Rh2CG061400

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003380
Physical Location & Seq
Forward (+)
5009383 .. 5013067
3685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG061400.1

Sequence Viewer

Length: 711 bp
ATGTCTATGTCCTTGGAGCATGATTACATAGGCTTATCTGAGTCTGTTCCTTCAATGGAAGCCTCTGACAGGTCTAACAAGGGAGCTGCTTTGAACCTCAAAGCCACTGAGCTCAGGCTGGGCTTGCCTGGGTCTGAGTCCCCAGAGAGAGATAGTGGTGGTGTGGAGGACAAAACTGGGTTTTCACTGCCTGTGCCTAAACCCTCTGTGTCCGGAGCCAAGAGGGTGTTCTCTGACGCCATTGATGGAGCTTCTGGGAAGTGGGTTTTCTCTGGGAGTGGTGGATCTGAGGCTGATTTGGGTAAAGGTGGTAACTTGGTTTCTCCTAGAGGTGTTAATGGTGGAAAAGGTCTTGCTGGGCCTGAGTGTAAAAGTCAACCAATCTCTGCAGTGAAAGATGGGGTACAACAGTCCCCAAACCCTTTGCATGAGAAGAAGCCTCAGGTTTCTGCTCCTGCAGCAAAGGCACAGGTTGTTGGATGGCCACCAATTCGTTCATTCCGGAAGAATTCAATGGCATCCATTCCTCCCAAAAAGGATGATGATGCAGAAGGCAAGATCGGAGCAGGGTGTCTTTATATCAAGGTCAGCATGGATGGTGCACCATACCTGAGGAAAGTCGATCTCAAAACCTATGGTAGCTATGTGGATCTATCATTAGCACTGGAGAAGATGTTCAGCTGCTTTACAATTGGTAGATTCTCTTTTTAA

Protein Analysis

236

Amino Acids

24.72

Weight (kDa)

