RchiOBHm_Chr2g0150821

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003217
Physical Location & Seq
Reverse (-)
68486056 .. 68489405
3350 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52007

Sequence Viewer

Length: 945 bp
ATGGCCATGACTTGGAAGCTCCTGAAGCAACTCCGACCACCACCGTACACGGCCACCGCCTCTACGAGCCTCCACCGCCCTCGATTTCTCAACTTCCAAAATGCCACTCGGCTCTTCTCCGATCAGCCTCCGCTGCCTCCTCCCTCCGGAGAGCCTCCGCCGATTCCACCGCCTCAAAACTCTCACTCTCCCTCGCCGTCGTCGCTGAACCAGGCAGAGCTCGCTAAGTTCGCCGCCATTGCCGGAACCTGGTGGGATTCTGAAGGACCGTTCAAACCTTTGCATGCGATGAATCCTACAAGGCTCGCCTTCATTCGCTCAACGCTGTGTCGTCATTTCAGGAGGGATCCATTATCTGCTAGGCCATTTGAAGGGCTCAAGTTTATTGATGTTGGCTGTGGTGGTGGAATTCTGTCTGAGCCTTTAGCACGCATGGGAGCTTCTGTGACTGGAGTTGATGCTGTTGAGAAAAATATCAAGATAGCTCGCCTTCATGCTGACTTGGATCCGGTGACCTCAAGCATTGAATATCATTGCACAACAGCTGAAAAACTAGTTGACGAGCAGAGGAGTTTTGATGCTGTGTTTGCTTTAGAGGTAATTGAGCACGTGGCGAATCCTGCTGAGTTCTGCAAATCTCTGTCTGCCTTAACTGTTCCTGATGGAGCTACTGTGATTTCAACTATTAACCGTTCTATGAGAGCATATGCTACTGCGATTATTGCTGCCGAGTATCTCCTCCACTGGCTTCCTAAAGGTACACATCAATGGTCCAGTTTCCTTACCCCTGAAGAACTAGTCTTGATCCTGGAGCGTGCTTCTATTTCGGTCCAAGAGATGGCTGGATTTGCATACAACCCTTTGACAGGAAGATGGTCTATATCTGATGATGTTAGTGTAAACTTTATCGCTTATGGTACCAAAACAGAAACAGCCACTTTATAA

Protein Analysis

314

Amino Acids

34.46

Weight (kDa)

