Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0002460

Physical Location & Seq

Genomic Coordinates Forward (+)

54325511 .. 54327928

Gene Structure 2418 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG339500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2418 bp

ATGGAGAGGTTTGGAATGGAGAACGATTACGAGGACGGCCAATGGATCGACGGCGAGTTCTTCTACCGTAAGCGCAAGGAGAGACGCGTACAGTCCAAAGACGATGCCCTGTACGGCTACTCCGATGAAGAAGATGAAGAATATTCAGACGGATCCAGAAAACGCAGGAAGAAAGATCGCTACGTCGACTACACCAAGCCCATTAAATTTGTCTCCACTGAAAAGCTGGACAACAATTCCGATCAGAAGAAAACTGATCACGGCACTCCTGTCATTGGCTTTAACTCGACTGGCATGCGCGATGAAGCTGAAGATGATGATGATGATGATGATGGTGATGGTTTTCTGGCGACAGAGTTTGGGAGGAAGATAAAGGACGGAGCTGAGAGGAGACGTAAGAAGAAGGAAAAGCTGAAATTCAAGAATGAGATTAGAAAATGTGATGGAGATGAAGGAGGCATTGGGGATTTCGAGAAACACACCAAGGGCATAGGAAGGAAGATGTTTGAGAAGATGGGGTACACAGGAGGTGGACTCGGGAAGAATCAGCAAGGCATTGTGAGCCCTATTGAGGTAAAGTTGAGGCCGAAAAATATGGGCATGGGTTTCAATGACTACAAGGAAACAGAAACCAAGCTCCAGAGTTTGCTAGAAGACAAACCTAACAAGCCTACAACCTTAAAACAGAAGCCTTCTTGGAAGAAGAAGAAGAGGACTAGCAAGGAGCATTATCTCTCTGCCGAGGAGTTTATAGCCAAGAAGCAGGAAGAGAGTGATTCCAGTCTTTTTGTTCACAATGTCATTGATATGCGCGGACCTCAAGTTCGAGTGTTGAGCAATTTGGACGATTTGAATGAGCGAGAGGAAGATACAGAAGATGAAGGTGCTTTCATGCCGGAGCTACAGCACAATTTGAAGTTGCTTGTGGACTTGGCTGAGCTCGATATCATGAAACTTGATAGGGATTTGAGGTATGAGAGGGAGGCAGTAATTAGATTGAAGAATGAGAAAGAGAGGTTGGAGCTTGAGGAGGCGAGGCAGAAGCAACATTTGGATAGTATGGAGAGTATTCAGACTGTGTTGGACCAACTGGAGGAAGAGAAATCCATGGGAACATTGACGCTGGACTCGCTTGCAAATAGCTTTGGTGAGCTCAAGAGACGGTATGCAGATGAGTATAAGTTGTGTAAGTTGTCATGCATTGCTTGTTCCTTTGCTCTGCCTTTGTTTATGAGTACTATTCAAGGTTGGGATGCACTGCAGTATCCTTTGTATGGCTTGGATGTCGTGTCCTCCTGGAAGGCTTTGCTTCATGGAGAGGGAAATAAGTGTTCAACATCATCGCCTTATGCGCAGTTGGTTTTTGAGATTGTGAGGATTTCTGGTGCAGATAATTGGCAGGCCAAGGATCCTGAACCCATGCTTCGGTTTTTAGAGGCTTGGGAGAAGCTGCTGCCCCCTTGTGTCCTTAATGACATATTGGATCATTTAGTGTTGCCTAAGTTGAAGGATGCAGTTGGTGTTTGGGAACCATGCCGCGACACTCTTCCCATCCATGTATGGGTGCATCCATGGCTACCATTAATGGGACACAGATTGGAGGAGTTGTATCCGACAATACGTTTCAAGTTGGGTAATGTTCTCAGTGCTTGGCACCCAAGCGACGCGTCTGCTTATGCCATACTCTCGCCGTGGAAGAATGTGTTTGATTCTGCAAGTTGGAAACAACTTATGCATAGGTTCATAGTTCCCAAGTTGCAGCTTGTCCTAGAAGAGTTCCATGTGAACCCTGCAAACCAGAGTGTTGATCAGTTCAATTGGGTCATGAGGTGGGCTTATGTTATTCCCAGTCATATGATAGCCGATCTGATGGTGAAGTTTTTCTTCCCCAAATGGATAACCGTCTTGTTTTACTGGCTGAAATCAAACCCCAACTTTGAAGAGATTTTGCATTGGTACAAGGGTTGGAAAGAAGTCATTCCAGAGGAGCTTCAAGCAAATGAAAGCATCCGCCTTCAGCTCAATTGGGGTCTTGACATGATGAACCGGGCCGTTGAGGGTAGGGACTTGGTCGAACCTTGCCATGTGGAGCAAAGACAATCCGATTTTGAGGCACAGAAAAAAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAAACCAAGCAAACTCCAATGAGACAATGATGAGCTTGAGAGATGTGATCGAAGCCTTTGCGCAGCAGCACAGCTTGCTATTTAAGCCTAAACCCGAGAGGAGGCAAGACGGTCACCAGATATATGCATTGGGTTATGTAAACATCATAGTGGACTCTGTCAATCAAAAGGTGTATGCCCGGAAACCAAGAGAGGAAGCCTGGTCCCTCGTAGACCTTGCAACATTGCTTGACATGCACAATAACTCTCTTGCAAGGAGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000375 GO:0000377 GO:0000390 GO:0000398 GO:0000781 GO:0000784 GO:0003674 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005694 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0022411 GO:0030154 GO:0030198 GO:0031012 GO:0031333 GO:0031974 GO:0031981 GO:0032091 GO:0032502 GO:0032984 GO:0032988 GO:0032991 GO:0034641 GO:0042752 GO:0043021 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043393 GO:0043933 GO:0044087 GO:0044092 GO:0044237 GO:0044238 GO:0044421 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0046483 GO:0048519 GO:0048523 GO:0048869 GO:0050789 GO:0050794 GO:0051098 GO:0051100 GO:0051128 GO:0051129 GO:0065007 GO:0065009 GO:0070013 GO:0070990 GO:0071008 GO:0071013 GO:0071014 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0098687 GO:1901360 GO:1902494 GO:1990446 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-patch GCFC TIP_N

Protein Analysis

805

Amino Acids

93.44

Weight (kDa)

5.76

Isoelectric Point (pI)

45.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIP_N	PF12457	1 - 99	8.1e-13	Tuftelin interacting protein N terminal
G-patch	PF01585	161 - 202	6.1e-13	G-patch domain
GCFC	PF07842	375 - 636	6.4e-85	GC-rich sequence DNA-binding factor-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002460)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17070
fragaria_vesca	FvH4_2g25582 FvH4_5g27320
