Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001177

Physical Location & Seq

Genomic Coordinates Reverse (-)

4161414 .. 4162100

Gene Structure 687 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1038600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 687 bp

ATGTCAGAGAAACAAGCCCATTTGAACGGGGCCTACTACGGCCCCTCAATCCCTCCCAAATCCCAGTCCTACCACCGCCCTGGCCGCGGCGGCAGCGGATGCCTCGGCTGCTGCTGCAGCTGCATCTTCGGCCTTGTTTTCAAGCTCATCATGACCGCCGTTGTCATCCTGGGCTTGGCGTTTTTCATCTTTTGGCTCATCGTCCGCCCTAACCGTGTCAAGTTCCACGTCACGGACGCCACCCTCACGCAGTTCAACTTCTCCAACGACAACACCCTCCACTACGACCTCGCCCTCAACCTCACCATCCGAAACCCTAACAAGAAGATCGGTATTTACTACGATCGCATCGAGACCCGGGCCTTTTACGAGGATCAAAGGTTCAGCACGGTTACCTTGACGCCCTTTTACCAAGGGCATAAGAAAACGAACGTGTTGAACCCCGTGTTCCAAGGCCAGCAAGTGCTTGTAGACTCGAAGGCGCTTTCAGAGTACAATCAGCAGAAGAGTACTGGGATTTACGAGATCGATTTGAAGATTTATCTTCGGATTCGGTTCAAACTCGGATTGATCAAGACTGGCAAGTTCAAGCCCAAGATTGAATGCGACTTGAAGGTTCCCTTGACTCAGAACGGAAATTCAGTTGGCACTTTCCAGACTACCAAGTGCAAAGTTGATTACTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LEA_2

Protein Analysis

229

Amino Acids

25.98

Weight (kDa)

9.51

Isoelectric Point (pI)

