pycom09g12420

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003380
Physical Location & Seq
Reverse (-)
10928610 .. 10931437
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g12420.1

Sequence Viewer

Length: 1011 bp
ATGTCTAGGCCACTGGAGCATGATTACATAGGCCTAACAGAGACTTCATCTTCTCCAATGGAAAGAAGCTCTGAGAAGATTTCATCTTCCACTTCCTCTGCCCTCTCGTCCACCAGAGCAGAGGAAGAGAAGACCTCTTCCTCCGCCATCAACCTCAAGGAGACTGAGCTCAGGCTTGGCCTGCCTGGGTCCCACTCTCCTGACAGAAAAAAACCATCAGGGCTTGGACTTGGGGTCTCATTTTTTGGGAAAGATTTGGAGGAGAATAACAAGCCAACTGCGTTTTCTCCAAACCCTCCAAAGAACCCTGTGTCAGGGGCAAAAAGGGGATTCTCTGACGCCATTGATGGGTGTTCTGAGAAATGGGTTTTTACCATGAGTAATGGATCTGAGGTTGATGTGGGTAAAGCAGGAGTCTTGGGTTCTCCATTAGCCAAGAAGGAGGTTTCTTCCGTTCCTCTGTCGCCAAAGCCAGCAGCTCAGTTGGAGAAGAAGAACACTCTGGCCTCTGAGCATGCTGCTGCTTCTCCTTCTTGCAAGGCACAGGTGGTAGGATGGCCCCCAATTCGATCATTTCGGAAGAATTCCATGGCCTCTAGTCTGGCGAAAAACAACGATGACGCTGCGGGCAAACAAGGGTCCGGGTGCCTTTATGTGAAGGTTAGCATGGACGGCGCTCCATACCTGCGAAAAGTTGACCTTAAAACCTACAACAACTACACTGAACTTTCCATGGCTCTGGAGAAGATGTTTAGCTGCTTCACCATTGGGCAGTGCAGTTCTAATGGACTTCCAAAGCGAGATGGTCTGAGTGTGAGTCGATTGATGGATCTTCTCAATGGTTCTGAATTTGTTCTGACTTTCGAGGACAAGGATGATGACTGGATGCTTGTTGGCGATGTTCCTTGGCAGATGTTCACTGAGACCTGTAGGAGGCTGAGGATCATGAAGGGTTCTGAAGCAATCGGACTAGCTCCAAGGGCCGCGGGGAAGTGCAAGAACCGCAATTAA

Protein Analysis

337

Amino Acids

36.2

Weight (kDa)

