Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002324

Physical Location & Seq

Genomic Coordinates Reverse (-)

32392797 .. 32397927

Gene Structure 5131 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1227200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2007 bp

ATGAATAGTTCAGACTATGACGTTTGCAATATTTCGTGGGCCCATTCAAGCCCAAGTAGTCCGAATGCCGAAGCTTTCCATAAAGCCCAAAGGCAATGTGAGAGAGCAAACCTTGTGTGCACATTAGACAAATGGCGTACGTCTTTACTAACTTACGCCCACTACAGTATTCGCCGGTCGTATTACTGGAAGAAATTGGATTTGGAGGGAAAAAGAAGAGAGAAGAAAATGCCGGTGGCAAGGCCTGAAACCTCTGATTCCAGAGTCATTGCTCACGTCGACATGGACTGCTTCTACGTTCAAGTGGAGCAAAGGAAACAACCAGATTTAAGGGGCTTACCAACAGCTGTGGTACAGTATAACGCCTGGAAAGGTGGGGCCTTGATTGCTGTCAGCTATGAGGCCCGAAAATGTGGTGTGAAACGTTCGATGCGAGGTTCTGAGTCAAAGCAAGTTTGCCCACAAATTCAGCTGGTCCAAGTCCCAGTAGCACGTGGTAAAGCTGACTTGAGCACATACCGCAATGCGGGATCAGAGGTGGTTTCTATTCTGGCAAGGAAGGGTCGATGTGAGAGGGCATCAATTGACGAAGTGTATCTTGATCTTACTGATGCAGCAGAAACCATGTTAGCGGAAGCTCCTCCCGAGAGCTTGGAAATAATTGAAGAGGAAGCTCTCAAGTCGCATGTTTTGGGGCTTCAAAGCGAGGACAGCAGTGATGCCAAAGAAAATGTGCGAAAATGGCTTTGCAGGAGCAGTGTTGATCGACGTGATAAACTATTAGCTTGTGGGGCACTCATTGTTGCAGAACTCAGGTTGCAAGTGTTGAAGGAGACTGAATTTACTTGTTCAGCTGGCATTGCTCATAATAAGATGCTGGCTAAACTTGCAAGTGGTATGAATAAACCTGCACAACAAACTATTGTTCCGTCCTCATCTGTAAAAGGATTGCTAGATCCCTTGCCAATTAAGAAAATGAAGCAGCTTGGAGGAAAGTTGGGTAATTCATTACAAAGTGACCTGGGTATTAACACTGTTGGTGACCTTTTGCAGTTTTCAGAAGCAAAACTACAAGAACTCTATGGAATCAATACTGGTACCTGGTTATGGAATATTGCTAGAGGGGTCAGTGGGGAAGAAGTTGAGGGACGCCTTCTTCCGAAAAGCCATGGTTCTGGGAAGACATTTCCTGGACCTCAGGCTTTGAAGACGATTGCTTCTGTTCAGCATTGGCTTAATCAACTTTGCGAGGAATTGAGTGAACGTCTTCTATCAGACTTGGACCAGAACAAAAGGATAGCTCACACACTAATGATGCATGGTACTGCATACAAGGCCAGTGATTCAGATTCACATAAGAAGTTTCCTTCGAAGTCTTGTCCCTTAAGGTATGGGACTGCCAAGATACAAGAAGATGCTTTTAGTCTATTTCAAGCTGCATTACGAGAATACTTGGGTTCATACACAGCCAAAATACAAGGAAATCAAAACAACCAATGGGGAATAACGTCACTTTCTGTTTCAGCAAGTAAAATTGTTCCTATACCAACTGGAACAGCTTCAATCACGAAATACTTTCATGGCAATCATACGTCTTGCTCTTCAACAATGCAAACACAGGATACCCTTTTTGAAGAAGCTGATCCTCTGTCACCTGCAGGCATTTCCAGTCTCCTAGTTGTCCTTTGTAAAGAAAGCAACCCGGAGGTGAATGTAACTAAGCCACAGATTGAGTTCCCTGGTGAAGAAACCACGATCAAGCATGCTGTGCCTAGTTTGGATCAACTGGAAGACAAAAAGGATTTGTTGAATGACCAGATTCTCTGTGCTTCAACTGACTATGAGATTGACTCCATTGTGACTCTTCTCTCGTGGCCTCAATTTCCAATCTACAGTTGCTTTACTTTCTTTTTACCCACTTTCTCAATTGTGTTCAATTTAGTTACTTTCCATGGCATTACCTCACTGGCAAATAACACTTATCATATTTTCACTATTTTTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003824 GO:0003887 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006301 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009650 GO:0009987 GO:0010224 GO:0010225 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019985 GO:0031323 GO:0031974 GO:0031981 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042276 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070987 GO:0071704 GO:0071897 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:2001020

