MD09G1208000.v1.1

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003380
Physical Location & Seq
Reverse (-)
19937208 .. 19940388
3181 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1208000.v1.1.491

Sequence Viewer

Length: 966 bp
ATGTCTATGCCACTGGAGCATGATTACATAGGCCTAACAGAGACTTCATCTTCTCCAATGGAAAGAAGCTCTGAGAAGATTTCATCTTCCACTTCCTCCGCCATCAACCTCAAGGAGACTGAGCTCAGGCTTGGCCTGCCTGGGTCCCACTCTCCTGACAGAAAAAAACCATCAGGGCTTGGACTTGGGGTCTCAATTTTTGGGAAAGATTTGGAGGAGAATAACAAGCCAACTGGATTTTCTCCAAACCCTCCAAAGAACCCTGTGTCAGGGGCAAAAAGGGGATTCTCTGACGCCATTGATGGGTGTTCTGAGAAATGGGCTTTTACCATGACTAATGGATCTGAGGTTGATGTGGGTAAAGCGGCTGTCTTGGGTTCTCCATTAGCCAAGAAGGAGGTTTCTTCTGTTCCTCTGTCGCCAAAGCCAGCAGCTCAGTTGGAGAAGAAGAACACTCAGGCCTCTGAGCATGCTGCTGCTTCTCCTTCTTGCAAGGCACAGGTGGTAGGATGGCCCCCAATTCGATCATTTCGGAAGAATTCCATGGCCTCTAATCTGGCGAAAAACAACGATGACGCTGCGGGCAAACAAGGGTCCGGGTGCCTTTATGTGAAGGTTAGCATGGACGGCGCTCCATACCTGCGAAAAGTTGACCTTAAAACCTACAACAACTACACTGAACTTTCCATGGCTCTGGAGAAGATGTTTAGCTGCTTCAACATTGGGCAGTGCAGTTCTAATGGACTTCCAGAGCGAGATGGTCTGAGTGCGAGTCGATTGATGGATCTTCTCAATGGTTCTGAATTTGTTCTGACTTACGAGGACAAGGATGATGACTGGATGCTTGTTGGCGATGTTCCTTGGCAGATGTTCACTGAGACCTGTAGGAGGCTGAGGATCATGAAAGGTTCTGAAGCAATCGGACTAGCTCCAAGGGCCGCGGGGAAGTGCAAGAACCGCAACTAA

Protein Analysis

322

Amino Acids

34.61

Weight (kDa)

