Rh3AG200100

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001177
Physical Location & Seq
Reverse (-)
19696116 .. 19712367
16252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG200100.1

Sequence Viewer

Length: 807 bp
ATGGCAGAGAAAGAAGAAGCCCACTTAAACGGGGCATACTACGGCCCCTCAGTCCCGGCCAGCAAATCCTACCACCGTCCCAGCCGCGGCAGTGGCCCCCTCGGCTGCTGCTGCAGCTGCATCTTCGGCCTCGTCTTCAAAATCATCTTCGCAGCAGTCGTCTTCATGGGACTAGCCTTCCTCGTCTTCTGGCTCATAGTCCGCCCCACTAAAGTCAAGTTCCACGTCACCGACGCCACGCTCACCCAATTCAACTTCTCCACCGATAGCAACCTACATTACAACCTAGCCCTCAACCTCACCATTCGAAACCCCAACAAGAAGATCGGTGTCTACTACGACCGCATTGAAGCCAGAGCTCTTTACGAGGGCCAGAGGTTTGGTACGGTCACCCTCACGCCATTTTACCAAGGCCACAAAACCACAAACGTTTTGAACCCGGTGTTCCAGGGACAGCAATTGATTGTTGGATCCAACTTGCTTGAGGAGTTTAATCAACAGAAAAGTGCAGGGGTTTACGAGATTGAGATGAAGCTTTATTTGAGGATTCGGTTCAAGTTGGGTAGAATCAAGACTGGAAAGTTCAAGCCTAGAATTGAATGCGACTTGAAGGTTCCTTTGAACCAGACTGGGAATTCATCCTTTGTCCAAACGGCCGAGCGTGATAAATCTCAACTCCCACAATTGGCATTTGCCCATAATAGACTTGCGAAAAGAACCATCACAGAAGTCAAGCAAATAATGACTCGACCTCGATCGATGGGGCGCCATGGAAGCTACGAAGAAACATCCCCGCATTCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

268

Amino Acids

30.37

Weight (kDa)

