MD17G1142800.v1.1

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003217
Physical Location & Seq
Forward (+)
12880775 .. 12884176
3402 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1142800.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGCTGTGGCTTTGAAGCTCCTGAAGCAACTCCGAGCACCGCACACCAGCTCCACAATCCTCCACCGCAATCTCAGCCCACGGCTCTCGAGCATTCACAGTGCAACCAGACTCTTCTCAGACTCTCCTCCTCTAACCGAGCCTCTTCCGGCTCCACCGCCGCTAAACCCTCATTCTCCTTCCCCATCGTCTCTGAACCAGTACGAGCTCGCCAAGTTCGCCGCCGTTGCCGAAACCTGGTGGGATTCTGAAGGGCCGTTCAAACCATTGCATATGCTGAATCCTACAAGACTTGCTTTCATTCGCTCCACGCTTTGCCGACATTTCGGGAAGGATCCGTTGTCCCCTAAGCCATTCGAAGGTCTCAAATTTATTGATGTTGGCTGCGGTGGTGGAATTCTTTCTGAGCCTTTAGCTCGAATGGGAGCTACTGTGACGGGAGTTGATGCAGTGGAGAAAAATATCAACATAGCGCGCGTTCACGCTGATTTAGATCCAGTGACTTCGAGAATTGACTATCAATGCACAACAGCTGAAAAGCTAGTTGAGGAACAGAAGAATTTTGATGCTGTGTTTGCTTTGGAGGTAATTGAGCACGTTGCAGACCCTACTGAGTTCTGCAAATCTCTATCAGCCTTAACTGTCCCCGGTGGAGCTACTGTTGTTTCAACTATTAACCGTTCTATGAGAGCGTATGCTGCTGCCATTGTTGCAGCAGAGTATATCCTCCACTGGCTTCCTAAAGGTACACATCAATATTCCAGTTTTCTCACTCCCGAAGAACTAGCCCTGATCCTGGAACGTGCTTCTATTTCAGTCCAAGAGATGGCTGGGATCGAGTACAATCCTTTGACAGGAAGATGGTCTCTATCTGATGATGTCAGTGTAAATTTTATTGCTTATGGTATCAAAACAGTGACATCCAGTTTATAA

Protein Analysis

311

Amino Acids

33.8

Weight (kDa)

6.2

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 112 - 269 1.8e-21 Methyltransferase domain
Methyltransf_31 PF13847 120 - 236 8.1e-14 Methyltransferase domain
CMAS PF02353 120 - 234 3e-06 Mycolic acid cyclopropane synthetase
PrmA PF06325 123 - 225 2.7e-07 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_11 PF08241 125 - 221 5.9e-19 Methyltransferase domain
Methyltransf_25 PF13649 125 - 218 2e-15 Methyltransferase domain
Methyltransf_12 PF08242 125 - 218 2.5e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 931
AccB7I CCANNNNNTGG 1 cut(s) 826
AccII CGCG 2 cut(s) 475, 477
AciI CCGC 6 cut(s) 41, 67, 158, 161, 222, 387
AclWI GGATC 5 cut(s) 329, 342, 488, 787, 842
AcsI RAATTY 4 cut(s) 368, 396, 559, 889
AcuI CTGAAG 2 cut(s) 44, 270
AfaI GTAC 3 cut(s) 203, 748, 842
AfiI CCNNNNNNNGG 5 cut(s) 237, 359, 796, 826, 854
AgsI TTSAA 3 cut(s) 16, 262, 669
AjnI CCWGG 2 cut(s) 236, 795
AluBI AGCT 8 cut(s) 19, 51, 208, 416, 428, 533, 541, 656
