Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002460

Physical Location & Seq

Genomic Coordinates Reverse (-)

54697493 .. 54700346

Gene Structure 2854 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1445100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2628 bp

ATGGATGATTATCAGGAGATGGAGAGGTTTGGGATGGAGGAGGATTACGAGGACGCCCAGTGGATCGGCGGCGAGTTCTATTACCGCAAGCGCAAGGACAAGCCCGTCCAAACCAAAGACGACGTCCTCTACGGCATATTTACTGCCGATTCCGATGACGACGATGACAACGACGGCTCCAGAAAACGCAGGAAAGACCGAAGGGTCGACCTCACCAAGCCCGTCAGTTTTGTCTCCACAGGAATCGTCATGCCCAACCAGGAGGTCGACGACAATTCAAAGCAGCAAAACGATGATAAGCGGCCTGGCCTCGGCGCCACCAATGGCCTTGGATTTGGTGCCGCTACGGGTTCCGGTTTAGGTTTTAAAAATCCGAATTCGGATTCTTTGGGTGGCGAGGAGGAGGAGGAGGAGGTGGAGGATGAGCAGAGTTTTTTACCGACGGCTTTTGGGAAGAAGATAAAGGAGGGAGCGGAGAGGAGACACAAGGAGAGGGAAAAGATGAAATTGCTCAAGCTTACTAGTCGTCAGTCTCAGAGTAGAGGAGTTTCTGAAGAACGTCAATTTGCAGGACTAGGAGCAAAGGGCGGTGGGGATGGGGGTCTGGGGGCCTTCGAGAAACACACCAAGGGCATTGGAATGAAGATGCTTAAGAACATGGGCTACAAAGGAGGCGGACTTGGGAAGAACGAGCAAGGCATTCTCGCTCCTATTGAAGCCAAGCTGAGGCCAAAGAATATGGGCATGGGTTTCAATGATTACAAGGAGACGGAGGTCAAGCGGCCTAGCTTGCAAGAATTGGAAGCTGAAAAGCCGAGCAAGCCTTTGCCCGCTGCCACCTCCACCAAAAAGAAGCCTTCCTGGAAGAAGAACAAGACTAACAAGGACCATTATGTCTCTGCCAAGGAGTTGTTAGCCCAGAAGCAGGAAGAAGGCACAGAGGTCCTTGTGCACAAGGTGGTTGATATGCGCGGACCTCAGGTTCGAGTCTTGACCAATCTGGAGAATTTGAATGCCGAAGAGAAAGCAAGGGAAGAGGATCTTCCCATGCCTGAGCTACAGCACAACGTCAGGTTGATTCTGGACTTAGCTGAGCTCGATATCCAGAAGATTGACAGGGACCTGAGGAATGAGCGGGACACGGCCATCAGCTTGAACCAGGAGAAAGAGAGGTTGGAGACGGAGGTGGCCAGGCAGAAGCAACACCTGGATAACTTGGAGGATATTAGGACCGTCATAGATCGACTGGGAGAAGAGAATAGCATGGGAACATTGACGCTGGACTCCCTGACAAAGGGCTTTCGTGACCTGCAGAAGAGATACGCCGATGACTATAAGTTATGTAACTTGTCTTGCATTGCCTGCTCCTTCGCTCTGCCTCTGTTTATTAGGATGTTTCAAGGCTGGGATCCTCTTAGGAACCCCTCGCATGGGTTGGATGTAATATCTTCTTGGAAGGCTTTGCTTCATGGAGAGGGGGAGAGCGAGCAATACCTGGATATTTGGGATAGCTCAATGTCCCCTTACACCAAATTGCTTTCCGAGGTGGTGGTACCCGCCGTGAGGATTGCTGGCGTCAATACATGGCAGCCCAAGGACCCTGAACCCATGCTTCGCTTTCTGGAGTCTTGGGAGAACTTGCTGCCTCCTTCTGTCCTTCATTCCATACTGGATATGGTAATCTTTCCTAAATTGAAGGACGCAGTTGACTTGTGGGAACCTCACCGGGACACTGTTCCCATCCATGTGTGGGTGCATCCGTGGCTACCACTCTTGGGACACAAGTTGGAAGACTTGTATCACACAATACGATTCAAGTTGAGTAATGTTCTGGGTGCTTGGCACCCAAGCGATGGATCTGCCTATACCATACTCTCACCGTGGAAGAAGGTGTTTGATGCCGCGAGTTGGGAACAACTTATGTATAGATTTATAGTACCCAAGTTGCAGCTTGTCTTACAAGAATTCCAAGTGAACCCTGCAGATCAGACACTTGATCAATTTAATTGGGTGATGAGTTGGGCTTCTGCTATTCCAATTCATCTAATGGTAGATATATTGGAGAAATTTTTCTTCACCAAGTGGCTACAGGTTTTGTATCACTGGTTATGTTCGAACCCGAACTTTGAAGAGGTTTTAAATTGGTACAAAGGTTGGAAGGAACTCATTCCTGAGGAGCTTCATGCAAATGAAAGTATCCGGTATCAGCTTAATTGTGGTCTTGACATGATGAATCGGGCTGTTGAGGGTATGGAGGTGGTCCAACCTGGTTTGAAGGAGAATATTAGCTACCTTAGGGTGCTTGAGCAAAGGCAATTTGAGGCACAGCAGAAAGCAGCAGCAGCAGCACATGCAAACTTGGGTGGCACTGCTCAAATGGATGGCATTGGTCACGAGATGAGTTTGAAAGATGTTATTGAAGCCCATGCGCAGCAAAATGGGCTGCTATTTAGGCCTAAGCCTGGGAGGATGCATAATGGTCACCAGATGTATGGCTTTGGTAATGTAAGCATAATAGTTGATTCCCTAAATCAAAAGGTATATGCCCAAACAGAGGAAACTTGGTCCCTTGTATCCCTTGAAAGGTTGCTGGACATGCACAATAATTTACTTACAAGGAGACGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000375 GO:0000377 GO:0000390 GO:0000398 GO:0000781 GO:0000784 GO:0003674 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005694 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0022411 GO:0030154 GO:0030198 GO:0031012 GO:0031333 GO:0031974 GO:0031981 GO:0032091 GO:0032502 GO:0032984 GO:0032988 GO:0032991 GO:0034641 GO:0042752 GO:0043021 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043393 GO:0043933 GO:0044087 GO:0044092 GO:0044237 GO:0044238 GO:0044421 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0046483 GO:0048519 GO:0048523 GO:0048869 GO:0050789 GO:0050794 GO:0051098 GO:0051100 GO:0051128 GO:0051129 GO:0065007 GO:0065009 GO:0070013 GO:0070990 GO:0071008 GO:0071013 GO:0071014 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0098687 GO:1901360 GO:1902494 GO:1990446 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-patch GCFC TIP_N

Protein Analysis

876

Amino Acids

99.96

Weight (kDa)

5.66

Isoelectric Point (pI)

46.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIP_N	PF12457	2 - 100	3.4e-21	Tuftelin interacting protein N terminal
G-patch	PF01585	209 - 250	5e-14	G-patch domain
GCFC	PF07842	427 - 699	9.5e-93	GC-rich sequence DNA-binding factor-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002460)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17070
fragaria_vesca	FvH4_2g25582 FvH4_5g27320
malus_domestica	MD08G1250100.v1.1 MD15G1445100.v1.1
prunus_persica	Prupe.1G587200_v2.0.a1 Prupe.5G048700_v2.0.a1
pyrus_communis	pycom08g21680 pycom15g39220
rosa_chinensis	RchiOBHm_Chr6g0293801 RchiOBHm_Chr6g0293811 RchiOBHm_Chr7g0218481
rosa_laevigata	RLG00000002370 RLG00000011921 RLG00000012034
rosa_multiflora	Rmu_co8485319.1_g000001 Rmu_sc0001301.1_g000062 Rmu_sc0001301.1_g000063 Rmu_sc0001354.1_g000013 Rmu_sc0023058.1_g000001
