Rh2DG426100

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003380
Physical Location & Seq
Forward (+)
61742375 .. 61746475
4101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG426100.1

Sequence Viewer

Length: 843 bp
ATGTCTGTACCACTGGAACATGACTACATAGGCTTAGCAGAGACTTCTTCAATGGAAAGAAGCTCTGATAGGATTTCCTCTTCCTCCTCCTCTGCCCACTCAACTACAGAGGATGTCAAGACCTCTGCTTTCAACCTCAAGGAGACTGAGCTGAGGCTTGGCTTGCCTGGCTCTCACTCTCCTGAGAGAAAACCAGGAATTGCTCTTTCAATTTTTCGCAAAGATTTGGAGGACAATAAGCAAAATGGATATCCTCTAAACCCTTCAAAAACCAACCCTGTGTCTGGTGCTAAGAGGGGTTTCTCTGATGCCATTGATGGGTCTTCTGAGAACTGGGTTTTTCGTATGAGTAATGGATCTGAAGCTGATAAAGGAGCAGTCTTGTTCTCTCCTAAAGGTGAGAGTAATGCTAAAACACAACAGCTATGTGTGTCTGGACAAACCAAGAAAGAGGTTGCTGCTTCTGTTGTTCCTCAGTCACCAAAGCCAGTCCAAGAGAAGCAGAAGTCTCAGGTCTCCGAGCATGTTAGTCCTCCTGCTGCCAAGGCACAGGTGGTAGGATGGCCCCCAATTCGATCATTTCGAAAGAACACCATGGCCTCAAATTTGGCAAAGAACACTGATGATGCTGAGGGAAAACAAGGGTCTGGGTGTCTTTATGTGAAGGTTAGCATGGATGGTGCTCCATATCTAAGGAAGGTTGACCTTAAAATGTACAACAACTACATGGAACTCTCTATGGCTTTGGAGAAGATGTTCAGCTGCTTCACTATTGGTACTGTTCTCTTTCTCTTTCTCTTTCACTCTTATCAGTTGCAAGGCAGGCATGCACATGCTGACTGA

Protein Analysis

280

Amino Acids

30.59

Weight (kDa)