AluI AGCT 8 cut(s) 19, 51, 208, 416, 428, 533, 541, 656
Alw21I GWGCWC 3 cut(s) 40, 210, 597
Alw26I GTCTC 3 cut(s) 195, 368, 870
AlwI GGATC 5 cut(s) 329, 342, 488, 787, 842
Ama87I CYCGRG 1 cut(s) 88
AoxI GGCC 1 cut(s) 254
ApeKI GCWGC 4 cut(s) 384, 698, 701, 713
ApoI RAATTY 4 cut(s) 368, 396, 559, 889
Asp700I GAANNNNTTC 1 cut(s) 400
AspLEI GCGC 2 cut(s) 475, 477
AspS9I GGNCC 1 cut(s) 254
AsuC2I CCSGG 1 cut(s) 648
AsuII TTCGAA 1 cut(s) 357
AvaI CYCGRG 1 cut(s) 88
BamHI GGATCC 1 cut(s) 334
BanII GRGCYC 1 cut(s) 210
Bbv12I GWGCWC 3 cut(s) 40, 210, 597
BbvI GCAGC 4 cut(s) 371, 685, 688, 725
BccI CCATC 3 cut(s) 193, 820, 855
BceAI ACGGC 3 cut(s) 98, 209, 241
BciT130I CCWGG 2 cut(s) 238, 797
BcnI CCSGG 1 cut(s) 648
BcoDI GTCTC 3 cut(s) 195, 368, 870
BfaI CTAG 2 cut(s) 542, 785
BisI GCNGC 6 cut(s) 161, 222, 385, 699, 702, 714
BlsI GCNGC 6 cut(s) 162, 223, 386, 700, 703, 715
Bme1390I CCNGG 3 cut(s) 238, 648, 797
BmeT110I CYCGRG 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 2 cut(s) 153, 336
BmrFI CCNGG 3 cut(s) 238, 648, 797
BmsI GCATC 2 cut(s) 436, 556
Bpu10I CCTNAGC 1 cut(s) 348
Bpu14I TTCGAA 1 cut(s) 357
BpuMI CCSGG 1 cut(s) 648
BsaBI GATNNNNATC 1 cut(s) 492
BsaI GGTCTC 2 cut(s) 368, 870
BsaJI CCNNGG 2 cut(s) 80, 646
BsaXI ACNNNNNCTCC 6 cut(s) 35, 65, 417, 447, 645, 675
Bsc4I CCNNNNNNNGG 5 cut(s) 237, 359, 796, 826, 854
Bse1I ACTGG 5 cut(s) 199, 497, 737, 762, 924
Bse3DI GCAATG 1 cut(s) 266
Bse8I GATNNNNATC 1 cut(s) 492
BseBI CCWGG 2 cut(s) 238, 797
BseDI CCNNGG 2 cut(s) 80, 646
BseGI GGATG 1 cut(s) 920
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 5 cut(s) 237, 359, 796, 826, 854
BseMI GCAATG 1 cut(s) 266
BseMII CTCAG 4 cut(s) 88, 132, 396, 603
BseNI ACTGG 5 cut(s) 199, 497, 737, 762, 924
BsePI GCGCGC 1 cut(s) 473
BseRI GAGGAG 2 cut(s) 117, 120
BseXI GCAGC 4 cut(s) 371, 685, 688, 725
BseYI CCCAGC 1 cut(s) 830
Bsh1236I CGCG 2 cut(s) 475, 477
BshFI GGCC 1 cut(s) 256
BsiHKAI GWGCWC 3 cut(s) 40, 210, 597
BsiHKCI CYCGRG 1 cut(s) 88
BsiSI CCGG 2 cut(s) 149, 648
BslFI GGGAC 2 cut(s) 328, 629
BslI CCNNNNNNNGG 5 cut(s) 237, 359, 796, 826, 854
BsmAI GTCTC 3 cut(s) 195, 368, 870
BsmBI CGTCTC 1 cut(s) 195
BsmFI GGGAC 2 cut(s) 328, 629
BsmI GAATGC 1 cut(s) 93
BsnI GGCC 1 cut(s) 256
Bso31I GGTCTC 2 cut(s) 368, 870
