Rh7CG339700

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0003217
Physical Location & Seq
Forward (+)
38023285 .. 38027317
4033 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG339700.1

Sequence Viewer

Length: 1137 bp
ATGGCCATGACTTGGAAGCTCCTGAAGCAACTCCGAGCACCGTACACCGTCACTGCCTCTATGAGCCTCCACCGAATTCTACCCCCTCAATTGCTGAACTTGCATAATGCCACTAGGCTTTTCTCAAATGGGCCTCCGCTGCCTCCTCCTTCTGGAGAGCCTCCTCTGATTCAACTGCTGCAAAACCCTCCCTCTCCGCCTCCGTCGTCGCTGAACCAGGCAGAGCTTGCTAAGTTTGCCACCATTTCCGGAACCTGGTGGGATTTGGAAGGACCATTCAAACCGTTGCATGCAATGAATCCTACAAGGCTTGCCTTCATTCGCTCAACGCTGTGCCGTCATTTCAGGAGGGACCCGTTATCTGCTAGGCCCTTTGAAGGGCTCAGATTTATTGATGTTGGCTGTGGAGGTGGGATTCTTTCTGAGCCTTTAGCTCGGATGGGAGCTATTGTAACAGGAGTTGATGCTGTTGAGAAAAACATCAAGATAGCTCGCCTTCACGCAGACTTGGATCCAGTGACCTCAAAAATTGAATATCAATGCACTACAGCTGAAAAGCTAGTCGTGGAGCAAAAGAGTTTTGATGCTGTGTTTGCTTTAGAGGTGATTGAGCACGTGGAAAATCCTGCTGATTTCTGTAAATCTCTGTCAGCCTTAACTGTTCCCAGTGGAGCTACTGTGATTTCAACCATTAACCGTTCTATGAGAGCATATGCCACTGCCATAATTGCAGCCGAGTATCTGCTCCGCTGGCTTCCTAAAGGTACACATCAATGGTCAACTTTTCTTACTCCTGAAGAACTAGTCCTGATCCTGGAGTGTGCTTCTATTTCAGTCCAAGAGATGGCTGAGTTTGCATACAACCCTTTGACAGGAAGATGGTCAATATCTGATGATGTTAGTGTAAATTTCATCGCTTATGAGCATGAGATGAATTGCTCAGCAGCTGCTTGGCATCTGGTGTCAAGTGGCGTGGATGTGTACACTGCCCTTGCTGGAGCTGTTGGAGCTCTATATGGTCCACTTCATGGCGGACCAAATGAGCCTGTGTTTAAGGTGCCAACTGAAATCGAATCTGTTGAGAACATTCCAGAGTTCATTAAGGGTGTGAAGAGCAGGTATTGTGCTTACATCTAA

Protein Analysis

378

Amino Acids

41.47

Weight (kDa)