6.51

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 114 - 273 1.2e-20 Methyltransferase domain
Methyltransf_31 PF13847 124 - 257 1e-13 Methyltransferase domain
CMAS PF02353 124 - 236 1.6e-06 Mycolic acid cyclopropane synthetase
PrmA PF06325 126 - 229 1.8e-07 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_11 PF08241 129 - 225 6.9e-19 Methyltransferase domain
Methyltransf_25 PF13649 129 - 222 5.1e-15 Methyltransferase domain
Methyltransf_12 PF08242 129 - 222 9.9e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 943
Acc65I GGTACC 1 cut(s) 917
AccB1I GGYRCC 1 cut(s) 917
AccB7I CCANNNNNTGG 2 cut(s) 12, 838
AccIII TCCGGA 1 cut(s) 146
AciI CCGC 6 cut(s) 57, 76, 131, 158, 170, 234
AclWI GGATC 5 cut(s) 341, 354, 500, 513, 799
AcoI YGGCCR 2 cut(s) 3, 51
AcsI RAATTY 1 cut(s) 408
AcuI CTGAAG 3 cut(s) 44, 282, 810
AcvI CACGTG 1 cut(s) 610
AfaI GTAC 3 cut(s) 47, 760, 919
AfiI CCNNNNNNNGG 6 cut(s) 12, 146, 249, 371, 838, 866
AgsI TTSAA 4 cut(s) 274, 371, 527, 681
AhlI ACTAGT 2 cut(s) 553, 796
AjnI CCWGG 3 cut(s) 210, 248, 807
AluBI AGCT 6 cut(s) 19, 220, 440, 485, 545, 668
AluI AGCT 6 cut(s) 19, 220, 440, 485, 545, 668
Alw21I GWGCWC 2 cut(s) 222, 609
AlwI GGATC 5 cut(s) 341, 354, 500, 513, 799
Aor13HI TCCGGA 1 cut(s) 146
AoxI GGCC 3 cut(s) 3, 51, 362
ApeKI GCWGC 2 cut(s) 133, 725
ApoI RAATTY 1 cut(s) 408
Asp718I GGTACC 1 cut(s) 917
AspS9I GGNCC 3 cut(s) 266, 771, 829
AsuHPI GGTGA 1 cut(s) 523
AvaII GGWCC 3 cut(s) 266, 771, 829
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 2 cut(s) 346, 505
BanI GGYRCC 1 cut(s) 917
BanII GRGCYC 2 cut(s) 222, 378
BbrPI CACGTG 1 cut(s) 610
Bbv12I GWGCWC 2 cut(s) 222, 609
BbvI GCAGC 2 cut(s) 120, 712
BccI CCATC 3 cut(s) 656, 832, 867
BceAI ACGGC 2 cut(s) 66, 181
BciT130I CCWGG 3 cut(s) 212, 250, 809
BcuI ACTAGT 2 cut(s) 553, 796
BfaI CTAG 3 cut(s) 360, 554, 797
BisI GCNGC 3 cut(s) 134, 234, 726
BlsI GCNGC 3 cut(s) 135, 235, 727
Bme1390I CCNGG 3 cut(s) 212, 250, 809
Bme18I GGWCC 3 cut(s) 266, 771, 829
BmgT120I GGNCC 3 cut(s) 266, 771, 829
BmiI GGNNCC 4 cut(s) 247, 348, 507, 919
BmrFI CCNGG 3 cut(s) 212, 250, 809
BmsI GCATC 2 cut(s) 448, 568
BpmI CTGGAG 2 cut(s) 471, 830
BpuEI CTTGAG 2 cut(s) 362, 502
BsaAI YACGTR 1 cut(s) 610
BsaWI WCCGGW 2 cut(s) 146, 508
BsaXI ACNNNNNCTCC 4 cut(s) 429, 459, 657, 687
Bsc4I CCNNNNNNNGG 6 cut(s) 12, 146, 249, 371, 838, 866
Bse1I ACTGG 3 cut(s) 454, 749, 774
Bse3DI GCAATG 2 cut(s) 237, 532
BseAI TCCGGA 1 cut(s) 146
BseBI CCWGG 3 cut(s) 212, 250, 809
BseLI CCNNNNNNNGG 6 cut(s) 12, 146, 249, 371, 838, 866
BseMI GCAATG 2 cut(s) 237, 532
BseMII CTCAG 2 cut(s) 408, 615
BseNI ACTGG 3 cut(s) 454, 749, 774
BseRI GAGGAG 3 cut(s) 129, 583, 728
BseXI GCAGC 2 cut(s) 120, 712
BshFI GGCC 3 cut(s) 5, 53, 364
BshNI GGYRCC 1 cut(s) 917
BsiHKAI GWGCWC 2 cut(s) 222, 609
BsiSI CCGG 3 cut(s) 147, 243, 509
BslI CCNNNNNNNGG 6 cut(s) 12, 146, 249, 371, 838, 866
BsnI GGCC 3 cut(s) 5, 53, 364
Bsp1286I GDGCHC 3 cut(s) 222, 378, 609
Bsp13I TCCGGA 1 cut(s) 146
Bsp143I GATC 4 cut(s) 121, 346, 505, 804
BspACI CCGC 6 cut(s) 57, 76, 131, 158, 170, 234
BspANI GGCC 3 cut(s) 5, 53, 364
