RchiOBHm_Chr2g0139301

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003380
Physical Location & Seq
Forward (+)
56917205 .. 56920083
2879 bp
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UTR
Exon/CDS
Intron
PRQ50987

Sequence Viewer

Length: 1017 bp
ATGTCTGTACCACTGGAACATGACTACATAGGGTTAGCAGAGACTTCTTCAATGGAAAGAAGCTCTGATAGGATTTCCTCTTCCTCCTCCTCTGCCCACTCAACTACAGAGGATGTCAAGACCTCTGCTTTCAACCTCAAGGAGACTGAGCTGAGGCTTGGCTTGCCTGGCTCTCACTCTCCTGAGAGAAAACCAGGAATTGCTCTTTCAATTTTTCGCAAAGATTTGGAGGACAATAAGCAAAATGGATATCCTCTAAACCCTTCAAAAACCAACCCTGTGTCTGGTGCTAAGAGGGGTTTCTCTGATGCCATTGATGGGTCTTCTGAGAAATGGGTTTTTCGTATGAGTAATGGATCTGAAGCTGATAAAGGAGCAGTCTTGTTCTCTCCTAAAGGTGAGAGTAATGCTAAAACACAACAGCTATGTGTGTCTGGACAAACCAAGAAAGAGGTTGCTGCTTCTGTTGTTCCTCAGTCACCAAAGCCAGTCCAAGAGAAGCAGAAGTCTCAGGTCTCTGAGCATGTTAGTCCTCCTGCTGCCAAGGCACAGGTGGTAGGATGGCCCCCAATTCGATCATTTCGAAAGAACACCATGGCCTCAAATTTGGCAAAGAACACTGATGATGCTGAGGGAAAACAAGGGTCTGGGTGTCTTTATGTGAAGGTTAGCATGGATGGTGCTCCATATCTAAGGAAGGTTGACCTTAAAATGTACAACAACTACATGGAACTCTCTATGGCTTTGGAGAAGATGTTCAGCTGCTTCACCATTGGGCAGTGCAGTTCTAATGGATTTCCAGAGAAGGATGGCCTGAGTGAGAGTCGGTTGATGGATCTTCTCCATGGTTCTGAATACGTTCTTACTTTTGAAGACAAGGACGATGACTGGATGCTTGTTGGTGATGTTCCTTGGGGGATGTTCACTGAGACGTGTAGGAAACTAAGGATCATGAAAGGTTCAGAAGCAATTGGACTTGCTCCAAGGGCTATGGAGAAGTGCAAGAACCGCAACTAG

Protein Analysis

338

Amino Acids

37.08

Weight (kDa)