KEGG Pathways

Metabolic & Signaling

ko01524 ko03460 map01524 map03460

Pfam Domains

Protein Families

IMS IMS_C

Protein Analysis

669

Amino Acids

74.12

Weight (kDa)

7.52

Isoelectric Point (pI)

48.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IMS	PF00817	93 - 298	2.8e-41	impB/mucB/samB family
POLH-Rev1_HhH	PF21704	321 - 376	2.5e-22	DNApol eta/Rev1, HhH motif
IMS_C	PF11799	387 - 515	3.4e-08	impB/mucB/samB family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002324)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44740 AT5G44740 AT5G44740
fragaria_vesca	FvH4_3g13010 FvH4_3g13010 FvH4_3g13010 FvH4_3g13010
malus_domestica	MD10G1227100.v1.1 MD10G1227200.v1.1
prunus_persica	Prupe.4G118000_v2.0.a1 Prupe.4G118000_v2.0.a1 Prupe.4G118000_v2.0.a1
pyrus_communis	pycom10g19090
rosa_chinensis	RchiOBHm_Chr4g0406911 RchiOBHm_Chr4g0406921 RchiOBHm_Chr5g0021111 RchiOBHm_Chr5g0021121 RchiOBHm_Chr5g0021171 RchiOBHm_Chr5g0021201
rosa_laevigata	RLG00000032590 RLG00000032594
rosa_multiflora	Rmu_sc0002467.1_g000007 Rmu_sc0002467.1_g000009 Rmu_sc0002467.1_g000013 Rmu_sc0002467.1_g000015 Rmu_sc0012431.1_g000002
rosa_roxburghii	Rroxscaffold_1G00057300 Rroxscaffold_1G00057320 Rroxscaffold_1G00057330 Rroxscaffold_5G00351670
rosa_rugosa	Rorug05G0064800 Rorug05G0065100 Rorug05G0065100
rosa_samantha	Rh5AG154000 Rh5BG153700 Rh5CG166700 Rh5CG167300 Rh5DG154100
rosa_wichuraiana	Rw1G022470 Rw5G013690 Rw5G013710 Rw5G013730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1663
Acc36I	ACCTGC	2 cut(s)	916, 1663
Acc65I	GGTACC	1 cut(s)	1097
AccB1I	GGYRCC	1 cut(s)	1097
AccI	GTMKAC	1 cut(s)	279
AciI	CCGC	3 cut(s)	520, 527, 632
AclI	AACGTT	1 cut(s)	424
AclWI	GGATC	4 cut(s)	538, 950, 1637, 1788
AcsI	RAATTY	2 cut(s)	465, 839
AcvI	CACGTG	1 cut(s)	494
AcyI	GRCGYC	1 cut(s)	1150
AfaI	GTAC	4 cut(s)	139, 354, 1099, 1324
AfiI	CCNNNNNNNGG	2 cut(s)	526, 1107
AflII	CTTAAG	1 cut(s)	1384
AjiI	CACGTC	2 cut(s)	277, 770
AjnI	CCWGG	5 cut(s)	365, 1020, 1100, 1189, 1738
AjuI	GAANNNNNNNTTGG	2 cut(s)	185, 217
Alw21I	GWGCWC	2 cut(s)	122, 515
