MD09G1156300.v1.1

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003217
Physical Location & Seq
Forward (+)
12454745 .. 12457854
3110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1156300.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGCTGTGGCTTTGAAGCTCCTGAAGCTACTCCGACCACTGCAGACCACCTCCACAATCCTCCACCGCAATCTCAGCCCACAGCTCTCGAGCATTCGCAGTGTAACCAGAGTCTTCTCAGACTCTCCTCTTCTAACCGAGCCCCTCCCGGCTTCACCGCCGCTAAACCCTCATTTTCCTTCCCCGTCGTCCCTGAACCAGTACGAGCTTGCCAAGTTCGCCTCCATTGCCGAAACCTGGTGGGATTCTGAAGGGCCGTTCAAACCATTGCATCTGCTGAATTCTACAAGGCTTGCTTTCATTCGCTCCACGCTTTGCCGACATTTCGGGAGGGATCCGTTATCTCCTAGGCCATTCGATGGCCTCAAATTTATCGATGTTGGCTGTGGTGGTGGAATTGTTTCTGAGCCTTTAGCTCGAATGGGAGCTACTGTGACAGGAGTTGATGCGGTTGAGAAAAATATCAAGATAGCACGTGTTCACGCTGATTTGGATCCAGTGACCTCGAGAATTGAATACCAATACACTACAGCTGAAAAGCTAGTTGAGGAACAGAAGAATTTTGATGCTGTTTTTGCTTTAGAGGTAATTGAGCATGTAGCCGACCCTACCGAATTCTGCAAATCTCTATCAGCATTAACTGTCCCCGGTGGAGCAACTGTTATTTCAACCATTAACCGTTCTATGAGAGCGTATGCTACTGCCATTTTTGCAGCAGAGTATATCCTCCAATGGCTTCCTAAAGGTACACATCAATGGTCCAGTTTTCTCGCTCCCGAAGAACTAGCCCTGATCCTGGAGCGTGCTTCTATTTCAGTCCAAGAGATGGCTGGGTGCGAGTACGACCCTTTGTCAGGTAGATGGTCTTTATCCGACGATGTTAGTGTAAATTTTATTGCTTATGGTGCTAAAACAATAACATCCAATTTATAA

Protein Analysis

311

Amino Acids

34.07

Weight (kDa)

