC	1 cut(s)	1964
EcoRV	GATATC	1 cut(s)	1102
EcoT14I	CCWWGG	3 cut(s)	615, 903, 1593
EcoT22I	ATGCAT	1 cut(s)	2475
EgeI	GGCGCC	1 cut(s)	316
EheI	GGCGCC	1 cut(s)	316
ErhI	CCWWGG	3 cut(s)	615, 903, 1593
Esp3I	CGTCTC	3 cut(s)	749, 1172, 2614
FaqI	GGGAC	6 cut(s)	1133, 1151, 1504, 1742, 1791, 2551
FauI	CCCGC	3 cut(s)	62, 572, 1128
FbaI	TGATCA	1 cut(s)	1996
FblI	GTMKAC	2 cut(s)	207, 267
FokI	GGATG	9 cut(s)	416, 596, 833, 1052, 1405, 1727, 1743, 2393, 2482
FspBI	CTAG	2 cut(s)	513, 774
FspI	TGCGCA	1 cut(s)	2430
GlaI	GCGC	4 cut(s)	92, 104, 316, 2430
GsuI	CTGGAG	1 cut(s)	1022
HaeII	RGCGCY	1 cut(s)	318
HapII	CCGG	7 cut(s)	240, 301, 348, 354, 818, 1726, 2200
HgaI	GACGC	4 cut(s)	94, 1285, 1709, 1999
HhaI	GCGC	4 cut(s)	93, 105, 317, 2431
Hin1I	GRCGYC	2 cut(s)	123, 315
Hin6I	GCGC	4 cut(s)	91, 103, 315, 2429
HinP1I	GCGC	4 cut(s)	91, 103, 315, 2429
HincII	GTYRAC	4 cut(s)	208, 268, 1708, 2521
HindII	GTYRAC	4 cut(s)	208, 268, 1708, 2521
HpaII	CCGG	7 cut(s)	240, 301, 348, 354, 818, 1726, 2200
HphI	GGTGA	6 cut(s)	205, 1316, 1715, 1869, 2023, 2068
Hpy166II	GTNNAC	6 cut(s)	208, 268, 952, 1708, 1975, 2521
Hpy188I	TCNGA	7 cut(s)	154, 375, 541, 1543, 1989, 2331, 2487
Hpy188III	TCNNGA	9 cut(s)	14, 604, 991, 1001, 1082, 1105, 2171, 2222, 2393
Hpy8I	GTNNAC	6 cut(s)	208, 268, 952, 1708, 1975, 2521
Hpy99I	CGWCG	8 cut(s)	125, 158, 161, 164, 176, 272, 413, 416
HpyCH4III	ACNGT	3 cut(s)	1234, 1735, 1881
HpyCH4IV	ACGT	2 cut(s)	123, 1068
HpyF3I	CTNAG	8 cut(s)	525, 879, 978, 1087, 1092, 1124, 1415, 2457
HpySE526I	ACGT	2 cut(s)	123, 1068
Hsp92I	GRCGYC	2 cut(s)	123, 315
HspAI	GCGC	4 cut(s)	91, 103, 315, 2429
KasI	GGCGCC	1 cut(s)	314
Ksp22I	TGATCA	1 cut(s)	1996
Kzo9I	GATC	6 cut(s)	63, 382, 1240, 1856, 1984, 1996
LmnI	GCTCC	5 cut(s)	461, 566, 700, 1370, 2176
LweI	GCATC	5 cut(s)	624, 811, 1765, 1888, 2460
MabI	ACCWGGT	1 cut(s)	2266
MaeI	CTAG	2 cut(s)	513, 774
MaeII	ACGT	2 cut(s)	123, 1068
MaeIII	GTNAC	2 cut(s)	1304, 1343
MalI	GATC	6 cut(s)	65, 384, 1242, 1858, 1986, 1998
MbiI	CCGCTC	2 cut(s)	464, 1135
MboI	GATC	6 cut(s)	63, 382, 1240, 1856, 1984, 1996
MfeI	CAATTG	1 cut(s)	1532
MflI	RGATCY	2 cut(s)	382, 1856
MhlI	GDGCHC	2 cut(s)	938, 954
MlsI	TGGCCA	1 cut(s)	1190
MluNI	TGGCCA	1 cut(s)	1190
Mly113I	GGCGCC	1 cut(s)	315
MlyI	GAGTC	4 cut(s)	996, 1277, 1634, 2516
MmeI	TCCRAC	6 cut(s)	177, 1155, 1416, 1767, 2135, 2287
Mox20I	TGGCCA	1 cut(s)	1190
Mph1103I	ATGCAT	1 cut(s)	2475
MroXI	GAANNNNTTC	2 cut(s)	2069, 2166
MscI	TGGCCA	1 cut(s)	1190
MseI	TTAA	5 cut(s)	366, 639, 2138, 2211, 2613
MslI	CAYNNNNRTG	4 cut(s)	626, 1745, 1848, 2187
Msp20I	TGGCCA	1 cut(s)	1190
MspA1I	CMGCKG	1 cut(s)	866
MspCI	CTTAAG	1 cut(s)	638
MspI	CCGG	7 cut(s)	240, 301, 348, 354, 818, 1726, 2200
MunI	CAATTG	1 cut(s)	1532
Mva1269I	GAATGC	2 cut(s)	687, 1018
MvnI	CGCG	1 cut(s)	105
NarI	GGCGCC	1 cut(s)	315
NciI	CCSGG	1 cut(s)	1727
NdeII	GATC	6 cut(s)	63, 382, 1240, 1856, 1984, 1996
NmeAIII	GCCGAG	3 cut(s)	291, 309, 828
NmuCI	GTSAC	1 cut(s)	1304