malus_domestica	MD08G1250100.v1.1 MD15G1445100.v1.1
prunus_persica	Prupe.1G587200_v2.0.a1 Prupe.5G048700_v2.0.a1
pyrus_communis	pycom08g21680 pycom15g39220
rosa_chinensis	RchiOBHm_Chr6g0293801 RchiOBHm_Chr6g0293811 RchiOBHm_Chr7g0218481
rosa_laevigata	RLG00000002370 RLG00000011921 RLG00000012034
rosa_multiflora	Rmu_co8485319.1_g000001 Rmu_sc0001301.1_g000062 Rmu_sc0001301.1_g000063 Rmu_sc0001354.1_g000013 Rmu_sc0023058.1_g000001
rosa_roxburghii	Rroxscaffold_3G00240910 Rroxscaffold_7G00173890 Rroxscaffold_7G00175120
rosa_rugosa	Rorug06G0238200 Rorug07G0177400
rosa_samantha	Rh6AG339400 Rh6AG351600 Rh6BG346700 Rh6BG358400 Rh6CG353500 Rh6CG365600 Rh6DG339500 Rh6DG352200 Rh6DG352300 Rh7AG318900 Rh7BG310800 Rh7CG335800 Rh7DG318000
rosa_wichuraiana	Rw6G029540 Rw6G030580 Rw7G027140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	1353, 2214
AccB1I	GGYRCC	1 cut(s)	1653
AccI	GTMKAC	2 cut(s)	186, 2364
AccII	CGCG	5 cut(s)	87, 300, 813, 1539, 1667
AciI	CCGC	3 cut(s)	813, 1537, 2011
AclWI	GGATC	6 cut(s)	53, 147, 160, 1403, 1416, 1491
AcoI	YGGCCR	1 cut(s)	37
AcsI	RAATTY	2 cut(s)	206, 416
AcuI	CTGAAG	2 cut(s)	330, 2000
AfaI	GTAC	5 cut(s)	90, 113, 521, 1237, 1958
AfiI	CCNNNNNNNGG	8 cut(s)	275, 571, 1093, 1274, 1425, 1561, 1586, 2253
AflIII	ACRYGT	2 cut(s)	85, 1665
AjnI	CCWGG	2 cut(s)	1295, 2351
AjuI	GAANNNNNNNTTGG	6 cut(s)	444, 476, 1001, 1033, 1034, 1066
AloI	GAACNNNNNNTCC	2 cut(s)	807, 839
Alw21I	GWGCWC	2 cut(s)	942, 1155
Alw26I	GTCTC	5 cut(s)	76, 217, 385, 1153, 2168
AlwI	GGATC	6 cut(s)	53, 147, 160, 1403, 1416, 1491
Ama87I	CYCGRG	2 cut(s)	536, 2246
AoxI	GGCC	4 cut(s)	37, 584, 1401, 2049
ApoI	RAATTY	2 cut(s)	206, 416
ArsI	GACNNNNNNTTYG	2 cut(s)	807, 839
AseI	ATTAAT	1 cut(s)	1583
Asp700I	GAANNNNTTC	2 cut(s)	1880, 1977
AspLEI	GCGC	5 cut(s)	75, 300, 813, 1354, 2215
AspS9I	GGNCC	4 cut(s)	815, 1084, 2049, 2355
AsuC2I	CCSGG	2 cut(s)	2048, 2332
AsuHPI	GGTGA	4 cut(s)	347, 1160, 1885, 2258
AvaI	CYCGRG	2 cut(s)	536, 2246
AvaII	GGWCC	3 cut(s)	815, 1084, 2355
BaeI	ACNNNNGTAYC	4 cut(s)	1247, 1280, 1948, 1981
BamHI	GGATCC	2 cut(s)	152, 1408
BanI	GGYRCC	1 cut(s)	1653