Alw26I	GTCTC	2 cut(s)	827, 1676
Alw44I	GTGCAC	1 cut(s)	118
AlwI	GGATC	4 cut(s)	538, 950, 1637, 1788
Ama87I	CYCGRG	1 cut(s)	644
AoxI	GGCC	6 cut(s)	39, 242, 378, 402, 1335, 1874
ApaI	GGGCCC	1 cut(s)	43
ApaLI	GTGCAC	1 cut(s)	118
ApeKI	GCWGC	3 cut(s)	614, 982, 1436
ApoI	RAATTY	2 cut(s)	465, 839
Asp700I	GAANNNNTTC	2 cut(s)	1266, 1558
Asp718I	GGTACC	1 cut(s)	1097
AspS9I	GGNCC	7 cut(s)	39, 40, 378, 403, 475, 1193, 1282
AsuC2I	CCSGG	1 cut(s)	1703
AsuHPI	GGTGA	4 cut(s)	1052, 1644, 1720, 1754
AsuII	TTCGAA	1 cut(s)	1370
AvaI	CYCGRG	1 cut(s)	644
AvaII	GGWCC	3 cut(s)	475, 1193, 1282
AxyI	CCTNAGG	1 cut(s)	1197
BaeGI	GKGCMC	3 cut(s)	43, 122, 796
BanI	GGYRCC	1 cut(s)	1097
BanII	GRGCYC	1 cut(s)	43
BarI	GAAGNNNNNNTAC	2 cut(s)	1053, 1085
BauI	CACGAG	1 cut(s)	1870
BbrPI	CACGTG	1 cut(s)	494
Bbv12I	GWGCWC	2 cut(s)	122, 515
BbvI	GCAGC	3 cut(s)	626, 994, 1423
BciT130I	CCWGG	5 cut(s)	367, 1022, 1102, 1191, 1740
BciVI	GTATCC	1 cut(s)	1615
BcnI	CCSGG	1 cut(s)	1703
BcoDI	GTCTC	2 cut(s)	827, 1676
BfaI	CTAG	4 cut(s)	953, 1119, 1676, 1773
BfmI	CTRYAG	3 cut(s)	163, 1656, 1891
BfrI	CTTAAG	1 cut(s)	1384
BfuAI	ACCTGC	2 cut(s)	916, 1663
BfuI	GTATCC	1 cut(s)	1615
BisI	GCNGC	3 cut(s)	615, 983, 1437
BlsI	GCNGC	3 cut(s)	616, 984, 1438
Bme1390I	CCNGG	6 cut(s)	367, 1022, 1102, 1191, 1703, 1740
Bme18I	GGWCC	3 cut(s)	475, 1193, 1282
BmeT110I	CYCGRG	1 cut(s)	644
BmgBI	CACGTC	2 cut(s)	277, 770
BmgT120I	GGNCC	7 cut(s)	39, 40, 378, 403, 475, 1193, 1282
BmiI	GGNNCC	3 cut(s)	41, 379, 1099
BmrFI	CCNGG	6 cut(s)	367, 1022, 1102, 1191, 1703, 1740
BmrI	ACTGGG	1 cut(s)	479
BmsI	GCATC	7 cut(s)	420, 587, 601, 709, 864, 1305, 1405
BmuI	ACTGGG	1 cut(s)	479
BpiI	GAAGAC	4 cut(s)	1187, 1214, 1259, 1797
BplI	GAGNNNNNCTC	2 cut(s)	1835, 1867
Bpu14I	TTCGAA	1 cut(s)	1370
BpuEI	CTTGAG	2 cut(s)	529, 662
BpuMI	CCSGG	1 cut(s)	1703
BsaAI	YACGTR	1 cut(s)	494
BsaHI	GRCGYC	1 cut(s)	1150
BsaJI	CCNNGG	4 cut(s)	1021, 1168, 1738, 1951
Bsc4I	CCNNNNNNNGG	2 cut(s)	526, 1107
Bse118I	RCCGGY	2 cut(s)	174, 232
Bse1I	ACTGG	8 cut(s)	191, 485, 1099, 1338, 1555, 1668, 1791, 1971
