Rw2G054110

Phosducin-like protein 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002093
Physical Location & Seq
Forward (+)
81800788 .. 81803162
2375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G054110.1

Sequence Viewer

Length: 579 bp
ATGATGGAACATCACTTTGTGTACAAAGACTTGGACGGAGCGTCGACGCAGTGGGACGATATACAGGCAAAGCTAGAGAATATTCGGAAGAAGCCACCGGCGTTCTATCCGCCAGCTTTCACTCCTTCGGAGGACGAACAGGACCTCGAAGATCTCGAAGACGATCCCGATCTCGACGACGATCGATTCCTCGAGGAATACAGAGAAGCAGCTAAGGTTGCGAGGTTTGGATCGGTGGTTCGTATTTCAGGATCAGATTTTGTGCGTGAGGTTTCGCAGGCTCCGCCTGAAGTTTGGGTGGTTGTGATTCTCTACAAAGAAGGAATTGCAGAGTGCGGGTTGTTGAAGCAGTGTTTGGAAGATCTGGCAACCAAGTACCCCGCAACAAAATTTGTCAAAATAATATCCACAGAGTGTAACCTTCCCAGTCTTATAGTGTACAACAGTGGCGCTGTGAAAGCCAATCACGTTGGGTTGCGCAGCTTTGGTCGGAAATGCACTTCTGAAGTTCGCAAGAGATTCATGGAGAAAGTTGTGATACAACACGAAGATCACGATGATGGCTACTCAAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phosducin

Protein Analysis

192

Amino Acids

21.88

Weight (kDa)