8.09

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 48 - 329 1.5e-90 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 1009
AclWI GGATC 3 cut(s) 364, 843, 956
AcsI RAATTY 1 cut(s) 604
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 2 cut(s) 9, 716
AfiI CCNNNNNNNGG 2 cut(s) 284, 318
AflIII ACRYGT 1 cut(s) 932
AgsI TTSAA 5 cut(s) 51, 133, 210, 267, 872
AjiI CACGTC 1 cut(s) 933
AjnI CCWGG 2 cut(s) 166, 193
AloI GAACNNNNNNTCC 2 cut(s) 740, 772
AluBI AGCT 5 cut(s) 63, 151, 365, 424, 762
AluI AGCT 5 cut(s) 63, 151, 365, 424, 762
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 5 cut(s) 35, 137, 513, 520, 923
AlwI GGATC 3 cut(s) 364, 843, 956
AoxI GGCC 3 cut(s) 563, 597, 811
ApeKI GCWGC 3 cut(s) 458, 539, 762
ApoI RAATTY 1 cut(s) 604
Asp700I GAANNNNTTC 2 cut(s) 755, 858
AspS9I GGNCC 1 cut(s) 564
AsuHPI GGTGA 4 cut(s) 410, 471, 760, 914
AsuII TTCGAA 1 cut(s) 583
BbsI GAAGAC 2 cut(s) 315, 879
Bbv12I GWGCWC 1 cut(s) 685
BbvCI CCTCAGC 2 cut(s) 152, 630
BbvI GCAGC 3 cut(s) 445, 526, 749
BccI CCATC 5 cut(s) 311, 555, 671, 803, 826
BciT130I CCWGG 2 cut(s) 168, 195
BcoDI GTCTC 5 cut(s) 35, 137, 513, 520, 923
BfaI CTAG 1 cut(s) 1015
BfmI CTRYAG 1 cut(s) 105
BisI GCNGC 3 cut(s) 459, 540, 763
BlsI GCNGC 3 cut(s) 460, 541, 764
Bme1390I CCNGG 2 cut(s) 168, 195
BmgBI CACGTC 1 cut(s) 933
BmgT120I GGNCC 1 cut(s) 564
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 2 cut(s) 168, 195
BmsI GCATC 3 cut(s) 298, 616, 882
BpiI GAAGAC 2 cut(s) 315, 879
Bpu10I CCTNAGC 2 cut(s) 152, 630
Bpu14I TTCGAA 1 cut(s) 583
BpuEI CTTGAG 1 cut(s) 122
BsaI GGTCTC 1 cut(s) 520
BsaJI CCNNGG 5 cut(s) 543, 594, 844, 911, 983
BsaXI ACNNNNNCTCC 2 cut(s) 740, 770
Bsc4I CCNNNNNNNGG 2 cut(s) 284, 318
Bse1I ACTGG 3 cut(s) 18, 488, 893
BseBI CCWGG 2 cut(s) 168, 195
BseDI CCNNGG 5 cut(s) 543, 594, 844, 911, 983
BseGI GGATG 6 cut(s) 118, 566, 682, 814, 897, 924
BseLI CCNNNNNNNGG 2 cut(s) 284, 318
BseNI ACTGG 3 cut(s) 18, 488, 893
BseRI GAGGAG 2 cut(s) 76, 79
BseXI GCAGC 3 cut(s) 445, 526, 749
BsgI GTGCAG 1 cut(s) 802
BshFI GGCC 3 cut(s) 565, 599, 813
BsiHKAI GWGCWC 1 cut(s) 685
BslI CCNNNNNNNGG 2 cut(s) 284, 318
BsmAI GTCTC 5 cut(s) 35, 137, 513, 520, 923
BsmBI CGTCTC 1 cut(s) 923
BsnI GGCC 3 cut(s) 565, 599, 813
Bso31I GGTCTC 1 cut(s) 520
Bsp119I TTCGAA 1 cut(s) 583
Bsp1286I GDGCHC 1 cut(s) 685
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 4 cut(s) 356, 575, 835, 948
Bsp19I CCATGG 2 cut(s) 594, 844
BspACI CCGC 1 cut(s) 1009
BspANI GGCC 3 cut(s) 565, 599, 813
BspHI TCATGA 1 cut(s) 951
BspLI GGNNCC 1 cut(s) 566
BspPI GGATC 3 cut(s) 364, 843, 956