8.83

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 54 - 327 2.5e-87 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 693
AccB1I GGYRCC 1 cut(s) 645
AccII CGCG 1 cut(s) 986
AciI CCGC 5 cut(s) 144, 626, 984, 986, 1003
AclWI GGATC 3 cut(s) 394, 837, 950
AcsI RAATTY 2 cut(s) 583, 848
AcuI CTGAAG 1 cut(s) 978
AcyI GRCGYC 1 cut(s) 339
AfiI CCNNNNNNNGG 3 cut(s) 314, 348, 933
AhdI GACNNNNNGTC 1 cut(s) 233
AjnI CCWGG 1 cut(s) 184
AjuI GAANNNNNNNTTGG 2 cut(s) 268, 300
AluBI AGCT 5 cut(s) 69, 169, 479, 756, 974
AluI AGCT 5 cut(s) 69, 169, 479, 756, 974
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 4 cut(s) 35, 155, 241, 917
AlwI GGATC 3 cut(s) 394, 837, 950
AoxI GGCC 7 cut(s) 8, 31, 178, 504, 557, 591, 981
ApeKI GCWGC 5 cut(s) 476, 518, 521, 623, 756
ApoI RAATTY 2 cut(s) 583, 848
Asp700I GAANNNNTTC 1 cut(s) 852
AspLEI GCGC 1 cut(s) 677
AspS9I GGNCC 4 cut(s) 189, 558, 639, 981
AsuC2I CCSGG 1 cut(s) 643
AsuHPI GGTGA 1 cut(s) 754
AvaII GGWCC 2 cut(s) 189, 639
BanI GGYRCC 1 cut(s) 645
BanII GRGCYC 1 cut(s) 171
BbsI GAAGAC 1 cut(s) 137
Bbv12I GWGCWC 1 cut(s) 171
BbvCI CCTCAGC 1 cut(s) 938
BbvI GCAGC 5 cut(s) 488, 505, 508, 610, 743
BccI CCATC 6 cut(s) 155, 223, 341, 549, 797, 820
BceAI ACGGC 1 cut(s) 688
BcgI CGANNNNNNTGC 2 cut(s) 605, 639
BciT130I CCWGG 1 cut(s) 186
BcnI CCSGG 1 cut(s) 643
BcoDI GTCTC 4 cut(s) 35, 155, 241, 917
BfaI CTAG 3 cut(s) 6, 597, 971
BfmI CTRYAG 1 cut(s) 928
BfoI RGCGCY 1 cut(s) 678
BfuAI ACCTGC 1 cut(s) 693
BisI GCNGC 6 cut(s) 477, 519, 522, 624, 757, 984
BlsI GCNGC 6 cut(s) 478, 520, 523, 625, 758, 985
Bme1390I CCNGG 2 cut(s) 186, 643
Bme18I GGWCC 2 cut(s) 189, 639
BmeRI GACNNNNNGTC 1 cut(s) 233
BmgT120I GGNCC 4 cut(s) 189, 558, 639, 981
BmiI GGNNCC 5 cut(s) 190, 191, 560, 640, 647
BmrFI CCNGG 2 cut(s) 186, 643
BmsI GCATC 1 cut(s) 876
BpiI GAAGAC 1 cut(s) 137
BplI GAGNNNNNCTC 2 cut(s) 119, 151
BpmI CTGGAG 2 cut(s) 35, 761
Bpu10I CCTNAGC 2 cut(s) 170, 938
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 643
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 2 cut(s) 241, 917
BsaJI CCNNGG 6 cut(s) 185, 588, 732, 905, 977, 984
BsaXI ACNNNNNCTCC 2 cut(s) 734, 764
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 348, 933
Bse1I ACTGG 2 cut(s) 18, 887
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 6 cut(s) 185, 588, 732, 905, 977, 984
BseGI GGATG 3 cut(s) 560, 880, 891
BseLI CCNNNNNNNGG 3 cut(s) 314, 348, 933
BseNI ACTGG 2 cut(s) 18, 887
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 5 cut(s) 488, 505, 508, 610, 743
BsgI GTGCAG 1 cut(s) 796
Bsh1236I CGCG 1 cut(s) 986
BshFI GGCC 7 cut(s) 10, 33, 180, 506, 559, 593, 983
BshNI GGYRCC 1 cut(s) 645
BsiHKAI GWGCWC 1 cut(s) 171
BsiSI CCGG 1 cut(s) 642
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 3 cut(s) 314, 348, 933
BsmAI GTCTC 4 cut(s) 35, 155, 241, 917
BsmFI GGGAC 1 cut(s) 175
BsnI GGCC 7 cut(s) 10, 33, 180, 506, 559, 593, 983
Bso31I GGTCTC 2 cut(s) 241, 917
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 4 cut(s) 386, 569, 829, 942
Bsp19I CCATGG 2 cut(s) 588, 732
BspACI CCGC 5 cut(s) 144, 626, 984, 986, 1003
BspANI GGCC 7 cut(s) 10, 33, 180, 506, 559, 593, 983
BspFNI CGCG 1 cut(s) 986
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 5 cut(s) 190, 191, 560, 640, 647
