RLG00000005638

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003217
Physical Location & Seq
Forward (+)
501003 .. 504111
3109 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005638

Sequence Viewer

Length: 945 bp
ATGGCCATGACTTGGAAGCTCCTGAAGCAACTCCGACCGCCACCTTACACCGCCACCGCCTCTGCGAGCCTCCACCGCCCTCGATTTCTCAACTTGCATAATGCCACTCGGCTCTTCTCCGATGCGCCTCCGCTGCCTCCTCCCTCCGGAGAGCCTCCGTCGATTCCACCGCCTCAAAACCCTCACTCTCCCTCGCCGTCGTCTCTGAACCAGGCAGAGCTCGCTAAGTTCGCCGCCATTGCCGGAACCTGGTGGGATTCCGAAGGACCATTCAAACCGTTGCATGCCATGAATCCTACAAGACTCGCCTTCATTCGCTCCACGCTGTGTCGTCATTTCAGGAGGGATCCATTATCTGCTACGCCCTTTGAAGGGCTCAAGTTTCTTGATGTTGGCTGTGGCGGTGGAATTCTGTCTGAGCCTTTAGCACGGATGGGAGGTTCTGTAACGGGAGTTGATGCTGTTGATAAAAATATCAAGATAGCTCGCCTTCATGCTGACTTGGATCCGGTGGCCTCAAGCATTGAATATCATTGCACAACAGCTGAGAAACTAGTCGAGGAGCAGAGGAGTTTTGATGCTGTGTTTGCTTTAGAGGTAATTGAGCACGTGGCAAATCCTGCTGAGTTCTGCAAATCTCTGTCTGCCTTAACTGTTCCAGATGGAGCTACCGTGATTTCAACTATTAACCGTTCTATGAGAGCATATGCTACTGCAATTGTTGCTGCCGAGTATCTCCTCCATTGGATTCCTAAAGGTACACATCAATGGTCCAGTTTCCTTACTCCTGAAGAACTAGTCTTGGTCCTGGACCGTGCTTCTATTTCGGTCCAAGAGATGGCTGGATTTGCATACAACCCTTTGACAGGAAGATGGTTTATATCTGACGATGTTAGTGTAAACTTTATCGCTTATGGTACCAAAACAGAAAGAGTCACTTTGTAA

Protein Analysis

315

Amino Acids

34.35

Weight (kDa)