31.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LEA_2	PF03168	101 - 202	9.5e-12	Late embryogenesis abundant protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001177)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35460 AT2G35980 AT3G11650 AT5G06320
fragaria_vesca	FvH4_6g20130 FvH4_6g20140
malus_domestica	MD12G1038600.v1.1 MD14G1037300.v1.1
prunus_persica	Prupe.6G357300_v2.0.a1 Prupe.7G097200_v2.0.a1
pyrus_communis	pycom14g03260
rosa_chinensis	RchiOBHm_Chr3g0475591 RchiOBHm_Chr3g0475631 RchiOBHm_Chr3g0475641 RchiOBHm_Chr3g0475651 RchiOBHm_Chr3g0475661
rosa_laevigata	RLG00000023825 RLG00000023826 RLG00000023827 RLG00000023828 RLG00000023830
rosa_multiflora	Rmu_co8195028.1_g000001 Rmu_sc0000539.1_g000033 Rmu_sc0000539.1_g000043 Rmu_sc0000539.1_g000048 Rmu_sc0000539.1_g000055
rosa_roxburghii	Rroxscaffold_6G00406030 Rroxscaffold_6G00406040 Rroxscaffold_6G00406070 Rroxscaffold_6G00406080 Rroxscaffold_6G00406100 Rroxscaffold_6G00406110
rosa_rugosa	Rorug03G0150500 Rorug03G0150500 Rorug03G0150600 Rorug03G0150700 Rorug03G0150800 Rorug03G0150800
rosa_samantha	Rh3AG200100 Rh3AG200200 Rh3AG200300 Rh3BG230500 Rh3BG230600 Rh3BG230700 Rh3CG224800 Rh3CG225000 Rh3CG225100 Rh3CG225200 Rh3CG225300 Rh3DG225600 Rh3DG225700 Rh3DG225800
rosa_wichuraiana	Rw3G018150 Rw3G018170 Rw3G018180 Rw3G018190 Rw3G018200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	471
AccII	CGCG	1 cut(s)	87
AciI	CCGC	7 cut(s)	76, 85, 87, 90, 96, 156, 205
AclWI	GGATC	1 cut(s)	381
AcoI	YGGCCR	1 cut(s)	82
AcsI	RAATTY	1 cut(s)	637
AcyI	GRCGYC	2 cut(s)	237, 401
AfaI	GTAC	2 cut(s)	494, 511
AfiI	CCNNNNNNNGG	3 cut(s)	86, 175, 232
AflIII	ACRYGT	1 cut(s)	432
AgsI	TTSAA	9 cut(s)	25, 142, 256, 439, 535, 559, 589, 602, 613
AjiI	CACGTC	1 cut(s)	229
AjnI	CCWGG	2 cut(s)	79, 168
AloI	GAACNNNNNNTCC	2 cut(s)	365, 397
AluBI	AGCT	2 cut(s)	120, 145
AluI	AGCT	2 cut(s)	120, 145
Alw26I	GTCTC	1 cut(s)	347
AlwI	GGATC	1 cut(s)	381
Ama87I	CYCGRG	1 cut(s)	357
AoxI	GGCC	6 cut(s)	30, 40, 82, 130, 360, 454
ApeKI	GCWGC	6 cut(s)	93, 108, 111, 114, 117, 120
ApoI	RAATTY	1 cut(s)	637
AspLEI	GCGC	1 cut(s)	484
AspS9I	GGNCC	3 cut(s)	30, 41, 360
AsuC2I	CCSGG	2 cut(s)	358, 359
AsuHPI	GGTGA	1 cut(s)	295
AvaI	CYCGRG	1 cut(s)	357
BbvI	GCAGC	6 cut(s)	95, 98, 101, 105, 107, 129
BccI	CCATC	1 cut(s)	314
BceAI	ACGGC	2 cut(s)	55, 143
BciT130I	CCWGG	2 cut(s)	81, 170
BclI	TGATCA	1 cut(s)	570
BcnI	CCSGG	2 cut(s)	358, 359
BcoDI	GTCTC	1 cut(s)	347
BfmI	CTRYAG	1 cut(s)	115
BfoI	RGCGCY	1 cut(s)	485
BglI	GCCNNNNNGGC	1 cut(s)	90