9.76

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 100 - 196 1.1e-10 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 765
AccI GTMKAC 1 cut(s) 333
AccII CGCG 1 cut(s) 87
AciI CCGC 5 cut(s) 85, 87, 202, 343, 794
AclI AACGTT 1 cut(s) 429
AclWI GGATC 2 cut(s) 465, 478
AcoI YGGCCR 2 cut(s) 57, 654
AcsI RAATTY 1 cut(s) 634
AcyI GRCGYC 2 cut(s) 234, 766
AfaI GTAC 1 cut(s) 385
AfiI CCNNNNNNNGG 2 cut(s) 86, 685
AgsI TTSAA 9 cut(s) 139, 253, 350, 436, 556, 586, 599, 610, 622
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 4 cut(s) 117, 359, 535, 777
AluI AGCT 4 cut(s) 117, 359, 535, 777
Alw21I GWGCWC 1 cut(s) 361
AlwI GGATC 2 cut(s) 465, 478
AoxI GGCC 7 cut(s) 43, 57, 94, 127, 370, 412, 654
ApeKI GCWGC 6 cut(s) 105, 108, 111, 114, 117, 152
ApoI RAATTY 1 cut(s) 634
AspLEI GCGC 1 cut(s) 768
AspS9I GGNCC 3 cut(s) 44, 95, 370
AsuC2I CCSGG 2 cut(s) 56, 440
AsuHPI GGTGA 4 cut(s) 220, 235, 292, 382
AsuII TTCGAA 1 cut(s) 307
BamHI GGATCC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 765
BanII GRGCYC 1 cut(s) 361
BbsI GAAGAC 3 cut(s) 127, 154, 178
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 6 cut(s) 92, 95, 98, 104, 126, 164
BccI CCATC 2 cut(s) 728, 754
BceAI ACGGC 2 cut(s) 58, 669
BciT130I CCWGG 1 cut(s) 449
BcnI CCSGG 2 cut(s) 56, 440
BfaI CTAG 3 cut(s) 173, 287, 591
BfmI CTRYAG 1 cut(s) 112
BfoI RGCGCY 1 cut(s) 769
BglI GCCNNNNNGGC 1 cut(s) 102
BisI GCNGC 8 cut(s) 85, 88, 106, 109, 112, 115, 118, 153
BlsI GCNGC 8 cut(s) 86, 89, 107, 110, 113, 116, 119, 154
Bme1390I CCNGG 3 cut(s) 56, 440, 449
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 3 cut(s) 44, 95, 370
BmiI GGNNCC 5 cut(s) 46, 97, 472, 615, 767
BmrFI CCNGG 3 cut(s) 56, 440, 449
BmrI ACTGGG 1 cut(s) 639
BmsI GCATC 1 cut(s) 129
BmuI ACTGGG 1 cut(s) 639
BpiI GAAGAC 3 cut(s) 127, 154, 178
Bpu14I TTCGAA 1 cut(s) 307
BpuEI CTTGAG 1 cut(s) 503
BpuMI CCSGG 2 cut(s) 56, 440
Bsa29I ATCGAT 1 cut(s) 758
BsaHI GRCGYC 2 cut(s) 234, 766
BsaJI CCNNGG 5 cut(s) 85, 100, 409, 448, 769
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 685
Bse1I ACTGG 2 cut(s) 580, 634
BseBI CCWGG 1 cut(s) 449
BseCI ATCGAT 1 cut(s) 758
BseDI CCNNGG 5 cut(s) 85, 100, 409, 448, 769
BseGI GGATG 2 cut(s) 638, 788
BseLI CCNNNNNNNGG 2 cut(s) 86, 685
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 2 cut(s) 580, 634
BseRI GAGGAG 1 cut(s) 500
BseX3I CGGCCG 1 cut(s) 654
BseXI GCAGC 6 cut(s) 92, 95, 98, 104, 126, 164
BseYI CCCAGC 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 528
Bsh1236I CGCG 1 cut(s) 87
Bsh1285I CGRYCG 3 cut(s) 343, 657, 758
BshFI GGCC 7 cut(s) 45, 59, 96, 129, 372, 414, 656
BshNI GGYRCC 1 cut(s) 765
BshVI ATCGAT 1 cut(s) 758
BsiEI CGRYCG 3 cut(s) 343, 657, 758
BsiHKAI GWGCWC 1 cut(s) 361
BsiSI CCGG 2 cut(s) 56, 440
BslFI GGGAC 4 cut(s) 38, 63, 183, 465
BslI CCNNNNNNNGG 2 cut(s) 86, 685
BsmFI GGGAC 4 cut(s) 38, 63, 183, 465
BsmI GAATGC 2 cut(s) 605, 796
BsnI GGCC 7 cut(s) 45, 59, 96, 129, 372, 414, 656
Bsp119I TTCGAA 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 3 cut(s) 324, 470, 755
Bsp19I CCATGG 1 cut(s) 769
BspACI CCGC 5 cut(s) 85, 87, 202, 343, 794
BspANI GGCC 7 cut(s) 45, 59, 96, 129, 372, 414, 656
BspCNI CTCAG 1 cut(s) 62
BspDI ATCGAT 1 cut(s) 758
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 5 cut(s) 46, 97, 472, 615, 767
BspMAI CTGCAG 1 cut(s) 116
BspPI GGATC 2 cut(s) 465, 478
BspT104I TTCGAA 1 cut(s) 307
BspT107I GGYRCC 1 cut(s) 765