6.08

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 116 - 273 8e-18 Methyltransferase domain
Methyltransf_31 PF13847 126 - 263 2.8e-13 Methyltransferase domain
PrmA PF06325 129 - 231 4e-06 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_11 PF08241 131 - 227 1.2e-19 Methyltransferase domain
Methyltransf_25 PF13649 131 - 224 1.7e-14 Methyltransferase domain
Methyltransf_12 PF08242 131 - 224 3.5e-10 Methyltransferase domain
Citrate_synt PF00285 305 - 371 8.8e-18 Citrate synthase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1107
AccB1I GGYRCC 1 cut(s) 1057
AccB7I CCANNNNNTGG 3 cut(s) 12, 844, 1028
AccIII TCCGGA 1 cut(s) 248
AciI CCGC 4 cut(s) 137, 197, 748, 1032
AclWI GGATC 3 cut(s) 506, 519, 805
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 75, 907
AcuI CTGAAG 2 cut(s) 44, 816
AcvI CACGTG 1 cut(s) 616
AfaI GTAC 3 cut(s) 44, 766, 983
AfiI CCNNNNNNNGG 9 cut(s) 12, 152, 255, 377, 378, 814, 844, 872, 1028
AgsI TTSAA 5 cut(s) 173, 280, 377, 533, 687
AhlI ACTAGT 1 cut(s) 802
AjnI CCWGG 3 cut(s) 216, 254, 813
Alw21I GWGCWC 3 cut(s) 40, 615, 1012
AlwI GGATC 3 cut(s) 506, 519, 805
AlwNI CAGNNNCTG 1 cut(s) 947
Aor13HI TCCGGA 1 cut(s) 248
AoxI GGCC 3 cut(s) 3, 131, 368
ApeKI GCWGC 5 cut(s) 139, 178, 731, 944, 947
ApoI RAATTY 2 cut(s) 75, 907
AspS9I GGNCC 6 cut(s) 131, 272, 352, 369, 1019, 1034
AsuHPI GGTGA 1 cut(s) 616
AvaII GGWCC 4 cut(s) 272, 352, 1019, 1034
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 511
BanI GGYRCC 1 cut(s) 1057
BanII GRGCYC 2 cut(s) 384, 1012
BbrPI CACGTG 1 cut(s) 616
Bbv12I GWGCWC 3 cut(s) 40, 615, 1012
BbvI GCAGC 5 cut(s) 126, 165, 743, 934, 956
BccI CCATC 3 cut(s) 433, 838, 873
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 3 cut(s) 218, 256, 815
BcuI ACTAGT 1 cut(s) 802
BfaI CTAG 4 cut(s) 114, 366, 560, 803
BfmI CTRYAG 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 1107
BisI GCNGC 5 cut(s) 140, 179, 732, 945, 948
BlpI GCTNAGC 1 cut(s) 940
BlsI GCNGC 5 cut(s) 141, 180, 733, 946, 949
Bme1390I CCNGG 3 cut(s) 218, 256, 815
Bme18I GGWCC 4 cut(s) 272, 352, 1019, 1034
BmgT120I GGNCC 6 cut(s) 131, 272, 352, 369, 1019, 1034
BmiI GGNNCC 5 cut(s) 253, 353, 354, 513, 1059
BmrFI CCNGG 3 cut(s) 218, 256, 815
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 3 cut(s) 454, 574, 964
BmuI ACTGGG 1 cut(s) 660
BpmI CTGGAG 3 cut(s) 174, 836, 1017
Bpu1102I GCTNAGC 1 cut(s) 940
BsaAI YACGTR 1 cut(s) 616
BsaWI WCCGGW 1 cut(s) 248
BsaXI ACNNNNNCTCC 4 cut(s) 435, 465, 663, 693
Bsc4I CCNNNNNNNGG 9 cut(s) 12, 152, 255, 377, 378, 814, 844, 872, 1028
Bse1I ACTGG 2 cut(s) 515, 666