BisI	GCNGC	9 cut(s)	85, 88, 91, 94, 109, 112, 115, 118, 121
BlsI	GCNGC	9 cut(s)	86, 89, 92, 95, 110, 113, 116, 119, 122
BmcAI	AGTACT	1 cut(s)	511
Bme1390I	CCNGG	4 cut(s)	81, 170, 358, 359
BmeT110I	CYCGRG	1 cut(s)	357
BmgBI	CACGTC	1 cut(s)	229
BmgT120I	GGNCC	3 cut(s)	30, 41, 360
BmiI	GGNNCC	3 cut(s)	31, 43, 618
BmrFI	CCNGG	4 cut(s)	81, 170, 358, 359
BmrI	ACTGGG	2 cut(s)	58, 522
BmsI	GCATC	3 cut(s)	89, 132, 357
BmuI	ACTGGG	2 cut(s)	58, 522
BpuMI	CCSGG	2 cut(s)	358, 359
Bsa29I	ATCGAT	1 cut(s)	528
BsaHI	GRCGYC	2 cut(s)	237, 401
BsaI	GGTCTC	1 cut(s)	347
BsaJI	CCNNGG	7 cut(s)	79, 85, 103, 169, 357, 412, 451
Bsc4I	CCNNNNNNNGG	3 cut(s)	86, 175, 232
Bse1I	ACTGG	3 cut(s)	64, 517, 583
BseBI	CCWGG	2 cut(s)	81, 170
BseCI	ATCGAT	1 cut(s)	528
BseDI	CCNNGG	7 cut(s)	79, 85, 103, 169, 357, 412, 451
BseGI	GGATG	3 cut(s)	104, 165, 306
BseLI	CCNNNNNNNGG	3 cut(s)	86, 175, 232
BseMII	CTCAG	1 cut(s)	641
BseNI	ACTGG	3 cut(s)	64, 517, 583
BseXI	GCAGC	6 cut(s)	95, 98, 101, 105, 107, 129
Bsh1236I	CGCG	1 cut(s)	87
Bsh1285I	CGRYCG	1 cut(s)	346
BshFI	GGCC	6 cut(s)	32, 42, 84, 132, 362, 456
BshVI	ATCGAT	1 cut(s)	528
BsiEI	CGRYCG	1 cut(s)	346
BsiHKCI	CYCGRG	1 cut(s)	357
BsiSI	CCGG	1 cut(s)	358
BslI	CCNNNNNNNGG	3 cut(s)	86, 175, 232
BsmAI	GTCTC	1 cut(s)	347
BsmI	GAATGC	1 cut(s)	608
BsnI	GGCC	6 cut(s)	32, 42, 84, 132, 362, 456
Bso31I	GGTCTC	1 cut(s)	347
BsoBI	CYCGRG	1 cut(s)	357
Bsp143I	GATC	5 cut(s)	327, 343, 373, 525, 570
BspACI	CCGC	7 cut(s)	76, 85, 87, 90, 96, 156, 205
BspANI	GGCC	6 cut(s)	32, 42, 84, 132, 362, 456
BspCNI	CTCAG	1 cut(s)	640
BspDI	ATCGAT	1 cut(s)	528
BspFNI	CGCG	1 cut(s)	87
BspHI	TCATGA	1 cut(s)	150
BspLI	GGNNCC	3 cut(s)	31, 43, 618
BspMAI	CTGCAG	1 cut(s)	119
BspPI	GGATC	1 cut(s)	381
BspTNI	GGTCTC	1 cut(s)	347
BsrI	ACTGG	3 cut(s)	64, 517, 583
BssECI	CCNNGG	7 cut(s)	79, 85, 103, 169, 357, 412, 451
BssMI	GATC	5 cut(s)	327, 343, 373, 525, 570
BssNI	GRCGYC	2 cut(s)	237, 401
BssT1I	CCWWGG	2 cut(s)	412, 451
Bst2UI	CCWGG	2 cut(s)	81, 170
Bst4CI	ACNGT	2 cut(s)	215, 391
Bst6I	CTCTTC	1 cut(s)	500
BstACI	GRCGYC	2 cut(s)	237, 401
BstAPI	GCANNNNNTGC	1 cut(s)	99
BstC8I	GCNNGC	1 cut(s)	458
BstDEI	CTNAG	1 cut(s)	627
BstDSI	CCRYGG	1 cut(s)	85
BstEII	GGTNACC	1 cut(s)	391
BstF5I	GGATG	3 cut(s)	104, 165, 306
BstFNI	CGCG	1 cut(s)	87
BstH2I	RGCGCY	1 cut(s)	485
BstHHI	GCGC	1 cut(s)	484
BstKTI	GATC	5 cut(s)	330, 346, 376, 528, 573
BstMAI	GTCTC	1 cut(s)	347
BstMBI	GATC	5 cut(s)	327, 343, 373, 525, 570
BstMCI	CGRYCG	1 cut(s)	346
BstMWI	GCNNNNNNNGC	9 cut(s)	84, 90, 93, 99, 108, 114, 117, 120, 129
BstNI	CCWGG	2 cut(s)	81, 170