6.55

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 116 - 273 8e-19 Methyltransferase domain
Methyltransf_31 PF13847 124 - 244 1.4e-13 Methyltransferase domain
CMAS PF02353 124 - 238 1e-06 Mycolic acid cyclopropane synthetase
PrmA PF06325 126 - 229 2e-06 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_11 PF08241 129 - 225 3.8e-19 Methyltransferase domain
Methyltransf_25 PF13649 129 - 222 3e-15 Methyltransferase domain
Methyltransf_12 PF08242 129 - 222 6.1e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 917
AccB1I GGYRCC 1 cut(s) 917
AccB7I CCANNNNNTGG 2 cut(s) 12, 838
AccIII TCCGGA 1 cut(s) 146
AciI CCGC 8 cut(s) 38, 51, 57, 76, 131, 170, 234, 402
AclWI GGATC 4 cut(s) 341, 354, 500, 513
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 408
AcuI CTGAAG 2 cut(s) 44, 810
AcvI CACGTG 1 cut(s) 610
AdeI CACNNNGTG 1 cut(s) 327
AfaI GTAC 2 cut(s) 760, 919
AfiI CCNNNNNNNGG 7 cut(s) 12, 146, 249, 371, 372, 838, 866
AgsI TTSAA 4 cut(s) 274, 371, 527, 681
AhlI ACTAGT 2 cut(s) 553, 796
AjnI CCWGG 3 cut(s) 210, 248, 807
AloI GAACNNNNNNTCC 2 cut(s) 424, 456
AluBI AGCT 5 cut(s) 19, 220, 485, 545, 668
AluI AGCT 5 cut(s) 19, 220, 485, 545, 668
Alw21I GWGCWC 2 cut(s) 222, 609
Alw26I GTCTC 1 cut(s) 207
AlwI GGATC 4 cut(s) 341, 354, 500, 513
Aor13HI TCCGGA 1 cut(s) 146
AoxI GGCC 2 cut(s) 3, 513
ApeKI GCWGC 2 cut(s) 133, 725
ApoI RAATTY 1 cut(s) 408
Asp718I GGTACC 1 cut(s) 917
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 5 cut(s) 266, 771, 805, 811, 829
AvaII GGWCC 5 cut(s) 266, 771, 805, 811, 829
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 2 cut(s) 346, 505
BanI GGYRCC 1 cut(s) 917
BanII GRGCYC 2 cut(s) 222, 378
BbrPI CACGTG 1 cut(s) 610
Bbv12I GWGCWC 2 cut(s) 222, 609
BbvI GCAGC 2 cut(s) 120, 712
BccI CCATC 4 cut(s) 427, 656, 832, 867
BceAI ACGGC 1 cut(s) 181
BciT130I CCWGG 3 cut(s) 212, 250, 809
BcoDI GTCTC 1 cut(s) 207
BcuI ACTAGT 2 cut(s) 553, 796
BfaI CTAG 2 cut(s) 554, 797
BisI GCNGC 3 cut(s) 134, 234, 726
BlsI GCNGC 3 cut(s) 135, 235, 727
Bme1390I CCNGG 3 cut(s) 212, 250, 809
Bme18I GGWCC 5 cut(s) 266, 771, 805, 811, 829
BmgT120I GGNCC 5 cut(s) 266, 771, 805, 811, 829
BmiI GGNNCC 4 cut(s) 247, 348, 507, 919
BmrFI CCNGG 3 cut(s) 212, 250, 809
BmsI GCATC 3 cut(s) 112, 448, 568
BpuEI CTTGAG 2 cut(s) 362, 502
BsaAI YACGTR 1 cut(s) 610
BsaWI WCCGGW 2 cut(s) 146, 508
BsaXI ACNNNNNCTCC 4 cut(s) 429, 459, 657, 687
Bsc4I CCNNNNNNNGG 7 cut(s) 12, 146, 249, 371, 372, 838, 866
Bse1I ACTGG 1 cut(s) 774
Bse3DI GCAATG 2 cut(s) 237, 532
BseAI TCCGGA 1 cut(s) 146
BseBI CCWGG 3 cut(s) 212, 250, 809
BseGI GGATG 1 cut(s) 438
BseLI CCNNNNNNNGG 7 cut(s) 12, 146, 249, 371, 372, 838, 866
BseMI GCAATG 2 cut(s) 237, 532
BseMII CTCAG 3 cut(s) 408, 537, 615
BseNI ACTGG 1 cut(s) 774
BseRI GAGGAG 4 cut(s) 129, 575, 583, 728
BseXI GCAGC 2 cut(s) 120, 712
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 2 cut(s) 5, 515
BshNI GGYRCC 1 cut(s) 917
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 222, 609
BsiSI CCGG 3 cut(s) 147, 243, 509
BslI CCNNNNNNNGG 7 cut(s) 12, 146, 249, 371, 372, 838, 866
BsmAI GTCTC 1 cut(s) 207
BsmBI CGTCTC 1 cut(s) 207
BsnI GGCC 2 cut(s) 5, 515
Bsp1286I GDGCHC 3 cut(s) 222, 378, 609
Bsp13I TCCGGA 1 cut(s) 146