BsoBI CYCGRG 1 cut(s) 88
Bsp119I TTCGAA 1 cut(s) 357
Bsp1286I GDGCHC 3 cut(s) 40, 210, 597
Bsp143I GATC 4 cut(s) 334, 493, 792, 834
BspACI CCGC 6 cut(s) 41, 67, 158, 161, 222, 387
BspANI GGCC 1 cut(s) 256
BspCNI CTCAG 4 cut(s) 87, 131, 397, 604
BspFNI CGCG 2 cut(s) 475, 477
BspLI GGNNCC 2 cut(s) 153, 336
BspPI GGATC 5 cut(s) 329, 342, 488, 787, 842
BspT104I TTCGAA 1 cut(s) 357
BspTNI GGTCTC 2 cut(s) 368, 870
BsrDI GCAATG 1 cut(s) 266
BsrI ACTGG 5 cut(s) 199, 497, 737, 762, 924
BssECI CCNNGG 2 cut(s) 80, 646
BssHII GCGCGC 1 cut(s) 473
BssMI GATC 4 cut(s) 334, 493, 792, 834
Bst2UI CCWGG 2 cut(s) 238, 797
Bst4CI ACNGT 6 cut(s) 101, 433, 643, 661, 680, 916
Bst6I CTCTTC 2 cut(s) 119, 150
BstBI TTCGAA 1 cut(s) 357
BstC8I GCNNGC 2 cut(s) 210, 475
BstDEI CTNAG 5 cut(s) 74, 118, 348, 405, 612
BstDSI CCRYGG 1 cut(s) 80
BstENI CCTNNNNNAGG 1 cut(s) 852
BstF5I GGATG 1 cut(s) 920
BstFNI CGCG 2 cut(s) 475, 477
BstHHI GCGC 2 cut(s) 475, 477
BstKTI GATC 4 cut(s) 337, 496, 795, 837
BstMAI GTCTC 3 cut(s) 195, 368, 870
BstMBI GATC 4 cut(s) 334, 493, 792, 834
BstMWI GCNNNNNNNGC 7 cut(s) 25, 75, 218, 227, 575, 698, 710
BstNI CCWGG 2 cut(s) 238, 797
BstSCI CCNGG 3 cut(s) 236, 646, 795
BstUI CGCG 2 cut(s) 475, 477
BstV1I GCAGC 4 cut(s) 371, 685, 688, 725
BstX2I RGATCY 2 cut(s) 334, 493
BstYI RGATCY 2 cut(s) 334, 493
BsuRI GGCC 1 cut(s) 256
BtgI CCRYGG 1 cut(s) 80
BtsCI GGATG 1 cut(s) 920
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 6 cut(s) 106, 456, 504, 730, 889, 921
Cac8I GCNNGC 2 cut(s) 210, 475
CfoI GCGC 2 cut(s) 475, 477
Cfr13I GGNCC 1 cut(s) 254
CsiI ACCWGGT 1 cut(s) 236
Csp6I GTAC 3 cut(s) 202, 747, 841
CviQI GTAC 3 cut(s) 202, 747, 841
DdeI CTNAG 5 cut(s) 74, 118, 348, 405, 612
DpnI GATC 4 cut(s) 336, 495, 794, 836
DpnII GATC 4 cut(s) 334, 493, 792, 834
Eam1104I CTCTTC 2 cut(s) 119, 150
EarI CTCTTC 2 cut(s) 119, 150
Ecl136II GAGCTC 1 cut(s) 208
Eco24I GRGCYC 1 cut(s) 210
Eco31I GGTCTC 2 cut(s) 368, 870
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 2 cut(s) 44, 270
Eco88I CYCGRG 1 cut(s) 88
EcoICRI GAGCTC 1 cut(s) 208
EcoNI CCTNNNNNAGG 1 cut(s) 852
EcoRI GAATTC 1 cut(s) 396
EcoRII CCWGG 2 cut(s) 236, 795
EcoT38I GRGCYC 1 cut(s) 210
Esp3I CGTCTC 1 cut(s) 195