6.0

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 112 - 269 2.6e-20 Methyltransferase domain
Methyltransf_31 PF13847 120 - 256 1.1e-14 Methyltransferase domain
CMAS PF02353 121 - 234 6e-07 Mycolic acid cyclopropane synthetase
PrmA PF06325 123 - 225 1.1e-06 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_11 PF08241 125 - 222 1.8e-18 Methyltransferase domain
Methyltransf_25 PF13649 125 - 218 6.5e-15 Methyltransferase domain
Methyltransf_12 PF08242 125 - 218 5.3e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 931
AccB7I CCANNNNNTGG 2 cut(s) 359, 826
AciI CCGC 4 cut(s) 67, 158, 161, 449
AclWI GGATC 5 cut(s) 329, 342, 488, 501, 787
AcsI RAATTY 5 cut(s) 280, 368, 559, 614, 889
AcuI CTGAAG 2 cut(s) 44, 270
AcvI CACGTG 1 cut(s) 476
AfaI GTAC 3 cut(s) 203, 748, 842
AfiI CCNNNNNNNGG 5 cut(s) 237, 359, 796, 826, 854
AflIII ACRYGT 1 cut(s) 475
AgsI TTSAA 4 cut(s) 16, 262, 515, 669
AhdI GACNNNNNGTC 1 cut(s) 850
AjnI CCWGG 2 cut(s) 236, 795
AluBI AGCT 8 cut(s) 19, 28, 85, 208, 416, 428, 533, 541
AluI AGCT 8 cut(s) 19, 28, 85, 208, 416, 428, 533, 541
AlwI GGATC 5 cut(s) 329, 342, 488, 501, 787
Ama87I CYCGRG 2 cut(s) 88, 505
AoxI GGCC 3 cut(s) 254, 350, 361
ApeKI GCWGC 1 cut(s) 713
ApoI RAATTY 5 cut(s) 280, 368, 559, 614, 889
Asp700I GAANNNNTTC 1 cut(s) 400
AspA2I CCTAGG 1 cut(s) 347
AspS9I GGNCC 2 cut(s) 254, 759
AsuC2I CCSGG 2 cut(s) 149, 648
AsuHPI GGTGA 1 cut(s) 147
AvaI CYCGRG 2 cut(s) 88, 505
AvaII GGWCC 1 cut(s) 759
AvrII CCTAGG 1 cut(s) 347
BamHI GGATCC 2 cut(s) 334, 493
BanII GRGCYC 1 cut(s) 144
BbrPI CACGTG 1 cut(s) 476
BbsI GAAGAC 1 cut(s) 106
BbvI GCAGC 1 cut(s) 725
BccI CCATC 3 cut(s) 353, 820, 855
BceAI ACGGC 1 cut(s) 241
BciT130I CCWGG 2 cut(s) 238, 797
BcnI CCSGG 2 cut(s) 149, 648
BfaI CTAG 3 cut(s) 348, 542, 785
BfmI CTRYAG 2 cut(s) 41, 528
BisI GCNGC 2 cut(s) 161, 714
BlnI CCTAGG 1 cut(s) 347
BlsI GCNGC 2 cut(s) 162, 715
Bme1390I CCNGG 4 cut(s) 149, 238, 648, 797
Bme18I GGWCC 1 cut(s) 759
BmeRI GACNNNNNGTC 1 cut(s) 850
BmeT110I CYCGRG 2 cut(s) 88, 505
BmgT120I GGNCC 2 cut(s) 254, 759
BmiI GGNNCC 2 cut(s) 336, 495
BmrFI CCNGG 4 cut(s) 149, 238, 648, 797
BmsI GCATC 3 cut(s) 280, 436, 556
BpiI GAAGAC 1 cut(s) 106
BpmI CTGGAG 1 cut(s) 818
BpuMI CCSGG 2 cut(s) 149, 648
Bsa29I ATCGAT 1 cut(s) 375
BsaAI YACGTR 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 492
BsaJI CCNNGG 2 cut(s) 347, 646
BsaXI ACNNNNNCTCC 4 cut(s) 417, 447, 645, 675
Bsc4I CCNNNNNNNGG 5 cut(s) 237, 359, 796, 826, 854
Bse1I ACTGG 3 cut(s) 199, 497, 762
Bse3DI GCAATG 2 cut(s) 225, 266
Bse8I GATNNNNATC 1 cut(s) 492
BseBI CCWGG 2 cut(s) 238, 797
BseCI ATCGAT 1 cut(s) 375
BseDI CCNNGG 2 cut(s) 347, 646
BseGI GGATG 1 cut(s) 920
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 5 cut(s) 237, 359, 796, 826, 854
BseMI GCAATG 2 cut(s) 225, 266
BseMII CTCAG 3 cut(s) 88, 132, 396
BseNI ACTGG 3 cut(s) 199, 497, 762
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 1 cut(s) 725
BseYI CCCAGC 1 cut(s) 830
BshFI GGCC 3 cut(s) 256, 352, 363
BshVI ATCGAT 1 cut(s) 375
BsiHKCI CYCGRG 2 cut(s) 88, 505
BsiSI CCGG 2 cut(s) 149, 648
BslFI GGGAC 2 cut(s) 175, 629
BslI CCNNNNNNNGG 5 cut(s) 237, 359, 796, 826, 854
BsmFI GGGAC 2 cut(s) 175, 629
BsmI GAATGC 1 cut(s) 93
BsnI GGCC 3 cut(s) 256, 352, 363