rosa_roxburghii	Rroxscaffold_3G00240910 Rroxscaffold_7G00173890 Rroxscaffold_7G00175120
rosa_rugosa	Rorug06G0238200 Rorug07G0177400
rosa_samantha	Rh6AG339400 Rh6AG351600 Rh6BG346700 Rh6BG358400 Rh6CG353500 Rh6CG365600 Rh6DG339500 Rh6DG352200 Rh6DG352300 Rh7AG318900 Rh7BG310800 Rh7CG335800 Rh7DG318000
rosa_wichuraiana	Rw6G029540 Rw6G030580 Rw7G027140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	104, 203, 893
AatII	GACGTC	1 cut(s)	126
Acc16I	TGCGCA	1 cut(s)	2430
Acc36I	ACCTGC	1 cut(s)	1317
Acc65I	GGTACC	1 cut(s)	1552
AccB1I	GGYRCC	4 cut(s)	314, 338, 1552, 1842
AccB7I	CCANNNNNTGG	1 cut(s)	1853
AccBSI	CCGCTC	2 cut(s)	473, 1135
AccI	GTMKAC	2 cut(s)	207, 267
AccII	CGCG	2 cut(s)	972, 1904
AclWI	GGATC	5 cut(s)	71, 1047, 1403, 1416, 1864
AcoI	YGGCCR	2 cut(s)	1143, 1188
AcsI	RAATTY	4 cut(s)	376, 1006, 1964, 2066
AcuI	CTGAAG	1 cut(s)	573
AcyI	GRCGYC	4 cut(s)	54, 123, 315, 1575
AdeI	CACNNNGTG	2 cut(s)	958, 2082
AfaI	GTAC	3 cut(s)	1554, 1938, 2147
AflII	CTTAAG	1 cut(s)	650
AhlI	ACTAGT	1 cut(s)	521
AjnI	CCWGG	9 cut(s)	258, 304, 860, 1158, 1190, 1206, 1494, 2266, 2461
AjuI	GAANNNNNNNTTGG	2 cut(s)	1157, 1189
AloI	GAACNNNNNNTCC	2 cut(s)	966, 998
Alw21I	GWGCWC	2 cut(s)	954, 1098
Alw26I	GTCTC	7 cut(s)	238, 475, 537, 761, 901, 1172, 2614
Alw44I	GTGCAC	1 cut(s)	950
AlwI	GGATC	5 cut(s)	71, 1047, 1403, 1416, 1864
AoxI	GGCC	9 cut(s)	302, 307, 325, 609, 728, 782, 1143, 1188, 2453
ApaLI	GTGCAC	1 cut(s)	950
ApoI	RAATTY	4 cut(s)	376, 1006, 1964, 2066
ArsI	GACNNNNNNTTYG	4 cut(s)	108, 140, 966, 998
Asp700I	GAANNNNTTC	2 cut(s)	2069, 2166
Asp718I	GGTACC	1 cut(s)	1552
AspLEI	GCGC	4 cut(s)	93, 317, 972, 2431
AspS9I	GGNCC	9 cut(s)	609, 886, 943, 974, 1120, 1230, 1597, 2260, 2565
AsuC2I	CCSGG	1 cut(s)	1727
AsuHPI	GGTGA	6 cut(s)	205, 1715, 1869, 2023, 2068, 2474
AsuII	TTCGAA	1 cut(s)	2114
AvaII	GGWCC	8 cut(s)	886, 943, 974, 1120, 1230, 1597, 2260, 2565
AxyI	CCTNAGG	4 cut(s)	978, 1124, 2172, 2294
BaeGI	GKGCMC	1 cut(s)	954
BaeI	ACNNNNGTAYC	2 cut(s)	2137, 2170
BalI	TGGCCA	1 cut(s)	1190
BamHI	GGATCC	1 cut(s)	1408
BanI	GGYRCC	4 cut(s)	314, 338, 1552, 1842
BanII	GRGCYC	1 cut(s)	1098
BauI	CACGAG	1 cut(s)	2393
BbsI	GAAGAC	1 cut(s)	1797
Bbv12I	GWGCWC	2 cut(s)	954, 1098
BbvCI	CCTCAGC	1 cut(s)	725
BccI	CCATC	7 cut(s)	13, 28, 590, 1154, 1748, 1847, 2375
BceAI	ACGGC	5 cut(s)	148, 190, 459, 1158, 1544
BcgI	CGANNNNNNTGC	2 cut(s)	1841, 1875
BciT130I	CCWGG	9 cut(s)	260, 306, 862, 1160, 1192, 1208, 1496, 2268, 2463
BciVI	GTATCC	2 cut(s)	2207, 2584