4.83

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phosducin PF02114 57 - 157 1.6e-07 Phosducin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 191
Acc16I TGCGCA 1 cut(s) 479
AccI GTMKAC 1 cut(s) 44
AciI CCGC 4 cut(s) 110, 284, 336, 381
AclWI GGATC 3 cut(s) 158, 238, 259
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 2 cut(s) 309, 525
AdeI CACNNNGTG 2 cut(s) 19, 414
AfaI GTAC 3 cut(s) 23, 377, 440
AgsI TTSAA 1 cut(s) 346
AhdI GACNNNNNGTC 1 cut(s) 40
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AloI GAACNNNNNNTCC 2 cut(s) 222, 254
AluBI AGCT 4 cut(s) 73, 116, 212, 483
AluI AGCT 4 cut(s) 73, 116, 212, 483
AlwI GGATC 3 cut(s) 158, 238, 259
Ama87I CYCGRG 1 cut(s) 191
ApeKI GCWGC 2 cut(s) 209, 480
ApoI RAATTY 1 cut(s) 389
AspLEI GCGC 2 cut(s) 452, 480
AspS9I GGNCC 1 cut(s) 142
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 1 cut(s) 142
BbsI GAAGAC 1 cut(s) 165
BbvI GCAGC 2 cut(s) 221, 492
BccI CCATC 1 cut(s) 554
BfaI CTAG 1 cut(s) 74
BfoI RGCGCY 1 cut(s) 453
BglII AGATCT 2 cut(s) 151, 361
BisI GCNGC 2 cut(s) 210, 481
BlsI GCNGC 2 cut(s) 211, 482
Bme18I GGWCC 1 cut(s) 142
BmeRI GACNNNNNGTC 1 cut(s) 40
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 282
BmrI ACTGGG 1 cut(s) 420
BmuI ACTGGG 1 cut(s) 420
BpiI GAAGAC 1 cut(s) 165
Bpu10I CCTNAGC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 553
Bsa29I ATCGAT 1 cut(s) 184
BsaBI GATNNNNATC 1 cut(s) 168
Bse118I RCCGGY 1 cut(s) 97
Bse1I ACTGG 1 cut(s) 426
Bse8I GATNNNNATC 1 cut(s) 168
BseCI ATCGAT 1 cut(s) 184
BseJI GATNNNNATC 1 cut(s) 168
BseNI ACTGG 1 cut(s) 426
BseXI GCAGC 2 cut(s) 221, 492
Bsh1285I CGRYCG 1 cut(s) 184
BshVI ATCGAT 1 cut(s) 184
BsiEI CGRYCG 1 cut(s) 184
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 1 cut(s) 98
BslFI GGGAC 1 cut(s) 68
BsmFI GGGAC 1 cut(s) 68
BsoBI CYCGRG 1 cut(s) 191
Bsp1407I TGTACA 2 cut(s) 21, 438
Bsp143I GATC 8 cut(s) 151, 163, 169, 181, 230, 251, 361, 550
BspACI CCGC 4 cut(s) 110, 284, 336, 381
BspDI ATCGAT 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 282
BspPI GGATC 3 cut(s) 158, 238, 259
BsrFI RCCGGY 1 cut(s) 97
BsrGI TGTACA 2 cut(s) 21, 438
BsrI ACTGG 1 cut(s) 426
BssAI RCCGGY 1 cut(s) 97
BssMI GATC 8 cut(s) 151, 163, 169, 181, 230, 251, 361, 550
Bst4CI ACNGT 1 cut(s) 446
BstAUI TGTACA 2 cut(s) 21, 438
BstC8I GCNNGC 2 cut(s) 114, 279
BstDEI CTNAG 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 2 cut(s) 452, 480
BstKTI GATC 8 cut(s) 154, 166, 172, 184, 233, 254, 364, 553
BstMBI GATC 8 cut(s) 151, 163, 169, 181, 230, 251, 361, 550
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 3 cut(s) 218, 283, 458
BstV1I GCAGC 2 cut(s) 221, 492
BstV2I GAAGAC 1 cut(s) 165
BstX2I RGATCY 2 cut(s) 151, 361
BstYI RGATCY 2 cut(s) 151, 361
Bsu15I ATCGAT 1 cut(s) 184
BsuTUI ATCGAT 1 cut(s) 184
BtsI GCAGTG 2 cut(s) 56, 356
BtsIMutI CAGTG 3 cut(s) 56, 356, 451
Cac8I GCNNGC 2 cut(s) 114, 279
CfoI GCGC 2 cut(s) 452, 480
Cfr10I RCCGGY 1 cut(s) 97
Cfr13I GGNCC 1 cut(s) 142
ClaI ATCGAT 1 cut(s) 184
CseI GACGC 2 cut(s) 30, 55
Csp6I GTAC 3 cut(s) 22, 376, 439
CviAII CATG 1 cut(s) 523
CviJI RGCY 8 cut(s) 73, 94, 116, 212, 281, 461, 483, 564
CviKI_1 RGCY 8 cut(s) 73, 94, 116, 212, 281, 461, 483, 564
CviQI GTAC 3 cut(s) 22, 376, 439
DdeI CTNAG 1 cut(s) 213
DpnI GATC 8 cut(s) 153, 165, 171, 183, 232, 253, 363, 552
DpnII GATC 8 cut(s) 151, 163, 169, 181, 230, 251, 361, 550
DraIII CACNNNGTG 2 cut(s) 19, 414
DriI GACNNNNNGTC 1 cut(s) 40
Eam1105I GACNNNNNGTC 1 cut(s) 40
EciI GGCGGA 2 cut(s) 99, 273
Eco47I GGWCC 1 cut(s) 142
