MD17G1015400.v1.1

Phosducin-like protein 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002093
Physical Location & Seq
Reverse (-)
1260488 .. 1262310
1823 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1015400.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGCGGACTATCACTTTGTGTACAAGGACTTGGAGGGAGCATCGACTCAGTGGGACGACATACAGAGAAAGCTCGGGAACCTTCCCGAGAAGCCACCGCCGTTCAAGCCGCCGAAGTTCACACCGGCTCAGGACGAAGCCTCCCTTCCCAAAGACAAGTCCTGGATCGATGAGAAATCCGAGCAGGACCTCGAAGACCTCGAGGACAATCCCGATCTCGACGACGATCGCTTCCTCGAAGAATACAGGAAGAAGAGGCTGGCGGAGCTGAGAGAAGCAGCCAAAGTTGCAAGGTTCGGATCGGTGGTGCCGATTTCGGGATCGGATTTCGTGCGGGAGGTGTCTCAGGCGCCGCCGGATGTCTGGGTGGTTGTGATTTTGTACAAAGAAGGAATCGCCGAGTGCGGGGTTTTGATGCAGTGTATGGAAGATTTGGCGACGAAATATCCCGCTACAAAATTCGTCAAGATAATATCGACGGACTGCATTCCGAACTACCCTGATCGGAATGTTCCGACTGTTTTAGTATACAACAGTGGGAATGTCAAAGCTAACTACGTCGGCTTGCAGAGCTTTGGCCGCAGATGCACACCTGAAGGTGTGGCATTACTTCTGTGCCAATCAGATCCTGTACTTAATGATGGCCTGAGTGGAAATGATCGGTCGAGGCAAGCTGTGATCGATGGAGTTCGCAGGAGGTTTATAGAGAAGGTTGTGACAGAGCATGAAGACGTTGATGATGGGTCCTCGAGCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phosducin

Protein Analysis

253

Amino Acids

28.33

Weight (kDa)