FaiI YATR 8 cut(s) 273, 275, 470, 686, 696, 723, 903, 931
FalI AAGNNNNNCTT 2 cut(s) 280, 312
FaqI GGGAC 2 cut(s) 328, 629
FauNDI CATATG 1 cut(s) 273
Fnu4HI GCNGC 6 cut(s) 161, 222, 385, 699, 702, 714
FokI GGATG 1 cut(s) 907
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 6 cut(s) 161, 222, 385, 699, 702, 714
FspBI CTAG 2 cut(s) 542, 785
GlaI GCGC 2 cut(s) 474, 476
GluI GCNGC 6 cut(s) 161, 222, 385, 699, 702, 714
GsaI CCCAGC 1 cut(s) 834
HaeIII GGCC 1 cut(s) 256
HapII CCGG 2 cut(s) 149, 648
HhaI GCGC 2 cut(s) 475, 477
Hin6I GCGC 2 cut(s) 473, 475
HinP1I GCGC 2 cut(s) 473, 475
HinfI GANTC 4 cut(s) 111, 122, 245, 280
HpaII CCGG 2 cut(s) 149, 648
Hpy166II GTNNAC 2 cut(s) 481, 749
Hpy188I TCNGA 6 cut(s) 35, 121, 195, 250, 406, 874
Hpy188III TCNNGA 5 cut(s) 22, 88, 328, 507, 776
Hpy8I GTNNAC 2 cut(s) 481, 749
HpyAV CCTTC 4 cut(s) 189, 245, 325, 353
HpyCH4III ACNGT 6 cut(s) 101, 433, 643, 661, 680, 916
HpyCH4IV ACGT 2 cut(s) 597, 802
HpyCH4V TGCA 7 cut(s) 104, 271, 449, 525, 602, 621, 713
HpyF10VI GCNNNNNNNGC 7 cut(s) 25, 75, 218, 227, 575, 698, 710
HpyF3I CTNAG 5 cut(s) 74, 118, 348, 405, 612
HpySE526I ACGT 2 cut(s) 597, 802
HspAI GCGC 2 cut(s) 473, 475
Kzo9I GATC 4 cut(s) 334, 493, 792, 834
LmnI GCTCC 6 cut(s) 24, 56, 157, 311, 425, 653
Lsp1109I GCAGC 4 cut(s) 371, 685, 688, 725
LweI GCATC 2 cut(s) 436, 556
MabI ACCWGGT 1 cut(s) 236
MaeI CTAG 2 cut(s) 542, 785
MaeII ACGT 2 cut(s) 597, 802
MaeIII GTNAC 3 cut(s) 433, 499, 916
MalI GATC 4 cut(s) 336, 495, 794, 836
MboI GATC 4 cut(s) 334, 493, 792, 834
MboII GAAGA 5 cut(s) 106, 137, 568, 791, 870
MflI RGATCY 2 cut(s) 334, 493
MhlI GDGCHC 3 cut(s) 40, 210, 597
MluCI AATT 6 cut(s) 368, 396, 510, 559, 588, 889
MlyI GAGTC 2 cut(s) 105, 116
MnlI CCTC 8 cut(s) 71, 138, 141, 153, 180, 541, 577, 737
MroXI GAANNNNTTC 1 cut(s) 400
MseI TTAA 2 cut(s) 638, 675
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 2 cut(s) 149, 648
MspR9I CCNGG 3 cut(s) 238, 648, 797
Mva1269I GAATGC 1 cut(s) 93
MvaI CCWGG 2 cut(s) 238, 797
MvnI CGCG 2 cut(s) 475, 477
MwoI GCNNNNNNNGC 7 cut(s) 25, 75, 218, 227, 575, 698, 710
NciI CCSGG 1 cut(s) 648
NdeI CATATG 1 cut(s) 273
NdeII GATC 4 cut(s) 334, 493, 792, 834
NlaIV GGNNCC 2 cut(s) 153, 336
NmuCI GTSAC 3 cut(s) 433, 499, 916
NspV TTCGAA 1 cut(s) 357