Bse3DI GCAATG 1 cut(s) 300
BseAI TCCGGA 1 cut(s) 248
BseBI CCWGG 3 cut(s) 218, 256, 815
BseGI GGATG 2 cut(s) 444, 982
BseLI CCNNNNNNNGG 9 cut(s) 12, 152, 255, 377, 378, 814, 844, 872, 1028
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 4 cut(s) 397, 414, 840, 954
BseNI ACTGG 2 cut(s) 515, 666
BseRI GAGGAG 2 cut(s) 135, 153
BseXI GCAGC 5 cut(s) 126, 165, 743, 934, 956
BshFI GGCC 3 cut(s) 5, 133, 370
BshNI GGYRCC 1 cut(s) 1057
BsiHKAI GWGCWC 3 cut(s) 40, 615, 1012
BsiSI CCGG 1 cut(s) 249
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 9 cut(s) 12, 152, 255, 377, 378, 814, 844, 872, 1028
BsmFI GGGAC 1 cut(s) 365
BsnI GGCC 3 cut(s) 5, 133, 370
Bsp1286I GDGCHC 4 cut(s) 40, 384, 615, 1012
Bsp13I TCCGGA 1 cut(s) 248
Bsp1407I TGTACA 1 cut(s) 981
Bsp143I GATC 2 cut(s) 511, 810
Bsp1720I GCTNAGC 1 cut(s) 940
BspACI CCGC 4 cut(s) 137, 197, 748, 1032
BspANI GGCC 3 cut(s) 5, 133, 370
BspCNI CTCAG 4 cut(s) 396, 415, 841, 953
BspEI TCCGGA 1 cut(s) 248
BspLI GGNNCC 5 cut(s) 253, 353, 354, 513, 1059
BspMI ACCTGC 1 cut(s) 1107
BspPI GGATC 3 cut(s) 506, 519, 805
BspQI GCTCTTC 1 cut(s) 1106
BspT107I GGYRCC 1 cut(s) 1057
BsrDI GCAATG 1 cut(s) 300
BsrGI TGTACA 1 cut(s) 981
BsrI ACTGG 2 cut(s) 515, 666
BssMI GATC 2 cut(s) 511, 810
Bst2UI CCWGG 3 cut(s) 218, 256, 815
Bst4CI ACNGT 6 cut(s) 42, 49, 285, 661, 679, 698
Bst6I CTCTTC 1 cut(s) 1106
BstAPI GCANNNNNTGC 1 cut(s) 227
BstAUI TGTACA 1 cut(s) 981
BstBAI YACGTR 1 cut(s) 616
BstC8I GCNNGC 5 cut(s) 228, 291, 312, 493, 752
BstDEI CTNAG 5 cut(s) 231, 383, 423, 849, 940
BstENI CCTNNNNNAGG 1 cut(s) 870
BstF5I GGATG 2 cut(s) 444, 982
BstKTI GATC 2 cut(s) 514, 813
BstMBI GATC 2 cut(s) 511, 810
BstNI CCWGG 3 cut(s) 218, 256, 815
BstNSI RCATGY 1 cut(s) 293
BstSCI CCNGG 3 cut(s) 216, 254, 813
BstSFI CTRYAG 1 cut(s) 546
BstV1I GCAGC 5 cut(s) 126, 165, 743, 934, 956
BstX2I RGATCY 1 cut(s) 511
BstYI RGATCY 1 cut(s) 511
BsuRI GGCC 3 cut(s) 5, 133, 370
BtgZI GCGATG 1 cut(s) 898
BtsCI GGATG 2 cut(s) 444, 982
BtsI GCAGTG 3 cut(s) 51, 717, 984
BtsIMutI CAGTG 5 cut(s) 51, 522, 673, 717, 984
BveI ACCTGC 1 cut(s) 1107
Cac8I GCNNGC 5 cut(s) 228, 291, 312, 493, 752
CaiI CAGNNNCTG 1 cut(s) 947
Cfr13I GGNCC 6 cut(s) 131, 272, 352, 369, 1019, 1034
CsiI ACCWGGT 1 cut(s) 254
Csp6I GTAC 3 cut(s) 43, 765, 982
CspCI CAANNNNNGTGG 2 cut(s) 954, 989
CviAII CATG 4 cut(s) 7, 290, 926, 1028
CviQI GTAC 3 cut(s) 43, 765, 982
DdeI CTNAG 5 cut(s) 231, 383, 423, 849, 940
DpnI GATC 2 cut(s) 513, 812
DpnII GATC 2 cut(s) 511, 810
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 1106
EarI CTCTTC 1 cut(s) 1106
EciI GGCGGA 2 cut(s) 186, 1047