4.7

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phosducin PF02114 71 - 188 2.3e-15 Phosducin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 200
AccB1I GGYRCC 2 cut(s) 307, 349
AccI GTMKAC 1 cut(s) 528
AciI CCGC 9 cut(s) 5, 98, 110, 263, 334, 353, 405, 450, 580
AclWI GGATC 4 cut(s) 173, 307, 328, 620
AcoI YGGCCR 1 cut(s) 577
AcsI RAATTY 1 cut(s) 458
AcuI CTGAAG 1 cut(s) 615
AcyI GRCGYC 1 cut(s) 350
AdeI CACNNNGTG 1 cut(s) 19
AfaI GTAC 3 cut(s) 23, 383, 633
AfiI CCNNNNNNNGG 2 cut(s) 317, 405
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 1 cut(s) 161
AluBI AGCT 5 cut(s) 73, 268, 551, 573, 674
AluI AGCT 5 cut(s) 73, 268, 551, 573, 674
Alw26I GTCTC 1 cut(s) 348
AlwI GGATC 4 cut(s) 173, 307, 328, 620
AlwNI CAGNNNCTG 1 cut(s) 629
Ama87I CYCGRG 4 cut(s) 74, 86, 200, 748
AoxI GGCC 2 cut(s) 577, 643
ApeKI GCWGC 1 cut(s) 278
ApoI RAATTY 1 cut(s) 458
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspLEI GCGC 1 cut(s) 352
AspS9I GGNCC 2 cut(s) 187, 744
AvaI CYCGRG 4 cut(s) 74, 86, 200, 748
AvaII GGWCC 2 cut(s) 187, 744
BanI GGYRCC 2 cut(s) 307, 349
BbsI GAAGAC 2 cut(s) 201, 735
BbvI GCAGC 1 cut(s) 290
BccI CCATC 3 cut(s) 635, 677, 734
BceAI ACGGC 1 cut(s) 85
BciT130I CCWGG 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 348
BfoI RGCGCY 1 cut(s) 353
BisI GCNGC 4 cut(s) 110, 279, 353, 580
BlsI GCNGC 4 cut(s) 111, 280, 354, 581
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 2 cut(s) 187, 744
BmeT110I CYCGRG 4 cut(s) 74, 86, 200, 748
BmgT120I GGNCC 2 cut(s) 187, 744
BmiI GGNNCC 4 cut(s) 80, 309, 351, 745
BmrFI CCNGG 1 cut(s) 163
BmsI GCATC 3 cut(s) 50, 405, 575
BpiI GAAGAC 2 cut(s) 201, 735
Bpu10I CCTNAGC 1 cut(s) 129
Bsa29I ATCGAT 2 cut(s) 168, 681
BsaHI GRCGYC 1 cut(s) 350
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 405
Bse118I RCCGGY 1 cut(s) 124
BseBI CCWGG 1 cut(s) 163
BseCI ATCGAT 2 cut(s) 168, 681
BseGI GGATG 1 cut(s) 364
BseLI CCNNNNNNNGG 2 cut(s) 317, 405
BseMII CTCAG 5 cut(s) 62, 143, 260, 359, 638
BseXI GCAGC 1 cut(s) 290
Bsh1285I CGRYCG 2 cut(s) 229, 665
BshFI GGCC 2 cut(s) 579, 645
BshNI GGYRCC 2 cut(s) 307, 349
BshVI ATCGAT 2 cut(s) 168, 681
BsiEI CGRYCG 2 cut(s) 229, 665
BsiHKCI CYCGRG 4 cut(s) 74, 86, 200, 748
BsiSI CCGG 2 cut(s) 125, 356
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 317, 405
BsmAI GTCTC 1 cut(s) 348
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 2 cut(s) 579, 645
BsoBI CYCGRG 4 cut(s) 74, 86, 200, 748
Bsp1407I TGTACA 2 cut(s) 21, 381
Bsp143I GATC 9 cut(s) 165, 214, 226, 299, 320, 502, 625, 658, 678
BspACI CCGC 9 cut(s) 5, 98, 110, 263, 334, 353, 405, 450, 580
BspANI GGCC 2 cut(s) 579, 645
BspCNI CTCAG 5 cut(s) 61, 142, 261, 358, 639
BspDI ATCGAT 2 cut(s) 168, 681
BspLI GGNNCC 4 cut(s) 80, 309, 351, 745
BspPI GGATC 4 cut(s) 173, 307, 328, 620
BspT107I GGYRCC 2 cut(s) 307, 349
BsrFI RCCGGY 1 cut(s) 124
BsrGI TGTACA 2 cut(s) 21, 381
BssAI RCCGGY 1 cut(s) 124
BssMI GATC 9 cut(s) 165, 214, 226, 299, 320, 502, 625, 658, 678
BssNAI GTATAC 1 cut(s) 529
