Rh4DG443200

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003217
Physical Location & Seq
Reverse (-)
64432244 .. 64435322
3079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG443200.1

Sequence Viewer

Length: 933 bp
ATGGCTGGGAAGCTCCTGAAGCAGCTCCGACCGCCACCGTACACCGCCACCGCCTCTGCGAGCCTCCACCGCCCTCGATTTCTCAACTTCCATAATGCCACTCGGCTCTTCTCCGATGCGCCTCCTCCCTCCGGAGAGCCTCCGCCGTCGATTCCACCGCCTCAAAACCCTCACTCTCCCTCGCCGTCGTCTCTGAACCAGGCAGAGCTCGCTAAGTTCGCCGCCATTGCCGGAACCTGGTGGGATTCTGAAGGACCATTCAAACCTTTGCATGCCATGAATCCTACAAGACTCGCCTTCATTCGCTCCACGCTGTGTCGTCATTTCAGGAGGGATCCATTATCTGCTAGGCCCTTTGAAGGGCTCAAGTTTCTTGATGTTGGCTGTGGTGGTGGAATTCTGTCTGAGCCTTTAGCACGGATGGGAGCTTCTGTAACGGGAGTTGATGCTGTGGATAAAAATATCAAGATAGCTCGCCTTCATGCTGACTTGGATCCGGTGACCTCAAGCATTGAATATCATTGCACAACAGCTGAAAAACTAGTCGAGGAGCAGAGGAGTTTTGATGCTGTGTTTGCTTTAGAGGTAATTGAGCACGTGGCAAATCCTGCTGAGTTCTGCAAATCTCTGTCTGCCTTAACTGTTCCTGATGGAGCTAGCGTGATTTCAACTATTAACCGTTCTATGAGAGCATATGCTACTGCAATTGTTGCTGCCGAGTATCTCCTCCATTGGCTTCCTAAAGGTACACATCAATGGTCCAGTTTCCTTACTCCTGAAGAACTAGTCTTGATCCTGGAGCGTGCTTCTATTTCGGTCCAAGAGATGGCTGGATTTGCATACAACCCTTTGACAGGAAGATGGTTTATATCTGACGATGTTAGTGTAAACTTTATCGCTTATGGTACCAAAATAGAAACAGTCACTTTATAA

Protein Analysis

310

Amino Acids

33.89

Weight (kDa)