Bse21I	CCTNAGG	1 cut(s)	1197
Bse3DI	GCAATG	4 cut(s)	101, 267, 529, 858
BseBI	CCWGG	5 cut(s)	367, 1022, 1102, 1191, 1740
BseDI	CCNNGG	4 cut(s)	1021, 1168, 1738, 1951
BseLI	CCNNNNNNNGG	2 cut(s)	526, 1107
BseMI	GCAATG	4 cut(s)	101, 267, 529, 858
BseMII	CTCAG	3 cut(s)	432, 826, 1211
BseNI	ACTGG	8 cut(s)	191, 485, 1099, 1338, 1555, 1668, 1791, 1971
BseRI	GAGGAG	1 cut(s)	630
BseSI	GKGCMC	3 cut(s)	43, 122, 796
BseXI	GCAGC	3 cut(s)	626, 994, 1423
BsgI	GTGCAG	1 cut(s)	894
Bsh1285I	CGRYCG	1 cut(s)	179
BshFI	GGCC	6 cut(s)	41, 244, 380, 404, 1337, 1876
BshNI	GGYRCC	1 cut(s)	1097
BsiEI	CGRYCG	1 cut(s)	179
BsiHKAI	GWGCWC	2 cut(s)	122, 515
BsiHKCI	CYCGRG	1 cut(s)	644
BsiSI	CCGG	3 cut(s)	175, 233, 1703
BsiWI	CGTACG	1 cut(s)	137
BslFI	GGGAC	4 cut(s)	467, 1161, 1365, 1408
BslI	CCNNNNNNNGG	2 cut(s)	526, 1107
BsmAI	GTCTC	2 cut(s)	827, 1676
BsmFI	GGGAC	4 cut(s)	467, 1161, 1365, 1408
BsmI	GAATGC	1 cut(s)	70
BsnI	GGCC	6 cut(s)	41, 244, 380, 404, 1337, 1876
BsoBI	CYCGRG	1 cut(s)	644
Bsp119I	TTCGAA	1 cut(s)	1370
Bsp120I	GGGCCC	1 cut(s)	39
Bsp1286I	GDGCHC	4 cut(s)	43, 122, 515, 796
Bsp143I	GATC	7 cut(s)	530, 601, 763, 955, 1642, 1755, 1780
Bsp19I	CCATGG	2 cut(s)	1168, 1951
BspACI	CCGC	3 cut(s)	520, 527, 632
BspANI	GGCC	6 cut(s)	41, 244, 380, 404, 1337, 1876
BspCNI	CTCAG	3 cut(s)	433, 825, 1210
BspLI	GGNNCC	3 cut(s)	41, 379, 1099
BspMAI	CTGCAG	1 cut(s)	1660
BspMI	ACCTGC	2 cut(s)	916, 1663
BspPI	GGATC	4 cut(s)	538, 950, 1637, 1788
BspQI	GCTCTTC	1 cut(s)	1606
BspT104I	TTCGAA	1 cut(s)	1370
BspT107I	GGYRCC	1 cut(s)	1097
BspTI	CTTAAG	1 cut(s)	1384
BsrDI	GCAATG	4 cut(s)	101, 267, 529, 858
BsrFI	RCCGGY	2 cut(s)	174, 232
BsrI	ACTGG	8 cut(s)	191, 485, 1099, 1338, 1555, 1668, 1791, 1971
BssAI	RCCGGY	2 cut(s)	174, 232
BssECI	CCNNGG	4 cut(s)	1021, 1168, 1738, 1951
BssMI	GATC	7 cut(s)	530, 601, 763, 955, 1642, 1755, 1780
BssNI	GRCGYC	1 cut(s)	1150
BssSI	CACGAG	1 cut(s)	1870
BssT1I	CCWWGG	2 cut(s)	1168, 1951
Bst2BI	CACGAG	1 cut(s)	1870
Bst2UI	CCWGG	5 cut(s)	367, 1022, 1102, 1191, 1740
Bst4CI	ACNGT	4 cut(s)	167, 357, 1036, 1895