Eco57I CTGAAG 2 cut(s) 309, 525
Eco88I CYCGRG 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 142
FaeI CATG 1 cut(s) 526
FaiI YATR 3 cut(s) 62, 434, 524
FaqI GGGAC 1 cut(s) 68
FatI CATG 1 cut(s) 522
FauI CCCGC 2 cut(s) 329, 388
FblI GTMKAC 1 cut(s) 44
Fnu4HI GCNGC 2 cut(s) 210, 481
Fsp4HI GCNGC 2 cut(s) 210, 481
FspBI CTAG 1 cut(s) 74
FspI TGCGCA 1 cut(s) 479
GlaI GCGC 2 cut(s) 451, 479
GluI GCNGC 2 cut(s) 210, 481
HaeII RGCGCY 1 cut(s) 453
HapII CCGG 1 cut(s) 98
HgaI GACGC 2 cut(s) 30, 55
HhaI GCGC 2 cut(s) 452, 480
Hin1II CATG 1 cut(s) 526
Hin6I GCGC 2 cut(s) 450, 478
HinP1I GCGC 2 cut(s) 450, 478
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HinfI GANTC 3 cut(s) 186, 307, 519
HpaII CCGG 1 cut(s) 98
Hpy166II GTNNAC 3 cut(s) 22, 45, 439
Hpy188I TCNGA 5 cut(s) 87, 130, 256, 492, 505
Hpy188III TCNNGA 5 cut(s) 155, 167, 173, 249, 554
Hpy8I GTNNAC 3 cut(s) 22, 45, 439
Hpy99I CGWCG 4 cut(s) 46, 49, 179, 182
HpyAV CCTTC 3 cut(s) 135, 314, 431
HpyCH4III ACNGT 1 cut(s) 446
HpyCH4IV ACGT 1 cut(s) 468
HpyCH4V TGCA 2 cut(s) 329, 498
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 283, 458
HpyF3I CTNAG 1 cut(s) 213
HpySE526I ACGT 1 cut(s) 468
Hsp92II CATG 1 cut(s) 526
HspAI GCGC 2 cut(s) 450, 478
Kzo9I GATC 8 cut(s) 151, 163, 169, 181, 230, 251, 361, 550
LmnI GCTCC 2 cut(s) 38, 286
LpnPI CCDG 9 cut(s) 50, 111, 125, 126, 234, 263, 300, 350, 439
Lsp1109I GCAGC 2 cut(s) 221, 492
MaeI CTAG 1 cut(s) 74
MaeII ACGT 1 cut(s) 468
MaeIII GTNAC 1 cut(s) 416
MalI GATC 8 cut(s) 153, 165, 171, 183, 232, 253, 363, 552
MboI GATC 8 cut(s) 151, 163, 169, 181, 230, 251, 361, 550
MboII GAAGA 5 cut(s) 100, 161, 170, 371, 560
MflI RGATCY 2 cut(s) 151, 361
MluCI AATT 2 cut(s) 324, 389
MmeI TCCRAC 1 cut(s) 470
MnlI CCTC 6 cut(s) 124, 155, 187, 200, 216, 262
MslI CAYNNNNRTG 1 cut(s) 558
MspI CCGG 1 cut(s) 98
MwoI GCNNNNNNNGC 3 cut(s) 218, 283, 458
NdeII GATC 8 cut(s) 151, 163, 169, 181, 230, 251, 361, 550
NlaIII CATG 1 cut(s) 526
NlaIV GGNNCC 1 cut(s) 282
NsbI TGCGCA 1 cut(s) 479
PaeR7I CTCGAG 1 cut(s) 191
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 552
PfeI GAWTC 3 cut(s) 186, 307, 519
PkrI GCNGC 2 cut(s) 211, 482
Ple19I CGATCG 1 cut(s) 184
PpuMI RGGWCCY 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 282
PspPI GGNCC 1 cut(s) 142
PspPPI RGGWCCY 1 cut(s) 142
PspXI VCTCGAGB 1 cut(s) 191
PsuI RGATCY 2 cut(s) 151, 361
PvuI CGATCG 1 cut(s) 184
RsaI GTAC 3 cut(s) 23, 377, 440
RsaNI GTAC 3 cut(s) 22, 376, 439
RseI CAYNNNNRTG 1 cut(s) 558
SalI GTCGAC 1 cut(s) 43
SatI GCNGC 2 cut(s) 210, 481
Sau3AI GATC 8 cut(s) 151, 163, 169, 181, 230, 251, 361, 550
Sau96I GGNCC 1 cut(s) 142
Sfr274I CTCGAG 1 cut(s) 191
SgrAI CRCCGGYG 1 cut(s) 97
SgrDI CGTCGACG 1 cut(s) 43
SinI GGWCC 1 cut(s) 142
SlaI CTCGAG 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 2 cut(s) 191, 568
SmoI CTYRAG 2 cut(s) 191, 568
Sse9I AATT 2 cut(s) 324, 389
SsiI CCGC 4 cut(s) 110, 284, 336, 381
SspI AATATT 1 cut(s) 82
SspMI CTAG 1 cut(s) 74
TaaI ACNGT 1 cut(s) 446
TaiI ACGT 1 cut(s) 471
TaqI TCGA 6 cut(s) 44, 147, 156, 174, 184, 192
TasI AATT 2 cut(s) 324, 389
TatI WGTACW 2 cut(s) 21, 438
TfiI GAWTC 3 cut(s) 186, 307, 519
TscAI CASTG 3 cut(s) 56, 356, 451
TseI GCWGC 2 cut(s) 209, 480
TspDTI ATGAA 1 cut(s) 511
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 3 cut(s) 56, 356, 451
VpaK11BI GGWCC 1 cut(s) 142
XapI RAATTY 1 cut(s) 389
XhoI CTCGAG 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 44
XspI CTAG 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.