BstPI	GGTNACC	1 cut(s)	391
BstSCI	CCNGG	4 cut(s)	79, 168, 356, 357
BstSFI	CTRYAG	1 cut(s)	115
BstUI	CGCG	1 cut(s)	87
BstV1I	GCAGC	6 cut(s)	95, 98, 101, 105, 107, 129
BstXI	CCANNNNNNTGG	1 cut(s)	80
Bsu15I	ATCGAT	1 cut(s)	528
BsuRI	GGCC	6 cut(s)	32, 42, 84, 132, 362, 456
BsuTUI	ATCGAT	1 cut(s)	528
BtgI	CCRYGG	1 cut(s)	85
BtrI	CACGTC	1 cut(s)	229
BtsCI	GGATG	3 cut(s)	104, 165, 306
Cac8I	GCNNGC	1 cut(s)	458
CciI	TCATGA	1 cut(s)	150
CfoI	GCGC	1 cut(s)	484
Cfr13I	GGNCC	3 cut(s)	30, 41, 360
Cfr42I	CCGCGG	1 cut(s)	88
Cfr9I	CCCGGG	1 cut(s)	357
ClaI	ATCGAT	1 cut(s)	528
CseI	GACGC	2 cut(s)	245, 409
Csp6I	GTAC	2 cut(s)	493, 510
CviAII	CATG	1 cut(s)	151
CviQI	GTAC	2 cut(s)	493, 510
DdeI	CTNAG	1 cut(s)	627
DpnI	GATC	5 cut(s)	329, 345, 375, 527, 572
DpnII	GATC	5 cut(s)	327, 343, 373, 525, 570
EaeI	YGGCCR	1 cut(s)	82
Eam1104I	CTCTTC	1 cut(s)	500
EarI	CTCTTC	1 cut(s)	500
EciI	GGCGGA	1 cut(s)	194
Eco130I	CCWWGG	2 cut(s)	412, 451
Eco31I	GGTCTC	1 cut(s)	347
Eco88I	CYCGRG	1 cut(s)	357
Eco91I	GGTNACC	1 cut(s)	391
EcoO109I	RGGNCCY	1 cut(s)	30
EcoO65I	GGTNACC	1 cut(s)	391
EcoRII	CCWGG	2 cut(s)	79, 168
EcoT14I	CCWWGG	2 cut(s)	412, 451
ErhI	CCWWGG	2 cut(s)	412, 451
FaeI	CATG	1 cut(s)	154
FaiI	YATR	2 cut(s)	152, 420
FalI	AAGNNNNNCTT	2 cut(s)	605, 637
FatI	CATG	1 cut(s)	150
FbaI	TGATCA	1 cut(s)	570
FblI	GTMKAC	1 cut(s)	471
Fnu4HI	GCNGC	9 cut(s)	85, 88, 91, 94, 109, 112, 115, 118, 121
FokI	GGATG	3 cut(s)	111, 152, 293
Fsp4HI	GCNGC	9 cut(s)	85, 88, 91, 94, 109, 112, 115, 118, 121
GlaI	GCGC	1 cut(s)	483
GluI	GCNGC	9 cut(s)	85, 88, 91, 94, 109, 112, 115, 118, 121
HaeII	RGCGCY	1 cut(s)	485
HaeIII	GGCC	6 cut(s)	32, 42, 84, 132, 362, 456
HapII	CCGG	1 cut(s)	358
HgaI	GACGC	2 cut(s)	245, 409
HhaI	GCGC	1 cut(s)	484
Hin1I	GRCGYC	2 cut(s)	237, 401
Hin1II	CATG	1 cut(s)	154
Hin6I	GCGC	1 cut(s)	482
HinP1I	GCGC	1 cut(s)	482
HinfI	GANTC	3 cut(s)	473, 550, 625
HpaII	CCGG	1 cut(s)	358
HphI	GGTGA	1 cut(s)	295
Hpy166II	GTNNAC	1 cut(s)	472
Hpy188I	TCNGA	6 cut(s)	7, 311, 490, 549, 566, 630
Hpy188III	TCNNGA	4 cut(s)	151, 352, 574, 655
Hpy8I	GTNNAC	1 cut(s)	472
HpyAV	CCTTC	2 cut(s)	472, 607
HpyCH4III	ACNGT	2 cut(s)	215, 391
HpyCH4IV	ACGT	2 cut(s)	228, 432
HpyCH4V	TGCA	3 cut(s)	117, 123, 669
HpyF10VI	GCNNNNNNNGC	9 cut(s)	84, 90, 93, 99, 108, 114, 117, 120, 129
HpyF3I	CTNAG	1 cut(s)	627
HpySE526I	ACGT	2 cut(s)	228, 432
Hsp92I	GRCGYC	2 cut(s)	237, 401
Hsp92II	CATG	1 cut(s)	154
HspAI	GCGC	1 cut(s)	482
Ksp22I	TGATCA	1 cut(s)	570
KspI	CCGCGG	1 cut(s)	88
Kzo9I	GATC	5 cut(s)	327, 343, 373, 525, 570
Lsp1109I	GCAGC	6 cut(s)	95, 98, 101, 105, 107, 129