Ecl136II GAGCTC 1 cut(s) 1010
Eco24I GRGCYC 2 cut(s) 384, 1012
Eco47I GGWCC 4 cut(s) 272, 352, 1019, 1034
Eco53kI GAGCTC 1 cut(s) 1010
Eco57I CTGAAG 2 cut(s) 44, 816
Eco72I CACGTG 1 cut(s) 616
EcoICRI GAGCTC 1 cut(s) 1010
EcoNI CCTNNNNNAGG 1 cut(s) 870
EcoO109I RGGNCCY 2 cut(s) 352, 369
EcoRI GAATTC 1 cut(s) 75
EcoRII CCWGG 3 cut(s) 216, 254, 813
EcoT38I GRGCYC 2 cut(s) 384, 1012
FaeI CATG 4 cut(s) 10, 293, 929, 1031
FaqI GGGAC 1 cut(s) 365
FatI CATG 4 cut(s) 6, 289, 925, 1027
FauNDI CATATG 1 cut(s) 712
Fnu4HI GCNGC 5 cut(s) 140, 179, 732, 945, 948
FokI GGATG 2 cut(s) 451, 989
FriOI GRGCYC 2 cut(s) 384, 1012
Fsp4HI GCNGC 5 cut(s) 140, 179, 732, 945, 948
FspBI CTAG 4 cut(s) 114, 366, 560, 803
GluI GCNGC 5 cut(s) 140, 179, 732, 945, 948
GsuI CTGGAG 3 cut(s) 174, 836, 1017
HaeIII GGCC 3 cut(s) 5, 133, 370
HapII CCGG 1 cut(s) 249
Hin1II CATG 4 cut(s) 10, 293, 929, 1031
HincII GTYRAC 1 cut(s) 780
HindII GTYRAC 1 cut(s) 780
HinfI GANTC 4 cut(s) 169, 298, 415, 1073
HpaII CCGG 1 cut(s) 249
HphI GGTGA 1 cut(s) 616
Hpy166II GTNNAC 6 cut(s) 45, 767, 780, 982, 984, 1022
Hpy188I TCNGA 6 cut(s) 35, 168, 386, 424, 438, 892
Hpy188III TCNNGA 8 cut(s) 22, 153, 249, 346, 484, 794, 808, 1091
Hpy8I GTNNAC 6 cut(s) 45, 767, 780, 982, 984, 1022
Hpy99I CGWCG 2 cut(s) 208, 211
HpyAV CCTTC 5 cut(s) 159, 263, 325, 371, 506
HpyCH4III ACNGT 6 cut(s) 42, 49, 285, 661, 679, 698
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 7 cut(s) 103, 181, 289, 293, 543, 731, 857
HpyF3I CTNAG 5 cut(s) 231, 383, 423, 849, 940
HpySE526I ACGT 1 cut(s) 615
Hsp92II CATG 4 cut(s) 10, 293, 929, 1031
KflI GGGWCCC 1 cut(s) 352
Kpn2I TCCGGA 1 cut(s) 248
Kzo9I GATC 2 cut(s) 511, 810
LguI GCTCTTC 1 cut(s) 1106
LmnI GCTCC 7 cut(s) 24, 443, 568, 671, 750, 998, 1007
Lsp1109I GCAGC 5 cut(s) 126, 165, 743, 934, 956
LweI GCATC 3 cut(s) 454, 574, 964
MabI ACCWGGT 1 cut(s) 254
MaeI CTAG 4 cut(s) 114, 366, 560, 803
MaeII ACGT 1 cut(s) 615
MaeIII GTNAC 3 cut(s) 49, 451, 517
MalI GATC 2 cut(s) 513, 812
MboI GATC 2 cut(s) 511, 810
MboII GAAGA 3 cut(s) 809, 888, 1123
MfeI CAATTG 1 cut(s) 89
MflI RGATCY 1 cut(s) 511
MhlI GDGCHC 4 cut(s) 40, 384, 615, 1012
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 75, 89, 528, 726, 907, 934
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 985
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 248
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 656, 693, 1053, 1101
MslI CAYNNNNRTG 1 cut(s) 772
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 4 cut(s) 139, 551, 750, 947
MspI CCGG 1 cut(s) 249
MspR9I CCNGG 3 cut(s) 218, 256, 815