6.55

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 110 - 269 7.9e-21 Methyltransferase domain
Methyltransf_31 PF13847 120 - 251 1.4e-13 Methyltransferase domain
CMAS PF02353 120 - 232 5.6e-07 Mycolic acid cyclopropane synthetase
PrmA PF06325 122 - 225 3.8e-07 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_11 PF08241 125 - 221 2.5e-19 Methyltransferase domain
Methyltransf_25 PF13649 125 - 218 2e-15 Methyltransferase domain
Methyltransf_12 PF08242 125 - 218 5.1e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 931
Acc65I GGTACC 1 cut(s) 905
AccB1I GGYRCC 1 cut(s) 905
AccB7I CCANNNNNTGG 1 cut(s) 826
AccIII TCCGGA 1 cut(s) 131
AciI CCGC 7 cut(s) 32, 45, 51, 70, 143, 158, 222
AclWI GGATC 5 cut(s) 329, 342, 488, 501, 787
AcsI RAATTY 1 cut(s) 396
AcuI CTGAAG 3 cut(s) 38, 270, 798
AcvI CACGTG 1 cut(s) 598
AdeI CACNNNGTG 1 cut(s) 315
AfaI GTAC 3 cut(s) 41, 748, 907
AfiI CCNNNNNNNGG 6 cut(s) 131, 237, 359, 360, 826, 854
AgsI TTSAA 4 cut(s) 262, 359, 515, 669
AhlI ACTAGT 2 cut(s) 541, 784
AjnI CCWGG 3 cut(s) 198, 236, 795
AluBI AGCT 7 cut(s) 13, 25, 208, 428, 473, 533, 656
AluI AGCT 7 cut(s) 13, 25, 208, 428, 473, 533, 656
Alw21I GWGCWC 2 cut(s) 210, 597
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 5 cut(s) 329, 342, 488, 501, 787
Aor13HI TCCGGA 1 cut(s) 131
AoxI GGCC 1 cut(s) 350
ApeKI GCWGC 2 cut(s) 22, 713
ApoI RAATTY 1 cut(s) 396
Asp718I GGTACC 1 cut(s) 905
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 4 cut(s) 254, 351, 759, 817
AsuHPI GGTGA 1 cut(s) 511
AsuNHI GCTAGC 1 cut(s) 656
AvaII GGWCC 3 cut(s) 254, 759, 817
BamHI GGATCC 2 cut(s) 334, 493
BanI GGYRCC 1 cut(s) 905
BanII GRGCYC 2 cut(s) 210, 366
BbrPI CACGTG 1 cut(s) 598
Bbv12I GWGCWC 2 cut(s) 210, 597
BbvI GCAGC 2 cut(s) 34, 700
BccI CCATC 4 cut(s) 415, 644, 820, 855
BceAI ACGGC 2 cut(s) 130, 169
BciT130I CCWGG 3 cut(s) 200, 238, 797
BcoDI GTCTC 1 cut(s) 195
BcuI ACTAGT 2 cut(s) 541, 784
BfaI CTAG 4 cut(s) 348, 542, 657, 785
BisI GCNGC 3 cut(s) 23, 222, 714
BlsI GCNGC 3 cut(s) 24, 223, 715
Bme1390I CCNGG 3 cut(s) 200, 238, 797
Bme18I GGWCC 3 cut(s) 254, 759, 817
BmgT120I GGNCC 4 cut(s) 254, 351, 759, 817
BmiI GGNNCC 4 cut(s) 235, 336, 495, 907
BmrFI CCNGG 3 cut(s) 200, 238, 797
BmsI GCATC 3 cut(s) 106, 436, 556
BmtI GCTAGC 1 cut(s) 660
BpmI CTGGAG 1 cut(s) 818
BpuEI CTTGAG 2 cut(s) 350, 490
BsaAI YACGTR 1 cut(s) 598
BsaWI WCCGGW 2 cut(s) 131, 496
BsaXI ACNNNNNCTCC 4 cut(s) 417, 447, 645, 675
Bsc4I CCNNNNNNNGG 6 cut(s) 131, 237, 359, 360, 826, 854
Bse1I ACTGG 1 cut(s) 762
Bse3DI GCAATG 2 cut(s) 225, 520
BseAI TCCGGA 1 cut(s) 131
BseBI CCWGG 3 cut(s) 200, 238, 797
BseGI GGATG 1 cut(s) 426
BseLI CCNNNNNNNGG 6 cut(s) 131, 237, 359, 360, 826, 854
BseMI GCAATG 2 cut(s) 225, 520
BseMII CTCAG 2 cut(s) 396, 603
BseNI ACTGG 1 cut(s) 762
BseRI GAGGAG 4 cut(s) 114, 563, 571, 716
BseXI GCAGC 2 cut(s) 34, 700
BseYI CCCAGC 1 cut(s) 5
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 1 cut(s) 352
BshNI GGYRCC 1 cut(s) 905
BsiEI CGRYCG 1 cut(s) 32
BsiHKAI GWGCWC 2 cut(s) 210, 597
BsiSI CCGG 3 cut(s) 132, 231, 497
BslI CCNNNNNNNGG 6 cut(s) 131, 237, 359, 360, 826, 854
BsmAI GTCTC 1 cut(s) 195
BsmBI CGTCTC 1 cut(s) 195
BsnI GGCC 1 cut(s) 352
Bsp1286I GDGCHC 3 cut(s) 210, 366, 597
Bsp13I TCCGGA 1 cut(s) 131
Bsp143I GATC 3 cut(s) 334, 493, 792