BspMI ACCTGC 1 cut(s) 693
BspPI GGATC 3 cut(s) 394, 837, 950
BspT107I GGYRCC 1 cut(s) 645
BspTNI GGTCTC 2 cut(s) 241, 917
BsrI ACTGG 2 cut(s) 18, 887
BssECI CCNNGG 6 cut(s) 185, 588, 732, 905, 977, 984
BssMI GATC 4 cut(s) 386, 569, 829, 942
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 4 cut(s) 588, 732, 905, 977
Bst2UI CCWGG 1 cut(s) 186
Bst6I CTCTTC 2 cut(s) 120, 142
BstACI GRCGYC 1 cut(s) 339
BstC8I GCNNGC 4 cut(s) 182, 474, 516, 628
BstDSI CCRYGG 3 cut(s) 588, 732, 984
BstENI CCTNNNNNAGG 2 cut(s) 312, 931
BstF5I GGATG 3 cut(s) 560, 880, 891
BstFNI CGCG 1 cut(s) 986
BstH2I RGCGCY 1 cut(s) 678
BstHHI GCGC 1 cut(s) 677
BstKTI GATC 4 cut(s) 389, 572, 832, 945
BstMAI GTCTC 4 cut(s) 35, 155, 241, 917
BstMBI GATC 4 cut(s) 386, 569, 829, 942
BstMWI GCNNNNNNNGC 5 cut(s) 16, 181, 672, 980, 1002
BstNI CCWGG 1 cut(s) 186
BstNSI RCATGY 1 cut(s) 518
BstSCI CCNGG 2 cut(s) 184, 641
BstSFI CTRYAG 1 cut(s) 928
BstUI CGCG 1 cut(s) 986
BstV1I GCAGC 5 cut(s) 488, 505, 508, 610, 743
BstV2I GAAGAC 1 cut(s) 137
BstX2I RGATCY 2 cut(s) 386, 829
BstXI CCANNNNNNTGG 1 cut(s) 739
BstYI RGATCY 2 cut(s) 386, 829
BsuRI GGCC 7 cut(s) 10, 33, 180, 506, 559, 593, 983
BtgI CCRYGG 3 cut(s) 588, 732, 984
BtgZI GCGATG 1 cut(s) 912
BtsCI GGATG 3 cut(s) 560, 880, 891
BtsI GCAGTG 1 cut(s) 779
BtsIMutI CAGTG 4 cut(s) 11, 720, 779, 918
BveI ACCTGC 1 cut(s) 693
Cac8I GCNNGC 4 cut(s) 182, 474, 516, 628
CciI TCATGA 1 cut(s) 945
CfoI GCGC 1 cut(s) 677
Cfr13I GGNCC 4 cut(s) 189, 558, 639, 981
Cfr42I CCGCGG 1 cut(s) 987
CseI GACGC 2 cut(s) 347, 629
CviAII CATG 7 cut(s) 20, 376, 515, 589, 667, 733, 946
DpnI GATC 4 cut(s) 388, 571, 831, 944
DpnII GATC 4 cut(s) 386, 569, 829, 942
DriI GACNNNNNGTC 1 cut(s) 233
Eam1104I CTCTTC 2 cut(s) 120, 142
Eam1105I GACNNNNNGTC 1 cut(s) 233
EarI CTCTTC 2 cut(s) 120, 142
EciI GGCGGA 1 cut(s) 133
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 4 cut(s) 588, 732, 905, 977
Eco147I AGGCCT 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 171
Eco31I GGTCTC 2 cut(s) 241, 917
Eco47I GGWCC 2 cut(s) 189, 639
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 978
EcoICRI GAGCTC 1 cut(s) 169
EcoNI CCTNNNNNAGG 2 cut(s) 312, 931
EcoO109I RGGNCCY 1 cut(s) 189
EcoRI GAATTC 1 cut(s) 583
EcoRII CCWGG 1 cut(s) 184
EcoT14I CCWWGG 4 cut(s) 588, 732, 905, 977
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 4 cut(s) 588, 732, 905, 977
FaeI CATG 7 cut(s) 23, 379, 518, 592, 670, 736, 949
FalI AAGNNNNNCTT 2 cut(s) 684, 716
FaqI GGGAC 1 cut(s) 175
FatI CATG 7 cut(s) 19, 375, 514, 588, 666, 732, 945
FauI CCCGC 2 cut(s) 619, 979
Fnu4HI GCNGC 6 cut(s) 477, 519, 522, 624, 757, 984
FokI GGATG 3 cut(s) 567, 887, 898
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 6 cut(s) 477, 519, 522, 624, 757, 984
FspBI CTAG 3 cut(s) 6, 597, 971
GlaI GCGC 1 cut(s) 676
GluI GCNGC 6 cut(s) 477, 519, 522, 624, 757, 984
GsuI CTGGAG 2 cut(s) 35, 761
HaeII RGCGCY 1 cut(s) 678
HaeIII GGCC 7 cut(s) 10, 33, 180, 506, 559, 593, 983
HapII CCGG 1 cut(s) 642
HgaI GACGC 2 cut(s) 347, 629
HhaI GCGC 1 cut(s) 677
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 7 cut(s) 23, 379, 518, 592, 670, 736, 949
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HincII GTYRAC 1 cut(s) 697
HindII GTYRAC 1 cut(s) 697
