Rorug03G0150500

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001177
Physical Location & Seq
Forward (+)
12415066 .. 12416668
1603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0150500.1

Sequence Viewer

Length: 495 bp
ATGTCTAACGCAGTGACTCTGCCGTGTCTCGGCATCGTCAGCATGGCGCGCCGCCCAAAACGGACGGCAAAGACTAAGCAAATCCCACCGCCGACTAGCGTCGGCTTTGGAATGAGCAAGAGGAAGGAGCCGCTCTGGCAATGCGTGGAGGGTTGCGGAGCTTGCTGCAAACTCGACAAGGGTCCCTCTTTCGCTACCCCTGAAGAAATCTTCACCGACCCTGCCGACGTTGAGCTTTACAGAAGTATGGTGGGTTCGGATGGGTGGTGCGTGCACTTTGAGAAAAGCACACGCAAGTGCTCCATTTACAATGAGCGTCCATATTTTTGTTGCGTGGAGGCAGACGTGTTTGACAAACTGTATGGGATTAGCAAGAAGAAATTCAATGTGGAAGCTTGCAGGAGTTGCACGGACACCATCAAGTCTGTTTATGGTCCCTATTCCAAGGAACTGGGTCACTACAATAGCACAGTAATGAGCTCCAGTTCTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

164

Amino Acids

18.2

Weight (kDa)

8.74

Isoelectric Point (pI)