8.81

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 48 - 260 6.1e-49 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 364
AcsI RAATTY 1 cut(s) 604
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 3 cut(s) 9, 716, 778
AfiI CCNNNNNNNGG 2 cut(s) 284, 318
AgsI TTSAA 4 cut(s) 51, 133, 210, 267
AjnI CCWGG 2 cut(s) 166, 193
AloI GAACNNNNNNTCC 2 cut(s) 740, 772
AluBI AGCT 5 cut(s) 63, 151, 365, 424, 762
AluI AGCT 5 cut(s) 63, 151, 365, 424, 762
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 4 cut(s) 35, 137, 513, 520
AlwI GGATC 1 cut(s) 364
AoxI GGCC 2 cut(s) 563, 597
ApeKI GCWGC 3 cut(s) 458, 539, 762
ApoI RAATTY 1 cut(s) 604
Asp700I GAANNNNTTC 1 cut(s) 755
AspS9I GGNCC 1 cut(s) 564
AsuHPI GGTGA 2 cut(s) 410, 471
AsuII TTCGAA 1 cut(s) 583
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 685
BbvCI CCTCAGC 2 cut(s) 152, 630
BbvI GCAGC 3 cut(s) 445, 526, 749
BccI CCATC 3 cut(s) 311, 555, 671
BciT130I CCWGG 2 cut(s) 168, 195
BcoDI GTCTC 4 cut(s) 35, 137, 513, 520
BfmI CTRYAG 1 cut(s) 105
BisI GCNGC 3 cut(s) 459, 540, 763
BlpI GCTNAGC 1 cut(s) 34
BlsI GCNGC 3 cut(s) 460, 541, 764
Bme1390I CCNGG 2 cut(s) 168, 195
BmgT120I GGNCC 1 cut(s) 564
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 2 cut(s) 168, 195
BmrI ACTGGG 1 cut(s) 343
BmsI GCATC 2 cut(s) 298, 616
BmuI ACTGGG 1 cut(s) 343
BpiI GAAGAC 1 cut(s) 315
Bpu10I CCTNAGC 2 cut(s) 152, 630
Bpu1102I GCTNAGC 1 cut(s) 34
Bpu14I TTCGAA 1 cut(s) 583
BpuEI CTTGAG 1 cut(s) 122
BsaI GGTCTC 1 cut(s) 520
BsaJI CCNNGG 2 cut(s) 543, 594
BsaXI ACNNNNNCTCC 2 cut(s) 740, 770
Bsc4I CCNNNNNNNGG 2 cut(s) 284, 318
Bse1I ACTGG 3 cut(s) 18, 338, 488
BseBI CCWGG 2 cut(s) 168, 195
BseDI CCNNGG 2 cut(s) 543, 594
BseGI GGATG 3 cut(s) 118, 566, 682
BseLI CCNNNNNNNGG 2 cut(s) 284, 318
BseMII CTCAG 7 cut(s) 138, 143, 174, 318, 488, 524, 621
BseNI ACTGG 3 cut(s) 18, 338, 488
BseRI GAGGAG 2 cut(s) 76, 79
BseXI GCAGC 3 cut(s) 445, 526, 749
BshFI GGCC 2 cut(s) 565, 599
BsiHKAI GWGCWC 1 cut(s) 685
BslI CCNNNNNNNGG 2 cut(s) 284, 318
BsmAI GTCTC 4 cut(s) 35, 137, 513, 520
BsnI GGCC 2 cut(s) 565, 599
Bso31I GGTCTC 1 cut(s) 520
Bsp119I TTCGAA 1 cut(s) 583
Bsp1286I GDGCHC 1 cut(s) 685
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 2 cut(s) 356, 575
Bsp1720I GCTNAGC 1 cut(s) 34
Bsp19I CCATGG 1 cut(s) 594
BspANI GGCC 2 cut(s) 565, 599
BspCNI CTCAG 7 cut(s) 139, 144, 175, 319, 487, 523, 622
BspLI GGNNCC 1 cut(s) 566
BspPI GGATC 1 cut(s) 364
BspT104I TTCGAA 1 cut(s) 583
BspTNI GGTCTC 1 cut(s) 520
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 3 cut(s) 18, 338, 488
BssECI CCNNGG 2 cut(s) 543, 594
BssMI GATC 2 cut(s) 356, 575
BssT1I CCWWGG 2 cut(s) 543, 594
Bst2UI CCWGG 2 cut(s) 168, 195
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 1 cut(s) 85
BstAUI TGTACA 1 cut(s) 714
BstBI TTCGAA 1 cut(s) 583
BstC8I GCNNGC 3 cut(s) 164, 824, 828
BstDSI CCRYGG 1 cut(s) 594
BstF5I GGATG 3 cut(s) 118, 566, 682
BstKTI GATC 2 cut(s) 359, 578
BstMAI GTCTC 4 cut(s) 35, 137, 513, 520
BstMBI GATC 2 cut(s) 356, 575
BstMWI GCNNNNNNNGC 4 cut(s) 163, 168, 545, 823
BstNI CCWGG 2 cut(s) 168, 195
BstNSI RCATGY 3 cut(s) 527, 830, 836
BstSCI CCNGG 2 cut(s) 166, 193
BstSFI CTRYAG 1 cut(s) 105
BstV1I GCAGC 3 cut(s) 445, 526, 749
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 1 cut(s) 356
BstYI RGATCY 1 cut(s) 356
BsuRI GGCC 2 cut(s) 565, 599
BtgI CCRYGG 1 cut(s) 594
BtsCI GGATG 3 cut(s) 118, 566, 682
BtsIMutI CAGTG 2 cut(s) 11, 618