BspACI CCGC 7 cut(s) 32, 45, 51, 70, 143, 158, 222
BspANI GGCC 1 cut(s) 352
BspCNI CTCAG 2 cut(s) 397, 604
BspEI TCCGGA 1 cut(s) 131
BspLI GGNNCC 4 cut(s) 235, 336, 495, 907
BspOI GCTAGC 1 cut(s) 660
BspPI GGATC 5 cut(s) 329, 342, 488, 501, 787
BspQI GCTCTTC 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 905
BsrDI GCAATG 2 cut(s) 225, 520
BsrI ACTGG 1 cut(s) 762
BssMI GATC 3 cut(s) 334, 493, 792
Bst2UI CCWGG 3 cut(s) 200, 238, 797
Bst4CI ACNGT 4 cut(s) 39, 643, 680, 922
Bst6I CTCTTC 1 cut(s) 113
BstAPI GCANNNNNTGC 2 cut(s) 608, 710
BstBAI YACGTR 1 cut(s) 598
BstC8I GCNNGC 6 cut(s) 61, 210, 273, 475, 658, 804
BstDEI CTNAG 3 cut(s) 213, 405, 612
BstEII GGTNACC 1 cut(s) 499
BstENI CCTNNNNNAGG 1 cut(s) 852
BstF5I GGATG 1 cut(s) 426
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 3 cut(s) 337, 496, 795
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 3 cut(s) 334, 493, 792
BstMCI CGRYCG 1 cut(s) 32
BstNI CCWGG 3 cut(s) 200, 238, 797
BstNSI RCATGY 1 cut(s) 275
BstPI GGTNACC 1 cut(s) 499
BstSCI CCNGG 3 cut(s) 198, 236, 795
BstV1I GCAGC 2 cut(s) 34, 700
BstX2I RGATCY 2 cut(s) 334, 493
BstYI RGATCY 2 cut(s) 334, 493
BsuRI GGCC 1 cut(s) 352
BtsCI GGATG 1 cut(s) 426
Cac8I GCNNGC 6 cut(s) 61, 210, 273, 475, 658, 804
CfoI GCGC 1 cut(s) 121
Cfr13I GGNCC 4 cut(s) 254, 351, 759, 817
CsiI ACCWGGT 1 cut(s) 236
Csp6I GTAC 3 cut(s) 40, 747, 906
CviAII CATG 3 cut(s) 272, 277, 482
CviQI GTAC 3 cut(s) 40, 747, 906
DdeI CTNAG 3 cut(s) 213, 405, 612
DpnI GATC 3 cut(s) 336, 495, 794
DpnII GATC 3 cut(s) 334, 493, 792
DraIII CACNNNGTG 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 1 cut(s) 132
Ecl136II GAGCTC 1 cut(s) 208
Eco24I GRGCYC 2 cut(s) 210, 366
Eco47I GGWCC 3 cut(s) 254, 759, 817
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 3 cut(s) 38, 270, 798
Eco72I CACGTG 1 cut(s) 598
Eco91I GGTNACC 1 cut(s) 499
EcoICRI GAGCTC 1 cut(s) 208
EcoNI CCTNNNNNAGG 1 cut(s) 852
EcoO109I RGGNCCY 1 cut(s) 351
EcoO65I GGTNACC 1 cut(s) 499
EcoRI GAATTC 1 cut(s) 396
EcoRII CCWGG 3 cut(s) 198, 236, 795
EcoT38I GRGCYC 2 cut(s) 210, 366
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 3 cut(s) 275, 280, 485
FatI CATG 3 cut(s) 271, 276, 481
FauNDI CATATG 1 cut(s) 694
Fnu4HI GCNGC 3 cut(s) 23, 222, 714
FokI GGATG 1 cut(s) 433
FriOI GRGCYC 2 cut(s) 210, 366
Fsp4HI GCNGC 3 cut(s) 23, 222, 714
FspBI CTAG 4 cut(s) 348, 542, 657, 785
GlaI GCGC 1 cut(s) 120
GluI GCNGC 3 cut(s) 23, 222, 714
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 818
HaeIII GGCC 1 cut(s) 352
HapII CCGG 3 cut(s) 132, 231, 497
HhaI GCGC 1 cut(s) 121
Hin1II CATG 3 cut(s) 275, 280, 485
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HinfI GANTC 4 cut(s) 151, 245, 280, 291
HpaII CCGG 3 cut(s) 132, 231, 497
HphI GGTGA 1 cut(s) 511
Hpy166II GTNNAC 3 cut(s) 42, 749, 889
Hpy188I TCNGA 6 cut(s) 29, 115, 195, 250, 406, 874
Hpy188III TCNNGA 8 cut(s) 16, 132, 328, 374, 466, 647, 776, 790
Hpy8I GTNNAC 3 cut(s) 42, 749, 889
Hpy99I CGWCG 2 cut(s) 151, 190
HpyAV CCTTC 4 cut(s) 245, 307, 353, 488
HpyCH4III ACNGT 4 cut(s) 39, 643, 680, 922
HpyCH4IV ACGT 1 cut(s) 597
HpyCH4V TGCA 5 cut(s) 271, 525, 621, 704, 839
HpyF3I CTNAG 3 cut(s) 213, 405, 612
HpySE526I ACGT 1 cut(s) 597
Hsp92II CATG 3 cut(s) 275, 280, 485
HspAI GCGC 1 cut(s) 119
Kpn2I TCCGGA 1 cut(s) 131