8.86

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 35 - 234 1.3e-44 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 212, 501
AclWI GGATC 2 cut(s) 292, 657
AcoI YGGCCR 1 cut(s) 482
AcsI RAATTY 1 cut(s) 508
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 1 cut(s) 405
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 3 cut(s) 54, 94, 513
AjnI CCWGG 1 cut(s) 127
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AloI GAACNNNNNNTCC 2 cut(s) 659, 691
AluBI AGCT 5 cut(s) 86, 112, 251, 642, 681
AluI AGCT 5 cut(s) 86, 112, 251, 642, 681
Alw21I GWGCWC 2 cut(s) 114, 604
Alw44I GTGCAC 1 cut(s) 600
AlwI GGATC 2 cut(s) 292, 657
Aor13HI TCCGGA 2 cut(s) 212, 501
AoxI GGCC 2 cut(s) 359, 482
ApaLI GTGCAC 1 cut(s) 600
ApeKI GCWGC 3 cut(s) 86, 458, 681
ApoI RAATTY 1 cut(s) 508
Asp700I GAANNNNTTC 1 cut(s) 674
AspS9I GGNCC 1 cut(s) 359
AxyI CCTNAGG 2 cut(s) 441, 611
BaeGI GKGCMC 1 cut(s) 604
BaeI ACNNNNGTAYC 2 cut(s) 395, 428
BalI TGGCCA 1 cut(s) 484
BanII GRGCYC 1 cut(s) 114
Bbv12I GWGCWC 2 cut(s) 114, 604
BbvI GCAGC 3 cut(s) 73, 470, 668
BccI CCATC 4 cut(s) 239, 392, 474, 590
BciT130I CCWGG 1 cut(s) 129
BfaI CTAG 1 cut(s) 327
BfmI CTRYAG 2 cut(s) 387, 456
BisI GCNGC 3 cut(s) 87, 459, 682
BlsI GCNGC 3 cut(s) 88, 460, 683
Bme1390I CCNGG 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 359
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 1 cut(s) 129
BmrI ACTGGG 1 cut(s) 186
BmsI GCATC 2 cut(s) 527, 535
BmuI ACTGGG 1 cut(s) 186
BpmI CTGGAG 1 cut(s) 686
Bpu10I CCTNAGC 1 cut(s) 113
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 2 cut(s) 12, 128
BsaWI WCCGGW 2 cut(s) 212, 501
BsaXI ACNNNNNCTCC 2 cut(s) 659, 689
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse1I ACTGG 2 cut(s) 181, 669
Bse21I CCTNAGG 2 cut(s) 441, 611
BseAI TCCGGA 2 cut(s) 212, 501
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 2 cut(s) 12, 128
BseGI GGATG 4 cut(s) 485, 518, 544, 601
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 8 cut(s) 30, 99, 126, 127, 279, 354, 455, 602
BseNI ACTGG 2 cut(s) 181, 669
BseSI GKGCMC 1 cut(s) 604
BseXI GCAGC 3 cut(s) 73, 470, 668
BseYI CCCAGC 2 cut(s) 118, 356
BshFI GGCC 2 cut(s) 361, 484
BsiHKAI GWGCWC 2 cut(s) 114, 604
BsiSI CCGG 2 cut(s) 213, 502
BslFI GGGAC 2 cut(s) 124, 397
BslI CCNNNNNNNGG 1 cut(s) 69
BsmFI GGGAC 2 cut(s) 124, 397
BsnI GGCC 2 cut(s) 361, 484
Bsp1286I GDGCHC 2 cut(s) 114, 604
Bsp13I TCCGGA 2 cut(s) 212, 501
Bsp143I GATC 4 cut(s) 284, 558, 622, 649
BspANI GGCC 2 cut(s) 361, 484
BspCNI CTCAG 8 cut(s) 31, 100, 126, 127, 280, 355, 454, 603
BspEI TCCGGA 2 cut(s) 212, 501
BspLI GGNNCC 1 cut(s) 217
BspMAI CTGCAG 2 cut(s) 391, 460
BspPI GGATC 2 cut(s) 292, 657
BsrI ACTGG 2 cut(s) 181, 669
BssECI CCNNGG 2 cut(s) 12, 128
BssMI GATC 4 cut(s) 284, 558, 622, 649
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 1 cut(s) 411
BstACI GRCGYC 1 cut(s) 237
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 8 cut(s) 39, 108, 113, 135, 288, 363, 441, 611
BstENI CCTNNNNNAGG 1 cut(s) 67
BstF5I GGATG 4 cut(s) 485, 518, 544, 601
BstKTI GATC 4 cut(s) 287, 561, 625, 652
BstMBI GATC 4 cut(s) 284, 558, 622, 649
BstMWI GCNNNNNNNGC 3 cut(s) 124, 458, 464
BstNI CCWGG 1 cut(s) 129
BstSCI CCNGG 1 cut(s) 127
BstSFI CTRYAG 2 cut(s) 387, 456
BstSLI GKGCMC 1 cut(s) 604
BstV1I GCAGC 3 cut(s) 73, 470, 668
BstX2I RGATCY 2 cut(s) 284, 649
BstYI RGATCY 2 cut(s) 284, 649
Bsu36I CCTNAGG 2 cut(s) 441, 611
BsuRI GGCC 2 cut(s) 361, 484
BtsCI GGATG 4 cut(s) 485, 518, 544, 601
BtsI GCAGTG 2 cut(s) 185, 396
BtsIMutI CAGTG 4 cut(s) 105, 185, 396, 662
Cac8I GCNNGC 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 359
CseI GACGC 1 cut(s) 245
Csp6I GTAC 1 cut(s) 404
CviAII CATG 3 cut(s) 20, 428, 592
CviQI GTAC 1 cut(s) 404
DdeI CTNAG 8 cut(s) 39, 108, 113, 135, 288, 363, 441, 611
DpnI GATC 4 cut(s) 286, 560, 624, 651
DpnII GATC 4 cut(s) 284, 558, 622, 649
EaeI YGGCCR 1 cut(s) 482