8.56

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 39 - 312 1.7e-88 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 600
AccII CGCG 1 cut(s) 941
AciI CCGC 6 cut(s) 99, 365, 581, 939, 941, 958
AclWI GGATC 3 cut(s) 349, 792, 905
AcsI RAATTY 2 cut(s) 538, 803
AcuI CTGAAG 1 cut(s) 933
AcyI GRCGYC 1 cut(s) 294
AfiI CCNNNNNNNGG 3 cut(s) 269, 303, 888
AgsI TTSAA 1 cut(s) 718
AhdI GACNNNNNGTC 1 cut(s) 188
AjnI CCWGG 1 cut(s) 139
AjuI GAANNNNNNNTTGG 2 cut(s) 223, 255
AluBI AGCT 5 cut(s) 69, 124, 434, 711, 929
AluI AGCT 5 cut(s) 69, 124, 434, 711, 929
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 4 cut(s) 35, 110, 196, 872
AlwI GGATC 3 cut(s) 349, 792, 905
AoxI GGCC 6 cut(s) 31, 133, 459, 512, 546, 936
ApeKI GCWGC 5 cut(s) 431, 473, 476, 578, 711
ApoI RAATTY 2 cut(s) 538, 803
Asp700I GAANNNNTTC 1 cut(s) 807
AspLEI GCGC 1 cut(s) 632
AspS9I GGNCC 4 cut(s) 144, 513, 594, 936
AsuC2I CCSGG 1 cut(s) 598
AvaII GGWCC 2 cut(s) 144, 594
BanI GGYRCC 1 cut(s) 600
BanII GRGCYC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 893
BbvI GCAGC 5 cut(s) 443, 460, 463, 565, 698
BccI CCATC 6 cut(s) 110, 178, 296, 504, 752, 775
BceAI ACGGC 1 cut(s) 643
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 1 cut(s) 141
BcnI CCSGG 1 cut(s) 598
BcoDI GTCTC 4 cut(s) 35, 110, 196, 872
BfaI CTAG 1 cut(s) 926
BfmI CTRYAG 1 cut(s) 883
BfoI RGCGCY 1 cut(s) 633
BfuAI ACCTGC 1 cut(s) 648
BisI GCNGC 7 cut(s) 366, 432, 474, 477, 579, 712, 939
BlsI GCNGC 7 cut(s) 367, 433, 475, 478, 580, 713, 940
Bme1390I CCNGG 2 cut(s) 141, 598
Bme18I GGWCC 2 cut(s) 144, 594
BmeRI GACNNNNNGTC 1 cut(s) 188
BmgT120I GGNCC 4 cut(s) 144, 513, 594, 936
BmiI GGNNCC 5 cut(s) 145, 146, 515, 595, 602
BmrFI CCNGG 2 cut(s) 141, 598
BmsI GCATC 1 cut(s) 831
BpmI CTGGAG 2 cut(s) 35, 716
Bpu10I CCTNAGC 2 cut(s) 125, 893
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 598
BsaHI GRCGYC 1 cut(s) 294
BsaI GGTCTC 2 cut(s) 196, 872
BsaJI CCNNGG 6 cut(s) 140, 543, 687, 860, 932, 939
BsaXI ACNNNNNCTCC 2 cut(s) 689, 719
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 303, 888
Bse1I ACTGG 3 cut(s) 18, 238, 842
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 6 cut(s) 140, 543, 687, 860, 932, 939
BseGI GGATG 3 cut(s) 515, 835, 846
BseLI CCNNNNNNNGG 3 cut(s) 269, 303, 888
BseNI ACTGG 3 cut(s) 18, 238, 842
BseRI GAGGAG 1 cut(s) 230
BseXI GCAGC 5 cut(s) 443, 460, 463, 565, 698
BsgI GTGCAG 1 cut(s) 751
Bsh1236I CGCG 1 cut(s) 941
BshFI GGCC 6 cut(s) 33, 135, 461, 514, 548, 938
BshNI GGYRCC 1 cut(s) 600
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 1 cut(s) 597
BslFI GGGAC 1 cut(s) 130
BslI CCNNNNNNNGG 3 cut(s) 269, 303, 888
BsmAI GTCTC 4 cut(s) 35, 110, 196, 872
BsmFI GGGAC 1 cut(s) 130
BsnI GGCC 6 cut(s) 33, 135, 461, 514, 548, 938
Bso31I GGTCTC 2 cut(s) 196, 872
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 4 cut(s) 341, 524, 784, 897
Bsp19I CCATGG 2 cut(s) 543, 687
BspACI CCGC 6 cut(s) 99, 365, 581, 939, 941, 958
BspANI GGCC 6 cut(s) 33, 135, 461, 514, 548, 938
BspFNI CGCG 1 cut(s) 941
BspHI TCATGA 1 cut(s) 900
BspLI GGNNCC 5 cut(s) 145, 146, 515, 595, 602
BspMI ACCTGC 1 cut(s) 648