BspT104I TTCGAA 1 cut(s) 583
BspTNI GGTCTC 1 cut(s) 520
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 3 cut(s) 18, 488, 893
BssECI CCNNGG 5 cut(s) 543, 594, 844, 911, 983
BssMI GATC 4 cut(s) 356, 575, 835, 948
BssT1I CCWWGG 5 cut(s) 543, 594, 844, 911, 983
Bst2UI CCWGG 2 cut(s) 168, 195
Bst6I CTCTTC 1 cut(s) 85
BstAUI TGTACA 1 cut(s) 714
BstBI TTCGAA 1 cut(s) 583
BstC8I GCNNGC 1 cut(s) 164
BstDSI CCRYGG 2 cut(s) 594, 844
BstF5I GGATG 6 cut(s) 118, 566, 682, 814, 897, 924
BstKTI GATC 4 cut(s) 359, 578, 838, 951
BstMAI GTCTC 5 cut(s) 35, 137, 513, 520, 923
BstMBI GATC 4 cut(s) 356, 575, 835, 948
BstMWI GCNNNNNNNGC 5 cut(s) 163, 168, 545, 986, 1008
BstNI CCWGG 2 cut(s) 168, 195
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 2 cut(s) 166, 193
BstSFI CTRYAG 1 cut(s) 105
BstV1I GCAGC 3 cut(s) 445, 526, 749
BstV2I GAAGAC 2 cut(s) 315, 879
BstX2I RGATCY 2 cut(s) 356, 835
BstYI RGATCY 2 cut(s) 356, 835
BsuRI GGCC 3 cut(s) 565, 599, 813
BtgI CCRYGG 2 cut(s) 594, 844
BtrI CACGTC 1 cut(s) 933
BtsCI GGATG 6 cut(s) 118, 566, 682, 814, 897, 924
BtsI GCAGTG 1 cut(s) 785
BtsIMutI CAGTG 4 cut(s) 11, 618, 785, 924
Cac8I GCNNGC 1 cut(s) 164
CciI TCATGA 1 cut(s) 951
Cfr13I GGNCC 1 cut(s) 564
Csp6I GTAC 2 cut(s) 8, 715
CviAII CATG 7 cut(s) 20, 524, 595, 673, 727, 845, 952
CviQI GTAC 2 cut(s) 8, 715
DpnI GATC 4 cut(s) 358, 577, 837, 950
DpnII GATC 4 cut(s) 356, 575, 835, 948
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
Eco130I CCWWGG 5 cut(s) 543, 594, 844, 911, 983
Eco31I GGTCTC 1 cut(s) 520
Eco32I GATATC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 381
EcoRII CCWGG 2 cut(s) 166, 193
EcoRV GATATC 1 cut(s) 251
EcoT14I CCWWGG 5 cut(s) 543, 594, 844, 911, 983
ErhI CCWWGG 5 cut(s) 543, 594, 844, 911, 983
Esp3I CGTCTC 1 cut(s) 923
FaeI CATG 7 cut(s) 23, 527, 598, 676, 730, 848, 955
FatI CATG 7 cut(s) 19, 523, 594, 672, 726, 844, 951
Fnu4HI GCNGC 3 cut(s) 459, 540, 763
FokI GGATG 6 cut(s) 125, 573, 689, 821, 904, 931
Fsp4HI GCNGC 3 cut(s) 459, 540, 763
FspBI CTAG 1 cut(s) 1015
GluI GCNGC 3 cut(s) 459, 540, 763
HaeIII GGCC 3 cut(s) 565, 599, 813
Hin1II CATG 7 cut(s) 23, 527, 598, 676, 730, 848, 955
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HinfI GANTC 1 cut(s) 823
HphI GGTGA 4 cut(s) 410, 471, 760, 914
Hpy166II GTNNAC 2 cut(s) 703, 924
Hpy188I TCNGA 7 cut(s) 67, 307, 328, 361, 520, 853, 964
Hpy188III TCNNGA 5 cut(s) 118, 182, 435, 800, 952
Hpy8I GTNNAC 2 cut(s) 703, 924
HpyAV CCTTC 4 cut(s) 273, 658, 691, 799
HpyCH4IV ACGT 2 cut(s) 858, 932
HpyCH4V TGCA 2 cut(s) 783, 1002