Cac8I GCNNGC 3 cut(s) 164, 824, 828
Cfr13I GGNCC 1 cut(s) 564
Csp6I GTAC 3 cut(s) 8, 715, 777
CviAII CATG 7 cut(s) 20, 524, 595, 673, 727, 827, 833
CviQI GTAC 3 cut(s) 8, 715, 777
DpnI GATC 2 cut(s) 358, 577
DpnII GATC 2 cut(s) 356, 575
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
Eco130I CCWWGG 2 cut(s) 543, 594
Eco31I GGTCTC 1 cut(s) 520
Eco32I GATATC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 381
EcoRII CCWGG 2 cut(s) 166, 193
EcoRV GATATC 1 cut(s) 251
EcoT14I CCWWGG 2 cut(s) 543, 594
ErhI CCWWGG 2 cut(s) 543, 594
FaeI CATG 7 cut(s) 23, 527, 598, 676, 730, 830, 836
FatI CATG 7 cut(s) 19, 523, 594, 672, 726, 826, 832
Fnu4HI GCNGC 3 cut(s) 459, 540, 763
FokI GGATG 3 cut(s) 125, 573, 689
Fsp4HI GCNGC 3 cut(s) 459, 540, 763
GluI GCNGC 3 cut(s) 459, 540, 763
HaeIII GGCC 2 cut(s) 565, 599
Hin1II CATG 7 cut(s) 23, 527, 598, 676, 730, 830, 836
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HphI GGTGA 2 cut(s) 410, 471
Hpy166II GTNNAC 1 cut(s) 703
Hpy188I TCNGA 5 cut(s) 67, 307, 328, 361, 520
Hpy188III TCNNGA 3 cut(s) 118, 182, 435
Hpy8I GTNNAC 1 cut(s) 703
HpyAV CCTTC 3 cut(s) 273, 658, 691
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4V TGCA 2 cut(s) 817, 830
HpyF10VI GCNNNNNNNGC 4 cut(s) 163, 168, 545, 823
Hsp92II CATG 7 cut(s) 23, 527, 598, 676, 730, 830, 836
Kzo9I GATC 2 cut(s) 356, 575
LmnI GCTCC 2 cut(s) 374, 688
Lsp1109I GCAGC 3 cut(s) 445, 526, 749
LweI GCATC 2 cut(s) 298, 616
MaeIII GTNAC 1 cut(s) 477
MalI GATC 2 cut(s) 358, 577
MboI GATC 2 cut(s) 356, 575
MboII GAAGA 4 cut(s) 39, 72, 315, 763
MflI RGATCY 1 cut(s) 356
MhlI GDGCHC 1 cut(s) 685
MluCI AATT 4 cut(s) 198, 210, 570, 604
MroXI GAANNNNTTC 1 cut(s) 755
MseI TTAA 1 cut(s) 708
MslI CAYNNNNRTG 1 cut(s) 831
MspA1I CMGCKG 1 cut(s) 762
MspR9I CCNGG 2 cut(s) 168, 195
MvaI CCWGG 2 cut(s) 168, 195
MwoI GCNNNNNNNGC 4 cut(s) 163, 168, 545, 823
NcoI CCATGG 1 cut(s) 594
NdeII GATC 2 cut(s) 356, 575
NlaIII CATG 7 cut(s) 23, 527, 598, 676, 730, 830, 836
NlaIV GGNNCC 1 cut(s) 566
NmuCI GTSAC 1 cut(s) 477
NspI RCATGY 3 cut(s) 527, 830, 836
NspV TTCGAA 1 cut(s) 583
PaeI GCATGC 1 cut(s) 830
PcsI WCGNNNNNNNCGW 1 cut(s) 580
PdmI GAANNNNTTC 1 cut(s) 755
PkrI GCNGC 3 cut(s) 460, 541, 764
Psp6I CCWGG 2 cut(s) 166, 193
PspGI CCWGG 2 cut(s) 166, 193
PspN4I GGNNCC 1 cut(s) 566
PspPI GGNCC 1 cut(s) 564
PsuI RGATCY 1 cut(s) 356
PvuII CAGCTG 1 cut(s) 762
RsaI GTAC 3 cut(s) 9, 716, 778
RsaNI GTAC 3 cut(s) 8, 715, 777
RseI CAYNNNNRTG 1 cut(s) 831
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 3 cut(s) 459, 540, 763
Sau3AI GATC 2 cut(s) 356, 575
Sau96I GGNCC 1 cut(s) 564
ScrFI CCNGG 2 cut(s) 168, 195
SduI GDGCHC 1 cut(s) 685
SfaNI GCATC 2 cut(s) 298, 616
SfcI CTRYAG 1 cut(s) 105
SfuI TTCGAA 1 cut(s) 583
SmiMI CAYNNNNRTG 1 cut(s) 831
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SphI GCATGC 1 cut(s) 830
Sse9I AATT 4 cut(s) 198, 210, 570, 604
StyD4I CCNGG 2 cut(s) 166, 193
StyI CCWWGG 2 cut(s) 543, 594
TaaI ACNGT 1 cut(s) 781
TaqI TCGA 2 cut(s) 574, 583
TasI AATT 4 cut(s) 198, 210, 570, 604
TatI WGTACW 1 cut(s) 714
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TscAI CASTG 2 cut(s) 18, 625
TseFI GTSAC 1 cut(s) 477
TseI GCWGC 3 cut(s) 458, 539, 762
Tsp45I GTSAC 1 cut(s) 477
TspRI CASTG 2 cut(s) 18, 625
XapI RAATTY 1 cut(s) 604
XceI RCATGY 3 cut(s) 527, 830, 836
XcmI CCANNNNNNNNNTGG 1 cut(s) 550
XmnI GAANNNNTTC 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.