MD09G1015300.v1.1

Phosducin-like protein 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002093
Physical Location & Seq
Reverse (-)
950590 .. 952418
1829 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1015300.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGCGGACTATCACTTTGTGTACAAGGATGTGGAGGGAGCATCGACCCAGTGGGACGACATACAGAGAAAGCTCGGGAACCTTCCGGCGAAGCCACCGCCGTTCAAGCCGCCGAAGTTCACACCAGCTCAGGACGAAGCCTCCCTTCCCAAAGACAAGTCCTGGATCGATGAAAAATCCGAACAGGACCTCGAAGACCTCGAGGACAATCCCGATCTCGATGACGATCGCTTCCTCGAAGAATACAGGAAGAAGAGGCTGGCGGAGCTGAGAGAAGCAGCCAAAGTTGCGAGGTTCGGATCGGTGGTGCCGATTTCGGGATCGGATTTCGTGCGGGAGGTGTCGCAGGCGCCGCCGGATGTGTGGGTGGTGGTGATTCTGTACAAAGAAGGAATCCCCGAGTGCGGGGTTTTGATGCAGTGTTTGGATGATTTGGCGACCAAATATCCGGCTACGAAATTCGTCAAGATAATATCGACGGACTGCATTCCGAACTACCCTGATCGGAATGTTCCGACTGTTTTAGTGTACAACAGTGGGAATGTCAAAGCTAATTACGTCGGCTTGCAGAGCTTCGGCCGCAGATGCACACCTGAAGGTGTGGCATTACTTCTGTGCCAATCAGATCCTGTACTTAATGATGGCCTGAGCGGAAATGATCGGTCGAGGCAAGCTGTGATCGACGGAGTTCGCAAGAGGTTTATAGAGAAGGTTGTGACAGAGCATGAGGATGTTGACGATGGATCCTCGAGCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phosducin

Protein Analysis

253

Amino Acids

28.22

Weight (kDa)