Bst6I	CTCTTC	4 cut(s)	211, 660, 1606, 1869
BstACI	GRCGYC	1 cut(s)	1150
BstAFI	CTTAAG	1 cut(s)	1384
BstAPI	GCANNNNNTGC	1 cut(s)	1768
BstBAI	YACGTR	1 cut(s)	494
BstBI	TTCGAA	1 cut(s)	1370
BstC8I	GCNNGC	4 cut(s)	856, 879, 1660, 1764
BstDEI	CTNAG	4 cut(s)	441, 812, 1197, 1719
BstDSI	CCRYGG	2 cut(s)	1168, 1951
BstEII	GGTNACC	1 cut(s)	1040
BstKTI	GATC	7 cut(s)	533, 604, 766, 958, 1645, 1758, 1783
BstMAI	GTCTC	2 cut(s)	827, 1676
BstMBI	GATC	7 cut(s)	530, 601, 763, 955, 1642, 1755, 1780
BstMCI	CGRYCG	1 cut(s)	179
BstMWI	GCNNNNNNNGC	9 cut(s)	386, 519, 711, 742, 791, 860, 887, 1334, 1768
BstNI	CCWGG	5 cut(s)	367, 1022, 1102, 1191, 1740
BstNSI	RCATGY	2 cut(s)	689, 1766
BstPI	GGTNACC	1 cut(s)	1040
BstSCI	CCNGG	6 cut(s)	365, 1020, 1100, 1189, 1701, 1738
BstSFI	CTRYAG	3 cut(s)	163, 1656, 1891
BstSLI	GKGCMC	3 cut(s)	43, 122, 796
BstV1I	GCAGC	3 cut(s)	626, 994, 1423
BstV2I	GAAGAC	4 cut(s)	1187, 1214, 1259, 1797
BstX2I	RGATCY	1 cut(s)	955
BstXI	CCANNNNNNTGG	1 cut(s)	1175
BstYI	RGATCY	1 cut(s)	955
Bsu36I	CCTNAGG	1 cut(s)	1197
BsuI	GTATCC	1 cut(s)	1615
BsuRI	GGCC	6 cut(s)	41, 244, 380, 404, 1337, 1876
BtgI	CCRYGG	2 cut(s)	1168, 1951
BtrI	CACGTC	2 cut(s)	277, 770
BtsI	GCAGTG	2 cut(s)	721, 763
BtsIMutI	CAGTG	6 cut(s)	721, 763, 1032, 1135, 1345, 1964
BveI	ACCTGC	2 cut(s)	916, 1663
Cac8I	GCNNGC	4 cut(s)	856, 879, 1660, 1764
Cfr10I	RCCGGY	2 cut(s)	174, 232
Cfr13I	GGNCC	7 cut(s)	39, 40, 378, 403, 475, 1193, 1282
CseI	GACGC	1 cut(s)	1158
CsiI	ACCWGGT	1 cut(s)	1100
Csp6I	GTAC	4 cut(s)	138, 353, 1098, 1323
CspCI	CAANNNNNGTGG	2 cut(s)	330, 365
CviAII	CATG	8 cut(s)	283, 625, 686, 1169, 1319, 1580, 1763, 1952
CviQI	GTAC	4 cut(s)	138, 353, 1098, 1323
DdeI	CTNAG	4 cut(s)	441, 812, 1197, 1719
DpnI	GATC	7 cut(s)	532, 603, 765, 957, 1644, 1757, 1782
DpnII	GATC	7 cut(s)	530, 601, 763, 955, 1642, 1755, 1780
Eam1104I	CTCTTC	4 cut(s)	211, 660, 1606, 1869
EarI	CTCTTC	4 cut(s)	211, 660, 1606, 1869
Eco130I	CCWWGG	2 cut(s)	1168, 1951
Eco147I	AGGCCT	1 cut(s)	244
Eco24I	GRGCYC	1 cut(s)	43
Eco47I	GGWCC	3 cut(s)	475, 1193, 1282
Eco72I	CACGTG	1 cut(s)	494