BclI	TGATCA	1 cut(s)	1996
BcnI	CCSGG	1 cut(s)	1727
BcoDI	GTCTC	7 cut(s)	238, 475, 537, 761, 901, 1172, 2614
BcuI	ACTAGT	1 cut(s)	521
BfaI	CTAG	3 cut(s)	522, 575, 786
BfmI	CTRYAG	4 cut(s)	1058, 1310, 1980, 2087
BfoI	RGCGCY	1 cut(s)	318
BfrI	CTTAAG	1 cut(s)	650
BfuAI	ACCTGC	1 cut(s)	1317
BfuI	GTATCC	2 cut(s)	2207, 2584
BlpI	GCTNAGC	1 cut(s)	1092
Bme18I	GGWCC	8 cut(s)	886, 943, 974, 1120, 1230, 1597, 2260, 2565
BmgT120I	GGNCC	9 cut(s)	609, 886, 943, 974, 1120, 1230, 1597, 2260, 2565
BmrI	ACTGGG	2 cut(s)	52, 1256
BmsI	GCATC	4 cut(s)	636, 1765, 1888, 2460
BmuI	ACTGGG	2 cut(s)	52, 1256
BoxI	GACNNNNGTC	1 cut(s)	773
BpiI	GAAGAC	1 cut(s)	1797
BpmI	CTGGAG	3 cut(s)	163, 1022, 1643
Bpu10I	CCTNAGC	3 cut(s)	725, 1053, 2457
Bpu1102I	GCTNAGC	1 cut(s)	1092
Bpu14I	TTCGAA	1 cut(s)	2114
BpuEI	CTTGAG	2 cut(s)	497, 2324
BpuMI	CCSGG	1 cut(s)	1727
BsaBI	GATNNNNATC	1 cut(s)	9
BsaHI	GRCGYC	4 cut(s)	54, 123, 315, 1575
BsaJI	CCNNGG	9 cut(s)	310, 328, 627, 903, 1542, 1593, 1760, 1880, 2462
BsaWI	WCCGGW	2 cut(s)	353, 2199
BsaXI	ACNNNNNCTCC	2 cut(s)	161, 191
Bse1I	ACTGG	4 cut(s)	58, 1251, 1674, 2108
Bse21I	CCTNAGG	4 cut(s)	978, 1124, 2172, 2294
Bse3DI	GCAATG	1 cut(s)	1356
Bse8I	GATNNNNATC	1 cut(s)	9
BseBI	CCWGG	9 cut(s)	260, 306, 862, 1160, 1192, 1208, 1496, 2268, 2463
BseDI	CCNNGG	9 cut(s)	310, 328, 627, 903, 1542, 1593, 1760, 1880, 2462
BseJI	GATNNNNATC	1 cut(s)	9
BseMI	GCAATG	1 cut(s)	1356
BseMII	CTCAG	7 cut(s)	548, 716, 992, 1044, 1083, 1115, 2163
BseNI	ACTGG	4 cut(s)	58, 1251, 1674, 2108
BseRI	GAGGAG	9 cut(s)	53, 413, 416, 419, 422, 425, 493, 558, 2189
BseSI	GKGCMC	1 cut(s)	954
BseYI	CCCAGC	1 cut(s)	1404
Bsh1236I	CGCG	2 cut(s)	972, 1904
BshFI	GGCC	9 cut(s)	304, 309, 327, 611, 730, 784, 1145, 1190, 2455
BshNI	GGYRCC	4 cut(s)	314, 338, 1552, 1842
BsiHKAI	GWGCWC	2 cut(s)	954, 1098
BsiSI	CCGG	3 cut(s)	354, 1726, 2200
BslFI	GGGAC	6 cut(s)	1133, 1151, 1504, 1742, 1791, 2551
BsmAI	GTCTC	7 cut(s)	238, 475, 537, 761, 901, 1172, 2614
BsmBI	CGTCTC	3 cut(s)	761, 1172, 2614
BsmFI	GGGAC	6 cut(s)	1133, 1151, 1504, 1742, 1791, 2551
BsmI	GAATGC	2 cut(s)	699, 1018
BsnI	GGCC	9 cut(s)	304, 309, 327, 611, 730, 784, 1145, 1190, 2455
Bsp119I	TTCGAA	1 cut(s)	2114
Bsp1286I	GDGCHC	2 cut(s)	954, 1098
Bsp143I	GATC	7 cut(s)	63, 1039, 1240, 1408, 1856, 1984, 1996
Bsp1720I	GCTNAGC	1 cut(s)	1092
BspANI	GGCC	9 cut(s)	304, 309, 327, 611, 730, 784, 1145, 1190, 2455
BspCNI	CTCAG	7 cut(s)	547, 717, 991, 1045, 1084, 1116, 2164