KpnI GGTACC 1 cut(s) 909
Kzo9I GATC 3 cut(s) 334, 493, 792
LguI GCTCTTC 1 cut(s) 113
LmnI GCTCC 7 cut(s) 18, 30, 311, 425, 550, 653, 799
Lsp1109I GCAGC 2 cut(s) 34, 700
LweI GCATC 3 cut(s) 106, 436, 556
MabI ACCWGGT 1 cut(s) 236
MaeI CTAG 4 cut(s) 348, 542, 657, 785
MaeII ACGT 1 cut(s) 597
MaeIII GTNAC 3 cut(s) 433, 499, 922
MalI GATC 3 cut(s) 336, 495, 794
MboI GATC 3 cut(s) 334, 493, 792
MboII GAAGA 3 cut(s) 100, 791, 870
MfeI CAATTG 1 cut(s) 705
MflI RGATCY 2 cut(s) 334, 493
MhlI GDGCHC 3 cut(s) 210, 366, 597
MluCI AATT 3 cut(s) 396, 588, 705
MlyI GAGTC 1 cut(s) 285
MmeI TCCRAC 1 cut(s) 52
MroI TCCGGA 1 cut(s) 131
MseI TTAA 2 cut(s) 638, 675
MslI CAYNNNNRTG 1 cut(s) 754
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 3 cut(s) 132, 231, 497
MspR9I CCNGG 3 cut(s) 200, 238, 797
MunI CAATTG 1 cut(s) 705
MvaI CCWGG 3 cut(s) 200, 238, 797
NdeI CATATG 1 cut(s) 694
NdeII GATC 3 cut(s) 334, 493, 792
NheI GCTAGC 1 cut(s) 656
NlaIII CATG 3 cut(s) 275, 280, 485
NlaIV GGNNCC 4 cut(s) 235, 336, 495, 907
NmeAIII GCCGAG 2 cut(s) 82, 742
NmuCI GTSAC 2 cut(s) 499, 922
NspI RCATGY 1 cut(s) 275
PaeI GCATGC 1 cut(s) 275
PciSI GCTCTTC 1 cut(s) 113
PfeI GAWTC 3 cut(s) 151, 245, 280
PflMI CCANNNNNTGG 1 cut(s) 826
PfoI TCCNGGA 1 cut(s) 795
PkrI GCNGC 3 cut(s) 24, 223, 715
PleI GAGTC 1 cut(s) 285
PmaCI CACGTG 1 cut(s) 598
PmlI CACGTG 1 cut(s) 598
PpsI GAGTC 1 cut(s) 285
Ppu21I YACGTR 1 cut(s) 598
PsiI TTATAA 1 cut(s) 931
Psp124BI GAGCTC 1 cut(s) 210
Psp6I CCWGG 3 cut(s) 198, 236, 795
PspCI CACGTG 1 cut(s) 598
PspEI GGTNACC 1 cut(s) 499
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 3 cut(s) 198, 236, 795
PspN4I GGNNCC 4 cut(s) 235, 336, 495, 907
PspPI GGNCC 4 cut(s) 254, 351, 759, 817
PsuI RGATCY 2 cut(s) 334, 493
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 3 cut(s) 41, 748, 907
RsaNI GTAC 3 cut(s) 40, 747, 906
RseI CAYNNNNRTG 1 cut(s) 754
SacI GAGCTC 1 cut(s) 210
SapI GCTCTTC 1 cut(s) 113
SaqAI TTAA 2 cut(s) 638, 675
SatI GCNGC 3 cut(s) 23, 222, 714
Sau3AI GATC 3 cut(s) 334, 493, 792
Sau96I GGNCC 4 cut(s) 254, 351, 759, 817
SchI GAGTC 1 cut(s) 285
ScrFI CCNGG 3 cut(s) 200, 238, 797
SduI GDGCHC 3 cut(s) 210, 366, 597
SexAI ACCWGGT 1 cut(s) 236
SfaNI GCATC 3 cut(s) 106, 436, 556
SinI GGWCC 3 cut(s) 254, 759, 817
SmiMI CAYNNNNRTG 1 cut(s) 754
SmlI CTYRAG 2 cut(s) 365, 505
SmoI CTYRAG 2 cut(s) 365, 505
SpeI ACTAGT 2 cut(s) 541, 784
SphI GCATGC 1 cut(s) 275
Sse9I AATT 3 cut(s) 396, 588, 705
SsiI CCGC 7 cut(s) 32, 45, 51, 70, 143, 158, 222
SspMI CTAG 4 cut(s) 348, 542, 657, 785
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 3 cut(s) 198, 236, 795
TaaI ACNGT 4 cut(s) 39, 643, 680, 922
TaiI ACGT 1 cut(s) 600
TaqI TCGA 3 cut(s) 76, 149, 546
TaqII GACCGA 1 cut(s) 805
TasI AATT 3 cut(s) 396, 588, 705
TauI GCSGC 1 cut(s) 224
TfiI GAWTC 3 cut(s) 151, 245, 280
Tru1I TTAA 2 cut(s) 638, 675
Tru9I TTAA 2 cut(s) 638, 675
TseFI GTSAC 2 cut(s) 499, 922
TseI GCWGC 2 cut(s) 22, 713
Tsp45I GTSAC 2 cut(s) 499, 922
TspDTI ATGAA 3 cut(s) 289, 293, 470
TspGWI ACGGA 1 cut(s) 433
Van91I CCANNNNNTGG 1 cut(s) 826
VpaK11BI GGWCC 3 cut(s) 254, 759, 817
XagI CCTNNNNNAGG 1 cut(s) 852
XapI RAATTY 1 cut(s) 396
XceI RCATGY 1 cut(s) 275
XcmI CCANNNNNNNNNTGG 1 cut(s) 827
XspI CTAG 4 cut(s) 348, 542, 657, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.