Eco81I	CCTNAGG	1 cut(s)	1197
Eco88I	CYCGRG	1 cut(s)	644
Eco91I	GGTNACC	1 cut(s)	1040
EcoO109I	RGGNCCY	1 cut(s)	378
EcoO65I	GGTNACC	1 cut(s)	1040
EcoRII	CCWGG	5 cut(s)	365, 1020, 1100, 1189, 1738
EcoT14I	CCWWGG	2 cut(s)	1168, 1951
EcoT22I	ATGCAT	1 cut(s)	1320
EcoT38I	GRGCYC	1 cut(s)	43
ErhI	CCWWGG	2 cut(s)	1168, 1951
FaeI	CATG	8 cut(s)	286, 628, 689, 1172, 1322, 1583, 1766, 1955
FalI	AAGNNNNNCTT	2 cut(s)	582, 614
FaqI	GGGAC	4 cut(s)	467, 1161, 1365, 1408
FatI	CATG	8 cut(s)	282, 624, 685, 1168, 1318, 1579, 1762, 1951
FauI	CCCGC	1 cut(s)	520
FblI	GTMKAC	1 cut(s)	279
Fnu4HI	GCNGC	3 cut(s)	615, 983, 1437
FriOI	GRGCYC	1 cut(s)	43
Fsp4HI	GCNGC	3 cut(s)	615, 983, 1437
FspBI	CTAG	4 cut(s)	953, 1119, 1676, 1773
GluI	GCNGC	3 cut(s)	615, 983, 1437
HaeIII	GGCC	6 cut(s)	41, 244, 380, 404, 1337, 1876
HapII	CCGG	3 cut(s)	175, 233, 1703
HgaI	GACGC	1 cut(s)	1158
Hin1I	GRCGYC	1 cut(s)	1150
Hin1II	CATG	8 cut(s)	286, 628, 689, 1172, 1322, 1583, 1766, 1955
HincII	GTYRAC	1 cut(s)	280
HindII	GTYRAC	1 cut(s)	280
HindIII	AAGCTT	1 cut(s)	72
HinfI	GANTC	9 cut(s)	257, 264, 443, 1086, 1343, 1349, 1819, 1850, 1861
HpaII	CCGG	3 cut(s)	175, 233, 1703
HphI	GGTGA	4 cut(s)	1052, 1644, 1720, 1754
Hpy166II	GTNNAC	3 cut(s)	120, 280, 1262
Hpy188I	TCNGA	9 cut(s)	13, 63, 256, 442, 535, 1060, 1161, 1276, 1348
Hpy188III	TCNNGA	4 cut(s)	261, 599, 644, 1567
Hpy8I	GTNNAC	3 cut(s)	120, 280, 1262
Hpy99I	CGWCG	2 cut(s)	281, 771
HpyAV	CCTTC	4 cut(s)	553, 823, 1163, 1377
HpyCH4III	ACNGT	4 cut(s)	167, 357, 1036, 1895
HpyF10VI	GCNNNNNNNGC	9 cut(s)	386, 519, 711, 742, 791, 860, 887, 1334, 1768
HpyF3I	CTNAG	4 cut(s)	441, 812, 1197, 1719
Hsp92I	GRCGYC	1 cut(s)	1150
Hsp92II	CATG	8 cut(s)	286, 628, 689, 1172, 1322, 1583, 1766, 1955
KpnI	GGTACC	1 cut(s)	1101
Kzo9I	GATC	7 cut(s)	530, 601, 763, 955, 1642, 1755, 1780
LguI	GCTCTTC	1 cut(s)	1606
LmnI	GCTCC	3 cut(s)	307, 643, 753
Lsp1109I	GCAGC	3 cut(s)	626, 994, 1423
LweI	GCATC	7 cut(s)	420, 587, 601, 709, 864, 1305, 1405
MabI	ACCWGGT	1 cut(s)	1100
MaeI	CTAG	4 cut(s)	953, 1119, 1676, 1773