BspCNI CTCAG 2 cut(s) 409, 616
BspEI TCCGGA 1 cut(s) 146
BspLI GGNNCC 4 cut(s) 247, 348, 507, 919
BspPI GGATC 5 cut(s) 341, 354, 500, 513, 799
BspQI GCTCTTC 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 917
BsrDI GCAATG 2 cut(s) 237, 532
BsrI ACTGG 3 cut(s) 454, 749, 774
BssMI GATC 4 cut(s) 121, 346, 505, 804
Bst2UI CCWGG 3 cut(s) 212, 250, 809
Bst4CI ACNGT 5 cut(s) 45, 270, 655, 673, 692
Bst6I CTCTTC 1 cut(s) 119
BstBAI YACGTR 1 cut(s) 610
BstC8I GCNNGC 6 cut(s) 222, 285, 306, 430, 487, 816
BstDEI CTNAG 3 cut(s) 225, 417, 624
BstEII GGTNACC 1 cut(s) 511
BstENI CCTNNNNNAGG 1 cut(s) 864
BstKTI GATC 4 cut(s) 124, 349, 508, 807
BstMBI GATC 4 cut(s) 121, 346, 505, 804
BstNI CCWGG 3 cut(s) 212, 250, 809
BstNSI RCATGY 1 cut(s) 287
BstPI GGTNACC 1 cut(s) 511
BstSCI CCNGG 3 cut(s) 210, 248, 807
BstV1I GCAGC 2 cut(s) 120, 712
BstX2I RGATCY 2 cut(s) 346, 505
BstYI RGATCY 2 cut(s) 346, 505
BsuRI GGCC 3 cut(s) 5, 53, 364
BtgZI GCGATG 1 cut(s) 302
BtsIMutI CAGTG 1 cut(s) 742
Cac8I GCNNGC 6 cut(s) 222, 285, 306, 430, 487, 816
Cfr13I GGNCC 3 cut(s) 266, 771, 829
CsiI ACCWGGT 1 cut(s) 248
Csp6I GTAC 3 cut(s) 46, 759, 918
CviAII CATG 4 cut(s) 7, 284, 433, 494
CviQI GTAC 3 cut(s) 46, 759, 918
DdeI CTNAG 3 cut(s) 225, 417, 624
DpnI GATC 4 cut(s) 123, 348, 507, 806
DpnII GATC 4 cut(s) 121, 346, 505, 804
EaeI YGGCCR 2 cut(s) 3, 51
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
EciI GGCGGA 1 cut(s) 147
Ecl136II GAGCTC 1 cut(s) 220
Eco24I GRGCYC 2 cut(s) 222, 378
Eco47I GGWCC 3 cut(s) 266, 771, 829
Eco53kI GAGCTC 1 cut(s) 220
Eco57I CTGAAG 3 cut(s) 44, 282, 810
Eco72I CACGTG 1 cut(s) 610
Eco91I GGTNACC 1 cut(s) 511
EcoICRI GAGCTC 1 cut(s) 220
EcoNI CCTNNNNNAGG 1 cut(s) 864
EcoO65I GGTNACC 1 cut(s) 511
EcoRI GAATTC 1 cut(s) 408
EcoRII CCWGG 3 cut(s) 210, 248, 807
EcoT38I GRGCYC 2 cut(s) 222, 378
FaeI CATG 4 cut(s) 10, 287, 436, 497
FatI CATG 4 cut(s) 6, 283, 432, 493
FauNDI CATATG 1 cut(s) 706
Fnu4HI GCNGC 3 cut(s) 134, 234, 726
FriOI GRGCYC 2 cut(s) 222, 378
Fsp4HI GCNGC 3 cut(s) 134, 234, 726
FspBI CTAG 3 cut(s) 360, 554, 797
GluI GCNGC 3 cut(s) 134, 234, 726
GsuI CTGGAG 2 cut(s) 471, 830
HaeIII GGCC 3 cut(s) 5, 53, 364
HapII CCGG 3 cut(s) 147, 243, 509
Hin1II CATG 4 cut(s) 10, 287, 436, 497
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 4 cut(s) 163, 257, 292, 616
HpaII CCGG 3 cut(s) 147, 243, 509
HphI GGTGA 1 cut(s) 523
Hpy166II GTNNAC 4 cut(s) 48, 559, 761, 901
Hpy188I TCNGA 5 cut(s) 35, 121, 262, 418, 886
Hpy188III TCNNGA 6 cut(s) 22, 147, 340, 478, 659, 802
Hpy8I GTNNAC 4 cut(s) 48, 559, 761, 901
Hpy99I CGWCG 2 cut(s) 202, 205
HpyAV CCTTC 4 cut(s) 257, 319, 365, 500
HpyCH4III ACNGT 5 cut(s) 45, 270, 655, 673, 692
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 4 cut(s) 283, 537, 633, 851
HpyF3I CTNAG 3 cut(s) 225, 417, 624
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 4 cut(s) 10, 287, 436, 497
Kpn2I TCCGGA 1 cut(s) 146
KpnI GGTACC 1 cut(s) 921
Kzo9I GATC 4 cut(s) 121, 346, 505, 804
LguI GCTCTTC 1 cut(s) 119
LmnI GCTCC 4 cut(s) 24, 437, 665, 811
Lsp1109I GCAGC 2 cut(s) 120, 712
LweI GCATC 2 cut(s) 448, 568