4.73

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phosducin PF02114 71 - 188 1.9e-15 Phosducin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 200
AccB1I GGYRCC 2 cut(s) 307, 349
AccBSI CCGCTC 1 cut(s) 651
AciI CCGC 9 cut(s) 5, 98, 110, 263, 334, 353, 405, 580, 651
AclWI GGATC 6 cut(s) 173, 307, 328, 620, 738, 751
AcoI YGGCCR 1 cut(s) 577
AcsI RAATTY 1 cut(s) 458
AcuI CTGAAG 1 cut(s) 615
AcyI GRCGYC 1 cut(s) 350
AdeI CACNNNGTG 1 cut(s) 19
AfaI GTAC 4 cut(s) 23, 383, 530, 633
AfiI CCNNNNNNNGG 3 cut(s) 317, 404, 405
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 1 cut(s) 161
AluBI AGCT 6 cut(s) 73, 128, 268, 551, 573, 674
AluI AGCT 6 cut(s) 73, 128, 268, 551, 573, 674
AlwI GGATC 6 cut(s) 173, 307, 328, 620, 738, 751
AlwNI CAGNNNCTG 1 cut(s) 629
Ama87I CYCGRG 4 cut(s) 74, 200, 398, 748
AoxI GGCC 2 cut(s) 577, 643
ApeKI GCWGC 1 cut(s) 278
ApoI RAATTY 1 cut(s) 458
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspLEI GCGC 1 cut(s) 352
AspS9I GGNCC 1 cut(s) 187
AsuHPI GGTGA 1 cut(s) 385
AvaI CYCGRG 4 cut(s) 74, 200, 398, 748
AvaII GGWCC 1 cut(s) 187
BamHI GGATCC 1 cut(s) 743
BanI GGYRCC 2 cut(s) 307, 349
BbsI GAAGAC 1 cut(s) 201
BbvI GCAGC 1 cut(s) 290
BccI CCATC 2 cut(s) 635, 734
BceAI ACGGC 1 cut(s) 85
BciT130I CCWGG 1 cut(s) 163
BfoI RGCGCY 1 cut(s) 353
BisI GCNGC 4 cut(s) 110, 279, 353, 580
BlsI GCNGC 4 cut(s) 111, 280, 354, 581
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 1 cut(s) 187
BmeT110I CYCGRG 4 cut(s) 74, 200, 398, 748
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 4 cut(s) 80, 309, 351, 745
BmrFI CCNGG 1 cut(s) 163
BmrI ACTGGG 1 cut(s) 43
BmsI GCATC 3 cut(s) 50, 405, 575
BmuI ACTGGG 1 cut(s) 43
BpiI GAAGAC 1 cut(s) 201
Bpu10I CCTNAGC 2 cut(s) 129, 647
Bsa29I ATCGAT 1 cut(s) 168
BsaBI GATNNNNATC 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 350
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 3 cut(s) 317, 404, 405
Bse1I ACTGG 1 cut(s) 49
Bse8I GATNNNNATC 1 cut(s) 225
BseBI CCWGG 1 cut(s) 163
BseCI ATCGAT 1 cut(s) 168
BseGI GGATG 4 cut(s) 34, 364, 433, 736
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 3 cut(s) 317, 404, 405
BseMII CTCAG 3 cut(s) 143, 260, 638
BseNI ACTGG 1 cut(s) 49
BseX3I CGGCCG 1 cut(s) 577
BseXI GCAGC 1 cut(s) 290
Bsh1285I CGRYCG 3 cut(s) 229, 580, 665
BshFI GGCC 2 cut(s) 579, 645
BshNI GGYRCC 2 cut(s) 307, 349
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 3 cut(s) 229, 580, 665
BsiHKCI CYCGRG 4 cut(s) 74, 200, 398, 748
BsiSI CCGG 3 cut(s) 86, 356, 449
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 3 cut(s) 317, 404, 405
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 2 cut(s) 579, 645
BsoBI CYCGRG 4 cut(s) 74, 200, 398, 748
Bsp1407I TGTACA 3 cut(s) 21, 381, 528
BspACI CCGC 9 cut(s) 5, 98, 110, 263, 334, 353, 405, 580, 651
BspANI GGCC 2 cut(s) 579, 645