Bsp143I GATC 2 cut(s) 346, 505
BspACI CCGC 8 cut(s) 38, 51, 57, 76, 131, 170, 234, 402
BspANI GGCC 2 cut(s) 5, 515
BspCNI CTCAG 3 cut(s) 409, 538, 616
BspEI TCCGGA 1 cut(s) 146
BspLI GGNNCC 4 cut(s) 247, 348, 507, 919
BspPI GGATC 4 cut(s) 341, 354, 500, 513
BspQI GCTCTTC 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 917
BsrDI GCAATG 2 cut(s) 237, 532
BsrI ACTGG 1 cut(s) 774
BssMI GATC 2 cut(s) 346, 505
Bst2UI CCWGG 3 cut(s) 212, 250, 809
Bst4CI ACNGT 5 cut(s) 279, 655, 673, 692, 815
Bst6I CTCTTC 1 cut(s) 119
BstAPI GCANNNNNTGC 2 cut(s) 620, 722
BstBAI YACGTR 1 cut(s) 610
BstC8I GCNNGC 4 cut(s) 67, 222, 285, 487
BstDEI CTNAG 4 cut(s) 225, 417, 546, 624
BstENI CCTNNNNNAGG 1 cut(s) 864
BstF5I GGATG 1 cut(s) 438
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 2 cut(s) 349, 508
BstMAI GTCTC 1 cut(s) 207
BstMBI GATC 2 cut(s) 346, 505
BstMCI CGRYCG 1 cut(s) 38
BstNI CCWGG 3 cut(s) 212, 250, 809
BstNSI RCATGY 1 cut(s) 287
BstSCI CCNGG 3 cut(s) 210, 248, 807
BstV1I GCAGC 2 cut(s) 120, 712
BstX2I RGATCY 2 cut(s) 346, 505
BstYI RGATCY 2 cut(s) 346, 505
BsuRI GGCC 2 cut(s) 5, 515
BtsCI GGATG 1 cut(s) 438
Cac8I GCNNGC 4 cut(s) 67, 222, 285, 487
CfoI GCGC 1 cut(s) 127
Cfr13I GGNCC 5 cut(s) 266, 771, 805, 811, 829
CsiI ACCWGGT 1 cut(s) 248
Csp6I GTAC 2 cut(s) 759, 918
CviAII CATG 4 cut(s) 7, 284, 289, 494
CviQI GTAC 2 cut(s) 759, 918
DdeI CTNAG 4 cut(s) 225, 417, 546, 624
DpnI GATC 2 cut(s) 348, 507
DpnII GATC 2 cut(s) 346, 505
DraIII CACNNNGTG 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Ecl136II GAGCTC 1 cut(s) 220
Eco24I GRGCYC 2 cut(s) 222, 378
Eco47I GGWCC 5 cut(s) 266, 771, 805, 811, 829
Eco53kI GAGCTC 1 cut(s) 220
Eco57I CTGAAG 2 cut(s) 44, 810
Eco72I CACGTG 1 cut(s) 610
EcoICRI GAGCTC 1 cut(s) 220
EcoNI CCTNNNNNAGG 1 cut(s) 864
EcoRI GAATTC 1 cut(s) 408
EcoRII CCWGG 3 cut(s) 210, 248, 807
EcoT38I GRGCYC 2 cut(s) 222, 378
Esp3I CGTCTC 1 cut(s) 207
FaeI CATG 4 cut(s) 10, 287, 292, 497
FalI AAGNNNNNCTT 1 cut(s) 922
FatI CATG 4 cut(s) 6, 283, 288, 493
FauNDI CATATG 1 cut(s) 706
Fnu4HI GCNGC 3 cut(s) 134, 234, 726
FokI GGATG 1 cut(s) 445
FriOI GRGCYC 2 cut(s) 222, 378
Fsp4HI GCNGC 3 cut(s) 134, 234, 726
FspBI CTAG 2 cut(s) 554, 797
GlaI GCGC 1 cut(s) 126
GluI GCNGC 3 cut(s) 134, 234, 726
HaeIII GGCC 2 cut(s) 5, 515
HapII CCGG 3 cut(s) 147, 243, 509
HhaI GCGC 1 cut(s) 127
Hin1II CATG 4 cut(s) 10, 287, 292, 497
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HinfI GANTC 6 cut(s) 163, 257, 292, 303, 748, 933
HpaII CCGG 3 cut(s) 147, 243, 509
Hpy166II GTNNAC 2 cut(s) 761, 901
Hpy188I TCNGA 6 cut(s) 35, 121, 207, 262, 418, 886
Hpy188III TCNNGA 7 cut(s) 22, 147, 340, 386, 478, 659, 788
Hpy8I GTNNAC 2 cut(s) 761, 901
Hpy99I CGWCG 2 cut(s) 163, 202
HpyAV CCTTC 4 cut(s) 257, 319, 365, 500
HpyCH4III ACNGT 5 cut(s) 279, 655, 673, 692, 815
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 6 cut(s) 97, 283, 537, 633, 716, 851
HpyF3I CTNAG 4 cut(s) 225, 417, 546, 624
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 4 cut(s) 10, 287, 292, 497
HspAI GCGC 1 cut(s) 125
Kpn2I TCCGGA 1 cut(s) 146
KpnI GGTACC 1 cut(s) 921
Kzo9I GATC 2 cut(s) 346, 505
LguI GCTCTTC 1 cut(s) 119
LmnI GCTCC 4 cut(s) 24, 323, 562, 665
Lsp1109I GCAGC 2 cut(s) 120, 712