BspFNI	CGCG	2 cut(s)	972, 1904
BspMAI	CTGCAG	2 cut(s)	1314, 1984
BspMI	ACCTGC	1 cut(s)	1317
BspPI	GGATC	5 cut(s)	71, 1047, 1403, 1416, 1864
BspT104I	TTCGAA	1 cut(s)	2114
BspT107I	GGYRCC	4 cut(s)	314, 338, 1552, 1842
BspTI	CTTAAG	1 cut(s)	650
BsrBI	CCGCTC	2 cut(s)	473, 1135
BsrDI	GCAATG	1 cut(s)	1356
BsrI	ACTGG	4 cut(s)	58, 1251, 1674, 2108
BssECI	CCNNGG	9 cut(s)	310, 328, 627, 903, 1542, 1593, 1760, 1880, 2462
BssMI	GATC	7 cut(s)	63, 1039, 1240, 1408, 1856, 1984, 1996
BssNI	GRCGYC	4 cut(s)	54, 123, 315, 1575
BssSI	CACGAG	1 cut(s)	2393
BssT1I	CCWWGG	4 cut(s)	328, 627, 903, 1593
Bst2BI	CACGAG	1 cut(s)	2393
Bst2UI	CCWGG	9 cut(s)	260, 306, 862, 1160, 1192, 1208, 1496, 2268, 2463
Bst4CI	ACNGT	3 cut(s)	1234, 1735, 1881
Bst6I	CTCTTC	5 cut(s)	1014, 1029, 1248, 1310, 2124
BstACI	GRCGYC	4 cut(s)	54, 123, 315, 1575
BstAFI	CTTAAG	1 cut(s)	650
BstAPI	GCANNNNNTGC	2 cut(s)	1362, 2351
BstBI	TTCGAA	1 cut(s)	2114
BstC8I	GCNNGC	7 cut(s)	89, 791, 821, 831, 1363, 1487, 1573
BstDSI	CCRYGG	2 cut(s)	1760, 1880
BstEII	GGTNACC	1 cut(s)	2480
BstENI	CCTNNNNNAGG	1 cut(s)	1292
BstFNI	CGCG	2 cut(s)	972, 1904
BstH2I	RGCGCY	1 cut(s)	318
BstHHI	GCGC	4 cut(s)	93, 317, 972, 2431
BstKTI	GATC	7 cut(s)	66, 1042, 1243, 1411, 1859, 1987, 1999
BstMAI	GTCTC	7 cut(s)	238, 475, 537, 761, 901, 1172, 2614
BstMBI	GATC	7 cut(s)	63, 1039, 1240, 1408, 1856, 1984, 1996
BstNI	CCWGG	9 cut(s)	260, 306, 862, 1160, 1192, 1208, 1496, 2268, 2463
BstNSI	RCATGY	2 cut(s)	2354, 2599
BstPAI	GACNNNNGTC	1 cut(s)	773
BstPI	GGTNACC	1 cut(s)	2480
BstSFI	CTRYAG	4 cut(s)	1058, 1310, 1980, 2087
BstSLI	GKGCMC	1 cut(s)	954
BstUI	CGCG	2 cut(s)	972, 1904
BstV2I	GAAGAC	1 cut(s)	1797
BstX2I	RGATCY	3 cut(s)	1039, 1408, 1856
BstXI	CCANNNNNNTGG	1 cut(s)	2492
BstYI	RGATCY	3 cut(s)	1039, 1408, 1856
Bsu36I	CCTNAGG	4 cut(s)	978, 1124, 2172, 2294
BsuI	GTATCC	2 cut(s)	2207, 2584
BsuRI	GGCC	9 cut(s)	304, 309, 327, 611, 730, 784, 1145, 1190, 2455
BtgI	CCRYGG	2 cut(s)	1760, 1880
BtgZI	GCGATG	1 cut(s)	1866
BtsI	GCAGTG	1 cut(s)	2367
BtsIMutI	CAGTG	4 cut(s)	65, 1731, 2101, 2367
BveI	ACCTGC	1 cut(s)	1317
Cac8I	GCNNGC	7 cut(s)	89, 791, 821, 831, 1363, 1487, 1573
CfoI	GCGC	4 cut(s)	93, 317, 972, 2431
Cfr13I	GGNCC	9 cut(s)	609, 886, 943, 974, 1120, 1230, 1597, 2260, 2565
CseI	GACGC	4 cut(s)	62, 1285, 1564, 1709
CsiI	ACCWGGT	1 cut(s)	2266
Csp6I	GTAC	3 cut(s)	1553, 1937, 2146
CviQI	GTAC	3 cut(s)	1553, 1937, 2146
DinI	GGCGCC	1 cut(s)	316