HpyF10VI GCNNNNNNNGC 5 cut(s) 163, 168, 545, 986, 1008
HpySE526I ACGT 2 cut(s) 858, 932
Hsp92II CATG 7 cut(s) 23, 527, 598, 676, 730, 848, 955
Kzo9I GATC 4 cut(s) 356, 575, 835, 948
LmnI GCTCC 3 cut(s) 374, 688, 985
Lsp1109I GCAGC 3 cut(s) 445, 526, 749
LweI GCATC 3 cut(s) 298, 616, 882
MaeI CTAG 1 cut(s) 1015
MaeII ACGT 2 cut(s) 858, 932
MaeIII GTNAC 1 cut(s) 477
MalI GATC 4 cut(s) 358, 577, 837, 950
MboI GATC 4 cut(s) 356, 575, 835, 948
MboII GAAGA 6 cut(s) 39, 72, 315, 763, 830, 884
MfeI CAATTG 1 cut(s) 969
MflI RGATCY 2 cut(s) 356, 835
MhlI GDGCHC 1 cut(s) 685
MluCI AATT 5 cut(s) 198, 210, 570, 604, 969
MlyI GAGTC 1 cut(s) 832
MroXI GAANNNNTTC 2 cut(s) 755, 858
MseI TTAA 1 cut(s) 708
MspA1I CMGCKG 1 cut(s) 762
MspR9I CCNGG 2 cut(s) 168, 195
MunI CAATTG 1 cut(s) 969
MvaI CCWGG 2 cut(s) 168, 195
MwoI GCNNNNNNNGC 5 cut(s) 163, 168, 545, 986, 1008
NcoI CCATGG 2 cut(s) 594, 844
NdeII GATC 4 cut(s) 356, 575, 835, 948
NlaIII CATG 7 cut(s) 23, 527, 598, 676, 730, 848, 955
NlaIV GGNNCC 1 cut(s) 566
NmuCI GTSAC 1 cut(s) 477
NspI RCATGY 1 cut(s) 527
NspV TTCGAA 1 cut(s) 583
PagI TCATGA 1 cut(s) 951
PcsI WCGNNNNNNNCGW 1 cut(s) 580
PdmI GAANNNNTTC 2 cut(s) 755, 858
PkrI GCNGC 3 cut(s) 460, 541, 764
PleI GAGTC 1 cut(s) 831
PpsI GAGTC 1 cut(s) 831
Psp6I CCWGG 2 cut(s) 166, 193
PspGI CCWGG 2 cut(s) 166, 193
PspN4I GGNNCC 1 cut(s) 566
PspPI GGNCC 1 cut(s) 564
PsuI RGATCY 2 cut(s) 356, 835
PvuII CAGCTG 1 cut(s) 762
RsaI GTAC 2 cut(s) 9, 716
RsaNI GTAC 2 cut(s) 8, 715
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 3 cut(s) 459, 540, 763
Sau3AI GATC 4 cut(s) 356, 575, 835, 948
Sau96I GGNCC 1 cut(s) 564
SchI GAGTC 1 cut(s) 832
ScrFI CCNGG 2 cut(s) 168, 195
SduI GDGCHC 1 cut(s) 685
SfaNI GCATC 3 cut(s) 298, 616, 882
SfcI CTRYAG 1 cut(s) 105
SfuI TTCGAA 1 cut(s) 583
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 5 cut(s) 198, 210, 570, 604, 969
SsiI CCGC 1 cut(s) 1009
SspMI CTAG 1 cut(s) 1015
StyD4I CCNGG 2 cut(s) 166, 193
StyI CCWWGG 5 cut(s) 543, 594, 844, 911, 983
TaiI ACGT 2 cut(s) 861, 935
TaqI TCGA 2 cut(s) 574, 583
TasI AATT 5 cut(s) 198, 210, 570, 604, 969
TatI WGTACW 1 cut(s) 714
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TscAI CASTG 4 cut(s) 18, 625, 785, 931
TseFI GTSAC 1 cut(s) 477
TseI GCWGC 3 cut(s) 458, 539, 762
Tsp45I GTSAC 1 cut(s) 477
TspDTI ATGAA 1 cut(s) 968
TspRI CASTG 4 cut(s) 18, 625, 785, 931
XapI RAATTY 1 cut(s) 604
XceI RCATGY 1 cut(s) 527
XcmI CCANNNNNNNNNTGG 1 cut(s) 550
XmnI GAANNNNTTC 2 cut(s) 755, 858
XspI CTAG 1 cut(s) 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.