BssNI GRCGYC 1 cut(s) 350
Bst1107I GTATAC 1 cut(s) 529
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 2 cut(s) 520, 536
Bst6I CTCTTC 1 cut(s) 248
BstACI GRCGYC 1 cut(s) 350
BstAUI TGTACA 2 cut(s) 21, 381
BstC8I GCNNGC 3 cut(s) 261, 566, 672
BstDEI CTNAG 5 cut(s) 48, 129, 269, 345, 647
BstF5I GGATG 1 cut(s) 364
BstH2I RGCGCY 1 cut(s) 353
BstHHI GCGC 1 cut(s) 352
BstKTI GATC 9 cut(s) 168, 217, 229, 302, 323, 505, 628, 661, 681
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 9 cut(s) 165, 214, 226, 299, 320, 502, 625, 658, 678
BstMCI CGRYCG 2 cut(s) 229, 665
BstMWI GCNNNNNNNGC 6 cut(s) 106, 265, 287, 570, 579, 585
BstNI CCWGG 1 cut(s) 163
BstSCI CCNGG 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 290
BstV2I GAAGAC 2 cut(s) 201, 735
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BstZ17I GTATAC 1 cut(s) 529
Bsu15I ATCGAT 2 cut(s) 168, 681
BsuRI GGCC 2 cut(s) 579, 645
BsuTUI ATCGAT 2 cut(s) 168, 681
BtsCI GGATG 1 cut(s) 364
BtsI GCAGTG 1 cut(s) 425
BtsIMutI CAGTG 3 cut(s) 56, 425, 541
Cac8I GCNNGC 3 cut(s) 261, 566, 672
CaiI CAGNNNCTG 1 cut(s) 629
CfoI GCGC 1 cut(s) 352
Cfr10I RCCGGY 1 cut(s) 124
Cfr13I GGNCC 2 cut(s) 187, 744
ClaI ATCGAT 2 cut(s) 168, 681
Csp6I GTAC 3 cut(s) 22, 382, 632
CviAII CATG 1 cut(s) 725
CviQI GTAC 3 cut(s) 22, 382, 632
DdeI CTNAG 5 cut(s) 48, 129, 269, 345, 647
DinI GGCGCC 1 cut(s) 351
DpnI GATC 9 cut(s) 167, 216, 228, 301, 322, 504, 627, 660, 680
DpnII GATC 9 cut(s) 165, 214, 226, 299, 320, 502, 625, 658, 678
DraIII CACNNNGTG 1 cut(s) 19
EaeI YGGCCR 1 cut(s) 577
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
EciI GGCGGA 2 cut(s) 20, 278
Eco47I GGWCC 2 cut(s) 187, 744
Eco57I CTGAAG 1 cut(s) 615
Eco88I CYCGRG 4 cut(s) 74, 86, 200, 748
EcoO109I RGGNCCY 2 cut(s) 187, 744
EcoRII CCWGG 1 cut(s) 161
EgeI GGCGCC 1 cut(s) 351
EheI GGCGCC 1 cut(s) 351
FaeI CATG 1 cut(s) 728
FaiI YATR 5 cut(s) 62, 425, 529, 704, 726
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FaqI GGGAC 1 cut(s) 68
FatI CATG 1 cut(s) 724
FauI CCCGC 3 cut(s) 327, 398, 457
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 4 cut(s) 110, 279, 353, 580
FokI GGATG 1 cut(s) 371
Fsp4HI GCNGC 4 cut(s) 110, 279, 353, 580
GlaI GCGC 1 cut(s) 351
GluI GCNGC 4 cut(s) 110, 279, 353, 580
HaeII RGCGCY 1 cut(s) 353
HaeIII GGCC 2 cut(s) 579, 645
HapII CCGG 2 cut(s) 125, 356
HhaI GCGC 1 cut(s) 352
Hin1I GRCGYC 1 cut(s) 350
Hin1II CATG 1 cut(s) 728
Hin6I GCGC 1 cut(s) 350
HinP1I GCGC 1 cut(s) 350
HinfI GANTC 2 cut(s) 46, 393
HpaII CCGG 2 cut(s) 125, 356
Hpy166II GTNNAC 3 cut(s) 22, 120, 529
Hpy188I TCNGA 7 cut(s) 181, 299, 325, 492, 507, 516, 625
Hpy188III TCNNGA 7 cut(s) 76, 86, 131, 212, 218, 318, 466
Hpy8I GTNNAC 3 cut(s) 22, 120, 529
Hpy99I CGWCG 5 cut(s) 224, 227, 442, 481, 563
HpyAV CCTTC 5 cut(s) 92, 155, 383, 590, 703
HpyCH4III ACNGT 2 cut(s) 520, 536
HpyCH4IV ACGT 2 cut(s) 558, 732
HpyCH4V TGCA 5 cut(s) 290, 418, 486, 568, 588
HpyF10VI GCNNNNNNNGC 6 cut(s) 106, 265, 287, 570, 579, 585
HpyF3I CTNAG 5 cut(s) 48, 129, 269, 345, 647