DpnI	GATC	7 cut(s)	65, 1041, 1242, 1410, 1858, 1986, 1998
DpnII	GATC	7 cut(s)	63, 1039, 1240, 1408, 1856, 1984, 1996
DraI	TTTAAA	2 cut(s)	367, 2139
DraIII	CACNNNGTG	2 cut(s)	958, 2082
DrdI	GACNNNNNNGTC	3 cut(s)	104, 203, 893
DseDI	GACNNNNNNGTC	3 cut(s)	104, 203, 893
EaeI	YGGCCR	2 cut(s)	1143, 1188
Eam1104I	CTCTTC	5 cut(s)	1014, 1029, 1248, 1310, 2124
EarI	CTCTTC	5 cut(s)	1014, 1029, 1248, 1310, 2124
EciI	GGCGGA	1 cut(s)	690
Ecl136II	GAGCTC	1 cut(s)	1096
Eco130I	CCWWGG	4 cut(s)	328, 627, 903, 1593
Eco147I	AGGCCT	1 cut(s)	2455
Eco24I	GRGCYC	1 cut(s)	1098
Eco32I	GATATC	1 cut(s)	1102
Eco47I	GGWCC	8 cut(s)	886, 943, 974, 1120, 1230, 1597, 2260, 2565
Eco53kI	GAGCTC	1 cut(s)	1096
Eco57I	CTGAAG	1 cut(s)	573
Eco81I	CCTNAGG	4 cut(s)	978, 1124, 2172, 2294
Eco91I	GGTNACC	1 cut(s)	2480
EcoICRI	GAGCTC	1 cut(s)	1096
EcoNI	CCTNNNNNAGG	1 cut(s)	1292
EcoO109I	RGGNCCY	4 cut(s)	609, 943, 1120, 1597
EcoO65I	GGTNACC	1 cut(s)	2480
EcoRI	GAATTC	2 cut(s)	376, 1964
EcoRII	CCWGG	9 cut(s)	258, 304, 860, 1158, 1190, 1206, 1494, 2266, 2461
EcoRV	GATATC	1 cut(s)	1102
EcoT14I	CCWWGG	4 cut(s)	328, 627, 903, 1593
EcoT22I	ATGCAT	1 cut(s)	2475
EcoT38I	GRGCYC	1 cut(s)	1098
EgeI	GGCGCC	1 cut(s)	316
EheI	GGCGCC	1 cut(s)	316
ErhI	CCWWGG	4 cut(s)	328, 627, 903, 1593
Esp3I	CGTCTC	3 cut(s)	761, 1172, 2614
FalI	AAGNNNNNCTT	2 cut(s)	1026, 1058
FaqI	GGGAC	6 cut(s)	1133, 1151, 1504, 1742, 1791, 2551
FauI	CCCGC	3 cut(s)	838, 1128, 1564
FbaI	TGATCA	1 cut(s)	1996
FblI	GTMKAC	2 cut(s)	207, 267
FriOI	GRGCYC	1 cut(s)	1098
FspBI	CTAG	3 cut(s)	522, 575, 786
FspI	TGCGCA	1 cut(s)	2430
GlaI	GCGC	4 cut(s)	92, 316, 971, 2430
GsaI	CCCAGC	1 cut(s)	1408
GsuI	CTGGAG	3 cut(s)	163, 1022, 1643
HaeII	RGCGCY	1 cut(s)	318
HaeIII	GGCC	9 cut(s)	304, 309, 327, 611, 730, 784, 1145, 1190, 2455
HapII	CCGG	3 cut(s)	354, 1726, 2200
HgaI	GACGC	4 cut(s)	62, 1285, 1564, 1709
HhaI	GCGC	4 cut(s)	93, 317, 972, 2431
Hin1I	GRCGYC	4 cut(s)	54, 123, 315, 1575
Hin6I	GCGC	4 cut(s)	91, 315, 970, 2429
HinP1I	GCGC	4 cut(s)	91, 315, 970, 2429
HincII	GTYRAC	3 cut(s)	208, 268, 1708
HindII	GTYRAC	3 cut(s)	208, 268, 1708
HindIII	AAGCTT	1 cut(s)	515
HpaII	CCGG	3 cut(s)	354, 1726, 2200
HphI	GGTGA	6 cut(s)	205, 1715, 1869, 2023, 2068, 2474
Hpy166II	GTNNAC	5 cut(s)	208, 268, 952, 1708, 1975
Hpy188I	TCNGA	7 cut(s)	154, 375, 382, 537, 553, 1543, 1989
Hpy8I	GTNNAC	5 cut(s)	208, 268, 952, 1708, 1975
Hpy99I	CGWCG	5 cut(s)	125, 164, 176, 272, 445