BsrI ACTGG 2 cut(s) 580, 634
BssECI CCNNGG 5 cut(s) 85, 100, 409, 448, 769
BssMI GATC 3 cut(s) 324, 470, 755
BssNI GRCGYC 2 cut(s) 234, 766
BssT1I CCWWGG 2 cut(s) 409, 769
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 2 cut(s) 77, 388
BstACI GRCGYC 2 cut(s) 234, 766
BstBI TTCGAA 1 cut(s) 307
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 1 cut(s) 49
BstDSI CCRYGG 2 cut(s) 85, 769
BstEII GGTNACC 1 cut(s) 388
BstF5I GGATG 2 cut(s) 638, 788
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 1 cut(s) 769
BstHHI GCGC 1 cut(s) 768
BstKTI GATC 3 cut(s) 327, 473, 758
BstMBI GATC 3 cut(s) 324, 470, 755
BstMCI CGRYCG 3 cut(s) 343, 657, 758
BstMWI GCNNNNNNNGC 7 cut(s) 93, 102, 111, 114, 117, 126, 774
BstNI CCWGG 1 cut(s) 449
BstPI GGTNACC 1 cut(s) 388
BstSCI CCNGG 3 cut(s) 54, 438, 447
BstSFI CTRYAG 1 cut(s) 112
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 6 cut(s) 92, 95, 98, 104, 126, 164
BstV2I GAAGAC 3 cut(s) 127, 154, 178
BstX2I RGATCY 1 cut(s) 470
BstXI CCANNNNNNTGG 1 cut(s) 380
BstYI RGATCY 1 cut(s) 470
BstZI CGGCCG 1 cut(s) 654
Bsu15I ATCGAT 1 cut(s) 758
BsuRI GGCC 7 cut(s) 45, 59, 96, 129, 372, 414, 656
BsuTUI ATCGAT 1 cut(s) 758
BtgI CCRYGG 2 cut(s) 85, 769
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 2 cut(s) 638, 788
BtsI GCAGTG 1 cut(s) 97
BtsIMutI CAGTG 1 cut(s) 97
Cac8I GCNNGC 1 cut(s) 61
CfoI GCGC 1 cut(s) 768
Cfr13I GGNCC 3 cut(s) 44, 95, 370
Cfr42I CCGCGG 1 cut(s) 88
ClaI ATCGAT 1 cut(s) 758
CseI GACGC 1 cut(s) 242
Csp6I GTAC 1 cut(s) 384
CviAII CATG 2 cut(s) 166, 770
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 1 cut(s) 49
DinI GGCGCC 1 cut(s) 767
DpnI GATC 3 cut(s) 326, 472, 757
DpnII GATC 3 cut(s) 324, 470, 755
EaeI YGGCCR 2 cut(s) 57, 654
EagI CGGCCG 1 cut(s) 654
EciI GGCGGA 1 cut(s) 191
Ecl136II GAGCTC 1 cut(s) 359
EclXI CGGCCG 1 cut(s) 654
Eco130I CCWWGG 2 cut(s) 409, 769
Eco24I GRGCYC 1 cut(s) 361
Eco52I CGGCCG 1 cut(s) 654
Eco53kI GAGCTC 1 cut(s) 359
Eco91I GGTNACC 1 cut(s) 388
EcoICRI GAGCTC 1 cut(s) 359
EcoO65I GGTNACC 1 cut(s) 388
EcoRI GAATTC 1 cut(s) 634
EcoRII CCWGG 1 cut(s) 447
EcoT14I CCWWGG 2 cut(s) 409, 769
EcoT38I GRGCYC 1 cut(s) 361
EgeI GGCGCC 1 cut(s) 767
EheI GGCGCC 1 cut(s) 767
ErhI CCWWGG 2 cut(s) 409, 769
FaeI CATG 2 cut(s) 169, 773
FaiI YATR 5 cut(s) 37, 167, 197, 699, 771
FaqI GGGAC 4 cut(s) 38, 63, 183, 465
FatI CATG 2 cut(s) 165, 769
FauI CCCGC 1 cut(s) 801
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 8 cut(s) 85, 88, 106, 109, 112, 115, 118, 153
FokI GGATG 2 cut(s) 625, 775
FriOI GRGCYC 1 cut(s) 361
Fsp4HI GCNGC 8 cut(s) 85, 88, 106, 109, 112, 115, 118, 153
FspBI CTAG 3 cut(s) 173, 287, 591
GlaI GCGC 1 cut(s) 767
GluI GCNGC 8 cut(s) 85, 88, 106, 109, 112, 115, 118, 153
GsaI CCCAGC 1 cut(s) 84
HaeII RGCGCY 1 cut(s) 769
HaeIII GGCC 7 cut(s) 45, 59, 96, 129, 372, 414, 656
HapII CCGG 2 cut(s) 56, 440
HgaI GACGC 1 cut(s) 242
HhaI GCGC 1 cut(s) 768
Hin1I GRCGYC 2 cut(s) 234, 766
Hin1II CATG 2 cut(s) 169, 773
Hin6I GCGC 1 cut(s) 766
HinP1I GCGC 1 cut(s) 766
HindIII AAGCTT 1 cut(s) 533
HinfI GANTC 3 cut(s) 547, 567, 745
HpaII CCGG 2 cut(s) 56, 440
HphI GGTGA 4 cut(s) 220, 235, 292, 382
Hpy166II GTNNAC 2 cut(s) 334, 517
Hpy188III TCNNGA 1 cut(s) 571
Hpy8I GTNNAC 2 cut(s) 334, 517
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 2 cut(s) 187, 604
HpyCH4III ACNGT 2 cut(s) 77, 388
HpyCH4IV ACGT 2 cut(s) 225, 429
HpyCH4V TGCA 3 cut(s) 114, 120, 509
