Rh2AG497000

Phosducin-like protein 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002093
Physical Location & Seq
Forward (+)
71835799 .. 71836640
842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG497000.1

Sequence Viewer

Length: 570 bp
ATGGACACAGACACTCTCGAATATATTGTTGTATCTTCAAGGAGGATGGGGGACTATCACTATATCTACAAGGACTTGGACGGAGCCTCCACCCAGTGGAACGATAATCAGGCAAAGCTTGGCAATATTCCGCCCAAGAAACCAGCGTTTAAGCCTCCTCCCTTCACTCCGGCCGACGACGAAGCCTCTCTTCCTAAAGACAAGTCTTGGATTGATGACAAGACCCAACAGGACCTCGAAGACGATCCCGATCTGGGTGACGATCCCGATCTGGACGACGATCTCTTCCTCGAGGAATACAGGAGGAAGAGGTTGTTTGAATTGAGAGAAGCGGCTGAGGTTGCAAGTTTTGGATCGGTGGTTCCAATTTCGGGATCAGATTTTGTGCGTGAGGTTTCTCAAGCTCCACTTGATGTTTGGGTGGTGGTGATTCTCTACAAAGAAGGAATTGCAGAATGTGGATTGTTGATGCAGTGTCTCAAAGATTTGGCAACCAAGTACCCAGCTACCAAATTTGTGAAAATAATATCCACGATTTGGGGGACGATAAGATGGAGTTGCTGGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phosducin

Protein Analysis

189

Amino Acids

21.53

Weight (kDa)