PaeR7I CTCGAG 1 cut(s) 88
PauI GCGCGC 1 cut(s) 473
PctI GAATGC 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 400
PfeI GAWTC 2 cut(s) 245, 280
PflMI CCANNNNNTGG 1 cut(s) 826
PfoI TCCNGGA 1 cut(s) 795
PkrI GCNGC 6 cut(s) 162, 223, 386, 700, 703, 715
PleI GAGTC 2 cut(s) 105, 116
PpsI GAGTC 2 cut(s) 105, 116
PsiI TTATAA 1 cut(s) 931
Psp124BI GAGCTC 1 cut(s) 210
Psp6I CCWGG 2 cut(s) 236, 795
PspFI CCCAGC 1 cut(s) 830
PspGI CCWGG 2 cut(s) 236, 795
PspN4I GGNNCC 2 cut(s) 153, 336
PspPI GGNCC 1 cut(s) 254
PsuI RGATCY 2 cut(s) 334, 493
PteI GCGCGC 1 cut(s) 473
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 3 cut(s) 203, 748, 842
RsaNI GTAC 3 cut(s) 202, 747, 841
SacI GAGCTC 1 cut(s) 210
SaqAI TTAA 2 cut(s) 638, 675
SatI GCNGC 6 cut(s) 161, 222, 385, 699, 702, 714
Sau3AI GATC 4 cut(s) 334, 493, 792, 834
Sau96I GGNCC 1 cut(s) 254
SchI GAGTC 2 cut(s) 105, 116
ScrFI CCNGG 3 cut(s) 238, 648, 797
SduI GDGCHC 3 cut(s) 40, 210, 597
SexAI ACCWGGT 1 cut(s) 236
SfaNI GCATC 2 cut(s) 436, 556
Sfr274I CTCGAG 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 357
SlaI CTCGAG 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
Sse9I AATT 6 cut(s) 368, 396, 510, 559, 588, 889
SsiI CCGC 6 cut(s) 41, 67, 158, 161, 222, 387
SspI AATATT 1 cut(s) 758
SspMI CTAG 2 cut(s) 542, 785
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 3 cut(s) 236, 646, 795
TaaI ACNGT 6 cut(s) 101, 433, 643, 661, 680, 916
TaiI ACGT 2 cut(s) 600, 805
TaqI TCGA 5 cut(s) 89, 357, 418, 506, 837
TasI AATT 6 cut(s) 368, 396, 510, 559, 588, 889
TatI WGTACW 1 cut(s) 840
TauI GCSGC 2 cut(s) 163, 224
TfiI GAWTC 2 cut(s) 245, 280
Tru1I TTAA 2 cut(s) 638, 675
Tru9I TTAA 2 cut(s) 638, 675
TscAI CASTG 6 cut(s) 106, 456, 504, 737, 889, 921
TseFI GTSAC 3 cut(s) 433, 499, 916
TseI GCWGC 4 cut(s) 384, 698, 701, 713
Tsp45I GTSAC 3 cut(s) 433, 499, 916
TspDTI ATGAA 1 cut(s) 289
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 6 cut(s) 106, 456, 504, 737, 889, 921
Van91I CCANNNNNTGG 1 cut(s) 826
XagI CCTNNNNNAGG 1 cut(s) 852
XapI RAATTY 4 cut(s) 368, 396, 559, 889
XcmI CCANNNNNNNNNTGG 1 cut(s) 827
XhoI CTCGAG 1 cut(s) 88
XmnI GAANNNNTTC 1 cut(s) 400
XspI CTAG 2 cut(s) 542, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.