HpyF10VI GCNNNNNNNGC 7 cut(s) 93, 102, 111, 114, 117, 126, 774
HpyF3I CTNAG 1 cut(s) 49
HpySE526I ACGT 2 cut(s) 225, 429
Hsp92I GRCGYC 2 cut(s) 234, 766
Hsp92II CATG 2 cut(s) 169, 773
HspAI GCGC 1 cut(s) 766
KasI GGCGCC 1 cut(s) 765
KspI CCGCGG 1 cut(s) 88
Kzo9I GATC 3 cut(s) 324, 470, 755
Lsp1109I GCAGC 6 cut(s) 92, 95, 98, 104, 126, 164
LweI GCATC 1 cut(s) 129
MaeI CTAG 3 cut(s) 173, 287, 591
MaeII ACGT 2 cut(s) 225, 429
MaeIII GTNAC 2 cut(s) 226, 388
MalI GATC 3 cut(s) 326, 472, 757
MboI GATC 3 cut(s) 324, 470, 755
MboII GAAGA 8 cut(s) 26, 115, 127, 139, 154, 178, 334, 794
MfeI CAATTG 2 cut(s) 458, 683
MflI RGATCY 1 cut(s) 470
MhlI GDGCHC 1 cut(s) 361
MluCI AATT 6 cut(s) 248, 458, 594, 634, 683, 802
Mly113I GGCGCC 1 cut(s) 766
MlyI GAGTC 1 cut(s) 739
MmeI TCCRAC 2 cut(s) 448, 498
MseI TTAA 2 cut(s) 26, 492
MspA1I CMGCKG 2 cut(s) 87, 117
MspI CCGG 2 cut(s) 56, 440
MspR9I CCNGG 3 cut(s) 56, 440, 449
MunI CAATTG 2 cut(s) 458, 683
Mva1269I GAATGC 2 cut(s) 605, 796
MvaI CCWGG 1 cut(s) 449
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 7 cut(s) 93, 102, 111, 114, 117, 126, 774
NarI GGCGCC 1 cut(s) 766
NciI CCSGG 2 cut(s) 56, 440
NcoI CCATGG 1 cut(s) 769
NdeII GATC 3 cut(s) 324, 470, 755
NlaIII CATG 2 cut(s) 169, 773
NlaIV GGNNCC 5 cut(s) 46, 97, 472, 615, 767
NmeAIII GCCGAG 2 cut(s) 81, 682
NmuCI GTSAC 2 cut(s) 226, 388
NspV TTCGAA 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 2 cut(s) 605, 796
PfeI GAWTC 2 cut(s) 547, 567
PkrI GCNGC 8 cut(s) 86, 89, 107, 110, 113, 116, 119, 154
Ple19I CGATCG 1 cut(s) 758
PleI GAGTC 1 cut(s) 739
PluTI GGCGCC 1 cut(s) 769
PpsI GAGTC 1 cut(s) 739
Psp124BI GAGCTC 1 cut(s) 361
Psp1406I AACGTT 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 447
PspEI GGTNACC 1 cut(s) 388
PspFI CCCAGC 1 cut(s) 80
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 5 cut(s) 46, 97, 472, 615, 767
PspPI GGNCC 3 cut(s) 44, 95, 370
PstI CTGCAG 1 cut(s) 116
PsuI RGATCY 1 cut(s) 470
PvuI CGATCG 1 cut(s) 758
PvuII CAGCTG 1 cut(s) 117
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
SacI GAGCTC 1 cut(s) 361
SacII CCGCGG 1 cut(s) 88
SaqAI TTAA 2 cut(s) 26, 492
SatI GCNGC 8 cut(s) 85, 88, 106, 109, 112, 115, 118, 153
Sau3AI GATC 3 cut(s) 324, 470, 755
Sau96I GGNCC 3 cut(s) 44, 95, 370
SchI GAGTC 1 cut(s) 739
ScrFI CCNGG 3 cut(s) 56, 440, 449
SduI GDGCHC 1 cut(s) 361
SfaNI GCATC 1 cut(s) 129
SfcI CTRYAG 1 cut(s) 112
SfoI GGCGCC 1 cut(s) 767
Sfr303I CCGCGG 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 307
SgrBI CCGCGG 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 6 cut(s) 248, 458, 594, 634, 683, 802
SsiI CCGC 5 cut(s) 85, 87, 202, 343, 794
SspDI GGCGCC 1 cut(s) 765
SspMI CTAG 3 cut(s) 173, 287, 591
SstI GAGCTC 1 cut(s) 361
StyD4I CCNGG 3 cut(s) 54, 438, 447
StyI CCWWGG 2 cut(s) 409, 769
TaaI ACNGT 2 cut(s) 77, 388
TaiI ACGT 2 cut(s) 228, 432
TaqI TCGA 4 cut(s) 307, 748, 754, 758
TasI AATT 6 cut(s) 248, 458, 594, 634, 683, 802
TauI GCSGC 2 cut(s) 87, 90
TfiI GAWTC 2 cut(s) 547, 567
Tru1I TTAA 2 cut(s) 26, 492
Tru9I TTAA 2 cut(s) 26, 492
TscAI CASTG 1 cut(s) 97
TseFI GTSAC 2 cut(s) 226, 388
TseI GCWGC 6 cut(s) 105, 108, 111, 114, 117, 152
Tsp45I GTSAC 2 cut(s) 226, 388
TspDTI ATGAA 3 cut(s) 154, 545, 627
TspRI CASTG 1 cut(s) 97
XapI RAATTY 1 cut(s) 634
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 3 cut(s) 173, 287, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.