BsoBI CYCGRG 2 cut(s) 88, 505
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 3 cut(s) 334, 493, 792
BspACI CCGC 4 cut(s) 67, 158, 161, 449
BspANI GGCC 3 cut(s) 256, 352, 363
BspCNI CTCAG 3 cut(s) 87, 131, 397
BspDI ATCGAT 1 cut(s) 375
BspLI GGNNCC 2 cut(s) 336, 495
BspMAI CTGCAG 1 cut(s) 45
BspPI GGATC 5 cut(s) 329, 342, 488, 501, 787
BsrDI GCAATG 2 cut(s) 225, 266
BsrI ACTGG 3 cut(s) 199, 497, 762
BssECI CCNNGG 2 cut(s) 347, 646
BssMI GATC 3 cut(s) 334, 493, 792
BssT1I CCWWGG 1 cut(s) 347
Bst2UI CCWGG 2 cut(s) 238, 797
Bst4CI ACNGT 4 cut(s) 433, 643, 661, 680
Bst6I CTCTTC 1 cut(s) 135
BstBAI YACGTR 1 cut(s) 476
BstC8I GCNNGC 3 cut(s) 210, 294, 804
BstDEI CTNAG 3 cut(s) 74, 118, 405
BstENI CCTNNNNNAGG 1 cut(s) 852
BstF5I GGATG 1 cut(s) 920
BstKTI GATC 3 cut(s) 337, 496, 795
BstMBI GATC 3 cut(s) 334, 493, 792
BstMWI GCNNNNNNNGC 7 cut(s) 25, 75, 218, 227, 575, 710, 905
BstNI CCWGG 2 cut(s) 238, 797
BstNSI RCATGY 1 cut(s) 599
BstSCI CCNGG 4 cut(s) 147, 236, 646, 795
BstSFI CTRYAG 2 cut(s) 41, 528
BstV1I GCAGC 1 cut(s) 725
BstV2I GAAGAC 1 cut(s) 106
BstX2I RGATCY 2 cut(s) 334, 493
BstYI RGATCY 2 cut(s) 334, 493
Bsu15I ATCGAT 1 cut(s) 375
BsuRI GGCC 3 cut(s) 256, 352, 363
BsuTUI ATCGAT 1 cut(s) 375
BtsCI GGATG 1 cut(s) 920
BtsI GCAGTG 2 cut(s) 38, 106
BtsIMutI CAGTG 3 cut(s) 38, 106, 504
Cac8I GCNNGC 3 cut(s) 210, 294, 804
Cfr13I GGNCC 2 cut(s) 254, 759
ClaI ATCGAT 1 cut(s) 375
CsiI ACCWGGT 1 cut(s) 236
Csp6I GTAC 3 cut(s) 202, 747, 841
CviAII CATG 1 cut(s) 596
CviQI GTAC 3 cut(s) 202, 747, 841
DdeI CTNAG 3 cut(s) 74, 118, 405
DpnI GATC 3 cut(s) 336, 495, 794
DpnII GATC 3 cut(s) 334, 493, 792
DriI GACNNNNNGTC 1 cut(s) 850
Eam1104I CTCTTC 1 cut(s) 135
Eam1105I GACNNNNNGTC 1 cut(s) 850
EarI CTCTTC 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 759
Eco57I CTGAAG 2 cut(s) 44, 270
Eco72I CACGTG 1 cut(s) 476
Eco88I CYCGRG 2 cut(s) 88, 505
EcoNI CCTNNNNNAGG 1 cut(s) 852
EcoRI GAATTC 2 cut(s) 280, 614
EcoRII CCWGG 2 cut(s) 236, 795
EcoT14I CCWWGG 1 cut(s) 347
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 347
FaeI CATG 1 cut(s) 599
FaiI YATR 6 cut(s) 597, 686, 696, 723, 903, 931
FalI AAGNNNNNCTT 2 cut(s) 280, 312
FaqI GGGAC 2 cut(s) 175, 629
FatI CATG 1 cut(s) 595
Fnu4HI GCNGC 2 cut(s) 161, 714
FokI GGATG 1 cut(s) 907
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 2 cut(s) 161, 714
FspBI CTAG 3 cut(s) 348, 542, 785
GluI GCNGC 2 cut(s) 161, 714
GsaI CCCAGC 1 cut(s) 834
GsuI CTGGAG 1 cut(s) 818
HaeIII GGCC 3 cut(s) 256, 352, 363
HapII CCGG 2 cut(s) 149, 648
Hin1II CATG 1 cut(s) 599
HinfI GANTC 3 cut(s) 111, 122, 245
HpaII CCGG 2 cut(s) 149, 648
HphI GGTGA 1 cut(s) 147
Hpy166II GTNNAC 2 cut(s) 481, 749
Hpy188I TCNGA 5 cut(s) 35, 121, 250, 406, 874
Hpy188III TCNNGA 6 cut(s) 22, 88, 328, 466, 507, 776
Hpy8I GTNNAC 2 cut(s) 481, 749
Hpy99I CGWCG 2 cut(s) 190, 878
HpyAV CCTTC 2 cut(s) 189, 245
HpyCH4III ACNGT 4 cut(s) 433, 643, 661, 680
HpyCH4IV ACGT 1 cut(s) 475
HpyCH4V TGCA 4 cut(s) 43, 271, 621, 713
HpyF10VI GCNNNNNNNGC 7 cut(s) 25, 75, 218, 227, 575, 710, 905
HpyF3I CTNAG 3 cut(s) 74, 118, 405
HpySE526I ACGT 1 cut(s) 475
Hsp92II CATG 1 cut(s) 599
Kzo9I GATC 3 cut(s) 334, 493, 792
LmnI GCTCC 6 cut(s) 24, 311, 425, 653, 778, 799
Lsp1109I GCAGC 1 cut(s) 725