Ecl136II GAGCTC 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 114
Eco53kI GAGCTC 1 cut(s) 112
Eco81I CCTNAGG 2 cut(s) 441, 611
EcoICRI GAGCTC 1 cut(s) 112
EcoNI CCTNNNNNAGG 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 508
EcoRII CCWGG 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 3 cut(s) 23, 431, 595
FaiI YATR 9 cut(s) 8, 21, 29, 429, 579, 593, 607, 636, 645
FaqI GGGAC 2 cut(s) 124, 397
FatI CATG 3 cut(s) 19, 427, 591
Fnu4HI GCNGC 3 cut(s) 87, 459, 682
FokI GGATG 4 cut(s) 492, 505, 551, 608
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 3 cut(s) 87, 459, 682
FspBI CTAG 1 cut(s) 327
GluI GCNGC 3 cut(s) 87, 459, 682
GsaI CCCAGC 2 cut(s) 122, 360
GsuI CTGGAG 1 cut(s) 686
HaeIII GGCC 2 cut(s) 361, 484
HapII CCGG 2 cut(s) 213, 502
HgaI GACGC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 3 cut(s) 23, 431, 595
HincII GTYRAC 1 cut(s) 377
HindII GTYRAC 1 cut(s) 377
HinfI GANTC 3 cut(s) 41, 137, 699
HpaII CCGG 2 cut(s) 213, 502
Hpy166II GTNNAC 2 cut(s) 377, 602
Hpy188I TCNGA 6 cut(s) 40, 67, 136, 235, 289, 563
Hpy188III TCNNGA 2 cut(s) 213, 502
Hpy8I GTNNAC 2 cut(s) 377, 602
HpyAV CCTTC 2 cut(s) 60, 545
HpyCH4III ACNGT 1 cut(s) 411
HpyCH4V TGCA 5 cut(s) 389, 427, 458, 548, 602
HpyF10VI GCNNNNNNNGC 3 cut(s) 124, 458, 464
HpyF3I CTNAG 8 cut(s) 39, 108, 113, 135, 288, 363, 441, 611
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 3 cut(s) 23, 431, 595
Kpn2I TCCGGA 2 cut(s) 212, 501
Kzo9I GATC 4 cut(s) 284, 558, 622, 649
LmnI GCTCC 6 cut(s) 16, 83, 215, 248, 457, 563
Lsp1109I GCAGC 3 cut(s) 73, 470, 668
LweI GCATC 2 cut(s) 527, 535
MaeI CTAG 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 311
MalI GATC 4 cut(s) 286, 560, 624, 651
MboI GATC 4 cut(s) 284, 558, 622, 649
MboII GAAGA 3 cut(s) 445, 517, 682
MfeI CAATTG 1 cut(s) 690
MflI RGATCY 2 cut(s) 284, 649
MhlI GDGCHC 2 cut(s) 114, 604
MlsI TGGCCA 1 cut(s) 484
MluCI AATT 3 cut(s) 489, 508, 690
MluNI TGGCCA 1 cut(s) 484
MlyI GAGTC 2 cut(s) 50, 146
MmeI TCCRAC 1 cut(s) 457
Mox20I TGGCCA 1 cut(s) 484
MroI TCCGGA 2 cut(s) 212, 501
MroXI GAANNNNTTC 1 cut(s) 674
MscI TGGCCA 1 cut(s) 484
MseI TTAA 2 cut(s) 336, 709
Msp20I TGGCCA 1 cut(s) 484
MspA1I CMGCKG 1 cut(s) 681
MspI CCGG 2 cut(s) 213, 502
MspR9I CCNGG 1 cut(s) 129
MunI CAATTG 1 cut(s) 690
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 124, 458, 464
NdeII GATC 4 cut(s) 284, 558, 622, 649
NlaIII CATG 3 cut(s) 23, 431, 595
NlaIV GGNNCC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 674
PfeI GAWTC 1 cut(s) 699
PkrI GCNGC 3 cut(s) 88, 460, 683
PleI GAGTC 2 cut(s) 49, 145
PpsI GAGTC 2 cut(s) 49, 145
Psp124BI GAGCTC 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 127
PspFI CCCAGC 2 cut(s) 118, 356
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 1 cut(s) 359
PstI CTGCAG 2 cut(s) 391, 460
PsuI RGATCY 2 cut(s) 284, 649
PvuII CAGCTG 1 cut(s) 681
RsaI GTAC 1 cut(s) 405
RsaNI GTAC 1 cut(s) 404
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 2 cut(s) 336, 709
SatI GCNGC 3 cut(s) 87, 459, 682
Sau3AI GATC 4 cut(s) 284, 558, 622, 649
Sau96I GGNCC 1 cut(s) 359
SchI GAGTC 2 cut(s) 50, 146
ScrFI CCNGG 1 cut(s) 129
SduI GDGCHC 2 cut(s) 114, 604
SfaNI GCATC 2 cut(s) 527, 535
SfcI CTRYAG 2 cut(s) 387, 456
Sse9I AATT 3 cut(s) 489, 508, 690
SspMI CTAG 1 cut(s) 327
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 1 cut(s) 127
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 1 cut(s) 411
TaqI TCGA 1 cut(s) 621
TasI AATT 3 cut(s) 489, 508, 690
TfiI GAWTC 1 cut(s) 699
Tru1I TTAA 2 cut(s) 336, 709
Tru9I TTAA 2 cut(s) 336, 709
TscAI CASTG 4 cut(s) 112, 192, 396, 669
TseI GCWGC 3 cut(s) 86, 458, 681
TspDTI ATGAA 1 cut(s) 486
TspRI CASTG 4 cut(s) 112, 192, 396, 669
VneI GTGCAC 1 cut(s) 600
XagI CCTNNNNNAGG 1 cut(s) 67
XapI RAATTY 1 cut(s) 508
XmnI GAANNNNTTC 1 cut(s) 674
XspI CTAG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.