HpyCH4III	ACNGT	3 cut(s)	1234, 1735, 1881
HpyCH4IV	ACGT	3 cut(s)	123, 559, 1068
HpySE526I	ACGT	3 cut(s)	123, 559, 1068
Hsp92I	GRCGYC	4 cut(s)	54, 123, 315, 1575
HspAI	GCGC	4 cut(s)	91, 315, 970, 2429
KasI	GGCGCC	1 cut(s)	314
KpnI	GGTACC	1 cut(s)	1556
Ksp22I	TGATCA	1 cut(s)	1996
Kzo9I	GATC	7 cut(s)	63, 1039, 1240, 1408, 1856, 1984, 1996
LmnI	GCTCC	6 cut(s)	182, 470, 578, 712, 1370, 2176
LweI	GCATC	4 cut(s)	636, 1765, 1888, 2460
MabI	ACCWGGT	1 cut(s)	2266
MaeI	CTAG	3 cut(s)	522, 575, 786
MaeII	ACGT	3 cut(s)	123, 559, 1068
MaeIII	GTNAC	4 cut(s)	1304, 1343, 2390, 2480
MalI	GATC	7 cut(s)	65, 1041, 1242, 1410, 1858, 1986, 1998
MbiI	CCGCTC	2 cut(s)	473, 1135
MboI	GATC	7 cut(s)	63, 1039, 1240, 1408, 1856, 1984, 1996
MflI	RGATCY	3 cut(s)	1039, 1408, 1856
MhlI	GDGCHC	2 cut(s)	954, 1098
MlsI	TGGCCA	1 cut(s)	1190
MluNI	TGGCCA	1 cut(s)	1190
Mly113I	GGCGCC	1 cut(s)	315
MlyI	GAGTC	3 cut(s)	996, 1277, 1634
MmeI	TCCRAC	5 cut(s)	1155, 1416, 1767, 2135, 2287
Mox20I	TGGCCA	1 cut(s)	1190
Mph1103I	ATGCAT	1 cut(s)	2475
MroXI	GAANNNNTTC	2 cut(s)	2069, 2166
MscI	TGGCCA	1 cut(s)	1190
MseI	TTAA	5 cut(s)	366, 651, 2004, 2138, 2211
MslI	CAYNNNNRTG	3 cut(s)	638, 1745, 2187
Msp20I	TGGCCA	1 cut(s)	1190
MspA1I	CMGCKG	1 cut(s)	833
MspCI	CTTAAG	1 cut(s)	650
MspI	CCGG	3 cut(s)	354, 1726, 2200
Mva1269I	GAATGC	2 cut(s)	699, 1018
MvaI	CCWGG	9 cut(s)	260, 306, 862, 1160, 1192, 1208, 1496, 2268, 2463
MvnI	CGCG	2 cut(s)	972, 1904
NarI	GGCGCC	1 cut(s)	315
NciI	CCSGG	1 cut(s)	1727
NdeII	GATC	7 cut(s)	63, 1039, 1240, 1408, 1856, 1984, 1996
NmeAIII	GCCGAG	2 cut(s)	291, 840
NmuCI	GTSAC	3 cut(s)	1304, 2390, 2480
NsbI	TGCGCA	1 cut(s)	2430
NsiI	ATGCAT	1 cut(s)	2475
NspI	RCATGY	2 cut(s)	2354, 2599
NspV	TTCGAA	1 cut(s)	2114
PceI	AGGCCT	1 cut(s)	2455
PcsI	WCGNNNNNNNCGW	1 cut(s)	168
PctI	GAATGC	2 cut(s)	699, 1018
PdmI	GAANNNNTTC	2 cut(s)	2069, 2166
PfeI	GAWTC	7 cut(s)	149, 243, 383, 1078, 1812, 2233, 2522
PflFI	GACNNNGTC	1 cut(s)	122
PflMI	CCANNNNNTGG	1 cut(s)	1853
PfoI	TCCNGGA	1 cut(s)	860
PleI	GAGTC	3 cut(s)	995, 1277, 1633
PluTI	GGCGCC	1 cut(s)	318
PpsI	GAGTC	3 cut(s)	995, 1277, 1633
PpuMI	RGGWCCY	3 cut(s)	943, 1120, 1597
PshAI	GACNNNNGTC	1 cut(s)	773
Psp124BI	GAGCTC	1 cut(s)	1098
Psp5II	RGGWCCY	3 cut(s)	943, 1120, 1597
Psp6I	CCWGG	9 cut(s)	258, 304, 860, 1158, 1190, 1206, 1494, 2266, 2461
PspEI	GGTNACC	1 cut(s)	2480
PspFI	CCCAGC	1 cut(s)	1404
PspGI	CCWGG	9 cut(s)	258, 304, 860, 1158, 1190, 1206, 1494, 2266, 2461