MaeIII	GTNAC	7 cut(s)	1016, 1040, 1509, 1650, 1714, 1858, 1942
MalI	GATC	7 cut(s)	532, 603, 765, 957, 1644, 1757, 1782
MboI	GATC	7 cut(s)	530, 601, 763, 955, 1642, 1755, 1780
MfeI	CAATTG	2 cut(s)	582, 1926
MflI	RGATCY	1 cut(s)	955
MhlI	GDGCHC	4 cut(s)	43, 122, 515, 796
MlyI	GAGTC	4 cut(s)	273, 452, 1844, 1855
Mph1103I	ATGCAT	1 cut(s)	1320
MroXI	GAANNNNTTC	2 cut(s)	1266, 1558
MseI	TTAA	5 cut(s)	329, 969, 1029, 1236, 1385
MslI	CAYNNNNRTG	1 cut(s)	1310
MspA1I	CMGCKG	3 cut(s)	347, 472, 854
MspCI	CTTAAG	1 cut(s)	1384
MspI	CCGG	3 cut(s)	175, 233, 1703
MspR9I	CCNGG	6 cut(s)	367, 1022, 1102, 1191, 1703, 1740
MunI	CAATTG	2 cut(s)	582, 1926
Mva1269I	GAATGC	1 cut(s)	70
MvaI	CCWGG	5 cut(s)	367, 1022, 1102, 1191, 1740
MwoI	GCNNNNNNNGC	9 cut(s)	386, 519, 711, 742, 791, 860, 887, 1334, 1768
NciI	CCSGG	1 cut(s)	1703
NcoI	CCATGG	2 cut(s)	1168, 1951
NdeII	GATC	7 cut(s)	530, 601, 763, 955, 1642, 1755, 1780
NlaIII	CATG	8 cut(s)	286, 628, 689, 1172, 1322, 1583, 1766, 1955
NlaIV	GGNNCC	3 cut(s)	41, 379, 1099
NmuCI	GTSAC	5 cut(s)	1016, 1040, 1509, 1650, 1858
NsiI	ATGCAT	1 cut(s)	1320
NspI	RCATGY	2 cut(s)	689, 1766
NspV	TTCGAA	1 cut(s)	1370
PaeI	GCATGC	1 cut(s)	1766
PaqCI	CACCTGC	1 cut(s)	1663
PceI	AGGCCT	1 cut(s)	244
PciSI	GCTCTTC	1 cut(s)	1606
PcsI	WCGNNNNNNNCGW	1 cut(s)	430
PctI	GAATGC	1 cut(s)	70
PdmI	GAANNNNTTC	2 cut(s)	1266, 1558
PfeI	GAWTC	5 cut(s)	257, 1086, 1343, 1349, 1819
Pfl23II	CGTACG	1 cut(s)	137
PfoI	TCCNGGA	1 cut(s)	1189
PkrI	GCNGC	3 cut(s)	616, 984, 1438
PleI	GAGTC	4 cut(s)	272, 451, 1844, 1855
PmaCI	CACGTG	1 cut(s)	494
PmlI	CACGTG	1 cut(s)	494
PpsI	GAGTC	4 cut(s)	272, 451, 1844, 1855
Ppu21I	YACGTR	1 cut(s)	494
Psp1406I	AACGTT	1 cut(s)	424
Psp6I	CCWGG	5 cut(s)	365, 1020, 1100, 1189, 1738
PspCI	CACGTG	1 cut(s)	494
PspEI	GGTNACC	1 cut(s)	1040
PspGI	CCWGG	5 cut(s)	365, 1020, 1100, 1189, 1738
PspLI	CGTACG	1 cut(s)	137
PspN4I	GGNNCC	3 cut(s)	41, 379, 1099
PspOMI	GGGCCC	1 cut(s)	39
PspPI	GGNCC	7 cut(s)	39, 40, 378, 403, 475, 1193, 1282
PstI	CTGCAG	1 cut(s)	1660
PsuI	RGATCY	1 cut(s)	955
PvuII	CAGCTG	3 cut(s)	347, 472, 854