MabI ACCWGGT 1 cut(s) 248
MaeI CTAG 3 cut(s) 360, 554, 797
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 2 cut(s) 445, 511
MalI GATC 4 cut(s) 123, 348, 507, 806
MboI GATC 4 cut(s) 121, 346, 505, 804
MboII GAAGA 3 cut(s) 106, 803, 882
MflI RGATCY 2 cut(s) 346, 505
MhlI GDGCHC 3 cut(s) 222, 378, 609
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 408, 600
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 58
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 146
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 650, 687
MslI CAYNNNNRTG 1 cut(s) 766
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 133, 545
MspI CCGG 3 cut(s) 147, 243, 509
MspR9I CCNGG 3 cut(s) 212, 250, 809
MvaI CCWGG 3 cut(s) 212, 250, 809
NdeI CATATG 1 cut(s) 706
NdeII GATC 4 cut(s) 121, 346, 505, 804
NlaIII CATG 4 cut(s) 10, 287, 436, 497
NlaIV GGNNCC 4 cut(s) 247, 348, 507, 919
NmeAIII GCCGAG 2 cut(s) 88, 754
NmuCI GTSAC 2 cut(s) 445, 511
NspI RCATGY 1 cut(s) 287
PaeI GCATGC 1 cut(s) 287
PciSI GCTCTTC 1 cut(s) 119
PfeI GAWTC 4 cut(s) 163, 257, 292, 616
PflMI CCANNNNNTGG 2 cut(s) 12, 838
PfoI TCCNGGA 1 cut(s) 807
PkrI GCNGC 3 cut(s) 135, 235, 727
PmaCI CACGTG 1 cut(s) 610
PmlI CACGTG 1 cut(s) 610
Ppu21I YACGTR 1 cut(s) 610
PsiI TTATAA 1 cut(s) 943
Psp124BI GAGCTC 1 cut(s) 222
Psp6I CCWGG 3 cut(s) 210, 248, 807
PspCI CACGTG 1 cut(s) 610
PspEI GGTNACC 1 cut(s) 511
PspGI CCWGG 3 cut(s) 210, 248, 807
PspN4I GGNNCC 4 cut(s) 247, 348, 507, 919
PspPI GGNCC 3 cut(s) 266, 771, 829
PsuI RGATCY 2 cut(s) 346, 505
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 3 cut(s) 47, 760, 919
RsaNI GTAC 3 cut(s) 46, 759, 918
RseI CAYNNNNRTG 1 cut(s) 766
SacI GAGCTC 1 cut(s) 222
SapI GCTCTTC 1 cut(s) 119
SaqAI TTAA 2 cut(s) 650, 687
SatI GCNGC 3 cut(s) 134, 234, 726
Sau3AI GATC 4 cut(s) 121, 346, 505, 804
Sau96I GGNCC 3 cut(s) 266, 771, 829
ScrFI CCNGG 3 cut(s) 212, 250, 809
SduI GDGCHC 3 cut(s) 222, 378, 609
SexAI ACCWGGT 1 cut(s) 248
SfaNI GCATC 2 cut(s) 448, 568
SinI GGWCC 3 cut(s) 266, 771, 829
SmiMI CAYNNNNRTG 1 cut(s) 766
SmlI CTYRAG 2 cut(s) 377, 517
SmoI CTYRAG 2 cut(s) 377, 517
SpeI ACTAGT 2 cut(s) 553, 796
SphI GCATGC 1 cut(s) 287
Sse9I AATT 2 cut(s) 408, 600
SsiI CCGC 6 cut(s) 57, 76, 131, 158, 170, 234
SspMI CTAG 3 cut(s) 360, 554, 797
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 3 cut(s) 210, 248, 807
TaaI ACNGT 5 cut(s) 45, 270, 655, 673, 692
TaiI ACGT 1 cut(s) 612
TaqI TCGA 1 cut(s) 82
TaqII GACCGA 1 cut(s) 817
TasI AATT 2 cut(s) 408, 600
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 4 cut(s) 163, 257, 292, 616
Tru1I TTAA 2 cut(s) 650, 687
Tru9I TTAA 2 cut(s) 650, 687
TscAI CASTG 1 cut(s) 749
TseFI GTSAC 2 cut(s) 445, 511
TseI GCWGC 2 cut(s) 133, 725
Tsp45I GTSAC 2 cut(s) 445, 511
TspDTI ATGAA 3 cut(s) 301, 305, 482
TspRI CASTG 1 cut(s) 749
Van91I CCANNNNNTGG 2 cut(s) 12, 838
VpaK11BI GGWCC 3 cut(s) 266, 771, 829
XagI CCTNNNNNAGG 1 cut(s) 864
XapI RAATTY 1 cut(s) 408
XceI RCATGY 1 cut(s) 287
XcmI CCANNNNNNNNNTGG 1 cut(s) 839
XspI CTAG 3 cut(s) 360, 554, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.