53.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CxxCxxCC PF03692 47 - 134 2.4e-09 Putative zinc- or iron-chelating domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 133
AccII CGCG 1 cut(s) 49
AciI CCGC 4 cut(s) 52, 89, 131, 156
AcsI RAATTY 1 cut(s) 380
AcuI CTGAAG 1 cut(s) 222
AfiI CCNNNNNNNGG 1 cut(s) 29
AflIII ACRYGT 1 cut(s) 345
AgsI TTSAA 1 cut(s) 385
AjiI CACGTC 1 cut(s) 346
AleI CACNNNNGTG 1 cut(s) 295
AluBI AGCT 4 cut(s) 161, 235, 395, 480
AluI AGCT 4 cut(s) 161, 235, 395, 480
Alw21I GWGCWC 3 cut(s) 276, 302, 482
Alw26I GTCTC 1 cut(s) 32
Alw44I GTGCAC 1 cut(s) 272
ApaLI GTGCAC 1 cut(s) 272
ApeKI GCWGC 1 cut(s) 165
ApoI RAATTY 1 cut(s) 380
AscI GGCGCGCC 1 cut(s) 47
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 2 cut(s) 49, 51
AspS9I GGNCC 2 cut(s) 182, 434
AsuHPI GGTGA 1 cut(s) 205
AvaII GGWCC 2 cut(s) 182, 434
BaeGI GKGCMC 1 cut(s) 276
BanII GRGCYC 1 cut(s) 482
Bbv12I GWGCWC 3 cut(s) 276, 302, 482
BbvI GCAGC 1 cut(s) 152
BccI CCATC 2 cut(s) 254, 425
BceAI ACGGC 2 cut(s) 7, 81
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 2 cut(s) 96, 489
BglI GCCNNNNNGGC 1 cut(s) 136
BisI GCNGC 3 cut(s) 52, 131, 166
BlsI GCNGC 3 cut(s) 53, 132, 167
Bme18I GGWCC 2 cut(s) 182, 434
BmgBI CACGTC 1 cut(s) 346
BmgT120I GGNCC 2 cut(s) 182, 434
BmiI GGNNCC 4 cut(s) 129, 183, 184, 436
BmrI ACTGGG 1 cut(s) 461
BmsI GCATC 1 cut(s) 42
BmuI ACTGGG 1 cut(s) 461
BoxI GACNNNNGTC 2 cut(s) 98, 180
BpmI CTGGAG 1 cut(s) 466
BsaJI CCNNGG 1 cut(s) 444
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse1I ACTGG 2 cut(s) 456, 483
Bse3DI GCAATG 1 cut(s) 146
BseDI CCNNGG 1 cut(s) 444
BseGI GGATG 1 cut(s) 265
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMI GCAATG 1 cut(s) 146
BseNI ACTGG 2 cut(s) 456, 483
BsePI GCGCGC 1 cut(s) 47
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 1 cut(s) 152
Bsh1236I CGCG 1 cut(s) 49
BsiHKAI GWGCWC 3 cut(s) 276, 302, 482
BslFI GGGAC 2 cut(s) 168, 420
BslI CCNNNNNNNGG 1 cut(s) 29
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 168, 420
Bsp1286I GDGCHC 3 cut(s) 276, 302, 482
BspACI CCGC 4 cut(s) 52, 89, 131, 156
BspFNI CGCG 1 cut(s) 49
BspLI GGNNCC 4 cut(s) 129, 183, 184, 436
BsrBI CCGCTC 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 146
BsrI ACTGG 2 cut(s) 456, 483
BssECI CCNNGG 1 cut(s) 444
BssHII GCGCGC 1 cut(s) 47
BssT1I CCWWGG 1 cut(s) 444
Bst4CI ACNGT 2 cut(s) 360, 472
BstAPI GCANNNNNTGC 1 cut(s) 405
BstC8I GCNNGC 4 cut(s) 49, 163, 272, 397
BstDEI CTNAG 1 cut(s) 75
BstF5I GGATG 1 cut(s) 265
BstFNI CGCG 1 cut(s) 49
BstHHI GCGC 2 cut(s) 49, 51
BstMAI GTCTC 1 cut(s) 32
BstMWI GCNNNNNNNGC 5 cut(s) 39, 48, 136, 162, 405
BstPAI GACNNNNGTC 2 cut(s) 98, 180
BstSLI GKGCMC 1 cut(s) 276
BstUI CGCG 1 cut(s) 49
BstV1I GCAGC 1 cut(s) 152
BstXI CCANNNNNNTGG 1 cut(s) 451
BtrI CACGTC 1 cut(s) 346
BtsCI GGATG 1 cut(s) 265
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 4 cut(s) 49, 163, 272, 397
CfoI GCGC 2 cut(s) 49, 51
Cfr13I GGNCC 2 cut(s) 182, 434
CseI GACGC 2 cut(s) 88, 305
CviAII CATG 1 cut(s) 43
CviJI RGCY 6 cut(s) 105, 130, 161, 235, 395, 480
CviKI_1 RGCY 6 cut(s) 105, 130, 161, 235, 395, 480
DdeI CTNAG 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 480
Eco130I CCWWGG 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 482
Eco47I GGWCC 2 cut(s) 182, 434
Eco53kI GAGCTC 1 cut(s) 480
Eco57I CTGAAG 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 480
EcoO109I RGGNCCY 1 cut(s) 182