4.47

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phosducin PF02114 89 - 181 5.2e-10 Phosducin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 290
AccB7I CCANNNNNTGG 2 cut(s) 96, 537
AciI CCGC 2 cut(s) 131, 332
AclWI GGATC 4 cut(s) 239, 257, 361, 382
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 512
AdeI CACNNNGTG 1 cut(s) 96
AfaI GTAC 1 cut(s) 500
AfiI CCNNNNNNNGG 4 cut(s) 96, 254, 371, 537
AgsI TTSAA 2 cut(s) 39, 320
AloI GAACNNNNNNTCC 2 cut(s) 345, 377
AluBI AGCT 3 cut(s) 118, 404, 506
AluI AGCT 3 cut(s) 118, 404, 506
Alw26I GTCTC 1 cut(s) 482
AlwI GGATC 4 cut(s) 239, 257, 361, 382
Ama87I CYCGRG 1 cut(s) 290
AoxI GGCC 1 cut(s) 171
ApoI RAATTY 1 cut(s) 512
AspS9I GGNCC 1 cut(s) 232
AsuHPI GGTGA 2 cut(s) 269, 439
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 1 cut(s) 232
BbsI GAAGAC 1 cut(s) 246
BbvCI CCTCAGC 1 cut(s) 336
BccI CCATC 2 cut(s) 40, 546
BcoDI GTCTC 1 cut(s) 482
BisI GCNGC 1 cut(s) 333
BlsI GCNGC 1 cut(s) 334
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 290
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 2 cut(s) 85, 363
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 1 cut(s) 459
BmuI ACTGGG 1 cut(s) 88
BpiI GAAGAC 1 cut(s) 246
Bpu10I CCTNAGC 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 384
BsaBI GATNNNNATC 2 cut(s) 249, 267
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 4 cut(s) 96, 254, 371, 537
Bse1I ACTGG 1 cut(s) 94
Bse8I GATNNNNATC 2 cut(s) 249, 267
BseGI GGATG 1 cut(s) 51
BseJI GATNNNNATC 2 cut(s) 249, 267
BseLI CCNNNNNNNGG 4 cut(s) 96, 254, 371, 537
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 1 cut(s) 147
BseX3I CGGCCG 1 cut(s) 171
BseYI CCCAGC 1 cut(s) 502
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 173
BsiEI CGRYCG 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 1 cut(s) 170
BslFI GGGAC 2 cut(s) 65, 556
BslI CCNNNNNNNGG 4 cut(s) 96, 254, 371, 537
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 2 cut(s) 65, 556
BsnI GGCC 1 cut(s) 173
BsoBI CYCGRG 1 cut(s) 290
Bsp143I GATC 7 cut(s) 244, 250, 262, 268, 280, 353, 374
BspACI CCGC 2 cut(s) 131, 332
BspANI GGCC 1 cut(s) 173
BspCNI CTCAG 1 cut(s) 328
BspLI GGNNCC 2 cut(s) 85, 363
BspPI GGATC 4 cut(s) 239, 257, 361, 382
BsrI ACTGG 1 cut(s) 94
BssMI GATC 7 cut(s) 244, 250, 262, 268, 280, 353, 374
Bst6I CTCTTC 3 cut(s) 195, 290, 302
BstDEI CTNAG 1 cut(s) 336
BstF5I GGATG 1 cut(s) 51
BstKTI GATC 7 cut(s) 247, 253, 265, 271, 283, 356, 377
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 7 cut(s) 244, 250, 262, 268, 280, 353, 374
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstV2I GAAGAC 1 cut(s) 246
BstZI CGGCCG 1 cut(s) 171
BsuRI GGCC 1 cut(s) 173
BtsCI GGATG 1 cut(s) 51
BtsI GCAGTG 1 cut(s) 479
BtsIMutI CAGTG 2 cut(s) 101, 479
Cfr13I GGNCC 1 cut(s) 232
Csp6I GTAC 1 cut(s) 499
CviJI RGCY 8 cut(s) 86, 118, 154, 173, 185, 335, 404, 506
CviKI_1 RGCY 8 cut(s) 86, 118, 154, 173, 185, 335, 404, 506
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 1 cut(s) 336
DpnI GATC 7 cut(s) 246, 252, 264, 270, 282, 355, 376
DpnII GATC 7 cut(s) 244, 250, 262, 268, 280, 353, 374
DraIII CACNNNGTG 1 cut(s) 96
EaeI YGGCCR 1 cut(s) 171
EagI CGGCCG 1 cut(s) 171
Eam1104I CTCTTC 3 cut(s) 195, 290, 302
EarI CTCTTC 3 cut(s) 195, 290, 302
EciI GGCGGA 1 cut(s) 120
EclXI CGGCCG 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 232
Eco52I CGGCCG 1 cut(s) 171
Eco88I CYCGRG 1 cut(s) 290
EcoO109I RGGNCCY 1 cut(s) 232
FaiI YATR 2 cut(s) 24, 63
FalI AAGNNNNNCTT 4 cut(s) 174, 206, 393, 425
FaqI GGGAC 2 cut(s) 65, 556
Fnu4HI GCNGC 1 cut(s) 333
FokI GGATG 1 cut(s) 58
Fsp4HI GCNGC 1 cut(s) 333
GluI GCNGC 1 cut(s) 333
GsaI CCCAGC 1 cut(s) 506
HaeIII GGCC 1 cut(s) 173
HapII CCGG 1 cut(s) 170
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 1 cut(s) 430
HpaII CCGG 1 cut(s) 170
HphI GGTGA 2 cut(s) 269, 439
Hpy188I TCNGA 1 cut(s) 379
Hpy188III TCNNGA 5 cut(s) 17, 248, 266, 272, 372
Hpy99I CGWCG 3 cut(s) 179, 182, 281
HpyAV CCTTC 2 cut(s) 172, 437
HpyCH4V TGCA 3 cut(s) 344, 452, 472
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 1 cut(s) 336
Kzo9I GATC 7 cut(s) 244, 250, 262, 268, 280, 353, 374
LmnI GCTCC 2 cut(s) 83, 409
LweI GCATC 1 cut(s) 459
MaeIII GTNAC 1 cut(s) 257
MalI GATC 7 cut(s) 246, 252, 264, 270, 282, 355, 376
MboI GATC 7 cut(s) 244, 250, 262, 268, 280, 353, 374
MboII GAAGA 5 cut(s) 27, 182, 251, 277, 319
MluCI AATT 4 cut(s) 320, 366, 447, 512
MseI TTAA 1 cut(s) 150
MspI CCGG 1 cut(s) 170
MwoI GCNNNNNNNGC 1 cut(s) 341
NdeII GATC 7 cut(s) 244, 250, 262, 268, 280, 353, 374
NlaIV GGNNCC 2 cut(s) 85, 363
NmuCI GTSAC 1 cut(s) 257
PaeR7I CTCGAG 1 cut(s) 290
PfeI GAWTC 1 cut(s) 430
PflMI CCANNNNNTGG 2 cut(s) 96, 537
PkrI GCNGC 1 cut(s) 334
PpuMI RGGWCCY 1 cut(s) 232
Psp5II RGGWCCY 1 cut(s) 232
PspFI CCCAGC 1 cut(s) 502
PspN4I GGNNCC 2 cut(s) 85, 363
PspPI GGNCC 1 cut(s) 232
PspPPI RGGWCCY 1 cut(s) 232
PspXI VCTCGAGB 1 cut(s) 290
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 1 cut(s) 333
Sau3AI GATC 7 cut(s) 244, 250, 262, 268, 280, 353, 374
Sau96I GGNCC 1 cut(s) 232
SetI ASST 7 cut(s) 120, 237, 314, 342, 396, 406, 508
SfaNI GCATC 1 cut(s) 459
Sfr274I CTCGAG 1 cut(s) 290
SinI GGWCC 1 cut(s) 232
SlaI CTCGAG 1 cut(s) 290
SmlI CTYRAG 2 cut(s) 290, 399
SmoI CTYRAG 2 cut(s) 290, 399
Sse9I AATT 4 cut(s) 320, 366, 447, 512
SsiI CCGC 2 cut(s) 131, 332
SspI AATATT 1 cut(s) 127
TaqI TCGA 3 cut(s) 18, 237, 291
TasI AATT 4 cut(s) 320, 366, 447, 512
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 1 cut(s) 430
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TscAI CASTG 2 cut(s) 101, 479
TseFI GTSAC 1 cut(s) 257
Tsp45I GTSAC 1 cut(s) 257
TspGWI ACGGA 1 cut(s) 96
TspRI CASTG 2 cut(s) 101, 479
Van91I CCANNNNNTGG 2 cut(s) 96, 537
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 1 cut(s) 512
XcmI CCANNNNNNNNNTGG 1 cut(s) 414
XhoI CTCGAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.