BanII	GRGCYC	3 cut(s)	566, 942, 1155
BarI	GAAGNNNNNNTAC	2 cut(s)	503, 535
BbsI	GAAGAC	1 cut(s)	660
Bbv12I	GWGCWC	2 cut(s)	942, 1155
BccI	CCATC	6 cut(s)	326, 332, 437, 508, 1559, 1864
BceAI	ACGGC	6 cut(s)	52, 67, 130, 277, 1675, 2036
BcgI	CGANNNNNNTGC	6 cut(s)	277, 311, 1652, 1686, 2192, 2226
BciT130I	CCWGG	2 cut(s)	1297, 2353
BciVI	GTATCC	2 cut(s)	1275, 1620
BclI	TGATCA	2 cut(s)	256, 1807
BcnI	CCSGG	2 cut(s)	2048, 2332
BcoDI	GTCTC	5 cut(s)	76, 217, 385, 1153, 2168
BfaI	CTAG	3 cut(s)	650, 717, 1769
BfmI	CTRYAG	2 cut(s)	902, 1259
BfuI	GTATCC	2 cut(s)	1275, 1620
BlpI	GCTNAGC	1 cut(s)	936
BmcAI	AGTACT	1 cut(s)	1237
Bme1390I	CCNGG	4 cut(s)	1297, 2048, 2332, 2353
Bme18I	GGWCC	3 cut(s)	815, 1084, 2355
BmeT110I	CYCGRG	2 cut(s)	536, 2246
BmgT120I	GGNCC	4 cut(s)	815, 1084, 2049, 2355
BmiI	GGNNCC	5 cut(s)	154, 1410, 1530, 1655, 2357
BmrFI	CCNGG	4 cut(s)	1297, 2048, 2332, 2353
BmrI	ACTGGG	1 cut(s)	1842
BmsI	GCATC	5 cut(s)	94, 1243, 1501, 1576, 2016
BmuI	ACTGGG	1 cut(s)	1842
BpiI	GAAGAC	1 cut(s)	660
BplI	GAGNNNNNCTC	2 cut(s)	519, 551
BpmI	CTGGAG	2 cut(s)	623, 1112
Bpu1102I	GCTNAGC	1 cut(s)	936
BpuEI	CTTGAG	4 cut(s)	804, 1046, 1139, 2209
BpuMI	CCSGG	2 cut(s)	2048, 2332
BsaJI	CCNNGG	6 cut(s)	483, 741, 1107, 1404, 1571, 1691
BsaXI	ACNNNNNCTCC	4 cut(s)	104, 134, 1592, 1622
Bsc4I	CCNNNNNNNGG	8 cut(s)	275, 571, 1093, 1274, 1425, 1561, 1586, 2253
Bse1I	ACTGG	5 cut(s)	295, 780, 1095, 1848, 1919
Bse3DI	GCAATG	2 cut(s)	1200, 2375
BseBI	CCWGG	2 cut(s)	1297, 2353
BseDI	CCNNGG	6 cut(s)	483, 741, 1107, 1404, 1571, 1691
BseGI	GGATG	6 cut(s)	1258, 1288, 1516, 1551, 1567, 2007
BseLI	CCNNNNNNNGG	8 cut(s)	275, 571, 1093, 1274, 1425, 1561, 1586, 2253
BseMI	GCAATG	2 cut(s)	1200, 2375
BseMII	CTCAG	3 cut(s)	375, 927, 1657
BseNI	ACTGG	5 cut(s)	295, 780, 1095, 1848, 1919
BseRI	GAGGAG	6 cut(s)	403, 758, 1043, 1616, 2000, 2266
BsgI	GTGCAG	1 cut(s)	1407
Bsh1236I	CGCG	5 cut(s)	87, 300, 813, 1539, 1667
BshFI	GGCC	4 cut(s)	39, 586, 1403, 2051
BshNI	GGYRCC	1 cut(s)	1653
BsiHKAI	GWGCWC	2 cut(s)	942, 1155
BsiHKCI	CYCGRG	2 cut(s)	536, 2246
BsiSI	CCGG	3 cut(s)	896, 2047, 2332