BspPI GGATC 3 cut(s) 349, 792, 905
BspT107I GGYRCC 1 cut(s) 600
BspTNI GGTCTC 2 cut(s) 196, 872
BsrI ACTGG 3 cut(s) 18, 238, 842
BssECI CCNNGG 6 cut(s) 140, 543, 687, 860, 932, 939
BssMI GATC 4 cut(s) 341, 524, 784, 897
BssNI GRCGYC 1 cut(s) 294
BssT1I CCWWGG 4 cut(s) 543, 687, 860, 932
Bst2UI CCWGG 1 cut(s) 141
BstACI GRCGYC 1 cut(s) 294
BstC8I GCNNGC 4 cut(s) 137, 429, 471, 583
BstDSI CCRYGG 3 cut(s) 543, 687, 939
BstENI CCTNNNNNAGG 2 cut(s) 267, 886
BstF5I GGATG 3 cut(s) 515, 835, 846
BstFNI CGCG 1 cut(s) 941
BstH2I RGCGCY 1 cut(s) 633
BstHHI GCGC 1 cut(s) 632
BstKTI GATC 4 cut(s) 344, 527, 787, 900
BstMAI GTCTC 4 cut(s) 35, 110, 196, 872
BstMBI GATC 4 cut(s) 341, 524, 784, 897
BstMWI GCNNNNNNNGC 5 cut(s) 16, 136, 627, 935, 957
BstNI CCWGG 1 cut(s) 141
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 139, 596
BstSFI CTRYAG 1 cut(s) 883
BstUI CGCG 1 cut(s) 941
BstV1I GCAGC 5 cut(s) 443, 460, 463, 565, 698
BstX2I RGATCY 2 cut(s) 341, 784
BstXI CCANNNNNNTGG 1 cut(s) 694
BstYI RGATCY 2 cut(s) 341, 784
BsuRI GGCC 6 cut(s) 33, 135, 461, 514, 548, 938
BtgI CCRYGG 3 cut(s) 543, 687, 939
BtgZI GCGATG 1 cut(s) 867
BtsCI GGATG 3 cut(s) 515, 835, 846
BtsI GCAGTG 1 cut(s) 734
BtsIMutI CAGTG 4 cut(s) 11, 675, 734, 873
BveI ACCTGC 1 cut(s) 648
Cac8I GCNNGC 4 cut(s) 137, 429, 471, 583
CciI TCATGA 1 cut(s) 900
CfoI GCGC 1 cut(s) 632
Cfr13I GGNCC 4 cut(s) 144, 513, 594, 936
Cfr42I CCGCGG 1 cut(s) 942
CseI GACGC 2 cut(s) 302, 584
CviAII CATG 7 cut(s) 20, 331, 470, 544, 622, 688, 901
DpnI GATC 4 cut(s) 343, 526, 786, 899
DpnII GATC 4 cut(s) 341, 524, 784, 897
DriI GACNNNNNGTC 1 cut(s) 188
Eam1105I GACNNNNNGTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 88
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 4 cut(s) 543, 687, 860, 932
Eco147I AGGCCT 2 cut(s) 33, 461
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 2 cut(s) 196, 872
Eco47I GGWCC 2 cut(s) 144, 594
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 933
EcoICRI GAGCTC 1 cut(s) 124
EcoNI CCTNNNNNAGG 2 cut(s) 267, 886
EcoO109I RGGNCCY 1 cut(s) 144
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 139
EcoT14I CCWWGG 4 cut(s) 543, 687, 860, 932
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 4 cut(s) 543, 687, 860, 932
FaeI CATG 7 cut(s) 23, 334, 473, 547, 625, 691, 904
FalI AAGNNNNNCTT 2 cut(s) 639, 671
FaqI GGGAC 1 cut(s) 130
FatI CATG 7 cut(s) 19, 330, 469, 543, 621, 687, 900
FauI CCCGC 2 cut(s) 574, 934
Fnu4HI GCNGC 7 cut(s) 366, 432, 474, 477, 579, 712, 939
FokI GGATG 3 cut(s) 522, 842, 853
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 7 cut(s) 366, 432, 474, 477, 579, 712, 939
FspBI CTAG 1 cut(s) 926
GlaI GCGC 1 cut(s) 631
GluI GCNGC 7 cut(s) 366, 432, 474, 477, 579, 712, 939
GsuI CTGGAG 2 cut(s) 35, 716
HaeII RGCGCY 1 cut(s) 633
HaeIII GGCC 6 cut(s) 33, 135, 461, 514, 548, 938
HapII CCGG 1 cut(s) 597
HgaI GACGC 2 cut(s) 302, 584
HhaI GCGC 1 cut(s) 632
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 7 cut(s) 23, 334, 473, 547, 625, 691, 904
Hin6I GCGC 1 cut(s) 630
HinP1I GCGC 1 cut(s) 630
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HinfI GANTC 2 cut(s) 285, 772