BspCNI CTCAG 3 cut(s) 142, 261, 639
BspDI ATCGAT 1 cut(s) 168
BspLI GGNNCC 4 cut(s) 80, 309, 351, 745
BspPI GGATC 6 cut(s) 173, 307, 328, 620, 738, 751
BspT107I GGYRCC 2 cut(s) 307, 349
BsrBI CCGCTC 1 cut(s) 651
BsrGI TGTACA 3 cut(s) 21, 381, 528
BsrI ACTGG 1 cut(s) 49
BssNI GRCGYC 1 cut(s) 350
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 2 cut(s) 520, 536
Bst6I CTCTTC 1 cut(s) 248
BstACI GRCGYC 1 cut(s) 350
BstAUI TGTACA 3 cut(s) 21, 381, 528
BstC8I GCNNGC 4 cut(s) 261, 348, 566, 672
BstDEI CTNAG 3 cut(s) 129, 269, 647
BstF5I GGATG 4 cut(s) 34, 364, 433, 736
BstH2I RGCGCY 1 cut(s) 353
BstHHI GCGC 1 cut(s) 352
BstMCI CGRYCG 3 cut(s) 229, 580, 665
BstMWI GCNNNNNNNGC 7 cut(s) 106, 265, 287, 352, 570, 579, 585
BstNI CCWGG 1 cut(s) 163
BstSCI CCNGG 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 290
BstV2I GAAGAC 1 cut(s) 201
BstX2I RGATCY 2 cut(s) 625, 743
BstYI RGATCY 2 cut(s) 625, 743
BstZI CGGCCG 1 cut(s) 577
Bsu15I ATCGAT 1 cut(s) 168
BsuRI GGCC 2 cut(s) 579, 645
BsuTUI ATCGAT 1 cut(s) 168
BtsCI GGATG 4 cut(s) 34, 364, 433, 736
BtsI GCAGTG 1 cut(s) 425
BtsIMutI CAGTG 3 cut(s) 56, 425, 541
Cac8I GCNNGC 4 cut(s) 261, 348, 566, 672
CaiI CAGNNNCTG 1 cut(s) 629
CfoI GCGC 1 cut(s) 352
Cfr13I GGNCC 1 cut(s) 187
ClaI ATCGAT 1 cut(s) 168
Csp6I GTAC 4 cut(s) 22, 382, 529, 632
CviAII CATG 1 cut(s) 725
CviQI GTAC 4 cut(s) 22, 382, 529, 632
DdeI CTNAG 3 cut(s) 129, 269, 647
DinI GGCGCC 1 cut(s) 351
DraIII CACNNNGTG 1 cut(s) 19
EaeI YGGCCR 1 cut(s) 577
EagI CGGCCG 1 cut(s) 577
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
EciI GGCGGA 2 cut(s) 20, 278
EclXI CGGCCG 1 cut(s) 577
Eco47I GGWCC 1 cut(s) 187
Eco52I CGGCCG 1 cut(s) 577
Eco57I CTGAAG 1 cut(s) 615
Eco88I CYCGRG 4 cut(s) 74, 200, 398, 748
EcoO109I RGGNCCY 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 161
EgeI GGCGCC 1 cut(s) 351
EheI GGCGCC 1 cut(s) 351
FaeI CATG 1 cut(s) 728
FaiI YATR 3 cut(s) 62, 704, 726
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FaqI GGGAC 1 cut(s) 68
FatI CATG 1 cut(s) 724
FauI CCCGC 2 cut(s) 327, 398
Fnu4HI GCNGC 4 cut(s) 110, 279, 353, 580
FokI GGATG 4 cut(s) 41, 371, 440, 743
Fsp4HI GCNGC 4 cut(s) 110, 279, 353, 580
GlaI GCGC 1 cut(s) 351
GluI GCNGC 4 cut(s) 110, 279, 353, 580
HaeII RGCGCY 1 cut(s) 353
HaeIII GGCC 2 cut(s) 579, 645
HapII CCGG 3 cut(s) 86, 356, 449
HhaI GCGC 1 cut(s) 352
Hin1I GRCGYC 1 cut(s) 350
Hin1II CATG 1 cut(s) 728
Hin6I GCGC 1 cut(s) 350
HinP1I GCGC 1 cut(s) 350
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HinfI GANTC 2 cut(s) 376, 393
HpaII CCGG 3 cut(s) 86, 356, 449
HphI GGTGA 1 cut(s) 385
Hpy166II GTNNAC 4 cut(s) 22, 120, 529, 736
Hpy188I TCNGA 7 cut(s) 181, 299, 325, 492, 507, 516, 625
Hpy188III TCNNGA 6 cut(s) 76, 131, 212, 218, 318, 466
Hpy8I GTNNAC 4 cut(s) 22, 120, 529, 736
Hpy99I CGWCG 3 cut(s) 481, 563, 686
HpyAV CCTTC 5 cut(s) 92, 155, 383, 590, 703