BslFI	GGGAC	3 cut(s)	1602, 2078, 2341
BslI	CCNNNNNNNGG	8 cut(s)	275, 571, 1093, 1274, 1425, 1561, 1586, 2253
BsmAI	GTCTC	5 cut(s)	76, 217, 385, 1153, 2168
BsmBI	CGTCTC	3 cut(s)	76, 385, 1153
BsmFI	GGGAC	3 cut(s)	1602, 2078, 2341
BsnI	GGCC	4 cut(s)	39, 586, 1403, 2051
BsoBI	CYCGRG	2 cut(s)	536, 2246
Bsp1286I	GDGCHC	3 cut(s)	566, 942, 1155
Bsp1720I	GCTNAGC	1 cut(s)	936
Bsp19I	CCATGG	2 cut(s)	1107, 1571
BspACI	CCGC	3 cut(s)	813, 1537, 2011
BspANI	GGCC	4 cut(s)	39, 586, 1403, 2051
BspCNI	CTCAG	3 cut(s)	376, 928, 1656
BspFNI	CGCG	5 cut(s)	87, 300, 813, 1539, 1667
BspHI	TCATGA	2 cut(s)	948, 1824
BspLI	GGNNCC	5 cut(s)	154, 1410, 1530, 1655, 2357
BspMAI	CTGCAG	1 cut(s)	1263
BspPI	GGATC	6 cut(s)	53, 147, 160, 1403, 1416, 1491
BspT107I	GGYRCC	1 cut(s)	1653
BsrDI	GCAATG	2 cut(s)	1200, 2375
BsrI	ACTGG	5 cut(s)	295, 780, 1095, 1848, 1919
BssECI	CCNNGG	6 cut(s)	483, 741, 1107, 1404, 1571, 1691
BssT1I	CCWWGG	4 cut(s)	483, 1107, 1404, 1571
Bst2UI	CCWGG	2 cut(s)	1297, 2353
Bst4CI	ACNGT	6 cut(s)	68, 93, 1078, 1164, 1903, 2264
Bst6I	CTCTTC	6 cut(s)	704, 762, 1092, 1551, 1767, 1935
BstAPI	GCANNNNNTGC	1 cut(s)	2227
BstC8I	GCNNGC	4 cut(s)	296, 1134, 1401, 2228
BstDEI	CTNAG	4 cut(s)	384, 936, 1500, 1643
BstDSI	CCRYGG	3 cut(s)	1107, 1571, 1691
BstEII	GGTNACC	1 cut(s)	2264
BstF5I	GGATG	6 cut(s)	1258, 1288, 1516, 1551, 1567, 2007
BstFNI	CGCG	5 cut(s)	87, 300, 813, 1539, 1667
BstHHI	GCGC	5 cut(s)	75, 300, 813, 1354, 2215
BstMAI	GTCTC	5 cut(s)	76, 217, 385, 1153, 2168
BstNI	CCWGG	2 cut(s)	1297, 2353
BstNSI	RCATGY	2 cut(s)	298, 2389
BstPI	GGTNACC	1 cut(s)	2264
BstSCI	CCNGG	4 cut(s)	1295, 2046, 2330, 2351
BstSFI	CTRYAG	2 cut(s)	902, 1259
BstUI	CGCG	5 cut(s)	87, 300, 813, 1539, 1667
BstV2I	GAAGAC	1 cut(s)	660
BstX2I	RGATCY	2 cut(s)	152, 1408
BstYI	RGATCY	2 cut(s)	152, 1408
BsuI	GTATCC	2 cut(s)	1275, 1620
BsuRI	GGCC	4 cut(s)	39, 586, 1403, 2051
BtgI	CCRYGG	3 cut(s)	1107, 1571, 1691
BtgZI	GCGATG	2 cut(s)	315, 1326
BtsCI	GGATG	6 cut(s)	1258, 1288, 1516, 1551, 1567, 2007
BtsI	GCAGTG	1 cut(s)	1256
BtsIMutI	CAGTG	3 cut(s)	216, 1256, 1651
Cac8I	GCNNGC	4 cut(s)	296, 1134, 1401, 2228