HinfI GANTC 3 cut(s) 330, 414, 817
HpaII CCGG 1 cut(s) 642
HphI GGTGA 1 cut(s) 754
Hpy166II GTNNAC 3 cut(s) 111, 697, 918
Hpy188III TCNNGA 3 cut(s) 200, 740, 946
Hpy8I GTNNAC 3 cut(s) 111, 697, 918
HpyAV CCTTC 4 cut(s) 433, 540, 652, 943
HpyCH4V TGCA 3 cut(s) 537, 777, 996
HpyF10VI GCNNNNNNNGC 5 cut(s) 16, 181, 672, 980, 1002
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 7 cut(s) 23, 379, 518, 592, 670, 736, 949
HspAI GCGC 1 cut(s) 675
KflI GGGWCCC 1 cut(s) 189
KspI CCGCGG 1 cut(s) 987
Kzo9I GATC 4 cut(s) 386, 569, 829, 942
LmnI GCTCC 3 cut(s) 16, 682, 979
Lsp1109I GCAGC 5 cut(s) 488, 505, 508, 610, 743
LweI GCATC 1 cut(s) 876
MaeI CTAG 3 cut(s) 6, 597, 971
MalI GATC 4 cut(s) 388, 571, 831, 944
MboI GATC 4 cut(s) 386, 569, 829, 942
MflI RGATCY 2 cut(s) 386, 829
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 4 cut(s) 564, 583, 848, 1006
MlyI GAGTC 2 cut(s) 423, 826
MmeI TCCRAC 1 cut(s) 465
MroXI GAANNNNTTC 1 cut(s) 852
MseI TTAA 2 cut(s) 702, 1009
MspA1I CMGCKG 1 cut(s) 986
MspI CCGG 1 cut(s) 642
MspR9I CCNGG 2 cut(s) 186, 643
MvaI CCWGG 1 cut(s) 186
MvnI CGCG 1 cut(s) 986
MwoI GCNNNNNNNGC 5 cut(s) 16, 181, 672, 980, 1002
NciI CCSGG 1 cut(s) 643
NcoI CCATGG 2 cut(s) 588, 732
NdeII GATC 4 cut(s) 386, 569, 829, 942
NlaIII CATG 7 cut(s) 23, 379, 518, 592, 670, 736, 949
NlaIV GGNNCC 5 cut(s) 190, 191, 560, 640, 647
NspI RCATGY 1 cut(s) 518
PaeI GCATGC 1 cut(s) 518
PagI TCATGA 1 cut(s) 945
PceI AGGCCT 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 852
PfeI GAWTC 1 cut(s) 330
PkrI GCNGC 6 cut(s) 478, 520, 523, 625, 758, 985
PleI GAGTC 2 cut(s) 422, 825
PpsI GAGTC 2 cut(s) 422, 825
PpuMI RGGWCCY 1 cut(s) 189
Psp124BI GAGCTC 1 cut(s) 171
Psp5II RGGWCCY 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 5 cut(s) 190, 191, 560, 640, 647
PspPI GGNCC 4 cut(s) 189, 558, 639, 981
PspPPI RGGWCCY 1 cut(s) 189
PsuI RGATCY 2 cut(s) 386, 829
SacI GAGCTC 1 cut(s) 171
SacII CCGCGG 1 cut(s) 987
SaqAI TTAA 2 cut(s) 702, 1009
SatI GCNGC 6 cut(s) 477, 519, 522, 624, 757, 984
Sau3AI GATC 4 cut(s) 386, 569, 829, 942
Sau96I GGNCC 4 cut(s) 189, 558, 639, 981
SchI GAGTC 2 cut(s) 423, 826
ScrFI CCNGG 2 cut(s) 186, 643
SduI GDGCHC 1 cut(s) 171
SfaNI GCATC 1 cut(s) 876
SfcI CTRYAG 1 cut(s) 928
Sfr303I CCGCGG 1 cut(s) 987
SgrBI CCGCGG 1 cut(s) 987
SinI GGWCC 2 cut(s) 189, 639
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
SphI GCATGC 1 cut(s) 518
Sse9I AATT 4 cut(s) 564, 583, 848, 1006
SseBI AGGCCT 1 cut(s) 33
SsiI CCGC 5 cut(s) 144, 626, 984, 986, 1003
SspMI CTAG 3 cut(s) 6, 597, 971
SstI GAGCTC 1 cut(s) 171
StuI AGGCCT 1 cut(s) 33
StyD4I CCNGG 2 cut(s) 184, 641
StyI CCWWGG 4 cut(s) 588, 732, 905, 977
TaqI TCGA 3 cut(s) 568, 820, 864
TasI AATT 4 cut(s) 564, 583, 848, 1006
TauI GCSGC 1 cut(s) 986
TfiI GAWTC 1 cut(s) 330
Tru1I TTAA 2 cut(s) 702, 1009
Tru9I TTAA 2 cut(s) 702, 1009
TscAI CASTG 4 cut(s) 18, 727, 779, 925
TseI GCWGC 5 cut(s) 476, 518, 521, 623, 756
TspDTI ATGAA 3 cut(s) 36, 72, 962
TspGWI ACGGA 1 cut(s) 442
TspRI CASTG 4 cut(s) 18, 727, 779, 925
VpaK11BI GGWCC 2 cut(s) 189, 639
XagI CCTNNNNNAGG 2 cut(s) 312, 931
XapI RAATTY 2 cut(s) 583, 848
XceI RCATGY 1 cut(s) 518
XmnI GAANNNNTTC 1 cut(s) 852
XspI CTAG 3 cut(s) 6, 597, 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.