LweI GCATC 3 cut(s) 112, 448, 568
MabI ACCWGGT 1 cut(s) 248
MaeI CTAG 2 cut(s) 554, 797
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 2 cut(s) 445, 934
MalI GATC 2 cut(s) 348, 507
MboI GATC 2 cut(s) 346, 505
MboII GAAGA 3 cut(s) 106, 803, 882
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 2 cut(s) 346, 505
MhlI GDGCHC 3 cut(s) 222, 378, 609
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 408, 600, 717
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 297, 942
MmeI TCCRAC 1 cut(s) 58
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 146
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 650, 687
MslI CAYNNNNRTG 1 cut(s) 766
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 133, 545
MspI CCGG 3 cut(s) 147, 243, 509
MspR9I CCNGG 3 cut(s) 212, 250, 809
MunI CAATTG 1 cut(s) 717
MvaI CCWGG 3 cut(s) 212, 250, 809
NdeI CATATG 1 cut(s) 706
NdeII GATC 2 cut(s) 346, 505
NlaIII CATG 4 cut(s) 10, 287, 292, 497
NlaIV GGNNCC 4 cut(s) 247, 348, 507, 919
NmeAIII GCCGAG 2 cut(s) 88, 754
NmuCI GTSAC 1 cut(s) 934
NspI RCATGY 1 cut(s) 287
PaeI GCATGC 1 cut(s) 287
PciSI GCTCTTC 1 cut(s) 119
PfeI GAWTC 4 cut(s) 163, 257, 292, 748
PflMI CCANNNNNTGG 2 cut(s) 12, 838
PfoI TCCNGGA 1 cut(s) 807
PkrI GCNGC 3 cut(s) 135, 235, 727
PleI GAGTC 2 cut(s) 297, 941
PmaCI CACGTG 1 cut(s) 610
PmlI CACGTG 1 cut(s) 610
PpsI GAGTC 2 cut(s) 297, 941
Ppu21I YACGTR 1 cut(s) 610
Psp124BI GAGCTC 1 cut(s) 222
Psp6I CCWGG 3 cut(s) 210, 248, 807
PspCI CACGTG 1 cut(s) 610
PspGI CCWGG 3 cut(s) 210, 248, 807
PspN4I GGNNCC 4 cut(s) 247, 348, 507, 919
PspPI GGNCC 5 cut(s) 266, 771, 805, 811, 829
PsuI RGATCY 2 cut(s) 346, 505
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 2 cut(s) 760, 919
RsaNI GTAC 2 cut(s) 759, 918
RseI CAYNNNNRTG 1 cut(s) 766
SacI GAGCTC 1 cut(s) 222
SapI GCTCTTC 1 cut(s) 119
SaqAI TTAA 2 cut(s) 650, 687
SatI GCNGC 3 cut(s) 134, 234, 726
Sau3AI GATC 2 cut(s) 346, 505
Sau96I GGNCC 5 cut(s) 266, 771, 805, 811, 829
SchI GAGTC 2 cut(s) 297, 942
ScrFI CCNGG 3 cut(s) 212, 250, 809
SduI GDGCHC 3 cut(s) 222, 378, 609
SexAI ACCWGGT 1 cut(s) 248
SfaNI GCATC 3 cut(s) 112, 448, 568
SinI GGWCC 5 cut(s) 266, 771, 805, 811, 829
SmiMI CAYNNNNRTG 1 cut(s) 766
SmlI CTYRAG 2 cut(s) 377, 517
SmoI CTYRAG 2 cut(s) 377, 517
SpeI ACTAGT 2 cut(s) 553, 796
SphI GCATGC 1 cut(s) 287
Sse9I AATT 3 cut(s) 408, 600, 717
SsiI CCGC 8 cut(s) 38, 51, 57, 76, 131, 170, 234, 402
SspMI CTAG 2 cut(s) 554, 797
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 3 cut(s) 210, 248, 807
TaaI ACNGT 5 cut(s) 279, 655, 673, 692, 815
TaiI ACGT 1 cut(s) 612
TaqI TCGA 3 cut(s) 82, 161, 558
TaqII GACCGA 1 cut(s) 817
TasI AATT 3 cut(s) 408, 600, 717
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 4 cut(s) 163, 257, 292, 748
Tru1I TTAA 2 cut(s) 650, 687
Tru9I TTAA 2 cut(s) 650, 687
TseFI GTSAC 1 cut(s) 934
TseI GCWGC 2 cut(s) 133, 725
Tsp45I GTSAC 1 cut(s) 934
TspDTI ATGAA 3 cut(s) 301, 305, 482
TspGWI ACGGA 2 cut(s) 147, 445
Van91I CCANNNNNTGG 2 cut(s) 12, 838
VpaK11BI GGWCC 5 cut(s) 266, 771, 805, 811, 829
XagI CCTNNNNNAGG 1 cut(s) 864
XapI RAATTY 1 cut(s) 408
XceI RCATGY 1 cut(s) 287
XcmI CCANNNNNNNNNTGG 1 cut(s) 839
XspI CTAG 2 cut(s) 554, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.