MunI CAATTG 1 cut(s) 89
MvaI CCWGG 3 cut(s) 218, 256, 815
NdeI CATATG 1 cut(s) 712
NdeII GATC 2 cut(s) 511, 810
NlaIII CATG 4 cut(s) 10, 293, 929, 1031
NlaIV GGNNCC 5 cut(s) 253, 353, 354, 513, 1059
NmeAIII GCCGAG 1 cut(s) 760
NmuCI GTSAC 2 cut(s) 49, 517
NspI RCATGY 1 cut(s) 293
PaeI GCATGC 1 cut(s) 293
PciSI GCTCTTC 1 cut(s) 1106
PfeI GAWTC 4 cut(s) 169, 298, 415, 1073
PflMI CCANNNNNTGG 3 cut(s) 12, 844, 1028
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 5 cut(s) 141, 180, 733, 946, 949
PmaCI CACGTG 1 cut(s) 616
PmlI CACGTG 1 cut(s) 616
Ppu21I YACGTR 1 cut(s) 616
PpuMI RGGWCCY 1 cut(s) 352
Psp124BI GAGCTC 1 cut(s) 1012
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 3 cut(s) 216, 254, 813
PspCI CACGTG 1 cut(s) 616
PspGI CCWGG 3 cut(s) 216, 254, 813
PspN4I GGNNCC 5 cut(s) 253, 353, 354, 513, 1059
PspPI GGNCC 6 cut(s) 131, 272, 352, 369, 1019, 1034
PspPPI RGGWCCY 1 cut(s) 352
PstNI CAGNNNCTG 1 cut(s) 947
PsuI RGATCY 1 cut(s) 511
PvuII CAGCTG 2 cut(s) 551, 947
RsaI GTAC 3 cut(s) 44, 766, 983
RsaNI GTAC 3 cut(s) 43, 765, 982
RseI CAYNNNNRTG 1 cut(s) 772
SacI GAGCTC 1 cut(s) 1012
SapI GCTCTTC 1 cut(s) 1106
SaqAI TTAA 4 cut(s) 656, 693, 1053, 1101
SatI GCNGC 5 cut(s) 140, 179, 732, 945, 948
Sau3AI GATC 2 cut(s) 511, 810
Sau96I GGNCC 6 cut(s) 131, 272, 352, 369, 1019, 1034
ScrFI CCNGG 3 cut(s) 218, 256, 815
SduI GDGCHC 4 cut(s) 40, 384, 615, 1012
SexAI ACCWGGT 1 cut(s) 254
SfaNI GCATC 3 cut(s) 454, 574, 964
SfcI CTRYAG 1 cut(s) 546
SinI GGWCC 4 cut(s) 272, 352, 1019, 1034
SmiMI CAYNNNNRTG 1 cut(s) 772
SpeI ACTAGT 1 cut(s) 802
SphI GCATGC 1 cut(s) 293
Sse9I AATT 6 cut(s) 75, 89, 528, 726, 907, 934
SsiI CCGC 4 cut(s) 137, 197, 748, 1032
SspMI CTAG 4 cut(s) 114, 366, 560, 803
SstI GAGCTC 1 cut(s) 1012
StyD4I CCNGG 3 cut(s) 216, 254, 813
TaaI ACNGT 6 cut(s) 42, 49, 285, 661, 679, 698
TaiI ACGT 1 cut(s) 618
TaqI TCGA 1 cut(s) 1071
TasI AATT 6 cut(s) 75, 89, 528, 726, 907, 934
TatI WGTACW 1 cut(s) 981
TfiI GAWTC 4 cut(s) 169, 298, 415, 1073
Tru1I TTAA 4 cut(s) 656, 693, 1053, 1101
Tru9I TTAA 4 cut(s) 656, 693, 1053, 1101
TscAI CASTG 5 cut(s) 58, 522, 673, 724, 991
TseFI GTSAC 2 cut(s) 49, 517
TseI GCWGC 5 cut(s) 139, 178, 731, 944, 947
Tsp45I GTSAC 2 cut(s) 49, 517
TspDTI ATGAA 6 cut(s) 307, 311, 901, 947, 1016, 1087
TspGWI ACGGA 1 cut(s) 192
TspRI CASTG 5 cut(s) 58, 522, 673, 724, 991
Van91I CCANNNNNTGG 3 cut(s) 12, 844, 1028
VpaK11BI GGWCC 4 cut(s) 272, 352, 1019, 1034
XagI CCTNNNNNAGG 1 cut(s) 870
XapI RAATTY 2 cut(s) 75, 907
XceI RCATGY 1 cut(s) 293
XspI CTAG 4 cut(s) 114, 366, 560, 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.