RsaI	GTAC	4 cut(s)	139, 354, 1099, 1324
RsaNI	GTAC	4 cut(s)	138, 353, 1098, 1323
RseI	CAYNNNNRTG	1 cut(s)	1310
SalI	GTCGAC	1 cut(s)	278
SapI	GCTCTTC	1 cut(s)	1606
SaqAI	TTAA	5 cut(s)	329, 969, 1029, 1236, 1385
SatI	GCNGC	3 cut(s)	615, 983, 1437
Sau3AI	GATC	7 cut(s)	530, 601, 763, 955, 1642, 1755, 1780
Sau96I	GGNCC	7 cut(s)	39, 40, 378, 403, 475, 1193, 1282
SbfI	CCTGCAGG	1 cut(s)	1660
SchI	GAGTC	4 cut(s)	273, 452, 1844, 1855
ScrFI	CCNGG	6 cut(s)	367, 1022, 1102, 1191, 1703, 1740
SdaI	CCTGCAGG	1 cut(s)	1660
SduI	GDGCHC	4 cut(s)	43, 122, 515, 796
SexAI	ACCWGGT	1 cut(s)	1100
SfaNI	GCATC	7 cut(s)	420, 587, 601, 709, 864, 1305, 1405
SfcI	CTRYAG	3 cut(s)	163, 1656, 1891
SfuI	TTCGAA	1 cut(s)	1370
SinI	GGWCC	3 cut(s)	475, 1193, 1282
SmiMI	CAYNNNNRTG	1 cut(s)	1310
SmlI	CTYRAG	3 cut(s)	508, 677, 1384
SmoI	CTYRAG	3 cut(s)	508, 677, 1384
SphI	GCATGC	1 cut(s)	1766
Sse8387I	CCTGCAGG	1 cut(s)	1660
SseBI	AGGCCT	1 cut(s)	244
SsiI	CCGC	3 cut(s)	520, 527, 632
SspI	AATATT	2 cut(s)	31, 1114
SspMI	CTAG	4 cut(s)	953, 1119, 1676, 1773
StuI	AGGCCT	1 cut(s)	244
StyD4I	CCNGG	6 cut(s)	365, 1020, 1100, 1189, 1701, 1738
StyI	CCWWGG	2 cut(s)	1168, 1951
TaaI	ACNGT	4 cut(s)	167, 357, 1036, 1895
TaqI	TCGA	5 cut(s)	279, 428, 565, 766, 1370
TfiI	GAWTC	5 cut(s)	257, 1086, 1343, 1349, 1819
Tru1I	TTAA	5 cut(s)	329, 969, 1029, 1236, 1385
Tru9I	TTAA	5 cut(s)	329, 969, 1029, 1236, 1385
TscAI	CASTG	6 cut(s)	721, 763, 1039, 1135, 1345, 1971
TseFI	GTSAC	5 cut(s)	1016, 1040, 1509, 1650, 1858
TseI	GCWGC	3 cut(s)	614, 982, 1436
Tsp45I	GTSAC	5 cut(s)	1016, 1040, 1509, 1650, 1858
TspDTI	ATGAA	6 cut(s)	17, 914, 992, 996, 1449, 1568
TspGWI	ACGGA	1 cut(s)	918
TspRI	CASTG	6 cut(s)	721, 763, 1039, 1135, 1345, 1971
Vha464I	CTTAAG	1 cut(s)	1384
VneI	GTGCAC	1 cut(s)	118
VpaK11BI	GGWCC	3 cut(s)	475, 1193, 1282
XapI	RAATTY	2 cut(s)	465, 839
XceI	RCATGY	2 cut(s)	689, 1766
XmiI	GTMKAC	1 cut(s)	279
XmnI	GAANNNNTTC	2 cut(s)	1266, 1558
XspI	CTAG	4 cut(s)	953, 1119, 1676, 1773
Zsp2I	ATGCAT	1 cut(s)	1320

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.