NsbI	TGCGCA	1 cut(s)	2430
NsiI	ATGCAT	1 cut(s)	2475
NspI	RCATGY	1 cut(s)	2599
OliI	CACNNNNGTG	1 cut(s)	1848
PagI	TCATGA	1 cut(s)	2392
PceI	AGGCCT	1 cut(s)	2455
PcsI	WCGNNNNNNNCGW	1 cut(s)	168
PctI	GAATGC	2 cut(s)	687, 1018
PdmI	GAANNNNTTC	2 cut(s)	2069, 2166
PfeI	GAWTC	6 cut(s)	149, 243, 1078, 1408, 1812, 2233
PflFI	GACNNNGTC	1 cut(s)	122
PflMI	CCANNNNNTGG	1 cut(s)	1853
PfoI	TCCNGGA	1 cut(s)	848
PinAI	ACCGGT	1 cut(s)	347
PleI	GAGTC	4 cut(s)	995, 1277, 1633, 2516
PluTI	GGCGCC	1 cut(s)	318
PpsI	GAGTC	4 cut(s)	995, 1277, 1633, 2516
PpuMI	RGGWCCY	3 cut(s)	974, 1120, 1597
PshAI	GACNNNNGTC	1 cut(s)	761
Psp5II	RGGWCCY	3 cut(s)	974, 1120, 1597
PspEI	GGTNACC	1 cut(s)	1304
PspPPI	RGGWCCY	3 cut(s)	974, 1120, 1597
PsrI	GAACNNNNNNTAC	2 cut(s)	635, 667
PstI	CTGCAG	2 cut(s)	1314, 1984
PsuI	RGATCY	2 cut(s)	382, 1856
PsyI	GACNNNGTC	1 cut(s)	122
RsaI	GTAC	4 cut(s)	1493, 1554, 1938, 2368
RsaNI	GTAC	4 cut(s)	1492, 1553, 1937, 2367
RseI	CAYNNNNRTG	4 cut(s)	626, 1745, 1848, 2187
SalI	GTCGAC	2 cut(s)	206, 266
SaqAI	TTAA	5 cut(s)	366, 639, 2138, 2211, 2613
Sau3AI	GATC	6 cut(s)	63, 382, 1240, 1856, 1984, 1996
ScaI	AGTACT	1 cut(s)	1493
SchI	GAGTC	4 cut(s)	996, 1277, 1634, 2516
SduI	GDGCHC	2 cut(s)	938, 954
SexAI	ACCWGGT	1 cut(s)	2266
SfaNI	GCATC	5 cut(s)	624, 811, 1765, 1888, 2460
SfcI	CTRYAG	4 cut(s)	1058, 1310, 1980, 2087
SfoI	GGCGCC	1 cut(s)	316
SinI	GGWCC	7 cut(s)	886, 974, 1120, 1230, 1597, 2260, 2565
SmiMI	CAYNNNNRTG	4 cut(s)	626, 1745, 1848, 2187
SmlI	CTYRAG	3 cut(s)	503, 638, 2303
SmoI	CTYRAG	3 cut(s)	503, 638, 2303
SpeI	ACTAGT	1 cut(s)	512
SseBI	AGGCCT	1 cut(s)	2455
SspDI	GGCGCC	1 cut(s)	314
SspI	AATATT	1 cut(s)	2284
SspMI	CTAG	2 cut(s)	513, 774
StuI	AGGCCT	1 cut(s)	2455
StyI	CCWWGG	3 cut(s)	615, 903, 1593
TaaI	ACNGT	3 cut(s)	1234, 1735, 1881
TaiI	ACGT	2 cut(s)	126, 1071
TaqI	TCGA	6 cut(s)	207, 267, 532, 603, 985, 1243
TaqII	GACCGA	1 cut(s)	213
TatI	WGTACW	1 cut(s)	1491
TauI	GCSGC	4 cut(s)	344, 579, 772, 866
TfiI	GAWTC	6 cut(s)	149, 243, 1078, 1408, 1812, 2233
Tru1I	TTAA	5 cut(s)	366, 639, 2138, 2211, 2613
Tru9I	TTAA	5 cut(s)	366, 639, 2138, 2211, 2613
TscAI	CASTG	4 cut(s)	65, 148, 1738, 2108
TseFI	GTSAC	1 cut(s)	1304
Tsp45I	GTSAC	1 cut(s)	1304
TspDTI	ATGAA	9 cut(s)	220, 644, 1457, 1649, 2030, 2171, 2205, 2246, 2414
TspGWI	ACGGA	4 cut(s)	375, 773, 1196, 1749
TspRI	CASTG	4 cut(s)	65, 148, 1738, 2108
Tth111I	GACNNNGTC	1 cut(s)	122
Van91I	CCANNNNNTGG	1 cut(s)	1853
Vha464I	CTTAAG	1 cut(s)	638
VneI	GTGCAC	1 cut(s)	950
VpaK11BI	GGWCC	7 cut(s)	886, 974, 1120, 1230, 1597, 2260, 2565
XagI	CCTNNNNNAGG	1 cut(s)	1292
XapI	RAATTY	7 cut(s)	369, 376, 1006, 1964, 2066, 2401, 2605
XceI	RCATGY	1 cut(s)	2599
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1672
XmiI	GTMKAC	2 cut(s)	207, 267
XmnI	GAANNNNTTC	2 cut(s)	2069, 2166
XspI	CTAG	2 cut(s)	513, 774
ZraI	GACGTC	1 cut(s)	124
ZrmI	AGTACT	1 cut(s)	1493
Zsp2I	ATGCAT	1 cut(s)	2475

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.