HpaII CCGG 1 cut(s) 597
Hpy166II GTNNAC 2 cut(s) 652, 873
Hpy188III TCNNGA 4 cut(s) 155, 695, 749, 901
Hpy8I GTNNAC 2 cut(s) 652, 873
HpyAV CCTTC 3 cut(s) 388, 495, 607
HpyCH4V TGCA 3 cut(s) 492, 732, 951
HpyF10VI GCNNNNNNNGC 5 cut(s) 16, 136, 627, 935, 957
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 7 cut(s) 23, 334, 473, 547, 625, 691, 904
HspAI GCGC 1 cut(s) 630
KflI GGGWCCC 1 cut(s) 144
KspI CCGCGG 1 cut(s) 942
Kzo9I GATC 4 cut(s) 341, 524, 784, 897
LmnI GCTCC 3 cut(s) 16, 637, 934
Lsp1109I GCAGC 5 cut(s) 443, 460, 463, 565, 698
LweI GCATC 1 cut(s) 831
MaeI CTAG 1 cut(s) 926
MalI GATC 4 cut(s) 343, 526, 786, 899
MboI GATC 4 cut(s) 341, 524, 784, 897
MboII GAAGA 9 cut(s) 42, 78, 88, 396, 457, 460, 547, 712, 779
MflI RGATCY 2 cut(s) 341, 784
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 4 cut(s) 195, 519, 538, 803
MlyI GAGTC 1 cut(s) 781
MmeI TCCRAC 1 cut(s) 420
MroXI GAANNNNTTC 1 cut(s) 807
MseI TTAA 1 cut(s) 657
MspA1I CMGCKG 1 cut(s) 941
MspI CCGG 1 cut(s) 597
MspR9I CCNGG 2 cut(s) 141, 598
MvaI CCWGG 1 cut(s) 141
MvnI CGCG 1 cut(s) 941
MwoI GCNNNNNNNGC 5 cut(s) 16, 136, 627, 935, 957
NciI CCSGG 1 cut(s) 598
NcoI CCATGG 2 cut(s) 543, 687
NdeII GATC 4 cut(s) 341, 524, 784, 897
NlaIII CATG 7 cut(s) 23, 334, 473, 547, 625, 691, 904
NlaIV GGNNCC 5 cut(s) 145, 146, 515, 595, 602
NspI RCATGY 1 cut(s) 473
PaeI GCATGC 1 cut(s) 473
PagI TCATGA 1 cut(s) 900
PceI AGGCCT 2 cut(s) 33, 461
PdmI GAANNNNTTC 1 cut(s) 807
PfeI GAWTC 1 cut(s) 285
PkrI GCNGC 7 cut(s) 367, 433, 475, 478, 580, 713, 940
PleI GAGTC 1 cut(s) 780
PpsI GAGTC 1 cut(s) 780
PpuMI RGGWCCY 1 cut(s) 144
Psp124BI GAGCTC 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 5 cut(s) 145, 146, 515, 595, 602
PspPI GGNCC 4 cut(s) 144, 513, 594, 936
PspPPI RGGWCCY 1 cut(s) 144
PsuI RGATCY 2 cut(s) 341, 784
SacI GAGCTC 1 cut(s) 126
SacII CCGCGG 1 cut(s) 942
SaqAI TTAA 1 cut(s) 657
SatI GCNGC 7 cut(s) 366, 432, 474, 477, 579, 712, 939
Sau3AI GATC 4 cut(s) 341, 524, 784, 897
Sau96I GGNCC 4 cut(s) 144, 513, 594, 936
SchI GAGTC 1 cut(s) 781
ScrFI CCNGG 2 cut(s) 141, 598
SduI GDGCHC 1 cut(s) 126
SfaNI GCATC 1 cut(s) 831
SfcI CTRYAG 1 cut(s) 883
Sfr303I CCGCGG 1 cut(s) 942
SgrBI CCGCGG 1 cut(s) 942
SinI GGWCC 2 cut(s) 144, 594
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
SphI GCATGC 1 cut(s) 473
Sse9I AATT 4 cut(s) 195, 519, 538, 803
SseBI AGGCCT 2 cut(s) 33, 461
SsiI CCGC 6 cut(s) 99, 365, 581, 939, 941, 958
SspMI CTAG 1 cut(s) 926
SstI GAGCTC 1 cut(s) 126
StuI AGGCCT 2 cut(s) 33, 461
StyD4I CCNGG 2 cut(s) 139, 596
StyI CCWWGG 4 cut(s) 543, 687, 860, 932
TaqI TCGA 2 cut(s) 523, 775
TasI AATT 4 cut(s) 195, 519, 538, 803
TauI GCSGC 2 cut(s) 368, 941
TfiI GAWTC 1 cut(s) 285
Tru1I TTAA 1 cut(s) 657
Tru9I TTAA 1 cut(s) 657
TscAI CASTG 4 cut(s) 18, 682, 734, 880
TseI GCWGC 5 cut(s) 431, 473, 476, 578, 711
TspDTI ATGAA 3 cut(s) 36, 72, 917
TspRI CASTG 4 cut(s) 18, 682, 734, 880
VpaK11BI GGWCC 2 cut(s) 144, 594
XagI CCTNNNNNAGG 2 cut(s) 267, 886
XapI RAATTY 2 cut(s) 538, 803
XceI RCATGY 1 cut(s) 473
XmnI GAANNNNTTC 1 cut(s) 807
XspI CTAG 1 cut(s) 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.