CciI	TCATGA	2 cut(s)	948, 1824
CfoI	GCGC	5 cut(s)	75, 300, 813, 1354, 2215
Cfr13I	GGNCC	4 cut(s)	815, 1084, 2049, 2355
CseI	GACGC	4 cut(s)	93, 1129, 1656, 1673
Csp6I	GTAC	5 cut(s)	89, 112, 520, 1236, 1957
CviQI	GTAC	5 cut(s)	89, 112, 520, 1236, 1957
DdeI	CTNAG	4 cut(s)	384, 936, 1500, 1643
EaeI	YGGCCR	1 cut(s)	37
Eam1104I	CTCTTC	6 cut(s)	704, 762, 1092, 1551, 1767, 1935
EarI	CTCTTC	6 cut(s)	704, 762, 1092, 1551, 1767, 1935
EciI	GGCGGA	1 cut(s)	2000
Ecl136II	GAGCTC	2 cut(s)	940, 1153
Eco130I	CCWWGG	4 cut(s)	483, 1107, 1404, 1571
Eco24I	GRGCYC	3 cut(s)	566, 942, 1155
Eco32I	GATATC	1 cut(s)	946
Eco47I	GGWCC	3 cut(s)	815, 1084, 2355
Eco53kI	GAGCTC	2 cut(s)	940, 1153
Eco57I	CTGAAG	2 cut(s)	330, 2000
Eco88I	CYCGRG	2 cut(s)	536, 2246
Eco91I	GGTNACC	1 cut(s)	2264
EcoICRI	GAGCTC	2 cut(s)	940, 1153
EcoO65I	GGTNACC	1 cut(s)	2264
EcoRII	CCWGG	2 cut(s)	1295, 2351
EcoRV	GATATC	1 cut(s)	946
EcoT14I	CCWWGG	4 cut(s)	483, 1107, 1404, 1571
EcoT22I	ATGCAT	3 cut(s)	1202, 1737, 2281
EcoT38I	GRGCYC	3 cut(s)	566, 942, 1155
ErhI	CCWWGG	4 cut(s)	483, 1107, 1404, 1571
Esp3I	CGTCTC	3 cut(s)	76, 385, 1153
FalI	AAGNNNNNCTT	2 cut(s)	1868, 1900
FaqI	GGGAC	3 cut(s)	1602, 2078, 2341
FauNDI	CATATG	1 cut(s)	1854
FbaI	TGATCA	2 cut(s)	256, 1807
FblI	GTMKAC	2 cut(s)	186, 2364
FokI	GGATG	6 cut(s)	1265, 1295, 1523, 1538, 1554, 1994
FriOI	GRGCYC	3 cut(s)	566, 942, 1155
FspBI	CTAG	3 cut(s)	650, 717, 1769
FspI	TGCGCA	2 cut(s)	1353, 2214
GlaI	GCGC	5 cut(s)	74, 299, 812, 1353, 2214
GsuI	CTGGAG	2 cut(s)	623, 1112
HaeIII	GGCC	4 cut(s)	39, 586, 1403, 2051
HapII	CCGG	3 cut(s)	896, 2047, 2332
HgaI	GACGC	4 cut(s)	93, 1129, 1656, 1673
HhaI	GCGC	5 cut(s)	75, 300, 813, 1354, 2215
Hin6I	GCGC	5 cut(s)	73, 298, 811, 1352, 2213
HinP1I	GCGC	5 cut(s)	73, 298, 811, 1352, 2213
HincII	GTYRAC	1 cut(s)	187
HindII	GTYRAC	1 cut(s)	187
HinfI	GANTC	6 cut(s)	534, 544, 776, 1127, 1709, 2306
HpaII	CCGG	3 cut(s)	896, 2047, 2332
HphI	GGTGA	4 cut(s)	347, 1160, 1885, 2258
Hpy166II	GTNNAC	9 cut(s)	187, 522, 533, 793, 928, 1786, 2293, 2305, 2365
Hpy188I	TCNGA	8 cut(s)	124, 148, 241, 246, 1074, 1614, 1869, 2104