PspPI	GGNCC	9 cut(s)	609, 886, 943, 974, 1120, 1230, 1597, 2260, 2565
PspPPI	RGGWCCY	3 cut(s)	943, 1120, 1597
PsrI	GAACNNNNNNTAC	2 cut(s)	647, 679
PstI	CTGCAG	2 cut(s)	1314, 1984
PsuI	RGATCY	3 cut(s)	1039, 1408, 1856
PsyI	GACNNNGTC	1 cut(s)	122
RsaI	GTAC	3 cut(s)	1554, 1938, 2147
RsaNI	GTAC	3 cut(s)	1553, 1937, 2146
RseI	CAYNNNNRTG	3 cut(s)	638, 1745, 2187
SacI	GAGCTC	1 cut(s)	1098
SalI	GTCGAC	2 cut(s)	206, 266
SaqAI	TTAA	5 cut(s)	366, 651, 2004, 2138, 2211
Sau3AI	GATC	7 cut(s)	63, 1039, 1240, 1408, 1856, 1984, 1996
Sau96I	GGNCC	9 cut(s)	609, 886, 943, 974, 1120, 1230, 1597, 2260, 2565
SchI	GAGTC	3 cut(s)	996, 1277, 1634
SduI	GDGCHC	2 cut(s)	954, 1098
SexAI	ACCWGGT	1 cut(s)	2266
SfaNI	GCATC	4 cut(s)	636, 1765, 1888, 2460
SfcI	CTRYAG	4 cut(s)	1058, 1310, 1980, 2087
SfoI	GGCGCC	1 cut(s)	316
SfuI	TTCGAA	1 cut(s)	2114
SinI	GGWCC	8 cut(s)	886, 943, 974, 1120, 1230, 1597, 2260, 2565
SmiMI	CAYNNNNRTG	3 cut(s)	638, 1745, 2187
SmlI	CTYRAG	3 cut(s)	512, 650, 2303
SmoI	CTYRAG	3 cut(s)	512, 650, 2303
SpeI	ACTAGT	1 cut(s)	521
SseBI	AGGCCT	1 cut(s)	2455
SspDI	GGCGCC	1 cut(s)	314
SspI	AATATT	1 cut(s)	2284
SspMI	CTAG	3 cut(s)	522, 575, 786
SstI	GAGCTC	1 cut(s)	1098
StuI	AGGCCT	1 cut(s)	2455
StyI	CCWWGG	4 cut(s)	328, 627, 903, 1593
TaaI	ACNGT	3 cut(s)	1234, 1735, 1881
TaiI	ACGT	3 cut(s)	126, 562, 1071
TaqI	TCGA	7 cut(s)	207, 267, 615, 985, 1098, 1243, 2114
TaqII	GACCGA	1 cut(s)	213
TauI	GCSGC	5 cut(s)	72, 304, 344, 784, 1904
TfiI	GAWTC	7 cut(s)	149, 243, 383, 1078, 1812, 2233, 2522
Tru1I	TTAA	5 cut(s)	366, 651, 2004, 2138, 2211
Tru9I	TTAA	5 cut(s)	366, 651, 2004, 2138, 2211
TscAI	CASTG	4 cut(s)	65, 1738, 2108, 2374
TseFI	GTSAC	3 cut(s)	1304, 2390, 2480
Tsp45I	GTSAC	3 cut(s)	1304, 2390, 2480
TspDTI	ATGAA	8 cut(s)	518, 656, 1457, 1649, 2030, 2171, 2205, 2246
TspGWI	ACGGA	3 cut(s)	785, 1196, 1749
TspRI	CASTG	4 cut(s)	65, 1738, 2108, 2374
Tth111I	GACNNNGTC	1 cut(s)	122
Van91I	CCANNNNNTGG	1 cut(s)	1853
Vha464I	CTTAAG	1 cut(s)	650
VneI	GTGCAC	1 cut(s)	950
VpaK11BI	GGWCC	8 cut(s)	886, 943, 974, 1120, 1230, 1597, 2260, 2565
XagI	CCTNNNNNAGG	1 cut(s)	1292
XapI	RAATTY	4 cut(s)	376, 1006, 1964, 2066
XceI	RCATGY	2 cut(s)	2354, 2599
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1672
XmiI	GTMKAC	2 cut(s)	207, 267
XmnI	GAANNNNTTC	2 cut(s)	2069, 2166
XspI	CTAG	3 cut(s)	522, 575, 786
ZraI	GACGTC	1 cut(s)	124
Zsp2I	ATGCAT	1 cut(s)	2475

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.