EcoT14I CCWWGG 1 cut(s) 444
EcoT38I GRGCYC 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 1 cut(s) 46
FaiI YATR 5 cut(s) 44, 248, 322, 363, 432
FaqI GGGAC 2 cut(s) 168, 420
FatI CATG 1 cut(s) 42
Fnu4HI GCNGC 3 cut(s) 52, 131, 166
FokI GGATG 1 cut(s) 272
FriOI GRGCYC 1 cut(s) 482
Fsp4HI GCNGC 3 cut(s) 52, 131, 166
FspBI CTAG 2 cut(s) 96, 489
GlaI GCGC 2 cut(s) 48, 50
GluI GCNGC 3 cut(s) 52, 131, 166
GsuI CTGGAG 1 cut(s) 466
HgaI GACGC 2 cut(s) 88, 305
HhaI GCGC 2 cut(s) 49, 51
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 2 cut(s) 47, 49
HinP1I GCGC 2 cut(s) 47, 49
HindIII AAGCTT 1 cut(s) 393
HinfI GANTC 1 cut(s) 16
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 1 cut(s) 259
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 2 cut(s) 104, 230
HpyAV CCTTC 1 cut(s) 118
HpyCH4III ACNGT 2 cut(s) 360, 472
HpyCH4IV ACGT 2 cut(s) 228, 345
HpyCH4V TGCA 4 cut(s) 168, 274, 399, 408
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 48, 136, 162, 405
HpyF3I CTNAG 1 cut(s) 75
HpySE526I ACGT 2 cut(s) 228, 345
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 2 cut(s) 47, 49
KflI GGGWCCC 1 cut(s) 182
LmnI GCTCC 4 cut(s) 127, 158, 305, 485
LpnPI CCDG 5 cut(s) 121, 213, 234, 385, 437
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 1 cut(s) 42
MaeI CTAG 2 cut(s) 96, 489
MaeII ACGT 2 cut(s) 228, 345
MaeIII GTNAC 2 cut(s) 13, 455
MbiI CCGCTC 1 cut(s) 133
MboII GAAGA 3 cut(s) 202, 215, 388
MhlI GDGCHC 3 cut(s) 276, 302, 482
MluCI AATT 1 cut(s) 380
MlyI GAGTC 1 cut(s) 10
MnlI CCTC 4 cut(s) 114, 142, 196, 331
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 1 cut(s) 493
MslI CAYNNNNRTG 2 cut(s) 295, 473
MvnI CGCG 1 cut(s) 49
MwoI GCNNNNNNNGC 5 cut(s) 39, 48, 136, 162, 405
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 4 cut(s) 129, 183, 184, 436
NmeAIII GCCGAG 1 cut(s) 9
NmuCI GTSAC 2 cut(s) 13, 455
OliI CACNNNNGTG 1 cut(s) 295
PalAI GGCGCGCC 1 cut(s) 47
PauI GCGCGC 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 380
PkrI GCNGC 3 cut(s) 53, 132, 167
PleI GAGTC 1 cut(s) 10
PpsI GAGTC 1 cut(s) 10
PpuMI RGGWCCY 1 cut(s) 182
PshAI GACNNNNGTC 2 cut(s) 98, 180
Psp124BI GAGCTC 1 cut(s) 482
Psp5II RGGWCCY 1 cut(s) 182
PspN4I GGNNCC 4 cut(s) 129, 183, 184, 436
PspPI GGNCC 2 cut(s) 182, 434
PspPPI RGGWCCY 1 cut(s) 182
PsrI GAACNNNNNNTAC 2 cut(s) 238, 270
PteI GCGCGC 1 cut(s) 47
RseI CAYNNNNRTG 2 cut(s) 295, 473
SacI GAGCTC 1 cut(s) 482
SaqAI TTAA 1 cut(s) 493
SatI GCNGC 3 cut(s) 52, 131, 166
Sau96I GGNCC 2 cut(s) 182, 434
SchI GAGTC 1 cut(s) 10
SduI GDGCHC 3 cut(s) 276, 302, 482
SetI ASST 6 cut(s) 163, 231, 237, 348, 397, 482
SfaNI GCATC 1 cut(s) 42
SgsI GGCGCGCC 1 cut(s) 47
SinI GGWCC 2 cut(s) 182, 434
SmiMI CAYNNNNRTG 2 cut(s) 295, 473
Sse9I AATT 1 cut(s) 380
SsiI CCGC 4 cut(s) 52, 89, 131, 156
SspMI CTAG 2 cut(s) 96, 489
SstI GAGCTC 1 cut(s) 482
StyI CCWWGG 1 cut(s) 444
TaaI ACNGT 2 cut(s) 360, 472
TaiI ACGT 2 cut(s) 231, 348
TaqI TCGA 1 cut(s) 174
TasI AATT 1 cut(s) 380
TauI GCSGC 2 cut(s) 54, 133
Tru1I TTAA 1 cut(s) 493
Tru9I TTAA 1 cut(s) 493
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 2 cut(s) 13, 455
TseI GCWGC 1 cut(s) 165
Tsp45I GTSAC 2 cut(s) 13, 455
TspGWI ACGGA 2 cut(s) 76, 425
TspRI CASTG 1 cut(s) 18
VneI GTGCAC 1 cut(s) 272
VpaK11BI GGWCC 2 cut(s) 182, 434
XapI RAATTY 1 cut(s) 380
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 2 cut(s) 96, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.