Hpy8I	GTNNAC	9 cut(s)	187, 522, 533, 793, 928, 1786, 2293, 2305, 2365
Hpy99I	CGWCG	3 cut(s)	53, 188, 1667
HpyAV	CCTTC	8 cut(s)	397, 446, 489, 702, 875, 1294, 1501, 2024
HpyCH4III	ACNGT	6 cut(s)	68, 93, 1078, 1164, 1903, 2264
HpyCH4IV	ACGT	3 cut(s)	183, 394, 1621
HpyF3I	CTNAG	4 cut(s)	384, 936, 1500, 1643
HpySE526I	ACGT	3 cut(s)	183, 394, 1621
HspAI	GCGC	5 cut(s)	73, 298, 811, 1352, 2213
Ksp22I	TGATCA	2 cut(s)	256, 1807
LmnI	GCTCC	7 cut(s)	380, 642, 724, 898, 1021, 1987, 2089
LweI	GCATC	5 cut(s)	94, 1243, 1501, 1576, 2016
MaeI	CTAG	3 cut(s)	650, 717, 1769
MaeII	ACGT	3 cut(s)	183, 394, 1621
MaeIII	GTNAC	1 cut(s)	2264
MfeI	CAATTG	2 cut(s)	1816, 2023
MflI	RGATCY	2 cut(s)	152, 1408
MhlI	GDGCHC	3 cut(s)	566, 942, 1155
MluCI	AATT	9 cut(s)	206, 235, 416, 838, 910, 990, 1393, 1816, 2023
MluI	ACGCGT	2 cut(s)	85, 1665
MlyI	GAGTC	3 cut(s)	528, 1121, 2300
MmeI	TCCRAC	5 cut(s)	999, 1062, 1637, 1700, 1946
Mph1103I	ATGCAT	3 cut(s)	1202, 1737, 2281
MroXI	GAANNNNTTC	2 cut(s)	1880, 1977
MseI	TTAA	6 cut(s)	204, 282, 680, 1470, 1583, 2235
MslI	CAYNNNNRTG	2 cut(s)	806, 2300
MspI	CCGG	3 cut(s)	896, 2047, 2332
MspR9I	CCNGG	4 cut(s)	1297, 2048, 2332, 2353
MunI	CAATTG	2 cut(s)	1816, 2023
MvaI	CCWGG	2 cut(s)	1297, 2353
MvnI	CGCG	5 cut(s)	87, 300, 813, 1539, 1667
NciI	CCSGG	2 cut(s)	2048, 2332
NcoI	CCATGG	2 cut(s)	1107, 1571
NdeI	CATATG	1 cut(s)	1854
NlaIV	GGNNCC	5 cut(s)	154, 1410, 1530, 1655, 2357
NmeAIII	GCCGAG	1 cut(s)	766
NmuCI	GTSAC	1 cut(s)	2264
NsbI	TGCGCA	2 cut(s)	1353, 2214
NsiI	ATGCAT	3 cut(s)	1202, 1737, 2281
NspI	RCATGY	2 cut(s)	298, 2389
PaeI	GCATGC	1 cut(s)	298
PagI	TCATGA	2 cut(s)	948, 1824
PcsI	WCGNNNNNNNCGW	1 cut(s)	120
PdmI	GAANNNNTTC	2 cut(s)	1880, 1977
PfeI	GAWTC	3 cut(s)	544, 776, 1709
PflFI	GACNNNGTC	2 cut(s)	2069, 2309
PfoI	TCCNGGA	1 cut(s)	1295
PleI	GAGTC	3 cut(s)	528, 1121, 2300
PpsI	GAGTC	3 cut(s)	528, 1121, 2300
PshBI	ATTAAT	1 cut(s)	1583
Psp124BI	GAGCTC	2 cut(s)	942, 1155
Psp6I	CCWGG	2 cut(s)	1295, 2351
PspEI	GGTNACC	1 cut(s)	2264
PspGI	CCWGG	2 cut(s)	1295, 2351
PspN4I	GGNNCC	5 cut(s)	154, 1410, 1530, 1655, 2357