LweI GCATC 3 cut(s) 280, 436, 556
MabI ACCWGGT 1 cut(s) 236
MaeI CTAG 3 cut(s) 348, 542, 785
MaeII ACGT 1 cut(s) 475
MaeIII GTNAC 3 cut(s) 103, 433, 499
MalI GATC 3 cut(s) 336, 495, 794
MboI GATC 3 cut(s) 334, 493, 792
MboII GAAGA 4 cut(s) 106, 122, 568, 791
MflI RGATCY 2 cut(s) 334, 493
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 9 cut(s) 280, 368, 396, 510, 559, 588, 614, 889, 925
MlyI GAGTC 2 cut(s) 116, 120
MmeI TCCRAC 2 cut(s) 58, 897
MroXI GAANNNNTTC 1 cut(s) 400
MseI TTAA 2 cut(s) 638, 675
MslI CAYNNNNRTG 1 cut(s) 754
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 2 cut(s) 149, 648
MspR9I CCNGG 4 cut(s) 149, 238, 648, 797
Mva1269I GAATGC 1 cut(s) 93
MvaI CCWGG 2 cut(s) 238, 797
MwoI GCNNNNNNNGC 7 cut(s) 25, 75, 218, 227, 575, 710, 905
NciI CCSGG 2 cut(s) 149, 648
NdeII GATC 3 cut(s) 334, 493, 792
NlaIII CATG 1 cut(s) 599
NlaIV GGNNCC 2 cut(s) 336, 495
NmuCI GTSAC 2 cut(s) 433, 499
NspI RCATGY 1 cut(s) 599
PaeR7I CTCGAG 2 cut(s) 88, 505
PctI GAATGC 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 400
PfeI GAWTC 1 cut(s) 245
PflMI CCANNNNNTGG 2 cut(s) 359, 826
PfoI TCCNGGA 1 cut(s) 795
PkrI GCNGC 2 cut(s) 162, 715
PleI GAGTC 2 cut(s) 116, 119
PmaCI CACGTG 1 cut(s) 476
PmlI CACGTG 1 cut(s) 476
PpsI GAGTC 2 cut(s) 116, 119
Ppu21I YACGTR 1 cut(s) 476
PsiI TTATAA 1 cut(s) 931
Psp6I CCWGG 2 cut(s) 236, 795
PspCI CACGTG 1 cut(s) 476
PspFI CCCAGC 1 cut(s) 830
PspGI CCWGG 2 cut(s) 236, 795
PspN4I GGNNCC 2 cut(s) 336, 495
PspPI GGNCC 2 cut(s) 254, 759
PstI CTGCAG 1 cut(s) 45
PsuI RGATCY 2 cut(s) 334, 493
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 3 cut(s) 203, 748, 842
RsaNI GTAC 3 cut(s) 202, 747, 841
RseI CAYNNNNRTG 1 cut(s) 754
SaqAI TTAA 2 cut(s) 638, 675
SatI GCNGC 2 cut(s) 161, 714
Sau3AI GATC 3 cut(s) 334, 493, 792
Sau96I GGNCC 2 cut(s) 254, 759
SchI GAGTC 2 cut(s) 116, 120
ScrFI CCNGG 4 cut(s) 149, 238, 648, 797
SduI GDGCHC 1 cut(s) 144
SexAI ACCWGGT 1 cut(s) 236
SfaNI GCATC 3 cut(s) 280, 436, 556
SfcI CTRYAG 2 cut(s) 41, 528
Sfr274I CTCGAG 2 cut(s) 88, 505
SinI GGWCC 1 cut(s) 759
SlaI CTCGAG 2 cut(s) 88, 505
SmiMI CAYNNNNRTG 1 cut(s) 754
SmlI CTYRAG 2 cut(s) 88, 505
SmoI CTYRAG 2 cut(s) 88, 505
Sse9I AATT 9 cut(s) 280, 368, 396, 510, 559, 588, 614, 889, 925
SsiI CCGC 4 cut(s) 67, 158, 161, 449
SspMI CTAG 3 cut(s) 348, 542, 785
StyD4I CCNGG 4 cut(s) 147, 236, 646, 795
StyI CCWWGG 1 cut(s) 347
TaaI ACNGT 4 cut(s) 433, 643, 661, 680
TaiI ACGT 1 cut(s) 478
TaqI TCGA 5 cut(s) 89, 357, 375, 418, 506
TasI AATT 9 cut(s) 280, 368, 396, 510, 559, 588, 614, 889, 925
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 2 cut(s) 638, 675
Tru9I TTAA 2 cut(s) 638, 675
TscAI CASTG 3 cut(s) 45, 106, 504
TseFI GTSAC 2 cut(s) 433, 499
TseI GCWGC 1 cut(s) 713
Tsp45I GTSAC 2 cut(s) 433, 499
TspDTI ATGAA 1 cut(s) 289
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 3 cut(s) 45, 106, 504
Van91I CCANNNNNTGG 2 cut(s) 359, 826
VpaK11BI GGWCC 1 cut(s) 759
XagI CCTNNNNNAGG 1 cut(s) 852
XapI RAATTY 5 cut(s) 280, 368, 559, 614, 889
XceI RCATGY 1 cut(s) 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 827
XhoI CTCGAG 2 cut(s) 88, 505
XmaJI CCTAGG 1 cut(s) 347
XmnI GAANNNNTTC 1 cut(s) 400
XspI CTAG 3 cut(s) 348, 542, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.