HpyCH4III ACNGT 2 cut(s) 520, 536
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 4 cut(s) 418, 486, 568, 588
HpyF10VI GCNNNNNNNGC 7 cut(s) 106, 265, 287, 352, 570, 579, 585
HpyF3I CTNAG 3 cut(s) 129, 269, 647
HpySE526I ACGT 1 cut(s) 558
Hsp92I GRCGYC 1 cut(s) 350
Hsp92II CATG 1 cut(s) 728
HspAI GCGC 1 cut(s) 350
KasI GGCGCC 1 cut(s) 349
LmnI GCTCC 2 cut(s) 38, 265
Lsp1109I GCAGC 1 cut(s) 290
LweI GCATC 3 cut(s) 50, 405, 575
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 1 cut(s) 715
MbiI CCGCTC 1 cut(s) 651
MboII GAAGA 4 cut(s) 206, 251, 262, 265
MflI RGATCY 2 cut(s) 625, 743
MluCI AATT 2 cut(s) 458, 553
Mly113I GGCGCC 1 cut(s) 350
MmeI TCCRAC 1 cut(s) 539
MseI TTAA 1 cut(s) 636
MslI CAYNNNNRTG 1 cut(s) 729
MspI CCGG 3 cut(s) 86, 356, 449
MspR9I CCNGG 1 cut(s) 163
Mva1269I GAATGC 1 cut(s) 486
MvaI CCWGG 1 cut(s) 163
MwoI GCNNNNNNNGC 7 cut(s) 106, 265, 287, 352, 570, 579, 585
NarI GGCGCC 1 cut(s) 350
NlaIII CATG 1 cut(s) 728
NlaIV GGNNCC 4 cut(s) 80, 309, 351, 745
NmuCI GTSAC 1 cut(s) 715
PaeR7I CTCGAG 2 cut(s) 200, 748
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 308
PctI GAATGC 1 cut(s) 486
PfeI GAWTC 2 cut(s) 376, 393
PfoI TCCNGGA 1 cut(s) 161
PkrI GCNGC 4 cut(s) 111, 280, 354, 581
Ple19I CGATCG 1 cut(s) 229
PluTI GGCGCC 1 cut(s) 353
PpuMI RGGWCCY 1 cut(s) 187
Psp5II RGGWCCY 1 cut(s) 187
Psp6I CCWGG 1 cut(s) 161
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 4 cut(s) 80, 309, 351, 745
PspPI GGNCC 1 cut(s) 187
PspPPI RGGWCCY 1 cut(s) 187
PspXI VCTCGAGB 2 cut(s) 200, 748
PstNI CAGNNNCTG 1 cut(s) 629
PsuI RGATCY 2 cut(s) 625, 743
PvuI CGATCG 1 cut(s) 229
RsaI GTAC 4 cut(s) 23, 383, 530, 633
RsaNI GTAC 4 cut(s) 22, 382, 529, 632
RseI CAYNNNNRTG 1 cut(s) 729
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 4 cut(s) 110, 279, 353, 580
Sau96I GGNCC 1 cut(s) 187
ScrFI CCNGG 1 cut(s) 163
SfaNI GCATC 3 cut(s) 50, 405, 575
SfoI GGCGCC 1 cut(s) 351
Sfr274I CTCGAG 2 cut(s) 200, 748
SinI GGWCC 1 cut(s) 187
SlaI CTCGAG 2 cut(s) 200, 748
SmiMI CAYNNNNRTG 1 cut(s) 729
SmlI CTYRAG 2 cut(s) 200, 748
SmoI CTYRAG 2 cut(s) 200, 748
Sse9I AATT 2 cut(s) 458, 553
SsiI CCGC 9 cut(s) 5, 98, 110, 263, 334, 353, 405, 580, 651
SspDI GGCGCC 1 cut(s) 349
StyD4I CCNGG 1 cut(s) 161
TaaI ACNGT 2 cut(s) 520, 536
TaiI ACGT 1 cut(s) 561
TaqII GACCGA 1 cut(s) 651
TasI AATT 2 cut(s) 458, 553
TatI WGTACW 4 cut(s) 21, 381, 528, 631
TauI GCSGC 3 cut(s) 112, 355, 582
TfiI GAWTC 2 cut(s) 376, 393
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 3 cut(s) 56, 425, 541
TseFI GTSAC 1 cut(s) 715
TseI GCWGC 1 cut(s) 278
Tsp45I GTSAC 1 cut(s) 715
TspDTI ATGAA 1 cut(s) 186
TspGWI ACGGA 2 cut(s) 494, 699
TspRI CASTG 3 cut(s) 56, 425, 541
VpaK11BI GGWCC 1 cut(s) 187
XapI RAATTY 1 cut(s) 458
XhoI CTCGAG 2 cut(s) 200, 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.