HpySE526I ACGT 2 cut(s) 558, 732
Hsp92I GRCGYC 1 cut(s) 350
Hsp92II CATG 1 cut(s) 728
HspAI GCGC 1 cut(s) 350
KasI GGCGCC 1 cut(s) 349
Kzo9I GATC 9 cut(s) 165, 214, 226, 299, 320, 502, 625, 658, 678
LmnI GCTCC 2 cut(s) 38, 265
Lsp1109I GCAGC 1 cut(s) 290
LweI GCATC 3 cut(s) 50, 405, 575
MaeII ACGT 2 cut(s) 558, 732
MaeIII GTNAC 1 cut(s) 715
MalI GATC 9 cut(s) 167, 216, 228, 301, 322, 504, 627, 660, 680
MboI GATC 9 cut(s) 165, 214, 226, 299, 320, 502, 625, 658, 678
MboII GAAGA 6 cut(s) 206, 251, 262, 265, 440, 740
MflI RGATCY 1 cut(s) 625
MluCI AATT 1 cut(s) 458
Mly113I GGCGCC 1 cut(s) 350
MlyI GAGTC 1 cut(s) 40
MmeI TCCRAC 1 cut(s) 539
MseI TTAA 1 cut(s) 636
MspI CCGG 2 cut(s) 125, 356
MspR9I CCNGG 1 cut(s) 163
Mva1269I GAATGC 1 cut(s) 486
MvaI CCWGG 1 cut(s) 163
MwoI GCNNNNNNNGC 6 cut(s) 106, 265, 287, 570, 579, 585
NarI GGCGCC 1 cut(s) 350
NdeII GATC 9 cut(s) 165, 214, 226, 299, 320, 502, 625, 658, 678
NlaIII CATG 1 cut(s) 728
NlaIV GGNNCC 4 cut(s) 80, 309, 351, 745
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 1 cut(s) 715
PaeR7I CTCGAG 2 cut(s) 200, 748
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 308
PctI GAATGC 1 cut(s) 486
PfeI GAWTC 1 cut(s) 393
PfoI TCCNGGA 1 cut(s) 161
PkrI GCNGC 4 cut(s) 111, 280, 354, 581
Ple19I CGATCG 1 cut(s) 229
PleI GAGTC 1 cut(s) 40
PluTI GGCGCC 1 cut(s) 353
PpsI GAGTC 1 cut(s) 40
PpuMI RGGWCCY 2 cut(s) 187, 744
Psp5II RGGWCCY 2 cut(s) 187, 744
Psp6I CCWGG 1 cut(s) 161
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 4 cut(s) 80, 309, 351, 745
PspPI GGNCC 2 cut(s) 187, 744
PspPPI RGGWCCY 2 cut(s) 187, 744
PspXI VCTCGAGB 2 cut(s) 200, 748
PstNI CAGNNNCTG 1 cut(s) 629
PsuI RGATCY 1 cut(s) 625
PvuI CGATCG 1 cut(s) 229
RsaI GTAC 3 cut(s) 23, 383, 633
RsaNI GTAC 3 cut(s) 22, 382, 632
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 4 cut(s) 110, 279, 353, 580
Sau3AI GATC 9 cut(s) 165, 214, 226, 299, 320, 502, 625, 658, 678
Sau96I GGNCC 2 cut(s) 187, 744
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 1 cut(s) 163
SfaNI GCATC 3 cut(s) 50, 405, 575
SfoI GGCGCC 1 cut(s) 351
Sfr274I CTCGAG 2 cut(s) 200, 748
SinI GGWCC 2 cut(s) 187, 744
SlaI CTCGAG 2 cut(s) 200, 748
SmlI CTYRAG 2 cut(s) 200, 748
SmoI CTYRAG 2 cut(s) 200, 748
Sse9I AATT 1 cut(s) 458
SsiI CCGC 9 cut(s) 5, 98, 110, 263, 334, 353, 405, 450, 580
SspDI GGCGCC 1 cut(s) 349
StyD4I CCNGG 1 cut(s) 161
TaaI ACNGT 2 cut(s) 520, 536
TaiI ACGT 2 cut(s) 561, 735
TaqII GACCGA 1 cut(s) 651
TasI AATT 1 cut(s) 458
TatI WGTACW 3 cut(s) 21, 381, 631
TauI GCSGC 3 cut(s) 112, 355, 582
TfiI GAWTC 1 cut(s) 393
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 3 cut(s) 56, 425, 541
TseFI GTSAC 1 cut(s) 715
TseI GCWGC 1 cut(s) 278
Tsp45I GTSAC 1 cut(s) 715
TspDTI ATGAA 1 cut(s) 741
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 3 cut(s) 56, 425, 541
VpaK11BI GGWCC 2 cut(s) 187, 744
XapI RAATTY 1 cut(s) 458
XhoI CTCGAG 2 cut(s) 200, 748
XmiI GTMKAC 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.