LweI	GCATC	3 cut(s)	89, 132, 357
MaeII	ACGT	2 cut(s)	228, 432
MaeIII	GTNAC	2 cut(s)	229, 391
MalI	GATC	5 cut(s)	329, 345, 375, 527, 572
MboI	GATC	5 cut(s)	327, 343, 373, 525, 570
MboII	GAAGA	5 cut(s)	118, 337, 517, 536, 547
MluCI	AATT	1 cut(s)	637
MlyI	GAGTC	2 cut(s)	467, 619
MmeI	TCCRAC	1 cut(s)	288
MnlI	CCTC	9 cut(s)	55, 63, 113, 254, 287, 299, 305, 311, 364
MspA1I	CMGCKG	3 cut(s)	87, 96, 120
MspI	CCGG	1 cut(s)	358
MspR9I	CCNGG	4 cut(s)	81, 170, 358, 359
Mva1269I	GAATGC	1 cut(s)	608
MvaI	CCWGG	2 cut(s)	81, 170
MvnI	CGCG	1 cut(s)	87
MwoI	GCNNNNNNNGC	9 cut(s)	84, 90, 93, 99, 108, 114, 117, 120, 129
NciI	CCSGG	2 cut(s)	358, 359
NdeII	GATC	5 cut(s)	327, 343, 373, 525, 570
NlaIII	CATG	1 cut(s)	154
NlaIV	GGNNCC	3 cut(s)	31, 43, 618
NmeAIII	GCCGAG	1 cut(s)	84
NmuCI	GTSAC	1 cut(s)	229
PagI	TCATGA	1 cut(s)	150
PcsI	WCGNNNNNNNCGW	1 cut(s)	348
PctI	GAATGC	1 cut(s)	608
PfeI	GAWTC	1 cut(s)	550
PkrI	GCNGC	9 cut(s)	86, 89, 92, 95, 110, 113, 116, 119, 122
Ple19I	CGATCG	1 cut(s)	346
PleI	GAGTC	2 cut(s)	467, 619
PpsI	GAGTC	2 cut(s)	467, 619
Psp6I	CCWGG	2 cut(s)	79, 168
PspEI	GGTNACC	1 cut(s)	391
PspGI	CCWGG	2 cut(s)	79, 168
PspN4I	GGNNCC	3 cut(s)	31, 43, 618
PspPI	GGNCC	3 cut(s)	30, 41, 360
PsrI	GAACNNNNNNTAC	2 cut(s)	17, 49
PstI	CTGCAG	1 cut(s)	119
PvuI	CGATCG	1 cut(s)	346
PvuII	CAGCTG	1 cut(s)	120
RsaI	GTAC	2 cut(s)	494, 511
RsaNI	GTAC	2 cut(s)	493, 510
SacII	CCGCGG	1 cut(s)	88
SatI	GCNGC	9 cut(s)	85, 88, 91, 94, 109, 112, 115, 118, 121
Sau3AI	GATC	5 cut(s)	327, 343, 373, 525, 570
Sau96I	GGNCC	3 cut(s)	30, 41, 360
ScaI	AGTACT	1 cut(s)	511
SchI	GAGTC	2 cut(s)	467, 619
ScrFI	CCNGG	4 cut(s)	81, 170, 358, 359
SetI	ASST	9 cut(s)	122, 147, 231, 291, 303, 383, 398, 435, 618
SfaNI	GCATC	3 cut(s)	89, 132, 357
SfcI	CTRYAG	1 cut(s)	115
Sfr303I	CCGCGG	1 cut(s)	88
SgrBI	CCGCGG	1 cut(s)	88
SmaI	CCCGGG	1 cut(s)	359
Sse9I	AATT	1 cut(s)	637
SsiI	CCGC	7 cut(s)	76, 85, 87, 90, 96, 156, 205
StyD4I	CCNGG	4 cut(s)	79, 168, 356, 357
StyI	CCWWGG	2 cut(s)	412, 451
TaaI	ACNGT	2 cut(s)	215, 391
TaiI	ACGT	2 cut(s)	231, 435
TaqI	TCGA	3 cut(s)	351, 476, 528
TasI	AATT	1 cut(s)	637
TatI	WGTACW	2 cut(s)	492, 509
TauI	GCSGC	3 cut(s)	87, 90, 93
TfiI	GAWTC	1 cut(s)	550
TseFI	GTSAC	1 cut(s)	229
TseI	GCWGC	6 cut(s)	93, 108, 111, 114, 117, 120
Tsp45I	GTSAC	1 cut(s)	229
TspDTI	ATGAA	1 cut(s)	175
TspGWI	ACGGA	2 cut(s)	248, 648
TspMI	CCCGGG	1 cut(s)	357
XapI	RAATTY	1 cut(s)	637
XmaI	CCCGGG	1 cut(s)	357
XmiI	GTMKAC	1 cut(s)	471
ZrmI	AGTACT	1 cut(s)	511

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.