PspPI	GGNCC	4 cut(s)	815, 1084, 2049, 2355
PstI	CTGCAG	1 cut(s)	1263
PsuI	RGATCY	2 cut(s)	152, 1408
PsyI	GACNNNGTC	2 cut(s)	2069, 2309
RsaI	GTAC	5 cut(s)	90, 113, 521, 1237, 1958
RsaNI	GTAC	5 cut(s)	89, 112, 520, 1236, 1957
RseI	CAYNNNNRTG	2 cut(s)	806, 2300
SacI	GAGCTC	2 cut(s)	942, 1155
SalI	GTCGAC	1 cut(s)	185
SaqAI	TTAA	6 cut(s)	204, 282, 680, 1470, 1583, 2235
Sau96I	GGNCC	4 cut(s)	815, 1084, 2049, 2355
ScaI	AGTACT	1 cut(s)	1237
SchI	GAGTC	3 cut(s)	528, 1121, 2300
ScrFI	CCNGG	4 cut(s)	1297, 2048, 2332, 2353
SduI	GDGCHC	3 cut(s)	566, 942, 1155
SfaNI	GCATC	5 cut(s)	94, 1243, 1501, 1576, 2016
SfcI	CTRYAG	2 cut(s)	902, 1259
SinI	GGWCC	3 cut(s)	815, 1084, 2355
SmiMI	CAYNNNNRTG	2 cut(s)	806, 2300
SmlI	CTYRAG	4 cut(s)	819, 1025, 1154, 2188
SmoI	CTYRAG	4 cut(s)	819, 1025, 1154, 2188
SphI	GCATGC	1 cut(s)	298
Sse9I	AATT	9 cut(s)	206, 235, 416, 838, 910, 990, 1393, 1816, 2023
SsiI	CCGC	3 cut(s)	813, 1537, 2011
SspI	AATATT	1 cut(s)	143
SspMI	CTAG	3 cut(s)	650, 717, 1769
SstI	GAGCTC	2 cut(s)	942, 1155
StyD4I	CCNGG	4 cut(s)	1295, 2046, 2330, 2351
StyI	CCWWGG	4 cut(s)	483, 1107, 1404, 1571
TaaI	ACNGT	6 cut(s)	68, 93, 1078, 1164, 1903, 2264
TaiI	ACGT	3 cut(s)	186, 397, 1624
TaqI	TCGA	8 cut(s)	48, 186, 287, 471, 826, 942, 2073, 2202
TasI	AATT	9 cut(s)	206, 235, 416, 838, 910, 990, 1393, 1816, 2023
TatI	WGTACW	1 cut(s)	1235
TauI	GCSGC	1 cut(s)	1539
TfiI	GAWTC	3 cut(s)	544, 776, 1709
Tru1I	TTAA	6 cut(s)	204, 282, 680, 1470, 1583, 2235
Tru9I	TTAA	6 cut(s)	204, 282, 680, 1470, 1583, 2235
TscAI	CASTG	3 cut(s)	223, 1263, 1651
TseFI	GTSAC	1 cut(s)	2264
Tsp45I	GTSAC	1 cut(s)	2264
TspGWI	ACGGA	2 cut(s)	165, 393
TspRI	CASTG	3 cut(s)	223, 1263, 1651
Tth111I	GACNNNGTC	2 cut(s)	2069, 2309
VpaK11BI	GGWCC	3 cut(s)	815, 1084, 2355
VspI	ATTAAT	1 cut(s)	1583
XapI	RAATTY	2 cut(s)	206, 416
XceI	RCATGY	2 cut(s)	298, 2389
XcmI	CCANNNNNNNNNTGG	1 cut(s)	223
XmiI	GTMKAC	2 cut(s)	186, 2364
XmnI	GAANNNNTTC	2 cut(s)	1880, 1977
XspI	CTAG	3 cut(s)	650, 717, 1769
ZrmI	AGTACT	1 cut(s)	1237
Zsp2I	ATGCAT	3 cut(s)	1202, 1737, 2281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.